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41	Anwendung des Comet Assay (Einzelzell-Gelelektrophorese) an Zellen von Fischen zum Nachweis gentoxischer Wirkungen im aquatischen Biomonitoring Nehls, Sebastian 14 October 2013 (has links) Gewässer sind Lebensgrundlage, jedoch gleichzeitig Schadstoffsenken für eine Vielzahl von Kontaminanten. Biologische Wirkungstests und das Biomonitoring aquatischer Proben sind daher besonders wichtig, um Umwelt-Gefahrenpotenziale erkennen zu können. Der "Comet Assay" (Einzelzell-Gelelektrophorese) ist ein Indikator von DNA-Strangbrüchen und wurde hier als Test auf gentoxische Wirkungen erprobt und angewandt. Mit bekannten, gentoxischen Substanzen wurden Nachweisgrenzen und Dosis-Wirkungs-Beziehungen für die Zelllinien RTG-2 und RTL-W1 (aus der Regenbogenforelle, Oncorhynchus mykiss) in vitro ermittelt und methodische Parameter an die Zellen angepasst. Der Test reagierte sehr sensitiv auf 4-Nitrochinolin-1-oxid. Die Substanz war daher geeignet, um in weiteren Versuchen als Positivkontrolle zu dienen. Zur Bewertung der Messdaten wurde ein geeignetes statistisches Verfahren gefunden, das auch historische Kontrollen mit einbezog. Der zeitliche Verlauf der DNA-Schädigung des Testsystems mit RTG-2-Zellen wurde ermittelt, und durch Inhibition der DNA-Reparatur mit Aphidicolin wurden Zusammenhänge zwischen der Entstehung von DNA-Strangbrüchen, der DNA-Reparaturkapazität sowie der Metabolisierungskapazität untersucht. In einer zweiten Phase wurden unbehandelte Wasserproben aus Rhein, Elbe sowie weitere Oberflächenwasserproben mit dem Comet Assay an RTG-2-Zellen getestet. Bei 15 von 49 Proben zeigten sich gentoxische Effekte. In einer dritten Phase wurden Erythrozyten von freilebenden Döbeln, Leuciscus cephalus, aus der Mosel mit dem Comet Assay untersucht. Die Fische von drei Messstellen zeigten erhöhte Werte von DNA-Schädigungen, gegenüber einer vierten, stromabwärts gelegenen Messstation. Korrelationen mit den Ergebnissen zusätzlicher Biomarker ergaben sich nur teilweise. Chemische Analysen von Wasser- oder Gewebeproben ließen keine Rückschlüsse auf verursachende Kontaminanten zu - gerade dies unterstreicht jedoch die Wichtigkeit biologischer Tests bei komplexen Proben. / Bodies of Water are both vital resources and pollutant sinks for a multitude of contaminants. Therefore, biological effect tests and biomonitoring of aquatic samples are of particular importance to detect potential environmental hazards. The "comet assay" (single cell gel electrophoresis) is an indicator for DNA strand breaks and was explored and applied as a genotoxicity test in the present study. Known genotoxic substances were used to determine the detection limits and dose-response relationships for the cell lines RTG-2 and RTL-W1 (from rainbow trout, Oncorhynchus mykiss) in vitro, and to adapt methodological parameters to the cells. The test was very sensitive to 4-Nitroquinoline-1-oxide. This substance was therefore well-suited to serve as positive control in further experiments. In order to evaluate the measurement data, an appropriate statistical procedure was developed, which also took "historical" controls into account. The time course of DNA damage in the test system using RTG-2 cells was determined, and relationships between the origin of DNA strand breaks, DNA repair capacity and the metabolizing capacity of the cells was investigated by means of inhibition of DNA repair with Aphidicoline. In the second stage, native water samples from the rivers Rhine and Elbe and further surface waters were tested with the comet assay, using RTG-2 cells. 15 out of 49 samples showed genotoxic effects. In a third stage, erythrocytes of feral chub, Leuciscus cephalus, from the Moselle river were examined with the comet assay. The fish from three measuring stations showed elevated values of DNA damage compared to fish sampled from a downstream station. There were only partly correlations with the results from additional biomarkers. Chemical analyses of water and tissue samples did not permit conclusions on effect-causing substances.However, this emphasizes the importance of biological tests in dealing with complex environmental samples. Comet Assay Flüsse Biomonitoring DNA-Reparatur DNA-Schäden Fische Gentoxizität Gentoxizitätstest In vitro-Test Leuciscus cephalus Mutagenität Ökotoxikologie Oncorhynchus mykiss RTG-2-Fischzelllinie RTL-W1-Fischzelllinie Umweltverschmutzung Wasserverschmutzung DNA repair Biomonitoring Comet assay DNA damage Ecotoxicology Environmental pollution Fish Genotoxicity Genotoxicity test In vitro test Leuciscus cephalus Mutagenicity Oncorhynchus mykiss Rivers RTG-2 fish cell line RTL-W1 fish cell line Water pollution 570 Biowissenschaften, Biologie 32 Biologie WC 3440 ddc:570
