Coastal water quality is intricately linked to environmental factors, particularly meteorological and hydrological conditions. Weather conditions determine wind speed and direction, which in turn influence coastal currents, and these currents play a critical role in the transport and variability of water quality parameters. Understanding how these dynamic interactions impact water quality is essential for the effective management and preservation of coastal ecosystems. This dissertation investigates the effects of varying flow conditions on key water quality parameters in the Louisiana Continental Shelf, utilizing data from two research cruises. The study specifically focuses on how concentration variability and transport mechanisms of water quality indicators respond to different environmental conditions, including the passage of cold fronts and periods of non-frontal conditions. In this research, data were collected using a conductivity-temperature-depth (CTD) system and through the analysis of water samples to assess changes in nutrient concentrations, dissolved oxygen levels, chlorophyll-a, and Colored Dissolved Organic Matter (CDOM). These samples were collected under two distinct environmental conditions: following a cold front and during non-frontal conditions. Analytical methods were used to quantify the water quality parameters, while hydrodynamic modeling was applied to assess the transport and dispersion patterns of these indicators. The results show that cold fronts increase nutrient concentrations and decrease dissolved oxygen levels due to enhanced mixing and upwelling. The cold front activity also promotes southwestward transport of water quality parameters, driven by the region's hydrodynamic forces. Under stable weather conditions, nutrient and chlorophyll-a concentrations increase, but transport is limited, with reduced dispersion resulting from weaker hydrodynamic forces. CDOM component analysis revealed that cold front conditions lower CDOM concentrations and improve light penetration, while non-frontal conditions elevate CDOM concentrations, reducing light penetration and impacting aquatic ecosystem productivity. These findings enhance our understanding of how meteorological events, hydrological conditions, and water quality interact in coastal regions. The research demonstrates how weather conditions distinctly influence nutrient dynamics and CDOM behavior. These insights are crucial for developing effective water quality management strategies for the Louisiana Continental Shelf and similar coastal regions worldwide, supporting global efforts to mitigate challenges posed by climate change and human activity in coastal ecosystems.

Made available in DSpace on 2017-07-25T19:30:49Z (GMT). No. of bitstreams: 1 Allison Jose Fornari.pdf: 1614252 bytes, checksum: 1528c75fb76d8cae0fcfb0d22642bbe1 (MD5) Previous issue date: 2014-07-31 / Nitrogen (N) and potassium (K) are nutrients required in larger amounts by corn, requiring adequate levels of N and K phases of higher demand culture. This study aimed to evaluate the effects of fertilization with N and K nutrition, performance and productivity of maize in long-term tillage system. The experiment was carried out on a Yellow Red Oxisol dystrophic with 530 g kg-1 clay under no-till for 30 years. The sequence of crops adopted before the establishment of the experiment was oat/corn/wheat/soybean/wheat/soybean with the annual crop of oat/corn in 33% of the area and wheat/soybean in 67% of the area. A randomized complete block design was used, with three replications in a split-plot arrangement. The treatments consisted of applications of urea at 0, 75, 150, and 225 kg N ha-1 to subplots within plots with KCl previously at 0, 100, 200, and 300 kg K ha-1. The K rates were applied on the surface before seeding, and the N rates were applied in top dressing when corn plants were at the V4 stage (four fully expanded leaves). The Ca/K, Ca + Mg/K, and √ relationships in the topsoil (0-0.20 m) were linearly reduced with increasing rates of K applied pre-sowing. There was no significant effect of increasing K rates on concentrations of N, P, K, Ca, Mg, S, Mn, Zn, Cu and Fe in corn leaves, dry weight of shoots and grains of corn. The increase in N rates increased the contents of N, P, K, Ca, Mg, S, Mn and Zn in the leaves of corn at the silking stage, increased the concentration of N and decreased the content of P, K, Mn, Zn, and Fe in the aboveground part and increased the concentrations of N, P, K, S, Mn, Zn, and Fe in corn grains. The increasing rates