The objective of this laboratory research was to investigate the effects of a higher water-cementitious materials ratio on selected properties of concrete mixtures comprising natural pozzolans. The scope of work included testing of six concrete mixtures, including one for each of three natural pozzolans at two water-cementitious materials ratios of 0.44 and 0.48 and one concrete mixture without pozzolan at a water-cementitious materials ratio of 0.44, which was treated as a baseline in this research. The stiffness and strength of each concrete mixture were measured at 1, 3, and 7 days using concrete specimens that were cast immediately after mixing. Additionally, to investigate the effects of delayed casting time, slump was measured at 0, 15, 30, 45, and 60 minutes after mixing, and cylinders were cast at 15, 30, 45, and 60 minutes for stiffness and strength testing at 7 days. Two mixtures comprising natural pozzolan experienced greater slump loss, on average, than the baseline mixture, while all of the other mixtures experienced less slump loss, on average, than the baseline mixture. Overall, the slump losses of mixtures comprising natural pozzolans were 121% and 71% of that of the baseline mixture for water-cementitious materials ratios of 0.44 and 0.48, respectively. Modulus of elasticity values ranged from 1692 ksi to 1794 ksi for mixtures comprising natural pozzolan compared to a value of 1791 ksi for the baseline mixture at 7 days. Compressive strength values ranged from 4087 psi to 4152 psi for mixtures comprising natural pozzolan compared to a value of 4795 psi for the baseline mixture at 7 days. The modulus of elasticity values of mixtures comprising pozzolans were 97% and 94% of that of the baseline mixture for water-cementitious materials ratios of 0.44 and 0.48, respectively, at 7 days. Similarly, the compressive strength values of mixtures comprising pozzolans were 86% and 71% of that of the baseline mixture for water-cementitious materials ratios of 0.44 and 0.48, respectively, at 7 days. Comparisons of the 7-day stiffness and strength results associated with casting delay time for mixtures comprising natural pozzolan with those of the baseline mixture indicate that all mixtures comprising natural pozzolan exhibited lower modulus of elasticity and compressive strength than the baseline mixture. Overall, the modulus of elasticity values of mixtures comprising natural pozzolans were 94% and 84% of that of the baseline mixture for water-cementitious materials ratios of 0.44 and 0.48, respectively, for a casting delay time of an hour. Similarly, the compressive strength values of mixtures comprising natural pozzolans were 85% and 64% of that of the baseline mixture for water-cementitious materials ratios of 0.44 and 0.48, respectively, for a casting delay time of an hour.

Within the vestibular system are otolith organs, both the utricle and saccule. The primary function of these organs is to transduce linear head accelerations and static head tilts into afferent signals that are sent to the central nervous system for the utilization of image fixation, muscle posture control, and the coordination of musculoskeletal movement in dynamic body motion. The utricle of the red ear slider turtle was studied in this dissertation. The turtle's utricle is composed of several layers. The base layer contains a set of neural receptor cells, called hair cells, and supporting cells. The three layers above the base layer compose the utricle's otoconial membrane (OM) and are: 1.) a saccharide gelatinous layer, 2.) a column filament layer, and 3.) a calcite and aragonite otoconial crystal layer. The primary goal of this research was to study the dynamic response of the turtle's OM to a variety of natural inertial stimuli in order to characterize its inherent mechanical properties of natural frequency ("n), damping ("), and shear modulus (G). The medial-lateral (ML) and anterior-posterior (AP) anatomical axes parameters were measured for the utricle. The ML axis median with 95% confidence intervals was found to be "n = 374 (353, 396) Hz, " = 0.50 (0.47, 0.53), and G = 9.42 (8.36, 10.49) Pa. The AP axis median with 95% confidence intervals was found to be "n = 409 (390, 430) Hz, " = 0.53 (0.48, 0.57), and G = 11.31 (10.21, 12.41). Nonlinearites were not found to occur in the OM for the tested inertial stimuli and no significant difference was found between the mechanical properties for the ML and AP axes. Additionally, this research presents the initial steps to form a novel bio-inspired accelerometer based on the morphology of the utricle. The primary transducer element for this possible otolith organ inspired accelerometer design is a droplet interface bilayer (DIB). A DIB is a lipid bilayer that is formed when the interface of two aqueous droplets, that contain free-floating lipids, are joined. The aqueous droplets are suspended in a nonpolar environment (oil) and the oil/water interface forms a lipid monolayer. This research developed and used an experimental test setup to characterize the mechanoelectrical characteristics of a micron-sized DIB. This information, along with examples in the text, could be used to further design the aforementioned accelerometer. / Ph. D.

