Les systèmes temps réel et distribués posent des problèmes complexes en termes de conception d'architecture et de description de comportements. De par leur criticité en vies humaines et leurs coûts de prototypage, ces systèmes ont motivé le développement d'une activité de recherche sur les langages de modélisation formelle et les techniques de validation basées modèle qui contribuent à la détection au plus tôt des erreurs de conception. Néanmoins, les langages formels ont eu un succès plus que limité dans l'industrie. L'arrivée du langage UML (Unified Modeling Language) a ouvert de nouveaux horizons pour l'intégration de langages de modélisation formelle dans une méthodologie de conception susceptible d'être mieux acceptée par les praticiens du domaine. En s'appuyant sur une expérience antérieure de la technique de description formelle Estelle et des extensions temporelles des réseaux de Petri, notre activité de recherche sur les cinq dernières années a débouché sur la production d'un profil UML nommé TURTLE (Timed UML and RT-LOTOS Environment). TURTLE surpasse UML 2.0 par ses extensions aux diagrammes d'analyse et de conception UML, sa sémantique formelle exprimée en RT-LOTOS, et ses outils de support (éditeur de diagrammes et outil de validation formelle combinant simulation et vérification basée sur une analyse daccessibilité). La méthodologie TURTLE trouve son champ d'application naturel dans la conception de systèmes temps réel et la validation d'architectures de communication en particulier. L'approche proposée a été appliquée avec succès à des systèmes satellitaires et des protocoles d'authentification.

Injuries are among the leading causes of death and disability in the world and a major public health concern. Young persons with learning disabilities have a higher rate and different pattern of injuries when compared with young persons without learning disabilities, but little is known regarding adults. The aim of this study was to determine the incidence and types of injuries experienced by a community-based cohort of adults with learning disabilities (n = 511). Face-to-face interviews were conducted with participants and their carers two years after they had first been recruited into a longitudinal study. The measures were based on those previously used with a large population-based sample (n = 6,104) in the Scottish Health Survey (2003). Results were compared between the adults with learning disabilities and the general population. Incidence of at least one injury that required medical or nursing attention or treatment in a 12-month period was 20.5% (105), of which 12.1% (62) was due to falls. The standardised incident injury ratio for adults with learning disabilities aged 18 - 64 years, compared with the regional general population aged 18 – 64 years, is 1.63 (95% confidence interval (CI) = 1.55 – 1.71). The types and causes of injuries experienced differed from those found in the general population. Incident injury was predicted by having epilepsy (odds ratio (OR) 1.809), and not having autism (OR 0.153). Incident fall injury was predicted by urinary incontinence (OR 1.976), whilst Down syndrome reduced risk (OR 0.416). Carers of adults with learning disabilities (n = 446) were less likely to experience at least one injury in a 12-month period overall, but they were significantly more likely to experience harmful injury from another person (p = 0.048), and less likely to experience injury through the use of a tool, implement or equipment (p = 0.045), when compared with the regional general population. These findings are first steps towards understanding the considerable burden of injuries, accidents and falls in the learning disabilities population, and towards informing interventions to prevent injuries and falls in adults with learning disabilities in the future. The types and causes of injury experienced by carers of adults with learning disabilities are also reported for the first time.

