Esta tese é parte integrante do trabalho de doutorado em Performance Musical com especialização em Regência Coral do Programa de Pós-graduação em Música da Escola de Comunicações e Artes da Universidade de São Paulo. Durante os quatro anos de doutorado foram realizados concertos, ensaios e atividades práticas desenvolvidas simultaneamente à escrita deste texto que discute questões referentes à prática de um performer. Nosso objeto central é a relação entre a escrita musical e a interpretação. Buscamos compreender, como intérpretes, quais são os espaços criativos e seus limites dentro de uma obra musical. Para isso, utilizamos a obra coral do compositor brasileiro Osvaldo Lacerda, conhecido por sua escrita rigorosa e bastante indicativa. Após uma reflexão sobre os conflitos existentes entre composição e interpretação, fizemos uma análise geral da escrita composicional de Lacerda buscando encontrar os espaços para a criatividade do intérprete. Em seguida, analisamos com profundidade quatro composições de Osvaldo Lacerda: Automação, Romaria, A Primeira Missa e o Papagaio e o movimento Uníssono da obra Quatro Estudos para Coro. As obras analisadas foram performatizadas durante o período de doutorado junto ao Coro de Câmara Comunicantus e pudemos formar, experimentar e consolidar nossas concepções interpretativas, as quais comentamos e justificamos ao longo do texto. Por fim, apontamos para algumas respostas sobre o espaço criativo na performance de uma obra extremamente indicativa e sobre a responsabilidade do intérprete diante da partitura. / This thesis is part of the doctoral work in Musical Performance with specialization in Choral Conducting for the Post-Graduation Program in Music of the Escola de Comunicações e Artes da Universidade de São Paulo. During the four doctoral years, concerts, rehearsals and practices were undertaken simultaneously with the writing of this text which discusses questions regarding the practice of a performer. Our main object is the relationship between musical writing and performance. As interpreters, we seek to understand what the creative spaces and their limits within a work of music are. In order to do this, the choral work of the Brazilian composer Osvaldo Lacerda - known for his rigorous and rather indicative writing - was used. After a reflection on the conflicts between composition and interpretation, a general critical analysis of the compositional writing of Lacerda was carried out to identify the spaces for the creativity of the interpreter. Next, an in-depth analysis was carried out on four compositions by Osvaldo Lacerda: Automação, Romaria, and O Papagaio and the movement Uníssono of the work Quatro Estudos para Coro. The analyzed works were presented during the doctoral period together with the Chamber Choir Comunicantus and we could form, test and consolidate our interpretative conceptualizations, as is commented and justified throughout the text. Lastly, we point to some answers regarding the creative space in the performance of an extremely indicative piece and about the responsibility of the interpreter to the sheet music.

I examined the hypothesis that high variability in Sea Surface Temperature (SST) and ocean color are associated with higher coral cover and slower rates of decline of coral cover within the Florida Keys National Marine Sanctuary (FKNMS). Synoptic SST time series maps, covering the period 1994-2005, were constructed for the FKNMS with data collected using the National Oceanic and Atmospheric Administration (NOAA) Advanced Very High Resolution Radiometer (AVHRR) satellite sensors. The SST data were compared with coral cover time series assessments at 36 sites conducted by the Coral Reef and Evaluation Monitoring Program (CREMP; 1996-2005), sponsored by the Environmental Protection Agency and the State of Florida. Out of the 36 stations, Smith Shoals routinely experienced very different and extreme environmental conditions relative to the rest of the stations, including extreme salinity, suspended sediments, and "black water" events that led to the death of coral reef organisms such as in 2002. Among the other 35 stations, sites that experienced moderately higher SST variability (mean variance > 6) relative to other sites showed a trend toward higher percentage coral cover (r=0.62, p=6.33x10-5, N=35) and relatively slower rates of decline (r=0.41, p=0.02, N=35) over the 12-year study period. The results suggest that coral reefs sites that are continuously exposed to high but not extreme variability in temperature may develop resilience against episodes of extreme cold or elevated SST. Variability of suspended sediments and water clarity were estimated using satellite-derived, normalized water-leaving radiance products. Ocean color data were obtained from the Sea-viewing Wide-Field-of View Sensor (Sea WiFS) from 1998 to 2005. Normalized water-leaving radiance at 443 (Lwn443) was used as a proxy to examine variability in water clarity, and normalized water-leaving radiance at 670 (Lwn670) was used as a proxy to study variability in suspended sediments. A weak relationship was identified between variability of Lwn443 and Lwn670 and coral cover as estimated by CREMP assessments in 2005 (r=0.43, p = 0.01, N=35 and r = 0.47, p = 0.005, N=35, respectively). There was a weak relationship between coral cover change and Lwn670 from 1988 to 2005 (r = 0.46, p = 0.05, N=35), but there no relationship was observed between variability of Lwn443 and change in coral cover (r =0.27, p =0.11, N=35). Further research is required to understand the origin, concentration and composition of dissolved or suspended materials that change the turbidity of waters around reefs of the FKNMS, and whether these changes can be adequately interpreted by examining concurrent satellite imagery. Ultimately, such remote sensing and field research is required to understand how water quality affects the health of coral reefs, and how coral ecosystems adapt to environmental variability.

