The radiation-induced bystander effect is an important phenomenon in the field of radiation biology. It has been shown that cells, after exposure to radiation, can communicate with surrounding cells and affect their physiology. Otherwise-healthy recipient cells can be influenced to undergo cellular senescence or apoptosis through this process. This has potential utilizations for radiation oncology and as well as our understanding of radiation safety. The radiation-induced bystander effect has been extensively investigated since the 1990s, but the scientific community struggles to come to a unanimous decision on how strongly these signals impact the survival of bystander cells. Results show various degrees of impact on cell survival whereas certain studies refute the existence of a radiation-induced bystander effect. This may be due to the fact that there is a great deal of study heterogeneity within the radiation-induced bystander effect community. Most experiments follow a similar general bystander protocol but often use different donor and reporter cell lines that vary in sex, organ of origin, and p53 status. The type of radiation and dose rate also typically differ between experimental designs. In this analysis, 67 in vitro, medium-transfer, radiation-induced, bystander effect studies were retrospectively graphed and analyzed to determine which intrinsic and external factors contributed significantly to the overall survival percentage change observed in reporter cells. A Two-Way ANOVA was conducted on each variable and showed that the reporter cell line, p53 status, and radiation type had a statistically significant effect on survival percentage change. These findings may explain the variation in results seen in past experiments and may help standardize future research allowing for more direct comparisons. / Thesis / Master of Science (MSc)

The pro-oxidative and pro-inflammatory mechanisms have been implicated in various human diseases including neurological and psychiatric disorders. However, there is only limited information available on the etiology in the progression of neurological damage to brain. The emergence of tissue engineering with the growing interest in mechanistic studies of brain injury now raises great opportunities to study complex physiological and pathophysiological process in vitro. Therefore, the prime goals of this study include: (1) Determination of the molecular and cellular mechanisms responsible for blast- and radiation-induced brain injuries and (2) Development of a three-dimensional (3D) model system in order to mimic in vivo-like microenvironments to further broaden our knowledge in pro-oxidative and pro-inflammatory mechanisms and their cellular responses within 3D constructs. In the first study, we demonstrated that blast exposure induced specific molecular and cellular alterations in pro-oxidative and pro-inflammatory environments in the brain and neuronal loss with adverse behavioral outcome. The results provide evidence that pro-oxidative and pro-inflammatory environments in the brain could play a potential role in blast-induced neuronal loss and behavioral deficits. In the second study, we investigated that fractionated whole-brain irradiation induced specific molecular and cellular alterations in pro-oxidative and pro-inflammatory environments in the brain along with elevation of reactive oxygen species (ROS)-generating protein (NOX-2) and microglial activation. Additionally, the contribution of NOX-2 in fractionated whole-brain radiation-induced oxidative stress was observed by dramatic amelioration of ROS generation after pharmacological inhibition of NOX-2. These results support that NOX-2 may play a pivotal role in fractionated whole-brain radiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain. In the third study, we developed an in vitro 3D experimental model of brain inflammation by encapsulating microglia in collagen hydrogel with computational analysis of 3D constructs. The results indicated that our newly developed in vitro 3D model system provides a more physiologically relevant environment to mimic in vivo responses. In conclusion, these data may be beneficial in defining a cellular and molecular basis of pathophysiological mechanisms of brain injuries. Furthermore, it may provide new opportunities for preventive and therapeutic interventions for patients with brain injuries and associated neurological disorders. / Ph. D.

