No Brasil, o amplo incentivo e a disponibilidade de financiamento à produção habitacional de baixa renda, nos últimos anos têm estimulado a padronização do produto e aplicação de conceitos de produção em massa neste setor. No entanto, a entrega de produtos altamente padronizados a clientes com diferentes necessidades, desconsiderando seus modos de vida e percepções de valor, frequentemente resulta em produtos inadequados, os quais necessitam ser modificados logo após a entrega. A customização em massa tem se destacado como uma estratégia para aprimorar a geração de valor e aumentar o grau de satisfação de clientes na indústria da manufatura. No contexto da habitação de baixa renda, a definição de um conjunto de opções relevantes do ponto de vista dos clientes, baseado na sua percepção de valor, é um dos principais desafios para a implementação desta estratégica. O objetivo da presente pesquisa consiste em propor um método para identificar atributos customizáveis da empreendimentos habitacionais customizados, baseado em um modelo conceitual denominado cadeia meios-fim. Este modelo foi adaptado ao contexto de habitação de baixa renda, de forma a ser utilizado para relacionar os atributos do produto com os valores dos clientes, por meio da aplicação da técnica laddering. Além disso, a pesquisa tem como objetivo específico desenvolver dispositivos visuais que apoiem a tomada de decisão sobre a oferta de espaços de solução para unidades habitacionais customizadas. Com o objetivo de testar a aplicabilidade da solução desenvolvida, foram realizados dois estudos empíricos. O primeiro estudo foi desenvolvido para o segmento de mercado específico de uma empresa construtora de habitações, enquanto no segundo estudo fez-se a comparação de quatro segmentos de mercado delineados no Programa Minha Casa Minha Vida. A principal contribuição do método proposto é indicar as unidades de customização que são mais relevantes para os clientes finais e modelar a geração de valor na perspectiva dos usuários finais, de forma a entender as prioridades estabelecidas pelos mesmos. Além disso, os resultados permitem entender diferenças sobre a geração de valor para diferentes perfis de clientes e identificar oportunidades de melhoria em produtos habitacionais existentes. / In Brazil, high incentives and availability of funding for low-cost housing projects, in recent years, have encouraged product standardization and the application of mass production ideas in that sector. However, the delivery of highly standardized housing units to customers with different requirements, without considering their lifestyles and perceptions of value, often results in inadequate products, which need to be modified soon after delivery. Mass customization has been pointed out as an effective strategy to improve value generation and increase the degree of client satisfaction in the manufacturing industry. In the context of low-cost housing, the definition of a set of relevant options from the point of view of clients, based on their perceptions of value, is a major challenge for the implementation of this strategy. The aim of this research work is to propose a method for identifying value-adding attributes in customized housing projects, based on a conceptual model named means-end chain. That model was adapted to the low-cost housing context in order to connect product attributes to clients’ values, by applying the laddering technique. Besides, this investigation has the secondary aim of devising visual devices that can support decision-making related to the solution space of customised housing units. Two empirical studies were carried out in order to assess the applicability of the proposed solution. The first study was developed considering the specific market segment of a house-building company, while in the second study a comparison was made between four segments of the My House My Life Program. The main contribution of the proposed method is to identify which customization units are the most relevant ones for the final clients, as well as to model value generation from the point of view of clients, with the aim of understanding the priorities established by them. Moreover, the results explain differences in value generation for distinct client profiles, and identify improvement opportunities for existing housing products.

