In this study, the aim was to investigate the effects of pH on therapeutically important protein, recombinant human growth hormone (rhGH), production by Pichia pastoris considering the expression levels of regulatory genes. In this frame, firstly the host microorganism was selected between two different methanol utilization phenotypes of P. pastoris, Mut+ and MutS on media containing glycerol/methanol or sorbitol/methanol. The highest rhGH production, 120 g L-1, and hGH gene expression, 9.84x109 copies mg-1 CDW, were achieved in the medium containing 30 g L-1 sorbitol and 1% (v/v) methanol by P. pastoris hGH-Mut+ strain. Thereafter, effects of pH on rhGH production and stability were investigated in laboratory scale bioreactors. RhGH was more stable at pH 5.0. Throughout the production, it is seen that medium of pH decreased. Thereafter, effects of pH on rhGH were investigated in pH controlled pilot-scale bioreactor. In addition to rhGH concentration, AOX intracellular enzyme activity, extracellular proteases concentrations / expression levels of hGH, AOX, pep4, prb1 and prc1 genes were determined. The highest cell concentration was obtained as 53 g L-1 at pH 6.0 but hGH concentration was found as 24 mg L-1 at t=24 h. The highest rhGH concentration was obtained as 271 g L-1 with 42 g L-1 cell density at pH 5.0 in medium containing sorbitol at t=24 h. At this condition, the overall product and cell yield on total substrate were found as 2.08 mg g-1 and 0.15 g g-1. Furthermore, the highest expression levels of hGH and AOX were attained at pH 5.0. Moreover, by keeping pH at 5.0, expression levels of three types of vacuolar proteases were minimized.

In this study, the aim was to achieve high concentration of recombinant human growth hormone (rhGH) production by recombinant Pichia pastoris by investigating the effects of various operation parameters and to determine the suitable peptide ligand sequence that shows affinity and specificity to hGH. In this context, firstly the effect of temperature and Tween-20/80 addition on production and cell growth were investigated. While at T=30 and 32&deg / C, there was no difference, at 27 and 25&deg / C cell growth slowed down and production decreased significantly. The addition of Tween-20/80 in existence of co-substrate sorbitol did not affect the bioprocess while in absence of sorbitol Tween alone did not show the same positive effect on product formation and cell growth. Thereafter at T=30&deg / C, without addition of Tween, three sets of pilot scale bioreactor experiments were performed. In the first set, the effect of methanol feeding rate on bioprocess characteristics were investigated at the specific growth rates of &mu / =0.02, 0.03 and 0.04 h-1. While the highest cell concentration was achieved at &mu / =0.04 h-1, the highest rhGH concentration was achieved at &mu / =0.03 h-1. Secondly, conducting methanol feeding at &mu / =0.03 h-1, pH=5.5 experiment was conducted. The highest cell concentration, 45 g L-1 and maximum rhGH concentration 0.25 g L-1 were achieved at t=18 h of the process. Finally, the effect of batch sorbitol feeding on bioprocess was observed by the addition of 50 g L-1 sorbitol at t=0, 14 and 31 h of the production phase. It was shown that sorbitol addition to the medium increased process duration / hence cells enter stationary phase after a longer production phase. However, the protease concentration continued increasing with respect to time and at the end of the process reached twice the concentration it was obtained with single sorbitol addition case decreasing the rhGH concentration. In selection of the peptide sequence that shows affinity towards hGH, phage display method was conducted. Additionally the sequences from literature and computational design were used as alternatives. The interaction between these peptides and hGH was investigated by isothermal titration calorimetry and surface plasmon resonance.

