MPM / Oliver Tambo Institute of Governance and Policy Studies

MSc (Microbiology) / Department of Microbiology / BACKGROUND: Antibiotic resistance amongst microbial pathogens has become a challenge over past decades, bringing about genuine and frequently deadly contaminations that can't be dealt with by ordinary means. This has led to a search on developing solutions to this problem by searching for new source of antimicrobial agents or chemically altering the existing ones. Traditional medicinal plants and nanoparticles are highly targeted as promising agents to address the challenge. Pyrenacantha grandiflora Baill from Icacenaceae family possess pharmaceutical activities and is used by Vhavenda people to cure gastrointestinal related infections, diarrhea and tooth pain. OBJECTIVES: The present study aimed to synthesize, characterize and evaluate the efficacy of Pyranacantha grandiflora extracts alone and when conjugated with selected nanoparticles against pathogenic microorganisms. Furthermore, this study investigated the efficacy of selected antibiotics when conjugated with nanoparticles against selected pathogenic microbes. METHODS: Pyrenacantha grandiflora Baill (tubers) were collected from Masisi area. Bioactive compounds were extracted using different solvents such as methanol, acetone, hot water, dichloromethane and chloroform. Preliminary phytochemical screening was done to identify different phytochemicals in the extracts and their functional groups were identified by Fourier Transform Infrared spectroscopy (FTIR). Extracts were further assessed for their total phenolic and flavonoids content. Thin layer chromatography was used to separate the compounds from the plant extracts and active compounds/group of compounds were identified by bioautography. The antioxidant ability of the extracts to scavenge free radical DPPH was also determined. Silver and gold nanoparticles were synthesized using chemical and biological methods, characterized by Ultraviolet-Visible Spectrophotometry (UV-VIS), Transmission Electron Microscopy (TEM) and Energy dispersive X-ray analysis (EDX). Plant extracts, nanoparticles and antibiotics were xix conjugated differently, and conjugates were analyzed by FTIR and their antimicrobial activities were evaluated against different bacteria and fungi. The conjugates were tested for antimicrobial activity against extended beta-lactamase producing Escherichia coli (ATCC 35218), Escherichia coli (ATCC 25922), methicillin-resistant Staphylococcus aureus (ATCC 25923), methicillin-susceptible Staphylococcus aureus (ATCC 33591) and beta-lactamase producing Klebsiella pneumonia (ATCC 700603) using agar diffusion assay and the minimum inhibitory concentrations (MIC) were determined using the microdilution method. The minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined by sub-culturing from the MIC plates on Mueller-Hinton Agar. RESULTS: Pyrenacantha grandiflora was found to contain phenolics, saponins, alkaloids, tannin, steroids, terpenoids and flavonoids. FTIR spectroscopic studies revealed different characteristic peak values with various functional compounds similar in most extracts but differed with transmittance values. The total phenolic contents in the examined extracts ranged from 14.167 to 19.02 mg GA/g. The total flavonoid content in the examined extracts ranged from 26.603 to 34.621 mg QE/g. Thin-layer chromatography revealed various Rf values and when analyzed with bioautography, well-defined inhibition zones within the Rf value of 0.236 was identified against E. coli and K. pneumonia. The MICs of the extracts were determined, and all the extracts showed some antimicrobial activity against all tested strains ranging from 0.06-7.5 mg ml/g. Some extracts appeared to be fungicidal and hot water extracts were more active against Cryptococcus neoformans with the MFC value of 0.06 mg/ml. The methanol extract was also active against most tested strains including Candida tropicalis with the minimum fungicidal concentration value of 3.75 mg/ml. Pyrenacantha grandiflora tuber extracts conjugated with silver or gold nanoparticles exhibited a good antibacterial activity against all bacterial strain used and very few were able to exhibit bactericidal activity. Penicillin showed improvement of antibacterial activity xx when conjugated with compounds from the acetone extracts and vancomycin was found to be more effective when conjugated with silver nanoparticles and water extracts. CONCLUSION: The present study validated the efficacy of conjugated P. grandiflora tuber extracts which is used in traditional medicine. The results revealed that water extracts which are generally used by the traditional healers are active against most microorganisms tested as well as methanol and acetone extracts and the synergistic effect was observed when they were conjugated to gold and silver nanoparticles. The results of the present investigation clearly indicate that antimicrobial activity of Pyrenacantha grandiflora Baill tuber when conjugated with selected nanoparticles and antibiotics vary with test strain and the type of solvent used during extraction, thus giving hope for future development of drug leads. / NRF

