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Os artefatos e mentefatos nos ritos e cerimônias do Danhono : por dentro do octógono sociocultural A'uwẽ/Xavante /Silva, Adailton Alves da. January 2013 (has links)
Orientador: Ubiratan D'Ambrosio / Banca: Eduardo Sebastiani Ferreira / Banca: Bernadete Ap. Caprioglio de Castro Oliveira / Banca: Dulce Maria Pompêo de Camargo / Banca: Roseli Corrêa Alvarenga / Resumo: O presente trabalho trata de uma pesquisa sobre o processo de geração, sistematização e difusão dos saberes e fazeres dos A'uwẽ/Xavante, a partir da celebração de um dos principais rituais do povo, o Danhono. É uma investigação de natureza qualitativa que se insere, sob o ponto de vista teórico/metodológico, nos pressupostos do programa Etnomatemática na vertente "dambrosiana" que, por sua vez, dialoga transdisciplinarmente com diferentes áreas do conhecimento. Nessa vertente, priorizamos os aspectos antropológicos e políticos relacionando-os com os aspectos culturais da matemática e aos fundamentados políticos e pedagógicos freireano. Durante o desenvolvimento da pesquisa buscamos abordar os saberes, fazeres e conviveres A'uwẽ/Xavante a partir dos mitos, ritos e cerimônias celebradas no processo de formação e autoformação (Danhono) dos adolescentes, os Wapté e as Azarudu, pertencentes ao grupo de idade Nozö'u, no período de 2006 a 2010 nas aldeias pertencentes à Terra Indígena Pimentel Barbosa. Nessa perspectiva, o método de caráter etnográfico foi amplamente utilizado, buscando ressaltar os elementos matemáticos relacionados aos mitos, ritos e cerimônias do povo A'uwẽ/Xavante. Para a composição do corpus, permanecemos nas comunidades investigadas durante um período de 12 (doze) meses acompanhando as atividades cerimoniais e cotidianas. Através desta convivência buscamos entender/compreender a forma como os mitos, ritos e cerimônias estão articulados com os saberes matemáticos do povo. Como desdobramento dessa investigação, tem-se a construção de uma visão teórica/metodológica/epistemológica dos saberes e fazeres do povo A'uwẽ/Xavante a partir do seu funcionamento no interior da cultura / Abstract: The present work is a research about the A'uwẽ/Xavante's knowledge and practices generation, systematization and diffusion process, starting from Danhono people's main ritual celebration. That is a qualitative nature investigation, under the theoretical / methodological point of view, inserted in the presuppositions of the Ethnomathematics program, in the "dambrosiana" aspect which dialogues transdisciplinarily with knowledge different areas. In that aspect, we prioritized the political and anthropological aspects relating them to the mathematics cultural aspects and to Freire's political and pedagogical concepts. During the research development we tried to approach the A'uwẽ/Xavante's knowledge, practices and way of living, starting from the myths, rites and ceremonies celebrated in the (Danhono) adolescents' formation and self-formation process - the Wapté and Azarudu which belonged to the Nozö'u age group in the period of 2006-2010 in the villages belonged to Pimentel Barbosa Indigenous Land. In this direction, the ethnographic character method was widely used, attempting to highlight the mathematical elements related to the A'uwẽ/Xavante people's myths, rites and ceremonies. For the corpus composition, we stayed in the researched communities for 12 months, accompanying the ceremonial and daily activities. Through this familiarity, we tried to understand as the myths, rites and ceremonies are articulated with the people's mathematical knowledge. Unfolding that investigation, we have the A'uwẽ/Xavante's knowledge and practices theoretical/ methodological/epistemological vision construction, up from its functioning in the culture interior / Doutor
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Revisão taxonômica das espécies sulamericanas de ermitões do gênero Pagurus Fabricius, 1775 (Anomura: Paguridae): análises morfológicas e moleculares / Taxonomic review of South American species of hermit crabs of the genus Pagurus Fabricius, 1775 (Anomura:Paguridae): morphological and molecular analysisCampillay, Nicole Alice Olguín 16 April 2012 (has links)
