Solar energy industries are one of the fastest-growing industries in the global energy market. Between 2018 and 2019, installed capacity in Sweden increased by 70%. This is due to a combination of declining PV module and inverter costs, as well as  increased conversion to fossil-free energy production to mitigate greenhouse gas emissions. In fact, solar PVs have a 25-year life span, and soon many deployed PVs would soon reach their end of life (EoL), it is, therefore, important to organize for the EoL of PVs in order to recover precious resources and recycle PV modules in a sustainable manner. Currently, less than 10% of global solar cell waste is recycled, due to the lack of incentives for recycling in most countries. In the European Union, used-up modules are governed by the WEEE (Waste Electrical and Electronic Equipment) Directive, which requires the collection of 85% of solar cell waste, with at least 80% of the waste being prepared for reuse or recycling. Solar cell waste has not amounted to significant volumes in Sweden, due to the lack of no known systems for recycling. Used-up modules are currently collected and managed as electronic waste in one of two approved collection systems in Sweden. The aim of this thesis is to analyze and assess methods of recycling waste solar panels in Sweden and is it economically viable to set up a solar waste recycling center before it reaches the right amount of waste. Moreover, the main focus is on the analysis and comparison of the environmental impacts of various recycling methods for crystalline silicon (c-Si) and cadmium telluride (CdTe) panels. To recycle solar panel waste, the elements of these panels must be assessed from both an economic point of view as well as environmental impacts. Today, the most common PV panels in the global market and also Sweden are c-Si and CdTe types. The results showed except for the pyrolysis method, the environmental impacts of both c-Si and CdTe PV panels from the thermal-based recycling methods, are lower than chemical methods. Furthermore, the extraction of Al, Si, and glass from c-Si and the extraction of glass from CdTe has a less environmental impact than the current techniques used in the recycling of PV panels. Finally, in this study, we revealed which materials can be prioritized for maximum economic and environmental advantages from recycling. In c-Si modules, these are Ag, Al, Si, and glass and in CdTe modules, these are Te, Cu, and glass. Currently, investing in a new solar module recycling center in Sweden is not economically viable. Because the possibility of such an investment requires economic and political incentives. Given that by 2042 the volume of Swedish solar waste will not reach the minimum level of profitability to build a new specialized center for the recycling of solar modules, the best decision is to modify the existing plants in Sweden to recover expensive and vital materials.

I miljöövervakningen av haven runt Sverige har manuell mikroskopi av plankton länge varit den huvudsakliga tekniken för att övervaka växtplanktonbestånden och algblomningar. Nya tekniker utvärderas, men det är inte känt hur resultaten från de nyare teknikerna relaterar till varandra. Två tekniker som utvärderas av SMHI, flödesmikroskopi och DNA-streckkodning, har inte tidigare jämförts i litteraturen. Båda teknikerna har dock jämförts med traditionell mikroskopi. I det här projektet har provserier för DNA-streckkodning och automatiserad mikroskopi med Imaging FlowCytobot (IFCB) samlats in parallellt under en expedition i Egentliga Östersjön, Öresund, Kattegatt och Skagerrak. En bildklassificerare konstruerades med ett konvolutionellt neuronnät, som användes för att klassificera bilderna som tagits med IFCB:n. Resultaten från IFCB:n jämfördes med dem från DNA-streckkodning av 18S rRNA-genen. Jämförelsen visade stark korrelation mellan klassificeringen av bilder och DNA-streckkodning för vissa kiselalger (R>0.8), men teknikernas resultat skilde sig också åt i många fall. Skillnaderna kan studeras för att hitta svagheter i de båda teknikerna och utveckla dem vidare. / In environmental monitoring of the seas around Sweden, manual counting with microscopy is used to monitor the plankton communities and algal blooms. New techniques are currently being evaluated, including imaging flow cytometry and DNA metabarcoding, but it is not known how results from the different techniques relate to one another. Previous work has not compared imaging flow cytometry with metabarcoding, although both methods have been compared to traditional microscopy. In this project, samples for DNA metabarcoding and imaging flow cytometry with the Imaging FlowCytobot (IFCB) have been collected in parallel in the Baltic Proper, Öresund, Kattegat, and Skagerrak. To be able to process the large number of images from cytometry, an image classification algorithm based on convolutional neural networks and transfer learning was developed, which was used to classify the images collected. The results were compared to those obtained with 18S rRNA metabarcoding of the protist community. This new approach of comparing imaging flow cytometry with metabarcoding resulted in a strong (R>0.8) correlation for some diatom taxa, but discrepancies between the technologies were also observed. The discrepancies can be further studied to identify weaknesses in both techniques and refine them further.