Recognition and education: It is important to ensure the inclusion within palliative care of chronic, progressive incurable diseases and for this additional workload to be adequately staffed and funded. There is a need to promote a clear, simple and friendly definition of Palliative Care for health care workers, patients and their carers. Education of the service providers, patients and the public to increase awareness of the palliative care needs of patients with non-cancer diseases will promote recognition and acceptance of this concept. Patient’s choice: There is a recognition among the GPs and distinct nurses for patients to be given the choice of place of care and death, and for patients and their carers to be involved in partnership in care decisions with healthcare professionals from the initial stages. This could be increased and improved if the generalist palliative care providers listened to and understood the needs of patients and their carers and promoted patient centred planning for all future strategies in palliative care. Co-ordination and communication: There is a need for improved communication and co-ordinated working between primary healthcare, social work community care, specialist palliative care teams, the voluntary sector and the acute setting. Joint working and multi-agency working groups and managed clinical networks could contribute to this. Services: Finite resources have led to service remodelling and redesign in both voluntary and statutory sectors. There were substantial changes in service provision during the study period. Over the three-year period of the study a greater number of services for patients with non-cancer were discontinued. There is a lack of specialist palliative care services in the acute sector. There is also a need for improved services including availability of hospice beds, palliative care services in care-homes and in particular social work services appear to be under provided. Health care professionals: The percentage of GPs and district nurses who had used current specialist palliative care services for their patients with cancer was more than double that of those who had used similar services for their patients with non-cancer diseases. GPs and district nurses also placed more importance on future palliative care services for their patients with cancer in comparison to similar services for their non-cancer patients. Compared to the GPs, the nurses had more contact with patients with non-cancer diseases and perhaps because of this had a greater recognition of the need for palliative care services for non-cancer patients. Service users: Patients with neurodegenerative diseases were younger compared to those with cancer. A higher percentage of patients with neurodegenerative diseases needed help with their daily activities compared to patients with cancer. There was a longer time interval between first appearance of symptoms and confirmation of diagnosis for patients with neurodegenerative diseases compared to patients with cancer. Lack of information was an issue highlighted by both groups of patients. The health status and care of the patients with neurodegenerative diseases was found to be inferior to patients with cancer. Resources: Funding was an issue identified by all service providers. Despite the desire to help with non-cancer diseases, providers of specialist palliative care and primary healthcare have their finite resources for palliative care fully utilised with cancer patients at present. The service providers agreed that they would increase their present workload, but were restricted by lack of resources. Research: There is a lack of evidence on the needs of non-cancer patients, on the best ways of meeting these needs and on the effectiveness and acceptability of services. This is necessary to provide the evidence to justify the resources (human and financial) that need to be allocated to make palliative care services to all on the basis of need and not on diagnosis

Winter rye produces ice-modifying antifreeze proteins upon cold treatment. Two of these antifreeze proteins are members of the large, highly conserved, ??-1,3-endoglucanase family. This project was designed to identify glucanase genes that are expressed during cold acclimation, wounding, pathogen infection, drought or treatment with the phytohormones ethylene and MeJa. Additionally, a more detailed proteomic analysis was to be carried out to evaluate the glucanase content of the apoplast of cold-acclimated (CA) winter rye. Results of 2D SDS-PAGE analysis revealed that non-acclimated whole leaf protein extracts contain at least two ??-1,3-endoglucanses while CA whole leaf protein extracts contain at least three ??-1,3-endoglucanses. Subsequent 2D SDS-PAGE analysis was conducted on the apoplast extracts of NA and CA winter rye plants revealed the limitations of standard 1D SDS-PAGE. The 2-dimensional gel analysis revealed that there is a minimum of 25 proteins within the apoplast of CA winter rye, including at least 5 ??-1,3-endoglucanases. Genome walking was used to isolate cold-responsive glucanase genes. The five genes isolated were designated scGlu6, scGlu9, scGlu10, scGlu11 and scGlu12. The cis-element pattern within the promoter of each gene was evaluated using online databases of documented plant cis elements. As expected, all of the promoters contained elements associated with cold, biotic and abiotic stresses, light regulation, and development. The expression patterns predicted by the cis elements in each promoter were compared to the mRNA abundance produced by each gene as detected by semi-quantitative reverse transcriptase PCR. In most cases, the abundance of transcripts arising from each gene loosely corresponded to the expression pattern predicted by the cis elements the corresponding promoter. Transcripts of scGlu9, 10 and 11 were present in cold-treated tissues and are candidates for ??-1,3-endoglucanases with antifreeze activity. The results presented in this thesis provide additional insight into the apoplast proteome of CA winter rye plants as well as the complexity of the signals controlling the proteins that reside there. Although there are still a number of unresolved questions, this research opens new directions for future studies in the cold acclimation process in winter rye and specifically for the contribution of ?? -1,3-endoglucanses.

