The final thesis is dealing with long-term preservation of document and related issues with it. At the beginning of the thesis are defined terms such as paper and digital document and lifecycle it has to go through. Next parts are devoted to selected standards and recommendations, metadata, the most widely used approaches in this area, which are migration and emulation, most used technologies and the last part of this section is dedicated to security meters. The survey deals with still active projects which start at the beginning of last year. These projects show new trends and possibilities in this area. From twelve project were selected four interesting of them (ARCOMEM, ENSURE, TIMBUS, SCAPE) which are subsequently described from different perspectives, compared and evaluated.

The aim of this work is a comprehensive grasp of significant factors intervening into the operation and structure of heritage preservation in the Federal Republic of Germany in connection with the Swiss Confederation. The work focuses on the issue of financial support for cultural heritage according to the type of property sites, especially in public administration. Attention is also paid to the perspectives of continuity and long-term effectiveness of projects in terms of available social tools. After reading this thesis the reader will be able to orient themselves in the system of heritage preservation and to determine the effectiveness of funds spent on culture in European countries.

For rare book collections, a considerable challenge is involved in evaluating collection materials in terms of their inherent value, which includes the textual and intangible information the materials provide for the collection's users. Preservation management in rare book collections is a complex and costly process. As digitization and other technological advances in surrogate technology have provided new forms representation, new dilemmas in weighing the rare book's inherently valuable characteristics against the possibly lesser financial costs of surrogates have arisen. No model has been in wide use to guide preservation management decisions. An initial iteration of such a model is developed, based on a Delphi-like iterative questioning of a group of experts in the field of rare books. The results are used to synthesize a preservation management framework for rare book collections, and a small-scale test of the framework has been completed through two independent analyses of five rare books in a functioning collection. Utilizing a standardized template for making preservation decisions offers a variety of benefits. Preservation decisions may include prioritizing action upon the authentic objects, or developing and maintaining surrogates in lieu of retaining costly original collection materials. The framework constructed in this study provides a method for reducing the subjectivity of preservation decision-making and facilitating the development of a standard of practice for preservation management within rare book collections.

Whole grain and bran samples of two Malawian sorghums, Phatafuli, (a brown-coloured condensed tannin variety) and Shabalala, (a white-coloured condensed tannin-free variety) were analysed for their content of total phenols, condensed tannins and antioxidant activities. The effect of oxidizing conditions during extraction, and the storage stability of a freeze-dried crude phenolic extract (CPE) from the condensed tannin sorghum bran as influenced by packaging, storage temperature and length of storage, in relation to its content of total phenols, condensed tannins and antioxidant activity was also investigated. Antioxidant activity of the CPE, in comparison with tertiary butyl hydroquinone (TBHQ), was then evaluated in sunflower oil at concentrations of 1000, 1500 and 2000 ppm in the absence or presence of ferric ions at 2.2 and 4.4 ppm in the dark at 65oC.Progression of oxidation was monitored by measurement of peroxide values (PV) and anisidine values (AV) during a 14-day storage period. Phatafuli contained higher content of total phenols and antioxidant activity than Shabalala both in the whole grain and the bran, probably due to the presence of condensed tannins in Phatafuli sorghum, which