In this study, regulatory gene effects on recombinant human growth hormone (rhGH) production by Bacillus subtilis were investigated. For this purpose, firstly Bacillus strains, which are deficient in abrB, aprE, degQ, degS, degU, scoC, sinI, sinR, and spo0A genes, were selected according to the regulatory gene network of aprE gene (serine alkaline protease gene of B. subtilis) since due to the degQ promoter and the pre-signal sequence of subC gene cloned in front of the hGH gene, hGH is produced by mimicking the serine alkaline protease synthesis. R-Bacillus strains were constructed by transformation of pMK4::pre(subC)::hGH plasmid to the selected strains. Thereafter, by the laboratory scale experiments, strains having the highest hGH production capacity were determined as scoC, aprE, sinR, and degU knockout strains. Using these strains, fermentation experiments were carried out in pilot-scale bioreactor in defined medium. Effect of pH control was also investigated and the highest cell and hGH concentration was obtained by scoC knockout strain in pH controlled operation as 1.62 kg m-3 and 126 g m-3, respectively. By this strain, the overall product and cell yield on total substrate were found as 16.12 g kg-1 and 0.15 g g-1, respectively. Furthermore, the highest total protease activity was attained by degU knockout strain as 65 U cm-3. On the other hand, maximum total organic acid secretion was determined as 1.31 kg m-3 in aprE knockout strain.

In this study, recombinant human growth hormone (rhGH) production by Pichia pastoris-Mut+ strain was improved by designing feeding strategies which were applied in the production phase of the bioreactor operations. During the bio-reactor experiments the cell growth, sorbitol and methanol consumptions, recom-binant hGH production, alcohol oxidase (AOX) activity, the by-products protease and organic acid concentrations were followed and analyzed. In this context, in the first part of the study, three bioreactor operations were designed and per-formed. In general, the designed strategies are fundamentally based on simulta-neous feeding of the two substrates starting at t=0 h of the production phase, i.e., batch-wise 50 gL-1sorbitol feeding, together with fed-batch methanol feeding with a specific growth rate of &mu / 0=0.03 h-1 or &mu / 0=0.04 h-1, and fed-batch sorbitol feeding with a specific growth rate of &mu / 0=0.025h-1 which was calculated based on the specific consumption rate qS=0.152 g g-1h-1 of sorbitol. Consequently, sorbitol concentration was kept constant at 50 gL-1 within t=0-15h of the production phase / where, sorbitol feeding was terminated at t=15h. Amongst, in the first strategy (SSM1), methanol was fed to the system with the specific growth rate of &mu / 0=0.03 h-1, and the H+ concentration (pH) in the bioreactor was kept constant at pH=5.0. In the second strategy (SSM2), pH was kept constant at 5.5 until t=24h of the induction phase (production phase), thereafter, was reduced to pH= 5.0 / where methanol was fed to the bioreactor with the specific growth rate of &mu / 0=0.03 h-1. In the third strategy (SSM3), methanol was fed with the specific growth rate of &mu / 0=0.04 h-1, and the pH in the bioreactor was kept constant at pH 5.0. The highest rhGH production and cell concentration were achieved in the first strategy SSM1 as CrhGH=640 mg L-1 and CX=105.3 g L-1, and the overall cell and product yields on total substrate were calculated as YX/S =0.21 g g-1 and YCrhGH/S =1.83 mg g-1. In the second part of this study the two-substrates sorbitol and methanol were fed simultaneously in a solution compose of 1.37 mol sorbitol and 6.21 mol methanol in 13.88 mol water, which is named as SM. In this strategy (SM), the two-substrate solution was fed to the medium with the specific growth rate of &mu / 0=0.03 h-1 on sorbitol until t=30h / thereafter, only methanol was fed to the bio-reactor with the specific growth rate of &mu / 0=0.03 h-1. The highest cell and rhGH concentrations obtained in SM were, respectively, Cx=104.7 g L-1 and CrhGH=124 mg L-1 / and the overall cell and product yields on the total substrate were calcu-lated as YX/S=0.21 g g-1 and YCrhGH/S=0.39 mg g-1. Although the highest cell con-centration obtained at SM is close to that of the SSM1, the rhGH concentration obtained at SM is 5.2-fold lower than that of the strategy SSM1.

