Not available / text

科学研究費補助金 研究種目:基盤研究(A)(2) 課題番号:09355002 研究代表者:後藤 俊夫 研究期間:1997-1999年度

The digital footprints collected from the prevailing sensing systems provide novel ways to perceive an individual's behaviors. Furthermore, large collections of digital footprints from communities bring novel understandings of human behaviors from the community perspective (community behaviors), such as investigating their characteristics and learning the hidden human intelligence. The perception of human behaviors from the sensing digital footprints enables novel applications for the sensing systems. Bases on the digital footprints collected with accelerometer-embedded mobile phones and GPS equipped taxis, in this dissertation we present our work in recognizing individual behaviors, capturing community behaviors and demonstrating the novel services enabled. With the GPS footprints of a taxi, we summarize the individual anomalous passenger delivery behaviors and improve the recognition efficiency of the existing method iBOAT by introducing an inverted index mechanism. Besides, based on the observations in real life, we propose a method to detect the work-shifting events of an individual taxi. With real-life large-scale GPS traces of thousands of taxis, we investigate the anomalous passenger delivery behaviors and work shifting behaviors from the community perspective and exploit taxi serving strategies. We find that most anomaly behaviors are intentional detours and high detour inclination won't make taxis the top players. And the spatial-temporal distribution of work shifting events in the taxi community reveals their influences. While exploiting taxi serving strategies, we propose a novel method to find the initial intentions in passenger finding. Furthermore, we present a smart taxi system as an example to demonstrate the novel applications that are enabled by the perceived individual and community behaviors

In this thesis, I examine the case of the Canadian-based multinational mining corporation GreyStar resources Ltd. in Colombia. Angosturas is GreyStar’s large-scale gold mining project in the sensitive wet highland of páramo de Santurbán in the northeast of the Andes. Although GreyStar has undertaken explorations in this area since 1994, Colombia’s Ministry of Environment denied the environmental license to the company to start with extractions in 2011. I suggest that the government’s decision must be understood in the context of massive mobilizations against the project in large cities such as Bucaramanga and Bogotá as well as the principle of sustainable development (hereafter SD). The latter forms part of the 1991 Colombian constitution, and thus, through this legal presence, is considered to provide environmental protection in the country. Despite this government’s recent ruling, GreyStar (which renamed itself ‘Eco Oro’ after the 2011 decision) and other mining companies (e.g. Ventana Gold) have continued their quest to gain permission to begin with extractions in Santurbán. I explore why these continued attempts to persuade the government regarding extraction licensing is possible. In doing so, I critically investigate the principle of SD, which is central to the resolution by which the Ministry of the Environment denies the environmental license to Eco Oro (GreyStar). In other words, this thesis asks why SD allows for the classification of large-scale mining as a ‘common-good’ activity, which has negative implications on attempts to designate certain ecosystems (e.g. páramo) as common-goods on the basis that there are to be sustained as such, and therefore, an unequivocal moratorium on large-scale mining in these ecosystems is necessary. What and whose common-good does large-scale mining in sensitive ecosystems represent? I argue that in the scope of SD, commoditized nature is vulnerable to the volatility of markets and corporate profitability. This thesis is a criticism of SD and the limitations it places on hearing certain kinds of languages and discourses that resist the key assumptions of SD. The case study allows for addressing a gap in the existing literature, which is the distinctive situation of no legally considered ethnic minorities (e.g. small farmers, small miners, and the cities). / Thesis (Master, Cultural Studies) -- Queen's University, 2012-09-24 10:28:50.601

No description available.

