Whole yellow pea flour (WPF) and fractionated yellow pea flour (FPF) are novel functional food ingredients that vary in nutritional composition. Consequently, the health benefits of WPF and FPF remain undefined. The purpose of this research was to identify the effects of WPF and FPF on risk factors and morbidities associated with cardiovascular disease, diabetes and obesity as well as the gastrointestinal microbiome. Using USDA recommended dosages of WPF and FPF, clinical endpoints and the colonic microbiome were investigated using a human clinical trial engaging a cross-over design and a diet and energy controlled paradigm. Humans were also utilized to investigate post-prandial glycemic responses and sensory characteristics of novel functional foods formulated with WPF. Finally, Golden Syrian hamsters were used to assess the impact of high doses of WPF and FPF on clinical endpoints and caecal microbial abundance. Results reveal that USDA recommended dosages of WPF and FPF in humans decreased (p<0.05) fasting insulin and estimates of insulin resistance compared to white wheat flour (WF). Android-to-gynoid fat ratios in women were lower (p=0.027) in the WPF group compared to the WF group. FPF decreased (p<0.05) post-prandial energy expenditure alongside a tendency (p<0.075) to reduce carbohydrate oxidation. Novel biscotti and banana bread formulated with WPF induced low post-prandial glycemic responses which were similar to boiled whole yellow peas and significantly lower (p<0.05) than white bread. Sensory analysis of novel WPF biscotti and banana bread demonstrated that WPF-based food products are palatable and acceptable for human consumption. Hamsters consuming diets containing 10% WPF and FPF induced similar reductions (p<0.05) in fasting insulin levels compared to controls. However, animals consuming WPF increased (p<0.05) oxygen consumption while FPF decreased (p<0.05) fasting glucose levels. In addition, terminal restriction fragment length polymorphism analysis revealed that WPF and FPF induced distinct shifts in caecal microbial populations within the phyla Firmicutes. Finally, pyrosequencing analysis of human fecal microbiota demonstrated that FPF and WPF induced shifts in bacterial genera, primarily within Bacteroidetes and Firmicutes. In conclusion, whole and fractionated yellow pea flours are functional food ingredients and can be utilized to manage risk factors for lifestyle-related diseases in humans.

Typescript / Includes bibliographical references / 134 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1966

The purpose of this study was to explore the influence of external product attributes on consumers’ perceived acceptance of pea protein as a protein substitute in food products. The interest in pea protein as a possible protein source was investigated and the various advantages of consuming pea protein were highlighted. In previous studies, pea protein was identified as a possible substitute for red meat (Davies & Lightowler, 1998), but also as protein containing the amino acid profile which corresponds with the profile recommended for adults by the Food and Agriculture Organisation (FAO) (Swendseid, 1981). Additionally the nutritional and health benefits, the environmental benefits of growing pea protein as well as the non-allergen declaration accompanying pea protein were discussed. The various factors that will influence the consumer’s decision making process when introduced to a new and unfamiliar protein source such as pea protein were investigated in terms of the external product attributes as well as the factors indicated in the Theory of Reasoned Action (TRA) (Fishbein & Ajzen, 1975). The external product attributes were identified as the influence of price, marketing of the product, packaging attributes of new food products, branding importance of a product and new product education and knowledge perceived necessary for acceptability of a new food product. The additional factors which were investigated, as indicated by the TRA, included the influence of the subjective norms or significant others, as well as the consumers’ personal attitude. attitude. Due to the limited insight and understanding of the influences that external product attributes have on a consumer’s perceived acceptance of pea protein as a protein substitute in food products, a qualitative exploratory research design was initiated. An exploratory research design was incorporated because this afforded the researcher the opportunity to gather thorough and comprehensive data or information and it also increased the richness and depth of explorations that could be researched (Key, 2002). The fact that an exploratory research design was incorporated, a non-probability sampling strategy was implemented. Additionally a convenient sampling strategy was also implemented because this type of sampling strategy represents sites or individuals from which data can easily be collected and where access is convenient (Cresswell, 2007). The unit of analysis for this research was female shoppers, being the main grocery and food shoppers in the household, who were living in suburban areas. The participants of the research, therefore, resided mainly in the Johannesburg and Pretoria suburban areas, within the Gauteng province and were chosen because these are two of the