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Effect of BAS125 10W on late spring/early summer alfalfa growth, 1998Rethwisch, Michael D., Kruse, Michael D. 10 1900 (has links)
Four rates of BAS125 were evaluated to document rate effects on reduction in stem elongation and associated increase in alfalfa leaves during the peak period of summer growth. Increasing rates of BAS125 resulted in greater inhibition of alfalfa stem elongation, as the highest rate (0.1875 lbs. a.i./acre) had stems 27-37% shorter than the untreated check through 12 days post treatment, and 20% shorter thereafter. Alfalfa weights (both stem and leaf) were also reduced by BAS125 treatments through 12 days after treatment. Higher rates resulted in lighter leaves. Significantly more trifoliate leaves were noted at the higher rates of BAS125 at 19 days after treatment, as was increased leaf and stem weights than untreated check with most noted at the 0.125 rate. Stem diameters were not significantly different, although untreated stems were wider. Numerically more open flowers were documented with usage of BAS125 at 19 days post treatment, but fewer floral racemes/stem were associated with higher rates at 27 days post treatment. No differences existed for alfalfa plant height measurements in regrowth following harvest, indicating that application of BAS125 are only effective on forage harvest to which they were applied and have no subsequent residual effect.
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Effect of BAS125 on low desert alfalfa growth and quality during the August production periodRethwisch, Michael D., Kruse, Michael D., Kallenbach, Robert, Goad, Marcus 10 1900 (has links)
Alfalfa was treated with five rates of BAS125 on August 5, 1997. Data were obtained to determine rate effects on stem regrowth (both height and width), trifoliate leaves, and yield. Variation in plot area affected some results. No statistical differences were noted for quality classification or yields, although increased protein levels were noted from BAS125 treatments. Increasing BAS125 rates resulted in significant decreases in plant height and significant increases in numbers of trifoliate leaves at nodes 1-6. Differences in stem diameters were also noted, with stem width affected by rate of BAS125 at lower portions of stem. Differences in stem diameters of upper parts of stems were primarily associated with plant height reductions as a function of BAS125 rate.
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Structural biology of Vibrio cholerae pathogenicity factorsSheikh, Md. Arif January 2009 (has links)
The World Health Organization (WHO) states that 30,000 children under the age of five die each day worldwide. Around a quarter of these die from diarrheal disease caused by microbial infection. In addition to this high mortality rate, there are data emerging on the morbidity effects of diarrheal disease, for example a few episodes of diarrhea in the first two years of life can remove 10 IQ points and lead to growth deficiency. Vibrio cholerae, the causative agent of the diarrheal disease cholera, is a serious problem in third world countries, where sanitary and hygiene infrastructure is very poor, and claims several thousand lives every year. In order to better understand the pathogenicity regulation in V. cholerae, structural and functional investigations of a hypothetical protein family present in pathogenicity islands and a transcriptional regulator protein for DNA-binding were investigated. Two adjacent genes, vc1804 and vc1805, encode hypothetical proteins within the Vibrio pathogenicity island-2 (VPI-2) of Vibrio cholerae, and are part of a cluster of genes only present in pathogenic strains of the bacterium. Paralogous adjacent genes, vc0508 and vc0509, are also present within a second pathogenicity island, the Vibrio seventh pandemic island-2 (VSP-2), of V. cholerae O1 El Tor and O139 serogroup isolates. Sequence similarity suggests that the VC0508, VC0509, VC1804 and VC1805 proteins will share a similar fold. The crystal structures of VC0508, VC0509 and VC1805 have been determined to a resolution of 1.9, 2.4 and 2.1 Å, respectively. Several recombinant constructs of vc1804 were made, but no soluble proteins were expressed. This hypothetical protein family reveals structural homology to human mitochondrial protein p32. Human p32 is a promiscuous protein known to bind to a variety of partners including the globular head component of C1q. We have shown that VC1805 binds to C1q. One possibility is that VC1805 is involved in adherence of the bacterium to membrane-bound C1q in the gut. To explore the roles of VC0508, VC0509, VC1804 and VC1805 in vivo, gene knockout and animal model studies of those proteins are underway. The ferric uptake regulator (Fur), a metal-dependent DNA-binding protein, acts as both a repressor and activator of numerous genes involved in maintaining iron homeostasis in bacteria. It has also been demonstrated in Vibrio cholerae that Fur plays an additional role in pathogenesis, and this opens up the potential of Fur as a drug target for cholera. The first crystal structure of a Fur protein, from Pseudomonas aeruginosa, revealed a dimeric molecule with each monomer containing a dimerization domain, a helical DNA-binding domain and two metal binding sites: Zn1 is proposed to be a regulatory Fe-binding site, and Zn2 is proposed to be a structural Zn-binding site. Here we present the crystal structure of V. cholerae Fur (VcFur) that reveals a very different orientation of the DNA-binding domains. Accompanying these structural changes are alterations in the amino acids coordinating the zinc at the Zn2 site, and this lends support to this being the site regulated by iron. There is no evidence of metal binding to the cysteines that are conserved in many Fur homologues, including the much-studied E. coli Fur. An analysis of the metal binding properties shows that like other Fur proteins, VcFur can be activated by a range of divalent metals. EPR spectroscopy measurements of the movements of the DNA-binding domain, in the presence of DNA and different metals, are underway.