Consumption of citrus fruits and citrus juice production creates wastes, which could be valorized by using it for cultivating fungi. Before cultivation, the medium needs to be sterilized though autoclavation. Larger volumes used when autoclaving requires longer heating cycles and therefore runs the risk of degrading the medium to a greater extent. This research examines the effects of the volume lemon waste medium used while sterilizing. The aim is to find the largest volume still providing good growth for the filamentous fungus used, Rhizopus Delemar. Lemon waste was provided by Herrljunga Musteri AB and was pre-treated at 45°C for 2h. The liquid was strained and autoclaved in different volumetric series ranging from 200 – 10 000 mL, that was then used in 200 mL shake flask cultivations.  A scale up in two 3,5 L bubble column reactors was also performed from the 10 000 mL autoclaved medium, after not observing severe impacts on growth. Testing was done by weighing biomass and HPLC analysis of sugars. The yield of the biomass in the shake flasks ranged from 0,11 – 0,14 g/g sugars and the biomass concentration ranged between 2,4 - 3,0 g/L. Overall, the volume of autoclavation seems to not too be of great concern when cultivating R. Delemar on lemon waste medium in the analyzed ranges.

Användandet av deponier är en av de vanligaste metoderna för avfallshantering globalt. Trots insatser som gjordes för att förbjuda hushållsavfall i deponier under millennieskiftet, deponier skapade innan restriktionerna är fortfarande en risk för miljön. Under 2014 öppnade SÖRAB en kontinuerlig biologisk reningsanläggning (KBR-anläggning) på Löt Avfallsanläggning för att hantera lakvatten från en gammal deponi som under en tid fylldes med hushållsavfall. Sedan dess har SÖRAB arbetat med att förbättra KBR-anläggningen. Målet med denna studie är att utforma en driftstrategi för KBR-anläggningen för att förbättra kvävereningen vid låga temperaturer. Ett antal laborativa försök genomfördes, såsom den mikrobiella konsortiets livsduglighet i lakvattnet och tillväxten i både rumstemperatur och vid 4°C, bioaugmentation genom att berika den mikrobiella cellkulturen som redan finns i lakvattnet och hur detta förbättrar kvävereningen i jämförelse med tillsatser av den kommersiella bakterieblandningen ClearBlu Environmental och andra externa kolkällor. Resultaten från dessa laborativa försök påvisade komplett nitrifikation i både rumstemperatur och 4°C i berikat lakvatten från KBR-anläggningens L2A bassäng efter fem dagar. Försöket visade även på syresatt denitrifikation. Dessutom påvisades komplett denitrifikation inom fem dagar, vid rumstemperatur i lakvatten från anläggningens L2B bassäng. Under efterföljande pilotförsök påvisades möjligheten till upplivandet av den biologiska kvävereningen genom berikningen av den mikrobiella cellkulturen i lakvattnet. I ett pilotförsök då lakvatten från L2B bassängen berikades, komplett denitrifikation skedde under en anaerob fas på 16 dagar samt nitrifikation och aerob denitrifikation under ett påföljande 17 dagar lång aerob fas. Ett annat pilotförsök då lakvatten från L2A bassängen berikades påvisade både aerob och anaerob nitrifikation, då ammoniumrening skedde i både den syresatta och syrefria fasen. Tillsatsen av nutrient broth (näringsbuljong) kan påverka KBR-anläggningen, vilket kväver vidare studier. Resultatet från detta projekt tydligt påvisar att kvävereningen i KBR-anläggningen kan förbättras genom att berika den redan närvarande mikrobiella kulturen. / Landfilling has been one of the most popular methods of handling waste globally. Despite the