major suburban cities in South Africa. The data were collected through means of a multi-method approach whereby different data collection instruments were applied. In this regard focus group interviews, sentence completion exercise and a vignette were used which addressed the objectives set out in the study. From the findings of the study on the influence of external product attributes on consumers’ perceived acceptance of new products in general and new products containing pea protein, it was found that there were not any noticeable differences between a new food product and a new food product containing pea protein. The consumer did not indicate a greater willingness to accept general new products in comparison to a pea protein product. On the contrary, the fact that a new product contained pea protein did not make any difference. For consumers external product attributes in general played a major role in their purchasing decision making process. The most important requirement that the consumers had was that additional information regarding the pea protein should be provided. It was indicated by the participants that they would be interested in the pea protein product and consider purchasing it and incorporating it into their daily diet, providing they knew more about the product and had sufficient background information about the new product. Furthermore, the importance of marketing of a new product was highlighted and the fact that the packaging of the new product should be attractive and eye-catching. Price competiveness was mentioned, however, should a new and unfamiliar product be introduced by a well-known brand, this would lower the initial resistance to the new product. One of the main limitations of this study is the relatively small sample size from a particular area in South Africa and, therefore, resulting in a very selective sample. The sample, therefore, remains one of convenience which limits the ability to generalize or transfer findings to a bigger group of consumers. Furthermore the study location was within a limited area, namely Gauteng. For this reason no assumptions were made nor were findings considered to be transferable to other population groups or consumer groups. Another limitation is that the topic of this study is very new and unfamiliar; there are, therefore, no previous studies with which to compare the findings. This study was exploratory in nature and the instruments were chosen specifically to explore a new and unfamiliar topic. The transferability of this study might be limited, but care was taken to describe the research context in as much detail as possible. In doing so, an attempt was made to enhance the transferability, should another researcher wish to duplicate the study. From the findings of this study certain recommendation can be made as most participants were of the opinion that they would be interested in the pea protein product and consider purchasing it and incorporating it into their daily diet provided they knew more about the product and sufficient background information had been supplied. Furthermore, it was noted from the findings that these external attributes would influence the new product acceptability and, therefore, giving the research and developers as well as the marketing team of a manufacturing company the information necessary to understand the requirements from the consumers regarding the product containing pea protein better and how this product should be marketed as a healthy alternative to other vegetable protein sources. Further recommendations are that should a food manufacturing company wish to develop a pea protein product, they would need to promote the product beforehand and create an awareness of the advantages and benefits of pea proteins. This can be done by editorials in magazines as well as promotions in printed media. Additionally, the competition between products and brands is fierce and, therefore, manufacturing companies need to differentiate their products to keep consumers interested in their product or brand. Pea protein is a new and innovative product and can, therefore, add that differentiation and benefit to food products. Future research that can be considered is that of a sensory study, to determine the actual viability of the pea protein products based on sensory evaluation. One of the main criteria indicated for the acceptability of a food product containing pea protein has been the taste. The present research was theoretical and exploratory in nature and, therefore, there were no actual products which the participants could taste. Additionally, the inclusion criteria for this study was identified as female shoppers who are in charge of household grocery and food purchases and were, therefore, specifically recruited to participate in the research. However, based on a study done by Jensen (2011), it is recommended that further studies specifically include males because men are doing more of the household grocery shopping and they are not as brand loyal as females and would, therefore, be more willing to try and experiment with new food products. A study based on the gender differences of the acceptability of new products, could highlight different extrinsic attributes that would influence the purchasing decision. / Business Management / M. Consumer Sc.