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Structure-Function Studies on Aspartate Transcarbamoylase and Regulation of Pyrimidine Biosynthesis by a Positive Activator Protein, PyrR in Pseudomonas putidaKumar, Alan P. 12 1900 (has links)
The regulation of pyrimidine biosynthesis was studied in Pseudomonas putida. The biosynthetic and salvage pathways provide pyrimidine nucleotides for RNA, DNA, cell membrane and cell wall biosynthesis. Pyrimidine metabolism is intensely studied because many of its enzymes are targets for chemotheraphy. Four aspects of pyrimidine regulation are described in this dissertation. Chapter I compares the salvage pathways of Escherichia coli and P. putida. Surprisingly, P. putida lacks several salvage enzymes including nucleoside kinases, uridine phosphorylase and cytidine deaminase. Without a functional nucleoside kinase, it was impossible to feed exogenous uridine to P. putida. To obviate this problem, uridine kinase was transferred to P. putida from E. coli and shown to function in this heterologous host. Chapter II details the enzymology of Pseudomonas aspartate transcarbamoylase (ATCase), its allosteric regulation and how it is assembled. The E. coli ATCase is a dodecamer of two different polypeptides, encoded by pyrBI. Six regulatory (PyrI) and six catalytic (PyrB) polypeptides assemble from two preformed trimers (B3) and three preformed regulatory dimers (I2) in the conserved 2B3:3I2 molecular structure. The Pseudomonas ATCase also assembles from two different polypeptides encoded by pyrBC'. However, a PyrB polypeptide combines with a PyrC. polypeptide to form a PyrB:PyrC. protomer; six of these assemble into a dodecamer of structure 2B3:3C'2. pyrC' encodes an inactive dihydroorotase with pyrB and pyrC' overlapping by 4 bp. Chapter III explores how catabolite repression affects pyrimidine metabolism. The global catabolite repression control protein, Crc, has been shown to affect pyrimidine metabolism in a number of ways. This includes orotate transport for use as pyrimidine, carbon and nitrogen sources. Orotate is important because it interacts with PyrR in repressing the pyr genes. Chapter IV describes PyrR, the positive activator of the pyrimidine pathway. As with other positive activator proteins, when pyrimidine nucleotides are depleted, PyrR binds to DNA thereby enhancing expression of pyrD, pyrE and pyrF genes. When pyrimidine nucleotides are in excess, the PyrR apoprotein binds to orotate, its co-repressor, to shut down all the pyrimidine genes. Like many positive activators, PyrR is subject to autoregulation and has catalytic activity for uracil phosphoribosyltransferase inducible by orotate.