efforts made to stop the disposal of household waste during the turn of the millennia, the landfills formed before these restrictions are still at risk for causing harm to the environment. In 2014, SÖRAB opened a continuous-flow biological treatment (KBR) facility in Löt to treat the leachate produced in one of their older landfills, once filled with household waste. Since then, SÖRAB has been working on improving the treatment facility. The aim of this the study is to find a suitable process to enhance the nitrogen removal at low temperature. Several laboratory scale experiments were performed, such as viability of microbial consortia in the leachate and growth at room temperature and at 4°C, testing bioaugmentation by enriching the microbial cell culture in the leachate and their efficiency in removing nitrogen, compared to the commercial cell culture ClearBlu Environmental and carbon source addition. The results displayed complete nitrification at both room temperature and 4°C in bioaugmented, enriched leachate originating from the L2A basin of the KBR facility, after five days. These trials also suggested the occurrence of aerated denitrification. Complete denitrification within five days was seen at room temperature in bioaugmented, enriched leachate from the L2B basin of the same facility. The ensuing pilot scale trials proved the possibility to revive the biological nitrogen removal by microbial cell culture enrichment. In one pilot in which leachate from the L2B basin was enriched, complete denitrification in the anaerobic phase consisting of 16 days occurred, along with some nitrification and aerated denitrification in the 17 day long aerated phase that followed. Another pilot scale trail in which leachate from the L2A basin was enriched, both aerobic and anaerobic nitrification occurred, as ammonium removal occurred in both the aerated and unaerated phases. The addition of nutrient broth might influence the KBR system which needs further study. The results from this project clearly demonstrate that nitrogen removal in the KBR facility could be enhanced using a culture naturally present in the facility.

The purpose of this thesis is to find out what it takes for entrepreneurs to negotiate strategically in order to ultimately influence systemic change towards sustainability. It focuses on the challenges sustainability entrepreneurs face as they negotiate their twin objectives of social mission and positive cashflow during their collaborative processes. To answer this purpose, I conducted a participatory action research with the entrepreneurs at Hubs Westminster, King’s Cross and Islington in London. The Hub is a global community of people from every profession, background and culture working to tackle the world's most pressing social, cultural and environmental challenges. The Framework for Strategic Sustainable Development (FSSD) was used as a lens for the overall research while the Seven Elements Theory of Negotiation targeted the negotiation process. The thesis demonstrates that some core challenges need to be addressed in the entrepreneur’s preparation for their negotiation; and that entrepreneurs can intentionally favourably position themselves through their strategic actions in their attempt to meet the need to combine economic objectives with concerns about social responsibility and environmentalism. The implication of the thesis lies in better awareness on how to aim at creative outcomes during collaborations. Its originality lies in shedding light in this niche and rarely touched combination of sustainability, entrepreneurship and negotiation.