The nutritional value of faba beans (Vicia faba), Australian sweet lupins (Lupinus angustifolius), white lupins (Lupinus albus) and peas (Pisum sativum) grown in New Zealand for broilers were evaluated in terms of their nutritional characteristics, protein quality (protein efficiency ratio), apparent metabolisable energy, apparent ileal digestibility coefficient of amino acids and the effects on bird performance. The effects of dehulling and extrusion cooking on the nutritive value of legumes were also investigated. The first experiment discussed in Chapter 3 evaluated the effect of cultivars on the nutrient profile and protein quality of chickpeas (Cicer arietinum), Australian sweet lupins, peas and soybeans (Glycine max). With the exception of white lupins, cultivars had no effect on the proximate and fibre composition of grain legumes. Starch was the primary carbohydrate component of chickpeas and peas, whilst non-starch polysaccharides were the major carbohydrates in lupins. The legume proteins were deficient in lysine, methionine, cystine and threonine. No differences were found in protein quality between cultivars of the different grain legume species. The lowest weight gain and protein efficiency ratio, in addition to the highest relative pancreatic weight and mortality rate was found in raw soybeans, suggesting that soybeans contained high a concentration of anti-nutritional factors, such as protease inhibitors. Birds fed chickpeas, lupins and peas had a low mortality rate and relative pancreatic weight, confirming that the level of anti-nutrients in these legume seeds was low. The apparent metabolisable energy and apparent ileal digestibility coefficient of amino acids of faba beans, Australian sweet lupins, white lupins and peas were determined in the second experiment (Chapter 4). Cultivar effect on the apparent metabolisable energy values was observed only for faba beans and white lupins. Faba beans, white lupins and peas had comparable apparent metabolisable energy values, but these values were higher than those of Australian sweet lupins, and lower than that of soybean meal. No cultivar differences were found in the apparent ileal digestibility coefficient of amino acids of grain legumes. The apparent ileal digestibility coefficient of amino acids of both lupin species was found to be comparable to that of soybean meal. The effects of feeding diets containing 200 g/kg faba beans, lupins or peas on the performance, digestive tract development and litter quality of broilers were investigated in the third and fourth trials. In the cage trial (Chapter 5), the results showed that the weight gain of birds fed diets containing grain legumes was similar to that of control diet. Feed intake and feed per gain of birds fed diets containing the majority of grain legume cultivars did not differ from those fed the maize-soy diet. Birds fed diets containing faba beans had more dry and friable excreta compared to other treatment diets. The performance of birds fed diets containing 200 g/kg grain legumes during the 35 d grow-out period, in the floor pen trial (Chapter 6), confirmed the results of the cage trial. In this trial, weight gain and feed per gain of birds fed diets without meat meal were superior to those with meat meal. In cage trials, the modification of some segments of digestive tract development was probably due to the dietary NSP. Whilst in floor pen trial, digestive tract development was not influenced by the inclusion of grain legumes. The effect of methodology of determination (direct vs. difference method) on the apparent ileal digestibility coefficient of amino acids of wheat, maize, Australian sweet lupins, peas and soybean meal for broilers was evaluated in the fifth study (Chapter 7). The influence of methodology on apparent ileal digestibility coefficient of amino acids was found to vary amongst the feed ingredients. In general, the apparent ileal digestibility coefficient of amino acids of test ingredients determined by the difference method was higher than those determined by the direct method, suggesting that the use of the direct method may underestimate the apparent ileal digestibility coefficient of amino acids in low and medium protein ingredients. Data reported in Chapter 8 shows that dehulling increased the apparent metabolisable energy values of faba beans and Australian sweet lupins, but it had no beneficial effect on peas. The increase of apparent metabolisable energy values may be attributed to the decrease in non-starch polysaccharides of these legume seeds after dehulling. The removal of hulls increased the amino acid concentrations, but it had no effect on the apparent ileal digestibility coefficient of most amino acids. These results suggest that dehulling of grain legumes would be nutritionally beneficial and, likely to be economical in view of the improved amino acid concentrations and energy values. The final experiment (Chapter 9) demonstrated that extrusion of peas markedly influenced the content of crude protein, non-starch polysaccharides, starch, and trypsin inhibitors. The soluble non-starch polysaccharides and trypsin inhibitor contents of the majority of extruded pea samples were higher than those of raw peas, but insoluble and total non-starch polysaccharides decreased with extrusion. Extrusion had no effect on the apparent ileal protein digestibility and the apparent metabolisable energy of peas, but it increased ileal starch digestibility.

