In this thesis a robust Python based software for controlling a Chlorophyll-a Pulse-Amplitude-Modulated (PAM) fluorescence microscope and analysing subsequent data has been developed and validated. The automation software, called PACMan (PAM Automation Control Manager) was made for the purpose of increasing the amount of single cell data generated per experiment. PACMan includes an autofocus algorithm and the ability to vary experimental parameters during experiments. The analysis software, called PAMalysis, processes and facilitates interpretation of PAM experimental data, printing both text files and creating graphical output. PACMan was used on two different phytoplankton species of the Symbiodiniacae family to characterize them under thermal stressors while immobilized on a microfluidic device. The heterogeneity of the phytoplanktons response to increasing thermal stress was evaluated and the best performers under heat stress have been removed using Laser Capture Microdissection for downstream cultivation. PACMan was also used to compare the response of 4 Symbiodiniacae species to increasing relaxation time between light pulses and to image the heterogeneity of response of the common eukaryotic model organism C. reinhartii to a chemical gradient of the common herbicide DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea).

Biomass is a valuable and limited resource that should be used efficiently. The potential of replacing fossil-based products with bio-based ones produced in biobased industrial systems is huge. One important aim of increasing the share of biobased products is to improve the sustainability of systems for production and consumption. Therefore, it is important to evaluate what solutions are available to improve the sustainability performance of bio-based industrial systems, and if they also bring negative impacts. The thesis focuses on assessing the role of biogas solutions in developing sustainable bio-based systems. Such assessments are often quite narrow in their scope and focus on quantitative environmental or economic aspects. This thesis aims at also including feasibility related aspects involving the contextual conditions that are assessed more qualitatively. Biogas solutions are identified as a versatile approach to treat organic materials which are generated in large volumes in bio-based industrial systems. The results show that biogas solutions in bio-based industrial systems (i) improve circular flows of energy and nutrients, (ii) are especially viable alternatives when the quality of the by-product streams become poorer, and (iii) may improve the profitability of the bio-based industrial system. To perform better assessments of these systems, it seems valuable to broaden the set of indicators assessed and include feasibility-related indicators, preferably through the involvement of relevant stakeholders as they contribute with different perspectives and can identify aspects that influence the sustainability in different areas. Future studies could benefit from applying those broader assessments on more cases to build on a more generalisable knowledge base.

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Weathering of silicate minerals has long been a known source of natural CO2 sequestration, that could be increased in the presence of microorganisms. Bio-enhanced weathering of silicate minerals could increase the sequestration of CO2 from the atmosphere.   The aim of this project was to evaluate the potential for a new Neutral emission technology (NET), using four different organisms, Aspergillus Niger, Knufia Petricola, Bacillus Subtilis and Cupriavidus Metallidurans and their potential to increase olivine weathering (dunite). Straw, manure and digestate was used as carbon sources. In total 9 biotic - and 9 abioitc reactors were made, containing a mixture of dunite and one of the three carbon sources. In total 250 mL of water was added to each reactor per week, for 6 weeks, and collected at the end of the week for analysis. Geochemical analyses of the leachate were performed, including pH, conductivity, alkalinity, total organic carbon (TOC), total inorganic carbon (TIC), cations, anions and three organic acids: citrate, acetate, and oxalate. Scanning emission microscope (SEM) was used to monitor potential differences pre- and post-treatment.   Straw reactors produced the most growth, both on the carbon source and the dunite grains. Likely due to the increased labile organic carbon concentrations. The total inorganic carbon and alkalinity demonstrated that inoculation of the reactors promoted weathering for all carbon sources, most significantly for the straw reactors. This observation was evidenced by etch pits in the SEM images and higher TIC, alkalinity, and magnesium values. Microbially enhanced silicate weathering has demonstrated it could be used for the development of NETs for the sequestration of atmospheric carbon. / BAM!

