Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-12-01T11:55:38Z No. of bitstreams: 2 Dissertação - Bruna dos Santos Rodrigues - 2013.pdf: 1797836 bytes, checksum: b56e1b3bdd63eeb9040d2ef050f157f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-12-04T14:18:47Z (GMT) No. of bitstreams: 2 Dissertação - Bruna dos Santos Rodrigues - 2013.pdf: 1797836 bytes, checksum: b56e1b3bdd63eeb9040d2ef050f157f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-12-04T14:18:47Z (GMT). No. of bitstreams: 2 Dissertação - Bruna dos Santos Rodrigues - 2013.pdf: 1797836 bytes, checksum: b56e1b3bdd63eeb9040d2ef050f157f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-08-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The death mechanisms induced by a new synthetic compound (4-FTC) in adenocarcinoma prostate cells (PC-3) and its toxicity were investigated in this study. PC-3 cells cytotoxity was evaluated by MTT reduction assay. The mechanisms involved in PC-3 death and cell cycle were investigated by flow cytometry and colorimetric assays. The compound toxicity was analized by cytotoxicity of mononuclear cells (MTT reduction assay) and 3T3 cells (neutral red uptake assay), myelotoxicity, haemolytic activity and acute oral toxicity. 4-FTC has concentration dependent cytotoxic activity in PC-3 cells, and 184,6 μM IC50. Investigation of death mechanisms indicated death by apoptosis, because of the significant increase in phosphatidylserine externalization (109,83%), loss of mytochondrial membrane potential (41,96%), significant increase of DNA fragmentation (284,02%) and capases 3/7 and 9 activity increase, 13,12% and 12,8%, respectively. Furthermore, the treatment of PC-3 cells wih 4-FTC did not induce the reactive oxygen species production, as well as, the induction of acid autophagic vesicles generation and did not change the cell cycle significantly. Althought 4-FTC was able to modulate the expression of some proteins that regulate cell cycle, incresead the expression of p53, p21 and p27. Thus, the results suggests that 4-FTC induced PC-3 death by apoptosis dependent by mitochondrial pathway activation. In toxicity evaluation, 4-FTC presented 52,86 μM and 19,63 μM IC50 to mononuclear and 3T3 cells, respectively; 27,35 μM IC50 to hematopoietic precursors; low acute oral toxicity, classified in GHS category 5, and not significant haemolytic activity. / Neste trabalho, investigaram-se os mecanismos de morte induzidos por um novo composto sintético, 4-fluorbenzaldeidotiossemicarbazona (4-FTC), nas células de adenocarcinoma prostático PC-3 e alguns parâmetros da toxicidade desse composto. A citotoxicidade nas células PC-3 foi avaliada pelo método de redução do MTT, método colorimétrico para avaliar a viabilidade celular. A investigação dos mecanismos de morte induzidos foi realizada por citometria de fluxo e ensaio colorimétrico. A toxicidade do composto foi avaliada pela citotoxicidade em células mononucleares pelo método de redução do MTT, citotoxicidade em células 3T3 pelo método de incorporação do vermelho neutro, mielotoxicidade, potencial hemolítico e toxicidade oral aguda. Os resultados obtidos mostram atividade citotóxica concentração dependente, com CI50 de 184,6 μM para PC-3. A investigação dos mecanismos de morte induzidos indicou morte por apoptose, pois houve aumento significativo da externalização da fosfatidilsserina (109,83%), perda do potencial de membrana mitocondrial em 41,96%, aumento significativo da fragmentação de DNA (284,02%) e aumento de caspases 3/7 e 9, em 13,12% e 12,8%, respectivamente. Além disso, não induziu a produção de espécies reativas de oxigênio, bem como, a formação de vesículas autofágicas ácidas e não alterou o perfil do ciclo celular de forma significativa. Embora tenha modulado a expressão de proteínas reguladoras do ciclo celular, aumentando a expressão de p53, p21 e p27. Assim, pode-se sugeririr que o 4-FTC induz morte por apoptose por meio de mecanismos de ativação dependentes da via mitocondrial em células PC-3. Na avaliação da toxicidade, o 4-FTC apresentou concentração inibitória 50% (CI50) de 52,86 μM e 19,63 μM para células mononuclerares e células 3T3, respectivamente; CI50 de 27,35 μM para precursores hematopoiéticos; baixa toxicidade oral aguda, sendo classificado na categoria 5 e baixo potencial hemolítico.

Aim of this Diploma thesis was to create a device capable of receiving navigational data from GPS. These data are subsequently stored in fixed memory and after connection with the PC are displayed it on the satellite map. The device was realized by using FPGA and GPS module LEA-5s. Description was created in the VHDL language, which was implemented into the circuit. The part of VHDL design was description of PICOBLAZE processor that controls whole system. For displaying and archiving data stored in device was created PC application GPS TRACER. It is able to display stored trace on the satellite map by using Google maps server. For created device were designed and manufactured PCBs, which were manually fitted.