of K increased the extraction of K, while the N rates increased the extraction of N, P, K, Ca, Mg, S, Mn, Zn, and Cu by corn shoots. The export of N, P, K, Ca, Mg, S, Mn, Zn, Cu, and Fe was not affected by K rates, but increased with increasing rates of N applied in corn. Potassium fertilization increased plant height and ear height. Increasing N rates increased stem diameter, plant height and ear height, the dry matter production of shoots, the number of kernels per row and per spike, spike and the mass of the mass 1000 grains. Potassium rates did not significantly influence corn grain yield, while increasing rates of N reached double the corn yield. The N use efficiency decreased with increasing N rates in corn and has not improved with potassium fertilization. The relative grain yield of corn was closely related to foliar N, P, Mg and S. The relative grain yield of corn had closer relations with the products N × P, N × K, and N × S than with N/P, N/K, and S/N ratio in leaf tissue of corn. The results suggest that the high demand of N by corn grown after oat under no-till does not increase the demand for K fertilization in corn, in soil with sufficient content of exchangeable K in the 0-0.20 m layer. / O nitrogênio (N) e o potássio (K) são os nutrientes exigidos em maiores quantidades pela cultura do milho, sendo necessárias quantidades adequadas de N e K nas fases de maior demanda da cultura. O presente trabalho teve o objetivo de avaliar os efeitos da adubação com N e K na nutrição, no desempenho e na produtividade da cultura do milho em sistema plantio direto de longa duração. O experimento foi realizado em um Latossolo Vermelho Amarelo distrófico com 530 g kg-1 de argila, conduzido em sistema de plantio direto por 30 anos. A sequência de culturas adotada antes da instalação do experimento foi aveia/milho/trigo/soja/trigo/soja, com cultivo anual de aveia/milho em 33% da área e de trigo/soja em 67% da área. O delineamento experimental empregado foi em blocos ao acaso, em esquema de parcelas subdivididas, com três repetições. Nas parcelas foram aplicados 100, 200 e 300 kg ha-1 de K, além de um tratamento controle sem K. Nas subparcelas foram empregados 75, 150 e 225 kg ha-1 de N, além de um tratamento controle sem N. As doses de K foram aplicadas na forma de KCl, a lanço em pré-semeadura, e as de N foram aplicadas na forma de ureia, em cobertura, quando as plantas de milho estavam no estádio V4 (quatro folhas completamente expandidas). As relações Ca/K, Ca+Mg/K e √ no solo (0–0,20 m) foram linearmente reduzidas com o aumento das doses de K aplicadas em pré-semeadura. Não houve efeito significativo do aumento das doses de K sobre as concentrações de N, P, K, Ca, Mg, S, Mn, Zn, Cu e Fe nas folhas de milho, na massa seca da parte aérea e nos grãos do milho. O incremento de doses de N aumentou os teores de N, P, K, Ca, Mg, S, Mn e Zn nas folhas de milho na fase de florescimento, aumentou a concentração de N e diminuiu os teores de P, K, Mn, Zn e Fe na matéria seca da parte aérea, e aumentou as concentrações de N, P, K, S, Mn, Zn e Fe nos grãos de milho. O incremento das doses de K aumentou a extração de K, enquanto as doses de N aumentaram a extração de N, P, K, Ca, Mg, S, Mn, Zn e Cu pela parte aérea do milho. A exportação de N, P, K, Ca, Mg, S, Mn, Zn, Cu e Fe não foi alterada pelas doses de K, porém aumentou com o incremento de doses de N aplicadas na cultura do milho. As doses de K aumentaram a altura das plantas e de inserção da espiga. O aumento das doses de N aumentou o diâmetro de colmo, a altura das plantas e de inserção da espiga, a produção de matéria seca da parte aérea, o número de grãos por fileira e por espiga, a massa da espiga e a massa de 1000 grãos. As doses de K não influenciaram significativamente a produtividade de grãos de milho, enquanto que o aumento das doses de N chegou a dobrar a produtividade de grãos do milho. A eficiência de utilização de N diminuiu com o incremento das doses de N aplicadas em cobertura e não melhorou com adubação potássica na cultura do milho. A produtividade relativa de grãos de milho esteve estreitamente relacionada com os teores foliares de N, P, Mg e S. A produtividade relativa de grãos de milho apresentou relações mais estreitas com os produtos N × P, N × K e N × S do que com as relações N/P, N/K e N/S no tecido foliar do milho. Os resultados sugerem que a alta demanda de N pelo milho cultivado após aveia preta no sistema plantio direto não aumenta a demanda de K na adubação do milho, em solo com teor suficiente de K trocável na camada de 0–0,20 m.