No description available.

A study of the relevant theoretical concepts and empirical research was undertaken to explore the role of the social worker pertaining to the problem of epilepsy in the black community of Mamelodi. The sample comprised 40 families in which there was a person with epilepsy. Data were obtained through an interview schedule administered by the researcher. The literature study highlighted the phenomenon epilepsy, causes of epilepsy/ psychosocial effects of epilepsy on the epileptic and his family and the treatment of the epileptic. However/ it became quite clear that a multi-disciplinary team approach is vital importance in the treatment of an epileptic Furthermore/ significant others like traditional healers, faith healers, priests, relatives and friends were identified as some of the important informal support systems. Although the findings of study cannot be generalised to the entire population they can be of great significance for further research. / 'n Studie van die relevante konsepte asook empiriese navorsing is gedoen om die rol van die maatskaplike werker met betrekking tot die probleem van epilepsie in die swart gemeenskap van Mamelodi te eksploreer. Die steekproef is saamgestel uit 'n groep van 40 gesinne met 'n epileptikus in hul midde. Inlighting is verkry deur groep van onderhoudskedule. Die literatuurstudie beklemtoon die volgende: die verskynsel van epilepsie, oorsake van epilepsie, psigososiale gevolge van epilepsie op die per soon met epilepsie en sy gesin en die behandeling van die epileptikus. Dit het egter duidelik na vore gekom dat 'n multi-dissiplinere spanbenadering van die uiterste belang is in die behandeling van die epilepsielyer. Dit het ook duidelik geword dat betekenisvolle ander persone soos tradisionele genesers, geloofsgenesers priesters, familie en vriende van die mees belangrike steunstelsels is. Alhoewel bevindinge van die studie nie veralgemeen kan word en van toepassing gemaak kan word op die bevolking as geheel nie, kan dit egter van groot belang vir verdere navorsing wees. / Social Work / M.A. (Social Science)

The purpose of the study was to explore and describe the existing relationship between traditional healers and nurse practitioners in Maseru Health Service Area in Lesotho and also to determine why people consult traditional healers. Qualitative and quantitative methods were used to investigate the relationship between traditional healers and nurse practitioners and also to determine why people utilize the services of traditional healers. The study was limited to Maseru Health Service Area in Lesotho. Data was collected from twenty-seven (27) nurses from nineteen (19) clinics and from thirty (30) traditional healers from the same health service area. Data from traditional healers was collected using semi-structured interviews while nurse practitioners were given a questionnaire to complete. The study revealed that there was no formal relationship between traditional healers and nurse practitioners. Support for traditional healers was revealed to be limited, for example, only four (14.8%) nurses had a programme for traditional healers while twenty ( 66.7%) traditional healers did not have any contact with nurse practitioners. Involvement of traditional healers in primary health care at grassroots level has therefore been very minimal. The reasons why people utilize traditional healers were found to be as follows: • When people think they have been bewitched. • Traditional healers can tell the actual cause of disease • Failure of modern practice • For social problems According to the study, both traditional healers and nurse practitioners felt that collaboration between traditional healers and nurse practitioners was essential because it would enable planned referral of patients from one group to another where necessary; it would also facilitate exchange of ideas and knowledge for the benefit of the people served. Recommendations on collaboration and on support systems are given in Chapter Six. / Health Studies / D. Litt. et Phil. (Nursing Science)