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Identification and characterization of Clostridium sordellii toxin gene regulatorSirigi Reddy, Apoorva Reddy January 1900 (has links)
Master of Science / Division of Biology / Revathi Govind / Toxigenic Clostridium sordellii causes uncommon but highly lethal infections in humans and animals. Recently, an increased incidence of C. sordellii infections has been reported in women undergoing obstetric interventions. Pathogenic strains of C. sordellii produce numerous virulence factors, including sordellilysin, phospholipase, neuraminidase, and two large clostridial glucosylating toxins, TcsL and TcsH. Recent studies have demonstrated that TcsL toxin is an essential virulence factor for the pathogenicity of C. sordellii. In this study, we identified and characterized TcsR as the toxin gene (tcsL) regulator in C. sordellii. High-throughput sequencing of two C. sordellii strains revealed that tcsR lies within a genomic region that encodes TcsL, TcsH, and TcsE, a putative holin. By using ClosTron technology, we inactivated the tcsR gene in strain ATCC 9714. Toxin production and tcsL transcription were decreased in the tcsR mutant strain. However, the complemented tcsR mutant produced large amounts of toxins, similar to the parental strain. Expression of the Clostridium difficile toxin gene regulator tcdR also restored toxin production to the C. sordellii tcsR mutant, showing that these sigma factors are functionally interchangeable.
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Mitigation of random and deterministic noise in mixed signal systems with examples in frequency synthesizer systemsBurress, Thomas Weston January 1900 (has links)
Master of Science / Department of Electrical and Computer Engineering / William B. Kuhn / RF frequency synthesizer systems are prevalent in today’s electronics. In a synthesizer there is a sensitive analog oscillator that may be affected by two different types of noise.
The first is random noise injection from active devices. This results in phase noise in the synthesizer’s spectrum. The second noise source is deterministic. A digital frequency divider with high-amplitude switching is an example of such a deterministic source. This noise enters the system through various forms of electric or magnetic field coupling and manifests itself as spurs or pulling. Both forms of noise can adversely affect system performance.
We will first summarize methods for reducing noise. These already known steps have to do with layout techniques, device geometry, and general synthesizer topologies. Then we will show ways to isolate noisy interfering circuits from the sensitive analog systems. Finally, we present some considerations for reducing the effects of random noise.
A power supply filter can improve the effects of deterministic noise such as undesired signals on the supply line. We show several ways to improve the rejection of high frequency supply noise (characterized by the power supply rejection ratio or PSRR) through the design of a voltage regulator. The emphasis is on new techniques for obtaining good PSRR at S-band frequencies and above.
To validate the techniques, we designed a regulator in Peregrine Semiconductor’s .25µm ULTRA CMOS Silicon on Sapphire process. It produces a 2.5V output with an input ranging from 2.6V to 5V and has a maximum current sourcing of 70mA. The regulator’s low drop out performance is 60mV with no load and it achieves a power supply ripple reduction of 29.8 dB at 500 MHz.
To address random noise in synthesizers, the thesis provides preliminary investigation of an oscillator topology change that has been proposed in the literature. This proposed change reduces the phase noise of the oscillator within the overall system. A differential cross-coupled design is the usual topology of choice, but it is not optimal for noise performance. We investigate current noise injection in the traditional design and present an updated design that uses a differential Colpitts oscillator as an alternative to classic cross-coupled designs.