Innovativa teknologier, produkter och processer krävs på grund av marknadens ökande behov av bioprodukter med låga koldioxidavtryck. Idén att producera flyktiga fettsyror via biologiska processer, främst genom anaerob fermentering med blandkulturer, har återupplivats på grund av den omfattande exploateringen av oljeresurser. Målet för denna studie var att utvärdera kvaliteten och livskraften för produktion av flyktiga fettsyror från enkla substrat. Flyktiga fettsyror-generering undersöktes i ett satsvis syrafermenteringssystem för tre enkla substrat (olja, stärkelse, och gelatin). Vi visade att ett initialt surt pH var fördelaktigt för acidogenes och den totala genereringen av flyktiga fettsyror för alla tre substraten. Den högsta produktionseffektiviteten av flyktiga fettsyror uppnåddes och flyktiga fettsyror genomgick evolutionär utveckling med blandad kulturfermentering som kördes vid ett initialt surt pH på 5,0. 7933,08 mg COD/L smörsyra producerades från stärkelse vid pH 3,87 på dag 15, vilket var den högsta nivån. Smörsyra och ättiksyra var de dominerande produkterna i det initiala sura pH-värdet 5. Alla tre substraten i batch-reaktorer visade högt produktionsutbyte av ättiksyra och smörsyra. Vid lågt initialt pH var propionsyra den vanligaste syratypen i olja. / Innovative technology, products, and processes are required because of the market's rising need for bioproducts with low carbon footprints. The concept of producing volatile fatty acids (VFAs) via biological processes, primarily through anaerobic mixed culture fermentation, has been revived because of the extensive exploitation of oil resources. The goal of this study was to evaluate the quantity and efficiency of VFA production from simple substrates. The generation of VFA was examined using a batch type acid fermentation system for three simple substrates (oil, starch, and gelatin). We showed that an initial acidic pH was advantageous for acidogenesis and the total generation of VFAs for all three substrates. The VFA production efficiency was achieved and underwent evolutionary development with mixed culture fermentation (MCF)  tests running at an initial acidic pH of 5.0. 7933.08 mgCOD/L butyric acid were produced from starch at pH 3.87 at day 15, which was the highest level. Butyric and acetic acids were the predominant products in the initial acidic pH 5. All the three substrates in reactors showed high VFA production yield of acetic acid and butyric acid. At low initial pH, propionic acid was the most prevalent acid type in oil.

The lithium-ion battery (LIB) recycling industry is currently under development to improve the yields for critical metals. However, the organic components of LIBs must also be handled, which may result in harmful chemical emissions as by-products. Of particular concern are highly persistent and mobile per- and polyfluoroalkyl substances (PFAS) that could be released during LIB recycling since some of these compounds have been linked with adverse health effects. In this work, an extensive literature review was conducted to determine the presence of fluorinated materials in state-of-the-art LIBs and the recycling conditions which could lead to the release of problematic PFAS. This information was used to develop a new analytical approach to capture the broadest range of organic and inorganic fluorine species in samples taken in different stages of the recycling process. This new method is based on a sequential extraction procedure using different solvents, followed by combustion ion chromatography (CIC) to quantify the potential emission of fluorine-containing chemicals of different polarities. The results show that organofluorine compounds are formed during recycling, particularly for the cathodes, indicating that PFAS might be present. For other samples, such as the NiMnCo salt product of recycling, only low fluorine levels were detected which implies almost complete removal. Future work should further outline the emission paths of these processes. This study highlights the necessity to further investigate the emissions related to fluorinated materials during LIB recycling and indicates that post-treatments or changes in conditions might be necessary to avoid the formation and emission of PFAS.