This study focuses on the use of the Athrixia phylicoides plant in Tshwane, Gauteng, South Africa. The plant is used within the domestic domain as a beverage, for medicinal purposes, as well as for the manufacturing of domestic brooms. The aim of the study was to investigate the use and market of Athrixia phylicoides in two of the Tshwane markets, namely; Denneboom and Marabastad. Case study design, participant observation and interviews were employed to collect data. A literature review was conducted to construct a theoretical framework. The study reveals that traditional healers, broom makers and customers have a remarkable knowledge about Athrixia phylicoides, which has been untapped thus far. In addition, the study indicates that possible extinction of the Athrixia phylicoides plant, due to over-harvesting and lack of conservation, will pose a challenge to the market. / Anthropology / M.A. (Anthropology)

A study of the relevant theoretical concepts and empirical research was undertaken to explore the role of the social worker pertaining to the problem of epilepsy in the black community of Mamelodi. The sample comprised 40 families in which there was a person with epilepsy. Data were obtained through an interview schedule administered by the researcher. The literature study highlighted the phenomenon epilepsy, causes of epilepsy/ psychosocial effects of epilepsy on the epileptic and his family and the treatment of the epileptic. However/ it became quite clear that a multi-disciplinary team approach is vital importance in the treatment of an epileptic Furthermore/ significant others like traditional healers, faith healers, priests, relatives and friends were identified as some of the important informal support systems. Although the findings of study cannot be generalised to the entire population they can be of great significance for further research. / 'n Studie van die relevante konsepte asook empiriese navorsing is gedoen om die rol van die maatskaplike werker met betrekking tot die probleem van epilepsie in die swart gemeenskap van Mamelodi te eksploreer. Die steekproef is saamgestel uit 'n groep van 40 gesinne met 'n epileptikus in hul midde. Inlighting is verkry deur groep van onderhoudskedule. Die literatuurstudie beklemtoon die volgende: die verskynsel van epilepsie, oorsake van epilepsie, psigososiale gevolge van epilepsie op die per soon met epilepsie en sy gesin en die behandeling van die epileptikus. Dit het egter duidelik na vore gekom dat 'n multi-dissiplinere spanbenadering van die uiterste belang is in die behandeling van die epilepsielyer. Dit het ook duidelik geword dat betekenisvolle ander persone soos tradisionele genesers, geloofsgenesers priesters, familie en vriende van die mees belangrike steunstelsels is. Alhoewel bevindinge van die studie nie veralgemeen kan word en van toepassing gemaak kan word op die bevolking as geheel nie, kan dit egter van groot belang vir verdere navorsing wees. / Social Work / M.A. (Social Science)

The purpose of the study was to explore and describe the existing relationship between traditional healers and nurse practitioners in Maseru Health Service Area in Lesotho and also to determine why people consult traditional healers. Qualitative and quantitative methods were used to investigate the relationship between traditional healers and nurse practitioners and also to determine why people utilize the services of traditional healers. The study was limited to Maseru Health Service Area in Lesotho. Data was collected from twenty-seven (27) nurses from nineteen (19) clinics and from thirty (30) traditional healers from the same health service area. Data from traditional healers was collected using semi-structured interviews while nurse practitioners were given a questionnaire to complete. The study revealed that there was no formal relationship between traditional healers and nurse practitioners. Support for traditional healers was revealed to be limited, for example, only four (14.8%) nurses had a programme for traditional healers while twenty ( 66.7%) traditional healers did not have any contact with nurse practitioners. Involvement of traditional healers in primary health care at grassroots level has therefore been very minimal. The reasons why people utilize traditional healers were found to be as follows: • When people think they have been bewitched. • Traditional healers can tell the actual cause of disease • Failure of modern practice • For social problems According to the study, both traditional healers and nurse practitioners felt that collaboration between traditional healers and nurse practitioners was essential because it would enable planned referral of patients from one group to another where necessary; it would also facilitate exchange of ideas and knowledge for the benefit of the people served. Recommendations on collaboration and on support systems are given in Chapter Six. / Health Studies / D. Litt. et Phil. (Nursing Science)