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Oncolytic Therapy with Vaccinia Virus GLV-1h68 - Comparative Microarray Analysis of Infected Xenografts and Human Tumor Cell Lines - / Onkolytische Therapy mit Vaccinia Virus GLV-1h68 - Vergleichende Mikroarray Analyse von infizierten Xenografts und humanen Tumorzelllinien -Worschech, Andrea January 2010 (has links) (PDF)
Aim of this thesis was to study the contribution of the hosts immune system during tumor regression. A wild-type rejection model was studied in which tumor regression is mediated through an adaptive, T cell host response (Research article 1). Additionally, the relationship between VACV infection and cancer rejection was assessed by applying organism-specific microarray platforms to infected and non-infected xenografts. It could be shown that tumor rejection in this nude mouse model was orchestrated solely by the hosts innate immune system without help of the adaptive immunity. In a third study the inflammatory baseline status of 75 human cancer cell lines was tested in vitro which was correlated with the susceptibility to VACV and Adenovirus 5 (Ad5) replication of the respective cell line (Manuscript for Research article 3). Although xenografts by themselves lack the ability to signal danger and do not provide sufficient proinflammatory signals to induce acute inflammation, the presence of viral replication in the oncolytic xenograft model provides the "tissue-specific trigger" that activates the immune response and in concordance with the hypothesis, the ICR is activated when chronic inflammation is switched into an acute one. Thus, in conditions in which a switch from a chronic to an acute inflammatory process can be induced by other factors like the immune-stimulation induced by the presence of a virus in the target tissue, adaptive immune responses may not be necessary and immune-mediated rejection can occur without the assistance of T or B cells. However, in the regression study using neu expressing MMC in absence of a stimulus such as a virus and infected cancer cells thereafter, adaptive immunity is needed to provoke the switch into an acute inflammation and initiate tissue rejection. Taken together, this work is supportive of the hypothesis that the mechanisms prompting TSD differ among immune pathologies but the effect phase converges and central molecules can be detected over and over every time TSD occurs. It could be shown that in presence of a trigger such as infection with VACV and functional danger signaling pathways of the infected tumor cells, innate immunity is sufficient to orchestrate rejection of manifested tumors. / Ziel dieser Arbeit war, die Beteiligung des Wirts-eigenen Immunsystems bei der Tumoregression zu analysieren. Mittels eines Wildtyp-Regressionsmodells, wurde der Anteil des adaptiven Immunsystems studiert (Research-Artikel 1). Mit Hilfe von Organismus-spezifischen Mikroarrays und Genexpressionsanalysen konnte in einem Nacktmausmodell gezeigt werden, dass erfolgreiche, durch onkolytische VACV-vermittelte Tumortherapie auch ohne Beteiligung des adaptiven Immunsystems möglich ist (Research Artikel 2). In einer dritten Studie wurden 75 humane Tumorzelllinien auf ihren intrinsischen Entzündungsstatus hin getestet und bezüglich eines Zusammenhanges von diesem mit der Replikationsfähigkeit von VACV und Adenovirus 5 (Ad5) analysiert (Manuskript für den Research-Artikel 3). Obwohl Xenografts allein kein ausreichendes „Gefahrsignal“ geben und durch das Fehlen einer pro-inflammatorischen Stimulierung keine akute Entzündung verursachen können, ist die Infektion mit onkolytischem VACV ausreichend, um den Gewebe-spezifischen „Trigger“ darzustellen. In diesem Fall wird die Immunantwort aktiviert und nach der Hypothese des „Immunologic Constant of Rejection“ (ICR) geschieht dies, wenn eine chronische in eine akute Inflammation verändert wird. In dem beschriebenen onkolytischen Regressionsmodell ist die Präsenz des Virus ausreichend, um das Immunsystem zu aktivieren, d.h. die chronische Entzündung im Tumor in eine akute umzuwandeln. Dabei ist die adaptive Immunität mit T- und B-Zell-Aktivierung nicht notwendig für die Rückbildung des Tumors. In Abwesenheit eines solchen Stimulus, wie in der ersten Studie mit neu-exprimierenden MMCs, wird die Spezifität der adaptiven Immunantwort benötigt, um die akute Inflammation anzustoßen und die Tumorregression voranzutreiben. Zusammengefasst unterstützt diese Arbeit die Hypothese, dass die Mechanismen, die zu „tissue specific destruction“ (TSD) führen, in verschiedenen immunologischen Erkrankungen zwar divergieren, der Effektor-Mechanismus aber stets der Gleiche ist. Es zeigte sich, dass in Anwesenheit eines „triggers“, wie z.B. der VACV-Infektion und intakten „danger signaling pathways“ der Tumorzellen, die angeborene Immunität allein ausreicht, um die Tumorrückbildung zu vermitteln.