Over the past couple of years there has been rapid progress in the development and design of safe and effective delivery systems for the administration of protein and peptide drugs. The effective delivery of these type of drugs are not always as simple as one may think, due to various inherent characteristics of these compounds. Due to the hydrophilic nature and molecular size of peptide and protein drugs, such as recombinant human growth hormone, they are poorly absorbed across mucosal epithelia, both transcellularly and paracellularly. This problem can be overcome by the inclusion of absorption enhancers in peptide and protein drug formulations but this is not necessarily the best method to follow. This investigation focussed specifically on the evaluation of the ability of the PheroidTM carrier system to transport recombinant human growth hormone across mucosal epithelia especially when administered via the nasal cavity. The PheroidTM delivery system is a patented system consisting of a unique submicron emulsion type formulation. The PheroidTM delivery system, based on PheroidTM technology, will for ease of reading be called Pheroid(s) only throughout the rest of this dissertation. The Pheroid carrier system is a unique microcolloidal drug delivery system. A Pheroid is a stable structure within a novel therapeutic system which can be manipulated in terms of morphology, structure, size and function. Pheroids consist mainly of plant and essential fatty acids and can entrap, transport and deliver pharmacologically active compounds and other useful substances to the desired site of action. The specific objectives of this study can be summarised as follows: a literature study on Pheroid technology; a literature study on chitosan and N-trimethyl chitosan chloride; a literature study on recombinant human growth hormone (somatropin); a literature study on nasal drug administration; formulation of a suitable Pheroid carrier; entrapment of somatropin in the Pheroid carrier, and in vivo evaluation of nasal absorption of somatropin in Sprague-Dawley rats. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2007.

The purpose of the study is to purify human growth hormone from the fermentation broth by affinity chromatography. For this purpose, human growth hormone specific oligonucleotide aptamers are selected among an aptamer library / selected oligonucleotides were synthesized and used as ligands. Effect of pH on ligand-human growth hormone complex formation was investigated and the highest complex formation was obtained at pH= 7.0. Human growth hormone is separated from the fermentation broth with 99.8% purity and 41% overall yield. The equilibrium data obtained was described by Langmuir type isotherm where saturation constant (q0) and affinity constant (K) are calculated as 0.338 mg hGH/&micro / mol aptamer and 0.059 mg hGH/ml, respectively. Further, equilibrium data obtained using aptamer affinity column was described by Langmuir type isotherm where saturation constant (q0) and affinity constant (K) are 0.027 mg hGH/&micro / mol aptamer and 1.543 mg hGH/ml, respectively. It is possible that, selected aptamer can be used for purification of bulk amounts of recombinant human growth hormone by using aptamer affinity chromatography.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-26T12:53:16Z No. of bitstreams: 1 LEONARDO_MATOS_SANTOLIM_ZANETTINI_DIS.pdf: 2920184 bytes, checksum: f65b15c1056e1a9173b56c1789377ba8 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-04-06T16:34:38Z (GMT) No. of bitstreams: 1 LEONARDO_MATOS_SANTOLIM_ZANETTINI_DIS.pdf: 2920184 bytes, checksum: f65b15c1056e1a9173b56c1789377ba8 (MD5) / Made available in DSpace on 2018-05-18T14:04:05Z (GMT). No. of bitstreams: 1 LEONARDO_MATOS_SANTOLIM_ZANETTINI_DIS.pdf: 2920184 bytes, checksum: f65b15c1056e1a9173b56c1789377ba8 (MD5) Previous issue date: 2018-01-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / . / Recombinant Human Growth Hormone (RhGh) has been studied in cases of dental implants, TMJ pathologies and bone fractures. It is able to stimulate bone growth in a dose-dependent manner by direct stimulation of chondrocytes. It stimulates proliferation and differentiation of chondroprogenitor cells, also acts directly on osteoblasts, increasing rates of remodeling and bone formation. In this review of the literature and case report, RhGh will be used in alveolar ridge preservation procedures, with the purpose of evaluating the benefits of this technique in maintaining the postextraction bone site viable, aiming a future rehabilitation with dental implants and prostheses.

