O avanço na tecnologia de hardware está permitindo que máquinas SMP de 2 a 8 processadores estejam disponíveis a um custo cada vez menor, possibilitando que a incorporação de tais máquinas em aglomerados de PC's ou até mesmo a composição de um aglomerado de SMP's sejam alternativas cada vez mais viáveis para computação de alto desempenho. O grande desafio é extrair o potencial que tal conjunto de máquinas oferece. Uma alternativa é usar um paradigma híbrido de programação para aproveitar a arquitetura de memória compartilhada através de multihreadeing e utilizar o modelo de troca de mensagens para comunicação entre os nós. Contudo, essa estratégia impõe uma tarefa árdua e pouco produtiva para o programador da aplicação. Este trabalho apresenta o sistema CPAR- Cluster que oferece uma abstração de memória compartilhada no topo de um aglomerado formado por nós mono e multiprocessadores. O sistema é implementado no nível de biblioteca e não faz uso de recursos especiais tais como hardware especializado ou alteração na camada de sistema operacional. Serão apresentados os modelos, estratégias, questões de implementação e os resultados obtidos através de testes realizados com a ferramenta e que apresentaram comportamento esperado. / The advance in hardware technologies is making small configuration SMP machines (from 2 to 8 processors) available at a low cost. For this reason, the inclusion of an SMP node into a cluster of PCs or even clusters of SMPs are becoming viable alternatives for high performance computing. The challenge is the exploitation of the computational resources that these platforms provide. A Hybrid programming paradigm which uses shared memory architecture through multihreading and also message passing model for inter node communication is an alternative. However, programming in such paradigm is very hard. This thesis presents CPAR- Cluster, a runtime system, that provides shared memory abstraction on top of a cluster composed by mono and multiprocessor nodes. Its implementation is at the library level and doesn't require special resources such as particular hardware or operating system moditfications. Models, strategies, implementation aspects and results will be presented.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The organotellurium compounds have been the subject of research due to their pharmacological and toxicological properties. It is believed that the main mechanism involved in the toxicity of these compounds is the ability to interact with sulfhydryl groups from molecules biologically active. Beyond the toxicological effects, some pharmacological properties have been attributed to organotellurium compounds. This study aimed to investigate the potential toxicological and pharmacologic of 2-phenylethynyl-butyltellurium (PEBT) through experiments in vitro and in vivo in rodents. To evaluate the toxicological effect the PEBT was used at different concentrations in the oxidation of mono and dithiols and analysis of enzyme Na+K+ -ATPase and lactate dehydrogenase in vitro. Furthermore, lethality studies were performed to calculate the LC50 LD50 of this compound and for better understanding their toxicity. To evaluate the pharmacological effect the PEBT was used at a dose of 1mg/kg 30 minutes before the behavioral experiments, evaluation of locomotor activity, forced swim test (FST) and the tail suspension test (TST), immediately after testing the cerebral cortex was removed for analysis of monoamine oxidase (MAO) enzyme. The results showed that the PEBT oxidized thiols of low molecular weight and inhibits the activity of the enzyme Na+K+ - ATPase by oxidation of sulfhydryl groups, and such oxidation is dependent on the tellurium atom in the structure of this compound. Moreover, the acute administration of PEBT showed an antidepressant-like effect on TNF in mice, as well inhibits the activity of MAO-A enzyme in the cerebral cortex, demonstrating