42	Medicínské centrum / Medical Center Pospíšil, Václav January 2020 (has links) The subject of my diploma thesis is the design of a new medical center MEDCEN in the area of the Jihlava hospital, on plot no. 4374/1. The total land area is 22 706 m2. The building has three aboveground and one underground floor. The vertical bearing structure consists of masonry ceramic elements, which are complemented by a monolithic, water-reinforced concrete in the underground floor. Vertical peripheral structures are equipped with thermal insulation system, in the above-ground floors with ventilated facade. The vertical load-bearing structures are made of prefabricated reinforced concrete panels and reinforced by reinforced concrete beams at the local load. The whole building is designed flat single-skin roof with a classic order of layers, where the main waterproofing layer consists of plasticized PVC foils. The layout of the building is divided into left and right wing, which connects the central central part. The entire underground floor is designed to be operational, there are parking spaces for employees, storage facilities and technical facilities of the building. Individual offices, nurses and waiting rooms are designed on all above-ground floors. In the central central part are designed sanitary rooms for visitors and employees. On the first floor is a pharmacy and X-ray examination room. On the third floor there are two office management offices and one-day surgery premises.
43	Zobrazování medicínských dat v reálném čase / Medical Data Rendering in Real-Time Lengyel, Kristián January 2010 (has links) This thesis deals with design and implementation of an application for medical data imaging in real-time. The first part of project is focused on methods for obtaining data in medical practice and visualization of large volume data on computer using familiar rendering approaches. Similar applications are used outside of medicine in other fields, such as chemistry to display molecular structures or microorganisms. Another part of project will focus on benefits of visualization of volumetric data using programmable hardware and new methods of parallelization of algorithms on graphics card using CUDA technology, and OpenCL. The resulting application will display the volume of medical data based on selected method accelerated by programmable shaders, and time-consuming operations will be paralleled on graphics card.
44	Vliv technologie výroby popílkového pórobetonu na vznik tobermoritických fází / The effect of fly ash aerated concrete production technology to formation of tobermoritic phases Fleischhacker, Ján January 2016 (has links) Autoclaved aerated concrete is long-time ecological building material with usefull properties. There needs to be done research of its mineralogical compound for reengineering the production. The main mineral compound of AAC is tobermorite, it increases its mechanical properties. In the presence study, we examine the usage of nature and artificial silicious materials. Also, we investigate the influence of sulfate and alumina admixtures, as well as fluidized bed combustion ash, which can be used as the lime and gypsum replacement. Closure of the study is design of the optimal raw material composition, also hydrothermal treatment of autoclaved aerated concrete. In relation to its mechanical properties, mineralogical composition and cost.
45	Studium chování vybraných hornin při působení vysokých teplot / Study of the behavior of selected rocks at high temperatures Holanec, Aleš January 2022 (has links) The diploma thesis deals with the observation and diagnostics of transformations that take place in stone slabs of different mineralogical composition during heating to high temperatures. The theoretical part of the thesis deals with a summary of available information about selected types of rocks. Furthermore, it presents findings from the experimental thermal loading of a granite sample. The principles and instruments of laboratory experimental methods that are used to judge the resistance of rocks are described in detail. In the experimental part, the testing methodology is proposed, the procedure of production of rock test specimens is described and the way in which the thermal loading of stone slabs took place is explained. The results of the experiments are shown in the tables and graphs. A comparison of individual tests and properties of selected samples and evaluation of the obtained results is performed.