Collagen type I is an extracellular matrix (ECM) protein that affords tensile strength and biological scaffolding to numerous vertebrate and invertebrate tissues. This strength has been attributed to the triple-helical structure of the collagen type I molecules, their organization into fibrils, and the presence of inter-molecular, covalent, enzymatic crosslinks. There are several different types of these crosslinks; their composition is tissue-specific and dependent upon factors such as age and health. Furthermore, these enzymatic crosslinks tend to form specifically at amino/N- and carboxy/C-terminal crosslinking sites. The mechanical behavior of collagen type I has been investigated, via experiment and theory, at the level of the molecule, microfibril, fibril, and fiber. However, the influence of different enzymatic crosslinks and their location (e.g., N- vs. C-site) on the mechanics of collagen type I has not been investigated in the literature. We employed molecular dynamics to model the mechanical behavior of uncrosslinked and crosslinked ~23-nm-long molecular segments and ~65-nm-long microfibril units of collagen type I. We then used these molecular simulations to construct a model of a single collagen type I fibril by considering the ~65-nm-long microfibril units arranged in series and then in parallel. When a uniaxial deformation was applied along the long axis of the molecular models, N-crosslinks aligned rapidly at lower strains followed by C-crosslinks more gradually at higher strains, leading to a two-stage crosslink recruitment. Then when comparing the influence of different enzymatic crosslinks, significant differences were observed for the high-strain elastic moduli of our microfibril unit models, namely and in increasing order, uncrosslinked, immature crosslinked (HLKNL and deH-HLNL), mature HHL-crosslinked, and mature PYD-crosslinked. At the fibril level, our low- and high-strain elastic moduli were in good agreement with some literature data, but in over-estimation of several other literature reports. Future work will seek to address simplifications and limitations in our modeling approach. A model such as this, accounting for different enzymatic crosslink types, may allow for the prediction of the mechanics of collagen fibrils and collagenous tissues, in representation of healthy and diseased states. / Ph. D.

The movement and control of the vocal folds within the laryngeal cavity enables three crucial physiological functions: 1) allowing respiration by opening, 2) aiding in airway protection by closing, and 3) regulating sound production during phonation. Although treatment options have improved, many of the estimated 7.5 million individuals in the United States who are annually affected by voice-related disorders still face serious challenges related to dysphonia and dysphagia. The need for improved voice-disorder treatments has motivated the work presented in this dissertation which focuses on modeling and manufacturing the vocal folds and aims to answer three main questions: 1) what are the mechanical properties of the vocal folds and how do they change across the full vocal range? 2) how do those properties influence the dynamic behavior of the tissue? and 3) can we manufacture a synthetic vocal fold model that exhibits a desired and controllable dynamic behavior? First, the elastic properties of sixteen porcine vocal folds were evaluated through uniaxial tensile tests on a custom built experimental setup. Stress-strain data was analyzed using an optimization method to yield continuous model parameters which described the linear and nonlinear elastic regions as well as transition points between those regions. Next, the impact of the vocal fold elastic properties on the frequencies of vibration was evaluated through dynamic tests on excised porcine larynges. Sound data was analyzed via a spectrogram and through the use of fast Fourier transforms to study changes in the frequency of vibration while the vocal folds were stretched. Additionally, a mathematical aeroelastic model of phonation was implemented to further evaluate the changing elastic properties on vocal fold dynamics. Next, eight synthetic vocal fold models were created, each with varying mechanical properties and a geometry based on reported anatomical measurements of porcine vocal folds. The synthetic models were then dynamically tested to further study the impact of