Head and neck cancer is a heterogeneous group of tumours, of which certain subgroups such as cancer of the mobile tongue frequently are associated with a relatively poor prognosis due to the high risk of regional failure and mortality rates that haven’t improved in a significant way over the last 3 decades, despite advancements in both diagnostics and treatment. Today we lack means to assess the biological aggressiveness of each individual tumour, which varies largely. Treatment comprises of surgery with additional radiotherapy and medical therapies in more advanced tumours. The focus in this thesis is on molecular biomarker expression in head and neck cancer and especially in association with radiotherapy. Increased knowledge paves the way to a more individualized cancer treatment aiming for better outcome and less overtreatment and sequelae. The aims of this thesis was: To map the effects of radiotherapy in both tumour and adjacent tissue for the possible markers hyaluronan, EGFR and mast cells. To investigate whether the expression of hyaluronan in the epithelium and connective tissue stroma and EGFR in the tumour correlates with the risk for developing cervical metastasis in N0 patients, and to find out whether the 3-year tumour-specific survival rates correlates with the expression of HA in the epithelium and EGFR in the tumour. To establish an animal model for radiation-induced mucositis and to use that model to examine the pattern of invading inflammatory cells. To investigate whether the expression of podoplanin in tongue cancer correlates with the risk for cervical metastasis and to determine whether the total amount of lymph vessels in the diagnostic biopsy has any impact on the clinical outcome. To investigate the differences in the metabolome of tongue cancer cell lines with different radiosensitivity. The most important findings of this thesis were: The expression of EGFR and hyaluronan hade the same pattern of expression in both tumour and adjacent tissues before radiotherapy. The expression of EGFR was increased in the epithelium of the adjacent tissue close to the tumour after radiotherapy. The intensity of the staining of hyaluronan was correlated to the 3-year survival rates in patients with tongue cancer. An experimental model for radiation-induced oral mucositis in rat was established and in this model a temporal pattern of macrophage invasion with two different subtypes of macrophages was found. There were no correlation between the expression of podoplanin in the tumour tissue and the cervical metastasis rate in patients with tongue cancer, but the younger patients were more likely to have a higher expression of podoplanin in their tumour than elder patients. Tongue cancer cell lines with different radiosensitivity respond to irradiation with different patterns of metabolic expressions.

Este trabalho apresenta uma extensiva série de resultados experimentais da condutividade induzida por radiação X contínua, durante e após a irradiação, em amostras de PET e Kapton. As medidas foram realizadas variando-se uma série de parâmetros, tais como: o campo elétrico aplicado, a taxa de exposição, a espessura da amostra, o tipo de eletrodo, a energia da radiação e as condições ambientes. Foram feitas ainda medidas da corrente termo-estimulada em amostras irradiadas e não irradiadas, que permitiram verificar a presença de armadilhas nos materiais. Medidas da corrente fotônica com diferentes eletrodos e espessuras das amostras, constataram a influência do eletrodo no valor dessa corrente. Finalmente um modelo teórico foi desenvolvido, baseado na teoria de balanço dos portadores generalizada, com a inclusão do efeito do campo elétrico na taxa de geração de portadores (efeito Onsager). O ajuste teórico-experimental permitiu a determinação numérica dos principais parâmetros de condução, tais como, mobilidade dos portadores, coeficiente de recombinação e densidade de armadilhas, para os dois materiais estudados. / In this work we present extensive results of measurements of the prompt and delayed radiation-induced conductivity of samples of PET and Kapton. Experimental parameters, such as the effective energy of the radiation, the exposure rate, the total dose, the value of the applied electric field, the nature of the electrodes, and the ambienta1 conditions were changed within wide limits. We also report measurement of thermally stimulated currents for non-irradiated and for irradiated samples which allowed us to investigate the trap-structure of the materials. Measurements of photo-Compton currents with different electrode materials and sample thicknesses gave information about the relation between the nature of the electrodes and the amplitudes of the currents. Based on the generalized rate theory of radiation-induced conduction we developed a theoretical model which includes the effect of the applied electric field on the carrier generation yield (geminate recombination, Onsager effect). Comparison of experimental and theoretical curves allowed us to determine the values of the main conduction parameters, such as carrier mobility, recombination coefficient, trap densities, for the materials under investigation.