Made available in DSpace on 2014-06-11T19:35:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-07-07Bitstream added on 2014-06-13T20:06:39Z : No. of bitstreams: 1 dotto_pp_dr_sjc.pdf: 4285690 bytes, checksum: ecfd6441fbc5f11ca356a3ef11cb7bfc (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O propósito no presente estudo foi verificar a existência da proporção áurea entre algumas medidas cefalométricas laterais de indivíduos com síndrome de Down (SD), bem como, observar se houve variações nas razões avaliadas em diferentes períodos de crescimento de acordo com a curva de crescimento puberal (CCP), e entre os sexos. Para tanto, foram analisadas 52 radiografias cefalométricas laterais e 52 radiografias de mão e punho de indivíduos com SD entre seis e 33 anos de idade. A divisão da amostra foi realizada no programa Curva de Crescimento 1.0 (Radiomemory®). Foram selecionados 16 segmentos craniofaciais, gerando 17 razões. As medidas foram calculadas no programa Radiocef 4.0 (Radiomemory®). O erro intra-examinador foi verificado pelo teste de Análise de Regressão Linear (r >0,95). Os resultados obtidos foram submetidos à Análise de Regressão Linear Múltipla e teste t de Student (5%). Das 17 razões, três apresentaram tendência à proporção áurea em todas as fases tanto para o sexo masculino quanto para o feminino (SAcrS/ N-Ena; S-AcrS/Ena-Enp e N-ASPt/Sf1/-C1MS). A razão NPOMxN/ Or-POMx-Or foi áurea para os dois sexos após o período do pico do surto de crescimento puberal. Em Po-N/Co-Go e Co-Gn/N-POMxN, a tendência foi verificada para o sexo feminino em todas as fases, quanto ao sexo masculino, houve variação na ocorrência durante as fases. Para Co-Gn/Go-Pog, essa tendência foi observada para o sexo masculino no final do crescimento. A razão Co-Go/Sf1/-C1MS apresentou variações na ocorrência para ambos os sexos. O autor concluiu que indivíduos com SD apresentaram algumas medidas do crânio em proporção áurea e que para algumas razões analisadas houve variações na ocorrência com relação às fases de crescimento e diferenças entre os sexos foram observadas. / The aim of the present study was to verify the presence of the golden proportion among some lateral cephalometric measurements of individuals with Down's syndrome (DS), as well as, to observe if there were variations in the appraised ratios at different growth phases in agreement with the Puberal Growth Curve (PGC), and between genders. There were used 52 lateral cephalometric X-rays and 52 hand and wrist X-rays from individuals with DS among six and 33 years old. The sample was divided using the software Curva de Crescimento 1.0 (Radiomemory®). It was selected 16 craniofacial segments, generating 17 ratios. The measurements were calculated by Radiocef 4.0 (Radiomemory®) program. The intra-examiner error was analyzed using the Linear Regression test (r >0,95). The results were analyzed using the Multiple Linear Regression test and the t-Student test (5%). Among the 17 ratios, three presented tendency to the golden proportion in all of the phases as much for males as for females (SAcrS/ N-Ena; S-AcrS/Ena-Enp and N-ASPt/Sf1/-C1MS). The ratio NPOMxN/ Or-POMx-Or was golden for both genders after the pubertal growth spurt. For the ratios Po-N/Co-Go and Co-Gn/N-POMxN, the tendency was verified for females in all of the phases, but for males there was found variation in the occurrence during the phases. For the ratio Co- Gn/Go-Pog, that tendency was observed for males in the final phase of the growth. The ratio Co-Go/Sf1/-C1MS presented variations in the occurrence for both genders. Author concluded that individuals with DS presented some measurements of the skull in golden proportion and variations was found for some analyzed ratios in occurrence regarding the growth phases and differences between genders.