changes in mechanical properties on the dynamic behavior of the synthetic vocal folds. / Doctor of Philosophy / The movement and control of the vocal folds within the voice-box enables three crucial physiological functions: 1) allowing respiration by opening, 2) aiding in airway protection and swallowing by closing, and 3) regulating sound production during vocalization. Although treatment options have improved, many of the estimated 7.5 million individuals in the United States who are annually affected by voice-related disorders still face serious challenges related to speech production and swallowing which often results in significant detrimental impacts to quality of life. The need for improved treatments is most easily observed in the evaluation of treatment options following a total laryngectomy, which is a procedure where the entire voice-box is removed often due to cancer. Following a laryngectomy, all three of the vital functions of the vocal folds are immediately impacted as patients adjust to breathing through and protecting a redirected airway and are forced to use alternative methods of speech production which often result in monotone or robotic-sounding speech. The need for improved voice-disorder treatments has motivated the work presented in this dissertation which focuses on modeling and manufacturing the vocal folds and aims to answer three main questions: 1) what are the mechanical properties of the vocal folds? 2) how do those properties influence the dynamic behavior of the tissue during sound production? and 3) can we manufacture synthetic vocal folds that produce a desired and controllable dynamic behavior? Sixteen porcine vocal fold samples were mechanical tested to evaluate the elastic properties of the tissue. Next, porcine voice-box samples were experimentally tested in a way that simulated sound production by subjecting the samples to a heated and humidified air flow, similar to the air flow conditions coming out of the lungs. In this way, the relationship between the tissue properties and the frequencies of sound was investigated. Lastly, the synthetic vocal fold samples were evaluated using a similar experimental protocol to further investigate the impact of changing structural properties on the dynamics of the vocal folds during sound production.

Yes / Liquid crystal is a new emerging biomaterial. The physical property of liquid crystal plays a role in supporting the adhesion of cells. Nano and microball indentation techniques were applied to determine the elastic modulus or viscoelasticity of the cholesteryl ester liquid crystals in the culture media. Nano-indentation results (108 ± 19.78 kPa, N = 20) agreed well with the microball indentation (110 ± 19.95 kPa, N = 60) for the liquid crystal samples incubated for 24 hours at 37o C, respectively. However, nanoindentation could not measure the modulus of the liquid crystal (LC) incubated more than 24 hours. This is due to the decreased viscosity of the liquid crystal after immersion in the cell culture media for more than 24 hours. Alternatively, microball indentation was used and the elastic modulus of the LC immersed for 48 hours was found to decrease to 55 ± 9.99 kPa (N = 60). The microball indentation indicated that the LC did not creep after 40 seconds of indentation. However, the elastic modulus of the LC was no longer measurable after 72 hours of incubation due to the lost of elasticity. Microball indentation seemed to be a reliable technique in determining the elastic moduli of the cholesteryl ester liquid crystals. / Science Fund Vot. No. S024 or Project No. 02- 01-13-SF0104 and FRGS Vot. No. 1482 awarded by Malaysia Ministry of Education

Soil stabilization is a widely used technique in the field of geotechnical engineering for a wide range of applications. Traditional stabilizers such as cement and lime, although very efficient, are not environmentally friendly as they leave major carbon footprints, therefore the demand for sustainable stabilization methods is escalating. This research investigates the potential of two different polymers e.g., a biopolymer derived from organic source, and an inorganic commercially manufactured polymer, as viable alternatives for soil stabilization. The current study focuses on exploring the efficacy of polymers stabilized soil in improving the engineering or geotechnical properties such as plasticity, compressibility, shear