Foi estudada a enxertia de estireno induzida por radiação gama em filmes de poli(fluoreto de vinilideno) (PVDF) com espessura de 0,125 mm com doses entre 1 e 100 kGy em presença de soluções de estireno/N,Ndimetilformamida (DMF) (1:1, v/v) e estireno/tolueno (1:1, v/v) com taxa de dose de 5 kGy.h-1 por meio do método simultâneo de irradiação sob atmosfera de nitrogênio e em temperatura ambiente, usando raios gama de uma fonte de Co- 60. Depois de enxertados, os polímeros foram sulfonados em soluções de ácido clorossulfônico/1,2-dicloroetano (2 e 10 %). Os filmes foram caracterizados antes e depois de cada modificação com o cálculo do Grau de enxertia, (DOG), espectrometria no infravermelho (FT-IR), microscopia eletrônica de varredura (MEV), calorimetria exploratória diferencial (DSC) e termogravimetria (TG/DTG). Os resultados do grau de enxertia mostraram que a enxertia aumenta com o aumento da dose e varia enormemente de acordo com o solvente utilizado, com enxertias cerca de 20 vezes maiores quando do uso da DMF em relação ao do tolueno. Foi possível confirmar a enxertia do estireno por FT-IR graças ao aparecimento de novos picos característicos e por TG/DTG e DSC por meio das alterações do comportamento térmico dos materiais enxertados/sulfonados. Os materiais sulfonados ainda foram caracterizados por suas capacidades de troca iônica (IEC), que mostraram que tanto os aumentos do grau de enxertia quanto os da concentração do ácido clorossulfônico aumentam o IEC. Os resultados mostraram que é possível obter materiais com capacidades de troca iônica com possibilidade de aplicação como compósitos ionoméricos de metal-polímero. / Gamma-radiation-induced grafting of styrene into poly(vinylidene fluoride) (PVDF) films with 0.125 mm thickness at doses from 1 to 100 kGy in the presence of a styrene/N,N- dimethylformamide (DMF) solution (1:1, v/v) and styrene/toluene (1:1, v/v) at dose rate of 5 kGy h-1 was carried out by simultaneous method under nitrogen atmosphere at room temperature, using gamma rays from a Co-60. After grafting reactions, the polymer was then sulfonated in chlorosulfonic acid/1,2-dichloroethane (2 and 10%) for 3 hours. The films were characterized before and after modification by calculating the degree of grafting (DOG), infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and thermogravimetry (TG/DTG). DOG results show that grafting increases with dose, and varies enormously depending on the solvent used, with DOGs about 20 times greater in DMF than in toluene. It was possible to confirm the grafting of styrene by FT-IR due to the appearance of the new characteristic peaks and by the TG and DSC which exhibited changes in the thermal behavior of the grafted/sulfonated material. Sulfonated material was also characterized by ion exchange capacity (IEC) showed that both DOG and sulfonic acid concentration increase IEC values. Results showed that it is possible to obtain materials with ion exchange capacity of possible application as ionic polymer-metal composites.

La fibrose radio-induite est une complication sévère et tardive de la radiothérapie. Plusieurs études cliniques ont montré que la combinaison pentoxifylline-vitamine E est un traitement sûr et efficace contre la fibrose. Cependant, les mécanismes moléculaires de son efficacité restent inexplorés. Nous avons montré l’efficacité de la combinaison pentoxifylline-vitamine E dans l’entéropathie radique dans une faisabilité clinique. En parallèle, en utilisant un modèle unique, in vitro, de cellules musculaires lisses intestinales primaires isolées des personnes atteintes de l’entéropathie radique, nous avons montré une synergie entre la pentoxifylline et l’analogue hydrophile de vitamine E (trolox) qui permet à l’association d’inhiber l’expression de TGF-β1 au niveau de l’ARN messager et de la protéine. Cette action inhibitrice intervient au niveau transcriptionnel et conduit à une inhibition conséquente des cibles de la voie de signalisation TGF-β1/Smad (Col Iα1, FN1, PAI-1, CTGF), alors qu’elle semble sans effet sur la voie de signalisation Rho/ROCK. Pour la première fois, dans ces cellules issues de l’entéropathie radique, nous avons montré une surexpression de miR-210 ; microRNA induit par l’hypoxie. L’association pentoxifylline-trolox inverse la surexpression de miR-210 aussi bien dans les conditions normoxique que dans les conditions hypoxiques. L’implication de miR-210 dans l’entéropathie radique n’a pas été préalablement étudiée, néanmoins nous avons montré qu’un inhibiteur de miR-210 diminue l’expression de Col Iα1 dans ce modèle. L’effet anti-fibrosant exercé par l’association pentoxifylline-vitamine E est partiellement induit par l’inhibition de la cascade TGF-β1. L’inhibition de miR-210 est un deuxième mécanisme potentiel nécessitant d’autres investigations. Cette étude renforce les essais clinique antérieurs en montrant in vitro une synergie entre pentoxifylline et vitamine E et permettant