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Estudos cristalográficos da proteína ElrR, regulador transcricional do fator de virulência ElrA de Enterococcus faecalis, e indícios de sua interação com a região de ligação ao DNA / Crystallographic studies of the protein ElrR, a transcriptional regulator of the Enterococcus faecalis virulence factor ElrA, and indications of its interaction with DNA fragmentGroote, Michel Conrad Robert De 21 November 2017 (has links)
A ampliação do conhecimento sobre as formas de comunicação, controle e regulação existentes em bactérias traz luz aos avanços no combate das infecções hospitalares que são responsáveis por inúmeros prejuízos relacionados à saúde pública em todo planeta. DUMOULIN et al (2013), descreveram o regulador transcricional (RT) ElrR, que regula positivamente a transcrição do gene elrA, um fator de virulência de Enterococcus faecalis. ElrA apresenta grande similaridade com as internalinas de Listeria monocytogenes, que facilitam a invasão da bactéria ao hospedeiro. ElrR é considerada como pertencente à família Rgg-like de RT exclusivo de bactérias Gram positivas. Por vários motivos a família Rgg foi inserida à superfamília RNPP, gerando a superfamília RRNPP de RT. Os RRNPP fazem parte de um sistema de regulação por quorum sensing (QS), um sistema de comunicação célula-célula dependente de densidade celular, com função associada na ativação ou inibição da expressão de proteínas relacionadas, dentre outros, à virulência, formação de biofilme e esporulação. Para a melhor compreensão do mecanismo de como ocorre a ativação da transcrição do fator de virulência ElrA, este trabalho apresenta resultados de expressão heteróloga em E. coli e purificação das proteínas ElrR e ElrA, bem como resultados de experimentos biofísicos que caracterizam algumas propriedades estruturais e biológicas destas proteínas. Utilizando técnicas de cromatografia, espectroscopia de dicroísmo circular (CD), anisotropia de fluorescência, espalhamento dinâmico de luz (DLS), cristalografia de raios X e ressonância plasmônica de superfície (SPR), foi possível a obtenção da estrutura tridimensional de ElrR e de indícios da interação com uma região de 25bp do DNA. Realizou-se ainda, em colaboração com Dra. Pascale Serror, a tentativa de obtenção da molécula autoindutora (AI) de ElrR. São apresentados primeiros resultados da obtenção heteróloga de ElrA, sua purificação e cristalização, com importantes características que permitirão a continuação da investigação deste fator de virulência. ElrR é composta somente por alfa-hélices e apresenta-se dimérico em solução. Apesar da similaridade estrutural dos RRNPP, a identidade da sequência entre ElrR e os outros membros é extremamente baixa, o que motivou a resolução das fases cristalográficas experimentalmente. A estrutura de ElrR apresenta-se similar às homólogas, porém, com maior interface de interação entre os protômeros, que formam o dímero. O sítio de ligação do AI, em ElrR, apresenta-se mais amplo, com cavidade maior que as demais estruturas estudadas, conservando vários dos resíduos apresentados nos homólogos que realizam a estabilização do AI. Os altos fatores de temperatura dos cristais de ElrR, adicionado a anisotropia dos átomos, de uma das estruturas obtidas, apresenta a grande flexibilidade desse RT. Os indícios de interação entre ElrR e DNA aqui apresentados, obtidos por SPR e anisotropia de fluorescência, apresentam que ElrR liga especificamente ao fragmento proposto do DNA, ainda na ausência do AI. A não cristalização do complexo (ElrR-DNA), adicionada a alta flexibilidade apresentada na estrutura e a instabilidade observada na ligação ao DNA (por SPR) apontam para a obrigatoriedade da molécula de regulação (AI) para que o complexo ElrR-DNA seja estável. / The enhancing of the knowledge about communication, control and regulation in bacteria bring possibilities on the advance of hospital-acquired infections control responsible for various prejudices related to public health worldwide. DUMOULIN, et al (2013) described ElrR, a transcriptional regulator (TR), that positively regulates transcription of the elrA gene, which codifies a virulence factor of Enterococcus faecalis. ElrA shows high similarity with Listeria monocytogeneses