No description available.

No description available.

The Juan de Fuca Ridge, located 400 km off the coast of Washington State, is home to unstable and unpredictable hydrothermal vent sites where chemosynthetic communities flourish. In 2007 the manned submersible ALVIN retrieved a Ridgeia piscesae tubeworm community in its entirety from the side of the Tower sulfide edifice from the Endeavor segment of the Juan de Fuca Ridge (47 55.416720 N, 129 6.487020 W, at a depth of 2269 m) using the Bushmaster Jr. collection device. The collection was analyzed for community structure and the data collected were compared to that from several other hydrothermal vent communities. It was determined that substrate composition is a factor that heavily influences community structure. The data were then compared to the community succession model developed by Sarrazin et. al. in 1997 and 1999 (Sarrazin et. al. 1997, Sarrazin and Juniper 1999). The Tower community was found to expand the model as a new community succession classification; that of community iii low flow. The Tower community was then analyzed for diversity, structure and tubeworm morphology in conjunction with two other communities from differing substrata. The Ridgeia piscesae tubeworms were found to be of the "long skinny" morphotype, one that was previously thought to only reside on basaltic substrate. The Tower community has similar species richness and higher species evenness than those from basaltic substrate, but similar richness and lower evenness that those from sulfide. This community type combines the characteristics of those from both substrata, resulting in a community with diversity and structure that is an intermediary between sulfide and basaltic substrates. / Biology

Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (p. 103-112).

The hydrothermal vent environment, in its extreme spatial and temporal variability, offers the opportunity to study habitats that are naturally fragmented and unstable. The vestimentiferan tubeworm Ridgeia piscesae is a foundation species inhabiting hydrothermal vent habitat in the Northeast Pacific Ocean. R. piscesae is a phenotypically plastic species and is arranged in a metapopulation spatial structure, with each local population displaying one of a range of morphotypes. Ridgeia piscesae participates in an obligate symbiosis that is dependent on hydrogen sulphide in the hydrothermal vent fluid that supplies each local population. Hydrothermal fluid flow is highly variable in the hydrothermal vent environment and hydrogen sulphide flux is a limiting nutrient for R. piscesae; this variability may create differences in habitat quality. The objective of this study is to determine whether local populations of R. piscesae centered on high and low flux hydrothermal fluid outputs are similar in body condition, reproductive condition, and juvenile recruitment. Using the submersibles ROPOS and Alvin, I collected high flux and low flux sample pairs from within meters of each other at multiple sample sites on Axial Seamount and the Endeavour segment of the Juan de Fuca Ridge. I used morphological measurements, histology and lipid analysis to assess physiological and reproductive condition. I also determined the relative abundances of new and older recruits in high and low flux local populations. I found that low flux habitat was inferior in its ability to support Ridgeia piscesae at all stages in the tubeworm’s life cycle. In terms of body condition, local populations in low flux habitat had lower body weight, greater body length, smaller anterior tube diameter, lower trophosome volume, lower total lipid volume, and lower branchial plume condition. With respect to reproductive condition, local populations in low flux habitat had lower proportions of reproductive individuals, less sperm transfer, lower gonad volume, and fewer mature oocytes; there was no difference in sperm development stages between high and low flux habitat. From the perspective of the individual, low flux tubeworms live longer, and lifetime reproductive output may be comparable to high flux tubeworms. However, turnover is higher in the high flux habitat, so reproductive output of high flux populations is greater than that of low flux populations. Juvenile recruitment was biased toward high flux habitat, although this trend was not significant and recruitment to low flux habitat was still notable. The differences between reproductive output and juvenile recruitment between these habitats support a source-sink model of population dynamics. From the perspective of the metapopulation, low flux habitat is inferior in its ability to support Ridgeia piscesae at all stages in the tubeworm’s life cycle. This distribution of relative contributions to the overall population of a key species in a Marine Protected Area (MPA) should factor into management decisions affecting MPA boundaries and use. / Graduate