No Brasil, o amplo incentivo e a disponibilidade de financiamento à produção habitacional de baixa renda, nos últimos anos têm estimulado a padronização do produto e aplicação de conceitos de produção em massa neste setor. No entanto, a entrega de produtos altamente padronizados a clientes com diferentes necessidades, desconsiderando seus modos de vida e percepções de valor, frequentemente resulta em produtos inadequados, os quais necessitam ser modificados logo após a entrega. A customização em massa tem se destacado como uma estratégia para aprimorar a geração de valor e aumentar o grau de satisfação de clientes na indústria da manufatura. No contexto da habitação de baixa renda, a definição de um conjunto de opções relevantes do ponto de vista dos clientes, baseado na sua percepção de valor, é um dos principais desafios para a implementação desta estratégica. O objetivo da presente pesquisa consiste em propor um método para identificar atributos customizáveis da empreendimentos habitacionais customizados, baseado em um modelo conceitual denominado cadeia meios-fim. Este modelo foi adaptado ao contexto de habitação de baixa renda, de forma a ser utilizado para relacionar os atributos do produto com os valores dos clientes, por meio da aplicação da técnica laddering. Além disso, a pesquisa tem como objetivo específico desenvolver dispositivos visuais que apoiem a tomada de decisão sobre a oferta de espaços de solução para unidades habitacionais customizadas. Com o objetivo de testar a aplicabilidade da solução desenvolvida, foram realizados dois estudos empíricos. O primeiro estudo foi desenvolvido para o segmento de mercado específico de uma empresa construtora de habitações, enquanto no segundo estudo fez-se a comparação de quatro segmentos de mercado delineados no Programa Minha Casa Minha Vida. A principal contribuição do método proposto é indicar as unidades de customização que são mais relevantes para os clientes finais e modelar a geração de valor na perspectiva dos usuários finais, de forma a entender as prioridades estabelecidas pelos mesmos. Além disso, os resultados permitem entender diferenças sobre a geração de valor para diferentes perfis de clientes e identificar oportunidades de melhoria em produtos habitacionais existentes. / In Brazil, high incentives and availability of funding for low-cost housing projects, in recent years, have encouraged product standardization and the application of mass production ideas in that sector. However, the delivery of highly standardized housing units to customers with different requirements, without considering their lifestyles and perceptions of value, often results in inadequate products, which need to be modified soon after delivery. Mass customization has been pointed out as an effective strategy to improve value generation and increase the degree of client satisfaction in the manufacturing industry. In the context of low-cost housing, the definition of a set of relevant options from the point of view of clients, based on their perceptions of value, is a major challenge for the implementation of this strategy. The aim of this research work is to propose a method for identifying value-adding attributes in customized housing projects, based on a conceptual model named means-end chain. That model was adapted to the low-cost housing context in order to connect product attributes to clients’ values, by applying the laddering technique. Besides, this investigation has the secondary aim of devising visual devices that can support decision-making related to the solution space of customised housing units. Two empirical studies were carried out in order to assess the applicability of the proposed solution. The first study was developed considering the specific market segment of a house-building company, while in the second study a comparison was made between four segments of the My House My Life Program. The main contribution of the proposed method is to identify which customization units are the most relevant ones for the final clients, as well as to model value generation from the point of view of clients, with the aim of understanding the priorities established by them. Moreover, the results explain differences in value generation for distinct client profiles, and identify improvement opportunities for existing housing products.

This study aims at understanding the decision to buy "Made in Mozambique" products, which is important for developing marketing strategy and promoting national products. To explore the factors influencing consumers' buying decisions, means-end theory and its associated methodology provide a useful framework for understanding why people buy "Made in Mozambique" rice by characterising the relationships between the attributes, consequences associated with those attributes, and the values those consequences help reinforce. Methodology: a total of 20 consumers were interviewed. It is important to mention that only rice was used in this study. The laddering interviewing technique was then used to link these chains of meanings. Analyses and interpretation of the resulting data provide an effective complement for studying consumer purchasing motives and suggest recommendations for managerial practice and future research efforts. Summary of the study findings include: (i) Attributes: Quality, cost, national; (ii) Consequences: healthy, well-being, affordable, trust, contribution, and fulfillment; and (iii) Values: Feeling happy, longevity and self-esteem.The theoretical component of this research covers the definition and discussion of Means-end theory and consumer decision-making. / Industrial Psychology / M.A. (Industrial & Organisational Psychology)