Background: Historically, nursing has struggled to introduce researchbased interventions into routine clinical practice. Reasons for this difficulty range from poor communication between clinically and academically based nurses to limitations within organisations that obstruct the implementation of new ideas. Aims: To identify the barriers to research utilisation and the preferred methods of research dissemination amongst breast care nurses (BCNs), to develop a dissemination intervention for BCNs and to evaluate a dissemination intervention for BCNs. Method: The study was conducted in three stages. In Stage 1, a national survey was conducted using the Barriers to Research Utilisation Scale (Funk et al. 1991), questions about dissemination preferences and a demographic questionnaire. In Stage 2, the Conceptual Framework for Selecting a Targeted Experimental Dissemination Method based on social interactionalist theory was developed. In Stage 3, a pre-test/post-test randomised controlled design was used to evaluate the dissemination method developed in Stage 2; the unit of randomisation and analysis were hospital clusters of BCNs. Sample: 263 BCNs from 13 regions within the U.K. comprised the sample in Stage 1. In Stage 3, the sample consisted of 92 BCNs from 62 hospitals in the north of England. Analysis: In Stage 1 analysis was undertaken using descriptive and nonparametric statistics. In Stage 3, analysis consisted of descriptive statistics 19 and clustered regression techniques with estimation of robust standard errors: clustered logistic regression for knowledge items, clustered linear regression for knowledge scores, ologit for attitude and reported practice items and clustered multiple regression for paired and multiple variable analyses. Findings: The results from the Barriers Scale revealed ‘statistical analyses not understandable’, ‘insufficient time on the job to implement new ideas’, ‘facilities inadequate for implementation’, ‘research not reported clearly and readably’ and ‘no time to read research’ as the greatest barriers to research utilisation. Free text responses revealed additional problems with communication and conditions within provider organisations and identified facilitators for research utilisation and dissemination. These data led to the selection and production of a targeted information booklet, entitled Exercise and Breast Cancer: A Booklet for Breast Care Nurses, specifically designed to be accessible, time efficient, understandable and relevant to the target audience. In evaluation, the booklet was shown to overcome the perceived barriers of the sample associated with accessing and understanding research. A statistically significant increase in knowledge and changes of reported practice and attitudes were found. Robust variables affecting knowledge acquisition were identified as the promotion of health, promotion of exercise and understanding of how exercise can reduce cancer-related fatigue. Implications: This study has demonstrated that printed materials can be used as an effective dissemination method provided that they are developed in line with the needs, values and context of a target audience. The 20 Conceptual Framework can be followed to develop similar booklets on different topics and could provide a purposeful contribution to the promotion of evidence-based practice for all nurses.

Interferon (IFN)τ is the luteotrophic signal in ruminants and is secreted by bovine blastocysts both in vivo and in vitro. IFNτ secretion is highly variable and its control is only partly understood. Most studies on the effects of environmental factors on IFNτ production have evaluated IFNτ production during the time of embryo elongation and attachment. There is less knowledge of how IFNτ production at the blastocyst stage is modulated. Therefore, the hypothesis of this thesis was that the amounts of IFNτ expressed and/or secreted by bovine blastocysts produced in vitro were modulated by environmental factors. In the first set of experiments, bovine embryos were incubated with a cytokine (granulocyte macrophage colony stimulating factor, GM-CSF). GM-CSF had been shown previously to promote embryo viability in a range of species and to modulate IFNτ secretion by ovine blastocysts and thus was classified as a beneficial environmental factor. Three experiments were conducted to test whether GM-CSF stimulated bovine blastocyst development and IFNτ secretion. Embryos were incubated with a range of different concentrations of GM-CSF (2, 5, 10 and 50 ng mL-1) and at different stages of development (1 to 3 and 1 to 9 days post-insemination). Bovine embryos were unresponsive to GM-CSF in terms of IFNτ secretion, pyruvate oxidation, rate of development, blastocyst yield, morphological quality and apoptotic index, irrespective of timing of exposure and/or concentration of GM-CSF. In the second part of the thesis, bovine blastocysts were exposed to a mild heat treatment (42°C for four h) to determine whether heat stress affected IFNτ expression by bovine blastocysts. A novel multiplex reverse-transcription polymerase chain reaction methodology was validated to detect IFNτ and heat shock protein (HSP)70 mRNA in individual bovine embryos relative to an endogenous gene (YWHAZ) and an exogenous mRNA (α-globin) and results were expressed both in absolute terms and in relation to the endogenous control. Heat treatment upregulated IFNτ mRNA expression, suggesting that detrimental environmental factors may influence IFNτ expression. Heat treatment also caused an increase in HSP70 mRNA expression but did not affect blastocyst morphology, suggesting that the level of stress caused by the heat treatment was great enough to activate the cellular stress response, but mild enough not to cause a change in morphology. In addition, the positive correlation between HSP70 and IFNτ transcript levels and the higher IFNτ expression by embryos which showed signs of degeneration and collapse compared to those which progressed in development suggested that IFNτ expression may be indicative of stress. The relationship between IFNτ expression and secretion in vitro with morphology, pyruvate metabolism, apoptotic index and cell number was inconsistent, suggesting that IFNτ production did not correlate with ‘quality’ (defined as an index of viability). Blastocyst yield, day of blastulation and change in morphology index did account for at least part of the variation in IFNτ production, suggesting that some intrinsic factors may regulate IFNτ secretion. These intrinsic factors, however, did not explain all the variation in IFNτ secretion between blastocysts. Therefore, the amount of IFNτ secreted by bovine blastocysts is modulated by both intrinsic and environmental factors. A model was proposed where different levels of stress affect survivability to different extents, and the ability to respond to mild levels of stress may be indicative of improved survivability.