Carbon dioxide from biogas production is currently considered to be without value and isbecause of this released into the atmosphere in the biogas upgrading process. The residualgas is a potential carbon source and can create value in the biogas manufacturing process.By finding a suitable value-creating process that utilizes carbon dioxide, it can be possibleto provide both economic and environmental incentives for companies to develop theiroperations. This project explored the possibility to create value from this CO2. Through anevaluation of the technical maturity of CCU technologies, a recommendation could be givenat the end of the project. An analysis of technical barriers, such as pollutants in the gas, aswell as barriers in the form of competence and corporate culture were examined in orderto provide a reasoned recommendation. The project mapped which value-creating systemswould be suitable for biogas producers in a Swedish context. This included established methaneand carbon dioxide upgrading techniques currently in use and suitable CCU techniquesthat can interact with the selected upgrading processes and serve as value creators. Based onthis survey, it was then possible to identify common, critical variables for these systems. Thereafter,a recommendation of an appropriate CCU technology could be given depending onthe CO2 composition produced. One conclusion from the study was that carbon dioxide concentrationsfrom the residual gas was often high (approx. 97-98 %) and did not contain anycorrosive or toxic components, and that this largely depends on how the digestion reactor ishandled in the production process. Thus, questions were raised about what the actual limitationsof the CCU are, as they did not seem to be technical. CCU techniques that proved to beof particular interest were pH regulation of sewage plants, CO2 as a nutrient substrate for thecultivation of microalgae, and manufacturing of dry-ice for refrigerated transports. All of thesetechnologies currently have a sufficiently high degree of technical maturity to be installedalready today. Other CCU techniques, such as "’Power to gas”, require a high CO2 concentrationand were discarded as the literature review did not suggest the economic potential forthem as they require additional CO2 upgrading steps. Instead, CCU techniques were chosenthat could be implemented directly with the existing CO2 quality. Furthermore, it was concludedthat one reason why CCU technologies have not been widely implemented is internalbarriers between distributors and manufacturers (or users) of CCU technologies. Thus, theuse of carbon dioxide from biogas production and implementation of CCU technologies canbe promoted by eliminating barriers in companies, such as a lack of both knowledge andfinancial incentives. / Koldioxid från biogasproduktion betraktas i dagsläget som utan värde och släpps ut i atmosfärenvid uppgradering av biogas. Restgasen är en potentiell kolkälla och kan vara värdeskapandeför biogasprocessen. Genom att finna en lämplig värdeskapande process som utnyttjarkoldioxid går det att ge både ekonomiska och miljömässiga incitament till företag att utvecklasin verksamhet. I detta projekt undersöktes möjligheten att skapa värde av denna CO2.Genom en utvärdering av den tekniska mognadsgraden hos CCU-tekniker kunde en rekommendationges vid projektets slut. En analys av tekniska hinder, såsom föroreningar i gassammansättningen,såväl som hinder i form av kompetens och företagskultur undersöktes för attkunna ge en motiverad rekommendation. I projektet kartlades vilka värdeskapande systemsom skulle passa för biogasproducenter i en svensk kontext. Detta inkluderade etableradeuppgraderingstekniker för metan- och koldioxid som används i dagsläget. I projektet undersöktesäven lämpliga CCU-tekniker som kan samverka med de valda uppgraderingsprocessernaoch och agera värdeskapande. Utifrån denna kartläggning kunde det sedan anges vilkagemensamma, kritiska variabler som finns för dessa system. Därefter kunde en rekommendationav lämplig CCU-teknik ges beroende på den producerade CO2 sammansättningen. Enslutsats i projektet var att koldioxid från restgasen ofta var av hög koncentration (ca. 97-98 %)och ej innehöll några korrosiva eller toxiska komponenter, och att detta till stor del beror påhur rötkammaren är hanterad i produktionsprocessen. Således väcktes frågor kring vilka defaktiska begränsningarna för CCU är, då de inte torde vara tekniska. CCU-tekniker som visadesig vara av särskilt intresse var pH-reglering av avloppsverk, CO2 som näringssubstratför odling av mikroalger, samt tillverkning av kolsyreis för kyltransporter. Samtliga dessatekniker har tillräckligt hög teknisk mognadsgrad för att kunna installeras i dagsläget. AndraCCU-tekniker, såsom ”Power to gas”, kräver en hög CO2-koncentration och avfärdades dålitteraturstudien inte talade för den ekonomiska potentialen i dessa eftersom de kräver ytterligareuppgraderingssteg för CO2. Således valdes istället CCU-tekniker som skulle gå attimplementera direkt med den befintliga CO2 kvalitén. Vidare drogs slutsatsen att en anledningtill att CCU-tekniker inte har blivit vida implementerade till stor del är interna hindermellan distributörer och tillverkare (eller utnyttjare) av CCU-tekniker. Således kan användandetav koldioxid från biogasproduktion och implementering av CCU-tekniker främjasgenom att eliminera hinder hos företag. I projektet yttrade sig detta som bristande ekonomiskaincitament och okunskap. Ett ökat användande av CCU-tekniker kan också uppnås genomatt införa lagar och regler som begränsar användandet av föråldrade tekniker som drivs avfossila bränslen, och som kan ersättas av klimatvänliga CCU-tekniker.