internalins, which facilitates host invasion by these bacteria. ElrR are considered belonging to Rgg-like TR family exclusive of Gram positive bacteria. Several reasons include the Rgg family into the RNPP superfamily, generating the RRNPP superfamily of TR. The RRNPP are controlled by a quorum sensing (QS) regulation system, a cell-cell communication system based on cellular density that activates or inhibits the expression of proteins related with virulence, biofilm formation, sporulation, and others. For a better understanding of the transcription activation mechanism of ElrA, this work shows ElrR and ElrA heterologous expression in E. coli and purification of these proteins, as well as biophysics assays to characterize some structural and biological features of both proteins. Using chromatography, circular dichroism (CD), fluorescence anisotropy, dynamic light scattering (DLS), X-ray crystallography and surface plasmon resonance (SPR) technics, it was possible to obtain the tridimensional structure of ElrR, and evidences of ElrR-DNA complex formation, confirming DNA interaction site of ElrR with a 25 bp fragment. In collaboration with Dr. Pascale Serror, we attempted to achieve the ElrR auto-induction (AI) molecule. Also, results of the heterologous obtainment of ElrA are presented, as well as ElrA purification and crystallization, presenting important characteristics which will allow the further investigation of this virulence factor in near future. ElrR is composed by alpha-helices presenting dimeric fold in solution. Despite the similarity between the RRNPP members, the low identity of ElrR to the other members motivates the experimental crystallographic phases solution. ElrR structure is very similar to the homologous structures, presenting a higher interface between the protomers that compose the dimer. Its AI binding site is wider than the other structures studied, conserving several amino acid residues presented at the homologous proteins, that stabilizes the AI molecule. High temperature factors of the amino acid residues showed in all the obtained ElrR crystallographic structures plus the anisotropy of the atoms in one of those structures show the high flexibility of this TR. The evidence of the ElrR-DNA complex presented in this study, obtained by SPR and fluorescence anisotropy, indicates that ElrR binds at the proposed DNA site even in the absence of the AI molecule. The failure to obtain the ElrR-DNA complex crystals added to the high flexibility presented at some places of the structure and the observed instability at the formed complex (observed at SPR) suggest the mandatory need of the AI molecule to create a stable ElrR-DNA complex.
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Efeito do anelamento e de doses de ácido giberélico na frutificação das uvas 'Niagara Rosada' e 'Vênus' nas regiões noroeste e da alta paulista do Estado de São Paulo. / Effect of girdling and gibberellic acid on the fruit set of 'Niagara Rosada' and 'Venus' grapevines growing in the northwest area of São Paulo state.Cáto, Stella Consorte 29 May 2002 (has links)
O presente trabalho foi desenvolvido com o objetivo de avaliar o efeito do ácido giberélico em doses crescentes, isoladamente ou em conjunto com o anelamento de ramos e/ou com o desponte de cachos sobre as características dos cachos, bagos e engaços das cultivares de uvas de mesa Niagara Rosada e Vênus. Foram realizados dois experimentos para cada cultivar nas regiões noroeste e da alta paulista do Estado de São Paulo, e os delineamentos estatísticos utilizados foram em blocos ao acaso, com cinco repetições sendo quatorze e dezoito tratamentos para 'Niagara Rosada' e vinte e oito tratamentos para cada experimento com 'Vênus'. Para todos os experimentos, o ácido giberélico nas doses de 0; 10; 22,5; 35; 47,5; 60 e 72,5ppm, fo i aplicado quinze dias após o pleno florescimento, através da imersão total dos cachos, com exceção de um experimento com 'Niagara Rosada', no qual foi acrescido mais duas doses de 85 e 97,5ppm. O anelamento nos ramos produtivos foi realizado também nesta mesma época. No primeiro experimento com 'Niagara Rosada' detectou-se que não houve influência do anelamento de ramos sobre a massa e o tamanho médio dos