This doctoral thesis deals with the biogeochemical processes taking place in estuarine systems known as 'rias', located at the NW of the Iberian Peninsula. Its realization has been possible thanks to financial support from the JAE-Program of the Spanish Council for Scientific Research (CSIC) co-funded by the European Social Fund (ESF), and the ‘INTERESANTE' project, funded by the CICYT. Additionally, it is a contribution to the Spanish LOICZ program (Land-Ocean Interactions in the Coastal Zone). An oceanographic characterization of the Northern Galician Rias is presented in Chapter 2. This chapter also involves an extensive analysis of summer upwelling and downwelling processes, winter shelf-water upwelling, and poleward intrusion in the northern Galician shelf. Chapter 3 deals with the fluvial contributions to the Northern Galician Rias, including the quantification of major and minor chemical elements (nutrient salts, trace elements) in the dissolved and particulate matter, chlorophyll-a, DIN, PON, DOC, POC and C/N ratio. In Chapter 4 the sediment-water fluxes of nutrients in a ria coastal systems are discussed. Fluxes driven by molecular diffusion and advective transport across sediment-water interfaces during tidal flooding for the Northern Galician Rias are calculated. Chapter 5 focuses on the hydrological, chemical and biological interactions in the Northern Galician Rias, exemplified by the case of the Ria of Barqueiro, identifying their oceanographic periods in relation to nutrient availability, primary production, phytoplankton biomass, taxonomic composition and hydrographic patterns.

<p>Storm water is defined as runoff from precipitation such as rain or snow. It is collected in sewage disposal systems and since it mainly originates from urban areas it can contain high levels of heavy metals, nutrients and oil etc. Polluted storm water is currently treated by different methods such as wet ponds, constructed wetlands and filter strips. This study investigates these methods, which in earlier studies have shown to give varying results regarding their treatment efficiency.</p><p>This thesis has been written on commission of SWECO VIAK and was aimed at studying those parameters that may affect the treatment efficiency of nutrients and heavy metals in storm water treatment facilities (STF:s). Through literature studies the dominating treatment processes in ponds and wetlands were examined. The flow pattern was then simulated in four different storm water ponds, of varying shape and size, by using a physically based computer model. The residence time distribution for each pond could then be calculated as well as the hydraulic efficiency, which is a measure on how well the inflowing water is spread throughout a pond. Finally a multiple regression analysis was performed. This analysis examined how the reduction of lead, copper, zinc and cadmium depends on the following factors: the specific pond area, the variance of the residence time distribution, the hydraulic efficiency and the concentration of incoming metals and suspended solids. The specific pond area is a factor which is defined as the pond area divided by the reduced run off area.</p><p>The results from the multiple regression analysis indicate that an increasing content of inflowing suspended solids leads to a better treatment efficiency regarding lead and copper. The specific area showed to have some effect on the treatment efficiency, but this effect was less than that of the content of inflowing suspended solids. The reduction of cadmium depended mainly on the specific area and the incoming concentration of cadmium. An increasing reduction of zinc could to some extent be explained by an increasing specific area.</p><p>The results from this analysis and earlier studies are emphasizing the fact that adsorption to particles and sediment is one of the main processes for a good separation of heavy metals from storm water. Longer measurements series from additional treatment facilities are needed to build a statistical model which, in the future, can be used to predict the treatment efficiency of storm water ponds.