O gênero Pagurus é um táxon heterogêneo de ermitões, com ampla distribuição mundial, descrito há mais de duzentos anos. A sistemática do grupo é complexa com uma longa história de rearranjos taxonômicos. A classificação conta com a inclusão de numerosas novas espécies e separação de algumas inicialmente contidas no táxon estabelecendo-se novos gêneros. Devido à extensa distribuição das espécies que compõem o táxon, foi necessário restringir o objetivo deste estudo. Assim foi avaliado o status taxonômico das espécies que ocorrem nas costas Pacífica e Atlântica da América do Sul, por meio da combinação de análises morfológicas e moleculares das espécies, utilizando dois marcadores genéticos (16S rDNA e Histona 3). As análises taxonômicas mostraram uma alta variabilidade morfológica nas 22 espécies examinadas. As espécies se encaixam perfeitamente em quatro dos onze grupos preestabelecidos dentro de Pagurus. Além disso, foram fornecidos os caracteres morfológicos que definem um desses grupos. Adicionalmente foi incluída uma chave para ajudar na identificação de todas as espécies. As análises independentes dos dados moleculares mostraram resultados contrastantes. O gene mitocondrial foi mais variável e portanto mais informativo, proporcionando uma hipótese mais clara das relações internas entre os membros de Pagurus. Assim, as topologias moleculares resultantes, concordaram em vários aspectos com o reportado nos dados morfológicos das espécies. De modo que, as semelhanças morfológicas foram refletidas na formação dos nós internos. Assim, as análises do gene 16S e H3 mostraram-se concordante com a morfologia, refletindo-se na formação de alguns dos grupos previamente propostos. Como ponto importante, ressalta-se a separação das espécies que compõe o grupo provenzanoi como um táxon diferenciado dentro de Pagurus. Ao mesmo tempo, as análises com o gene H3 mostraram a espécie Propagurus gaudichaudii inserida dentro de Pagurus, questionando a validade taxonômica de Propagurus. Como só foi incluída uma das cinco espécies do gênero, é claramente necessário a inclusão de outras espécies contidas neste táxon, bem como alguns outros genes uma revisão das espécies contidas neste táxon, junto com análises de outros genes, a fim de resolver definitivamente o status taxonômico de Propagurus. / The genus Pagurus is a heterogeneous taxon of hermit crabs, with worldwide distribution which was described more than two hundred years ago. Systematics of the group is complex and with a long history of taxonomic rearrangements. Thus current classification accounts for inclusion of many new species and splitting-off of some of the original species in order to establish new genera. Because of the extensive distribution of the species that conform this taxon it was necessary to restrict the aim of this study. Thus I evaluated the taxonomic status of the species found in the Pacific and Atlantic coasts of South America using a combination of both morphologic and molecular analyses (16S rDNA and Histona 3). The taxonomic analysis showed high morphological variation among all the 21 examined species. The analyzed species seemed to perfectly fit four out of the eleven morphologically pre-established groups of Pagurus. Furthermore I provide morphological characters that define one of these groups. Additionally, I included a key to aid in the identification of all the target species. Independent analysis of the molecular data showed contrasting results. The mitochondrial gene was the most variable and thus the most informative, providing a clearer hypothesis of the internal relationships among members of Pagurus. Therefore, both 16S and H3 analyses were in general agreement with the morphology. Thus, the resultant molecularly based topologies reflected some of the groups previously proposed. It is important to point out that all the included species belonging to the provenzanoi group were clustered together separated from other clades within Pagurus. At the same time, the analysis with the gen H3 showed Propagurus gaudichaudii clustered within Pagurus, thus questioning the taxonomic validity of Propagurus. As I only included one of the five species of the genus, it is obviously necessary to include other the species contained in this taxon, as well as some other genes in order to definitely solve the taxonomic status of Propagurus.