were not detected in Shabalala sorghum. For both sorghum varieties, the bran contained higher levels of total phenols and antioxidant activity than the whole grain, confirming that phenolic compounds in sorghum are largely concentrated in the bran. Antioxidant activities of the sorghum varieties correlated highly with their total phenol and condensed tannin contents, suggesting that the phenolic compounds were largely responsible for the antioxidant activities of the sorghum grains. Bubbling of oxygen into the liquid crude phenolic extract did not have any significant effect on the parameters tested. Similarly, vacuum-packed samples did not differ significantly in the parameters tested from the samples that were not vacuum-packed. CPE samples stored at –20oC had significantly higher levels of total phenols, condensed tannins and antioxidant activity than those stored at 25oC during some days of storage. Storage time was however the major factor influencing the levels of total phenols, condensed tannins and antioxidant activity of the CPE from Phatafuli sorghum during storage, which suggested that CPE from condensed tannin sorghum bran might need to be used shortly after extraction to ensure optimum antioxidant activity. There was an insignificant correlation between the antioxidant activities of the CPE and their phenolic contents during storage, which could have been due to the formation of new compounds with a lower antioxidant capacity. The CPE inhibited oxidation of sunflower oil as shown by lower peroxide values and anisidine values compared to control samples. The CPE was however less effective in reducing peroxide values compared to TBHQ, but was similar to TBHQ in reducing anisidine values. In the presence of ferric ions, the CPE appeared to be less effective in reducing peroxide values compared to TBHQ, but appeared to be more effective than TBHQ in reducing anisidine values. The results showed that the tannin sorghum bran CPE appeared to act as both lipid radical scavengers and metal chelators. The CPE however imparted colour to the sunflower oil, which could limit its application as a natural antioxidant in edible oils. / Dissertation (MSc(Agric) Food Science and Technology)--University of Pretoria, 2008. / Food Science / unrestricted

O uso de protocolos de diluição bitérmica está aumentando em centros de inseminação artificial e é necessário garantir que as doses de inseminação tenham a mesma qualidade quando comparado com protocolos de diluição isotérmica. Quatro ejaculados de cada 19 reprodutores PIC® foram coletados e distribuídos em split sample em três tratamentos: diluição bitérmica em duas etapas (T1), diluição isotérmica em duas etapas (T2) e diluição isotérmica em uma etapa (T3). A curva de temperatura para os três tratamentos foi feita utilizando um data logger com sensor de temperatura. As doses inseminantes foram preparadas utilizando o diluente BTS e armazenadas a 16ºC e usadas para avaliação dos parâmetros espermáticos através do Sistema CASA e avaliação de morfologia espermática, durante 120 horas. A temperatura das amostras de sêmen submetidas à diluição bitérmica em duas etapas alcançou 24,1ºC durante 120 minutos , enquanto que as amostras submetidas à diluição isotérmica em uma ou duas etapas alcançou 26,8ºC e 27,0ºC, respectivamente. A motilidade total, a progressiva e BCF foram influenciadas (P<0,05) pelo tempo de diluição, mas não pelo protocolo de diluição. A motilidade total e progressiva diminuiu com o tempo de armazenamento (91.0 ± 0.91 para 81.5 ± 1.08 % e 74.0 ± 2.48 para 60.4 ± 2.59% de 24h para 120h, para MOT e PROG, respectivamente) enquanto BCF diferiu entre 24 e 120h (28.6 ± 0.76 e 27.3 ± 0.79 Hz). As seguintes características de motilidade não foram afetadas pelo protocolo de diluição e pelo tempo de armazenamento: DAP, DCL, DSL, VAP, VCL, VSL, STR, LIN, WOB e ALH. Às 72 h de armazenamento, a morfologia espermática não diferiu entre os tratamentos (P>0.05), apresentando uma média geral de 9.2 ± 0.36 defeitos totais. Concluindo, a diluição bitérmica torna o processo de produção de doses inseminantes mais rápido, pois demoraram menos tempo para alcançar a temperatura próxima de armazenamento, sem comprometer a qualidade das doses