<p>Microcontact printing using elastomeric stamps is a technique used in finding new and efficient ways to produce biodetection chips. Microcontact printed, with poly(dimetylslioxane) (PDMS) stamps, patterns of antibodies have been evaluated using fluorescence microscopy, imaging ellipsometry and atomic force microscopy. Fluorescent conjugated polyelectrolytes form non-covalent molecular complexes with Immunoglobulin-γ type antibodies, antigen binding to the tagged antibody result in spectroscopic shifts. Four different conjugated polyelectrolytes (POWT, POMT, PTT, PTAA) in complex with human serum albumin antibodies (aHSA) have been tested with fluorescence spectroscopy. Complexes of POWT and aHSA gave rise to thelargest wavelength shift when exposed to human serum albumin.</p><p>Several types of commercially available fluorescent antibodies and antigens were used to test the specificity of microcontact printed antibodies to different antigen solutions. Using fluorescence microscopy it could not be shown that printed antibody patterns promote specific adsorption of corresponding antigen. It is proposed however that changed surface characteristics of the substrate due to PDMS residues transferred during printing is the main driving force behind antigen adsorption.</p><p>POMT - poly (3-[(s)-5-amino-5-methoxylcarboxyl-3-oxapentyl]-2,5-thiophenylenehydrochloride)</p><p>POWT - poly (3-(s)-5-amino-5-carboxyl-3-oxapentyl]-2,5-thiophenylenehydrochloride)</p><p>PTAA - polytiophene acetic acid</p><p>PTT - poly (3-[2,5,8-trioxanonyl] thiophene)</p>

Microcontact printing using elastomeric stamps is a technique used in finding new and efficient ways to produce biodetection chips. Microcontact printed, with poly(dimetylslioxane) (PDMS) stamps, patterns of antibodies have been evaluated using fluorescence microscopy, imaging ellipsometry and atomic force microscopy. Fluorescent conjugated polyelectrolytes form non-covalent molecular complexes with Immunoglobulin-γ type antibodies, antigen binding to the tagged antibody result in spectroscopic shifts. Four different conjugated polyelectrolytes (POWT, POMT, PTT, PTAA) in complex with human serum albumin antibodies (aHSA) have been tested with fluorescence spectroscopy. Complexes of POWT and aHSA gave rise to thelargest wavelength shift when exposed to human serum albumin. Several types of commercially available fluorescent antibodies and antigens were used to test the specificity of microcontact printed antibodies to different antigen solutions. Using fluorescence microscopy it could not be shown that printed antibody patterns promote specific adsorption of corresponding antigen. It is proposed however that changed surface characteristics of the substrate due to PDMS residues transferred during printing is the main driving force behind antigen adsorption. POMT - poly (3-[(s)-5-amino-5-methoxylcarboxyl-3-oxapentyl]-2,5-thiophenylenehydrochloride) POWT - poly (3-(s)-5-amino-5-carboxyl-3-oxapentyl]-2,5-thiophenylenehydrochloride) PTAA - polytiophene acetic acid PTT - poly (3-[2,5,8-trioxanonyl] thiophene)

Orientador: Maria de Fátima Morethy Couto / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Artes / Made available in DSpace on 2018-08-26T02:09:23Z (GMT). No. of bitstreams: 1 Gorino_VitorHugo_D.pdf: 11718785 bytes, checksum: e51fc99d7027464b8bea5a8af7ad9e28 (MD5) Previous issue date: 2014 / Resumo: O presente trabalho analisa a produção litográfica de Darel Valença Lins (1924 -) e Lotus Lobo (1948 - ) das décadas de 1970 e 1980. Estes artistas desempenharam papeis de destaque na produção e no fomento da litografia artística no Brasil a partir de contextos e referenciais distintos, no Rio de Janeiro e em Minas Gerais, respectivamente. Suas produções autorais apresentam novas direções ao passo que incorporam imagens apropriadas em suas gravuras e, ao mesmo tempo trabalham com novas soluções processuais para as impressões, de forma que suas obras enquadrem-se dentro do grupo de artistas que investigam a gravura como meio expressivo ambivalente, caracterizado pela reprodução e pela multiplicação da imagem. Suas litografias se apresentam de tal forma, que reajustam e recompõem as imagens apropriadas recodificando-as, gerando uma nova construção na qual o registro original não mais as representa, mas também não se apaga por completo, semelhantemente ao que fazem os processos cognitivos de memória / Abstract: This work analyses the artwork of Darel Valença Lins (1924 -) and Lotus Lobo (1948- ) through the 1970's and 1980's. These artists played top roles in the artistic application and teaching of the lithographic printing process in Brazil, specifically in Rio de Janeiro and Minas Gerais respectively. Their personal work show new artistic investigation, placing them among the group of artists that investigate their medium technically and conceptually / Doutorado / Artes Visuais / Doutor em Artes Visuais

This master's thesis deals with the study of electric properties of graphene layers covered by Ga atoms in UHV conditions. The substrates were prepared by using laser litography and the graphene layer was prepared by using chemical vapor deposition (CVD). Dependence of Dirac point location on gallium atoms deposition time and influence of electrical properties of graphene on hydrogen atoms deposition time were studied. Experimental results and their evaluation are discussed.