This work is inserted in the group Saberes e Práticas Educativas (Knowledge and Education Practices) Mastership Course in the Education Post Graduation Program of Federal University in Uberlândia, Minas Gerais. This research aims to identify high school students sexuality representations in a public school, located in Uberlândia, Minas Gerais, involved in the Education Program for Youth Care (Programa Educacional de Atenção ao Jovem PEAS Juventude). We seek to further characterize, if any, the differences between the representations of the students which were members of the Program and the other high school students in the institution. The research took place in the period between May and December, 2010 and took as inspiration the methodological approach of qualitative case study. Information production took place from the application of a questionnaire to 118 students those, 43 were treated by PEAS Juventude and 75 weren t. We made also direct observations of the Program workshops and classes from three classes of high school. Based on the information obtained, we can consider that the representations of students PEAS Juventude members and nom members are similar when they discourse on sexuality. In relation to activities under the Program, we would like to emphasize that the thematic affectivity and sexuality, youth and citizenship, the workplace and life expectancy were not considered during the proposed activities with multiplication purposes. The teen-agers build and share representations of \"staying\" and \"dating\" that are marked by the criterion of faithfulness and by the differences between a stable relationship of a transient. According to them the \"staying\" relationship is casual, commitment-free, while \"dating\" relationship is serious, enduring and faithful. Pregnancy was represented as a problem by both groups of students surveyed. For them, pregnancy during the teenage youth implies responsibilities and hardships which were considered by them as impossibilities to youth experience that they could have concerning to violence, adolescents share the representation that infidelity justifies physical assault. The students PEAS Juventude members recognize that violence against women is a coward and criminal act, although some of them have said that before the attack is justified retribution. Participants and not-program participants students shared representations biased and fraught with discrimination on homosexuality. Although this thesis does not exhaust the possibilities of further research on this subject, we hope to contribute to discussions about youth sexuality. / Este trabalho está inserido na Linha de Saberes e Práticas Educativas do curso de Mestrado do Programa de Pós-Graduação em Educação da Universidade Federal de Uberlândia. A presente pesquisa teve como objetivo identificar as representações de sexualidade dos/as estudantes de Ensino Médio de uma escola pública da rede estadual de ensino, localizada em Uberlândia, Minas Gerais, envolvidos/as com o Programa Educacional de Atenção ao Jovem PEAS Juventude. Buscamos, ainda, caracterizar, se existentes, as diferenças entre as representações dos/as discentes integrantes do Programa e dos/as demais alunos/as que cursavam o Ensino Médio na escola. A pesquisa aconteceu no período compreendido entre maio e dezembro do ano de 2010 e tomou como inspiração metodológica a abordagem qualitativa do tipo estudo de caso. A produção de informações se deu a partir da aplicação de questionário a 118 alunos/as, sendo 43 discentes integrantes do PEAS Juventude e 75 não integrantes do Programa e de observações diretas das oficinas do Programa e das aulas de três turmas do Ensino Médio. Com base nas informações obtidas, podemos considerar que as representações de discentes integrantes e não integrantes do PEAS Juventude se aproximam quando discursam sobre sexualidade. Esses/as adolescentes compartilham as mesmas opiniões ao afirmarem que a família representa a instituição social que mais oferece segurança, confiabilidade e conforto no momento de sanar dúvidas e levantar informações sobre sexualidade. Em relação às atividades previstas pelo Programa, gostaríamos de enfatizar que os eixos temáticos afetividade e sexualidade, juventude e cidadania, mundo do trabalho e perspectiva de vida não foram contemplados durante a atividade proposta com a finalidade de multiplicação. Os/as adolescentes constroem e compartilham representações sobre ficar e namorar que são marcadas pelo critério da fidelidade e das diferenças entre um relacionamento estável de um transitório. Segundo eles/as, ficar representa o relacionamento casual, isento de compromisso, enquanto namorar representa relacionamento sério, duradouro e fiel. A gravidez recebeu a representação de problemática, comum aos