Corals, the foundation species of tropical reefs, are in rapid global decline as a result of anthropogenic disturbance. On many reefs, losses of coral have coincided with the over-harvesting of reef herbivores, resulting in ecosystem phase-shifts from coral to macroalgal dominance. It is hypothesized that abundant macroalgae inhibit coral recovery and recruitment, thereby generating ecological feedback processes that reinforce phase-shifts to macroalgae and further diminish reef function. Notwithstanding, the extent to which macroalgae directly outcompete coral, the mechanisms involved, and the species-specificity of algal-coral competition remains debated. Moreover the capacity for herbivores to prevent vs. reverse ecosystem phase-shifts to macroalgae and the roles of herbivore diversity in such phenomena remain poorly understood. Here I demonstrate with a series of field experiments in the tropical Pacific and Caribbean Sea that multiple macroalgae common to degraded reefs directly outcompete coral using chemical warfare, that these interactions are mediated by hydrophobic secondary metabolites transferred from algal to coral surfaces by direct contact, and that the outcomes of these allelopathic interactions are highly species-specific. Using field observations and experiments in the tropical Pacific, I also demonstrate that the process of herbivory attenuates the competitive effects of allelopathic algae on corals by controlling succession of algal communities, and that the herbivore species responsible for macroalgal removal possess complementary tolerances to the diversity of chemical defenses deployed among algae, creating an essential role for herbivore diversity in reversing ecosystem phase-shifts to macroalgae. Lastly, I demonstrate with field experiments in the tropical Pacific that algal-coral competition simultaneously induces allelochemicals and suppresses anti-herbivore deterrents in some algae, likely due to trade-offs in the productions of defense metabolites with differing ecological functions. Together, these studies provide strong evidence that chemically mediated competitive and consumer-prey interactions play principal roles in coral reef degradation and recovery, and should provide resource managers with vital information needed for effective management of these ecologically and economically important but threatened ecosystems.

Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1984. / Bibliography: leaves [259]-275. / Photocopy. / Microfilm. / xvii, 275 leaves, bound ill., maps 29 cm

Coral reefs are one of the most productive, diverse and complex ecosystems on Earth. They are very important ecologically, economically and socially, but are subject to increasing deleterious disturbances. To protect coral reefs and manage the sustainable use of their resources it is necessary to understand how coral communities respond to disturbances and to use this understanding to project the likely ecological trajectories of disturbed coral reefs in spatial and temporal contexts. Three powerful tools exist to address this issue: (1) in situ monitoring that describes ecological transitions of coral communities at very fine spatial scale; (2) time-series of maps derived from high spatial resolution remote sensing images that provide multi-temporal synoptic views of the reefs; and (3) spatially- and temporally-explicit models that are able to handle ecosystems complexity and represent their spatial dynamics. The combination of these three tools to map and monitor coral communities remained to be addressed. This dissertation developed an integrative approach to characterise, map and model coral communities’ responses to disturbances. This approach provides a basis for monitoring coral reefs at temporal and spatial scales matched to disturbance impacts and coral reefs patchiness. This was achieved by investigating the dynamics of three different Indo-Pacific reefs and by following four steps: - Developing and applying a method to characterise how detailed coral communities can be mapped before and after a major cyclone event from a short time-series of high spatial resolution images (IKONOS, Quickbird) on Aboré Reef (New-Caledonia); - Using the methods developed in the first step to assess whether decadal-scale coral dynamics can be retraced and monitored from time-series of aerial photographs and satellite images spanning at least 30 years on Saint-Leu (Réunion Island) and Heron (Australia) Reefs; - Developing a spatially- and temporally-explicit model of coral communities’ dynamics with cellular agent-based formalism on the western section of Heron reef flat; and - Assessing the relevance of the mapping, monitoring and modelling tools developed in this work, into an integrated approach for coral reef monitoring. For the first step, accurate monitoring requires that descriptions of the reef features are coherent with the local scale of disturbance impacts in space and time. While such a monitoring paradigm is applied in terrestrial environments, it is not the case for coral reefs. A before-after cyclone time-series of satellite images from Aboré Reef was used to test this paradigm on coral reefs. In situ data provided a new three-level hierarchical coral community typology (45 classes at the finest level). Photo-interpretation and hierarchical mapping methods were applied to an IKONOS image and a Quickbird image taken before and after cyclone Erica respectively. Application of this paradigm yielded a highly detailed multi-temporal maps of pre- and post-cyclone coral communities and recommendations to design reef-scale monitoring protocols. For the second step, the temporal scale of monitoring projects needs also to match the inherent reef dynamics. To assess the applicability of this temporal component of the paradigm at a decadal scale, the hierarchical mapping approaches developed for Aboré Reef were applied to a 33-year time-series of satellite images (two Quickbird images) and airborne photographs (five scanned images) of Saint-Leu Reef. The mapping approach overcame challenges due to different images qualities and to the lack of in situ observations in time and space before cyclone Firinga in 1989. This demonstrated the potential for further applications of the approach in reef monitoring protocols based on complementary in situ and remote sensing data to help understand the dynamics of reef-top coral reef communities and geomorphology over years to decades. In the next step, the modelling component of this work focused on a proof-of-concept for spatially-explicit modelling of coral growth by simulating maps of reef flat colonisation on a 16 686 m2 section of Heron Reef. To do this a 35-year time-series of two satellite Quickbird pan-sharpened images and five aerial photographs of Heron Reef was first used to hierarchically map and quantify the areal expansion of coral on the reef flat. The coral growth was driven by several artificially induced local sea-level rises associated with engineering works on the reef flat. Vertical and horizontal growth rates were quantified in terms of percentage of the total area colonised each year by corals. Coral community maps and coral growth rates estimated from the image time-series were used to constrain an accretive cellular growth model. Although only preliminary the model produced coral growth likelihood maps corresponding to observed fine-scale coral growth patterns. This suggested the tool had promise for further applications in reef management. This dissertation developed an integrative approach to characterise, map and model coral communities’ responses to disturbances, providing a basis for monitoring coral reefs at ecological, temporal, and spatial scales matching the patchiness of the communities’ distribution and disturbance impacts. The contributions of the work to the applied fields of coral reef mapping, modelling and monitoring were demonstrated through the results achieved and the development of protocols that do not require specialized image processing algorithms and methods. This opens perspectives for further development of the approach on other coral reefs around the world.