de proposer cette association en première ligne thérapeutique dans la fibrose radio-induite. De plus, miR-210 est proposé comme une possible cible thérapeutique pour traiter la fibrose radio-induite. / Radiation-induced fibrosis is a serious late complication of radiotherapy. Pentoxifylline-vitamin E has proven effective and safe in clinical trials as treatment of fibrosis, while the molecular mechanism of its activity is yet unexplored. We showed efficacy of Pentoxifylline-vitamin E combination in radiation-induced enteropathy in a small clinical study. In parallel, using a unique in vitro model of primary smooth muscle cells isolated from intestinal samples isolated from humans with radiation enteropathy we showed that pentoxifylline and the hydrophilic analogous of vitamin E (trolox) synergize to inhibit TGF-β1 protein and mRNA expression. This inhibitory action is mediated at the transcriptional level and leads to subsequent inhibition of TGF-β1/Smad targets (Col Iα1, FN1, PAI-1, CTGF), while it has no effect on the Rho/Rock pathway. We have also demonstrated, for the first time, an overexpression of the hypoxia-induced microRNA miR-210 in the fibrotic cells. Pentoxifylline-trolox combination could reverse this miR-210 overexpression in normoxic and hypoxic conditions. While miR-210 has not been previously shown to be involved in radiation-induced enteropathy, we showed that miR-210 inhibitor could reduce mRNA expression of Col Iα1. The anti-fibrotic effect of combined pentoxifylline-vitamin E is at least in part mediated by inhibition of the TGF-β1 cascade. MiR-210 inhibition is another mechanism which needs further investigations. This study strengthens previous clinical data showing pentoxifylline-vitamin E synergy and supports its use as a first-line treatment of radiation-induced fibrosis. Also, it suggests miR-210 as a new potential therapeutic target for the treatment of this complication.

Foi estudada a enxertia de estireno induzida por radiação gama em filmes de poli(fluoreto de vinilideno) (PVDF) com espessura de 0,125 mm com doses entre 1 e 100 kGy em presença de soluções de estireno/N,Ndimetilformamida (DMF) (1:1, v/v) e estireno/tolueno (1:1, v/v) com taxa de dose de 5 kGy.h-1 por meio do método simultâneo de irradiação sob atmosfera de nitrogênio e em temperatura ambiente, usando raios gama de uma fonte de Co- 60. Depois de enxertados, os polímeros foram sulfonados em soluções de ácido clorossulfônico/1,2-dicloroetano (2 e 10 %). Os filmes foram caracterizados antes e depois de cada modificação com o cálculo do Grau de enxertia, (DOG), espectrometria no infravermelho (FT-IR), microscopia eletrônica de varredura (MEV), calorimetria exploratória diferencial (DSC) e termogravimetria (TG/DTG). Os resultados do grau de enxertia mostraram que a enxertia aumenta com o aumento da dose e varia enormemente de acordo com o solvente utilizado, com enxertias cerca de 20 vezes maiores quando do uso da DMF em relação ao do tolueno. Foi possível confirmar a enxertia do estireno por FT-IR graças ao aparecimento de novos picos característicos e por TG/DTG e DSC por meio das alterações do comportamento térmico dos materiais enxertados/sulfonados. Os materiais sulfonados ainda foram caracterizados por suas capacidades de troca iônica (IEC), que mostraram que tanto os aumentos do grau de enxertia quanto os da concentração do ácido clorossulfônico aumentam o IEC. Os resultados mostraram que é possível obter materiais com capacidades de troca iônica com possibilidade de aplicação como compósitos ionoméricos de metal-polímero. / Gamma-radiation-induced grafting of styrene into poly(vinylidene fluoride) (PVDF) films with 0.125 mm thickness at doses from 1 to 100 kGy in the presence of a styrene/N,N- dimethylformamide (DMF) solution (1:1, v/v) and styrene/toluene (1:1, v/v) at dose rate of 5 kGy h-1 was carried out by simultaneous method under nitrogen atmosphere at room temperature, using gamma rays from a Co-60. After grafting reactions, the polymer was then sulfonated in chlorosulfonic acid/1,2-dichloroethane (2 and 10%) for 3 hours. The films were characterized before and after modification by calculating the degree of grafting (DOG), infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and thermogravimetry (TG/DTG). DOG results show that grafting increases with dose, and varies enormously depending on the solvent used, with DOGs about 20 times greater in DMF than in toluene. It was possible to confirm the grafting of styrene by FT-IR due to the appearance of the new characteristic peaks and by the TG and DSC which exhibited changes in the thermal behavior of the grafted/sulfonated material. Sulfonated material was also characterized by ion exchange capacity (IEC) showed that both DOG and sulfonic acid concentration increase IEC values. Results showed that it is possible to obtain materials with ion exchange capacity of possible application as ionic polymer-metal composites.