Sickness is a problem that has not escaped any society and thus is on the agenda of every culture. Since time immemorial cultures have searched for answers to the questions raised by the phenomenon of sickness but none have provided solutions, as it has become clear that sickness is part of our human existence. Many people have resorted to religion in search consolation in times of affliction and the Roman Catholic Church is not immune to this expectation, as we see many leaving the church in search of healing in the African Traditional Religions and other Christian churches because they feel that the church is inadequately dealing with the problem. In this study the author undertakes a research journey within the Diocese of Witbank of the RCC to investigate as to why the church’s healing ministry is not effective. Through engagement with participants in the research field and relevant literature the author discovered that the RCC is seen to be suspicious of the African worldview and consequently does not take its members’ fears and frustrations around the phenomenon of sickness serious as it judges them to be superstitious. This suggests that there is nothing that Western Christianity can learn from African cultures maintaining its superior attitude and further alienating indigenous communities. The author suggests that in order for the RCC to responds with relevance to this problem it needs to reconcile the Christian worldview, which is western, with the African worldview. He puts high on the agenda of Christian theology the urgent call to African theologians to develop an African theology that will give birth to a genuine African Christianity. In conclusion as a solution the author proposes an integrated and intercultural healing ministry for the Diocese of Witbank. This model is aimed at appropriating African values, idioms and language in the RCC to create an atmosphere where the church is seen as a welcome guest who comes bearing gifts but at the same time expects to be taken care of by its host. / Philosophy, Practical and Systematic Theology / M. Th. (Pastoral Therapy)

This thesis focuses on the significance of blood and the perception of the body in both learned and popular culture in order to investigate problems of identity and social exclusion in early modern Europe. Starting from the view of blood as a liminal matter, manifesting fertile, positive aspects in conjunction with dangerous, negative ones, I show how it was believed to attract supernatural forces within the natural world. It could empower or pollute, restore health or waste corporeal and spiritual existence. While this theme has been studied in a medieval religious context and by anthropologists, its relevance during the early modern period has not been explored. I argue that, considering the impact of the Reformation on people’s mentalities, studying the way in which ideas regarding blood and the body changed from late medieval times to the eighteenth century can provide new insights about patterns of social and religious tensions, such as the witch-trials and persecutions. In this regard the thesis engages with anthropological theories, comparing the dialectic between blood and body with that between identity and society, demonstrating that they both spread from the conflict of life with death, leading to the social embodiment or to the rejection of an individual. A comparative approach is also employed to analyze blood symbolism in Protestant and Catholic countries, and to discuss how beliefs were influenced by both cultural similarities and religious differences. Combining historical sources, such as witches’ confessions, with appropriate examples from anthropology I also examine a corpus of popular ideas, which resisted to theological and learned notions or slowly merged with them. Blood had different meanings for different sections of society, embodying both the physical struggle for life and the spiritual value of the Christian soul. Chapters 2, 3 and 4 develop the dualism of the fluid in late medieval and early modern ritual murder accusations against Jews, European witchcraft and supernatural beliefs and in the medical and philosophical knowledge, while chapters 5 and 6 focus on blood themes in Protestant England and in Counter-Reformation Italy. Through the examination of blood in these contexts I hope to demonstrate that contrasting feelings, fears and beliefs related to dangerous or extraordinary individuals, such as Jews, witches, and Catholic saints, but also superhuman beings such as fairies, vampires and werewolves, were rooted in the perception of the body as an unstable substance, that was at the base of ethnic, religious and gender stereotypes.