Main goals of this work are to present game development process and game engine Unity3D and develop game server with application programming interface including framework for using the server. The first part of this work is tageted on history of game genres and game development process. The second part of the thesis is dedicated to describing Unity3D game engine with focus on Unity3D networking. Development tools used in implementation and server desing included framework for use of the server are described in the third part of the thesis. In the last chapter of this work are described most important classes used in developed application.

The goal of this thesis is to describe the issue of computer game creation and to develop a race game for people connected to the Virtual World using Unity 3D game engine. The first part of this thesis is dedicated to the history of video games, platforms and to describing of development processes and development tools. The next part is about Unity 3D game engine, which was used for creating the game. The resulting application is described in the last part.

This project deals with the PC diagnostics, creation of SW and HW audits and development of information system for storage and analysis of data gained. The system is designed for Microsoft Windows operation system. Part of the system called Client is developed in C/C++ programming language. To gather diagnostic data, the WMI interface is used. The web part of information system is created using PHP scripting language and database MySQL server.

京都大学 / 新制・課程博士 / 博士(工学) / 甲第24294号 / 工博第5067号 / 新制||工||1791(附属図書館) / 京都大学大学院工学研究科社会基盤工学専攻 / (主査)教授 高橋 良和, 教授 山本 貴士, 准教授 古川 愛子 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

International Telemetering Conference Proceedings / October 26-29, 1998 / Town & Country Resort Hotel and Convention Center, San Diego, California / The Space Sensing & Vehicle Control Branch of the Air Force Research Laboratory and Voss Scientific, Albuquerque, NM, are developing an advanced PC and COTS-based satellite telemetry processing, analysis and display system known as the PC-Satellite Telemetry Server (PC-STS). This program grew out of a need to develop less expensive, more capable, more flexible, and expandable solutions to the satellite telemetry analysis requirements of the Air Force. Any new system must employ industry standard, open architecture, network and database protocols allowing for easy growth and migration to new technologies, as they become available. Thus, the PC-STS will run on standard personal computers and the Windows NT operating system. The focus of this work and this paper is the Telemetry Server component, and in particular, the custom-built decommutation board. The decommution board will be capable of processing frame formatted and CCSDS packet telemetry. It will be capable of fully decommutating telemetry data, converting raw data to engineering units, and providing this data to the Telemetry Server host. Time tagged engineering units or minor frames of telemetry will be transmitted to the Telemetry Server processor via on-board memory buffers. The decom board uses the PCI bus, programmable DSPs, considerable on-board memory, and a SCSI bus for local archiving. This paper presents the general architecture of the PC-STS, and discusses specific design considerations. These include trade-offs made during the design of the board’s hardware and software, operational specifications, and graphical user interfaces to program, monitor, and control the board.

International Telemetering Conference Proceedings / October 28-31, 1996 / Town and Country Hotel and Convention Center, San Diego, California / In the last few years there has been a proliferation of GPS receivers and receiver manufacturers. Couple this with a growing number of DoD test programs requiring high accuracy Time-Space-Position-Information (TSPI) with diminishing test support funds and/or needing a wide area, low altitude or surface tracking capability. The Air Force Development Test Center (AFDTC) recognized the growing requirements for using GPS in test programs and the need for a low cost, portable TSPI processing capability which sparked the development of the Desktop GPS Analyst. The Desktop GPS Analyst is a personal computer (PC) based software application for the generation of GPS-based TSPI.

International Telemetering Conference Proceedings / October 28-31, 1996 / Town and Country Hotel and Convention Center, San Diego, California / The Common Airborne Processing System (CAPS) provides a general purpose data reduction capability for digitally recorded data on a PC. PCM or MIL-STD-1553 data can be imported from a variety of sources into the CAPS standard file format. Parameter dictionaries describing raw data structures and output product descriptions describing the desired outputs can be created and edited from within CAPS. All of this functionality is performed on an personal computer within the framework of the graphical user interface provided by Microsoft Windows. CAPS has become the standard for digitally recorded data reduction on a PC at Eglin AFB and many other sites worldwide. New features, such as real-time inputs and graphical outputs, are being added to CAPS to make it an even more productive data reduction tool.

International Telemetering Conference Proceedings / October 28-31, 1996 / Town and Country Hotel and Convention Center, San Diego, California / This paper will discuss the design and performance of card level telemetry receivers and combiners. This will include products that have been designed to operate in compact computer controlled environments such as VME chassis, VXI chassis and personal computers using ISA buses. The paper will discuss design considerations required to overcome limitation of this environment such as noise and space. The paper will also discuss the performance of a telemetry receiver and combiner in this environment. This will include performance test results such as bit error rate test, phase noise measurements and combiner improvement measurements. Finally, the paper will discuss typical applications of card level telemetry receivers and combiners.