bagos. Também, a dose de 35ppm de ácido giberélico sendo aplicada isoladamente originou incrementos na massa e tamanho médio dos cachos e bagos. Esta mesma dose quando aplicada conjuntamente com o anelamento de ramos proporcionou incrementos no teor de sólidos solúveis totais do mosto. No segundo experimento com 'Niagara Rosada', maiores incrementos no tamanho e massa dos bagos, assim como no diâmetro médio dos pedicelos foram obtidos com a aplicação de 60ppm de ácido giberélico conjuntamente com o anelamento de ramos. Já, os maiores incrementos na massa e na largura dos cachos e no número médio de bagos por cacho foram obtidos com a dose de 35ppm aplicada conjuntamente com a prática do anelamento. Já, com a cultivar Vênus no ciclo vegetativo de 1999 verificou-se que quanto maior a dose de ácido giberélico, maior foi o comprimento, a largura e a massa dos bagos. Também, o anelamento realizado isoladamente ou conjuntamente com o desponte de cachos proporcionou o aumento destas mesmas características. No experimento com a cultivar 'Vênus' no ciclo vegetativo de 2000 notou-se que quanto maior a dose de ácido giberélico, maior foi o comprimento e a largura dos cachos e engaços. A dose de 35ppm de ácido giberélico, aplicada conjuntamente com o anelamento de ramos isoladamente ou com o desponte de cachos, proporcionou as maiores massas de cachos e engaços e o maior número médio de bagos por cacho. Maiores bagos foram obtidos com a aplicação de 60ppm de ácido giberélico realizada conjuntamente com o anelamento de ramos isoladamente ou em conjunto com o desponte de cachos. A maior massa de bagos foi obtida com a aplicação isolada de 47,5ppm de ácido giberélico. Também, obteve-se menores teores de sólidos solúveis totais com doses maiores do regulador vegetal aplicadas conjuntamente com a técnica de incisão anelar. / The present work was developed to evaluate the effect on the cluster, berries and raquis characteristics of Niagara Rosada and Venus table grapes of increasing doses of gibberellic acid associated or not with branch girdling and/or cluster blunt. Two experiments, for each cultivar, were accomplished in the northwest area of São Paulo State, Brazil, in a randomized block design, with five replications, being fourteen and eighteen treatments for 'Niagara Rosada' and twenty-eight treatments for 'Venus'. The gibberellic acid in the doses of 0; 10; 22,5; 35; 47,5; 60 and 72,5ppm, was applied, through the total immersion of the clusters, fifteen days after the full flowering, except for the second experiment with 'Niagara Rosada', in which was added up two more doses of 85 and 97,5ppm. The girdling in the productive branches was also accomplished in this same time. In the first experiment with 'Niagara Rosada' no effect of girdling was detected on the berry mass and size. Also, gibberellic acid (35ppm) alone increased cluster and berry mass and size. This same dose when associated with girdling provided increase in the soluble solids. In the second experiment with 'Niagara Rosada', larger increments on berry size and mass, as well as, pedicels diameter were obtained with gibberellic acid (60ppm) associated with branch girdling. The largest increments on cluster mass and width and on the berry number per cluster, were obtained applying gibberellic acid (35ppm) associated with girdling. In the first experiment with 'Venus' seedless table grape in 1999 it was detected that the higher the concentrations of gibberellic acid, the larger were berry length, width and the mass. Also, girdling associated or not with cluster blunt increased these same characteristics. In the second experiment with 'Venus' cultivar it was noticed that the higher the concentrations of gibberellic acid, the larger were cluster and raquis length and width. Gibberellic acid (35ppm) associated with girdling only or with cluster blunt provided the largest increase on cluster and raquis masses and the largest berry number per cluster. Larger berries were obtained with gibberellic acid (60ppm) associated with girdling only or with cluster blunt. The largest berry mass was obtained with gibberellic acid (47,5ppm). Also, it was detected a decrease in soluble solids with higher concentrations of the vegetable regulator associated with girdling.