</p> / <p>Dagvatten är regn- och smältvatten som samlas upp i avloppssystem. Då detta vatten huvudsakligen härstammar från urban bebyggelse så kan det innehålla höga halter tungmetaller, närsalter och olja med mera. Idag behandlas förorenat dagvatten bland annat genom nyttjande av dammar, våtmarker och översilningsytor. Dessa behandlingsalternativ har dock visat sig ge varierande resultat med avseende på dess reningseffektivitet.</p><p>Detta arbete har utförts på uppdrag av konsultföretaget SWECO VIAK och syftade till att studera vilka parametrar som påverkar reningseffekten av närsalter och tungmetaller i dagvattendammar. Genom litteraturstudier undersöktes de dominerande reningsprocesserna i dammar och våtmarker. Därefter modellerades strömningen i 4 dammar med varierande form och storlek. Vid detta arbete erhölls en fördelning av uppehållstiden hos vattnet för respektive damm samt ett värde på den hydrauliska effektiviteten, vilken är ett mått på hur väl det inströmmande vattnet sprids ut i dammen. Slutligen utfördes en multipel regressionsanalys.</p><p>Vid denna analys undersöktes hur reningen av bly, koppar, zink och kadmium beror av den specifika dammarean, variansen hos vattnets uppehållstidsfördelning, den hydrauliska effektiviteten samt inkommande metall- och TSS-koncentration (suspenderat material). Den specifika dammarean är en faktor vilken defineras som kvoten mellan dammens area och dess reducerade avrinningsområde.</p><p>Resultaten från den multipla regressionen tyder på att en ökad inkommande halt av suspenderat material till dammen har störst betydelse för en förbättrad avskiljning av bly och koppar. Även den specifika arean och metallkoncentrationen i inflödet visade sig ha en viss, men något mindre, betydelse. Reduktionen av kadmium berodde däremot till störst del av den specifika arean och inkommande kadmiumkoncentration. En ökad reduktion av zink förklarades till viss del av en ökad specifik area. Resultatet att halten av suspenderat material i hög grad avgör reningen samt tidigare studier understryker det faktum att adsorption till partiklar och sediment verkar vara en av de viktigaste processerna för en god avskiljning av tungmetaller. Längre mätserier från fler dagvattendammar krävs dock för att i framtiden ta fram en statistisk modell vilken skulle kunna användas till att prediktera reningseffekten.</p>

<p>In the process of making carton boards into Skoghall Mill, are great quantities of water being contaminated. This water has to be treated before it is possible to release into the recipient, Kattfjorden. One of the contaminations that is important to extract from the wastewater, is organic carbon (measured as TOC-total organic carbon) because microbiological breakdown can lead to the bottom becomig free of oxygen.</p><p>The wastewater treatment at Skoghall Mill is done through an external wastewater treatment that is located at the factory. The biological treatment (aerated lagoon) is a 140 000 m3 water pool with surface aerator, which is give oxygen to the water. In the lagoon live microorganisms who performs the biggest part of the TOC-reduction.</p><p>One problem with the lagoon is that the treatment of TOC is considerably worse during wintertime than during summertime. The reduction is during wintertime only 40 %, while during summertime it is about 70-80 %. This seasonal variation can to a large extent be explained by, that the water temperature in the lagoon decreases wintertime. The low water temperature does that the biological activity in the lagoon is reduced, which reduces the TOC-reduction. A second reason can also be that the microorganisms do not have enough bioavailable nutrients that is needed for growth of a new biomass.</p><p>In this thesis the need of nutrient has been examined. In two test pools, addings of nutrient were being done, to come to the conclusion of a good dosage of nutrient which would lead to a larger reduction of TOC, without enlarged quantities of nitrogen/phosphorus in the outgoing water. One of the test pools hold a summer temperature (30°C), and the other test pool hold a winter temperature (10°C). The test was performed this way to see if there would be any difference in the need of nutrient between summertime and wintertime.