With the projected increase in global population, unprecedented increases in crop production will be needed and legume crops are one of the primary means of achieving these increases. The legume-Rhizobium symbiosis is the single most important source of biologically fixed nitrogen in agricultural systems but, as a biological system it is complex, and very sensitive to environmental effects, such as available soil nitrogen, soil pH (both high and low), soil salinity and extremes of soil temperature. Each of these may affect the delicate signal exchange process that occurs during symbiosis establishment. To better understand the effect of environmental factors on signal exchange and nodulation, we conducted four experiments, under controlled-environment conditions, with pea (Pisum sativum) as the model legume. The first experiment studied the effects of available nitrogen, the second the effects of low soil pH, the third the effect of soil salinity and the fourth the effects of low soil temperature. In all experiments the plants were inoculated with Rhizobium leguminosarum bv. viceae cells previously treated with 10 x 10-3 M of hesperitin or naringenin, or not treated (control). In all experiments plants were destructively sampled at 10, 20, 30 and 40 days after inoculation (in two experiments samplings were also conducted at 15 DAI), and data on plant and nodule variables were collected. To allow for a better understanding of the effects of flavonoids on nodule development an image analysis technique was developed that permitted us to measure every individual nodule at each sampling. This provided a more precise picture of nodule development over time than would have been possible with previous methods. Our results indicated that addition of flavonoids had positive effects on nodulation, both in number and size of nodules produced, and that the positive effects were greatest at the most inhibitory levels of the environmental factors tested, and at earlier sampling

Fatty acid biosynthesis from (1-$ sp{14}$C) acetate was optimized in plastids isolated from primary root tips of 7-day-old germinating pea seeds. Fatty acid synthesis was maximum at 82.3 nmol/hr/mg protein in the presence of 200$ mu$M acetate, 0.5mM each of NADH, NADPH and CoA, 6mM each of ATP and MgCl$ sb2$, 1mM each of MnCl$ sb2$ and glycerol-3-phosphate (G3P), 15mM KHCO$ sb3$, and 0.1M Bis tris propane, pH 8.0 incubated at 35C. At the standard incubation temperature of 25C, fatty acid synthesis was linear for up to 6 hours with 80 to 120 $ mu$g/ml plastid protein. ATP and CoA were absolute requirements, whereas divalent cations, potassium bicarbonate and reduced nucelotides all improved activity by 2 to 10 fold. Mg$ sp{2+}$ and NADH were the preferred cation and nucleotide, respectively. G3P and dihydroxyacetone phosphate had little effect, and dithiothreitol and detergents generally inhibited incorporation of $ sp{14}$C-acetate into fatty acid. / Glycerolipid synthesis was obtained from $ sp{14}$C-acetate, (U-$ sp{14}$C) G3P and (U-$ sp{14}$C) glycerol at relative rates of 3.7:1.0:0.1, respectively. (Abstract shortened by UMI.)

The effect of utilization of pulse flours with coarse and fine particle size milled from green lentils, yellow peas, navy beans, and pinto beans in pita bread and pan bread was studied. Composites containing 25, 50, 75, and 100% pulse flours were studied for pita baking, and 10, 15, and 25% for pan bread baking. Addition of the pulse flours produced pitas with the pockets, but they were smaller in diameter and firmer in texture than the wheat control. Supplemented pan bread had lower specific loaf volume and firmer crumb with coarser grain. The recommended tolerance levels of pulse flour addition were 25% for pita bread and 10-15% for pan bread depending on the pulse flour and particle size. It appears that navy beans and pinto beans are more suitable for baking applications using composite flours than lentils and yellow peas, and coarse flours produced breads with improved quality compared to fine flours.

