The first part of the thesis comprises Chapters 1 to 40 of the novel, written under a pseudonym, followed by a synopsis of the remaining chapters, 41 to 155. The potential jacket copy will refer to the protagonists, a male and a female detective. The second part of the thesis is a critical study of the novel. Literary theory and critical methods are used to investigate the writing process and to explicate the text’s layers of meaning, not all of which were clear to the author at the time of writing. Chapter 1 considers literary and creative writing theory, paying particular attention to conceptualisations of author and reader. In Chapter 2, the chosen pseudonym is explained and compared with those of other authors; the novel’s title is also examined. Chapter 3 covers the issue of genre, looking at theories and discussing both crime novel and Gothic fiction. In Chapter 4, critical approaches to character are applied. Chapter 5 does the same with plot. Chapters 6 and 7 take account of the manifestations of power. Chapter 6 covers the body and gender, while Chapter 7 deals with race and class. As a conclusion, Chapter 8 describes how the first draft was transformed to one acceptable for publication.

This dissertation examines the process of limited political liberalisation in China by analysing the coexistence between critical journalists and the party-state under the Hu-Wen leadership. In contrast to the scholarship on authoritarianism and Chinese politics, which tends to analyse the perspectives of societal actors and the state separately from one another, this study brings the two together, unveiling the intricacies of their interactions. In the past decade, critical journalists and the party-state maintained a partnership which can be best described by a jazz ensemble metaphor. The players—critical journalists and the party-state—share a common purpose: improving their performance or governance within the existing political system. They overcome the limitations on their collaboration with ad hoc creative adjustments made in response to one another. The party-state acts as a band leader, setting the key by establishing a framework within which creative manoeuvring can take place. The study is based on unique access to politically sensitive material, including 120 in-depth interviews with critical journalists, media and crisis management experts, and government officials. It also includes multilayered textual analysis of the Chinese Communist Party journal, Qiushi, and investigative reports in two outspoken media outlets, Caijing and Nanfang Zhoumo. The data is employed to analyse the boundaries for limited political liberalisation of the media as well as how it manifests itself during major crisis events. More broadly, the dissertation draws the attention of both China and authoritarianism scholars to the significant yet neglected feature of interactive improvisation as a force that can sustain coexistence between critical actors and authoritarian states. It shows that by engaging in actor-driven analysis and illuminating the process of their interactions, we can better grasp the dynamics of authoritarianism in China and beyond. A step is made towards applying the analytical framework distilled in the China case on other authoritarian regimes by including a limited comparison to media–state relations under Gorbachev and under Putin. It shows that the variables of collaboration and improvisation are useful in explaining the different outcomes of political liberalisation reform.

There has been significant academic criticism of the international news coverage of Africa, but little or no first-hand research on the forces that create this news. This thesis draws on 51 semi-structured interviews and ethnographic work with practicing foreign correspondents in Sudan, Kenya and Uganda to explore the question: how can we explain and theorise the production of international news on East Africa? The thesis argues that Pierre Bourdieu’s Field Theory, and its analytical toolbox of ‘field’, ‘capital’ and ‘habitus’, can be meaningfully used to examine international journalistic practice. Field theory has been widely and productively used to understand domestic news production, but it has not yet been employed to empirically investigate journalistic production in the global sphere. The analysis is presented in three sections, each of which focuses on a different ‘layer’ of the international news system: the global field, where newswires compete for clients and capital; the national field ‘back home’ where traditional, nation based news outlets are based; and, finally, the local and immediate site where foreign correspondents work. Each of these layers is explored through an in depth case study of a major news producer/group of producers working in East Africa. The first and most substantial section examines the global journalistic field, and the position and practices of the Reuters newswire within this field. The second examines the foreign correspondents who report on Africa for print outlets in the UK. The final section presents two case studies of correspondents at work, negotiating a local news ecology: the election violence in Kenyan (2007-8), and the international coverage of the Darfur crisis. The discussion explores the fluidity between these three layers. Each analysis section stands alone as an investigations of major news producers in Africa today, and the forces that influence their work. Together, they build the argument that field theory is a useful approach to conceptualising the contemporary global news system, and examining journalistic practices within this. The main strengths of the theory lie in its notion of habitus; the extent to which it can incorporate and explain change; and its ability to link macro level phenomenon with micro level practice. The theory is ideally suited to capture and study the way in which foreign correspondents negotiate a complex and fluid global news system.