the involvement of this enzyme in its antidepressant-like effect. / Os compostos orgânicos de telúrio têm despertado o interesse dos pesquisadores, devido as suas propriedades farmacológicas e toxicológicas. Acredita-se que o principal mecanismo envolvido na toxicidade desses compostos, seja a capacidade de interagir com os grupamentos sulfidrílicos de moléculas biologicamente ativas. Além dos efeitos toxicológicos, propriedades farmacológicas vêm sendo atribuídas aos compostos orgânicos de telúrio. Esse estudo teve como objetivo investigar o potencial toxicológico e farmacológico do 2- feniletinilbutil-telúrio (PEBT), através de experimentos in vitro e in vivo em roedores. Para a avaliação toxicológica, o PEBT foi utilizado em diferentes concentrações na oxidação de mono e ditiois e na determinação da atividade das enzimas Na+K+ -ATPase e lactato desidrogenase, in vitro. Ainda nesse sentido estudos de letalidade foram realizados para calcular a CL50 e DL50 desse composto para melhor compreender a sua toxicidade. Para a avaliação farmacológica, o PEBT foi administrado em camundongos, na dose de 1 mg/kg 30 minutos antes dos experimentos comportamentais, avaliação da atividade locomotora, teste do nado forçado (TNF) e teste de suspensão da cauda (TSC). Após os testes comportamentais, os animais foram mortos e o córtex cerebral foi retirado para determinação da atividade da enzima monoamina oxidase (MAO). Os resultados mostraram que o PEBT oxida tióis de baixo peso molecular e inibe a atividade da enzima Na+K+ -ATPase, pela oxidação de seus grupamentos sulfidrilicos, sendo essa oxidação depende da presença do átomo de telúrio na estrutura do composto. Além disso, a administração aguda do PEBT produz um efeito do tipo antidepressivo no TNF em camundongos, bem como inibe a atividade da enzima MAO-A em córtex cerebral, demonstrando o envolvimento dessa enzima no seu efeito do tipo antidepressivo.

Les lysophosphatidylcholines (LPCs), molécules amphiphiles, peuvent jouer le rôle d'un vecteur pour des acides gras d'intérêt. Ces molécules peuvent être synthétisées par voie enzymatique. Ce travail a pour objectif de synthétiser des LPCs contenants des acides gras mono ou poly insaturés par voie enzymatique. La sn-3-glycerophosphatidylcholine (GPC), l'acide oléique (C18 : 1, ω9), l'acide linoléique (C18 : 2, ω6) et l'acide docosahexènoïque (C22 : 6, ω3) ont été utilisés comme substrats. Ces synthèses ont été réalisées par estérification sans solvant en utilisant une lipase immobilisée. Du point de vue cinétique, une optimisation des conditions de la synthèse de l'oléoyl-LPC a été effectuée en étudiant chaque facteur d'une façon indépendante. Les conditions optimales sont : Lipozyme RM-IM (10% par rapport la masse totale des substrats), un rapport molaire de 20 (acide oléique /GPC), ni ajout ni élimination d'eau au milieu réactionnel, une température de 50°C et une agitation à 750rpm. Ces conditions permettent d'avoir 75% d'oléoyl-LPC en 24h. L'oléoyl-LPC a été purifiée sur une cartouche de silice et caractérisée par SM pour confirmer son identité. L'optimisation de la synthèse de linoléoyl-LPC a été faite via un plan d'expériences. C'est une approche qui permet de varier simultanément les paramètres étudiés. Parmi les 17 combinaisons étudiées, une combinaison a permis d'obtenir 93% de linoléoyl-LPC en 6h. Ce résultat a été confirmé en répétant la manipulation 4 fois. Comme l'oleoyl-LPC, la linoleoyl-LPC a été purifiée et caractérisée par SM. Des essais préliminaires ont montré que la synthèse de DHA-LPC est faisable. 