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Max Reger's variations and fugue on an original theme Op.73 : issues of musical structure, performance practice and interpretationKeventsidou, Eleni January 2016 (has links)
The thesis investigates Max Reger's Variations and Fugue on an Original Theme Op. 73, demonstrates the refinement of Reger's composition, and underlines his connection to the great performer Karl Straube. His musical language, dynamic markings and technical problems regarding the tempo, registration and acoustics will be examined by an actual performance in Canterbury Cathedral. Several issues such as cathedral acoustics, mechanical or pneumatic actions and choice of tempi have underpinned this study. Within each variation, the characteristics and techniques of Reger's compositions express his exceptional connection with the art of fugue and, of course, the use of variation technique in the rest of his organ works. The long Introduction falls into five clear sections and is, followed by the wistful mood and resignation of the Original Theme, where the great role of the third bar is often quoted in the course of the variations. Due to the all-pervading chromaticism Op. 73 gives the impression of being completely pantonal. Canterbury Cathedral organ's electro-pneumatic action and acoustics are close to the Leipzig Sauer instrument, and it seems well capable of meeting the challenges of control, polyphonic harmony, mystery and chromatic moods of Op. 73. Approaching the interpretation of Reger's highly demanding Variations and Fugue on an Original Theme Op. 73 through formal analysis and the complicated background of early twentieth century performance practice will be the final goal of the live performance.
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“O CATOLICISMO DOS FAVORECIDOS E DESFAVORECIDOS DOS BAIRROS EM ALTO ARAGUAIA, MATO GROSSO”Lima, Adevaldo Rosa de 11 June 2001 (has links)
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Previous issue date: 2001-06-11 / The symbolic expressions of the catholicism of the urban center more developed against
the districts of the peripheries on the devotions concrete are distinct in relation at the
religious sense. In the districts of the perpheries are less secularized and in the social
urban center are more secularized. The religious realities are directed under the values
in the services to demand the more favorite classes than to demand less favorite,
generally poorer and marginal of the collectivized of districts. The catolicism is
considered as a symbolic expression in the maintenance and in the legitimate the
religious powers across the impregnation of service hierarchical and ecclesiastical
conjugated the advantages and the necessities of social classes and such are the service
demand a priori of salesianos (the clergies and the sisters religious) of the Alto
Araguaia, Mato Grosso. The signification of catholicism in Alto Araguaia is established
as an expert organic body reproducing and legitimating hegemonic power of the social
classes into the society (in Alto Araguaia). In conclusion, the agonies and the consoles
are softened in the sustentation of the religious sense in order to prevent the society at
the social disorders. / O catolicismo romanizado salesiano historicamente representaram a hegemonia do
poder da classe dominante em Alto Araguaia, Mato Grosso. O catolicismo romanizado é
orientado sob os valores hierárquico – religioso, daí o atendimento ás demandas
religiosas favorece a classe favorecida através dos status de poder. As ‘gentes’ menos
favorecidas, geralmente sendo as ‘gentes’ pobres dos bairros e marginais da sociedade
não expressa a ‘representação’ religiosa do corpo orgânico religioso (especialistas ou
teólogos), no caso dos salesianos através da conjugação do poder hierárquico e
eclesiástico na defesa dos supremos valores sociais, nesse caso contribuindo na
socialização e pacificação ao se tratar da demanda salesiana ‘representada’ pela
‘Rainha da Paz’. Paz esta outorgada apenas para a classe favorecida e de status não
respondendo tais demandas ás ‘gentes’ pobres, elas não se alimentam de paz mas, de
alimento. Em conclusão, os sofrimentos e os consolos são suavizados na sustentação do
sentido religioso a fim de prevenir a sociedade ás desordens sociais.
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Cultures of forecasting : volatile and vulnerable nature, knowledge, and the future of uncertaintyBobbette, Adam January 2018 (has links)
Adam Bobbette Cultures of Forecasting: Volatile and Vulnerable Nature, Knowledge, and the Future of Uncertainty Summary This dissertation is a cultural history and ethnography of volatile nature forecasting. It looks at the ways that the future of nature is known in highly unpredictable contexts through a broad history of modernist nature forecasting and an ethnography of state scientists, shamans, and a sultans retinue on the active volcano, Mount Merapi, Indonesia. The project aims to understand how practices of forecasting generate futures, mobilize, and organise anticipation, how time is known, and populations governed. It looks at the way that publics emerge through forecasting technologies, and how futures and nature-culture relations are contested. It follows the practices of scientists in volcano and tsunami observatories, in planes tracking tropical storms, and bunkers dug into active volcanoes; at how instruments and technologies such as seismographs, windows, globes, speakers, and electrical tomography, mediate and transform relations with nature, the future, and governance. It considers too, the role of architecture, shamanism, and the state in appropriating and governing uncertainty. By following the fieldwork of geophysicists and volcanologists in observatories and the edge of the caldera of Mount Merapi, as well as spirit possession practices, and the ritual offerings of a sultan, I demonstrate how practices of forecasting are making contested futures lived in the present, and forging infrastructures and tools for their longevity. Forecasting, I demonstrate, is a cultural technique that negotiates the porous borders between the human, nature, and the future.
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Pesquisa de anticorpos anti-virus da febre aftosa em espécies de animais domésticos e silvestres susceptíveis e não vacinadas, no Pantanal de Mato Grosso do Sul /Paes, Rita de Cássia da Silva. January 2001 (has links)
Orientador: Hélio José Montassier / Banca: Aramis Augusto Pinto / Banca: José Antonio Jerez / Resumo: A pesquisa sorológica usando Imunodifusão em Gel de Ágar (IOGA) e ELISA Bloqueio de Fase Líquida (BFL-EUSA) foram aplicadas nesse estudo no período de 1996 a 1998, para detectar ou titular, respectivamente, anticorpos contra o antígeno VIA (Virus Infection Associated) e as estirpes de referência 01 Campos, A24 Cruzeiro e C3 Indaial, em algumas espécies silvestres e domésticas susceptíveis e não vacinadas. Do total de 383 amostras sanguíneas colhidas, 78 amostras eram de bovinos de vida livre; 39 de búfalos indianos; 139 de ovinos; 63 de suínos domésticos; 49 de porcos do mato e 15 de cervídeos. Todas as espécies investigadas, exceto os cervídeos, apresentaram anticorpos contra o Vírus da Febre Aftosa (VFA). O teste de IDGA detectou 47 (12,3%) amostras positivas ao antígeno VIA, principalmente em bovinos baguás e búfalos. O BFL-ELISA identificou 175 (45,7%) soros com presença de anticorpos a uma das três estirpes virais; Foi encontrado um maior número de amostras positivas contra a estirpe C3 Indaial bem como maiores títulos, em bovinos baguás. Os bubalinos e ovinos apresentaram uma maior reatividade contra as estirpes A24 Cruzeiro e 01 Campos, respectivamente, demonstrando maior número de soros reagentes. No entanto, ambas as espécies revelaram maiores títulos para a estirpe C3 Indaial. Nos suínos domésticos e silvestres, predominou a reatividade a estirpe A24 Cruzeiro. Em bovinos, a maior ocorrência de soros positivos ao teste de IOGA foi encontrada em animais entre 12 a 36 meses de idade, e na prova BFL-ELISA, em animais acima de 36 meses. Nas demais espécies, ambos os testes laboratoriais utilizados demonstraram um maior número de soros reativos em indivíduos adultos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: A serologic survey, using Agar Gel Immunodifusion (AGID) and liquid Phase Blocking-ELISA (LPB-ELISA) were assessed in this study, in the period from to 1996 to 1998, to detect or titer, respectively, antibodies to Virus Infection Associated Antigen (VIAA) and the foot-and-mouth disease virus (FMDV) 01 Campos, A24 Cruzeiro e C3 Indaial reference strains, in non-vaccinated domestic and wild susceptible animal