Executive functions, like the capacity to control and organize thoughts and behavior, develop from childhood to young adulthood. Although task switching and working memory processes are known to undergo strong developmental changes from childhood to adulthood, it is currently unknown how task switching processes are modulated between childhood and adulthood given that working memory processes are central to task switching. The aim of the current study is therefore to examine this question using a combined cue- and memory-based task switching paradigm in children (N = 25) and young adults (N = 25) in combination with neurophysiological (EEG) methods. We obtained an unexpected paradoxical effect suggesting that memory-based task switching is better in late childhood than in young adulthood. No group differences were observed in cue-based task switching. The neurophysiological data suggest that this effect is not due to altered attentional selection (P1, N1) or processes related to the updating, organization, and implementation of the new task-set (P3). Instead, alterations were found in the resolution of task-set conflict and the selection of an appropriate response (N2) when a task has to be switched. Our observation contrasts findings showing that cognitive control mechanisms reach their optimal functioning in early adulthood.

The transport of nutrients in forest soils predominantly occurs along preferential flow pathways (PFP). This study investigated the composition of phosphorus (P) forms in PFPs and soil matrix in several temperate beech forests with contrasting soil P contents in Germany. The PFPs were visualized using dye tracer experiments. Stained and unstained soil was sampled from three profile cuts per plot and analyzed for P fractions. The results show that labile P concentrations were highest in the O-layer and had the same range of values at all sites (240–320 mg·kg−1), although total P (TP) differed considerably (530–2330 mg·kg−1). The ratio of labile P to TP was significantly lower in the P-rich soil compared to the medium and P-poor soils. By contrast, the ratio of moderately labile P to TP was highest at the P-rich site. The shifts in P fractions with soil depth were generally gradual in the P-rich soil, but more abrupt at the others. The contents of labile and moderately labile P clearly differed in PFPs compared to soil matrix, but not statistically significant. The studied soils are characterized by high stone contents with low potential for P sorption. However, indications were found that labile organically bound P accumulates in PFPs such as biopores.

Extracellular matrix (ECM) composition and structural integrity is one of many factors that influence cellular differentiation. Fibronectin (FN) which is in many tissues the most abundant ECM protein forms a unique fibrillary network. FN homes several binding sites for sulfated glycosaminoglycans (sGAG), such as heparin (Hep), which was previously shown to influence FN conformation and protein binding. Synthetically sulfated hyaluronan derivatives (sHA) can serve as model molecules with a well characterized sulfation pattern to study sGAG-FN interaction. Here is shown that the low-sulfated sHA (sHA1) interacts with FN and influences fibril assembly. The interaction of FN fibrils with sHA1 and Hep, but not with non-sulfated HA was visualized by immunofluorescent co-staining. FRET analysis of FN confirmed the presence of more extended fibrils in human bone marrow stromal cells (hBMSC)-derived ECM in response to sHA1 and Hep. Although both sHA1 and Hep affected FN conformation, exclusively sHA1 increased FN protein level and led to thinner fibrils. Further, only sHA1 had a pro-osteogenic effect and enhanced the activity of tissue non-specific alkaline phosphatase. We hypothesize that the sHA1-triggered change in FN assembly influences the entire ECM network and could be the underlying mechanism for the pro-osteogenic effect of sHA1 on hBMSC.

Several studies reported training-induced improvements in executive function tasks and also observed transfer to untrained tasks. However, the results are mixed and there is a large interindividual variability within and across studies. Given that training-related performance changes would require modification, growth or differentiation at the cellular and synaptic level in the brain, research on critical moderators of brain plasticity potentially explaining such changes is needed. In the present study, a pre-post-follow-up design (N = 122) and a 3-weeks training of two response inhibition tasks (Go/NoGo and Stop-Signal) was employed and genetic variation (Val66Met) in the brain-derived neurotrophic factor (BDNF) promoting differentiation and activity-dependent synaptic plasticity was examined. Because Serotonin (5-HT) signaling and the interplay of BDNF and 5-HT are known to critically mediate brain plasticity, genetic variation in the 5-HTT gene-linked polymorphic region (5-HTTLPR) was also addressed. The overall results show that the kind of training (i.e., adaptive vs. non-adaptive) did not evoke genotype-dependent differences. However, in the Go/NoGo task, better inhibition performance (lower commission errors) were observed for BDNF Val/Val genotype carriers compared to Met-allele ones supporting similar findings from other cognitive tasks. Additionally, a gene-gene interaction suggests a more impulsive response pattern (faster responses accompanied by higher commission error rates) in homozygous l-allele carriers relative to those with the s-allele of 5-HTTLPR. This, however, is true only in the presence of the Met-allele of BDNF, while the Val/Val genotype seems to compensate for such non-adaptive responding. Intriguingly, similar results were obtained for the Stop-Signal task. Here, differences emerged at post-testing, while no differences were observed at T1. In sum, although no genotype-dependent differences between the relevant training groups emerged suggesting no changes in the trained inhibition function, the observed genotype-dependent performance changes from pre- to post measurement may reflect rapid learning or memory effects linked to BDNF and 5-HTTLPR. In line with ample evidence on BDNF and BDNF-5-HT system interactions to induce (rapid) plasticity especially in hippocampal regions and in response to environmental demands, the findings may reflect genotype-dependent differences in the acquisition and consolidation of task-relevant information, thereby facilitating a more adaptive responding to task-specific requirements.