30% de DHA-LPC a été obtenu au bout de 72h de réaction. Enfin, il faut noter que la quantité produite de di-acyl-PC était toujours faible par rapport à celle de l'acyl-LPC. Ce dernier résultat est expliqué par une apparition faible du phénomène de la migration du groupement acyle. / The Lysophosphatidylcholines (LPCs), amphiphilic molecules, can act as a carrier for fatty acids of interest. These molecules can be synthesized enzymatically. This work aims to synthesize LPCs containing mono or poly unsaturated fatty acids enzymatically. Sn-3-glycerophosphatidylcholine (GPC), oleic acid (C18: 1, 9ω), linoleic acid (C18: 2, 6ω) and docosahexaenoic acid (C22: 6, 3ω) were used as substrates. These syntheses were performed by esterification without solvent using an immobilized lipase. From kinetic point of view, optimization of conditions for synthesis of oleoyl-LPC was carried out by studying each factor independently. The obtained optimal conditions are : Lipozyme RM-IM (10% w/w), a molar ratio of 20 (oleic acid / GPC), neither addition or elimination of water was applied to the reaction mixture, a temperature at 50° C and stirring at 750rpm. These conditions allow to obtain 75% oleoyl-LPC within 24 hours. The oleoyl-LPC was purified on a silica cartridge and analyzed by MS to confirm its identity. The optimization of linoleoyl-LPC synthesis was made using a design of experiments. This is an approach that can simultaneously vary the parameters studied. Among the 17 combinations studied, one combination allows to obtain 93% of linoleoyl-LPC in 6 hours. This result was confirmed by repeating this manipulation 4 times. Like oleoyl-LPC linoleoyl-LPC was purified and characterized by MS. Preliminary trials have shown that the syntheis of DHA-LPC is feasible. 30% of DHA-LPC was obtained after 72h of reaction. Finally, it should be noted that the produced amount of di-acyl-PC is always low compared to that of the acyl-LPC. This latter is explained by a low appearance of the phenomenon of acyl migration.

Desenvolveu-se sistemática de trabalho para coletar valores da função ñ vs. concentração de ligante e processos de cálculo de constantes de equilíbrio. Os sistemas Co(II)/N-3 e Ni(II)/N-3 foram analisados com o objetivo de identificar os complexos formados e determinar as respectivas constantes de estabilidade. O estudo desses sistemas foi realizado a (25,0 ± 0,l) °C e em força iônica 2,0 M ajustada com NaClO4. Com base na formação de soluções tampão N-3/ HN3 e na variação de pH provocada pela adição do íon metálico Co(II) ou Ni(II), realizou-se um estudo potenciométrico para cada um dos sistemas acima indicados. Estes estudos foram realizados numa ampla faixa de concentração analítica de azoteto (0 a 2,000 M) e para diversas concentrações do íon metálico, para possibilitar uma análise mais detalhada sobre a formação dos complexos. Em relação ao sistema Co(II)/N-3 foi possível determinar a formação de cinco espécies mononucleares. As respectivas constantes de estabilidade foram calculadas por três métodos diferentes. A comparação dos desvios calculados para os valores de ñ obtidos a partir de cada um dos três conjuntos de constantes permitiu decidir pelo seguinte conjunto de constantes: β1 = 5,67 ± 0,20 M-1 β2 = 19,5 ± 2,1 M-2 β3 = 6,52 ± 4,66 M-3 β4 = 15,8 ± 2,0 M-4 β5 = 4,83 ± 0,31 M-5 Num comportamento diverso ao dos íons Co (II) o niquel levou à formação de complexos polinucleares. Realizou-se então um estudo monográfico envolvendo aspectos estruturais, qualitativos e quantitativos sobre a formação de complexos polinucleares. O sistema Ni(II)/-3 foi então analisado através do método potenciométrico citado mas em condições que permitiram a obtenção de um número maior de dados experimentais em relação ao sistema Co(II)/N-3, para tornar possível a determinação da composição assim como o cálculo das constantes de estabilidade das espécies existentes em equilíbrio. Dois métodos de cálculo foram utilizados, adotando-se o seguinte conjunto de constantes de equilibrio: mononucleares Ni(N3)+ β11 = 5,80 Ni(N3)2 β12 = 9,69 Ni(N3)-3 β13 = 7,54 Ni(N3)=4 β14 = 534 binucleares Ni2(N3)3+ β21 = 4,06 Ni2(N3)2+2 β22 = 31,8 Ni2(N3)+3 β23 = 175 Ni2(N3)4 β24 = 2,06x104 Ni2(N3)-5 β25 = 2,30x105 Ni2(N3)=6 β26 = 7,15x105 trinucleares Ni3(N3)2+4 β34 = 2,28x104 Ni3(N3)+5 