La toxine stable à la chaleur de type b (STb) est une des toxines produites par les souches Enterotoxigenic Escherichia coli (ETEC) impliquée dans le développement de la diarrhée. Une étude antérieure par Goncalves et al. (2009) a démontré que les cellules ayant internalisé la toxine STb démontraient une morphologie qui rappelle l’apoptose. Le changement du potentiel membranaire observé par Goncalves et al. (2009) nous a incité à vérifier la capacité de la toxine STb à induire l’apoptose des cellules HRT-18 et IEC-18 par la voie intrinsèque. Les cellules HRT-18 et IEC-18 ont été traitées avec de la toxine purifiée pour une durée de 24 heures puis ells ont été récoltées et examinées pour des caratéristiques de l’apoptose. L’activation des caspases-9 et -3, mais pas de la caspase-8, a été observée dans les deux lignées cellulaires à l’aide des substrats fluorescents spécifiques pour chaque caspase. L’ADN extrait des cellules HRT-18 et IEC-18 a révélé une fragmentation lorsque migré sur gel d’agarose. La condensation et la fragmentation des noyaux ont été observées en microscopie à fluorescence suite à une coloration de l’ADN au Hoechst 33342. Les indices apoptotiques des cellules HRT-18 et IEC-18 traitées avec des quantités croissantes de STb montrent une dose-réponse pour les deux lignées. L’activation de la caspase-9 est une indication que la voie intrinsèque de l’apoptose est activée dans les cellules HRT-18 et IEC-18. L’absence de l’activation de la caspase-8 démontre que la voie extrinsèque n’est pas impliquée dans la mort cellulaire médiée par STb. / Heat-stable toxin b (STb) is one of the toxins produced by Enterotoxigenic Escherichia coli (ETEC) strains implicated in the development of diarrhea. A previous study conducted by Goncalves et al. (2009) showed that cells having internalized STb toxin demonstrated apoptotic-like morphology. The change in the mitochondrial membrane potential observed by Goncalves et al. (2009) prompted us to verify the ability of STb toxin to induce apoptosis via the intrinsic pathway in HRT-18 and IEC-18 cells. Both cell lines were treated with purified STb toxin for a period of 24 hours, harvested, and examined for apoptotic features. Activation of caspases-9 and -3, but not -8, was observed in HRT-18 and IEC-18 cells as determined with the use of fluorescent substrates specific to each caspase. Extracted DNA revealed DNA laddering when migrated on agarose gels. Nuclear condensation and fragmentation of Hoechst 33342 stained DNA of HRT-18 and IEC-18 cells were visualized by fluorescence microscopy. Apoptotic indexes of HRT-18 and IEC-18 cells treated with increasing amounts of STb toxin revealed dose-dependent responses in both cell lines. The activation of caspase-9 is an indication of the intrinsic pathway being activated in HRT-18 and IEC-18 cells by STb toxin. The lack of caspase-8 activation demonstrates that the extrinsic pathway of apoptosis is not involved in the programmed cell death mediated by STb.

Submitted by Fabricia Fialho Reginato (fabriciar) on 2015-06-17T01:20:25Z No. of bitstreams: 1 0000017C.pdf: 14426451 bytes, checksum: d858dd3df16bdda77e391da36241864d (MD5) / Made available in DSpace on 2015-06-17T01:20:25Z (GMT). No. of bitstreams: 1 0000017C.pdf: 14426451 bytes, checksum: d858dd3df16bdda77e391da36241864d (MD5) Previous issue date: 2012-08-27 / Nenhuma / Este trabalho dedicou-se a investigar a natureza do espaço urbano a partir da ótica do design estratégico. Com essa proposta, o design passa a ser considerado com foco no território, mais especificamente sobre a cidade e o seu contexto dentro de uma área conurbada e complexa que configura as regiões metropolitanas. Este estudo tem como objeto específico a cidade de Canoas, localizada na região metropolitana de Porto Alegre, e parte da discussão dos fundamentos e conceitos que caracterizam os espaços territoriais urbanos, a partir de um resgate teórico com base em autores que referenciam esse tema. No segundo momento, traz-se à tona as bases conceituais do design estratégico e a contextualização do território, a partir da sua ótica. Assim, o território passa a ser visto, e experienciado, como um bem de uso, que dá base à aplicação de dois métodos de coleta de dados, um qualitativo e outro quantitativo, permitindo uma imersão no universo complexo de percepções e visões do conjunto de atores que integram o espaço social de Canoas. A análise e interpretação desse conjunto de dados permite que se construa uma base de referencias para a efetivação de uma etapa de experiência no qual, através de um workshop de design, são criados cenários futuros para a cidade. / This work had dedicated to investigate the nature of urban spaces from the perspective of strategic design. With this proposal, the design becomes to be considered with a focus on the territory, specifically about the city and its context within an complex and conurbation area that sets the metropolitan areas, having the city of Canoas as the specific object of this study, a city located in the metropolitan area Porto Alegre. This work of the discussion of the fundamentals and concepts that characterize the territories and urban spaces, from a theoretical basis of the authors that reference and theme. After this, is brought to discussion, the conceptual foundations of strategic design and the context of the territory from its perspective. Thus, the territory is seen and described as a commodity, and, under this condition, is developed the application of two metology for data collection, a qualitative and another one quantitative method allowing an immersion in the complex universe of perceptions and views of the set of actors who are part of the social universe of Canoas. The Analysis and interpretation of this data set allows to build a reference base for the realization of a stage of experimentation in which, through a design workshop, future scenarios are created for the city.

