FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / Objetivou-se por meio deste estudo caracterizar a composiÃÃo bioquÃmica do plasma seminal e dos parÃmetros seminais de coelhos criados no NE do Brasil, identificando ainda as variaÃÃes que possam ocorrer ao longo do ano. O experimento foi conduzido nas instalaÃÃes do Setor de Cunicultura do Departamento de Zootecnia/UFC. O Ãndice de conforto tÃrmico dentro da instalaÃÃo foi calculado de acordo com a fÃrmula do ITU, modificada para coelhos. Foram utilizados 20 coelhos da raÃa Nova ZelÃndia Branca, criados em sistema flat-deck, alimentados com raÃÃo comercial. Os ejaculados foram coletados duas vezes por semana (outubro/2009 a setembro/2010), utilizando-se uma vagina artificial. Em seguida, os ejaculados foram avaliados quanto ao volume, cor, aspecto, vigor, motilidade e concentraÃÃo espermÃtica, apÃs foram centrifugados e o PS (plasma seminal) foi removido e acondicionado em tubos eppendorfs a -18 oC. Para realizaÃÃo das anÃlises bioquÃmicas foi feito um pool mensal de PS de cada animal, onde foram avaliadas as concentraÃÃes de glicose, frutose, colesterol total e proteÃnas totais. Neste estudo, os parÃmetros seminais foram influenciados significativamente (p<0,05) pelo mÃs de coleta, independente da existÃncia de estresse pelo calor. Na avaliaÃÃo dos parÃmetros seminais pela cor foi verificado que os ejaculados de cor branca perolada foram representativos da boa qualidade do sÃmen. Quanto ao aspecto, os melhores resultados foram encontrados nos ejaculados com aspecto cremo-leitoso. No que se refere Ãs caracterÃsticas bioquÃmicas, o aspecto do ejaculado influenciou significativamente (p<0,05) somente as concentraÃÃes de frutose e glicose. Foram constatadas diferenÃas significativas das concentraÃÃes de frutose entre os diferentes meses do ano. A glicose foi identificada como um constituinte, comumente encontrada no PS. A concentraÃÃo de colesterol total no PS de coelhos no presente experimento foi mais baixa que o encontrado por outros autores. O estudo das correlaÃÃes de Pearson demonstrou associaÃÃes significativas (baixas e moderadas), positivas e negativas entre os parÃmetros seminais e os bioquÃmicos. Os parÃmetros bioquÃmicos apresentaram correlaÃÃes entre si. Nesse estudo foi encontrada correlaÃÃo negativa da concentraÃÃo espermÃtica com a frutose. Os resultados permitiram concluir demonstram que os coelhos criados no nordeste do Brasil sÃo perfeitamente adaptados, pois apresentam caracterÃsticas seminais e bioquÃmicas normais, apesar da existÃncia de estresse tÃrmico. / This study was carried out to characterize the biochemical composition of seminal plasma and semen parameters of rabbits reared in Northeast Brazil, also identifying the changes that occur throughout the year. The experiment was conducted at the Sector of the Rabbit Department of Animal Science / UFC. The index of thermal comfort inside the facility was constructed according to the formula of the THI, modified for rabbits. The ejaculates were obtained twice a week from 20 New Zealand White rabbits, reared in flat-deck system (October/2009 to September/2010). After collection, the semen was measured and evaluated for volume, color, appearance, vigor, sperm concentration and motility. To obtain the SP, the semen was centrifuged and then stored at - 18ÂC. To perform the biochemical analysis was made a monthly pool of PS of each animal. The samples were tested according to the concentrations of fructose, glucose, total cholesterol and total protein. In this study, semen parameters were affected significantly (p <0.05) by sampling month, despite the presence of heat stress. In the evaluation of semen parameters was found that the color of the ejaculates was pearly white representative of good semen quality. Regarding the aspect, the best results were found in ejaculates with cream-milky appearance. With regard to biochemical characteristics, the aspect of the ejaculate significantly (p <0.05) only the concentrations of fructose and glucose. Significant differences in concentrations of fructose among the different months of the year. Glucose was identified as a constituent, usually found in the PS. Significant differences in concentrations of fructose among the different months of the year. Glucose was identified as a constituent, usually found in the PS. The study of the Pearson correlations showed significant associations (low and moderate) positive and negative differences between the semen parameters and biochemical. In this study we found a negative correlation with sperm concentration of fructose. The results showed that rabbits show created in Northeastern Brazil are perfectly adapted, as seminal and biochemical characteristics have normal, despite the existence of heat stress.