Orientador: Luiz Cesar de Moraes / Banca: Jorge Abrão / Banca: Cláudio Fróes de Freitas / Banca: João Carlos da Rocha / Banca: Horácio Faig Leite / Resumo: O propósito no presente estudo foi verificar a existência da proporção áurea entre algumas medidas cefalométricas laterais de indivíduos com síndrome de Down (SD), bem como, observar se houve variações nas razões avaliadas em diferentes períodos de crescimento de acordo com a curva de crescimento puberal (CCP), e entre os sexos. Para tanto, foram analisadas 52 radiografias cefalométricas laterais e 52 radiografias de mão e punho de indivíduos com SD entre seis e 33 anos de idade. A divisão da amostra foi realizada no programa Curva de Crescimento 1.0 (Radiomemory®). Foram selecionados 16 segmentos craniofaciais, gerando 17 razões. As medidas foram calculadas no programa Radiocef 4.0 (Radiomemory®). O erro intra-examinador foi verificado pelo teste de Análise de Regressão Linear (r >0,95). Os resultados obtidos foram submetidos à Análise de Regressão Linear Múltipla e teste t de Student (5%). Das 17 razões, três apresentaram tendência à proporção áurea em todas as fases tanto para o sexo masculino quanto para o feminino (SAcrS/ N-Ena; S-AcrS/Ena-Enp e N-ASPt/Sf1/-C1MS). A razão NPOMxN/ Or-POMx-Or foi áurea para os dois sexos após o período do pico do surto de crescimento puberal. Em Po-N/Co-Go e Co-Gn/N-POMxN, a tendência foi verificada para o sexo feminino em todas as fases, quanto ao sexo masculino, houve variação na ocorrência durante as fases. Para Co-Gn/Go-Pog, essa tendência foi observada para o sexo masculino no final do crescimento. A razão Co-Go/Sf1/-C1MS apresentou variações na ocorrência para ambos os sexos. O autor concluiu que indivíduos com SD apresentaram algumas medidas do crânio em proporção áurea e que para algumas razões analisadas houve variações na ocorrência com relação às fases de crescimento e diferenças entre os sexos foram observadas. / Abstract: The aim of the present study was to verify the presence of the golden proportion among some lateral cephalometric measurements of individuals with Down's syndrome (DS), as well as, to observe if there were variations in the appraised ratios at different growth phases in agreement with the Puberal Growth Curve (PGC), and between genders. There were used 52 lateral cephalometric X-rays and 52 hand and wrist X-rays from individuals with DS among six and 33 years old. The sample was divided using the software Curva de Crescimento 1.0 (Radiomemory®). It was selected 16 craniofacial segments, generating 17 ratios. The measurements were calculated by Radiocef 4.0 (Radiomemory®) program. The intra-examiner error was analyzed using the Linear Regression test (r >0,95). The results were analyzed using the Multiple Linear Regression test and the t-Student test (5%). Among the 17 ratios, three presented tendency to the golden proportion in all of the phases as much for males as for females (SAcrS/ N-Ena; S-AcrS/Ena-Enp and N-ASPt/Sf1/-C1MS). The ratio NPOMxN/ Or-POMx-Or was golden for both genders after the pubertal growth spurt. For the ratios Po-N/Co-Go and Co-Gn/N-POMxN, the tendency was verified for females in all of the phases, but for males there was found variation in the occurrence during the phases. For the ratio Co- Gn/Go-Pog, that tendency was observed for males in the final phase of the growth. The ratio Co-Go/Sf1/-C1MS presented variations in the occurrence for both genders. Author concluded that individuals with DS presented some measurements of the skull in golden proportion and variations was found for some analyzed ratios in occurrence regarding the growth phases and differences between genders. / Doutor

CORTEZ, S.A.E. O efeito do trabalho infantil na estatura final de adultos jovens e características de sua escolaridade - Estudo da coorte de nascidos vivos entre 1978/79, nos hospitais de Ribeirão Preto, SP. 2005. 158 f. Tese (Doutorado) Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 2005. Justificativa: Efeitos negativos a saúde da criança têm sido associados ao trabalho infantil. Entretanto, seus efeitos sobre o desenvolvimento humano ainda precisam ser melhor avaliados em razão do pequeno número de estudos existentes da deficiência de controle de fatores socioeconômicos. Objetivo: avaliar o efeito do trabalho infantil na estatura final e algumas características da escolaridade de jovens entre 22-25 anos pertencentes à coorte de nascidos vivos de parto único hospitalar de Ribeirão Preto, SP - 1978/79. Métodos: O presente estudo incluiu 2063 jovens, pertencentes a coorte inicial de 6827 nascidos vivos na cidade de Ribeirão Preto, Brasil (1978/79). Foram seguidos 30,2% dos homens e 33,5% das mulheres. O trabalho infantil foi classificado de acordo com a idade de início do primeiro trabalho: < 14, 14-16 e 17+ anos. Determinantes da altura foram considerados variáveis de confusão na análise, ao nascer (restrição do crescimento intra-uterino RCIU; comprimento ao nascer, idade materna, escolaridade materna, hábito de fumar na gravidez e ocupação