β35 = 3,51x106 Ni3(N3)6 β36 = 1,17x108 Três estudos complementares foram realizados para o sistema Ni(II)/N-3. A utilização de métodos espectrofotométrico e potenciométrico em condições de excesso de metal em relação ao ligante permitiu determinar as contantes de estabilidade das espécies mono e binucleares mais simples: NiN+3 e Ni2N3+3. O método espectrofotométrico forneceu um valor de β11 = 6,90 M-1, enquanto que o segundo método forneceu β11 = 7,53 ± 0,10 M-1 e β21 = 4,29 ± 0,15 M-2. Medidas espectrofotométricas realizadas nas condições do segundo método permitiram calcular as absortividades molares das duas espécies, em cinco comprimentos de onda na faixa do ultravioleta. No comprimento de onda de absorção máxima dos complexos (293 nm) os valores de ε encontrados foram os seguintes: ε11 = 362 e ε21 = 475 cm-1mol-11. Finalmente, um estudo condutométrico feito em alta concentração de Ni(II) (0,4 M) e para concentrações variáveis de azoteto permitiu identificar a formação da espécie Ni2(N3)-5. Fez-se uma série de diagramas de distribuiçao, os quais mostraram as contribuições de cada espécie em função do ligante e, eventualmente, da concentração total do íon metálico (sistemas polinucleares). / A systematic approach in order to obtain ñ data vs. free ligand concentration was developed as well as calculation procedures for treatment of the data to obtain stability constants. The Co(II)/N-3 and Ni(II)/N-3 systems were analysed with the purpose to identify the formed complexes and to compute its stability constants. The equilibria studies were performed at 25.0 ± 0.1° C and 2.0 M ionic strength maintained with NaClO4. The pH change in azide/hydrazoic acid buffers caused by the metal cation addition was the basis of the potentiometric method to study the complex formation. A wide range of azide concentration was used at several metal cation (cobalt or nickel) concentration in order to provide reliable results. With regard to the Co(II)/N-3 system the formation of five stepwise complexes was detected. The stability constants were computed from three different calculation procedures. The best set of formation constants is: β1 = 5,67 ± 0,20 M-1 β2 = 19,5 ± 2,1 M-2 β3 = 6,52 ± 4,66 M-3 β4 = 15,8 ± 2,0 M-4 β5 = 4,83 ± 0,31 M-5 The nickel(II)/azide system presented a quite different behaviour as compared with the cobalt(ll) one, as extensive polynuclear complex formation is attained. A monographic study about this kind of complex formation seemed interesting to be included in this thesis. An extensive study of the nickel(II)/azide system was performed at conditions wich provide enough experimental data to be treated on basis of polynuclear complex formation. Two different calculation procedures lead to the following set of equilibrium constant: mononuclear Ni(N3)+ β11 = 5,80 Ni(N3)2 β12 = 9,69 Ni(N3)-3 β13 = 7,54 Ni(N3)=4 β14 = 534 binuclears Ni2(N3)3+ β21 = 4,06 Ni2(N3)2+2 β22 = 31,8 Ni2(N3)+3 β23 = 175 Ni2(N3)4 β24 = 2,06x104 Ni2(N3)-5 β25 = 2,30x105 Ni2(N3)=6 β26 = 7,15x105 trinuclears Ni3(N3)2+4 β34 = 2,28x104 Ni3(N3)+5 β35 = 3,51x106 Ni3(N3)6 β36 = 1,17x108 Two complementary studies, potentiometric and spectrophotometric, were performed at high metal cation/1igand ratio in order to create conditions for the existence of on1y a few species as Ni(N3)+ and Ni2(N3)3+. β11 from spectrophotometric method is 6.90 M-1; from potentiometric method β11 is 7.53 ± 0.10 M-1 and β21 is 4.29 ± 0.15 M-2. The molar absorptivities at λmax, 293 nm, was calcu1ated as ε11 = 362 cm-1. mol-1.1 and ε21 = 475 cm-1. mol-1.1. Finally a conductometric titration of 0.4 M niekel(II) with 3.9 M sodium azide showed a high contribution of Ni2(N3) -5 in agreement with the potentiometric study. A series of distribution diagrarns were plotted in order to have the percent contribution of each species vs. the ligand concentration and, eventually, vs. the metal concentration when polynuclear complexes are formed.