The aim of this paper is to estimate the influence of different breeds on rabbit growing and physiological indicator. The temperature of our investigated rabbits varied from 37.6 ºC to 38.8 ºC when the environmental temperature was 18 ºC. Good dental health and a perfect 'bite' help to assimilate feeds nutrients. The fur of all rabbits was clean, sleek and not matted. The average weight varied from 3.7 kg to 4.05 kg. Referring to various Lithuanian and foreign sources it turns out that there is no fixed hematological standards subjected to different breeds. Usually it is said that the quantity of cholesterol is 0.14-1.86 mmol/l, glucose – 2.78-5.18 mmol/, triglycerides – 1.38 mmol/. The state of health can be evaluated according to rabbits’ behavior. The author (Solar, 2001) makes out the main behaviour observation stages. With reference to these guidelines we have estimated that New Zealand rabbits’ eating and drinking in 1-hour period during the day made 20%, moving – 10%, rest – 70 %, no cecotrophy was observed. If to compare French Giant rabbits the periods were 33%, 37 % and 30 %. Conclusions:1. The rabbit growing has increased 22.8% during the last three years and the number of a herd of breeding rabbit has increased 13.7%. Italy provides about 40.7% and Spain 20.3% of rabbit meat for world market. 2. The White New Zealand and French Giant rabbits that were fed on industrial crops were healthy and blood index was within the mark. 3. The quantity of glucose, cholesterol... [to full text]

ALENCAR, Jôsy Maria Arruda de. Parâmetros seminais e composição bioquímicas do plasma seminal de coelhos criados no nordeste do Brasil. 2011. 54 f. : Dissertação (mestrado) - Universidade Federal do Ceará, Centro de Ciências Agrárias, Departamento de Zootecnia, Fortaleza-CE, 2011 / Submitted by Nádja Goes (nmoraissoares@gmail.com) on 2016-08-01T15:29:48Z No. of bitstreams: 1 2011_dis_jmaalencar.pdf: 450822 bytes, checksum: 81c939f6286bffdd6e44ed9ba0e39500 (MD5) / Approved for entry into archive by Nádja Goes (nmoraissoares@gmail.com) on 2016-08-01T15:30:03Z (GMT) No. of bitstreams: 1 2011_dis_jmaalencar.pdf: 450822 bytes, checksum: 81c939f6286bffdd6e44ed9ba0e39500 (MD5) / Made available in DSpace on 2016-08-01T15:30:03Z (GMT). No. of bitstreams: 1 2011_dis_jmaalencar.pdf: 450822 bytes, checksum: 81c939f6286bffdd6e44ed9ba0e39500 (MD5) Previous issue date: 2011 / This study was carried out to characterize the biochemical composition of seminal plasma and semen parameters of rabbits reared in Northeast Brazil, also identifying the changes that occur throughout the year. The experiment was conducted at the Sector of the Rabbit Department of Animal Science / UFC. The index of thermal comfort inside the facility was constructed according to the formula of the THI, modified for rabbits. The ejaculates were obtained twice a week from 20 New Zealand White rabbits, reared in flat-deck system (October/2009 to September/2010). After collection, the semen was measured and evaluated for volume, color, appearance, vigor, sperm concentration and motility. To obtain the SP, the semen was centrifuged and then stored at - 18°C. To perform the biochemical analysis was made a monthly pool of PS of each animal. The samples were tested according to the concentrations of fructose, glucose, total cholesterol and total protein. In this study, semen parameters were affected significantly (p <0.05) by sampling month, despite the presence of heat stress. In the evaluation of semen parameters was found that the color of the ejaculates was pearly white representative of good semen quality. Regarding the aspect, the best results were found in ejaculates with cream-milky appearance. With regard to biochemical characteristics, the aspect of the ejaculate significantly (p <0.05) only the concentrations of fructose and glucose. Significant differences in concentrations of fructose among the different months of the year. Glucose was identified as a constituent, usually found in the PS. Significant differences in concentrations of fructose among the different months of the year. Glucose was identified as a constituent, usually found in the PS. The study of the Pearson correlations showed significant associations (low and moderate) positive and negative differences between the semen parameters and