</p><p>The most important conclusion was that a nutrient dosage to the Skoghall aerated lagoon, rise the TOC-reduction wintertime. It was also concluded that it was foremost the nitrogen that contributed to the raised TOC-reduction, and nitrogen is thereby considered to be the growth restraining element wintertime. It seemed like phosphorus had been overdosed during the tests, while enlarged halts of the substance could be seen in the outgoing water, and an optimal dosage of phosphorus could not be done. The recommendation that came as a results from the tests, was that during wintertime add nitrogen and phosphorus according to TOC:N:P-quota 100:0,75:0,10. To the summer pool was no considerable improvement of the TOC-reduction seen, as a result of the nutrient addings. It is also no reason to dosage nutrients in summertime.</p> / <p>Vid tillverkning av kartong och pappersmassa på Skoghalls Bruk förorenas stora mängder vatten, som därmed måste renas före utsläpp till recipienten, Kattfjorden, en vik i Vänern. En av de föroreningar som är viktig att avlägsna ur avloppsvattnet är organiska kolföreningar (TOC-total organic carbon) eftersom det vid mikrobiologisk nedbrytning av TOC åtgår syre vilket kan leda till att sjöbotten blir syrefri.</p><p>Rening av avloppsvatten på Skoghall Bruk sker genom en extern reningsanläggning som är placerad på bruket. Det biologiska reningssteget i reningsanläggningen är en s.k. luftad damm. Detta är en 140 000 m3 stor vattenbassäng med ytluftare som syresätter vattnet. I dammen lever mikroorganismer vilka utför den största delen av TOC-reduktionen i brukets reningsanläggning.</p><p>Ett problem med dammen är att reningen av TOC är betydligt sämre vintertid än sommartid. Reduktionen av det till dammen inkommande TOC, är vintertid endast ca 40 % medan den sommartid ligger kring 70-80 %. Denna årstidsvariation kan till stor del förklaras av att vattentemperaturen i dammen sjunker vintertid. Den låga temperaturen medför att den biologiska aktiviteten i dammen sjunker, vilket hämmar TOC-reduktionen. En annan orsak till en låg TOC-reduktion kan vara att det för mikroorganismerna råder brist på biotillgängliga närsalter (kväve och fosfor) som behövs för tillväxt av ny biomassa.</p><p>I detta examensarbete har det undersökts behovet av närsalter för att höja TOC-reduktionen. I två laborationsdammar (ca 20 l) skedde doseringar av närsalter i syfte att finna en bra doseringskvot som innebar en ökad reduktion av TOC, utan att det blev förhöjda kväve-/fosforhalter i utgående vatten. En av laborationsdammarna höll sommartemperatur (ca 30°C) och en höll vintertemperatur (ca 10°C) vilket gjordes för att undersöka om det var skillnad av närsaltsbehovet mellan sommar och vintertid.</p><p>Den viktigaste slutsatsen av försöken var att en närsaltsdosering till Skoghalls luftade damm höjer TOC-reduktion vintertid. Det konstaterades att det var kvävet som främst bidrog till den ökade TOC-reduktionen och kväve anses därmed vara det tillväxtbegränsande ämnet vintertid. Fosfor tycktes ha överdoserats under försöken, då det sågs förhöjda halter i utgående vatten, och en optimal dosering av fosfor kunde inte avgöras. Den rekommendation som föll ut av de laborativa försöken var att vintertid dosera kväve och fosfor enligt TOC:N:P-kvot 100:0,75:0,10. Till sommardammen sågs inte någon förbättring av TOC-reduktionen till följd av närsaltstillsatserna, vilket innebär att närsaltsdoseringar sommartid är obefogat. Vidare sågs tendenser till att slammets sedimentationsegenskaper, hos framförallt vinterdammen, blev bättre till följd av närsaltsdosering, men brist på mätdata medför att det är svårt att dra slutsatser om det verkligen varit så.</p>

A hydrologic model and watershed export model was used to estimate the loading of nutrients and total suspended solids from un-gaged local watersheds associated with Lake Texoma. Discharge to the reservoir from local watersheds was predicted using a modification of the curve number method in HEC Hydrologic Modeling System (HEC-HMS). Event mean concentrations were applied to land use to estimate loadings of nutrients and TSS. Total discharge from the local watersheds was estimated to be 3.02 x 107 cubic meters for a study window of March 1 to May 31, 1997, less than 10 percent of the input to the lake from the Red River and Washita River systems. Loadings were estimated to be 33,553 kg nitrogen, 4,401 kg phosphorus, and 3,423,140 kg TSS. The models and results obtained from their application appear to have potential utility for use in a water quality management decision support system for the reservoir.