Graphic Art as Public Education – a Study of the Spreading of Litographic Prints in Swedish Homes 1947-1957  This thesis aims to analyze how graphics as an art form became a tool to democratize art during the period of 1947–1957 in Sweden. Graphic art refers to the collective term that includes multiplied art prints: lithography, etching, woodcut, and serigraphy. I am using Pierre Bourdieu's sociological theories and concepts as a theoretical framework. The first part highlights the various agents whose ambition was to realize the goal that art would be a matter for everyone in society. These agents consisted of organizations and political representatives. The fact that the state guarded good taste and art presented a potential for change in the existing art field and an opportunity for new agents to exert influence. Next, I examine the role of graphics as an art form, in particular lithography, which had its’ big revival in the 1940s. The number of lithographs that were published via Konstfrämjandet and spread across the country testifies to the power of the political goal that art should concern everyone. The process included education via public organizations of adult and childrens education, creating art societies within the workplace and in general contexts, as well as expanding the trading of art via local agents throughout the country. In the last part, I report on my research regarding how communication relating to graphic arts was carried out. Three cultural inquiries (1947, 1953, 1956), intended to form the basis of a modern cultural policy, emphasized the importance of cultural and artistic education of the people. The investigations called for education and the presence of art in homes and workplaces as well as in schools. The communication about the role of graphic arts in the organization’s publications follows the degree of relevance conveyed in the investigations. The summary I present also shows the increase of organization members, exhibition visitors and graphic arts sales during the years 1947–1957, all with the support of the government's democratization work.

The main goal of this work is to introduce with the basic technologies of manufacturing MEMS (Micro-electro-mechanical-systems). Further there is mentioned properties and manufacturing of CNT (Carbon nanotubes), used in manufacturing of capacitance pressure sensor.

O objetivo principal deste trabalho foi desenvolver um dispositivo de filme fino com anisotropia de resistividade elétrica. A idéia foi usar um efeito quântico presente em filmes muito finos de materiais condutores ou semicondutores com morfologia anisotrópica na superfície. A morfologia foi um perfil unidirecional quase-senoidal. As resistividades foram determinadas medindo-se as resistências elétricas destes materiais em direções ortogonais, levando-se em conta a geometria da amostra e suas dimensões. O material condutor usado foi Polimetilmetacrilato (PMMA) com ouro implantado na superfície. A profundidade média de implantação foi 2,7 nm. Na fabricação do dispositivo foi utilizada micro e nanolitografia, caracterização por Microscopia Eletrônica de Varredura e Microscopia de Força Atômica e implantação de ouro por MePIIID (Metal Plasma Immersion Ion Implantation and Deposition). / The main purpose of this work was to develop a thin film device with electrical anisotropic resistivity. The idea was to use a quantum effect which is present in very thin films of conductor or semiconductor materials with anisotropic morphology on the surface. The morphology was a sinusoidal-like unidirectional profile. The resistivities were determined measuring the electrical resistances of theses materials in orthogonal directions, taking in account the sample geometry and dimensions. The conductive material used was Polymethylmethacrylate (PMMA) with gold implanted on the surface. The average implanted depth was 2.7 nm. In the device fabrication were used micro and nanolithography, characterization by Scanning Electron Microscopy and Atomic Force Microscopy and gold implantation by MePIIID (Metal Plasma Immersion Ion Implantation and Deposition).

O objetivo principal deste trabalho foi desenvolver um dispositivo de filme fino com anisotropia de resistividade elétrica. A idéia foi usar um efeito quântico presente em filmes muito finos de materiais condutores ou semicondutores com morfologia anisotrópica na superfície. A morfologia foi um perfil unidirecional quase-senoidal. As resistividades foram determinadas medindo-se as resistências elétricas destes materiais em direções ortogonais, levando-se em conta a geometria da amostra e suas dimensões. O material condutor usado foi Polimetilmetacrilato (PMMA) com ouro implantado na superfície. A profundidade média de implantação foi 2,7 nm. Na fabricação do dispositivo foi utilizada micro e nanolitografia, caracterização por Microscopia Eletrônica de Varredura e Microscopia de Força Atômica e implantação de ouro por MePIIID (Metal Plasma Immersion Ion Implantation and Deposition). / The main purpose of this work was to develop a thin film device with electrical anisotropic resistivity. The idea was to use a quantum effect which is present in very thin films of conductor or semiconductor materials with anisotropic morphology on the surface. The morphology was a sinusoidal-like unidirectional profile. The resistivities were determined measuring the electrical resistances of theses materials in orthogonal directions, taking in account the sample geometry and dimensions. The conductive material used was Polymethylmethacrylate (PMMA) with gold implanted on the surface. The average implanted depth was 2.7 nm. In the device fabrication were used micro and nanolithography, characterization by Scanning Electron Microscopy and Atomic Force Microscopy and gold implantation by MePIIID (Metal Plasma Immersion Ion Implantation and Deposition).

The task of master’s thesis was to perform optimalization process for preparing metal etching mask by electron beam litography and subsequent selective wet ething of silicon with crystalographic orientation (100). Further characterization of etched surface and fabricated structures was performed. In particular, attention was given to the morphology demonstrated by scanning electron microscopy and study changes of the optical properties of gold plasmonic antennas due to their undercut.

The Prague based Haase printing and publishing company played an important role in the development of polygraphy, typography, significantly contributed to the Czech national revival and during its existence collaborated with many artists in some cases of world importance. This PhD thesis tries to bring knowledge to the applied graphic art in the Czech lands in general, but also describes the most important historical events of the Haase company, especially these with influence on its art production. The PhD thesis examines the process of graphic work creation and tries to place selected works in the context of work of individual authors and the fine art in the Central European area in general. It is also focused on the nationality issue and the role it played in the selection of artists with whom the Haase company collaborated.