A Internet consolidou as redes IP como uma infra-estrutura de transporte de dados barata e versátil, fato que justifica o crescente interesse nela como infraestrutura de transporte para outros serviços, como telefonia e TV. Esta convergência de serviços tem base no amadurecimento das tecnologias de redes de transmissão de dados, que proporcionou o aumento da velocidade de conexão residencial, tornando cada vez mais viável o uso de aplicações multimídia, inclusive em tempo real. Desta demanda de serviços convergentes, nasceu a digitalização da TV e, posteriormente, a possibilidade de transmissão pelas redes IP, conhecida como IPTV. Este trabalho analisa o cenário atual de IPTV para distribuição de conteúdo multimídia em redes de acesso privadas. Parte-se da necessidade de mecanismos de segurança para distribuição e controle do consumo das mídias digitais no sistema. O trabalho analisa, portanto, mecanismos para descrição inequívoca dos direitos que os usuários têm sobre o conteúdo adquirido, bem como a utilização de tecnologias para a transmissão do conteúdo de forma segura. O trabalho visa contribuir para a comunidade científica a partir da utilização de padrões abertos na especificação de um elemento de distribuição de vídeo em uma plataforma IPTV. Este elemento se baseia em políticas de uso da linguagem de expressão de direitos do MPEG-21 para reforçar os direitos de autoria e distribuição do conteúdo durante seu manuseio em toda a cadeia de negócios. / Internet consolidated IP networks as a versatile and accessible data transport infrastructure, thus justifying the growing interest on it as a transport network for several services, such as telephony and TV. The convergence leverages on the maturity of network technologies, responsible for the increasing availability of bandwidth for residential access, making the use of multimedia applications, including interactive ones, possible. From the demand for convergence, Digital TV has born, and later on, its transmission through IP networks, know as IPTV. This work analyzes current IPTV scenario for multimedia content distribution in the scope of private networks. It is based on the need for security mechanisms for digital content delivering and control of consumption in the system. Therefore it analyzes mechanisms for rights description, as well as security transmission of content. This work\'s contribution stems from the reutilization of open standards in the specification of a distribution broker in the IPTV environment. Such broker is based on the MPEG-21 digital rights description to enforce copyright of digital content during handling in the entire value chain.

A Internet consolidou as redes IP como uma infra-estrutura de transporte de dados barata e versátil, fato que justifica o crescente interesse nela como infraestrutura de transporte para outros serviços, como telefonia e TV. Esta convergência de serviços tem base no amadurecimento das tecnologias de redes de transmissão de dados, que proporcionou o aumento da velocidade de conexão residencial, tornando cada vez mais viável o uso de aplicações multimídia, inclusive em tempo real. Desta demanda de serviços convergentes, nasceu a digitalização da TV e, posteriormente, a possibilidade de transmissão pelas redes IP, conhecida como IPTV. Este trabalho analisa o cenário atual de IPTV para distribuição de conteúdo multimídia em redes de acesso privadas. Parte-se da necessidade de mecanismos de segurança para distribuição e controle do consumo das mídias digitais no sistema. O trabalho analisa, portanto, mecanismos para descrição inequívoca dos direitos que os usuários têm sobre o conteúdo adquirido, bem como a utilização de tecnologias para a transmissão do conteúdo de forma segura. O trabalho visa contribuir para a comunidade científica a partir da utilização de padrões abertos na especificação de um elemento de distribuição de vídeo em uma plataforma IPTV. Este elemento se baseia em políticas de uso da linguagem de expressão de direitos do MPEG-21 para reforçar os direitos de autoria e distribuição do conteúdo durante seu manuseio em toda a cadeia de negócios. / Internet consolidated IP networks as a versatile and accessible data transport infrastructure, thus justifying the growing interest on it as a transport network for several services, such as telephony and TV. The convergence leverages on the maturity of network technologies, responsible for the increasing availability of bandwidth for residential access, making the use of multimedia applications, including interactive ones, possible. From the demand for convergence, Digital TV has born, and later on, its transmission through IP networks, know as IPTV. This work analyzes current IPTV scenario for multimedia content distribution in the scope of private networks. It is based on the need for security mechanisms for digital content delivering and control of consumption in the system. Therefore it analyzes mechanisms for rights description, as well as security transmission of content. This work\'s contribution stems from the reutilization of open standards in the specification of a distribution broker in the IPTV environment. Such broker is based on the MPEG-21 digital rights description to enforce copyright of digital content during handling in the entire value chain.

