Human β-catenin is a dual-functioned protein responsible for regulating cell-cell adhesion and gene transcription. To activate gene transcription, β-catenin must be shuttled into the nucleus where it interacts with various co-activators to activates gene transcription. Various studies have identified proteins that bind to specific amino acid sequences in β-catenin for proper gene transcription regulation. Compared to the single beta-catenin in most animals, C. elegans surprisingly contains four β-catenins. Though structurally similar, these beta-catenins became distinct during nematode evolution, resulting in four β-catenins that differ in functions. SYS-1 is one such β-catenin that loses its adhesion ability and is specialized in activating transcription of genes in the nucleus. Across different animals, β-catenin shares similar amino acid sequences and structure. SYS-1, while it shares the similar structure to other β-catenins, is the most divergent C. elegans beta-catenin when comparing amino acid sequences. In addition, while SYS-1 interacts with homologs of proteins that bind to and regulate human β-catenin, the binding sites of those proteins to SYS-1 is unknown. Here, we identify novel sites for beta-catenin’s gene transcription role within SYS-1 that greatly differed from human β-catenin. We also identify a novel mechanism for beta-catenin nuclear import, which is still largely unknown in any system, by identifying a candidate importer that associates with SYS-1 is required for SYS-1 dependent cell fate. In summary, though SYS-1 has a well-conserved function dictating cell fate in response to developmental signals, it has evolved novel regulatory, functional and localization mechanisms and therefore serves as a model for the plasticity nuclear importer that helps shuttle SYS-1 into the nucleus identified specific regions in SYS-1 that is involved in activating transcription which will result in cell fate changes.

Medical imaging can involve different modalities, such as computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and ultrasound. Each examination generated by these modalities has a set of unique instructions, the imaging protocol, which are imaging parameters determine the signal and contrast for creating a particular medical image. Properly managing imaging protocols is like systemically documenting an instruction handbook, which guarantees the quality of scans by providing the radiologist and technicians with appropriate procedures for a given indication. It also ensures patients’ safety by reducing repeated scans to acquire the desired image information. Radiology Protocols (RP) is a company that provides an online medical protocol database to improve protocol management. It recently developed RP Import, an imaging protocol import software, to automatically collecting elements from the medical imaging file – Digital Imaging and Communications in Medicine (DICOM) files, and mapping them to the specified protocol database. With the help of RP Import, protocol creation is much faster and eliminates manual definition of the parameters, thus this tool lays the foundation of the further development of imaging protocol management. Radiology Protocols also developed a series of Modality Calculators to assist radiologists and technicians to build or modify imaging protocols as they are needed. These calculators cover most of the essential medical parameter calculations associated with different modalities. By using them, computing specific parameters while editing protocols becomes more convenient, and determining the use and amount of certain medical parameters becomes more precise as well. In summary, RP Import and Modality Calculators are two meaningful tools in protocol management, and they also play very important roles in regulating procedure and dosage during the medical image practice.

<p>Distance is of great influence when deciding whom to trade with. This thesis examines how the importance of distance in international trade has evolved. This is done using an extended generalized gravity model, which includes population, real exchange rate and a dummy variable for membership in the European Union. Using data for the EU27 and the four largest economies in the world outside of EU, this model estimates the effect of distance on trade from 1980 to 2005. This thesis shows that the impact of distance has evolved towards having a greater negative effect on trade during the observed years. The reason for this could be a development towards regional trade, at the expense of long-distance trade.</p>

<p>In the beginning of 1990s Sweden implemented several measures in order to maintain price stability. These measures have resulted in an environment in which inflation is lower and more stable. The same development could be seen in other OECD countries. At the same time a decrease in exchange rate pass-through was noticed in many countries. This has led researchers to believe that there may be a connection, between these two phenomena. This dissertation analyzes whether there has been any change in exchange rate pass-through for manufacturing products in Sweden between the high inflation period (1977-1993) and the low inflation period (1994-2006). The result shows that there is a difference in the exchange rate pass-through between the two periods. During the low inflation period the degree of pass-through was lower than during the high inflation period.</p>

The chloroplast is the green organelle in the plant cell responsible for harvesting energy from sunlight and converting it into sugars and ATP. Origins of this organelle can be traced back to an endosymbiotic event in which a primitive eukaryotic cell capable of oxidative phosphorylation engulfed a free-living cyanobacterium capable of photosynthetic respiration (1). Immediately following this event the details are not clear, however what is known is that over the course of evolution, the engulfed cyanobacteria relinquished approximately 97% of its protein coding sequences to the host cell nucleus, thus making the newly formed chloroplast reliant on its host cell (2). This resulted in the requirement of a post-translational import mechanism (3,4). Accomplishing posttranslational import are Translocons of the Outer and Inner Chloroplast membranes, or TOC and TIC complexes (5). These complexes are comprised of multiple proteins whose function is the efficient and robust recognition of chloroplast-destined preproteins and their subsequent import. Preproteins are synthesized in the cytosol with a cleavable Nterminal extension of approximately 50-150 amino acids known as a transit peptide (6-8). It is the transit peptide that is recognized by the Toc complex which facilitates the import of the preprotein (9). It is this transit peptide mediated chloroplast protein import mechanism that will be the subject of this dissertation. Presented in Chapter II is an analysis of the basal enzymology of the isolated, soluble forms of the Toc GTPases. Chapter III analyzes the homo- and heterodimeric interaction between Toc proteins and how this oligomerization can be modulated. Chapter IV presents evidence that the transit 2 peptide interacts with the Toc proteins in such a way as to increase enzymatic activity as well as bias the dimeric equilibria. Analysis of the data presented in Chapters II, III and IV allow the creation of a chloroplast protein import model, Chapter V, to potentially explain the observed phenomenon. Finally, Chapter VI presents potential future directions for this research.