46	Důsledky tvorby anortitu v keramickém střepu / Results of Anorthite Creation in ceramic Body Beránková, Karla January 2012 (has links) Anorthite is crystalline phase in the ceramic body. Is acquired on burning a mixture of calcium ceramic raw material. This work deals with the influence on the resulting properties of anorthite ceramic body, especially flexural strength, porosity, shrinkage, thermal expansion coefficient. And the influence of different CaO sources on the properties. As a source of CaO was used calcium carbonate, calcium hydroxide and fluid fly ash.
47	Zdravotnické středisko Hlinsko / Medical center Hlinsko Pilný, Ondřej January 2018 (has links) This diploma thesis deals with the design and elaboration process of a project documentation of medical center in city Hlinsko. New building is situated in the northern part of the city in the area determined for constructions of public infrastructure. It´s a four-floor object with partial basement and slant mansard and flat vegetative roof.Object is based on foundation strips and footings made from reinforced concrete.The bearing and internal walls are designed from KALKSANDSTEIN lime sand blocks.The beams and collums are used in the placed designed with open disposition.The ceiling in 1.PP is designed as reinforced concrete for securing fire safety.The rest od ceiling constructions is designed from prestressed concrete panels SPIROLL,with exception in 4.NP,where the ceiling constructions are made from solid roof system YTONG KOMFORT,which is layed on frame system made of steel and reinforced concrete.The peripheral walls of 1.PP are made from lost formwork, which is insulated with extruded polystyrene.The walls in floors above ground are insulated with combination of external thermal insulation system (ETICS) and ventilated facade with CEMBRIT SOLID and PATINA facade cladding panels. The building is functionally divided into several part.Cafe, pharmacy, ambulance and medical center, each with it´s own entrance.Pharmacy is equipped with shop, medicine mixer and storage.Ambulance is equipped with garage slots, background for employees, room of crisis preparedness and operating center.Medical centrum is equipped with emergency, RTG, operating theater, rehabilitations and other types of examination rooms.Each examination room is equipped with waiting room.For more comfort the center has a background for employees.The whole object is designed with forced air exchange. Designed object is barrier-free.In front of the building is situated parking slots for cars, motorbikes and ambulances.Total number of slots is 74 places, which 5 is designed for disabled people.
48	Lanthanide Doped Wide Band Gap Semiconductors: Intra-4f Luminescence and Lattice Location Studies / Lanthanid-dotierte Halbleiter mit großer Bandlücke: Intra-4f Lumineszenz- und Gitterplatzuntersuchungen Vetter, Ulrich 15 July 2003 (has links) No description available. Mathematics and Computer Science Lanthanide Kathodoluminesznez intra-4f Elektronenübergänge Europium Thulium Gadolinium Halbleiter mit großer Bandlücke Aluminiumnitrid kubisches Bornitrid Siliziumkarbid Diamant dünne Schichten tetraedrischer amorpher Kohlenstoff Lichtemitter Starkniveau-Spektroskopie Kristallfeldanalyse Kathodolumineszenz Energiematrix Judd-Ofelt-Theorie Ionenimplantation massenselektierte Ionenstrahldeposition Emissionschanneling Gitterplatz Mössbauerspektroskopie radioaktive Lanthanidisotope 530 Physik lanthanides cathodoluminescence intra-4f electron transitions europium thulium gadolinium wide band gap semiconductors aluminum nitride cubic boron nitride silicon carbide diamond thin films tetrahedral amorphous carbon light emitters Stark level spectroscopy crystal field analysis energy matrix Judd-Ofelt theory ion implantation mass-selected ion beam deposition emission channeling lattice location Mössbauer spectroscopy radioactive lanthanide isotopes 33.60 33.30 33.07 33.06 33.43 33.72 RTF 400: Ionenimplantation {Physik} RTG 400: Anregung von Atomen atomare Energiezustände {Atomphysik} RRJ 000: Emissions- Absorptionsspektroskopie {Physik} RVQ 000: Halbleiter {Physik}

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