dois grupos de discentes pesquisados. Para os/as adolescentes, uma gestação durante a juventude implicaria responsabilidades e privações que foram consideradas por eles/as como impossibilidades de vivenciarem oportunidades que a juventude poderia proporcionar-lhes. Quanto à violência, os/as adolescentes partilham da representação de que a infidelidade justifica as agressões físicas. Os/as alunos/as integrantes do PEAS Juventude reconhecem que a violência contra a mulher é covarde e criminosa, embora alguns tenham afirmado que, diante de uma agressão, o revide é justificado. Alunos/as participantes e não participantes do Programa partilharam representações preconceituosas e carregadas de discriminações acerca da homossexualidade. Ainda que a presente dissertação não esgote a possibilidade de futuras pesquisas sobre esta temática, espera-se que contribua para as discussões em torno da sexualidade juvenil. / Mestre em Educação

Submitted by Renata Lopes (renatasil82@gmail.com) on 2017-03-22T11:56:40Z No. of bitstreams: 1 roneypolatodecastro.pdf: 1485038 bytes, checksum: cc79d238b696ec48a1a15996ef770c75 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-03-22T12:43:21Z (GMT) No. of bitstreams: 1 roneypolatodecastro.pdf: 1485038 bytes, checksum: cc79d238b696ec48a1a15996ef770c75 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-03-22T12:44:48Z (GMT) No. of bitstreams: 1 roneypolatodecastro.pdf: 1485038 bytes, checksum: cc79d238b696ec48a1a15996ef770c75 (MD5) / Made available in DSpace on 2017-03-22T12:44:48Z (GMT). No. of bitstreams: 1 roneypolatodecastro.pdf: 1485038 bytes, checksum: cc79d238b696ec48a1a15996ef770c75 (MD5) Previous issue date: 2008-04-01 / Apropriando-se das características de “movimento”, “imprevisibilidade” e “inconstância” das viagens, a pesquisa teve como foco os “sentidos” e as “possibilidades” que a “presença” do Programa de Educação Afetivo-Sexual (PEAS) mobilizou em seus participantes que atuam em três escolas municipais de Juiz de Fora (MG). A dissertação apresenta as análises empreendidas a partir de alguns temas específicos: sexualidade, educação sexual, formação de identidades, produção de sujeitos, relações de poder na escola e formação docente. A referência teórico-metodológica que embasa as análises está ancorada na perspectiva pósestruturalista e nas teorizações do filósofo Michel Foucault, permitindo o fomento e a multiplicação das questões, sem o objetivo de apresentar respostas ou conclusões definitivas. As principais questões da pesquisa, que deram margem a diversas outras, foram: que sentidos são atribuídos ao PEAS por professoras e outros profissionais(as) que participam de sua “capacitação”? Que tipos de sujeitos são produzidos e o que estes sujeitos produzem nos contextos de ações do PEAS? Que possibilidades esses sujeitos vêem para o PEAS e para a educação sexual na escola? As problematizações apontam para as formas diversas pelas quais o PEAS se associa ao cotidiano da escola, incorporando-se às relações de poder presentes nesse espaço. Isso nos permite argumentar que o programa atua como tecnologia de produção de sujeitos e identidades, disponibilizando determinadas formas de compreender e vivenciar as relações afetivo-sexuais. / Appropriating of the characteristics of “movement”, “unpredictability” and “inconstancy” of travellings, the research has focused on the “senses” and the “possibilities” that the “presence” of the Love-Sexual Education Program (LSEP) has mobilized in its participants that act in three county public schools in Juiz de Fora (MG). The work presents the analysis undertaken from some specific themes: sexuality, sexual education, identity formation, subject production, power relationship in the school and teaching formation. The theoreticalmethodological reference that builds a foundation for the analysis is anchored in the postestructured perspective and in the theory by the philosopher Michel Foucault, allowing the development and the multiplication of questions, without the objective of presenting answers or definitive conclusions. The main questions of the research, that gave occasion to several others, were: what senses are attributed to LSEP by teachers ant other professional(s) that participate of its “capacitation”? Which types of subjects are produced and what do these subjects produce in the contexts of LSEP actions? Which possibilities do these subjects see for LSEP and for sexual education in school? The problems point to the different forms to which LSEP associates itself to school quotidian, incorporating itself to power relationships present in these space. This allows is to argue that the program acts as technology of subject and identity productions, turning available determined forms of comprehending and living the love-sexual relationships.