På grund av det stundande ökade elektricitetsbehovet vill Växjö Energi AB utreda möjligheter för utökad kapacitet för elektricitetsproduktion i samband med att ett av deras befintliga kraftvärmeblock börjar nå sin livslängd. En av de tekniker som Växjö Energi, genom detta examensarbete, granskar är Biomass fired Top Cycle. Denna teknik står företaget Phoenix BioPower AB för, där tekniken är specifikt inriktad på att kunna få ut ett högre elutbyte än vad befintliga kraftvärmeverk kan idag. Genom två olika scenarion, där ett Biomass fired Top Cycle block ingår, jämför arbetet bränsleförbrukning, värme- och elproduktion emot VEAB:s befintliga anläggning under ett år. I Scenario 1 var BTC-blocket i drift självt under sommarperioden, medan det i scenario 2 var avstängt och SV3 istället var i drift. Resultatet för de två scenariona visade på en stor ökning för elektricitetsproduktionen, men också en ökning för bränsleförbrukningen. Utifrån elektricitetsproduktionen och bränsleförbrukningen analyserades intäkter och kostnader för ett exempel år 2022. Arbetet konstaterar att välja mellan hög elektricitetsproduktion alternativt hög bränsleförbrukning är en komplex fråga, där både faktorer så som tillgänglighet på biomassa och världens energiläge läggs till.

Since the 1900’s it has been known that microorganisms are capable of generating electrical power through extracellular electron transfer by converting the energy found organic compounds (Potter, 1911). Microbial fuel cells (MFCs) has garnered more attention recently, and have shown promise in several applications, including wastewater treatment (Yakar et al., 2018), bioremediation (Rosenbaum & Franks, 2014), biosensors (ElMekawy et al., 2018) desalination (Zhang et al., 2018) and as an alternative renewable energy source in remote areas (Castro et al., 2014). In MFCs catalytic reactions of microorganisms oxidize an electron donor through extracellular electron transfer to the anode, under anaerobic conditions, with the cathode exposed to an electron acceptor, facilitating an electrical current (Zhuwei, Haoran & Tingyue, 2007; Lovley, 2006). For energy production in remote areas a low cost and easily accessible feed stock is required for the MFCs. Sweet sorghum is a drought tolerant feedstock with high biomass and sugar yields, good water-use efficiency, established production systems and the potential for genetic improvements. Because of these advantages sweet sorghum stalks were proposed as an attractive feedstock (Rooney et al., 2010; Matsakas & Christakopoulos, 2013). Dried sweet sorghum stalks were, therefore, tested as a raw material for power generation in a MFC, with anaerobic sludge from a biogas plant as inoculum (Sjöblom et al., 2017a). Using sorghum stalks the maximum voltage obtained was 546±10 mV, the maximum power and current density of 131±8 mW/m2 and 543±29 mA/m2 respectively and the coulombic efficiency was 2.2±0.5%. The Ohmic resistances were dominant, at an internal resistance of 182±17 Ω, calculated from polarization data. Furthermore, hydrolysis of the dried sorghum stalks did not improve the performance of the MFC but slightly increased the total energy per gram of substrate. During the MFC operation, the sugars were quickly fermented to formate, acetate, butyrate, lactate and propionate with acetate and butyrate being the key acids during electricity generation. Efficient electron transfer between the microorganisms and the electrodes is an essential aspect of bio-electrochemical systems such as microbial fuel cells. In order to design more efficient reactors and to modify microorganisms, for enhanced electricity production, understanding the mechanisms and dynamics of the electron transport chain is important. It has been found that outer membrane C-type cytochromes (OMCs) (including omcS and omcZ discussed in this study) play a key role in the electron transport chain of Geobacter sulfurreducens, a well-known, biofilm forming, electro-active microorganism  (Millo et al., 2011; Lovley, 2008). It was found that Raman microscopy is capable of providing biochemical information, i.e., the redox state of c-type cytochromes (cyt-C) without damaging the microbial biofilm, allowing for in-situ observation. Raman microscopy was used to observe the oxidation state of OMCs in a suspended culture, as well as in a biofilm of an MFC. First, the oxidation state of the OMCs of suspended cultures from three G. sulfurreducens strains (PCA, KN400 and ΔpilA) was analyzed. It was found that the oxidation state can also be used as an indicator of the metabolic state of the cells, and it was confirmed that PilA, a structural pilin protein essential for long range electron transfer, is not required for external electron transfer. Furthermore, we designed a continuous, anaerobic MFC enabling in-situ Raman measurements of G. sulfurreducens biofilms during electricity generation, while poised using a potentiostat, in order to monitor and characterize the biofilm. Two strains were used, a wild strain, PCA, and a mutant, ΔOmcS. The cytochrome redox state, observed through the Raman spectra, could be altered by applying different poise voltages to the electrodes. This change was indirectly proportional to the modulation of current transferred from the cytochromes to the electrode. This change in Raman peak area was reproducible and reversible, indicating that the system could be used, in-situ, to analyze the oxidation state of proteins responsible for the electron transfer process and the kinetics thereof.