This dissertation presents a search for dark matter particles produced in association with a Z boson in proton-proton collisions. The dataset consists of 139 fb^{-1} of collision events with centre-of-mass energy of 13 TeV, and was collected by the ATLAS detector from 2015-2018 at the Large Hadron Collider. Signal region events are required to contain a Z boson that decays leptonically to either e^+e^- or μ^+μ^-, and a significant amount of missing transverse momentum, which indicates the presence of undetected particles. Two types of dark matter models are studied: (1) simplified models with an s-channel axial-vector or vector mediator that couples to dark matter Dirac fermions, and (2) two-Higgs-doublet models with an additional pseudo-scalar that couples to dark matter Dirac fermions. The main Standard Model background sources are ZZ, WZ, non-resonant l^+l^-, and Z+jets processes, which are estimated using a combination of data and/or simulation. A new reweighting technique is developed for estimating the Z+jets background using γ+jets events in data; the resulting estimate significantly improves on the statistical and systematic errors compared to the estimate obtained from simulation. The observed data in the signal region are compared to Standard Model prediction using a transverse mass discriminant distribution. No significant excess in data is observed for the simplified models and two-Higgs-doublet models studied. A statistical analysis is performed and several exclusion limits are set on the parameters of the dark matter models. Results are compared to direct detection experiments, the CMS experiment, and other ATLAS searches. Prospects and improvements for future iterations of the search are also presented. / Graduate

Ce travail porte sur le développement d'un procédé de fabrication de transistor monoélectronique {single-electron transistor, SET). II dresse un portrait de l'état de l’art actuel et met en lumière un manque dramatique de marge d'opération dans le fonctionnement de ces dispositifs. Cette problématique est présentée comme une des limites majeures aux espoirs de développement commercial de cette technologie. La thèse propose d'aller chercher la marge de manoeuvre manquante par un procédé de fabrication qui exploite le contrôle des dimensions verticales des dispositifs. Les résultats montreront que si les approches actuelles 2D semblent avoir atteint leurs limites physiques, l'approche 3D permet d'accéder à des températures d'opération encore insoupçonnées jusqu'à présent. L'impact est important, puisqu'une analyse de la thèse conclura que, même en tenant compte des pires fluctuations du procédé de fabrication, les SETs issus du concept 3D conservent une marge d'opération appréciable. La gamme de température d'opération obtenue est semblable aux transistors à effet de champ conventionnels (field effect transistor, FET) et on peut penser qu'il serait ainsi possible d'utiliser les deux technologies simultanément sur un même substrat afin de créer de nouvelles fonctionnalités issues de cette technologie hybride SET/FET.

This thesis starts with an introduction to Simulataneous Localization and Mapping (SLAM) and more background on Visual SLAM (VSLAM). The goal of VSLAM is to map the world with a camera, and at the same time localize the camera in that world. One important step is to optimize the acquired map, which can be done in several different ways. In this thesis, two state-of-the-art optimization algorithms are identified and compared, namely the g2o package and the SBA package. The results show that SBA is better on smaller datasets, and g2o on larger. It is also discovered that there is an error in the implementation of the pinhole camera model in the SBA package.

