Insect-human interactions are very complex; one example is the relationship between humans and the multicolored Asian lady beetle (ALB; Harmonia axyridis). ALB, a native to Asia, was introduced into North American agricultural fields and orchards as a biological control agent for aphids and other soft-bodied insects. However, it is considered a pest by some humans as it invades houses in the fall and winter months. Additionally, ALBs feed on fruits when aphids are scarce, and the hemolymph secreted by ALBs contaminates fruit and affects the taste of fruit products. ALBs invasive behavior has led to concern that this non-native species may be outcompeting native lady beetle species, perhaps leading toward the latter’s extinction. Our study aims to provide genomic and proteomic framework for further study and management of ALB. Insect genomes represent gateways into their complex physiological, behavioral, and structural characteristics, and consequently can be leveraged in the development of highly targeted strategies for the control of pests, the propagation/protection of beneficial species, and responsible stewardship of insect biodiversity. For this study, ALBs were collected on the Mississippi State University campus in Starkville, MS, and their DNA was sequenced using Illumina and Oxford Nanopore technologies. The data was assembled and annotated using multiple computational biology techniques. Over 40K protein-coding genes were predicted with high confidence from the ALB sequence assembly. To complement the genome assembly, the proteome of ALB was explored using LC-MSMS analysis and 2-D electrophoresis. Annotation was used to characterize and identify the proteins found in the proteomic analysis of ALB. Before completing our study, a Japanese H. axyridis genome assembly was published by the Beijing Institute of Science. The Japanese ALB genome had a higher assembly quality than ours, but it was not annotated. We annotated both the Japanese and Mississippi ALB genomes. Comparative analyses were performed to identify possible variations that could have resulted from adaptation, but the data revealed no significant differences between Japanese and Mississippi assemblies. Overall, the results from our study, including the annotation of the Japanese ALB assembly, provide a better understanding of the biology of the ALB and inform further research aimed at managing interactions of this species with humans, their crops, and the environment.

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Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2014-10-20T12:27:08Z No. of bitstreams: 2 Dissertacao Rafael Mendonca Lisita Pinto - 2014.pdf: 3410801 bytes, checksum: 568a59b5ffe00961f389baa657ee1671 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-10-20T17:34:24Z (GMT) No. of bitstreams: 2 Dissertacao Rafael Mendonca Lisita Pinto - 2014.pdf: 3410801 bytes, checksum: 568a59b5ffe00961f389baa657ee1671 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-10-20T17:34:24Z (GMT). No. of bitstreams: 2 Dissertacao Rafael Mendonca Lisita Pinto - 2014.pdf: 3410801 bytes, checksum: 568a59b5ffe00961f389baa657ee1671 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-04-23 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This dissertation results of the research developed throughout the Master’s Degree course within the Art and Visual Culture Graduate Program in FAV-UFG. It aims to investigate de representation of uncanny identities in music videos, starting out with some analysis of Lady Gaga’s videos, in order to develop my videographic discourse in my music video “Electric Guitar”. The main goal is to continue creating subversive audiovisuals, which consist in primary and complex products that contemplate several parameters of approach, exploring sensorial matters and establishing bonds with historical, sociological and cultural issues. The first chapter presents Lady Gaga and her artistic body of work which, curiously, defends minorities in spite of the singer being inserted into the star system. The second chapter introduces concept of “the uncanny”, from teoretical dialogues and, also, addresses their presence in music videos. The third chapter focuses on the study of music video as a audiovusal format, its relevance in visual culture and how they develop through media. The forth chapter reapproaches Lady Gaga’s music videos, selected according to this investigation theme, focusing on their analysis. The last chapter discusses the creative experience of “Electric Guitar” through my goal of reassuring the relevance of studying the uncanny and, also, my attempt to expand music video’s frontiers by employing new perspectives to them, without escaping the plastic aesthetics predominant in pop. / Esta dissertação é resultante do projeto de pesquisa realizado através do Programa de Pós-graduação em Arte e Cultura Visual, nível mestrado, da FAV-UFG, e visa apresentar o tema da representação de identidades estranhas em videoclipes, partindo de uma investigação no campo, acompanhada de análises dos clipes da artista Lady Gaga, para a construção do meu discurso videográfico no videoclipe “Electric Guitar”. O principal objetivo é dar continuidade aos discursos audiovisuais de formato subversivo, que consistam em produtos primários complexos os quais contemplem diversos parâmetros de abordagem audiovisual, explorando o sensorial e estabelecendo vínculos com questões de teor histórico, sociológico, cultural etc. O primeiro capítulo apresenta Lady Gaga e seu trabalho artístico que, curiosamente, defende minorias com uma postura favorável da cantora, ainda que ela esteja inserida no star system. O segundo capítulo introduz a temática dos estranhos, explica este conceito a partir de diálogos teóricos e, também, aborda a presença dos estranhos em videoclipes. Já o terceiro capítulo foca no estudo do videoclipe como formato audiovisual, trata da sua relevância na cultura visual e dos seus desdobramentos na mídia. O quarto capítulo volta a tratar dos discursos videográficos de Lady Gaga, porém, com foco na análise de alguns dos seus videoclipes, selecionados de acordo com o tema desta pesquisa. O quinto e último capítulo trata da experiência criativa do videoclipe “Electric Guitar” e do meu objetivo de reforçar as discussões sobre o “estranho” buscando expandir as fronteiras do senso comum a respeito do videoclipe por meio do emprego de novas linguagens, sem promover uma fuga da estética plástica predominante no pop.