I compared and contrasted nitrogen and phosphorus concentrations and land use differences in two oligotrophic lakes (Sooke and Shawnigan) and two meso-eutrophic lakes (St. Mary and Elk) in order to evaluate nutrient concentrations over time, and evaluate the relationship between in-lake nutrients and land use in the surrounding watershed. I used MapShed© nutrient transport modeling software to estimate the mass load of phosphorus and nitrogen to each lake, and evaluated the feasibility of land use modifications for reducing in-lake nutrients. In comparing nitrogen and phosphorus data in Sooke and Shawnigan Lakes, I determined that natural watershed characteristics (i.e., precipitation, topography, and soils) did not account for the elevated nutrient concentrations in Shawnigan verses Sooke Lake. Natural watershed characteristics indicated that external loads into Shawnigan Lake would be lesser-than or equal to those into Sooke Lake if both watersheds were completely forested. I evaluated trends of in-lake nutrient concentrations for Sooke and Shawnigan Lakes, as well as two eutrophic lakes, St. Mary and Elk. Ten to 30-year trends indicate that nitrogen and phosphorus levels in these lakes have not changed significantly over time. Time-segmented data showed that nutrient trends are mostly in decline or are maintaining a steady-state. Most nutrient concentration data are not precipitation-dependent, and this, coupled with significant correlations to water temperature and dissolved oxygen, indicate that in-lake processes are the primary influence on lake nutrient concentrations -- not external loading. External loading was estimated using, MapShed©, a GIS-based watershed loading software program. Model validation results indicate that MapShed© could be used to determine the effect of external loading on lake water quality if accurate outflow volumes are available. Based on various land-cover scenarios, some reduction in external loading may be achieved through land-based restoration (e.g., reforestation), but the feasibility of restoration activities are limited by private property. Given that most of the causal loads were determined to be due to in-lake processes, land-based restoration may not be the most effective solution for reducing in-lake nitrogen and phosphorus concentrations. / Graduate

With the world’s population set to reach 9 billion by the mid 21st century food security has never been more important. Increased competition regarding land for agricultural use and over fished seas means it falls to aquaculture to meet the global demands for protein requirements. The largest supply of aquaculture products are cultivated in South East Asia where the industry has seen rapid expansion, particularly of pond production in the past 50 years. This initial expansion has come at a cost with mangrove losses and eutrophication of natural water sources resulting. The impact of these not only affects other stakeholders, including domestic users, but effects will be felt by the aquaculture industry. Indiscriminate release of effluents to the surrounding water reduces the water quality for other users and may impact on the farm discharging the water originally. Poor water quality can then result in poor growth rates and increased mortalities reducing the profitability of the farm and endangering the livelihood of the farmer. If aquaculture is to meet the global food demand it is important that current and future enterprises are developed with sustainability at the fore front. This study investigates the nutrient dynamics in pond culture in South East Asia, focussing initially on four countries outlined by the SEAT (Sustainable Ethical Aquaculture Trade) project, including Thailand, Vietnam, China and Bangladesh. Within the four countries the main species cultured for export were identified resulting in tilapia, shrimp, pangasiid catfish and prawn. Following a farmer survey designed to collect a large volume of data over a range of topics including, water management, social, economic and ethical perceptions, dynamic models were developed, using Powersim Studio 8© (Powersim, Norway), for a generic fish and shrimp ponds separately. The models draw on data from the survey combined with other literature sources to provide outputs for Total Nitrogen and Total Phosphorus in water and sediment as well as dissolved oxygen in the pond water. One of the biggest challenges facing this study was the objective selection of relevant sites for case studies to apply the models to. With such a large preselected set of sites (200 per species per country) it was important that the method be capable of handling such large datasets. Thusly it was decided that a multivariate method be used due to the removal of any pre judgement of the data relevant to the study. In order to investigate the nutrient dynamics water management data was used in the multivariate analysis to identify any similarity between the practices occurring on farms. The case studies in this project focus on Thailand and Vietnam, covering tilapia, shrimp and pangasius. Prawn farms were disregarded as, through the survey, it was discovered most production was for domestic trade. The models were adapted to each farm case study expanding the boundary from pond level to farm level, providing an output for each pond in terms of nutrients in the water and production levels and the farm as a whole for dissolved oxygen and sediment accumulation. The results of the models suggest the culture species to be taking up much of the TN added followed by the accumulation in sediments in shrimp ponds, while TP is mostly taken up by sediments. The fish case studies suggest that most of the TN is discharged to the environment followed by uptake. While Total phosphorus shows similar results to shrimp, accumulating in the sediment. The models presented in this study can be used to estimate outputs from farms of similar water management strategies and can assist in the determination of where improvements can be made to reduce the potential for eutrophication of natural water sources.