Orientador: Adriana Giarola Kayama / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Artes / Made available in DSpace on 2018-08-13T11:36:23Z (GMT). No. of bitstreams: 1 Fernandes_AngeloJose_D.pdf: 15120528 bytes, checksum: 34f7101f7688b07174d8d6f268fdceb2 (MD5) Previous issue date: 2009 / Resumo: Com o objetivo de prover material de pesquisa capaz de auxiliar o regente coral em sua função de preparador vocal, o presente trabalho é um amplo estudo sobre técnica vocal e práticas interpretativas no âmbito da música coral. Trata-se de um trabalho direcionado a regentes que atuam à frente de coros mistos adultos, de natureza amadora e formação de câmara cujo número de cantores varia de 16 a 45 vozes. Após uma reflexão introdutória, são apresentadas características históricas, técnicas e estilísticas de diversos estilos de música vocal e coral. Entre esses estilos estão a Renascença, o Barroco, o Classicismo, o Romantismo, a música do século XX e, por fim, a música coral brasileira do período colonial à atualidade. Em cada um dos estilos são abordados aspectos como a qualidade sonora das vozes, as técnicas de produção vocal, a natureza da prática coral, bem como sugestões para a construção da sonoridade desses vários estilos nos dias atuais. Na sequência, partindo de uma reflexão sobre a função do regente de preparador vocal, são definidos e descritos diferentes aspectos técnicos envolvidos na construção da sonoridade coral. Entre esses aspectos estão a administração da respiração, ressonância vocal, dicção, registração vocal, timbre, vibrato, homogeneidade, equilíbrio, afinação e precisão rítmica. Em seguida, é apresentado, como estudo de caso, um relato sobre a elaboração e a aplicação de um programa experimental de preparo vocal para coros com o Madrigal Musicanto de Itajubá, coro regido pelo autor deste trabalho. Por fim, como conclusão de toda a pesquisa realizada, apresenta-se uma linha metodológica para o preparo vocal de cantores corais que aborda aspectos como postura, respiração, ataque vocal, ressonância, dicção, registração vocal, legato, staccato, agilidade vocal, extensão vocal, dinâmica, homogeneidade e afinação / Abstract: With the objective of providing useful research material for choral conductors who are also responsible for the vocal preparation of their singers, the present work is an ample study on vocal technique and performance practice in choral music. It is a work for choral conductors of mixed chamber choirs of adult amateurs singers whose number of members varies from 16 to 45 voices. After introductory considerations, historical, technical and stylistic peculiarities of various styles of vocal and choral music are presented. Among these styles are the Renaissance, the Baroque, the Classicism, the Romanticism, the Modernism, and finally, the Brazilian choral music from the Colonial period to the present time. For each of these styles, aspects like tone color, vocal techniques, the essence of choral practice as well as suggestions for sonority building are approached. In the sequence, based on the role of the conductor as a vocal coach, different technical aspects related to the construction of the choral tone - breath management, vocal resonance, diction, vocal registration, timbre, vibrato, blend, balance, intonation, and rhythmic precision - are defined and described. Afterwards, the author presents a case study that describes the elaboration and application of an experimental program of vocal preparation with the Madrigal Musicanto of Itajubá, a choir conducted by this author. Finally, to conclude this research, methodological guidelines for the vocal preparation of choral singers - dealing with the various technical aspects discussed in this work - are presented / Doutorado / Praticas Interpretativas / Doutor em Música

No description available.