L’objectif principal de cette recherche est de contribuer au développement de biocapteurs commerciaux utilisant des surfaces de papier comme matrices d’immobilisation, capables de produire un signal colorimétrique perceptible dans les limites sensorielles humaines. Ce type de biocapteur, appelé papier bioactif, pourrait servir par exemple à la détection de substances toxiques ou d’organismes pathogènes. Pour atteindre l’objectif énoncé, ce travail propose l’utilisation de systèmes enzymatiques microencapsulés couchés sur papier. Les enzymes sont des catalyseurs biologiques dotés d’une haute sélectivité, et capables d'accélérer la vitesse de certaines réactions chimiques spécifiques jusqu’à des millions des fois. Les enzymes sont toutefois des substances très sensibles qui perdent facilement leur fonctionnalité, raison pour laquelle il faut les protéger des conditions qui peuvent les endommager. La microencapsulation est une technique qui permet de protéger les enzymes sans les isoler totalement de leur environnement. Elle consiste à emprisonner les enzymes dans une sphère poreuse de taille micrométrique, faite de polymère, qui empêche l’enzyme de s’echapper, mais qui permet la diffusion de substrats à l'intérieur. La microencapsulation utilisée est réalisée à partir d’une émulsion contenant un polymère dissous dans une phase aqueuse avec l’enzyme désirée. Un agent réticulant est ensuite ajouté pour provoquer la formation d'un réseau polymérique à la paroi des gouttelettes d'eau dans l'émulsion. Le polymère ainsi réticulé se solidifie en enfermant l’enzyme à l'intérieur de la capsule. Par la suite, les capsules enzymatiques sont utilisées pour donner au papier les propriétés de biocapteur. Afin d'immobiliser les capsules et l'enzyme sur le papier, une méthode courante dans l’industrie du papier connu sous le nom de couchage à lame est utilisée. Pour ce faire, les microcapsules sont mélangées avec une sauce de couchage qui sera appliquée sur des feuilles de papier. Les paramètres de viscosité i de la sauce et ceux du couchage ont été optimisés afin d'obtenir un couchage uniforme répondant aux normes de l'industrie. Les papiers bioactifs obtenus seront d'abord étudiés pour évaluer si les enzymes sont toujours actives après les traitements appliqués; en effet, tel que mentionné ci-dessus, les enzymes sont des substances très sensibles. Une enzyme très étudiée et qui permet une évaluation facile de son activité, connue sous le nom de laccase, a été utilisée. L'activité enzymatique de la laccase a été évaluée à l’aide des techniques analytiques existantes ou en proposant de nouvelles techniques d’analyse développées dans le laboratoire du groupe Rochefort. Les résultats obtenus démontrent la possibilité d’inclure des systèmes enzymatiques microencapsulés sur papier par couchage à lame, et ce, en utilisant des paramètres à grande échelle, c’est à dire des surfaces de papier de 0.75 x 3 m2 modifiées à des vitesses qui vont jusqu’à 800 m/min. Les biocapteurs ont retenu leur activité malgré un séchage par évaporation de l’eau à l’aide d’une lampe IR de 36 kW. La microencapsulation s’avère une technique efficace pour accroître la stabilité d’entreposage du biocapteur et sa résistance à l’exposition au NaN3, qui est un inhibiteur connu de ce biocapteur. Ce projet de recherche fait partie d'un effort national visant à développer et à mettre sur le marché des papiers bioactifs; il est soutenu par Sentinel, un réseau de recherche du CRSNG. / The main objective of this research is the development of a commercial biosensor immobilized on paper surfaces, able to produce a colorimetric signal detected by human sensorial limits. This kind of biosensor could be used, for example, in the detection of toxic substances or pathogens. To achieve this objective, microencapsulated enzymes fixed on paper are proposed. Enzymes are biological catalysts with a high selectivity that can accelerate the speed of some chemical reactions up to a million times. However, the enzymes are very sensitive substances that lose their functionality easily; it is therefore necessary to protect them from conditions that could damage them. Microencapsulation is a technique that protects the enzymes without totally isolating them from their environment. In fact, microencapsulation entraps the enzymes into a micron size sphere, made of a porous polymer which prevents the enzyme to be released but allows the diffusion of its substrate inside. The microencapsulation process consists in making an emulsion containing a polymer dissolved in an aqueous phase with the desired enzyme, and the wall of the microcapsule is formed by adding a crosslinking agent that forms a polymer network at the interface of the emulsion. The crosslinked polymer solidifies and it encloses the enzyme in the interior of the capsule. Thereafter, this kind of microcapsules are used to give biosensor properties to the paper. Blade coating technique is used in order to immobilize the enzyme capsules on paper because it is the most widely used method in the paper industry. The microcapsules are mixed with a coating suspension and applied on sheets of paper. The viscosity parameters of the suspension and those of the coating are optimized to obtain a uniform coating in order to meet the industry standards. Bioactive paper obtained is first studied to assess whether the enzymes are still active or not after all the treatments because, as described above, enzymes are iii very sensitive substances. An enzyme known as laccase is used, which allows an easy evaluation of its activity. Enzymatic activity was evaluated through existing analytical techniques or new analysis techniques developed in the Rochefort lab. The results demonstrate the possibility to transfer microencapsulated enzyme systems onto paper by blade coating, by using large scale settings, with paper surfaces of 0.75 x 3 m2 modified at speeds ranging up to 800 m/min. Biosensors retained their activity, despite a drying process by evaporation of water using an IR lamp of 36 kW. The microencapsulation technique proposed here is an effective technique to increase the storage stability of the biosensor and its resistance to exposure to NaN3, which is a known inhibitor of this biosensor. This research is part of a national effort in order to develop a commercial device called bioactive paper; it is supported by the NSERC research network Sentinel.