do chefe de família) e na vida adulta (cor/etnia, número de irmãos e atividade física). Para as mulheres, idade da menarca também foi considerada. Análise de regressão linear múltipla estratificada por sexo foi realizada após análise bivariada. As variáveis foram selecionadas para o modelo utilizando-se o procedimento passo a passo com seleção retrógrada das variáveis. Interações plausíveis foram testadas. Análise seqüencial dos fatores associados à altura final também foi realizada, separadamente por sexo. Resultados: A altura média foi 176,0 cm (IC 95% 175,6 176,4) para homens e 162,7 cm (IC 95% 162,3 - 163,0) para mulheres. Trabalho antes dos 14 anos de idade foi observado em 20,4% dos homens e 12,4% das mulheres e trabalho entre 14 16 anos foi observado em 41,7 % dos homens e 36,7% das mulheres. Na análise bivariada o trabalho infantil foi associado à mais baixa estatura final tanto para homens (p= 0.007) quanto para mulheres (p=0.004). No entanto, esta associação não se manteve após o controle pelos fatores de confusão. A análise seqüencial sugere que tanto para os meninos como para as meninas as variáveis sócio econômicas interferiram na associação do trabalho infantil e altura final. Ter trabalhado na infância resultou em pior resultado escolar comparados com quem não trabalhou na infância. Aqueles que iniciaram no trabalho antes dos 14 anos e que não estudavam no momento da pesquisa, 62,3% pararam seus estudos no ensino médio sendo que 30,2% pararam de estudar antes de completar 9 anos de estudo. Para quem iniciou o trabalho entre 14-17 anos, 66% chegaram ao ensino médio e 16,4% tem escolaridade acima de 12 anos. Conclusão: Os resultados deste estudo não corroboram com a hipótese de associação independente entre trabalho infantil e altura final. Este resultado se explica pela ação dos confundidores sociais. Observou-se também que não ter trabalhado na infância propicia um melhor resultado escolar, sendo observado que 60,2 % dos que iniciaram atividade laborativa após 17 anos tem escolaridade alta (acima de 12 anos). / CORTEZ, S.A.E. The effects of child labor on the final height and schooling level of a 22-25 years old population-based-cohort from Ribeirão Preto, Brazil. 2005. 158 f. Thesis (Doctoral) Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 2005. Background: Several adverse health effects have been associated with child labor. However, its effects on human growth are still debatable mostly because of the poor study designs and lack of control of socioeconomic factors. Aim: to assess the effects of child labor on the final height and schooling level of a 22-25-yr old population-based-cohort from Ribeirão Preto, State of São Paulo, located in one of the wealthiest areas in Brazil.Methods: The study included 2063 singletons (30,2% males and 33,5% females), from the initial cohort of 6827 live births in the city of Ribeirão Preto, Brazil (1978/79). Height was measured in centimeters at the follow-up examination. Child labor was defined according to the age at the first job (< 14; 14-16; 17yrs+). Known determinants of height and possible confounders were considered in the analysis, at birth (intrauterine growth restriction IUGR, length at birth, mothers age and education, maternal smoking habit, and socioeconomic position), and at adult age (color/ethnicity, number of siblings, physical activity). For females, age at menarche was also considered as confounder. Statistical analysis was performed separately for males and females. Bivariate and stratified analysis was followed by multivariate linear regression analysis. Variables were selected into the model using a backward stepwise-like selection. Plausible interactions were tested and residual analysis was performed. Moreover, sequential analysis of confounder factors associated to the final height was performed. Results: Mean height was 176.0 cm (95% CI 175.6-176.4) for males and 162.7 cm (95% CI 162.3 163.0) for females. Labor before 14 yrs of age was observed in 20.4% of males and 12.4% of females, and labor between 14 and 16 yrs of age was observed for 41.7% of males and 36.7% of females. In the bivariate analysis child labor was statistically associated with lower height for both males (p=0.0065) and females (p=0.007). However, this association did not remain significant after adjusting for confounders. The sequential analysis suggested that for both males and females socioeconomic factors were the most important factors. Regarding schooling achievement, among those who worked before 14 years old and were not studying at the moment of the study, 62,3% finished or withdraw the studies at any moment during the high school the 30.2% before completing 9 years of study. High school profile was similar for those who begin to work between 14 and 17 years old and 16.4% achieved more than 12 years old of study. Conclusion: our study did not support the association between child labor and final height. Moreover, it was observed that those children who did not work early in life achieved higher schooling levels.