No description available.

We present an evaluation of daily estimates from three near real-time quasi-global Satellite Precipitation Products-Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA), Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks (PERSIANN), and Climate Prediction Center (CPC) Morphing Technique (CMORPH)-over the African continent, using the Global Precipitation Climatology Project one Degree Day (GPCP-1dd) as a reference dataset for years 2001 to 2013. Different types of errors are characterized for each season as a function of spatial classifications (latitudinal bands, climatic zones and topography) and in relationship with the main rain-producing mechanisms in the continent: the Intertropical Convergence Zone (ITCZ) and the East African Monsoon. A bias correction of the satellite estimates is applied using a probability density function (pdf) matching approach, with a bias analysis as a function of rain intensity, season and latitude. The effects of bias correction on different error terms are analyzed, showing an almost elimination of the mean and variance terms in most of the cases. While raw estimates of TMPA show higher efficiency, all products have similar efficiencies after bias correction. PERSIANN consistently shows the smallest median errors when it correctly detects precipitation events. The areas with smallest relative errors and other performance measures follow the position of the ITCZ oscillating seasonally over the equator, illustrating the close relationship between satellite estimates and rainfall regime.

Rondônia apresenta área com rica diversidade de artrópodes, porém pouco se conhece sobre a transmissão de arbovírus por estas espécies. O presente trabalho visou detectar arbovírus, por meio da RT-PCR e da Duplex RT-PCR, nas espécies de dipteros coletados no Estado, bem como caracterizá-los pela reação de sequenciamento. A RT-PCR e a Duplex RT-PCR detectaram as suspensões dos vírus Mayaro e Oropouche até 104 e 101 TCID50/mL, respectivamente, porém o vírus Dengue 2 em pools contendo menos de três mosquitos infectados foi negativa. O controle endógeno foi detectado em 66,8 % das amostras, sendo que, em pools contendo entre um e três mosquitos, a detecção foi aproximadamente metade da detecção nos pools contendo entre 11 e 15. Em 0,66 % dos pools foi encontrado o vírus Oropouche e em outros 0,66 %, o vírus Cacipacoré. O vírus Oropouche foi detectado em Coquillettidia sp. e Deinocerites sp., enquanto o vírus Cacipacoré foi encontrado em Anopheles sp. e Culex sp. As técnicas possibilitaram a detecção dos arbovírus pesquisados nos pools coletados em Rondônia. / The Rondônia state has an area with rich arthropods diversity although the knowledge about the arboviruses transmition for these species is poor. The present work aimed to detect arboviruses through RT-PRC and RT-PCR Duplex in the diptera species collected in the region as well as their characterization through the sequence reaction. The RT-PRC and RT-PCR Duplex detected the Mayaro and Oropouche virus suspensions until 104 e 101 TCID50/mL respectively, although it was negative for the Dengue 2 virus in pools containing less than three infected mosquitoes. The endogenous control was detected in 66,8 % of samples and from pools containing from one to three mosquitoes the detection rate was approximately half from that obtained from pools with 11 to 15 mosquitoes. Oropouche virus was found in 0,66 % of pools and Cacipacore virus also in 0,66 % of pools. Oropouche virus was detected in Coquillettidia sp. and Deinocerites sp. while Cacipacoré virus was found in Anopheles sp. and Culex sp. The techniques allowed the detection of examined arboviruses in the pools collected from Rondonia.

No description available.