biochemical. In this study we found a negative correlation with sperm concentration of fructose. The results showed that rabbits show created in Northeastern Brazil are perfectly adapted, as seminal and biochemical characteristics have normal, despite the existence of heat stress. / Objetivou-se por meio deste estudo caracterizar a composição bioquímica do plasma seminal e dos parâmetros seminais de coelhos criados no NE do Brasil, identificando ainda as variações que possam ocorrer ao longo do ano. O experimento foi conduzido nas instalações do Setor de Cunicultura do Departamento de Zootecnia/UFC. O índice de conforto térmico dentro da instalação foi calculado de acordo com a fórmula do ITU, modificada para coelhos. Foram utilizados 20 coelhos da raça Nova Zelândia Branca, criados em sistema flat-deck, alimentados com ração comercial. Os ejaculados foram coletados duas vezes por semana (outubro/2009 a setembro/2010), utilizando-se uma vagina artificial. Em seguida, os ejaculados foram avaliados quanto ao volume, cor, aspecto, vigor, motilidade e concentração espermática, após foram centrifugados e o PS (plasma seminal) foi removido e acondicionado em tubos eppendorfs a -18 oC. Para realização das análises bioquímicas foi feito um pool mensal de PS de cada animal, onde foram avaliadas as concentrações de glicose, frutose, colesterol total e proteínas totais. Neste estudo, os parâmetros seminais foram influenciados significativamente (p<0,05) pelo mês de coleta, independente da existência de estresse pelo calor. Na avaliação dos parâmetros seminais pela cor foi verificado que os ejaculados de cor branca perolada foram representativos da boa qualidade do sêmen. Quanto ao aspecto, os melhores resultados foram encontrados nos ejaculados com aspecto cremo-leitoso. No que se refere às características bioquímicas, o aspecto do ejaculado influenciou significativamente (p<0,05) somente as concentrações de frutose e glicose. Foram constatadas diferenças significativas das concentrações de frutose entre os diferentes meses do ano. A glicose foi identificada como um constituinte, comumente encontrada no PS. A concentração de colesterol total no PS de coelhos no presente experimento foi mais baixa que o encontrado por outros autores. O estudo das correlações de Pearson demonstrou associações significativas (baixas e moderadas), positivas e negativas entre os parâmetros seminais e os bioquímicos. Os parâmetros bioquímicos apresentaram correlações entre si. Nesse estudo foi encontrada correlação negativa da concentração espermática com a frutose. Os resultados permitiram concluir demonstram que os coelhos criados no nordeste do Brasil são perfeitamente adaptados, pois apresentam características seminais e bioquímicas normais, apesar da existência de estresse térmico.

"June, 2008". / Thesis (PhD)--Macquarie University, Division of Environmental & Life Sciences, Dept. of Chemistry & Biomolecular Sciences, 2009. / Bibliography: p. 227-259. / Literature review -- Materials and methods -- Sampling sites and sampling material -- Enrichment cultures and molecular analyses -- Optical and binding characterisation of the QDs -- Applications of the QDs -- Concluding remarks. / White island, the most active volcano in New Zealand, is a poorly studied environment that represents an ideal site for the investigation of acidophilic thermophiles. The microorganisms present on here are continually exposed to extreme environmental conditions as they are surrounded by steamy sulphurous fumaroles and acidic streams. The sediment temperature ranges from 38°C to 104°C whilst maintaining pH values below 3. A survey of the volcanic hydrothermal system of White Island was undertaken in order to gain insights onto the microbial diversity using culture-dependant techniques and molecular and phylogenetic analyses. A novel liquid medium based on "soil-extract" was designed which supported growth of bacterial and archaeal mixed cultures. Molecular analyses revealed that the dominant culturable bacterial species belong to the Bacteroidetes, Firmicutes and α-Proteobacteria groups. Several previously uncultured archaeal species were also present in the mixed cultures. The knowledge gained from these studies was intended to help in the development of a novel microbial detection technique suitable for