La toxine stable à la chaleur de type b (STb) est une des toxines produites par les souches Enterotoxigenic Escherichia coli (ETEC) impliquée dans le développement de la diarrhée. Une étude antérieure par Goncalves et al. (2009) a démontré que les cellules ayant internalisé la toxine STb démontraient une morphologie qui rappelle l’apoptose. Le changement du potentiel membranaire observé par Goncalves et al. (2009) nous a incité à vérifier la capacité de la toxine STb à induire l’apoptose des cellules HRT-18 et IEC-18 par la voie intrinsèque. Les cellules HRT-18 et IEC-18 ont été traitées avec de la toxine purifiée pour une durée de 24 heures puis ells ont été récoltées et examinées pour des caratéristiques de l’apoptose. L’activation des caspases-9 et -3, mais pas de la caspase-8, a été observée dans les deux lignées cellulaires à l’aide des substrats fluorescents spécifiques pour chaque caspase. L’ADN extrait des cellules HRT-18 et IEC-18 a révélé une fragmentation lorsque migré sur gel d’agarose. La condensation et la fragmentation des noyaux ont été observées en microscopie à fluorescence suite à une coloration de l’ADN au Hoechst 33342. Les indices apoptotiques des cellules HRT-18 et IEC-18 traitées avec des quantités croissantes de STb montrent une dose-réponse pour les deux lignées. L’activation de la caspase-9 est une indication que la voie intrinsèque de l’apoptose est activée dans les cellules HRT-18 et IEC-18. L’absence de l’activation de la caspase-8 démontre que la voie extrinsèque n’est pas impliquée dans la mort cellulaire médiée par STb. / Heat-stable toxin b (STb) is one of the toxins produced by Enterotoxigenic Escherichia coli (ETEC) strains implicated in the development of diarrhea. A previous study conducted by Goncalves et al. (2009) showed that cells having internalized STb toxin demonstrated apoptotic-like morphology. The change in the mitochondrial membrane potential observed by Goncalves et al. (2009) prompted us to verify the ability of STb toxin to induce apoptosis via the intrinsic pathway in HRT-18 and IEC-18 cells. Both cell lines were treated with purified STb toxin for a period of 24 hours, harvested, and examined for apoptotic features. Activation of caspases-9 and -3, but not -8, was observed in HRT-18 and IEC-18 cells as determined with the use of fluorescent substrates specific to each caspase. Extracted DNA revealed DNA laddering when migrated on agarose gels. Nuclear condensation and fragmentation of Hoechst 33342 stained DNA of HRT-18 and IEC-18 cells were visualized by fluorescence microscopy. Apoptotic indexes of HRT-18 and IEC-18 cells treated with increasing amounts of STb toxin revealed dose-dependent responses in both cell lines. The activation of caspase-9 is an indication of the intrinsic pathway being activated in HRT-18 and IEC-18 cells by STb toxin. The lack of caspase-8 activation demonstrates that the extrinsic pathway of apoptosis is not involved in the programmed cell death mediated by STb.

This study aims at understanding the decision to buy "Made in Mozambique" products, which is important for developing marketing strategy and promoting national products. To explore the factors influencing consumers' buying decisions, means-end theory and its associated methodology provide a useful framework for understanding why people buy "Made in Mozambique" rice by characterising the relationships between the attributes, consequences associated with those attributes, and the values those consequences help reinforce. Methodology: a total of 20 consumers were interviewed. It is important to mention that only rice was used in this study. The laddering interviewing technique was then used to link these chains of meanings. Analyses and interpretation of the resulting data provide an effective complement for studying consumer purchasing motives and suggest recommendations for managerial practice and future research efforts. Summary of the study findings include: (i) Attributes: Quality, cost, national; (ii) Consequences: healthy, well-being, affordable, trust, contribution, and fulfillment; and (iii) Values: Feeling happy, longevity and self-esteem.The theoretical component of this research covers the definition and discussion of Means-end theory and consumer decision-making. / Industrial Psychology / M.A. (Industrial & Organisational Psychology)