inseminantes produzidas. / The use of bithermic dilution protocols is increasing in artificial insemination centers, being necessary to guarantee that the quality of insemination doses remain the same when compared to isothermic dilution protocols. Four ejaculates from each one of 19 crossbreed PIC® boars were collected and assigned, in a split sample design, in to three treatments: two step bithermic dilution (T1), two step isothermic dilution (T2) and one step isothermic dilution (T3). Temperature curve for the three treatments was recorded using a temperature sensor data logger. Semen doses prepared with BTS extender were stored at 16°C and were used to evaluate sperm parameters through CASA system and sperm morphology, during 120 h. The temperature in semen samples submitted to a two-step bithermic dilution reached 24.1ºC during 120 min, whereas one or two-step isothermic dilution samples reached 26.8ºC and 27.0ºC, respectively. Total motility, progressive motility and BCF were influenced (P<0.05) by the storage time but not by the dilution procedure. Total and progressive motility reduced throughout the storage time (91.0 ± 0.91 to 81.5 ± 1.08 % and 74.0 ± 2.48 to 60.4 ± 2.59% from 24h to 120h, for MOT and PROG respectively) whereas BCF differed between 24 and 120h (28.6 ± 0.76 and 27.3 ± 0.79 Hz). The following motility traits were neither affected by the dilution procedure nor by the time of storage: DAP, DCL, DSL, VAP, VCL, VSL, STR, LIN, WOB, and ALH. At 72 h of storage, sperm morphology was not different among treatments (P>0.05), showing an overall mean of 9.2 ± 0.36 total defects. In conclusion, the bithermic dilution makes the process of artificial insemination doses production faster by taking less time to reach a temperature close to that of storage, without impairing semen quality.

Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-07-26T12:57:02Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) CRUZ, C K M.pdf: 6032813 bytes, checksum: 7efac88640dc87672373f58d5ea10713 (MD5) / Made available in DSpace on 2017-07-26T12:57:02Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) CRUZ, C K M.pdf: 6032813 bytes, checksum: 7efac88640dc87672373f58d5ea10713 (MD5) Previous issue date: 2016-01-29 / CNPQ / O presente trabalho teve por objetivo analisar os fatores de influência nos processos de tombamento e suas consequências na preservação do patrimônio material e arqueológico em quatro parques históricos da Região Metropolitana do Recife. A questão da busca por uma memória nacional vivida nas décadas de 1960 a 1980 aqui no Brasil exerceu uma forte influência nas escolhas de que bens patrimoniais constituíam exemplos da identidade formadora da União. Utilizando os processos de tombamento como norteadores, analisamos as estratégias de tombamento realizadas em Pernambuco, avaliamos o discurso político, ideológico que influenciou esses processos de tombamento e, por fim, analisamos as estratégias de tombamento e preservação do patrimônio arqueológico dos parques históricos e/ou arqueológicos do país onde o fator arqueológico foi o principal motivo para a realização do processo de tombamento. Para isso, traçamos um protocolo para análise das dimensões técnica, jurídica e social. E, a partir disso elencamos cinco elementos principais que nos permitiram relacionar os discursos e as ações preservacionistas a que foram submetidos esses patrimônios. Assim, obtivemos que os parques históricos em Pernambuco foram tombados a partir de iniciativa de pesquisadores a fim de evitar que as consequências do momento que o Brasil estava passando e a busca de um progresso que vê o patrimônio cultural como um empecilho os atingisse e que se perdessem alguns dos locais representativos da história da Restauração Pernambucana. Entretanto, a preservação desse patrimônio arqueológico necessita de ações conjuntas e efetivas com a participação da população para que se perdure para as futuras gerações. / The present study aimed to analyze the factors of influence on the processes of tipping and its consequences in the preservation of heritage material and archaeological in four parks of the metropolitan area of Recife. The question of the search for a national memory lived on the decades of 1960 to 