Profenofós é um organofosforado empregado como inseticida e acaricida amplamente utilizado no Brasil para o controle de pragas de cebolas, milho, soja, café, tomate, algodão, feijão, batata e outros. A irradiação é um processo empregado em todo o mundo e recomendada por diversos órgãos de saúde para a conservação de alimentos. A radiação ionizante utiliza raios gama, raios X ou aceleradores de elétrons e tem sido aplicada para eliminar ou reduzir a ação de agentes patogênicos e contribuir para aumentar o tempo de estocagem de vários alimentos. Os objetivos desse trabalho foram: (a) avaliar a degradação de soluções aquosas de profenofós submetidas à radiação ionizante, identificar e quantificar a formação de novos produtos por GC-MS; (b) analisar o efeito de feixe de elétrons em ervilhas inoculadas com soluções aquosas de profenofós; (c) sintetizar Polímeros Molecularmente Impressos (MIP) e Sílica Impressa Molecularmente (MIS), posteriormente, caracterizar os adsorventes em fase sólida e verificar sua seletividade para profenofós. O tratamento com aceleradores de elétrons com dose 31,6 kGy promoveu a formação de um novo produto de degradação e redução de 93,40 % de profenofós em soluções aquosas. Em ervilhas inoculadas com 1 &mu;g de profenofós submetidas à radiação ionizante de 30,4 kGy promoveu uma redução na concentração de profenofós em 57,46 %. Além disso, foram realizadas sínteses de MIP e MIS para a extração em fase sólida de profenofós. Os MIS sintetizados por sol-gel mostraram-se eficazes para o reconhecimento molecular e extração seletiva de profenofós. / Profenofos is an organophosphate widely used in Brazil as insecticide and acaricide in the control of pests in onions, corn, soybeans, coffee, tomato, cotton, beans, potatoes among others. Irradiation is a process used worldwide and recommended by many health agencies for food preservation. Food irradiation preserving process uses accelerated electrons, gamma rays or X-rays. Ionizing radiation treatment is applied to eliminate or to reduce the action of pathogens and to increase the shelf life of some foods. The objective of this study were (a) to evaluate the degradation of aqueous solutions of Profenofos by ionizing radiation, identify and quantify the formation of new products by GC-MS; (b) to analyze the effects of electron beam in peas inoculated with aqueous solutions of Profenofos; (c) to synthesize Molecularly Imprinted Polymer (MIP) and Molecularly Imprinted Silica (MIS), subsequently characterize the adsorbents in solid phase and check its selectivity for profenofos. The treatment with electron accelerators with 31.6 kGy dose promoted the formation of a new by-product and 93.40 % reduction of profenofos in aqueous solutions. In peas inoculated with 1 &mu;g of profenofos by ionizing radiation of 30.4 kGy promoted a reduction of 57.46 % in the concentration of profenofos. Furthermore, the MIP and MIS were performed for solid phase extraction of profenofos. The MIS synthesized by sol-gel proved to be effective for the recognition molecular and selective extraction of profenofos.