During the past years, wooden one and two family houses showed a higher growth rate on the German construction market compared to conventional material like brick and stone. Wood as construction material became more and more attractive. Furthermore, a growing demand on multifamily houses could be recognized, yet, wood has not been considered as an alternative in this field of application until now. Trying to understand the end-users attitudes towards wooden multifamily houses, this licentiate thesis aims at exploring German end-users’ perceptions of wooden multifamily houses. To collect the necessary data, the Association Pattern Technique (APT) was applied, which is a two-stage method and has its foundations in the Means-End theory. In the first stage, 31 in-depth laddering interviews were carried out, which served as the base for the matrix-survey in the second stage, where 229 answers were received. Extending the existing method in the matrix-survey stage allowed for important additional data to be uncovered. The results show that individuals associate certain product attributes with wooden multifamily houses and that these attributes are thought to entail specific consequences. These consequences are in turn linked with the personal values of the respondents. The most dominant perceptual orientations of different groups of respondents are shown and significant differences could be found in how individuals in German perceive wooden multifamily houses depending on age, income and habitation.

Value is fundamental in business-to-business marketing, where marketing revolves around the term value. Understanding the value of offering through customers' eyes is important for business success. Due to the subjective nature of value, it's imperative to understand the value based on the customer's individual interpretation. In this context, perceived value plays a central theme for this study, and analyzed in the terms of benefits and sacrifices. But to capture the broader picture of customers' value functional, social, and emotional perceived values were considered. The research questions were formed to investigate the kind of values that customers perceive, and another question was framed to investigate the multidimensionality of values. In order to answer the research questions, a qualitative descriptive approach was applied and through judgmental sampling, respondents were chosen. The five potential customers were interviewed through semi-structured technique. The authors applied the model “value hierarchy” to investigate the customer's perceived value which consists of three layers: attributes, consequences and objects; it shows correlation among the three layers. The laddering technique was used in connection with hierarchy, through this technique authors understood how consumers were able to perceive value in each layer. The tangibilization strategy was used to provide cues that helped build mental construct of services in customer’s minds. Customers assess the services by evaluating its value. The value from the consequences were perceived multidimensionally by customers depending on the attributes. Customers perceived functional value in aspects of saving time and ease of workload. They were able to perceive value socially, when some features helped manage stress among the employees and through provision of these services to end customers saw an increase in firm awareness in the market. Customers also felt a feeling of happiness when using certain attributes especially among the end customers thereby bringing emotional value into the picture. All the different values perceived helped reach their respective end-goals.  The model found useful to investigate the customer value sequentially, also evaluating current customer’s needs. The study revealed that the attributes aren't chosen without a purpose. Customers perceive some benefits from the attributes that help achieve their specific needs. The study was confirmation about integrating the model with different value dimensions helped determine the connection of these dimensions with customer value. The study ends with presenting managerial, theoretical and practical implications and provides ground for future research in exploring other framework of values, followed by limitations of the study.

Previously it has been shown that cultures of Plasmodium falciparum died following exposure to a febrile temperature of 40°C, as demonstrated by a decrease in parasitemia of the following generation. In the current study, the effect of 40°C treatment on culture media, erythrocytes, and parasite glucose consumption, were ruled out as possible influences on parasite death, demonstrating that 40°C impacted the parasites directly. Metabolic profiling of DNA synthesis, protein synthesis, and glucose utilization during exposure to 40°C clearly indicated that febrile temperatures had direct effect on major metabolic pathways and parasite development, beginning 20-24 hr after erythrocyte invasion. The ring stages were relatively refractory to heat and recovered completely if returned to 37°C. The mechanism of parasite death was investigated for evidence of an apoptosis-like pathway in cells treated with 40°C, chloroquine, and staurosporine. Lack of typical physiological hallmarks, namely, caspase activation, characteristic mitochondrial membrane potential changes, and DNA degradation as indicated by DNA laddering, eliminated ‘classical’, apoptosis as a mechanism of parasite death. Parasites dying under the influence of 40°C, staurosporine, and chloroquine initially appeared pyknotic in light and electron microscopy, as in apoptosis, but eventual swelling and lysis of the food vacuole membrane led to secondary necrosis. Initially, chloroquine did induce DNA laddering, but it was later attributed to occult white blood cell contamination. While not apoptosis, the results do not rule out other forms of temperature-induced programmed cell death.