Although the house that sits at 1600 Pennsylvania Ave in Washington, DC has had numerous books written about it and its occupants, the President of the United States and the First Lady, there is very little research to be found on the marriage of the two individuals who reside in the White House and, together, sit at the pinnacle of political power. Many argue the Vice-President is second-in-command, but when you think about it, spouses confess and mull over ideas with one another and may never speak to anyone else about those ideas in such an intimate setting. The marriage created by the interpersonal communication between the President and his wife can expose many elements to decision-making, the level of the First Lady's influence over her husband, and the level of respect each has for the sacredness of the traditional institution of marriage. Miss Hayes has researched the marriages and lives of five First Couples (John and Abigail Adams, Franklin and Eleanor Roosevelt, Ronald and Nancy Reagan, Bill and Hillary Clinton, and George W. and Laura Bush) and the marital and family communication styles between the two spouses within the marriage. Those two individuals who occupy the residency of the White House are America's First Couple and the marriage the two create before their arrival in the nation's capital is a crucial element that should play a part in every American's decision before casting his or her vote for the presidency.

Made available in DSpace on 2017-07-21T18:53:11Z (GMT). No. of bitstreams: 1 Resultados e Discussoes.pdf: 625763 bytes, checksum: 0fcdae03788e2bcb5f1e0834574ae458 (MD5) Previous issue date: 2009-05-27 / The use of musts made with apples discarded during classification to in natura trade and the artificial gasification of the fermented, undertake the quality of Brazilian cider. The fermentation process used results in carbon dioxide loss and, consequently, aromatics compounds with large importance to the sensorial attributes of the fermented as well. The product quality could be improved by the use of selected apples cultivars and by the realization of natural gasification process. The objective of this research was the assessment of sparkling obtained by natural gasification process, by the use of selected apples cultivars. In the sparkling elaboration were used apples of Catarina, Granny Smith and Pink Lady cultivars (2007/2008 crop), which were selected because they present physic-chemical characteristics suitable to the process. Six varietal sparklings were elaborated, all by the Champenoise method, and four mixture sparklings, elaborated from a must made by a mixture of the three cultivars, in which the proportions were defined by a statistic planning. The mixture sparklings were elaborated by four different methods: process Chempenoise, artificial gasification, Asti and Charmat, being the two last made in pilot scale. All sparklings were evaluated by physic-chemical and chromatographic analysis, as well as oenology tasting, being classified by the Principal Compounds Analysis (PCA) statistical method. The PCA use, besides the results obtained by the oenology tasting, allowed the varietal sparklings to be classified in groups made by the apple cultivar used in the elaboration and by the glucose source used in the supplementation of the base-fermented. In this way, was evidenced the significant difference between them and, therefore, the need of elaboration of a mixture so that the characteristics of each cultivar would be demonstrated in a unique must. The use of statistic planning allowed the formulation of mixture must with physic-chemical characteristics adapted to the sparkling elaboration, with were evaluated and compared to a commercial apple sparkling made in Switzerland by the Champenoise method. In the oenology tasting, in all the resultant sparklings was identify the lack of freshness and limpidity, indicating the need of the use of apples with higher acidity and more effective filtration processess. The differentiation between the varietal, mixture and commercial sparkling was possible by the use of PCA, on which the chromatographic parameters were the mainly responsible by the differentiation between the varietal and mixture groups, whilst the physic-chemical parameters, specially acidity and turbidity, were the mainly responsible by the isolation of the commercial sparkling in a third group. It wasn’t evidenced significant difference between the elaboration process. The use of natural gasification process and musts elaborated by mixture of selected apple cultivars were promising measures in the obtention of quality natural apple sparklings. / A utilização de mostos formulados por maçãs descartadas na classificação para o comércio in natura e a gaseificação artificial do fermentado comprometem a qualidade das sidras brasileiras. O processo de fermentação utilizado resulta na perda do gás carbônico e, consequentemente, de compostos aromáticos de grande importância aos atributos sensoriais do fermentado. A qualidade do produto poderia ser aprimorada pela utilização de cultivares de maçãs selecionados e pela realização de processos naturais de gaseificação. A avaliação de espumantes obtidos por processos de gaseificação natural, pelo uso de cultivares de maçã selecionados, foi o objetivo deste trabalho. Na elaboração dos espumantes foram utilizadas maçãs dos cultivares Catarina, Granny Smith e Pink Lady das safras de 2007/2008, selecionadas por apresentarem características físico-químicas adequadas ao processamento. Foram elaborados seis espumantes varietais, todos pelo método Champenoise, e quatro espumantes de mistura, elaborados a partir de um mosto formulado pela mistura dos três cultivares, no qual as proporções foram definidas por um planejamento estatístico. Os espumantes de mistura foram elaborados por quatro diferentes métodos, processos Champenoise, gaseificação artificial, Asti e Charmat, sendo os dois últimos realizados em escala piloto. Todos os espumantes foram avaliados por análises físico-químicas, cromatográfica e degustação enológica e classificados pelo método estatístico Análise por Componentes Principais – PCA. A utilização da PCA, em conjunto com os resultados obtidos pela degustação enológica, permitiram classificar os espumantes varietais em grupos formados pelo cultivar de maçã utilizados em sua elaboração e pela fonte de glucose utilizada na suplementação de seus fermentados-base, de modo que foi evidenciada a diferença significativa entre eles e, portanto, a necessidade de elaboração de uma mistura para que as características de cada cultivar fossem demonstradas em um mosto único. A utilização do planejamento estatístico permitiu a formulação de um mosto mistura com características físico-químicas adaptadas a sua aplicação na elaboração dos espumantes, os quais foram avaliados e comparados com um espumante de maçã comercial produzido na Suíça pelo método Champenoise. Na degustação enológica em todos os espumantes foi identificada a falta de frescor e limpidez, indicando a necessidade do uso de maçãs com maior acidez e de processos mais eficazes de filtração. A diferenciação entre os espumantes varietais, de mistura e comercial foi possível pela utilização da PCA, na qual os parâmetros cromatográficos foram os principais responsáveis pela diferenciação entre os grupos varietais e de mistura, ao passo que os parâmetros físico-químicos, em especial a acidez e turbidez, foram os principais responsáveis pelo isolamento do espumante comercial num terceiro grupo. Não foi evidenciada diferença significativa entre os processos de elaboração. A utilização de processos naturais de gaseificação e de mostos formulados por misturas de cultivares de maçã selecionados foram medidas promissoras na obtenção de espumantes naturais de maçã de qualidade.

Bibliography: leaves 466-497.

Bibliography: leaves 466-497. / x, 497 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of English, 2001

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