Šio darbo tikslas buvo išanalizuoti ir įvertinti žirnių ir vikių mišinio įtaką pieno sudėčiai X ūkyje. Tikslui pasiekti buvo iškelti šie uždaviniai: 1. Išanalizuoti žirnių ir vikių mišinio įtaką pieno kiekiui ir sudėčiai. 2. Palyginti pieno sudėties rodiklius ganykliniu ir tvartiniu laikotarpiais. 3. Apibendrinti tyrimų rezultatus, pateikti išvadas ir pasiūlymus. Išvados: 1. Karvių, šertų žirnių ir vikių mišinio grūdų koncentruotaisiais pašarais, vidutinis pieno riebumas 0,08 proc.ir baltymingumas 0,04 proc. buvo mažesnis nei karvių, kurios buvo šertos visaverčiais kombinuotais pašarais. 2. Karvės, šertos visaverčiais kombinuotais pašarais, davė 0,023 kg pieno daugiau, nei karvės, šertos žirnių ir vikių mišinio koncentruotaisiais pašarais. 3. Laktozės kiekis piene buvo didesnis vidutiniškai 0,01 proc karvių , šėrtų žirnių ir vikių mišinio koncentruotaisiais pašarais. Laktozės kiekis statistiškai patikimas ( p<0,05). 4. Vidutinis urėjos kiekis abiejų grupių karvių piene buvo vienodas (18,77 mg/proc.). 5. Ganykliniu laikotarpiu 0,3 proc.(p<0,05) riebesnio ir 0,25 proc.(p<0,05) baltymingesnio pieno primelžta iš visaverčiais kombinuotais pašarais šertų karvių, o tvartiniu laikotarpiu 0,29 proc.(p<0,05) riebesnio pieno davė žirnių ir vikių mišinio miltais šertos karvės. / Tasks of work: overlook basis and ration of fodder given to milch-cows. Clarify how preparation of concentrated fodder, portioning out, and norming to the cows is managed. Identify what is the impact of concentrated mixture of vetch and bean stems to the cow’s productivity of milk composition. Generalize results of research, give conclusions and suggestions. Work placement and method. Work accomplished in X farm, in district of Rokiškis, duration 2011.11.01 – 2012.09.30. During the work: science literature was used, as well as statistical analysis, methods of data filing and summation. From results of research was found out that cows which were fed with concentrates of peas’s and vetch mixtures, average of milk fat 0.08% and proteins 0.04% was lower then cows which were fed full combined forage. Cows which were fed with complete combined forage gave 10.6kg more milk then those which were fed with concentrates of peas and vetch mixtures. Quantity of lactose in milk was higher average 0.01% of those cows which were fed with peas and vetch mixtures concentrates. Average urea quantity were equal (18,77 mg/%) in both cows groups. During pasturable period cows which were fed complete combined forage were milking 0.3% fatter and with more proteins 0.25% milk. During the cow-house period fatter milk 0.29% were given from cows which were fed of peas and vetch mixtures concentrates. In research it was observed lack of cellular tissue and protein in milk. Suggestion - pay... [to full text]

Typescript / Bibliography: leaves [137]-[157] / xx, 136 leaves : ill ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Agronomy, Waite Agricultural Research Institute, 1987

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 5 uppsatser.

Thesis (M. App. Sc.)--University of Adelaide, Dept. of Animal Science, 1995? / Copies of the author's previously published articles inserted. Includes bibliographical references (leaves 142-159).