species. A total of 383 blood serum samples were collected from free-ranging cattle (78 samples), water buffalo (39 samples), sheep (139 samples), domestic (63 samples) and wild pigs (49 samples) and deers (15 samples), living in the Pantanal (Nhecolândia region) of Mato Grosso do Sul State, Brazil. All the investigated species, excepting deer, showed positive animais to FMDV antigens. The AGID detected a total of 47 (12,3%) positive animais for VIA antigen, mostly of them were free-living cattle and buffalo. The LPD-EUSA identified 175 (45,7%) serum samples as reactive to FMDV antigens, specially among cattle, buffalo and sheep. The predominant reactivity to FMDV antigens, characterized by the higher number of positives and the higher antibodies titers, were detected to C3 Indaial strain, particularly among the free-Iiving cattle. Buffaloes and pigs showed, in turn, a prominent reactivity to A24 Cruzeiro strain, while sheep reacted more to 01 Campos strain, though buffalo and sheep serum samples had higher antibody titers to C3 Indaial strain. The highest frequency of positive animais to VIA antigen were found in free-ranging cattle, whose age ranged from 12 to 36 months, contrasting to the results of LPB-EUSA that detected the highest rate of positives in the older bovines (~36 months). The older animais from other species demonstrated, in both serological tests, the highest frequency of positivity... (Complete abstract, access undermentioned eletronic address) / Mestre
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Os múltiplos rurais de Cáceres-MT : em meio à aparente homogeneização, um diverso rural transparece /Oliveira, Ana Leticia de. January 2018 (has links)
Orientador: Darlene Aparecida de Oliveira Ferreira / Resumo: Apesar de, inicialmente, o espaço rural de Cáceres - MT aparentar relativa homogeneização na forma da grande propriedade pecuária, um reconhecimento mais próximo demonstra uma realidade bastante diferente. A partir desse entendimento, defende-se aqui a tese de que o espaço rural de Cáceres é diversificado, dinâmico e múltiplo. E em busca dessa comprovação, definiu-se como objetivo geral deste trabalho, reconstruir o processo de organização do espaço agrário de Cáceres, procurando demonstrar sua estrutura atual que é baseada na presença predominante da pecuária e na emergência de novas atividades, ou seja, o caráter diversificado de seu espaço rural. Para a realização da pesquisa utilizou-se, como metodologia central, o método misto de John Creswell no qual se utiliza como método principal o qualitativo, dentro do qual se aninha o quantitativo, sustentado por pesquisas bibliográficas, entrevistas, questionários, trabalho de campo e profundas reflexões. Com o desenvolvimento da pesquisa reconheceu-se a presença marcante da pecuária e da intensa concentração de terras. No entanto, observa-se, também, um rural diversificado, no qual são encontradas realidades bastante distintas que englobam: desde estabelecimentos rurais menores que 10 hectares até outros com mais de 1000 hectares; pecuária extensiva em contraste com a policultura agrícola; trabalho de mão de obra familiar contrapondo-se ao assalariado; atividades agropecuárias e atividades não-agrícolas como o turismo e a agroin... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Although the rural area of Cáceres - MT initially appears relative homogenization in the form of the large livestock farm, a closer recognition shows a quite different reality. From this understanding, it is defended here the thesis that the rural area of Cáceres is diversified, dynamic and multiple. In search of this evidence, it was defined as the main goal of this composition, to rebuild the process of organization of the agricultural area of Cáceres, trying to demonstrate its current structure based on the predominant presence of extensive livestock and on the emergence of new activities, that is, the diversified character of its rural area. In order to carry out the research, the mixed method of John Creswell was used as central methodology, in which the qualitative method is used as the main one, and the quantitative method is inside it, supported by bibliographical research, interviews, questionnaires, fieldwork and deep reflections. With the development of the research it was recognized the remarkable presence of livestock and the intense concentration of land. However, a diversified rural area