Dans les pays en voie de développement, les évolutions prévues par les organismes internationaux montrent une progression beaucoup plus importante des maladies chroniques non transmissibles, comme le cancer, que des maladies transmissibles. Actuellement, la majorité des études montre que la tendance à l'augmentation de l'incidence et de la mortalité par cancer colorectal est plus marquée dans les sociétés riches que dans les sociétés pauvres. Dans les pays développés le cancer colorectal est au deuxième rang en ce qui concerne la mortalité par cancer et les changements dans les habitudes alimentaires et le mode de vie sont souvent mis en cause dans son développement. Bien que les données épidémiologiques soient rares, les populations de la plupart des pays asiatiques ne sont pas conscientes du risque grandissant que peut constituer pour eux le cancer colorectal. Comme les autres pays en développement, le Cambodge n'a pas de système d'enquête de consommation alimentaire, ni de système de surveillance ou de contrôle des substances chimiques, ni de système d'enregistrement, de contrôle ou de dépistage des cancers. L'objectif de cette recherche était de débuter la première observation d'une relation entre régime alimentaire et cancer colorectal au Cambodge. L'idée générale était d'identifier les aliments grands contributeurs des régimes alimentaires de la population cambodgienne, et de les traduire en calories, nutriments et composés bioactifs en se fondant sur les bases de données existantes. Une étude a également été menée sur les comportements de cette population, en ce qui concerne ses habitudes alimentaires et les modes de préparation des aliments susceptibles de produire des substances cancérigènes telles que des amines hétérocycliques (AH) et du benzo[a]pyrene (BaP). Pour réaliser ce travail de recherche, une enquête de consommation alimentaire a été effectuée à l'aide d'un rappel sur 24 heures et d'un questionnaire de fréquence alimentaire, afin de créer une base de données dédiée pour servir à l'évaluation des risques. Ensuite, une évaluation de l'exposition aux AH et BaP a été effectuée, afin d'établir une hiérarchie du risque que ces substances posent pour cette population d'étude ; celle-ci a été réalisée en croisant les données de consommation alimentaire, obtenues par le biais d'une enquête de consommation alimentaire individuelle exécutée dans ce travail, avec les données de contamination rassemblées à partir des analyses chimiques reportées dans la littérature scientifique récente. Les résultats ont été comparés avec les valeurs toxicologiques de référence. Ils ont montré que les habitudes alimentaires au Cambodge n'ont pas encore changé pour s'adapter à l'alimentation occidentale, et qu'elles offrent plus de composés protecteurs. L'exposition aux contaminants néoformés (AH et BaP) a été plus faible par rapport aux pays régionaux ainsi qu'aux pays développés. La présence de composés endogènes tels que glucides, fibres alimentaires, calcium et vitamine C semble protéger la population cambodgienne du cancer colorectal. Des recherches supplémentaires sont nécessaires pour étudier les interactions entre l'alimentation, le mode de vie et les facteurs génétiques, ainsi que d'autres facteurs également. / From the projection in the future of international bodies, non-communicable diseases such as cancer may increase more than communicable diseases in developing countries. The highlight of this burden may be due to the changing of dietary patterns and lifestyle. Currently, the evolution of food consumption, consumption pattern and colorectal cancer is very worrying worldwide. It is a disease in economically ‘developed' populations, and it is the second killer among other cancers; however, its incidence seems lower in poor countries. As the other developing countries, Cambodia has no system of food survey, no monitoring and control system of chemical substances, and also, there is no control and registration system for cancer. Thus, the objective of this research was to start the first observation of the relationship between dietary pattern and colorectal cancer in Cambodia. The general idea of the study was to identify the dietary patterns, and larger contributors to colorectal cancer in Cambodia, then to translate them into calories, nutrients and bioactive compounds, based on the existing database. Another goal was to assess the difference of dietary habit and cooking methods in the studied population that could lead to the production of colorectal carcinogens such as heterocyclic amines (HAs) and benzo[a]pyrene (BaP). In order to create the food consumption database needed for food risk assessment, a food consumption survey was conducted using 24-hour recall and food frequency questionnaire. A dietary assessment of HAs and BaP has been done, in order to establish a hierarchy of the importance of the risk that these substances represent to the health of this population. Dietary exposures to HAs and BaP were obtained by combining food consumption data, obtained from the individual food survey specially designed and carried out during this research, with the contamination data gathered from chemical analysis reported in the recent literature. The observation results have been compared with the toxicological reference values. The results show that dietary patterns in Cambodia have not changed yet to adapt to the Western diet, and contain higher levels of protected nutrients. The exposure to neoformed contaminants (HAs and BaP) was lower than the values reported among other Asian countries, and lowest as compared to the developed countries. The presence of endogenous compounds such as carbohydrates, dietary fibers, calcium and vitamin C, seems to protect the Cambodian population from colorectal cancer. Further research is needed to study the interaction of diet, lifestyle and the genetic background, and other factors as well.