strength, and stiffness behavior.The research methodology involves using locally available high plastic clay for stabilization using two different types of polymers and performing laboratory experiments to analyze the strength parameters of the stabilized soil. Xanthan Gum (XG) is a biopolymer which is being studied is used in the percentages of 0.5%, 1.0% and 1.5% by dry weight of soil mass to understand the mechanism of biopolymer-soil interactions and to conclude optimum percentage suitable for stabilization in terms of technical and economical value. Similarly, Soiltac (ST) a vinyl copolymer inorganic polymer is used in 1.5% of dry mass of soil (optimum dosage as per previous literature) to compare its effectiveness with that of Xanthan Gum. After the determination of Atterberg limits and Optimum Moisture Content (OMC) and Maximum Dry Density (MDD), the samples were subjected to tests such as Unconfined Compressive Strength (UCS), Ultrasonic Pulse Velocity (UPV), Resilient Modulus (RM) test and Consolidation test. The prepared UCS samples were cured for 0, 7, 14, and 28 days in open air condition before performing test on them. Atterberg limits test on untreated Carbondale Soil were conducted to classify the soil as CH (Clay with high compressibility) type as per USCS (Unified Soil Classification System) classification. While tests on treated sample showed significant increasement in Liquid Limit (LL), slight increment in Plastic Limit (PL), thus quite surge in the Plasticity Index (PI) with increase in XG percentage in the soil. UCS value increased with the increase in percentage addition of XG. Also, UCS results from both untreated and polymer treated samples showed increase in compressive strength with increase in curing period. UCS value increased from 417.75 psi to 490.24 psi, 504.05 psi, and 542.91 psi for 0.5%, 1.0%, and 1.5% XG addition, respectively. This increase in UCS value was 17.35%, 20.66%, and 29.96% for the corresponding XG concentrations. The treated samples had a significant increase in the UCS for all the curing period in comparison to their respectively cured untreated sample. The percentages increase in the UCS for 1.5% XG sample in comparison to untreated sample cured for the same period is 6.45%, 59.57%, and 29.96%, respectively for 7, 14 and 28 days of curing. However, for the zero-day test, the UCS of 1.5% XG stabilized sample was found to be less than the zero-day untreated sample. With the addition of ST polymer, the UCS value increased for all the curing period while comparing with the UCS of untreated soil for the same curing period. The UCS of the ST treated soil increased from 58.56 psi to 467.367 psi when cured for 0 and 28 days which is an increase of 698.1 % i.e. 7 times the strength at 0 day. When UPV (Ultrasound Pulse Velocity) tests were compared with the UCS value for the same sample, the result showed that the higher UPV value corresponded to the higher UCS value. This relationship was supported by the high degree of correlation between the two measurements. The consolidation test showed that the Compression Index (Cc) of XG stabilized soil decreased as the percentage of XG added increased. Cc decreased from 0.2795 for pure Carbondale Soil (CS) to 0.2003 for 1.5% XG addition which is a drop of 28.33%. Likewise, Cc decreased by 3.0% and 19.33% for 0.5% and 1.0% XG doses respectively. The primary aim of this study is to simplify the understanding of the Resilient Modulus (RM) test, which yields vital data for pavement design. The efficacy of inclusion of stabilizer was further substantiated by RM testing which confirmed the enhancement of soil resilient qualities compared to the untreated soil. The RM values exhibited a growing trend, indicating an enhancement in the soil's stiffness and capacity to endure repetitive loads. This attribute is extremely important for applications such as the construction of pavements and foundations that are subjected to dynamic loads. The samples containing 1.0% XG showed significant increases in their RM values. Specifically, the RM values increased by 18.5%, 40%, and 39.5% after being cured for 7, 14, and 28 days, respectively, at a confining pressure of 6 psi. Similarly, the RM for the case of ST ranges from 15227.60 psi for 0 days of curing and 2 psi of confining stress to 45375 psi for 28 days of curing and 6 psi of confining pressure. The performance of ST against XG is higher.