Phthalates are industrial plasticizers with a wide range of applications. Di-(2-ethylhexyl) phthalate (DEHP) is one of the most highly produced and frequently studied phthalates. Its metabolite, mono-(2-ethylhexyl) phthalate (MEHP) is known as a testicular toxicant. The objective of this study was to examine expression of the genes of interest in testicular germ cells exposed to MEHP in a dose- and time-dependent manner at concentrations of 1µM, 10µM, and 100µM at 24, 48, 72 and 96hr time points. The genes consisted of Testisin, GSPT1, and MGMT genes which are a tumor suppressors, phase II xenobiotic metabolizing enzyme and DNA repair gene respectively. These genes were analyzed by Quantitative Real Time PCR (RT-PCR). The results revealed an overall down-regulation for each gene as the concentration and/or time increased. Testisin was the focus of the gene expression analysis. Testisin is epigenetically silenced in testicular germ cell tumors (TGCT) by DNA methylation at the 5’CpG island of the gene. To investigate if MEHP is capable of DNA hypermethylation, a co-exposure with 5-azacytidine (demethylating agent) was conducted. Compared with the 5-azacytidine treatment alone, there was a significant down-regulation of the Testisin gene in the co-exposure. This suggests that MEHP may down-regulate Testisin gene expression by DNA methylation. These findings provide evidence that MEHP can alter the expression of Testisin, GSTP1 and MGMT, genes that are associated in the risk of developing testicular germ cell tumors. In addition, results indicated that MEHP may cause DNA methylation leading to the down-regulation/silencing of genes such as Testisin.

This master-thesis presents an approach to track and count the number of fruit incommercial mango orchards. The algorithm is intended to enable precision agri-culture and to facilitate labour and post-harvest storage planning. The primary objective is to develop an multi-view algorithm and investigate how it can beused to mitigate the effects of visual occlusion, to improve upon estimates frommethods that use a single central or two opposite viewpoints. Fruit are detectedin images by using two classification methods: dense pixel-wise cnn and regionbased r-cnn detection. Pair-wise fruit correspondences are established between images by using geometry provided by navigation data, and lidar data is used to generate image masks for each separate tree, to isolate fruit counts to individual trees. The tracked fruit are triangulated to locate them in 3D space, and spatial statistics are calculated over whole orchard blocks. The estimated tree counts are compared to single view estimates and validated against ground truth data of 16 mango trees from a Bundaberg mango orchard in Queensland, Australia. The results show a high R2-value of 0.99335 for four hand labelled trees and a highest R2-value of 0.9165 for the machine labelled images using the r-cnn classifier forthe 16 target trees.

