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Release of endomorphin (ENDO)-2 like substances from the dorsal horn of the isolated rat spinal cord was measured by the immobilized-antibody microprobe technique. Spinal cords were removed from anesthetized 4-6 week old rats and superfused with oxygenated Krebs solution at room temperature. Glass microprobes coated with ENDO-2 antibodies were inserted into the dorsal horn of the lumbar spinal cord 1.5 mm lateral to the midline to a depth 2.5 mm below the dorsal surface of the cord. Each probe remained in situ for 10 min periods before, during and after electrical stimulation applied to the dorsal root entry zone of the same spinal segment. There was no detectable basal release of immunoreactive endomorphin-2 like substance (irENDO) from the dorsal horns during the pre-stimulation, nor following the stimulation period. A significant release of irENDO was measured during the electrical stimulation. These results provide the first evidence of a irEndo release that is correlated spatially with the dorsal horn laminae I and II where ENDO-2-immunoreactive fibers are concentrated in the dorsal horn in response to electrical activation of primary afferent fibers.

Metastin, the product of metastasis suppressor gene KiSS-1, is proposed to be the natural ligand for the G-protein-coupled receptor GPR54, known also as AXOR12. This immunohistochemical study, using a rabbit polyclonal antiserum against the human metastin fragment (45-54)-NH2, showed that in rats metastin-like immunoreactivity (MTS-LI) was present in neurons of the nucleus of the solitary tract and caudoventrolateral reticular nucleus, and in cell processes of the spinal trigeminal tract and lateral reticular nucleus. MTS-LI was confined mainly to neurons and fibers at or caudal to the area postrema. In the spinal cord, MTS-LI cell processes formed a dense plexus in superficial layers I and II of the dorsal horn. The pattern of distribution of MTS-LI in the medulla and spinal cord suggests that this novel peptide may participate in autonomic and sensory neural signaling.

Metastin, the gene product of metastasis suppressor gene KiSS-1, is the endogenous ligand for the G-protein-coupled receptor GPR54 (or AXOR12, or OT7T175). The expression of KiSS-1 gene and peptide and the distribution of metastin were studied in the rat central nervous system by reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemical methods. KiSS-1 gene and peptide expression was higher in the hypothalamus than in the brainstem and spinal cord. In the brain, metastin-like immunoreactivity (irMT) was found mainly in three groups of cells: dorsomedial hypothalamic nucleus, nucleus of the solitary tract, and caudal ventrolateral medulla. Immunoreactive fibers of varying density were noted in bed nucleus of stria terminalis, septal nuclei, nucleus accumbens, caudate putamen, diagonal band, amygdala, hypothalamus, zona incerta, thalamus, periaqueductal gray, raphe nuclei, lateral parabrachial nucleus, locus coeruleus, spinal trigeminal tract, rostral ventrolateral medulla, and medullary reticular nucleus. Preabsorption of the antiserum with metastin peptide fragment (45-54)-NH2 (1 μg/ml) resulted in no staining in any of the sections. The biological activity of metastin was assessed by monitoring intracellular calcium [Ca2+]i in cultured hippocampal neurons, which are known to express GPR54. Metastin increased [Ca 2+]i in a population of cultured hippocampal neurons. The results show that metastin is biologically active in rat central neurons, and its anatomical distribution suggests a possible role in nociception and autonomic and neuroendocrine functions.

1. Although the ORL1 receptor is clearly located within the spinal cord, the functional signalling mechanism of the ORL1 receptor in the spinal cord has not been clearly documented. The present study was then to investigate the guanine nucleotide binding protein (G-protein) activation mediated through by the ORL1 receptor in the mouse spinal cord, measuring the modulation of guanosine-5'-o-(3-[35S]-thio) triphosphate ([35S]-GTPγS) binding by the putative endogenous ligand nociceptin, also referred as orphanin FQ. We also studied the anatomical distribution of nociceptin-like immunoreactivity and nociceptin-stimulated [35S]-GTPγS autoradiography in the spinal cord. 2. Immunohistochemical staining of mouse spinal cord sections revealed a dense plexus of nociceptin-like immunoreactive fibres in the superficial layers of the dorsal horn throughout the entire length of the spinal cord. In addition, networks of fibres were seen projecting from the lateral border of the dorsal horn to the lateral grey matter and around the central canal. 3. In vitro [35S]-GTPγS autoradiography showed high levels of nociceptin-stimulated [35S]-GTPγS binding in the superficial layers of the mouse dorsal horn and around the central canal, corresponding to the areas where nociceptin-like immunoreactive fibres were concentrated. 4. In [35S]-GTPγS membrane assay, nociceptin increased [35S]-GTPγS binding of mouse spinal cord membranes in a concentration-dependent and saturable manner, affording maximal stimulation of 64.1 ± 2.4%. This effect was markedly inhibited by the specific ORL1 receptor antagonist [Phe1ψ (CH2-NH) Gly2] nociceptin (1-13) NH2. None of the μ-, δ-, and κ-opioid and other G-protein-coupled receptor antagonists had a significant effect on basal or nociceptin-stimulated [35S]-GTPγS binding. 5. These findings suggest that nociceptin-containing fibres terminate in the superficial layers of the dorsal horn and the central canal and that nociceptin released in these areas may selectively stimulate the ORL1 receptor to activate G-protein. Furthermore, the unique pattern of G-protein activation in the present study provide additional evidence that nociceptin is distinct from the μ-, δ- or κ-opioid system.