Over the past 50 years, several advancements have been made in the research area of the mechanics of unsaturated soils. These advancements can be categorized into two groups; (i) development (or improvement) of testing techniques (or apparatus) to determine the mechanical properties of unsaturated soils and (ii) development of (numerical, empirical or semi-empirical) models to estimate the variation of mechanical properties of unsaturated soils with respect to suction based on the experimental results. Implementation of the mechanics of unsaturated soils in conventional geotechnical engineering practice, however, has been rather limited. The key reasons for the limited practical applications may be attributed to the lack of simple and reliable methods for (i) measuring soil suction in the field quickly and reliably and (ii) estimating the variation of mechanical properties of unsaturated soils with respect to suction. The main objective of this thesis research is to develop simple and reliable techniques, models or approaches that can be used in geotechnical engineering practice to estimate sol suction and the mechanical properties of unsaturated soils. This research can be categorized into three parts. In the First Part, simple techniques are proposed to estimate the suction values of as-compacted unsaturated fine-grained soils using a pocket penetrometer and a conventional tensiometer. The suction values less than 300 kPa can be estimated using a strong relationship between the compressive strength measured using a pocket penetrometer and matric suction value. The high suction values in the range of 1,200 kPa to 60,000 kPa can be estimated using the unique relationship between the initial tangent of conventional tensiometer response versus time behavior and suction value. In the Second Part, approaches or semi-empirical models are proposed to estimate the variation of mechanical properties of unsaturated soils with respect to suction, which include: - Bearing capacity of unsaturated fine-grained soils - Variation of bearing capacity of unsaturated fine-grained soils with respect to matric suction - Variation of initial tangent elastic modulus of unsaturated soils below shallow foundations with respect to matric suction - Variation of maximum shear modulus with respect to matric suction for unsaturated non-plastic sandy soils (i.e. plasticity index, Ip = 0 %) In the Third Part, approaches (or methodologies) are suggested to simulate the vertically applied stress versus surface settlement behavior of shallow foundations in unsaturated coarse-grained soils assuming elastic-perfectly plastic behavior. These methodologies are extended to simulate the stress versus settlement behavior of both model footings and in-situ plates in unsaturated coarse-grained soils. The results show that there is a reasonably good comparison between the measured values (i.e. soil suction, bearing capacity, elastic and shear modulus) and those estimated using the techniques or models proposed in this thesis research. The models (or methodologies) proposed in this thesis research are promising and encouraging for modeling studies and practicing engineers to estimate the variation of mechanical behavior of unsaturated soils with respect to matric suction.

The properties of composite materials in which spherical inclusions are embedded in a matrix of some kind, have been studied for many decades and many analytical models have been developed which measure these properties. There has been a steady progression in the complexity of models over the years, providing greater insight into the nature of these materials and improving the accuracy in the measurement of their properties. Some of the properties with which this thesis is concerned are, the elastic, thermal and electrical properties of such composites. The size of the spherical inclusion which acts as the reinforcing phase, has a major effect on the overall properties of composite materials. Once an inclusion is embedded into a matrix, a third region of different properties between the inclusion and matrix is known to develop which is called the interphase. It is well known in the composite community that the smaller the inclusion is, the larger the interphase region which develops around it. Therefore, with the introduction of nanoparticles as the preferred reinforcing phase for some composites, the interphase has a major effect on its properties. It is the aim of this thesis to consider the role of the interphase on the properties of composites by modeling it as an inhomogeneous region. There is much scientific evidence to support the fact that the interphase has an inhomogeneous nature and many papers throughout the thesis are cited which highlight this. By modeling the inhomogeneous properties by arbitrary mathematical functions, results are obtained for the various properties in terms of these general functions. Some specific profiles for the inhomogeneous region are considered for each property in order to demonstrate and test the models against some established results.