Cientes de que o histórico, a intensidade e a expansão espacial das atividades antrópicas afetam a resiliência e as taxas de recuperação de um ecossistema florestal, existe atualmente o desafio de determinar qual ou quais métodos de restauração ecológica serão os mais eficientes em termos de recursos em metas para cada local. Isso porque existem diversos tipos de métodos e estratégias de restauração ecológica, os quais variam desde apenas assistir um ecossistema se reestabelecer à alta intervenção as quais mudarão os rumos da sucessão ecológica. Neste contexto, o presente trabalho busca analisar se a nucleação aplicada, um método de restauração florestal de baixo custo e intervenção na área, restaurou e ocupou um antigo pasto abandonado numa área de preservação permanente dentro do campus da Escola Superior de Agricultura \"Luiz de Queiroz\". Para isso, foi implantado um experimento de nucleação aplicada, vizinho a um fragmento de floresta estacional semidecidual em maio de 2008 onde foram plantados 40 núcleos. Foram testadas 4 espécies (Inga laurina, Cordia trichotoma, Albizia niopoides e Jacaratia spinosa) com dez repetições cada e com apenas uma espécie por núcleo. Os núcleos foram então mensurados anualmente até 2013 e posteriormente no ano de 2016. Mensuramos o diâmetro na altura do peito (DAP), altura, área basal, biomassa, índice de área de vegetação (via fotografias hemisféricas) e área de copa (calculado como uma elipse). Depois, em 2017 alocamos 24 parcelas de 2 metros de raio no entorno dos núcleos para avaliar a densidade, riqueza e diversidade dos indivíduos regenerantes. Na análise dos dados comparamos os diferentes tratamentos através de análises de variância e dos testes de comparações de média de tukey para o último período de medição nos diferentes parâmetros dendrométricos. Procurando entender se alguma espécie plantada em núcleos monoespecíficos conseguiu atrair maior quantidade de regenerantes, espécies e diversidade comparamos entre os diferentes tratamentos esses parâmetros. Posteriormente, realizamos um modelo linear generalizado a fim de encontrar possíveis relações entre densidade e a riqueza dos indivíduos regenerantes com as variáveis dos núcleos, como altura máxima, área basal, índices de área de vegetação e área de copa. Os resultados encontrados nos dizem que existem diferenças no desenvolvimento dos diversos parâmetros analisados incluindo a ocupação da área pelas diferentes espécies plantadas na nucleação aplicada. Encontramos também para o presente projeto que a densidade de regenerantes no entorno dos núcleos é significantemente relacionada com a área basal e altura das árvores dos núcleos e a riqueza de espécies dos indivíduos em regeneração é significantemente relacionada com apenas a área área basal das árvores presentes nos núcleos. Sugerimos o uso de nucleação aplicada para restaurar áreas com grau médio de degradação usando uma espécie pioneira com altas taxas de crescimento. Os núcleos monoespecíficos devem ser utilizados apenas em paisagens com abundância de propágulos para que não haja baixa diversidade de espécies a médio prazo. / Despite the history that intensity and spatial expansion of anthropogenic activities affect the resilience and recovery rates of a forest ecosystem, we have the current challenge in forest restoration to determine which ecological restoration methods will be most efficient for each specific area, this in terms of resources and goals. In this context, the ecological restoration of degraded ecosystems must be specific to each site. The present work aimed to analyze applied nucleation, a method of low cost and low intervention in the area of forest restoration, occupied an abandoned pasture in a permanent preservation area inside the campus \"Luiz de Queiroz\" College of Agriculture in Piracicaba, São Paulo. For this, an applied nucleation experiment was implanted next to a semideciduous seasonal forest fragment in May 2008, where 40 nuclei were planted. Four species (Inga laurina, Cordia trichotoma, Albizia niopoides and Jacaratia spinosa) were tested with ten replicates each, with only one specie per nuclei. The nuclei were then measured annually until 2013 and later in 2016. In the individual\'s data, we measured the diameter at the chest height (DBH), height, basal area, biomass, vegetation area index (via hemispheric photographs) and Crown area (calculated as an ellipse). After that, in 2017, 24 subplots were allocated around the nuclei to evaluate the density, specie richness and diversity of regenerating individuals. In order to compare the different treatments used, we performed analysis of variance and the Tukey mean comparison test for the different dendrometrical parameters between species. Later, in order to understand the facilitation of density and specie richness by the different treatments of nuclei, we related the height, basal area, vegetation area index and area with these parameters through a generalized linear model. The results show that there are differences in the development of the various parameters analyzed including the occupation of the area by the different species planted in the applied nucleation. We also found for the present project that the density of regenerants found near the nuclei are significantly related to the sum of basal area and height of the nuclei and the species richness of regenerants are significantly related to the sum of the basal area of the individuals\' nuclei. We suggest the use of applied nucleation to restore areas with a medium degree of degradation using a pioneer species with high grown rates. The mono specific nuclei should be use just only in landscapes with plenty of propagules. So that there is no low diversity of species in the medium term.