1980 here in Brazil has exercised a strong influence on the choices of which assets constituted examples of the Union’s forming identity. Using the processes of tipping as guiding, we analyzed the tipping strategies held in Pernambuco, we evaluated the political discourse, that influenced these ideological processes tipping and, finally, we analyzed the strategies of tipping and preservation the archaeological heritage of historical and/or archaeological parks of the country where the archaeological factor was the main reason for the tumbling process. For this, designed a protocol for analysis of technical, legal and social dimensions. And, from that we highlighted five key elements that allowed us to relate the speeches and actions the preservationists that have submitted these assets. Thus, we obtained that the historical parks in Pernambuco were listed from researchers’ initiative in order to avoid the consequences of the moment that the Brazil was going through and the search of a progress that see the cultural heritage as a trammels that hit and that lost some of the local representative of the history of Pernambuco's Restoration. However, the preservation of this archaeological heritage requires joint and effective actions with the participation of the population to which they will endure for the future generations.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2015-12-02T17:13:26Z No. of bitstreams: 1 emanueldealmeidagoncalves.pdf: 1150968 bytes, checksum: 98426c572bddae595756f983af56a97b (MD5) / Rejected by Adriana Oliveira (adriana.oliveira@ufjf.edu.br), reason: Título em maiúsculo on 2015-12-03T11:32:45Z (GMT) / Submitted by Renata Lopes (renatasil82@gmail.com) on 2015-12-03T11:41:54Z No. of bitstreams: 1 emanueldealmeidagoncalves.pdf: 1150968 bytes, checksum: 98426c572bddae595756f983af56a97b (MD5) / Rejected by Adriana Oliveira (adriana.oliveira@ufjf.edu.br), reason: Palavras-chave: primeira letra de cada palavra em maiúsculo. Acrescentar uma em cada linha (não precisa colocar ponto e nem vírgula no final) on 2015-12-03T11:59:57Z (GMT) / Submitted by Renata Lopes (renatasil82@gmail.com) on 2015-12-03T13:37:12Z No. of bitstreams: 1 emanueldealmeidagoncalves.pdf: 1150968 bytes, checksum: 98426c572bddae595756f983af56a97b (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2015-12-03T13:58:56Z (GMT) No. of bitstreams: 1 emanueldealmeidagoncalves.pdf: 1150968 bytes, checksum: 98426c572bddae595756f983af56a97b (MD5) / Made available in DSpace on 2015-12-03T13:58:56Z (GMT). No. of bitstreams: 1 emanueldealmeidagoncalves.pdf: 1150968 bytes, checksum: 98426c572bddae595756f983af56a97b (MD5) Previous issue date: 2015-02-25 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O conhecimento da distribuição de insetos aquáticos é de grande importância para a conservação da fauna bentônica. O objetivo do estudo foi avaliar a composição e a distribuição espacial da fauna de Ephemeroptera, Plecoptera e Trichoptera (EPT) em oito córregos florestados no sudeste de Minas Gerais. As coletas foram realizadas no período seco de 2010, sendo obtidas 10 amostras de folhiço em corredeira e 10 em remanso de cada córrego com Surber. Foram identificados 5631 organismos e 49 gêneros de EPT. Os valores de abundância e a composição da fauna diferiram entre os córregos e mesohabitats, enquanto a riqueza diferiu apenas entre os riachos. Smicridae, Phylloicus, Leptophlebiidae sp1, Paragripopteryx e Anacroneuria foram os táxons que mais contribuíram para a separação dos córregos. Doze táxons foram registrados em maior abundância e freqüência em corredeiras e seis em remansos. Os resultados do estudo sugerem que características em escala de córregos (temperatura da água, por exemplo) e de mesohabitat (heterogeneidade do folhiço e velocidade da água) são determinantes da variação na estrutura e composição das assembléias de EPT. Palavras-chave: / The knowledge of the distribution of aquatic insects is of great importance for conservation. The objective of the study was to evaluate the composition and spatial distribution of Ephemeroptera, Plecoptera and Trichoptera (EPT) fauna, in eight forested streams in southeastern Minas Gerais. Samples were collected during the dry season of 2010, and obtained 10 litter samples in riffles and 10 in pools of each stream with Surber. 5631 organisms and 49 genera of EPT were identified. The values of abundance and assemblage composition differed between streams and mesohabitats, while richness differed only between streams. Smicridae, Phylloicus, Leptophlebiidae sp1, Paragripopteryx and Anacroneuria were the taxa that most contributed to the separation of streams. Twelve taxa were recorded in greater abundance and frequency in riffles and six in pools. The study results suggest that features scale streams (water temperature, for example) and mesohabitat (heterogeneity of litter and water velocity) are determinants of variation in the structure and composition of the EPT assemblages. Keywords: Preservation

O presente estudo teve como objetivo avaliar a ação do campo magnético pulsado sobre as propriedades físico-químicas e microbiológicas do caldo de cana armazenado sob refrigeração. As amostras de caldo de cana foram expostas ao campo magnético pulsado nas frequências de 40 Hz e 60 Hz por 30 e 210 minutos, e na frequência de 50 Hz por 2 horas. Amostras controles não foram expostas ao campo magnético. As amostras foram armazenadas por até 6 dias a 4ºC e analisadas no tempo de armazenamento de 6, 12, 24, 48, 96 e 144 horas por meio de análises físico-químicas para todas as amostras, e microbiológicas para as amostras tratadas com campo magnético de 50 e 60 Hz. O campo magnético de 40 Hz aumentou os parâmetros de cor L* e a* e diminuiu o parâmetro b*, efeito contrário ao campo de 60 Hz. O tratamento a 50 Hz aumentou o parâmetro de cor L* e diminuiu os parâmetros a* e b*. A exposição ao campo magnético de 50 Hz e 60 Hz reduziu o crescimento de bactérias totais, sendo maior a redução nas amostras tratadas a 50 Hz, e para as amostras de 60 Hz, quanto maior o tempo de tratamento, maior a inibição do crescimento microbiano. O tratamento por campo magnético de 60 Hz por 30 minutos reduziu a atividade antioxidante e a concentração de compostos fenólicos, enquanto que o tratamento a 40 Hz por 30 minutos reduziu somente o primeiro índice e a 60 Hz por 210 minutos reduziu o segundo. Os demais parâmetros físico-químicos não sofreram alteração significativa com relação ao controle durante o armazenamento. Conclui-se que, apesar de serem necessários mais estudos para entendimento do mecanismo de ação do campo magnético em alimentos, o campo magnético pulsado de baixa intensidade (ordem de mili Tesla) possui potencial para ser utilizado como tecnologia inovadora no tratamento de alimentos para controle microbiológico. / This study aimed to evaluate the action of pulsed magnetic field in the physicochemical and microbiological properties of sugar cane juice during refrigerated storage. Sugar cane juice samples were exposed to pulsed magnetic field at frequencies of 40 and 60 Hz for 30 and 210 minutes and at frequency of 50 Hz for 120 minutes. Control samples were not exposed to magnetic field. Samples were stored at 4ºC for up to 6 days, and evaluated at times of storage of 6, 12, 24, 48, 96 e 144 hours through physicochemical analysis for all samples and through microbiological analysis for samples treated with 50 and 60 Hz magnetic field. Exposure to 40 Hz magnetic field raised the color parameters L* and a*, and decreased the b* parameter, while de 60 Hz magnetic field had the opposite effect. The treatment with 50 Hz magnetic field raised the color parameter L* and decreased a* and b* parameters. Exposure to 50 and 60 Hz magnetic field reduced the growth of total bacteria, where the frequency of 50 Hz was more effective. The treatment with 60 Hz magnetic field for 30 minutes reduced the antioxidant activity and the total phenolic compound, while the 40 Hz treatment for 30 minutes reduces only the first analysis and at 60 Hz for 210 minutes reduced only the second. The other physicochemical properties did not changed significantly during storage compared with the controls. We concluded that, although more studies is needed for better understand the action mechanism of magnetic field in foods, the low intensity pulsed magnetic field (order of mili tesla) has the potential to be used as innovative technology in food treatment for microbiological control.