Over the past 50 years, several advancements have been made in the research area of the mechanics of unsaturated soils. These advancements can be categorized into two groups; (i) development (or improvement) of testing techniques (or apparatus) to determine the mechanical properties of unsaturated soils and (ii) development of (numerical, empirical or semi-empirical) models to estimate the variation of mechanical properties of unsaturated soils with respect to suction based on the experimental results. Implementation of the mechanics of unsaturated soils in conventional geotechnical engineering practice, however, has been rather limited. The key reasons for the limited practical applications may be attributed to the lack of simple and reliable methods for (i) measuring soil suction in the field quickly and reliably and (ii) estimating the variation of mechanical properties of unsaturated soils with respect to suction. The main objective of this thesis research is to develop simple and reliable techniques, models or approaches that can be used in geotechnical engineering practice to estimate sol suction and the mechanical properties of unsaturated soils. This research can be categorized into three parts. In the First Part, simple techniques are proposed to estimate the suction values of as-compacted unsaturated fine-grained soils using a pocket penetrometer and a conventional tensiometer. The suction values less than 300 kPa can be estimated using a strong relationship between the compressive strength measured using a pocket penetrometer and matric suction value. The high suction values in the range of 1,200 kPa to 60,000 kPa can be estimated using the unique relationship between the initial tangent of conventional tensiometer response versus time behavior and suction value. In the Second Part, approaches or semi-empirical models are proposed to estimate the variation of mechanical properties of unsaturated soils with respect to suction, which include: - Bearing capacity of unsaturated fine-grained soils - Variation of bearing capacity of unsaturated fine-grained soils with respect to matric suction - Variation of initial tangent elastic modulus of unsaturated soils below shallow foundations with respect to matric suction - Variation of maximum shear modulus with respect to matric suction for unsaturated non-plastic sandy soils (i.e. plasticity index, Ip = 0 %) In the Third Part, approaches (or methodologies) are suggested to simulate the vertically applied stress versus surface settlement behavior of shallow foundations in unsaturated coarse-grained soils assuming elastic-perfectly plastic behavior. These methodologies are extended to simulate the stress versus settlement behavior of both model footings and in-situ plates in unsaturated coarse-grained soils. The results show that there is a reasonably good comparison between the measured values (i.e. soil suction, bearing capacity, elastic and shear modulus) and those estimated using the techniques or models proposed in this thesis research. The models (or methodologies) proposed in this thesis research are promising and encouraging for modeling studies and practicing engineers to estimate the variation of mechanical behavior of unsaturated soils with respect to matric suction.

Asphalt mixtures are complex composites that comprise aggregate, asphalt binder, and air. Several research studies have shown that the mechanical behavior of the asphalt mixture is strongly influenced by the matrix, i.e. the asphalt binder. Therefore, accurate constitutive models for the asphalt binders are critical to ensure accurate performance predictions at a material and structural level. However, researchers who use computational methods to model the micromechanics of asphalt mixtures typically assume that (i) asphalt binders behave linearly in shear, and (ii) either bulk modulus or Poisson’s ratio of asphalt binders is not time dependent. This research develops an approach to measure and model the shear and bulk behavior of asphalt binders at intermediate temperatures. First, this research presents the findings from a systematic investigation into the nature of the linear and nonlinear response of asphalt binders subjected to shear using a Dynamic Shear Rheometer (DSR). The DSR test results showed that under certain conditions a compressive normal force was generated in an axially constrained specimen subjected to cyclic torque histories. This normal force could not be solely attributed to the Poynting effect and was also related to the tendency of the asphalt binder to dilate when subjected to shear loads. The generated normal force changed the state of stress and interacted with the shear behavior of asphalt binder. This effect was considered to be an “interaction nonlinearity” or “three dimensional effect”. A constitutive model was identified to accommodate this effect. The model was successfully validated for several different loading histories. Finally, this study investigated the time-dependence of the bulk modulus of asphalt binders. To this end, poker-chip geometries with high aspect ratios were used. The boundary value problem for the poker-chip geometry under step displacement loading was solved to determine the bulk modulus and Poisson’s ratio of asphalt binders as a function of time. The findings from this research not only improve the understanding of asphaltic materials behavior, but also provide tools required to accurately predict pavement performance. / text