Ce travail de thèse a pour but d'étudier la fixation d'azote marine dans les environnements riches mais déficitaires en azote (N), comparé au phosphore (P) dans un rapport N:P<16, grâce à l'utilisation d'approches complémentaire en culture in vitro et sur le terrain in situ. La première partie de ce travail a consisté à évaluer la réponse de la fixation d'azote de la cyanobactérie unicellulaire diazotrophe Crocosphaera watsonii face à des concentrations micromolaires en azote inorganique dissous (DIN) supposées inhiber l'activité de fixation d'azote : - suite à un apport sporadique, ou, - après une longue période d'acclimatation. Les résultats de ces études n'ont pas permis d'observer une inhibition des activités de fixation d'azote de cet organisme, laissant supposer que ce processus pourrait être actif dans une zone de l'Océan ayant ces même caractéristiques biogéochimiques : le Sud Est Tropical de l'Océan Pacifique (ETSP). En effet, cette zone est une des trois plus grandes zones de minimum d'oxygène (OMZ) de l'Océan et d'intenses processus de pertes de N (dénitrification et anammox) y ont lieu, résultant en un déficit de N par rapport au P. Des études présumaient que des processus inverses, de gains de N par la fixation d'azote, pourraient y être actifs mais aucune mesure à l'échelle du bassin n'y avait été faite car la fixation d'azote n'était supposée se produire que dans les environnements oligotrophes, comme les gyres subtropicaux. Dans le cadre d'un projet international, des missions océanographiques ont pu avoir lieu dans cette zone en Février 2010 pendant un évènement El Niño et en Mars-Avril 2011 pendant un évènement La Niña. / The objectif of these thesis was to study dinitrogen fixation in marine environments rich but deficient of nitrogen (N) compared to phosphorus (P) in a ratio N:P<16, by using complementary approaches in culture in vitro and in the field in situ. The first part of this work was to evaluate the response of nitrogen-fixing unicellular Cyanobacteria Crocosphaera watsonii faced with micromolar concentrations of dissolved inorganic nitrogen (DIN) supposed to inhibit nitrogen fixation activity : - after sporadic input, or, - after a long period of acclimatization. The results of these studies have failed to observe the inhibition of nitrogen fixation activities of this organism, suggesting that this process could be active in an area of the Ocean with these same biogeochemical characteristics : the Eastern Tropical South Pacific (ETSP). Indeed, this area is one of the three largest oxygen minimum zones (OMZ) of the Ocean, where intense processes of N losses (denitrification and anammox) took place, resulting in a deficit of N compared to P. Studies assumed that the inverse process, gain of N by nitrogen fixation, could be active in the ETSP but no measurements across the basin have been performed because nitrogen fixation was assumed to occur only in oligotrophic environments, such as the subtropical gyres. In the framework of an international project, cruises took place in this area in February 2010 during a El Niño event and in March-April 2011 during a La Niña event. Results of these two cruises have confirmed that nitrogen fixation was unexpectedly active with an intensity comparable to those reported in oligotrophic areas.