The objective of this study was to examine the effects of feeding extruded and non-extruded mixtures of wheat dried distillers grains with solubles with peas (WP) or canola meal (WC) on ruminal fermentation, total tract nutrient digestion and milk production in dairy cows. Eight dairy cows (712 ± 54 kg body weight; 90 ± 31 days-in-milk at the beginning of the experiment) were used in a replicated 4 × 4 Latin square design (28-d periods) with a 2 × 2 factorial arrangement of dietary treatments. Four cows in one Latin square were fitted with rumen cannulas for the measurement of ruminal fermentation characteristics. Treatment diets contained either WP or WC combinations fed in an extruded or non-extruded form (16% of DMI). Diets were isonitrogenous (17.1% crude protein; CP) and contained approximately 53% concentrate and 47% forage (DM basis). Dietary treatment had no significant effect on DMI (P > 0.10). Starch intake was higher for cows fed extruded diets compared to those fed non-extruded diets (P = 0.028) and was also higher for cows fed WP compared to those fed WC (P = 0.042). Cows fed extruded diets had higher apparent ruminal digestion of DM (P = 0.02) and a tendency (P = 0.05) for a higher OM apparently digested in the rumen compared to those fed non-extruded diets. Total tract digestibilities of organic matter (P < 0.01), CP (P < 0.01), ether extract (P < 0.01) and starch (P = 0.047) were higher for cows fed extruded diets compared to those fed non-extruded diets. Total tract digestibility of ether extract was lower (P = 0.011) but digestibility of starch was higher (P < 0.01) and CP digestibility tended to be higher (P = 0.08) for cows fed WP compared to those fed WC. Fecal N excretion was lower in cows fed extruded diets compared to those fed non-extruded diets (P < 0.01), but there was no difference in N retention, productive N, RDP or RUP between diets (P > 0.10). Ruminal pH was higher for cows fed non-extruded WC compared to those fed extruded WC, but there was no difference between WP diets (interaction; P = 0.047). Ruminal acetate displayed the opposite interaction where concentration was highest for cows fed extruded WC and lowest for those fed non-extruded WC but there was no difference between WP diets (interaction; P = 0.019). Ruminal ammonia-N concentration tended to be higher for cows fed WC compared to those fed WP (P = 0.06). Ruminal propionate concentration was higher for cows fed extruded diets compare to those fed non-extruded diets (P = 0.026). Ruminal isobutyrate concentration was higher for cows fed WC compared to those fed WP (P < 0.01). Ruminal butyrate (P < 0.01) and isovalerate (P < 0.01) concentrations were higher for cows fed extruded WC compared to those fed non-extruded WC, but concentrations decreased for cows fed extruded WP compared to those fed non-extruded WP. Plasma glucose concentration was higher for cows fed WC compared to those fed WP but concentration was highest for cows fed extruded WC but lowest for cows fed extruded WP (interaction; P < 0.01). Milk protein yield (P = 0.047) was higher and milk yield tended to be higher (P = 0.06) for cows fed WP compared to those fed WC diets. Milk protein content was not affected by diet; however, milk fat content (P = 0.04) and MUN (P = 0.011) were lower, whereas milk yield (P = 0.030), 3.5% fat corrected milk yield (P = 0.027), milk fat yield (P = 0.027), lactose content (P = 0.011) and lactose yield (P < 0.01) were higher in cows fed the extruded diets compared to those fed non-extruded diets. In summary, these results indicate that extrusion had positive effects on overall milk production and total tract nutrient digestion.

Submitted by Claudinei Pracidelli (cpracide@ipen.br) on 2015-08-07T14:21:56Z No. of bitstreams: 0 / Made available in DSpace on 2015-08-07T14:21:56Z (GMT). No. of bitstreams: 0 / Tese (Doutorado em Tecnologia Nuclear) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Submitted by Claudinei Pracidelli (cpracide@ipen.br) on 2015-08-07T14:21:56Z No. of bitstreams: 0 / Made available in DSpace on 2015-08-07T14:21:56Z (GMT). No. of bitstreams: 0 / Profenofós é um organofosforado empregado como inseticida e acaricida amplamente utilizado no Brasil para o controle de pragas de cebolas, milho, soja, café, tomate, algodão, feijão, batata e outros. A irradiação é um processo empregado em todo o mundo e recomendada por diversos órgãos de saúde para a conservação de alimentos. A radiação ionizante utiliza raios gama, raios X ou aceleradores de elétrons e tem sido aplicada para eliminar ou reduzir a ação de agentes patogênicos e contribuir para aumentar o tempo de estocagem de vários alimentos. Os objetivos desse trabalho foram: (a) avaliar a degradação de soluções aquosas de profenofós submetidas à radiação ionizante, identificar e quantificar a formação de novos produtos por GC-MS; (b) analisar o efeito de feixe de elétrons em ervilhas inoculadas com soluções aquosas de profenofós; (c) sintetizar Polímeros Molecularmente Impressos (MIP) e Sílica Impressa Molecularmente (MIS), posteriormente, caracterizar os adsorventes em fase sólida e verificar sua seletividade para profenofós. O tratamento com aceleradores de elétrons com dose 31,6 kGy promoveu a formação de um novo produto de degradação e redução de 93,40 % de profenofós em soluções aquosas. Em ervilhas inoculadas com 1 &mu;g de profenofós submetidas à radiação ionizante de 30,4 kGy promoveu uma redução na concentração de profenofós em 57,46 %. Além disso, foram realizadas sínteses de MIP e MIS para a extração em fase sólida de profenofós. Os MIS sintetizados por sol-gel mostraram-se eficazes para o reconhecimento molecular e extração seletiva de profenofós. / Tese (Doutorado em Tecnologia Nuclear) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP