The zygotic germplasm of plant species producing desiccation-sensitive seeds can be conserved in the long-term only by cryopreservation. Usually the embryonic axis is excised from the cotyledons and is used as the explant for cryopreservation as it is small and provides a large surface area:volume ratio. However the shoot of the axis of most species studied does not develop after excision, with the result that survival after cryopreservation is often recorded as callus production or simply explant enlargement and/or greening. Thus, besides explant size, factors such as in vitro regeneration techniques, physical injury induced upon excision and developmental status of the seed could compromise the success of cryopreservation. This study investigated the effect of the factors mentioned above, with particular attention to the developmental status of the seeds on explant in vitro development (section 3.1), response to dehydration (section 3.2) and cryopreservation of the desiccation-sensitive embryonic axes (section 3.3) of two species: Trichilia dregeana, T. emetica and embryos of a third, Strychnos gerrardii. For all three species, investigations were conducted on the embryonic axes/embryos excised from mature seeds immediately after fruit harvesting and from mature seeds stored under hydrated conditions for different periods (in order to achieve different degrees of development). In addition, preliminary studies were carried out on axes of T. dregeana to assess whether generation of reactive oxygen species (ROS) occurs in response to wounding upon axis excision (section 3.4). Excised embryonic axes of T. dregeana and T. emetica did not develop shoots in vitro unless the explants included attached cotyledonary segments. Following the development associated with short-term storage, however, the excised axes could develop shoots after complete cotyledon excision. The embryos from the (endospermous) seeds of S. gerrardii which included the paper-thin cotyledons, developed normally in vitro, with percentage germination increasing with seed storage time. For all three species, in vitro axis germination was promoted when activated charcoal was included in the germination medium, regardless of the developmental stage of the seeds. When dehydrated to approximately 0.3 g H2O g-1 dry mass (g g-1), embryonic axes from all three species failed to develop shoots even though a minimum of 50% produced roots in all cases. Hence, shoot production was shown to be more sensitive to desiccation than was root production. Furthermore, the sensitivity of the shoot apical meristem to desiccation was not ameliorated with seed storage for T. dregeana and T. emetica, but did decrease for S. gerrardii when seeds were stored for 6 – 8 weeks. The application of certain cryoprotectants did facilitate production of shoots after dehydration by a few axes of both Trichilia spp. For T. dregeana explants, combination of glycerol and sucrose allowed for 10% of the axes to retain the ability for shoot production after dehydration while for T. emetica explants, the combination of DMSO and glycerol (10 - 20% shoot production after dehydration) was best. The efficacy of the cryoprotectants was not influenced by storage period. The provision of cryoprotectants still needs to be tested for S. gerrardii. Survival of subsequent cryopreservation of T. dregeana and S. gerrardii explants was best achieved with rapid cooling in nitrogen slush, with the cooling procedure for T. emetica explants still to be optimized. The highest post-cryopreservation survival of T. dregeana axes was achieved when seeds had been stored for three months, while the seed storage period did not affect post-thaw survival of the axes of T. emetica or S. gerrardii. A small proportion of S. gerrardii explants only, produced shoots after cryopreservation, whereas the surviving embryonic axes of T. dregeana and T. emetica regenerated only as non-embryogenic callus. Although callus production is less desirable than successful seedling establishment, it has the potential for micropropagation if embryogenicity can be induced. Ultrastructural examination of the shoot apical meristem of T. dregeana after a 3-d recovery period, following excision, revealed considerable cellular derangement, although damage of individual organelles could not be resolved microscopically. Preliminary studies on T. dregeana involving a colorimetric assay using epinephrine, confirmed the generation of ROS in response to wounding associated with axis excision. Reactive oxygen species generated appeared to persist over prolonged periods rather than occurring only as a single oxidative burst. Hence, ROS production at the wound site could be the primary factor contributing to lack of shoot development. Axes immersed in the anti-oxidant, ascorbic acid (AsA) immediately after excision, showed lower ROS production and 10% shoot development when cultured in vitro, indicating that the oxidative burst coincident with, and after excision might be counteracted if immediate ROS production can be adequately quenched. Future investigations should aim to identify the specific ROS associated with wounding and optimize an anti-oxidant treatment(s) that will facilitate shoot development. Thus, the successful cryopreservation of the germplasm of the species tested, and others producing recalcitrant seeds, depends on a spectrum of species-specific factors, some still to be elucidated. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2007.

An analysis of why cultural heritage sites are created, preserved, and developed is what concerns the pages of this study. It identifies patterns and shifts in cultural heritage preservation in the period between 1977 and 1999 in KwaZulu-Natal, and analyses the motivations for the preservation of cultural heritage. Using specific case studies, I argue that in KwaZulu-Natal political necessities and ideas of economic development largely motivated cultural heritage preservation. I also examine the (dis)connection between academic historians and cultural heritage preservation. I indicate that their (dis)connection with cultural heritage preservation, especially its motivations, was a complex one. I argue that in complex ways some academic historians were drawn into the tendencies that were characteristic of cultural heritage presentations of history in KwaZulu-Natal during this period. / Thesis (M.A.)-University of Natal, Pietermaritzburg, 2001.

1918-1940 m. Lietuvoje lenkų tautinė mažuma aktyviai organizavo ir plėtojo kultūrinę veiklą. Viena iš intensyvių ir kartu labai reikšmingų lenkiško kultūrinio gyvenimo išraiškų buvo teatras. Lietuvos lenkai, turėję du draminio pobūdžio mėgėjiškus teatrinius kolektyvus Kaune ir po vieną Panevėžyje ir Ukmergėje, nuolat statė spektaklius pagal įvairaus pobūdžio repertuarą. / During the years of 1918 – 1940, the Polish minority was active in organizing and spreading cultural activities in Lithuania. One of the most intensive and in the same time a very significant Polish cultural expressions was the theatre. The Lithuanian Polish had two amateur drama type theatre companies in Kaunas and one each in Panevėžys and Ukmergė. They were constantly staging plays for a various types of repertoire.

According to the 1990 bulletin issued by the National Park Service, traditional cultural properties (TCPs) derive their significance from cultural practices or beliefs of living communities. This thesis centers on a case study of the nomination of Casita Rincón Criollo to the National Register of Historic Places as a TCP. The nomination is a collaborative project of Place Matters in New York City and Western Kentucky University, initiated by the American Folklore Society Working Group in Folklore and Historic Preservation Policy. Casita Rincón Criollo has several issues that make nomination to the National Register tricky. Casitas are small “houses,” typically accompanied by gardens, which serve as community gathering places for the Puerto Rican community in New York City. Often built illegally on empty lots, casitas tend to be impermanent structures. Casita Rincón Criollo in the South Bronx is less than 50 years old and has been moved and reconstructed. However, such is the nature of casitas. Building, maintaining, and rallying to save and move the casita makes the Casita Rincón Criollo significant. Further, Casita Rincon Criollo has served as a key influence on traditional forms of Puerto Rican music in the United States. For this reason, the Casita is recognized on City Lore’s grassroots register, Place Matters, and it was also incorporated into the GreenThumb garden movement in NYC. Folklorists are uniquely poised to recognize cultural groups and communities that might otherwise be overlooked by the National Register of Historic Places. In this thesis, I will discuss methods of research employed in the documentation of Casita Rincón Criollo and examine how folkloristic methods can address gaps in representation. I will contextualize the project within a broader history of heritage designation programs in the United States and world. From ethnographic fieldwork, oral histories, and more, I will conclude that folklorists offer alternative documentation strategies to supplement those most commonly employed in National Register nominations, as well as a more inclusive definition of cultural groups and tradition.

Recent reports detail a rise in the practice of home food preservation in the United States due to economic woes, nutritional concerns, and increasing devotion to local food production.Home food preservation is the processing of foods in order to extend its shelf-life. Current common approaches to preserving foods at home include pressure canning, freezing, drying, water bath canning, and cellaring/storing. Local food production in four Kentucky counties were examined through in-depth qualitative interviews with home food preservation practitioners to yield a rural/urban comparison. Forty home food preservation practitioners were interviewed between Fall 2009 and Fall 2013. The primary question driving this project is what motivates those who grow gardens and practice home food preservation in an era of readily available, relatively cheap foodstuffs? Secondary questions include, how do the motivations of home food preservation practitioners compare in rural and urban areas? What are the links, if any, between home food preservation and environmental sustainability concerns in rural and urban areas? Each of these questions will be examined through a mixture of qualitative methods and a grounded theoretical approach. In-depth field interviews with 40 preservers, documentary filmmaking, and participant observation were conducted in two rural and two urban Kentucky counties. Interview transcripts were coded by themes, interpreted using hermeneutic analysis, and analyzed by grounded theory. Policy institutes could make gains from this research by building upon already existing community food practices. Agriculture extension agent could use these findings to inform their food preservation programs and improve safety recommendations.

Motivated by increasing public awareness of possible abuse of confidential information, which is considered as a significant hindrance to the development of e-society, medical and financial markets, a privacy preserving data mining framework is presented so that data owners can carefully process data in order to preserve confidential information and guarantee information functionality within an acceptable boundary. First, among many privacy-preserving methodologies, as a group of popular techniques for achieving a balance between data utility and information privacy, a class of data perturbation methods add a noise signal, following a statistical distribution, to an original numerical matrix. With the help of analysis in eigenspace of perturbed data, the potential privacy vulnerability of a popular data perturbation is analyzed in the presence of very little information leakage in privacy-preserving databases. The vulnerability to very little data leakage is theoretically proved and experimentally illustrated. Second, in addition to numerical matrices, social networks have played a critical role in modern e-society. Security and privacy in social networks receive a lot of attention because of recent security scandals among some popular social network service providers. So, the need to protect confidential information from being disclosed motivates us to develop multiple privacy-preserving techniques for social networks. Affinities (or weights) attached to edges are private and can lead to personal security leakage. To protect privacy of social networks, several algorithms are proposed, including Gaussian perturbation, greedy algorithm, and probability random walking algorithm. They can quickly modify original data in a large-scale situation, to satisfy different privacy requirements. Third, the era of big data is approaching on the horizon in the industrial arena and academia, as the quantity of collected data is increasing in an exponential fashion. Three issues are studied in the age of big data with privacy preservation, obtaining a high confidence about accuracy of any specific differentially private queries, speedily and accurately updating a private summary of a binary stream with I/O-awareness, and launching a mutual private information retrieval for big data. All three issues are handled by two core backbones, differential privacy and the Chernoff Bound.

The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long term-preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature. In the present research, we have accomplished a year-long study to investigate the effect of extreme climatic conditions on the stability of spores-based whole-cell biosensing systems. The spores were stored in laboratory conditions that simulated those found in real harsh environments and germination ability and analytical performance of the spore-based sensing systems upon storage in such conditions was monitored. Our results proved that the intrinsic resistance of spores to harsh environmental conditions helped maintain the integrity of the sensor bacteria. The revived active cells actually retained their analytical performance during the course of the twelve-month storage study.

Diese Arbeit besteht aus drei Teilen. Im ersten Teil wurde Holz mit verschiedenen, kommerziell erhältlichen Kieselsolen behandelt, die unterschiedliche pH-Werte und Oberflächenmodifikationen aufwiesen. Basische Kieselsole vermochten nicht in das Holz einzudringen, da ihr pH-Wert während des Eindringens absinkt und es zur Ausfällung des Kieselsols im Holz kommt. Neutrale und saure Kieselsole hingegen konnten problemlos in das Holz eingebracht werden. Eines der sauren Kieselsole, welches mittels Aluminumoxychlorid kationisch modifiziert war, reduzierte die Wasseraufnahme und den pilzlichen Abbau durch die Braunfäule Coniophora puteana (Kiefer) und die Weißfäule Trametes versicolor (Buche). Im Bläuetest zeigte sich ein verminderter Befall durch Aureobasidium pullulans, allerdings kein kompletter Schutz gegen diesen Pilz. Auch die kleinsten verfügbaren Partikelgrößen für Kieselsole ergaben keinerlei Zunahme des Zellwandvolumens (chemische Quellung, Bulking), was darauf hinweist, dass eine Eindringung in die Zellwand nicht stattfand. Es erscheint daher nicht möglich, Kieselsole in die Zellwand einzubringen und die Dimensionsstabilität des Holzes zu verbessern. Da Kieselsol lediglich in die Lumen der Holzzellen eingebracht werden kann, kann die Behandlung nicht als wirkliche Holzmodifizierung angesehen werden. Wegen der vielversprechenden Ergebnisse in den Wasseraufnahmeversuchen und den Pilztests wurde mit dem kationischen Kieselsol behandeltes Holz thermogravimetrisch und in einem Brandtest untersucht. Im thermogravimetrischen Test zeigte sich eine leicht verminderte Pyrolysetemperatur (eine übliche Wirkung von Feuerschutzmitteln), die Holzkohlemenge war jedoch nicht erhöht. Dies zeigt, dass die Menge an brennbaren Gasen, die während der Pyrolyse freiwerden, durch das Kieselsol nicht vermindert wurde. Auch zeigte die resultierende Holzkohle gleiche Oxidationseigenschaften wie die Holzkohle der Kontrollen. Im Brandtest wurden die Branddauer, die Brandgeschwindigkeit und der Gewichtsverlust vermindert. Das Nachglühen der Holzkohle wurde komplett unterbunden. Alle diese Effekte waren jedoch relativ klein verglichen mit den Effekten eines kommerziell erhältlichen Feuerschutzsalzes, welches ebenfalls als Referenzbehandlung getestet wurde. Im zweiten Teil der Arbeit wurden acetoxyfunktionelles Silan und verschiedene Polydimethylsiloxane (PDMS) mit Acetanhydrid kombiniert, um Holz zu acetylieren. Die PDMS hatten die folgenden Funktionalitäten: Amino, Acetoxy, Hydroxy und nicht-funktionell. Die beste Hydrophobierung des acetylierten Holzes wurde durch die Kombination mit acetoxyfunktionellem PDMS erreicht, welches anschließend in verschiedenen Konzentrationen getestet wurde. Eine Konzentration von 1% in Acetanhydrid zeigte bereits eine maximale Hydrophobierung, welches darauf schließen lässt, dass die inneren Oberflächen des Holzes mit dem PDMS belegt und hydrophobiert wurden. Die Pilzresistenz des behandelten Holzes wurde durch die Kombination mit dem PDMS nicht beeinflusst. Bei Wasserlagerung zeigte sich eine leichte Überquellung des Holzes, welches mit der Kombination von Acetanhydrid und PDMS acetyliert worden war. Untersuchungen der Biegefestigkeit und Bruchschlagarbeit ergaben jedoch keinen Einfluss. Im dritten Teil der Arbeit wurden wasserbasierte Emulsionen von funktionellen PDMS zur Imprägnierung von Holz eingesetzt. Es wurde untersucht, ob Resistenz gegen pilzlichen Abbau und Hydrophobierung wie auch erhöhte Dimensionsstabilität mit dieser Behandlung erreicht werden kann. Die α-ω-gebundenen Funktionalitäten der PDMS waren: Amino, Carboxy, Epoxy und Carbobetain. Die stärkste Hydrophobierung wurde mit dem carbobetain-funktionellen PDMS erreicht, allerdings ergab diese Behandlung keine verbesserte Pilzresistenz gegenüber einem Abbau durch Coniophora puteana und Trametes versicolor. In dieser Hinsicht die beste Wirkung zeigte die Behandlung mit carboxy-funktionellem PDMS. Dieses Material verminderte jedoch die Wasseraufnahmerate nur ungenügend und wurde außerdem stark ausgewaschen. Daher wurden in der Folge amino-funktionelles und carboxy-funktionelles PDMS kombiniert, um durch eine Salzbildung der beiden Funktionalitäten eine verbesserte Fixierung des carboxy-funktionellen Siloxans zu erreichen. Die Kombination zeigte bei einem Überschuss an amino-funktionellem PDMS eine gute Fixierung, jedoch ansonsten keine Synergieeffekte. Die Dimensionsstabilität des Holzes wurde durch die Behandlungen nur sehr geringfügig verbessert. Hierfür müsste eine gute Eindringung der Chemikalien in die Zellwand erfolgen und ein dauerhaftes Bulking erzielt werden. Die Eindringung der Chemikalien in die Zellwand war jedoch in allen Fällen nur gering.

For over forty years, archivists and records managers have developed and advocated numerous theories and methodologies for the preservation of authentic and reliable organizational digital records. Many different tools, standards, and guidelines have been created to enhance and safeguard the content and context of digital records across multiple migrations. In addition, several archives have been able to create and sustain full-scale digital preservation programs. However, in spite of these successes, most archives and records management programs are not yet capable of preserving digital records indefinitely. Long-term archival digital preservation advocacy is defined as efforts undertaken by archivists and records managers to convince others, inside and outside their professions, to support the concepts, methodologies, and resources necessary to implement long-term digital preservation. Advocacy as a political- and policy-focused activity to convince a targeted audience to act is distinguished from archival “public programming” that is centered around outreach, publicity, exhibitions, and reference services. Advocacy for long-term digital preservation has not been widely successful, as evidenced by the relative small number of fully supported and fully functional archival digital preservation programs. As a result, the potential for a “dark age” characterized over the long term by a dearth of surviving, readable, and contextualized digital records is very real. This thesis explores why advocacy efforts have created only limited success, as well as what might be done to improve this situation. It rethinks long-term digital preservation as an issue of advocacy and will, as well as one of technology, strategy, or theory. Chapter One opens the thesis with a brief discussion of computers and digital records, placing advocacy efforts within a historical and technological context. Chapter Two presents an intellectual history of long-term digital records preservation advocacy literature and practice, demonstrating how advocacy “messages” have been formulated, disseminated, and “sold.” It also illustrates the multitude of informational resources and technological “solutions” that are now available to assist archivists and records managers in undertaking long-term digital preservation activities. Chapter Three tests the resonance of advocacy efforts through a series of surveys which I conducted with archivists and records managers from a variety of government, corporate, educational, and other institutions, as well as follow-up interviews with Manitoba-based records professionals. Survey questions were developed based on my examination of long-term digital preservation advocacy literature in Chapter Two, while interview questions were based on the responses of survey participants. Chapter Four concludes the thesis with a series of recommendations on improving long-term digital preservation advocacy. It argues for archivists and records managers to increase their personal commitment to long-term digital preservation, which includes having the will to embrace change and get started. Records professionals must also produce more practical internal guidance to assist archivists and records managers in undertaking preservation activities. In addition, the development and delivery of external advocacy “messages” must be improved, so that advocacy arguments better resonate with those responsible for funding organizational records management and archival preservation programs for digital records.

Recent archaeological discoveries at Fox Island County Park (Cochran 1978, 1979, and 1980a) have provided the impetus for the formulation of a cultural resources management plan for its archaeological resources. Several of the sites discovered have undergone intensive assessment and they, along National Register as an archaeological district. If they are to be properly protected, studied, and explained a management plan is essential. Therefore, within this thesis such a plan for the cultural resources of Fox Island County Park is proposed.The goals of the management plan shall be to provide for the preservation and protection of the resources using a conservation approach to archaeology and to interpret to the public their value and significance. Among the steps or objectives in achieving these goals are to: (1) seek the services of a professional archaeologist or establish develop 2 an archaeological oversight committee, (2) develop a preliminary research design, including the establishment of study units to facilitate the broadening of the data base, (3) encourage and organize volunteers, (4) increase the data base through comprehensive reconnaissance, (5) revised, long-term research design, (6) create an archaeological preserve, (7) monitor adverse impacts to the resources and develop appropriate preservation or conservation strategies, (8) gain public interest and provide information on the cultural heritage of Fox Island and vicinity, (9) identify sources in the community for monetary support, and (10) implement the long-term research design, revising it periodically as necessary.