Förlust av biologisk mångfald är den planetära gräns som överskrids mest, och medför bland de största hoten mot biosfärens integritet. Reptiler är funktionella delar i ekosystem, men habitatförlust, förändrad markanvändning och klimatförändringar orsakar globala populationsminskningar. Ekologisk övervakning möjliggör förståelse för arters tillstånd, och utveckling av bevarandeåtgärder. Denna förekomststudie syftar till att undersöka lokal reptilfauna, som inventerades med artificial cover objects (ACO) i två naturområden på Billingen, ett av de västsvenska platåbergen, i Skövde kommun 2021. Data från miljöbeskrivning och inventering samt över väderförhållanden samlades in, och samband mellan observationer och miljöfaktorer testades med statistiskt t-test för positiva skillnader mellan datagrupper. Skogsödla (Zootoca vivipara), kopparödla (Anguis fragilis), snok (Natrix natrix) och huggorm (Vipera berus),alla allmänna reptilarter i regionen, observerades i undersökningsområdet. Nio observationer gjordes under 20 regnfria dagar i juni och juli, med temperaturer över 18°C. Biotopkvaliteterna brynmiljöer och nektarresurser, solexponering och de öppna biotoperna beteshage och elledningsgata, förekom i signifikant högre utsträckning där observationer gjordes, än på platser utan observationer. Markfuktighet, mänsklig närvaro, naturvärden och områdesskydd uppvisade ingen signifikans. Resultaten överensstämmer med andra studier om reptilarternas habitat, och kan ses gälla på lokal nivå trots ett relativt litet dataunderlag. Effektiva reptilbevarande åtgärder i närområdet kan innebära upprätthållande och skapande av ytor med hög solinstrålning och strukturell komplexitet, fortsatt men inte utökat lågintensivt naturbete, samt ökad halt avträdslagsblandning och brynmiljöer i produktionsskog. Kunskapen kring reptilers ekologi, samtövervakningen av populationer, behöver utökas. Det rekommenderas vidare studier om reptiler, deras habitat och bevarande av biologisk mångfald på lokala nivåer. / Biodiversity loss is the most exceeded planetary boundary, and entails some of the largest threats against biosphere integrity. Reptiles are functional parts of ecosystems, but habitat loss and changes in land use and climate are causing global population declines. Ecological monitoring enables understanding of species’ conditions, and development of conservation measures. This occurrence study aims to investigate local reptile fauna, surveyed using artificial cover objects (ACOs), in two areas on Billingen, one of Western Sweden’s table mountains, in Skövde municipality 2021. Environmental, survey and weather data were collected, and relations between observations and environmental factors were tested with statistical t-test for positive differences between data groups. Zootoca vivipara, Anguis fragilis, Natrix natrix and Vipera berus, all regionally common reptile species, were observed in the investigated area. Nine observationswere made during 20 rain-free days in June and July, with temperatures above 18°C. Solarexposure, biotope qualities Forest edges and Nectar resources, and the open biotopes Pasture and Powerline corridor, occurred in significantly higher extent where observations were made, than on sites without observations. Soil moisture, Human presence, Natural values and Environmental protection did not exhibit any significance. The results correspond to other studies on the reptilespecies’ habitat, and may apply on a local scale despite a relatively small data amount. Effective conservation measures for local reptiles can imply maintaining and creating spaces with high solar radiation and structural complexity, continued but not extended low-intensity grazing, and increasing tree species mixture and edge zones in production forests. Knowledge on reptile ecology and population monitoring needs to increase. Further studies on reptiles and biodiversity conservation on local scales are recommended.