This dissertation investigates social and ecological factors that facilitate effective management of coral reefs as social-ecological systems. Meta-analytical and field-based methods were employed to examine current management challenges and identify strategies that improve management effectiveness and coral reef health. A meta-analysis was used to evaluate biological indicators of reef health in relation to the types of fishing regulations in place (no-take areas, gear restriction areas, and periodic closures) and the actor groups (community-based, co-management, state, private) involved in management efforts for coral reef fisheries throughout the world. Other than enhancement of fish biomass within no-take areas that was significantly greater than in gear restriction areas, most biological indicators benefitted similarly from management techniques of no-take areas and gear restriction areas. Community-based and co-management were the best performing management arrangements for some biological outcomes but require further case studies to verify findings. Investigation of management effects by region indicated that previously degraded reefs received fewer benefits from management implementation than did relatively healthier reefs. For field investigations, the Comoros islands in the Western Indian Ocean served as a model for tropical coral reefs with challenging socioeconomic contexts, high biodiversity, and high vulnerability to coral reef degradation. Empirical study at 21 sites was used to identify the relative effects of natural and anthropogenic threats to coral reefs of the Comoros. Most previous studies of reef health focus on primarily natural factors or a single anthropogenic threat. This study examined suites of natural factors and human activities to identify the relative importance of each on reef health. Human activities including fishing, sand extraction, and beachfront housing and development were the best predictors of reef health status. Most notably, human population and fishing predicted fish richness, abundance, and biomass with seasonal variation in the effects, while site orientation strongly predicted benthic cover. Field studies in the Comoros were also used to investigate the roles of community and state actors in co-management and compare effectiveness of comanagement across sites with varying levels of actor participation. Effective management was found to occur with community or `meta-community' (in this case, a Marine Protected Area in which the efforts of several communities were organized) participation in governance and support of state or external agents, while resilient management that overcame considerable challenges was found to occur only with strong community participation and leadership in governance. External agents were found to contribute to development of meta-community governance structure and initiation of community participation through education and capacity building. The findings from these studies reveal that coral reef management can be improved through context appropriate regulations that address detrimental human activities and through wide acceptance and participation in governance with cooperation among states, communities, and external agents.

The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to -neurotoxins (-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main -NTx of M. fulvius venom. -NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%-78%; radiochemical purity 92%; and stability at 48 h 85%. The median lethal dose (LD50) and PLA(2) activity of bioconjugated -NTx decreased 3 and 2.5 times, respectively, in comparison with native -NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of -NTx-DTPA-Ga-67 in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with Ga-67-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of -NTx-DTPA-Ga-67 remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of -NTx-DTPA-Ga-67. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.

Environmental variation is ubiquitous in natural systems. The genetic and physiological mechanisms governing population-level responses to this variation will impact the process of speciation and the capacity for populations to persist in a changing climate. Until recently, population-level responses to environmental selection remained largely unexplored in marine systems due to the historical assumption that the inherently dispersive nature of most marine taxa would preclude their ability to specialize to local environments. This dissertation represents the first investigation of population-level responses to environmental variation in a Caribbean reef-building coral. This research integrates ecological, physiological, genetic and genomic methods to (1) determine patterns of local adaptation in the Florida Keys, (2) identify stressors driving adaptive responses, (3) distinguish the physiological and genetic mechanisms underlying coral adaptation and (4) assess the potential for future adaptation in the common reef-building coral Porites astreoides. Results demonstrate that corals adapt and/or acclimatize to their local habitat and that this specialization incurs fitness costs. Temperature differences between reefs likely play a selective role in differentiating inshore and offshore coral populations. Genetic and gene expression differences indicate that coral hosts play a substantial role in driving these population-level differences. Inshore corals exhibit greater gene expression plasticity, which may be involved in stabilizing physiological responses to temperature fluctuations experienced at inshore reefs. In addition, naïve juvenile coral recruits from inshore reefs exhibit a growth rate advantage over offshore recruits under elevated temperature treatment, suggesting that thermotolerance differences observed in adult populations could continue to evolve in response to climate change. Taken together these results provide novel insight into the drivers of reef decline in the Florida Keys and the role of the host in coral adaptation capacity. / text