Over the past few decades, a growing interest in the thermal conversion of alternative fuels such as biomass and waste-derived fuels has been observed among the energy-producing companies. Apart from meeting the increasing demand for sustainable heat and power production, other advantages such as reducing global warming and ameliorating landfilling issues have been identified. Among the available thermal conversion technologies, combustion in grate-fired furnaces is by far the most common mode of fuel conversion. In recent years, Fluidized-Bed (FB) technologies have grown to become one of the most suitable technologies for combustion and gasification of biomass and waste-derived fuels.In spite of the benefits, however, some difficulties are attributed to the thermal conversion of the alternative fuels. Ash-related issues could be a potential problem, as low-grade fuels may include considerable concentrations of ash-forming elements such as K, Na, S, Ca, Mg, P, Si and Cl. These elements undergo many undesirable chemical and physical transformations during the thermal conversion, and often cause operational problems such as deposition-related issues, slag formation in furnaces, corrosion of the heat transfer surfaces, and bed agglomeration of the fluidized-beds. Ash-related problems in the utility boilers are a major concern that may result in decreased efficiency, unscheduled outages, equipment failures, increased cleaning and high maintenance costs.This thesis investigated the ash behavior and ash-related problems in two different FB conversion systems: a Bubbling Fluidized-Bed (BFB) boiler combusting solid waste, and a Dual Fluidized-Bed (DFB) gasifier using biomass as feedstock. Full-scale measurements, chemical analysis of fuel and ash, as well as thermodynamic equilibrium modeling have been carried out for the BFB boiler (Papers I-IV), to investigate the impact of reduced-bed temperature (RBT) and also co-combustion of animal waste (AW) on the ash transformation behavior and the extent of ash-related issues in the boiler. For the DFB gasifier (Paper V), a thermodynamic equilibrium model was developed to assess the risk of bed agglomeration when forest residues are used as feedstock.The experimental results showed that the RBT and AW co-combustion could decrease or even resolve the ash-related issues in the BFB boiler, resulting in a lower deposit-growth rate in the superheater region, eliminating agglomerates, and a less corrosive deposit (in RBT case). Thermodynamic equilibrium modeling of the BFB boiler gave a better understanding of the ash transformation behavior, and also proved to be a reliable tool for predicting the risk of bed agglomeration and fouling. The modeling of the DFB gasifier indicated a low risk of bed agglomeration using the forest residues as feedstock and olivine as bed material, which was in good agreement following the observations in a full-scale DFB gasifier.