Yes / In this paper, a pyramidal horn antenna loaded with unit cell of metamaterial is proposed, designed and realized for L-band that including terrestrial digital audio broadcasting TDAB, GPS and GSM. The proposed antenna operates in the frequency range from 1.722 GHz to 1.931 GHz. The metamaterial is fabricated on a printed circuit board as Capacitive Loaded Loop (CLL). The work aims to exhibit the advantage of metamaterial loaded inside the horn antenna in terms of the gain enhancement of the radiation pattern and the resonant frequency shift towards lower frequency. The retrieval technique used show that the constitutive parameters of the unit cell as CLL have a zero index metamaterial (ZIM) from 1.34 GHz to 1.49 GHz and a near zero index of refraction from 1.495 GHz to 2 GHz, which is within the operating frequency of the horn antenna. The achieved results show that the total gain is improved over the frequency range. The simulation and the measurement are in good agreement.

The dominant liberal peacebuilding critiques tends to focus on ‘states’ and the failure of interventions in rebuilding them. Consequently, a standardised critique has emerged largely because the critics apply a broad brush across a diverse range of contexts, programmes, issues and activities as illustrated by the lack of scrutiny on mine action and emerging contexts such as Somaliland. The liberal peacebuilding critics critique the standardised ‘one size fits all approach’ employed by interveners, yet they take the same approach. I therefore argue for the need to broaden the critique to include other elements and contexts of peacebuilding. I demonstrate that as an intervention mine action has intrinsic peacebuilding potential. However, the way mine action is implemented both globally and in Somaliland reflects the same dominant characteristics of the liberal peacebuilding critique i.e.; it is externally led; uses technical and standardised formulaic approaches; disregards local context thus failing to secure local ownership. Attributes that the critics argue have led to the failure and/or limited success of peacebuilding interventions. I therefore contend with the critics and demonstrate how these attributes have contributed to the challenges of implementing mine action activities thereby limiting mine actions ‘peace-ability’ potential in Somaliland. However, beyond the implementation modalities there are other factors that further contribute to limiting this potential; these include the Sector Actors; the Somaliland context i.e. the historical and political context, and the perception of Somaliland people. Thus in conclusion I argue for a nuanced critique that acknowledges the challenging realities of implementing programmes in challenging post conflict environments.

Seine Sonate für Horn und Klavier komponierte Matthias Drude (geb. 1960) in Barsinghausen bei Hannover im Jahr 1993 kurz vor seiner Berufung als Dozent für Musiktheorie nach Dresden. Sie steht beispielhaft für eine ganze Reihe von einsätzigen, relativ konzentrierten Kammermusikwerken mit einer Spieldauer von meist weniger als 10 Minuten. Das spieltechnisch anspruchsvolle und emotional eine große Spannbreite zwischen „verträumt“ und „aggressiv“ durchmessende knapp achtminütige Werk nimmt seinen Ausgangspunkt in einer Art D-Dur-Tonalität, die jedoch durch die kleine Sexte B getrübt wird. Innerhalb der Exposition ist ein zweites, rhythmisch mit dem ersten verwandtes Thema u. a. am Lagenwechsel (hoch statt tief) gut zu erkennen. Im weiteren Verlauf verschwimmen die Grenzen von Formteilen. Durchführungsartiges beherrscht die Szene bis zum Schluss. Die Hornpartie ist in der Partitur klingend notiert.

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