O meio ambiente dos museus, arquivos e bibliotecas, bem como os materiais desses ambientes como o papel e o couro, são favoráveis e preferenciais aos agentes biológicos que, destroem os registros dos documentos em pouco tempo associados aos agentes químicos e físicos. Numerosos estudos experimentais têm mostrado, efetiva ação de alterações ambientais para o controle das infestações e proteção de livros e arquivos documentais que têm sido atacados por agentes bibliófagos como insetos, fungos e bactérias. A relevância do tema reside na importância da preservação de documentos históricos, base da identidade de uma cultura e desenvolvimento da ciência e da tecnologia. Deter a ação da degradação da documentação histórica sem o comprometimento causado pela utilização de produtos tóxicos, submetendo o material do acervo a um ambiente profilático à contaminação controlando as condições de armazenamento, manuseio, uso e assepsia de modo a preservar o acervo bibliográfico com menor impacto ambiental além de aumentar a permanência dos documentos originais. Este trabalho sobre conservação de acervos faz uma abordagem histórica a respeito da invenção e da evolução do papel como suporte da escrita e descreve alguns princípios conceituais referentes ao modo de como podemos controlar o ambiente dos Acervos Bibliográficos para dificultar a degradação do material de sua responsabilidade além de causar menor impacto ao ambiente. São apresentadas soluções simples para os problemas concernentes a deteriorações e desastres a que estão sujeitos os acervos constituídos em sua maioria por material orgânico além de descrever as principais técnicas e procedimentos de conservação adotados composto de cinco tratamentos técnicos: fumigação, câmara anóxia, higienização, uso de radiação gama e acondicionamento de obras. / The environment of the museums, files and libraries, as well as the materials of these places as the paper and the leather is favorable and preferential to the biological agents that destroy the registrations of the documents in a short time associated to the chemical and physical agents. Several studies have been showing environmental alterations in an effective way in the control of the infestation and documentary book protection and files that have been attacked by damage agents as insects, fungi and bacterias. The relevance of the subject is the importance to preserve the historical documents that is the identity of a culture and development the science and the technology. To stop the degradation of the historical files without problems caused by the use of poisonous products submitting the material of the archival to a prophylactic environment to the contamination controlling its conditions like the handle, the use and the way to preserve the archives with the least environmental impact besides increasing the permanence of the original documents. This work related to the archives conservation makes a historical approach about the invention and the evolution of the paper as a writing support and it describes some concepts related to the way we can control the environment of the archives to make difficult the degradation of its material besides causing a little impact in these places. Simple solutions are presented for the problems to deteriorations and disasters that they are subject to the archivals constituted in most cases by organic material besides describing the main techniques and the conservation procedures composed of five technical treatments: fumigation, anoxia chamber, hygienic cleaning, and the use of gamma rays and conditioning of works.