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Espaçamentos ultra-adensado, adensado e convencional com densidade populacional variável em algodoeiro. / Ultra-narrow row, narrow row and conventional row with cotton variable density plant population.Silva, Ariana Vieira 17 January 2003 (has links)
No presente estudo foi avaliado a densidade populacional, por espaçamentos ultra-adensado, adensado e convencional entre fileiras e número de plantas por metro linear, através do crescimento, desenvolvimento e produtividade da cultivar de algodão IAC 23. O experimento foi conduzido na área experimental da ESALQ/USP, município de Piracicaba, Estado de São Paulo, no ano agrícola de 2001/02. O delineamento experimental foi o de blocos ao acaso em esquema fatorial, com 4 repetições, sendo três espaçamentos entre linhas (0,38, 0,76 e 0,95 m) e quatro densidades de plantas por metro linear (5, 8, 11 e 14). O controle da altura das plantas foi realizado através de regulador de crescimento. Em cada parcela experimental foram marcadas seis plantas ao acaso e analisados os seguintes parâmetros: estádios fenológicos, altura média das plantas, altura de inserção do 1º ramo frutífero, diâmetro do caule, número de ramos vegetativos e frutíferos, número de internódios e o número de capulhos por planta. Em duas plantas foram avaliados o índice de área foliar e a massa seca da parte aérea. Na área útil de cada parcela foi determinada a produção de algodão em caroço, a precocidade de colheita e em amostras de 20 capulhos por parcela foram realizadas as análises dos caracteres agronômicos de laboratório (massa média de uma capulho, massa de 100 sementes e porcentagem de fibra) e das características tecnológicas da fibra (comprimento da fibra, uniformidade de comprimento, tenacidade, micronaire e maturidade). Pelos resultados obtidos concluí-se que as condições ambientais foram favoráveis para o crescimento e desenvolvimento da cultivar IAC 23, completando o ciclo em 161 dias; quanto menor o espaçamento e maior a densidade de plantas na linha, maior o IAF durante o ciclo, menor o diâmetro do caule pelo estiolamento das plantas e menor o número de capuhos por planta; quanto maior o espaçamento e menor a densidade de plantas na linha, maior a massa seca da parte aérea e a altura média das plantas durante o ciclo; o índice de colheita é maior conforme o aumento do espaçamento enquanto que não altera com a densidade de plantas; maior densidade de plantas na linha eleva a altura de inserção do 1º ramo frutífero; independente do espaçamento e da densidade, o número de ramos vegetativos não é alterado enquanto que o número de ramos frutíferos e o número de internódios diminui com o aumento da população de plantas por área; a produção de algodão em caroço foi 12% e 8,4% superior nos espaçamentos ultra-adensado e adensado, respectivamente, comparado ao convencional; no espaçamento ultra-adensado na menor densidade de plantas na linha, a produção de algodão em caroço foi 29,2% e 22,3% superior, respectivamente, às produções dos espaçamentos adensado e convencional na mesma densidade de plantas na linha; a precocidade de colheita praticamente não é alterada na cultivar IAC 23, sobretudo com o aumento da população de plantas por área; os caracteres agronômicos de laboratório e as características tecnológicas da fibra não foram alteradas nas populações de plantas estudadas. / The purpose of this work was to study plant population density, by spacing between rows and plants per linear meter for the IAC 23 cotton cultivar under ultra-narrow row, narrow row or conventional row by analyzing plant growth, development and yield. The experiment was established in the experimental field of ESALQ/USP, Piracicaba, São Paulo, during the 2001/2002 season, in a randomized block design with four replications. Three rows (0,38, 0,76 and 0,95 m between lines) and four plant densities (5, 8, 11 and 14 plants per linear meter) were used. Plant height was controlled by growth regulator. Six plants per plot were labeled and analyzed for the phenological phase, average plant height, insertion height of the first fruit branch, stem diameter, number of vegetative and fruit branches, number of internodes and open bolls per plant. Two plants were analyzed per plot for the leaf area index (LAI) and plant top dry mass. Cotton yield and harvest early were determined considering the effective plot area. Samples of 20 open bolls randomized chosen per plot were analyzed for agronomical lab characteristics (average open boll mass, mass of 100 seeds and fiber percentage) and fiber technological characteristics (length, length uniformity, strength, micronaire and maturity). Results showed that the environmental conditions favored growth and development of the IAC 23 cultivar, which completed its lifecycle in 161 days; smaller row and higher plant density resulted in greater LAI, smaller stem diameter due to estiolation and fewer open bolls per plant; larger row and lower plant density promoted greater plant top dry mass and average plant height; the harvest index increased with row, although it was not altered by plant density; higher plant density in rows increased the insertion height of the first fruit branch; the number of vegetative branches was not influenced by plant row and density, while the number of fruit branches and internodes decreased with level of plant population per area; cotton yield was 12% and 8,4% higher for the ultra-narrow row and narrow row, respectively, when compared with the conventional row; cotton yield for the ultra-narrow row with the lowest plant density in rows was 29,2% and 22,3% higher than the yields for the narrow row and conventional row, respectively, considering the same plant density; harvest early of the IAC 23 cultivar varied with the increase in plant population per area; agronomical lab characteristics and fiber technological characteristics did not vary in the plant populations studied.
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