Made available in DSpace on 2016-04-27T13:10:23Z (GMT). No. of bitstreams: 1 Monica Oliveira Bernardo.pdf: 3960791 bytes, checksum: cdb03a40c1d26631a8419067ae0e81eb (MD5) Previous issue date: 2013-12-17 / Radiological examinations have greatly increased, especially in children after craniocerebral trauma (CCT). Recent studies indicate a higher incidence of cancer and cataracts in children undergoing computed tomography (CT) compared to those without exposure. Some countries have promoted campaigns to avoid unnecessary CT and reducing the radiation dose. Objectives: 1. To assess whether the reduction of radiation dose in CT affect exam interpretation and diagnosis in children with CCT. 2. To stimulate discussion and implementation of measures to reduce the radiation dose received by children requiring health care and, also, stimulating awareness of pediatricians and families about the radiation dangers. Methods: We selected two groups of CT from children with CCT that underwent to CT performed in Philips 64 channels Multi Slice CT scanner at Unimed Hospital (Sorocaba-SP) from January to August 2012. We initially selected the 30 last CT performed with usual radiation dose in children. Then to the next CT for CCT we apply the protocols to reduce radiation load (approximately 50%) according to the guidelines of The Alliance for Radiation Safety in Pediatric Imaging. We reduced the kilovoltage (KV) to the maximum shown by tomography equipment and set a limit to the milliamperes per second (mAs) according to analysis of the tomography equipment after the completion of digitized radiography tomography, according to thickness and length of area of the patient being examined. We oriented the technical team to restrict the exam to the extent of the area requested by the doctor. The two CT series were presented to 19 pediatricians and 02 neurosurgeons from the Emergency Unit and to 7 radiologists from Unimed Hospital blind to the technical differences. The participants answered a specific questionnaire asking if they noticed any difference comparing the two series of exams; if they had any difficulty in making the diagnosis and taking the necessary conduct; if they need any training to exam the CTs and to assess the load of radiation and, finally, if they consider useful to implement a booklet for each child to register his/her radiological examinations. Results: Four participants noticed differences between the CT series and reported greater "noise" (image graininess) in those with reduced radiation load, had no difficulty in making a diagnosis and take the right conduct; most would like to have training and education on radioprotection and all agreed that the booklet to register and control radiological examinations would be useful for education and surveillance of parents and health professionals. Conclusion: This study showed that is possible to reduce the radiation dose in CT scans of children up to 50% without loss in the diagnosis accuracy; health professionals are motivated for continuing education and have attitudes to reduce the load of radiation. The direction of the hospital implemented the booklet to record radiological examinations. The campaign was publicized in the local media and on websites of Unimed Sorocaba and Unimed Brazil and has been well accepted by the hospital community and families / Os exames radiológicos têm aumentado muito, especialmente em crianças pós-trauma crânio-encefálico (TCE). Estudos recentes indicam maior incidência de câncer e catarata em crianças submetidas à tomografia quando comparadas àquelas sem exposição. Alguns países já promovem campanhas para evitar exames desnecessários e reduzir a dose de radiação. Objetivos: 1. Avaliar se a redução da dose de radiação em tomografias computadorizadas (TC) de crânio prejudica a interpretação do exame e o diagnóstico em crianças com TCE. 2. Desencadear no ambiente de trabalho a discussão e implementação de medidas capazes de reduzir a dose de radiação recebida por crianças que necessitem atenção à saúde estimulando também a conscientização dos pediatras e dos familiares. Métodos: Selecionamos dois grupos de TC de crianças com TCE, realizados em tomógrafo Philips Multi Slice de 64 canais no Hospital da Unimed de Sorocaba no período de janeiro a agosto de 2012. Inicialmente selecionamos as 30 últimas TC realizadas com dose de radiação habitual. A seguir, aos próximos exames, aplicamos os protocolos de redução da carga de radiação (aproximadamente 50%) segundo as diretrizes da The Alliance for Radiation Safety in Pediatric Imaging. Reduzimos a Quilovoltagem (KV) ao máximo demonstrado pelo equipamento de tomografia e estabelecemos um limite para a Miliamperagem por segundo (mAs) de acordo com a análise do equipamento de tomografia