is also observed, in which, very distinct realities are found, ranging from rural establishments smaller than 10 hectares to others with more than 1000 hectares; the extensive livestock, predominant in some areas, in counterpoint agricultural polyculture in others; the family wage labor and the presence of livestock farming and non-agricultural activities such as tourism and ag... (Complete abstract click electronic access below) / Resumen: Aunque, inicialmente, el espacio rural de Cáceres - MT aparente relativa homogeneidad, en forma de gran propiedad ganadera, un reconocimiento más cercano demuestra una realidad muy distinta. Partiendo de ese entendimiento, se defiende aquí la tesis de que el espacio rural de Cáceres es diverso, dinámico y múltiple. Y en busca de esa comprobación, se definió como objetivo general de este trabajo, reconstruir el proceso de organización del espacio agrario de Cáceres, buscando demonstrar su estructura actual, basada en la presencia predominante de la ganadería y en el surgimiento de nuevas actividades, o sea, el carácter diversificado de su espacio rural. Para la realización de la investigación se utilizó, como metodología central, el método mixto de John Creswell en el que se utiliza como método principal el cualitativo, dentro del cual se anida el cuantitativo, sostenido por investigaciones bibliográficas, entrevistas, cuestionarios, trabajo de campo y profundas reflexiones. Con el desarrollo de la investigación se reconoció la presencia notable de la ganadería y de la intensa concentración de tierras. Sin embargo, se observa, también, un ambiente rural diverso, en el cual se encuentran realidades muy distintas que engloban: desde establecimientos rurales menores de 10 hectáreas hasta establecimientos con más de 1000 hectáreas; ganadería extensiva en contraste con el policultivo agrícola; trabajo de mano de obra familiar contraponiéndose al asalariado; actividades agropecuaria... (Resumen completo clicar acceso eletrônico abajo) / Doutor
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Values of higher popular music education : perspectives from the UKParkinson, Tom January 2014 (has links)
In the 23 years since the first undergraduate popular music degree programme opened in the United Kingdom, the academic discipline of popular music has burgeoned to encompass over 160 programmes delivered across the higher education sector, by private institutions, Royal-chartered conservatoires, post-92 universities and Russell Group universities. This doctoral research project seeks to understand the values underpinning and informing educational practice in this growing academic discipline. It proceeds from an understanding of higher education and popular music as two highly complex domains in their own right, and from the proposition that values inhering at their nexus- Higher Popular Music Education- derive from and are borne by multiple human, institutional and disciplinary sources, and bear the trace of socio-cultural, economic and historical contexts related to each domain. It takes an inductive approach to a multiple-case study of four popular music degree programmes at different higher education institutions across the United Kingdom. Acknowledging from the outset the impossibility of identifying a conclusive ‘roster’ of itemisable values, this study draws on a combination of institutional literature, semi-structured interview and field observation data to explore the interplay of musical, educational and other values within the educational message systems of pedagogy, curriculum, institution, assessment, lifestyle and market. Analysis of the data suggested that seemingly unrelated values such as, for example, those relating to musical aesthetics and social justice, could in fact be oppositional in practice, resulting in surprising tensions and impacting on such areas as curricula and student lifestyles. Moreover, values enshrined in policy, or perceived by interviewees to be dominant within the higher education sector, appeared often to be at odds with individuals’ personal opinions regarding the value of knowledge and education, or with what they saw to be the core values of popular music as an art form. This interdisciplinary study sits across the research fields of music education, the sociology of higher education and popular music studies, and makes original contributions to knowledge in each of these fields.