Background According to international study results, physical therapists are afflicted with work-related musculoskeletal, psychosocial and dermal disorders as well as infections. The few existing studies in German-speaking regions focus mainly on dermal and psychosocial exposures and resulting complaints. An overview of all relevant work-related exposures and complaints of physical therapists is currently lacking. We sought to identify work-related exposures based on the subjective experiences and beliefs of physiotherapeutic representatives, in order to identify relevant work-related complaints and diseases. Likewise we aimed to compare the international evidence with the actual situation of physical therapists in Germany. Methods Two complementary qualitative approaches were used: 1) a focus group discussion with representatives of professional physiotherapy associations as well as health and safety stakeholders and 2) qualitative semi-structured telephone interviews incorporating currently employed physical therapists. The group discussion was conducted applying a moderation technique, and interviews were analyzed using the content analysis approach by Mayring. Results The focus group discussion with five participants and the 40 semi-structured interviews with physical therapists identified comparable results. The main exposures of physiotherapeutic work were considered to be musculoskeletal (e.g., awkward body postures during treatment, patient transfers, passive mobilization), psychosocial (e.g., statutory audit of prescriptions and the associated conflicts with doctors and health insurance providers) and partly dermal and infectious (e.g., wet work and risk of infection) factors. Diseases of the spine, wrist or finger joints, burnout syndrome and infections were mentioned as possible consequences. Conclusions The subjective data generated by both groups (focus group discussion and interviews) were comparable and consistent with the current state of research. The results provide new insight regarding work-related exposures and diseases of physical therapists working in Germany. These findings aided the design of a German-wide representative survey of practicing physical therapists.

Unspecific peroxygenases (UPO, EC 1.11.2.1) secreted by fungi open an efficient way to selectively oxyfunctionalize diverse organic substrates, including less-activated hydrocarbons, by transferring peroxide-borne oxygen. We investigated a cell-free approach to incorporate epoxy and hydroxyl functionalities directly into the bulky molecule testosterone by a novel unspecific peroxygenase (UPO) that is produced by the ascomycetous fungus Chaetomium globosum in a complex medium rich in carbon and nitrogen. Purification by fast protein liquid chromatography revealed two enzyme fractions with the same molecular mass (36 kDa) and with specific activity of 4.4 to 12 U mg−1. Although the well-known UPOs of Agrocybe aegerita (AaeUPO) and Marasmius rotula (MroUPO) failed to convert testosterone in a comparative study, the UPO of C. globosum (CglUPO) accepted testosterone as substrate and converted it with total turnover number (TTN) of up to 7000 into two oxygenated products: the 4,5-epoxide of testosterone in β-configuration and 16α-hydroxytestosterone. The reaction performed on a 100 mg scale resulted in the formation of about 90 % of the epoxide and 10 % of the hydroxylation product, both of which could be isolated with purities above 96 %. Thus, CglUPO is a promising biocatalyst for the oxyfunctionalization of bulky steroids and it will be a useful tool for the synthesis of pharmaceutically relevant steroidal molecules.

Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to strong fixatives and contrasting reagents for EM. Here, we show that fusion of a self-labeling protein to insulin allows for the quantification of age-distinct insulin granule pools in pancreatic beta cells by a combination of super resolution and transmission electron microscopy on Tokuyasu cryosections. In contrast to fluorescent proteins like GFP organic dyes covalently bound to self-labeling proteins retain their fluorescence also in epoxy resin following high pressure freezing and freeze substitution, or remarkably even after strong chemical fixation. This enables for the assessment of age-defined granule morphology and degradation. Finally, we demonstrate that this CLEM protocol is highly versatile, being suitable for single and dual fluorescent labeling and detection of different proteins with optimal ultrastructure preservation and contrast.