Prokaryotic microorganisms, which are at the base of deep-sea hydrothermal vent food webs, adapt rapidly to environmental fluctuations. This study aimed at comparing bacterial communities in contrasting hydrothermal habitats to better understand compositional adaptations to local conditions. I first used small subunit (SSU) ribosomal RNA (rRNA) gene sequences to compare mat-forming bacterial communities associated with iron oxides at two hydrothermal vent sites on the Tonga Arc, southwest Pacific. Operational taxonomic units (OTUs), defined at 97% sequence similarity, were affiliated to a great diversity of autotrophic and heterotrophic groups. Metabolically diverse Gammaproteobacteria dominated the sample from Volcano 19, collected at 992 m depth. The sample from Volcano 1, collected at 197 m depth, was dominated by iron-oxidizing bacteria from the class Zetaproteobacteria. The depth of the sampling sites was proposed to explain clone library dissimilarities. In the following studies, I compared bacterial communities associated with the vestimentiferan tubeworm Ridgeia piscesae, a foundation species at the Juan de Fuca Ridge. Samples of the polychaete were collected from tubeworm habitats in contrasting flow regimes that influenced temperature and hydrogen sulphide concentrations. Free-living bacteria were analyzed using both sequencing and 454 pyrosequencing of the SSU rRNA gene. Statistical analyses suggested a predictable pattern of bacterial community composition for the two habitats, with higher proportions of sulphur and hydrogen oxidizers in High Flow and more heterotrophic groups in Low Flow environments. Temperature, available energy for metabolism, and stability of the habitat were suggested to explain these distinctive bacterial communities. Symbiotic assemblages were investigated using the same sequencing methods together with catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Gammaproteobacteria dominated all sequence libraries, followed by Epsilonproteobacteria. CARD-FISH confirmed the co-occurrence of these groups within R. piscesae trophosomes. Statistical analyses indicated distinctive membership and structure of trophosome assemblages between sampling sites. Analysis of R. piscesae juvenile showed distinctive structural properties when compared to adult individuals, but similar membership, within sampling sites. These results suggested that the composition of trophosome assemblages might be affected by specific physical and chemical conditions at each vent site and that a selection process might occur during R. piscesae’s development. / Graduate / 0410 / 0416 / 0329 / nathalieforget@gmail.com

No description available.

No description available.

At the northern extent of the Cascadia subduction zone, the subducting Explorer and Juan de Fuca plates interact across a translational deformation zone, known as the Nootka fault zone. The Seafloor Earthquake Array Japan-Canada Cascadia Experiment (SeaJade) was designed to study this region. In two parts (SeaJade I and II, deployed from July – September 2010 and January – September 2014), seismic data from the SeaJade project has led to several important discoveries. Hypocenter distributions from SeaJade I and II indicate primary and secondary conjugate faults within the Nootka fault zone. Converted phase analysis and jointly determined seismic tomography with double-difference relocated hypocenters provide evidence to several velocity-contrasting interfaces seaward of the Cascadia subduction front at depths of ~4-6 km, ~6-9 km, ~11-14 km, and ~14-18 km, which have been interpreted as the top of the oceanic crust, upper/lower crust boundary, oceanic Moho, and the base of the highly fractured and seawater/mineral enriched veins within oceanic mantle. During SeaJade II, a MW 6.4 mainshock and subsequent aftershocks, known as the Nootka Sequence, highlighted a previously unidentified fault within the subducted Explorer plate. This fault reflects the geometry of the subducting plate, showing downward bending of the plate toward the northwest. This plate bend can be attributed to negative buoyancy from margin parallel mantle flow induced by intraslab tearing further northwest. Seismic tomography reinforces the conclusions drawn from the Nootka Sequence hypocenter distribution. Earthquakes from the entire SeaJade II catalogue reveal possible rotated paleo-faults, identifying the former extent of the Nootka fault zone from ~3.5 Ma. / Graduate

Intra-archipelago waterways, including tidal strait networks, present a complex set of barriers to, and conduits for sediment transport between marine basins. Tidal straits may also be the least well understood tide-dominated sedimentary environment. To address these issues, currents, sediment transport pathways, and seabed sedimentology & geomorphology were studied in the central Salish Sea (Gulf and San Juan Islands region) of British Columbia, Canada and Washington State, USA. A variety of data types were integrated: 3D & 2D tidal models, multibeam bathymetry & backscatter, seabed video, grab samples, cores and seismic reflection. This dissertation included the first regional sediment transport modelling study of the central Salish Sea. Lagrangian particle dispersal simulations were driven by 2D tidal hydrodynamics (~59-days). It was found that flood-tide dominance through narrow intra-archipelago connecting straits resulted in the transfer of sediment into the inland Strait of Georgia, an apparent sediment sink. The formative/maintenance processes at a variety of seabed landforms, including a banner bank with giant dunes, were explained with modelled tides and sediment transport. Deglacial history and modern lateral sedimentological and morphological transitions were also considered. Based on this modern environment, adjustments to the tidal strait facies model were identified. In addition, erosion and deposition patterns across the banner bank (dune complex) were monitored with 8-repeat multibeam sonar surveys (~10 years). With these data, spatially variable bathymetric change detection techniques were explored: A) a cell-by-cell probabilistic depth uncertainty-based threshold (t-test); and B) coherent clusters of change pixels identified with the local Moran's Ii spatial autocorrelation statistic. Uncertainty about volumetric change is a considerable challenge in seabed change research, compared to terrestrial studies. Consideration of volumetric change confidence intervals tempers interpretations and communicates metadata. Techniques A & B may both be used to restrict volumetric change calculations in area, to exclude low relative bathymetric change signal areas. / Graduate / 2018-12-07