após a realização da radiografia digitalizada na tomografia, de acordo a espessura e o comprimento da área a ser analisada do paciente. Orientamos a equipe técnica para que restringisse a extensão do exame somente à região solicitada no pedido médico. As duas séries de exames foram apresentados a 19 pediatras, 2 neurocirurgiões da Unidade de Emergência e a 7 radiologistas do Hospital da Unimed Sorocaba que desconheciam as diferenças técnicas. Os participantes responderam por um questionário específico se encontraram diferenças entre os exames; se tiveram dificuldade em fazer o diagnóstico e tomar a conduta necessária; se sentiam necessidade de capacitação para avaliar os exames e a carga de radiação e se consideravam útil a implantação de uma caderneta individual de registro dos exames radiológicos. Resultados: Quatro participantes viram diferenças entre os exames e referiram maior "ruído" (granulação da imagem) naqueles com redução da carga de radiação; não tiveram dificuldade em realizar o diagnóstico e orientar a conduta; a maioria gostaria de ter capacitação e educação preventiva e todos concordam que a caderneta de controle de registro de exames radiológicos seria útil para a educação e vigilância dos pais e profissionais de saúde. Conclusão: O estudo evidenciou ser possível reduzir a dose de radiação em TC de crianças em até 50% sem prejuízo no diagnóstico e na conduta; os profissionais de saúde estão motivados a se capacitarem e a ter atitudes visando reduzir a carga de radiação. A direção do hospital implantou a caderneta de registro de exames radiológicos. A campanha foi divulgada na mídia local e nos sites da Unimed de Sorocaba e na Unimed do Brasil e tem sido bem aceita pela comunidade hospitalar e familiares

Diss. (sammanfattning) Stockholm : Karol. inst., 2003. / Härtill 5 uppsatser.

EDF souhaite utiliser la technologie de mesure de température répartie par capteur à fibre optique utilisant l’effet Raman pour réaliser des cartographies de température de certains composants de centrales nucléaires. Les conditions environnementales auxquelles le capteur à fibre optique est soumis sont particulièrement agressives (température de 350 °C et rayonnements gamma ionisants). Les rayonnements ionisants sont responsables de la création de défauts structurels au cœur de la fibre, qui atténuent sa transmission lumineuse, et dont les effets engendrent une erreur de mesure de température pouvant aller jusqu'à l’interruption totale de la mesure. La haute température, quant à elle, dégrade le revêtement protecteur de la fibre optique, ce qui la fragilise mécaniquement. Des irradiations gamma in situ sur des fibres optiques multimodes commerciales à revêtement or protégées par une gaine en acier inoxydable ont été réalisées, à l’aide de deux sources de rayonnements différentes, pour observer l'atténuation radio-induite du capteur à fibre optique en fonction du débit de dose et de la dose cumulée. Les effets du rayonnement à température ambiante, puis à haute température, ont été observés. Ce travail expérimental démontre que la haute température peut être maîtrisée grâce à une fibre à revêtement or, et que la haute température est bénéfique contre l’atténuation de la fibre engendrée par l’irradiation. La mise en œuvre de capteur de température à fibre optique en environnement sévère devient possible, ainsi que l’estimation des incertitudes sur la mesure associée. / EDF is working on Raman distributed temperature sensing using optical fiber sensors in order to map temperature of nuclear power plants big components. The sensor has to sustain harsh environmental conditions (temperatures up to 350 °C and gamma ionizing radiations). Ionizing radiations can create structural defects inside the fiber’s core, which attenuate the light transmission. This phenomenon can lead to temperature measurements errors until no measurement is possible. As for high temperature, it can affect the fiber coating, which mitigate the fiber mechanical resistance.Gamma rays in situ irradiations have been carried out over commercial off-the-shelf multimode gold coated fibers protected with a stainless steel metal tubing, with two different radiation sources, in order to observe radiation-induced attenuation over dose rate or cumulated dose. Effects of gamma rays over gold coated optical fiber sensors have been observed at both room anhigh temperature.This experimental work enlightens that high temperature can be controlled with gold coated fibers, and that the radiation-induced attenuation downsides can efficiently be balanced with high temperature. Implementation of a Raman distributed temperature optical fiber sensor in such harsh environments becomes possible, as well as the associated estimation of measurement uncertainty.