Profenofós é um organofosforado empregado como inseticida e acaricida amplamente utilizado no Brasil para o controle de pragas de cebolas, milho, soja, café, tomate, algodão, feijão, batata e outros. A irradiação é um processo empregado em todo o mundo e recomendada por diversos órgãos de saúde para a conservação de alimentos. A radiação ionizante utiliza raios gama, raios X ou aceleradores de elétrons e tem sido aplicada para eliminar ou reduzir a ação de agentes patogênicos e contribuir para aumentar o tempo de estocagem de vários alimentos. Os objetivos desse trabalho foram: (a) avaliar a degradação de soluções aquosas de profenofós submetidas à radiação ionizante, identificar e quantificar a formação de novos produtos por GC-MS; (b) analisar o efeito de feixe de elétrons em ervilhas inoculadas com soluções aquosas de profenofós; (c) sintetizar Polímeros Molecularmente Impressos (MIP) e Sílica Impressa Molecularmente (MIS), posteriormente, caracterizar os adsorventes em fase sólida e verificar sua seletividade para profenofós. O tratamento com aceleradores de elétrons com dose 31,6 kGy promoveu a formação de um novo produto de degradação e redução de 93,40 % de profenofós em soluções aquosas. Em ervilhas inoculadas com 1 &mu;g de profenofós submetidas à radiação ionizante de 30,4 kGy promoveu uma redução na concentração de profenofós em 57,46 %. Além disso, foram realizadas sínteses de MIP e MIS para a extração em fase sólida de profenofós. Os MIS sintetizados por sol-gel mostraram-se eficazes para o reconhecimento molecular e extração seletiva de profenofós. / Profenofos is an organophosphate widely used in Brazil as insecticide and acaricide in the control of pests in onions, corn, soybeans, coffee, tomato, cotton, beans, potatoes among others. Irradiation is a process used worldwide and recommended by many health agencies for food preservation. Food irradiation preserving process uses accelerated electrons, gamma rays or X-rays. Ionizing radiation treatment is applied to eliminate or to reduce the action of pathogens and to increase the shelf life of some foods. The objective of this study were (a) to evaluate the degradation of aqueous solutions of Profenofos by ionizing radiation, identify and quantify the formation of new products by GC-MS; (b) to analyze the effects of electron beam in peas inoculated with aqueous solutions of Profenofos; (c) to synthesize Molecularly Imprinted Polymer (MIP) and Molecularly Imprinted Silica (MIS), subsequently characterize the adsorbents in solid phase and check its selectivity for profenofos. The treatment with electron accelerators with 31.6 kGy dose promoted the formation of a new by-product and 93.40 % reduction of profenofos in aqueous solutions. In peas inoculated with 1 &mu;g of profenofos by ionizing radiation of 30.4 kGy promoted a reduction of 57.46 % in the concentration of profenofos. Furthermore, the MIP and MIS were performed for solid phase extraction of profenofos. The MIS synthesized by sol-gel proved to be effective for the recognition molecular and selective extraction of profenofos.

[Truncated abstract] Soils of Western Australian cropping regions are very low in phosphorous. White lupin, chickpea, and faba bean are being increasingly used in rotations with wheat on these soils. Yield of wheat after a legume crop is frequently higher than its yield after wheat. It has been reported that in addition to nitrogen, legumes can also contribute to improve the availability of phosphorous for the subsequent crops. This PhD research project aimed at optimising the economic returns of wheat-legume rotations through more efficient use of P fertiliser in the legume phase as well as enhanced availability of soil P in the subsequent wheat phase

The diploma thesis deals with the development of the market for protein crops. The literature research deals one hand with the distribution and morphology of legumes, and on second hand with the structure and chemical composition of seeds of selected species (pea, soybean and lupine). The literature research also covers the production of protein concentrates and their commercialization. The practical part is mainly engaged in the analysis of market developments of selected species of legumes in the Czech Republic, especially during the years 2000 - 2015, in comparison with the market development of the individual European states (Germany, Austria, Poland, Slovakia and France). The outcome of this diploma thesis is the ordination of statistical data available in clear graphical and tabular outputs, it does not miss time series evaluation of development of individual crops in both the Czech Republic and other selected countries.