Simultaneous localization and mapping is the technique to construct a 3D map of unknown environment. With the increasing popularity of RGB-depth (RGB-D) sensors such as the Microsoft Kinect, there have been much research on capturing and reconstructing 3D environments using a movable RGB-D sensor. The key process behind these kinds of simultaneous location and mapping (SLAM) systems is the iterative closest point or ICP algorithm, which is an iterative algorithm that can estimate the rigid movement of the camera based on the captured 3D point clouds. While ICP is a well-studied algorithm, it is problematic when it is used in scanning large planar regions such as wall surfaces in a room. The lack of depth variations on planar surfaces makes the global alignment an ill-conditioned problem. In this thesis, we present a novel approach for registering 3D point clouds by combining both color and depth information. Instead of directly searching for point correspondences among 3D data, the proposed method first extracts features from the RGB images, and then back-projects the features to the 3D space to identify more reliable correspondences. These color correspondences form the initial input to the ICP procedure which then proceeds to refine the alignment. Experimental results show that our proposed approach can achieve better accuracy than existing SLAMs in reconstructing indoor environments with large planar surfaces.

No description available.

The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. The thesis is based on four scientific papers that focus on three main criteria; (i) to prepare reagents for large-scale affinity-proteomics, (ii) to present technical advances in barcoding systems for parallel protein detection, and (iii) address challenges in complex sequencing data analysis. In the first part, bio-conjugation of antibodies is assessed at significantly downscaled reagent quantities. This allows for selection of affinity binders without restrictions to accessibility in large amounts and purity from amine-containing buffers or stabilizer materials (Paper I). This is followed by DNA barcoding of antibodies using minimal reagent quantities. The procedure additionally enables efficient purification of barcoded antibodies from free remaining DNA residues to improve sensitivity and accuracy of the subsequent measurements (Paper II). By utilizing a solid-phase approach on magnetic beads, a high-throughput set-up is ready to be facilitated by automation. Subsequently, the applicability of prepared bio-conjugates for parallel protein detection is demonstrated in different types of standard immunoassays (Papers I and II). As the second part, the method immuno-sequencing (I-Seq) is presented for DNAmediated protein detection using barcoded antibodies. I-Seq achieved the detection of clinically relevant proteins in human blood plasma by parallel DNA readout (Paper II). The methodology is further developed to track antibody-antigen interaction events on suspension bead arrays, while being encapsulated in barcoded emulsion droplets (Paper III). The method, denoted compartmentalized immuno-sequencing (cI-Seq), is potent to perform specific detections with paired antibodies and can provide information on details of joint recognition events. Recent progress in technical developments of DNA sequencing has increased the interest in large-scale studies to analyze higher number of samples in parallel. The third part of this thesis focuses on addressing challenges of large-scale sequencing analysis. Decoding of a huge DNA-barcoded data is presented, aiming at phase-defined sequence investigation of canine MHC loci in over 3000 samples (Paper IV). The analysis revealed new single nucleotide variations and a notable number of novel haplotypes for the 2nd exon of DLA DRB1. Taken together, this thesis demonstrates emerging applications of barcoded sequencing in protein and DNA detection. Improvements through the barcoding systems for assay parallelization, de-convolution of antigen-antibody interactions, sequence variant analysis, as well as large-scale data interpretation would aid biomedical studies to achieve a deeper understanding of biological processes. The future perspectives of the developed methodologies may therefore stem for advancing large-scale omics investigations, particularly in the promising field of DNA-mediated proteomics, for highly multiplex studies of numerous samples at a notably improved molecular resolution. / <p>QC 20150203</p>