Der Transkriptionsfaktor NF-κB ist von großer Bedeutung, da er verschiedene zelluläre Prozesse wie Proliferation, Apoptose, Invasion oder Inflammation beeinflusst. Im Gegensatz zu den meisten anderen Zelltypen ist in der humanen Epidermis ein wachstumsinhibierender Effekt mit der Aktivierung von NF-ĸB assoziiert. Die epidermale Homöostase dient der Aufrechterhaltung der intakten Hautbarriere und beschreibt das Gleichgewicht zwischen proliferierenden und differenzierenden epidermalen Keratinozyten. Schädliche äußere Einflüsse wie übermäßige Sonnenlichtexposition können die epidermale Homöostase stören, was zur Entstehung epidermaler Neoplasien wie aktinischer Keratosen oder Plattenepithelkarzinomen beiträgt. Das Ziel dieser Arbeit war die Expressions- und Funktionsanalyse der einzelnen NF-κB Proteinuntereinheiten in humanen Keratinozyten in vitro. Die siRNA-vermittelte transiente Reduktion von c-Rel beeinflusste deutlich das Zellschicksal von Keratinozyten. Obwohl vorangegangene Experimente durch eine stärkere Zellproliferation nach Inhibierung der NF-ĸB Proteine p50 und p65 das Gegenteil suggerierten, konnte für die Reduktion von c Rel ein inhibierender Effekt auf das Zellwachstum festgestellt werden. Außerdem zeigten sich eine veränderte Zellzyklusphasenverteilung sowie eine Akkumulation mitotischer Zellen mit aberranter, hauptsächlich monopolarer Spindelformation. Die zusätzlich detektierte Apoptose-Induktion könnte aus dem verlängerten mitotischen Arrest der c-Rel Knockdown Keratinozyten resultieren. Insgesamt lässt sich ein regulatorischer Effekt von c-Rel beim Eintritt in die Mitose oder der mitotischen Progression vermuten, wobei die beteiligten Zielgene noch zu identifizieren sind. Des Weiteren bewirkte der c-Rel Knockdown phänotypische Veränderung von HaCaT Keratinozyten mit tendenziell spindelzellartiger Elongation und einem insgesamt veränderten Wachstumsmuster. Die Adhäsion und besonders die Wundheilung von c-Rel reduzierten HaCaT Zellen war vermindert, möglicherweise bedingt durch ein reduziertes Stressfaservorkommen. Dieser Effekt zeigte sich allerdings nicht in c Rel herunter regulierten primären Keratinozyten, was auf Mutationen der spontan immortalisierten HaCaT Keratinozytenzelllinie zurückzuführen sein könnte. Zusammenfassend konnte in dieser Arbeit ein neuer Aspekt der einzelnen NF-ĸB Proteine aufgezeigt werden, besonders in Bezug auf die Proteinuntereinheit c-Rel. Hieraus resultiert ein besseres Verständnis der vielfältigen und komplexen Regulation von NF-κB abhängigen Funktionen und deren Effekte auf die epidermale Homöostase.

Induction of C-reactive protein (CRP) synthesis in hepatocytes by cytokines occurs at the transcriptional level. In Hep3B cells, the transcription factors C/EBPβ, STAT3, and Rel p50 have been shown to participate in this process. A C/EBP binding site centered at -53 and an overlapping nonconsensus κB site on the promoter are critical for CRP expression. We have previously found that an oligonucleotide containing a κB site diminished binding of C/EBPβ to the C/EBP site, suggesting that unidentified Rel proteins present in Hep3B nuclei facilitate the formation of C/EBPβ-complexes. The current studies were undertaken to determine which of the five Rel proteins, p50/p65/p52/c-Rel/RelB, play such a role. Mutation of the nonconsensus κB site did not abolish binding of C/EBPβ to its binding site, indicating that this site was not necessary for the formation of C/EBPβ-complexes. Depletion of Rel proteins from Hep3B nuclei led to decreased formation of C/EBPβ-complexes on a CRP promoter-derived oligonucleotide that contained only the intact C/EBP binding site but not the nonconsensus κB site. This finding indicates that Rel proteins are involved in the binding of C/EBPβ to its binding site by a κB site-independent mechanism. Electrophoretic mobility shift assays (EMSAs) revealed that it was c-Rel that facilitated formation of C/EBPβ-complexes and that c-Rel bound directly to C/EBPβ-complexes formed on the C/EBP site. Cotransfection of c-Rel enhanced the induction of CRP promoter-driven luciferase activity and enhanced endogenous CRP expression in cells transfected with C/EBPβ. We conclude that c-Rel regulates CRP expression without the requirement of binding to a κB site, and binds directly to C/EBPβ to facilitate the binding of C/EBPβ to the CRP promoter.

No description available.

Thesis (Ph.D.) - Carleton University, 2005. / Includes bibliographical references (p. 164-175). Also available in electronic format on the Internet.

No description available.

No description available.

Type 1 diabetes is an autoimmune disease resulting in the selective destruction of the insulin producing β-cells in the pancreas. Pro-inflammatory cytokines and the free radical nitric oxide (NO) have been implicated in mediating the destruction of β-cells, possibly through activation of the mitogen activated protein kinases (MAPKs) JNK, ERK and p38. In addition to MAPKs, cytokine signaling also results in activation of the transcription factor nuclear factor-kappaB (NF-κB). The upstream signaling events leading to MAPK and NF-κB activation in β-cells are not well known. The work presented in this thesis therefore aims at characterizing the regulation of MAPKs and NF-κB in human islets, with emphasis on the role of the MAPK activator MAP/ERK kinase kinase-1 (MEKK-1) in islet cell death. It was found that MEKK-1 was phosphorylated in response to the nitric oxide donor DETA/NONOate (DETA/NO), the β-cell toxin streptozotocin (STZ) and pro-inflammatory cytokines and that MEKK-1 downstream signaling in response to the same treatments involved activation of JNK but not ERK and p38. MEKK-1 was also found to be essential for cytokine-induced NF-κB activation. MEKK-1 downregulation protected human islet cells from DETA/NO-, STZ, and cytokine-induced cell death. Furthermore, overexpression of the NF-κB subunit c-Rel protected human islet cells from STZ and hydrogen peroxide-induced cell death indicating that NF-κB activity protects against cell death in human islets. In summary, these results support an essential role for MEKK-1 in the activation of JNK and NF-κB, with important consequences for human islet cell death and that strategies preventing human islets death by inhibition of the JNK pathway instead of NF-κB might be suitable.

Diss. (sammanfattning) Umeå : Univ., 2008. / Härtill 4 uppsatser.