During chloroplast formation via endosymbiosis most of the plastid genome was transferred to the host nuclear genome. Genomic and proteomic analysis suggests that >95% of the original plastid proteome is now encoded in the nucleus, and these now cytosolically fabricated proteins require a post-translational transport pathway back into the organelle. This process is not well understood, yet it has been shown to involve translocons at the outer and inner envelope of the chloroplast membranes (TOC & TIC). These translocons interact with a cleavable N-terminal extension of between 20 and 100 residues on chloroplast-bound precursor proteins known as the transit-peptide. Precursor proteins pass through the outer membrane via the outer chloroplast membrane beta-barrel, Toc75. In addition to containing a transmembrane β-barrel, Toc75 also contains three polypeptide transport (POTRA) domain repeats at the N-terminus. Despite widespread occurrence the role of POTRAs is poorly understood. One possibility is that they function to promote either homo- or heterotypic protein:protein interactions. To investigate these possibilities, we modeled the psToc75 POTRA domains and purified recombinant POTRA domains. POTRA1, POTRA3, and POTRA1-3 have been used to investigate interactions. Homotypic POTRA interactions have been supported by crosslinking experiments and analytical ultra centrifugation (AUC). Crosslinking data shows POTRA1 and POTRA3 undergo oligimerization. AUC suggests that POTRA1 may homodimerize. Heterotypic interactions have been studied via pull-down assays, crosslinking, and AUC and demonstrate that POTRA1 and POTRA3 interact with transit peptide. Soluble POTRA1-3 seems to stimulate precursor protein import into isolated chloroplasts in an import assay. The role of POTRAs in guiding TOC assembly by homodimerization is being investigated, and experiments to establish how POTRAs aggregate are underway.

European Union’s (EU) dependence on fossil fuel imports strongly affects its energy supply and economic and national stability. In order to reduce its dependence and maintain the leadership position in GHG emissions reductions and renewable energy consumption, EU has set ambitious targets of reducing GHG emissions by at least 20% compared with 1990, raising the share of renewable energy to 20% and increasing the levels of biofuels in transport fuels to 10% by 2020. While some of the countries, due to their large renewable potential, have already reached set targets, some are still far behind. Biomass, with 69.8% share in gross inland consumption of renewables, has the greatest potential. Since some EU members have low availability of biomass, and are scarce in other renewable sources, they have become biomass importers. As some studies have shown, imports of biomass reduce cost of achieving targets for renewable electricity and increase electricity production from biomass. Wood pellets, as currently the most tradable solid biomass commodity, already reached significant shares in imports and consumption of biomass in some EU countries. The most of pellets were traditionally imported from Canada; however, last year US became the EU’s largest importing partner with 534,000 tons of industrial pellets exported mainly to Belgium and Netherlands. The EU’s increasing demand for wood pellets was a major driver for substantial increase in the wood pellet production capacity in US, with many plants being constructed for export to EU. This thesis evaluates the possibility of producing wood and switchgrass pellets in East Tennessee, assuming three feedstock scenarios, and their export to EU. Results from the base-case model showed that production costs of pellets were $155, $164 and $170 per ton, while price of pellets on the EU market will have to be $207, $216 and $222 per ton in the 100% mill residue, 40/60 blend and 100% switchgrass scenarios, respectively, for the project to breakeven. Sensitivity analysis showed a strong impact of feedstock price, moisture and exchange ratio on project’s return on investment.

<p>The study draws on secondary data from the Bureau of Statistics in Lesotho. Simple and multiple linear regression models techniques are used to analyze the relationship between the GDP of Lesotho and the GDP of manufacturing. The secondary data is analyzed using Statistical Packages for Social Sciences (SPSS) and Excel. The major finding is that there exists a strong positive linear relationship ( r = 0.986) between the GDP of Lesotho and the GDP of manufacturing. This means that every time the GDP of manufacturing increases the GDP of Lesotho does the same. Based on this finding, the study recommends that in order to improve, sustain and maintain the economic growth and to avoid further deterioration in the manufacturing industry, the manufacturing capacity must be strengthened for it to effectively deal with growing competition and rapid economic changes.</p>

This thesis investigates the determinants of Taiwan’s import changes and the underlying factors of the decreasing Swedish export to Taiwan between 1994 and 2005. The empirical study includes 36 countries from both the Pacific Rim and OECD. Based on a modified gravity model of trade, the regression model aims to examine how GDP growth in the exporting country, exchange rate changes, common language, and membership in APEC affect Taiwan’s import volume. The result shows estimates with expected signs, with 49.8 percent of the vari-ance in Taiwan’s changed import volume explained by the exogenous variables. Exchange rate change and language are statistically significant. Data on commodity groups of importance for Sweden and Taiwan are also ex-amined, and they show that Taiwan has changed its import demand and has started to import goods other than those Sweden in previous years strongly exported to Taiwan. Taiwan’s regional trading partners have also gained export shares at the expense of Swedish exports. The results are in line with theory and it will be hard for Sweden in the future to compete with the increasing regional trade of East Asia where common lan-guage and culture are of big importance.

This is unique study of import is of the greatest importance, since no studies of import across regions have earlier been performed. Import is a driving force of innovations and therefore most important for a stable growth. This master thesis is about interregional import, as well as the strong spatial concentration of imports in the Swedish system of network. Five hypothesises are presented in the last section of chapter two. The variables to be used in the analysis are then divided into two main groups, before empirically tested in different combinations of regression models. The main conclusion of this thesis is a significant correlation between import, and the two independent variables export and firm R&amp;D, and its result goes in line with the theoretical framework of this thesis, regional specialisation in import and export nodes