Introduction : La prise de décision concernant la préservation de la fertilité dans un contexte de prise en charge urgente de cancer du sein est complexe. Les objectifs de ce travail étaient de définir les attentes et les besoins des patientes en matière d’information, de concevoir un outil d’aide à la décision en ligne et d’évaluer sa validité apparente et son influence sur le choix éclairé des patientes.Méthodologie : Trois méthodes de recherche ont été menées pour répondre aux hypothèses : un focus group de cinq patientes pour l’étude qualitative, l’élaboration d’un outil d’aide à la décision selon la méthode d’Ottawa et une étude randomisée monocentrique. Cette dernière a été menée auprès de patientes âgées de 18 à 40 ans, adressées pour préservation de la fertilité dans un contexte de cancer du sein, randomisées au sein du groupe IRIS (information standard) ou du groupe DECISIF (avec outil d’aide à la décision en ligne). La mesure du choix éclairé était évaluée selon trois critères, à l’aide d’un questionnaire : les connaissances, l’attitude, et la mise en place ou non de techniques de préservation de la fertilité.Résultats : L’étude qualitative a permis de mettre en évidence que l’information transmise aux femmes et l’annonce de l’infertilité potentielle était à parfaire. L’outil d’aide à la décision en ligne a été élaboré suivant les recommandations des patientes et des professionnels de santé impliqués qui ont confirmé sa validité apparente. En ce qui concerne l’étude quantitative, au total 125 patientes ont été inclues et randomisées dans le groupe IRIS (n=65) ou DECISIF (n=60). Il a été mis en évidence une amélioration des connaissances dans le groupe DECISIF (8.6/10 (±1.34)) par rapport au groupe IRIS (6.49/10 (±1.89)). Dans les deux groupes, les patientes ont une attitude favorable envers la préservation de la fertilité (96 %). La décision finale n’est pas influencée par l’appartenance à un groupe ou un autre. Ainsi, à l’issue de la consultation de préservation de la fertilité, 73.6 % (92/125), soit 69.2 % (45/65) des patientes du groupe IRIS et 78.3 % (47/60) des patientes du groupe DECISIF vont choisir de conserver leurs ovocytes, embryons et/ou tissu ovarien. La proportion de choix éclairé était statistiquement plus élevée dans le groupe DECISIF que dans le groupe IRIS (respectivement 75 % versus 38.5 %, p<0.001). Par ailleurs, le niveau de conflit décisionnel chez les patientes du groupe DECISIF était moins élevé que chez celles du groupe IRIS (respectivement 14.4 (±2.94) versus 15.1 (±2.18), p=0.13).Conclusion : Nous avons conçu un outil d’aide à la décision en ligne pour accompagner les femmes atteintes d’un cancer du sein dans leur prise de décision concernant la préservation de la fertilité. Notre travail de recherche a permis de valider scientifiquement cet outil qui améliore les connaissances des patientes et leur autonomie de décision sans influencer leur attitude envers la préservation de la fertilité. / Introduction: Decision-making regarding fertility preservation in the context of urgent breast cancer management is complex. The objectives of this study were to define patients' expectations and information needs, develop an online decision support tool, and assess its apparent validity and influence on patients' informed choice.Methodology: Three research methods were used to address the hypotheses: a focus group of five patients for the qualitative study, the development of a decision support tool using the Ottawa method and a randomized, single-centre study. The latter was conducted with patients aged 18 to 40 years referred for fertility preservation in the context of breast cancer, randomized to the IRIS group (standard information) or the DECISIF group (with online decision support tool). The measurement of informed choice was evaluated according to three criteria using a questionnaire: knowledge, attitude, and whether fertility preservation techniques were available.Results: The qualitative study revealed that the information provided to women and the announcement of potential infertility needed to be improved. The online decision support tool was developed based on recommendations from the patients and health professionals involved, who confirmed its apparent validity. For the quantitative study, a total of 125 patients were included and randomized to the IRIS (n=65) or DECISIF (n=60) group. Better knowledge was found in the DECISIF group (8.6/10 (±1.34)) compared to the IRIS group (6.49/10 (±1.89)). In both groups, patients had a positive attitude towards fertility preservation (96%). The final decision was not influenced by membership in one group or another. Thus, at the end of the fertility preservation consultation, 73.6% (92/125), i.e. 69.2% (45/65) of patients in the IRIS group and 78.3% (47/60) of patients in the DECISIF group, chose to keep their oocytes, embryos and/or ovarian tissue. The proportion of informed choice was statistically higher in the DECISIF group than in the IRIS group (respectively 75% versus 38.5%, p<0.001). In addition, the level of decision-making conflict among patients in the DECISIF group was lower than in the IRIS group (respectively 14.4 (±2.94) versus 15.1 (±2.18), p=0.13).Conclusion: We have developed an online decision support tool to assist women with breast cancer in making decisions about fertility preservation. We have now validated this tool, which improves patients' knowledge and decision-making autonomy without influencing their attitude towards fertility preservation.