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Caracterização molecular do vírus Melao cepas BE AR 633512 e BE AR 8033 (Bunyaviridae, Orthobunyavirus) e infecção experimental em hamsters dourados (Mesocricetus auratus)CARVALHO, Valéria Lima 25 May 2007 (has links)
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Previous issue date: 2007 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / As cepas do Virus Melao (VMEL), BE AR 8033 e BE AR 633512 foram isoladas de mosquitos Ochlerotatus (Ochlerotatus) scapularis, em Belém- PA (1955) e Alta Floresta do Oeste- RO (2000), respectivamente. Este trabalho teve como objetivo caracterizar molecularmente as cepas BE AR 633512 e BE AR 8033 e realizar estudos histopatológicos, bioquímicos e imunológicos comparativos em hamsters dourados (Mesocricetus auratus). Hamsters mostraram suscetibilidade às cepas do VMEL. A viremia em hamsters para BE AR 633512 ocorreu do 3º ao 6º dias pós-infecção (dpi.), e para a cepa BE AR 8033 ocorreu no 2º dpi. Anticorpos neutralizantes para ambas as cepas foram detectados a partir de 5 dpi., e se mantiveram até 30 dpi. As cepas testadas alteraram os marcadores bioquímicos AST, ALT e uréia, enquanto que a creatinina só apresentou alteração estatisticamente significante nos animais infectados com a cepa viral BE AR 633512, em comparação aos animais controles não infectados. Alterações histopatológicas foram observadas no SNC, fígado, rim e baço dos hamsters infectados pelas cepas do VMEL, sendo a infecção nesses órgãos confirmada por imunohistoquímica. A cepa BE AR 633512 foi mais virulenta e patogênica para hamsters que a cepa BE AR 8033. A análise genética dos genes N, Gn e Gc revelou que para os genes N e Gn, a cepa BE AR 8033 e do protótipo VMEL (TRVL 9375) são mais geneticamente relacionados. Para o gene Gc, a cepa BE AR 8033 é mais relacionada com a cepa BE AR 633512, sendo que esta última cepa apresentou maior variabilidade genética, principalmente no gene Gn com várias substituições de aminoácidos, mas as mutações no gene Gc provavelmente foram responsáveis pelo aumento da virulência e patogenicidade em hamsters. / The Melao Virus (MELV) strains, BE AR 8033 e BE AR 633512 were isolated from lots of Ochlerotatus (Ochlerotatus) scapularis mosquitoes, in Belém- PA (1955) and Alta Floresta do Oeste- RO (2000), Brazil, respectively. The aim of this study was to carry out molecular characterization of the MELV strains BE AR 633512 and BE AR 8033 and to describe the histopathologic, biochemistry and immunological changes in golden hamsters (Mesocricetus auratus). Hamsters showed susceptibility to these MELV strains. The viremia produced in hamsters by BE AR 633512 strain occurred between 3 and 6 days post-infection (dpi.), and for the BE AR 8033 occurred only in the 2nd dpi. Neutralizing antibodies for both strains were initially detected on the 5th dpi. and remained up to the 30 dpi. The biochemistry markers AST, ALT and blood urea nitrogen showed values statistically significants among inoculated animals with both VMEL strains, while creatinine value it was only altered by BE AR 633512 strain. Histopathologic changes were observed in the central nervous system, liver, kidney, and spleen of the hamsters, and infection was confirmed by immunohistochemical assay in all them. Strain BE AR 633512 caused more intense tissue damage showing increases virulence and pathogenicity than BE AR 8033 strain. The genetic analysis of the N, Gn and Gc genes revealed that for N and Gn genes, the BE AR 8033 and prototype strain (TRVL 9375) are genetically more closed related, and for the Gc gene, the BE AR 8033 and BE AR 633512 strains are more related. BE AR 633512 strain showed increased genetic variability, mainly in the Gn gene, were several aminoacid changes were observed, but the Gc mutations should be responsible for the increased virulence for hamsters.
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Ilegalismos e jogos de poder : (1840-1880)Machado Filho, Oswaldo 31 October 2018 (has links)
Orientador: Paulo Celso Miceli / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas / Made available in DSpace on 2018-10-31T18:32:54Z (GMT). No. of bitstreams: 1
MachadoFilho_Oswaldo_D.pdf: 1406699 bytes, checksum: 9973e93efaa0c343c2b063ef0d4dc40b (MD5)
Previous issue date: 2003 / Doutorado
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