大型災害頻傳傷亡慘重，若能把握於救災黃金72小時內救出受困民眾，則可望挽回更多寶貴的生命，但災區通訊網路基礎設施常因災害而遭受嚴重損毀，無法正常運作。救災工作在缺乏通訊系統的支援下，因溝通協調的困難而紊亂無章、效率低落。 本研究提出一個可快速恢復特定區域通訊服務的網路，並為其設計通訊的拓撲結構。我們稱該網路為應急蜂巢式行動通訊網路(Contingency Cellular Network)，簡稱CCN網路。CCN網路利用無線電連接災區行動電話網路中斷訊但結構未損的基地台建構而成，具有建置速度快、使用門檻低等多項特點，可支援災區救援的緊急通訊。 本研究中，我們以各毀損基地台通訊範圍內的通訊需求人數與災區毀損程度，作為效益參數，嘗詴在蜂巢式網路的格網架構以及數量有限的緊急通訊設備下，選擇效益較高的位置點配置緊急通訊設備，建立應急蜂巢式行動網路的網路拓撲，此拓撲除追求最大救災效益外，並顧及通訊品質，避免建立負載失衡的連線。我們將問題塑模為一類似圖論中的K-Minimum Cost Spanning Tree (K-Cardinality Tree or KCT)問題，稱為Depth Bounded K-Maximum Profit Spanning Tree問題，並提供數個快速的啟發式演算法，可在緊急時快速地建立應急蜂巢式行動網路拓撲。 / When a catastrophic natural disaster occurs, the efficiency of disaster response operation is critical to life saving. However, communication systems, such as cellular networks, usually crashed due to various causes that make coordination difficult for many disorganized disaster response workers extremely. Unfortunately, rapid deployment of many existing emergency communication systems relies on a good transportation system, which is usually not available in a catastrophic natural disaster. We propose a Contingency Cellular Network (CCN) by connecting disconnected base stations together with wireless links and portable power generators. CCN can support existing mobile phone users with limited capability. Such a system can support a large number of voluntary workers in the early hours of a catastrophic natural disaster, thus saving many lives. Communication traffics, either voice or data, are forwarded hop-by-hop to the external network that remains operational. The efficiency and effeteness of CCN is obviously depends on the topology of such a forwarding network. This thesis addresses the design of forwarding topology aiming to maximize its efficiency. We take the degree of emergency degree of the damage, population of each stricken as the priority measure as well as the amount of emergency recovery resources as the constraint to determine the topology. We model the CCN topology design problem into a Depth Bounded K-Maximum Spanning Tree Problem. The problem is proven NP-hard and we designed an efficient heuristic algorithm (DBTB) to solve it. We also model CCN topology design problem into a Hop Concerned K-Maximum Spanning iii Tree Program and designed a HCTB algorithm to solve it. The simulation results show that DBTB algorithm can control tree depth effectively but HCTB can gain more profit.