Due to an increasing global population and changes in diets the impact of food consumption on the world’s ecosystems and their services has become more significant. The purpose of this project has been to investigate how the impact on ecosystem services from Swedish food consumption has changed over time and if this development is sustainable or not. A quantitative assessment of six different environmental impact factors has been carried out for each food category. The project clarifies how Swedish food consumption has developed since the 1960s, the origin of the food that has been consumed, which processes that affect the environment as well as the consequences from these impacts. The result showed that Swedish food consumption has increased considering quantity, that the consumption pattern has changed, and that the import of goods has increased since the 1960s. The average Swede eats more animal-based products such as meat, cheese and cream, along with more vegetable-based products such as fruit and berries, and vegetables. Instead, products such as milk and soured based products has decreased, while products made from flour and grains has stayed unchanged. The project shows that animal-based products, especially from ruminant animals, has a significantly higher ecological footprint in comparison with vegetable-based products, concerning most of the categories mentioned above. Considering that the swedes eat more animal-based products today than in the 1960sthefollowing conclusion, that the Swedish impact on ecosystem services has increased since the 1960s even though food production has become more efficient, could be drawn.

Humans are increasingly influencing the climate and the temperature of the Earth by burning fossilfuels, destroying forests, and raising livestock. This adds massive amounts of greenhouse gases(GHG) to those already present in the atmosphere, amplifying the greenhouse effect andcontributing to global warming. The building sector accounts for a significant amount ofgreenhouse gas emissions. Decarbonizing the building industry can result in significant emissionreductions in the future years. Sweden's energy and climate goals have been updated, and some ofthem include reducing GHG emissions in the building sector, increasing energy efficiency, andmaking electricity production 100 percent renewable. In Sweden, energy renovations in singlefamily houses (SFHs) have the potential to reduce GHG emissions and improve energy efficiency,but the rate of energy renovations remains low because of financial, social, and behavioral barriers.This thesis aims to use LCA and LCC methodologies to assess energy renovations on SFH inVäxjö by combining various combinations of energy efficiency measures (EEMs) to reduce energyuse. The energy performance and eight different renovation scenarios using different EEMs havebeen evaluated for the selected single-family building. To evaluate building renovation measures,we developed a method based on life cycle assessment (LCA) and life cycle cost (LCC) thatincorporates building information modeling (BIM). Five different renovation measures werecombined in eight scenarios in this research, including different thicknesses of thermal insulationfor walls and roofs, triple-glazed windows, and doors with different U-values, air-source heatpumps, mechanical ventilation with heat recovery, and solar photovoltaic. The present cost valuesof renovation measures over 50 years for LCC calculation were calculated. The global warmingpotential (GWP) of each renovation measure was estimated over 50 years using One-click LCA.According to the findings of this thesis project, scenarios 1 and 8 had the lowest and highestreductions in primary energy number, respectively. Scenarios 5, 6, 7, and 8 are the most costeffective in comparison to other scenarios. All scenarios resulted in a reduction in GWP impactfrom an LCA perspective in which scenario 7 resulted in the highest reduction in GWP impact.

Access to fresh water is a human right, yet more than 780 million people, especially in rural areas, rely on unimproved sources and the need for finding ways of treating water is crucial. Although the use of natural coagulant protein in drinking water treatment has been discussed for a long time, the method is still not in practice, probably due to availability of material and limited knowledge. In this study, about hundred different crude extracts made from plant materials found in Southern India were screened for coagulation activity. Extracts of three Brassica species (Mustard, Cabbage and Cauliflower) were showing activity comparable to that of Moringa oleifera and were further investigated. Their protein content and profile were compared against each other and with coagulant protein from Moringa. Mustard (large) and Moringa seed proteins were also studied for their effect against clinically isolated bacterial strains. The protein profiles of Brassica extract showed predominant bands around 9kDa and 6.5kDa by SDS-PAGE. The peptide sequence analysis of Mustard large identified the 6.5kDa protein as Moringa coagulant protein (MO2.1) and the 9kDa protein band as seed storage protein napin3. Of thirteen clinical strains analysed, Moringa and Mustard large were proven effective in either aggregation activity or growth kinetic method or both in all thirteen and nine strains respectively. To my knowledge this is the first report on the presence of coagulant protein in Brassica seeds. Owing to the promising results Brassica species could possibly be used as a substitute to Moringa coagulating agent and chemicals in drinking water treatment. / <p>QC 20121214</p>