community analysis. -- Conventional molecular techniques used to study microbial biodiversity in environmental samples are both time-consuming and expensive. A novel bead-based assay employing Quantum dots (QDs) was considered to have many advantages over standard molecular techniques. These include high detection speeds, sensitivity, specificity, flexibility and the capability for multiplexed analysis. QDs are inorganic semiconductor nanoparticles made up of crystals about the size of proteins. It has been claimed that the physical and chemical properties of the QDs have significant advantages compared to organic dyes, including brighter fluorescence and resistance to photo-bleaching. Their optical properties facilitate the simultaneous imaging of multiple colours due to their flexible excitation and narrow band emission. Functionalised QDs are able to bind to different biological targets such as DNA, allowing high-throughput analysis for rapid detection and quantification of genes and cells. -- The optical and physical characteristics of the QDs as well their interaction with biomolecules are shown to be suitable for the development of a novel bead-based technique able to target the key microbial species and identify them by flow cytometric measurements (FCM). The broad absorption and narrow emission spectra of the QDs, as well as their fluorescence intensity and specify to target biomolecules, was compared to other organic fluorophores. The potential advantages and limitations of QDs as a fluorophores for biological applications are discussed. -- The data acquired during this study provides a broad overview of the microbial diversity and ecology of the volcanically-active hydrothermal systems of White Island and constitutes the baseline for the development of a novel bead-based technique based on QDs. / Mode of access: World Wide Web. / xvii, 259 p. ill. (some col.)

Die Implantation von Atemwegstents stellt eine therapeutische Option zur Behandlung maligner und benigner Stenosierungen des Tracheobronchialsystems dar. Es handelt sich um ein effektives Verfahren das in spezialisierten Zentren und nach strenger Indikationsstellung durchgeführt werden sollte. Die Bildung von Granulationsgewebe stellt dabei eine der häufigsten Komplikationen dar. In der vorliegenden Arbeit wurden ballonexpandierbare bare-metal stents (BMS) und drug-eluting stents (DES) histologisch und immunhistochemisch untersucht.  Die zellulären und extrazellulären Gewebereaktionen der zentralen Atemwege wurde in New Zealand White Rabbits (3,1 kg bis 4,8 kg) nach Explantation von sieben bare-metal stents (Bx Sonic, Johnson & Johnson Cordis, USA) und vier Sirolimus-eluting stents (Cypher Select, Johnson & Johnson Cordis, USA) untersucht. Die Implantationszeit betrug 12 Monate. Die Implantation  metallischer Stents in die zentralen Atemwege verursachte ähnliche Gewebereaktionen wie nach koronarer Stentimplantation. Die Entzündungsreaktionen waren vergleichsweise stärker ausgeprägt. Nach 12 Monaten konnte bei beiden Stenttypen neugebildetes Gewebe auf der luminalen Seite der Implantate gesehen werden. Dieses war durchsetzt von Entzündungszellen, fibromuskulären Zellen, Kollagenfasern, elastischen Fasern und neu gebildeten Kapillaren. In zwei Stentpräparaten konnten Chondrozyten im neugebildeten Gewebe gesehen werden. Fibromuskuläre Zellen färbten positiv für Smooth Muscle Actin  und Vimentin. Vereinzelt wurde dieses Granulationsgewebe von mehrschichtigem Epithel mit kubisch-polygonalen Zellen, entsprechend einer Epithelmetaplasie, bedeckt. Nicht in die Bronchialwand eingewachsene Stentstreben wurden von Mukus, Granulozyten, Makrophagen und azellulärem Detritus bedeckt. Das Flimmerepithel zeigte sich im gestenteten Bereich deutlich reduziert. Beide Stenttypen zeigten eine vergleichbare Entzündungsreaktion. Granulozyten als Zeichen einer akuten Entzündungsreaktion konnten im neugebildetem Gewebe und an der direkten Stentoberfläche gesehen werden. Weiterhin umgaben Makrophagen und fibromuskuläre Zellen eingewachsene Stentstreben, Lymphozyten und Makrophagen schlossen sich in der unmittelbaren Umgebung an. Einige Lymphozyten wurden als B-Lymphozyten (CD79 +) identifiziert. Fremdkörperriesenzellen wurden in drei Sirolimus-freisetzenden Stentpräparaten und einem bare-metal Stentpräparat gesehen.