Rights to some articles appeared in this disseration are reserved by the ELSEVIER B.V., John Wiley & Sons, Inc. and Pesticide Science Society of Japan.If any of these materials you wish to use, authorisation from sources must be obtain. / 京都大学 / 0048 / 新制・論文博士 / 博士(農学) / 乙第13023号 / 論農博第2833号 / 新制||農||1043(附属図書館) / 学位論文||H28||N4968(農学部図書室) / 32951 / (主査)教授 佐久間 正幸, 教授 松浦 健二, 教授 奥野 哲郎 / 学位規則第4条第2項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

Yes / This study examines the feasibility of using colloidal silica nanoparticles as active agents in high concentration waterborne polymer latex formulations. We showed that distributing the silica throughout the waterborne emulsion formed a composite coating material with a hydrophilic surface that consequently reduced exterior dirt pickup. Two grades of silica nanoparticles were studied, one using sodium stabilisation and another using epoxysilane modification to introduce glycidox-ypropyltrimethoxysilane surface functionality. Rheological study of the waterborne latex on mixing showed that there was an immediate pH responsive interaction between the silica sols and the polymer latex. Once loading of sodium charge stabilised silica NPs exceeded the volume required for heteroflocculation to occur the mixture demonstrated the potential to gel on standing – a process which took weeks, or months, to occur depending on the pH and relative concentrations used. At least fifty percent silane modification to the NP surface was found to be necessary to maintain a stable colloidal dispersion for long term storage of the waterborne latex. Despite this both grades of silica were found to imbue reductions in dirt pickup when applied to exterior masonry concrete studies over a 3-month weathering test / This work was supported by the Royal Society of Chemistry [E21-8346952505].

Climate change is leading to in an increase in frequency and severity of wildfires, which in turn can result in the formation of runoff-initiated post-wildfire debris flows. This type of debris flows is, like most debris flows, triggered by heavy precipitation events. Debris flows have the potential to cause much damage, and therefore it is important to analyse when and where the risk of these flows exists. This study aims to identify shared characteristics of basins that experienced post-wildfire debris flows in order to improve future risk analyses regarding such flows. These characteristics were studied through the analysis of 42 basins in 10 burned areas across western North America, which experienced a total of 67 post-wildfire debris flows between 2000 and 2018. Literature research and existing databases revealed the bedrock, soil texture and the timing of the flows compared to the wildfires. Spatial analysis using ArcMap allowed for the analysis of the burn severity of the basins, the hypsometry of the basins, and the mean slope of the basins. Analysis of these characteristics revealed the importance of the hypsometric integral, the soil texture, and the mean slope angle of the basins. In general, the hypsometric integral tends to fall between 0.50 and 0.60 and only soils with a coarse texture were identified for the basins. The mean slope angle of the basins is commonly between 25-30o, with a wider range of slopes being able to generate debris flows shortly after the fire. If multiple basins in a small area are burned, those with steeper slope angles have a higher potential to generate debris flows, while basins with steeper slopes do not have a higher risk on large regional scales. In order to generate post-wildfire debris flows the basin also needs to be burned at a large extent at low to medium severity, resulting in an extensive and strong water-repellent layer required to generate the runoff that is needed to generate a debris flow. Seasonal wetting during winters and drying of the soil during summers can reduce or enhance runoff respectively as well. As a result, post-wildfire debris flows occur mostly during the late summer months shortly after a wildfire when precipitation is increasing through summer storms, or a year later when the soil is dried and primed during the summer followed by such a summer storm. Fires during winter and thus outside the traditional wildfire season can lead to post-wildfire debris flows during winter as well due to the strength of the fresh water-repellent layer. Climate change which will lead to more fires during late autumn and winter months can thereby result in post-wildfire debris flows during winter, rather than only during the summer months following wildfires in the traditional fire season. / Jordskred uppstår när en sammanhängande jordmassa kommer i snabb rörelse. Det är en typ av naturolyckor som kan skada både infrastruktur och människor. Sannolikheten att ett jordskred inträffar ökar efter skogsbränder. Samtidigt kan klimatförändringar leda till en ökning av skogsbränder vilket i sin tur kan leda till en ökad risk för jordskred i framtiden. Syftet med det här projektet är att bidra till bedömningen av risken av jordskred till följd av skogsbänder i västra Nordamerika och att identifiera andra områden som också har en stor risk att drabbs av skred efter skogsbränder genom att identifiera riskfaktorer. Under projektets gång skapades en databas med områden där jordskred inträffade efter skogsbränder i västra Nordamerika. Den vetenskapliga litteraturen visade några egenskaper av skred och områdena, t.ex. när branden och skredet hände, vilken berggrund finns i området och texturen av jordarten i området. Dessutom användandes GIS (Geographical Information System) med satellitbilder och DEM (Digital Elevation models), som visade information om brandskador samt de geomorfologiska karaktärerna av områden. Resultaten visade att formationen av jordskred kräver omfattande låga till måttliga brandskador som resulterar i ett starkt vattenavvisande jordlager. Detta jordlager minskar infiltrationskapaciteten av jorden och resulterar i mer ytavrinning vilket orsakar skred till följd av brand. Även en grov textur av jordlagret är viktig eftersom den också bidrar till ett starkt vattenavvisande jordlager. Dessutom kan askpartiklar bli fångade in i stora porer i jord med en grov textur vilket minskar infiltrationskapaciteten och ökar ytavrinningen. Det finns alltså två krav för att ett jordskred ska inträffa efter en skogsbrand: i) omfattande låga-måttliga brandskador, och ii) en grov textur av jorden. Vidare finns det några ytterligare egenskaper som ökar risken för skred efter skogsbränder om de två kraven är uppfyllda. Den hypsometriska integralen (ett sätt att uttrycka hypsometrin av en dal) ligger oftast mellan 0.50-0.60 vilket är normal för en geomorfologiskt sett mogna område. Dessutom hade de flesta områdena i projektet en medellutning mellan 25o och 30o. Dock fanns det även tillfällen där värdena låg utanför dessa intervaller. Därför kan dessa värden i sig inte användas som riskfaktorer, utan borde de även kombineras med de övriga egenskaperna som beskrivs i den här undersökningen. Det är också relevant att veta när jordskred inträffar till följd av skogsbränder. Resultaten visade att skred kan hända strax efter skogsbränder i slutet av sommaren när det finns kraftigt regn. Det är då som det vattenavvisande jordlagret är som starkast. Det vattenavvisande lagret minskar i styrkan under året, men det är också möjligt att det inträffar ett jordskred under sommaren året efter en skogsbrand. Då är jorden torr i slutet av sommaren när kraftigt regn inträffar efter torra månader. Torr jorden bidrar även till en minskad infiltrationskapacitet. Regn i vinter gör jorden blöt vilket ökar infiltrationskapaciteten av jorden. Dessutom sker det även skogsbränder utanför den traditionella skogsbrandsäsongen nuförtiden, t.ex. tidigt i vintern. I så fall kan jordskred också inträffa under samma vinter strax efter skogsbranden, därför att det vattenavvisande jordlagret som skapas i branden fortfarande är starkt då. Som nämnts tidigare finns det en riskprofil som kan användas för att identifiera områden som har en hög risk för jordskred efter skogsbränder, men möjligheten att ett jordskred inträffar under vintern istället för (slutet av) sommaren bör även iakttas. Risken växer nämligen i samband med klimatförändringar som leder till mer skogsbränder; inte bara under sommaren, utan även under vintern.

Development of repellent surfaces which can supress bacteria adhesion, blood contamination and thrombosis, and non-specific adhesion on diagnostic devices has been a topic of intense research as these characteristics are in high demand. This thesis focused on design and development of omniphobic surfaces based on hierarchical structures and their application for preventing pathogenic contamination and biosensing. First, a flexible hierarchical heat-shrinkable wrap featuring micro and nanostructures, was developed with straightforward scalable methods which can be applied to existing surfaces. These surfaces reduced biofilm formation of World Health Organization-designated priority pathogens as well as minimized risk of spreading contamination from intermediate surfaces. This is due to the broad liquid repellency and the presence of reduced anchor points for bacterial adhesion on the hierarchical surfaces. Next, the developed surfaces were applied to minimize blood contamination and clot formation as well as facile integration of hydrophilic patterns. This led to droplet compartmentalization and was utilized for detection of Interleukin 6 in a rapid dip-based assay. Furthermore, in a review article the need for anti-viral or virus repellent surfaces and future perspectives were discussed as the global COVID-19 pandemic surged and attracted interest toward innovative technologies for suppressing the spread of pathogens. To address the pressing issue of non-specific adhesion in diagnostics devices, an omniphobic liquid infused electrochemical biosensor was developed. This was achieved by electroplating gold nanostructures on fluorosilanized gold electrodes. These electrodes demonstrated rapid and specific detection of Escherichia coli within an hour in complex biological liquids (blood, urine, etc.) without dilutions or amplification steps from clinical patient samples which are major bottle necks when rapid detection systems are sought for at the point of care. / Thesis / Doctor of Philosophy (PhD) / Repellent surfaces have a variety of applications in healthcare, for coating medical devices (e.g. indwelling implants, stethoscopes, and other external devices.), coating hospital surfaces for blood and pathogen repellency, and for developing anti-fouling diagnostic devices. Furthermore, they can be applied in the food sector for limiting contaminations, and in public areas on high-touch surfaces to eliminate the spread of infection. Therefore, there is a need for repellent surface which can be easily applied to surfaces with various form factors while having an easy fabrication method. Featuring hierarchical structures on a heat-shrinkable material, a repellent wrap was designed to be integrated on existing surfaces and repel pathogens and suppress the spread of infection as an intermediate surface. Similar concept was used for designing blood repellent surfaces which were patterned with hydrophilic regions for a rapid dip-based biosensing platform. Finally, surface textures on conductive materials with liquid infused repellent coatings were investigated for electrochemical biosensing in complex biological liquids.

Textile processing methods such as conventional exhaustion dyeing, pre-treatments and printing consume high amounts of water and use partly toxic and hazardous chemicals which are non-degradable. These chemicals (e.g. excess amount of dye, additives and catalysts) remain partially in the waste-water which is drained out and ends up polluting the environment. The supercritical carbon dioxide dyeing technology presents an eco-friendly and water-free method with reduced use of chemicals and energy. The benefits of such technology are currently not overcoming the relative high investment costs which impede its full implementation into the textile industry. This study presents an approach to extent the application of the eco-friendly supercritical carbon dioxide technology. It combines the well-studied supercritical carbon dioxide dyeing process for polyester with the functionalisation process to obtain water repellent surface properties. As water repellent (substance) environmentally benign silicones are used. Results showed that the simultaneous dyeing and functionalisation process was feasible assessed by the compatibility of the dye and silicone in the system. Silicone and dye did not interfere in each other’s functionality (colour strength and water contact angle). Further the process temperature and silicone molecular weight showed no influence on the colour strength of the fabric whereas the water contact angle (water repellence) increased with increasing temperature. The resulting polyester fabric showed acceptable colour strength yet did not obtain sufficient water repellent properties despite the increase in water contact angle of the treated samples to the untreated reference sample. The poor water repellence is suggested to be caused by the hydrophobic functional groups of the silicones oriented towards each other rather than toward the outer fabric surface. Overall the thesis is promoting research which combines eco-friendly technologies including environmental benign chemicals for the textile industry. Silicones are widely used in textile processing not only as water repellents, but also as anti-foaming agents, lubricants and softeners. Therefore a water-free and eco-friendly application method can benefit a wide range of finishing processes.

<p>A computer model based on minimization of the free energy, capable to predict contact angles and spreading transitions between Wenzel and Cassie mode for drops placed on surfaces with different topography were implemented in matlab. Simulations were compared with experiments documented in the literature. These showed that reported transitions between Cassie and Wenzel mode can be explained by minimization of the free energy. In this report, a study on the possibility of constructing water repellent paper surfaces with a combination of treatment with octadecyltrichlorosilane and topography changes, is included.</p>

A computer model based on minimization of the free energy, capable to predict contact angles and spreading transitions between Wenzel and Cassie mode for drops placed on surfaces with different topography were implemented in matlab. Simulations were compared with experiments documented in the literature. These showed that reported transitions between Cassie and Wenzel mode can be explained by minimization of the free energy. In this report, a study on the possibility of constructing water repellent paper surfaces with a combination of treatment with octadecyltrichlorosilane and topography changes, is included.

The report is a comparative study between spruce plantations where 4 plants are treated with deer repellents and 4 plantations are untreated. The study aims to provide a base for the Forest Society's future decisions regarding the use of wildlife-repellents.  The report indicates that wildlife-repellents work reasonably well for its purpose, the treated stocks were virtually untouched during the planting year and next year while the untreated stocks have been heavily grazed during these years.  Growth difference between grazed plants and ungrazed plants found to be virtually non-existent (0, 01 m) of the treated stocks and 0, 07 m in the untreated plants. Because of the growth differences are so small between grazed and no grazed plants, it seems like the treatment of deer-repellent are unnecessary. Unless you know in particularly that the area has a specific problem whit severe deer-grazing.

This study examined the role of olfactory and visual cues in host finding of the pine bark beetles Hylastes ater and Hylurgus ligniperda and the burnt pine longhorn beetle Arhopalus ferus. The ultimate aim of this research was to provide new information on attractant and repellent (such as non-host leaf volatiles) stimuli to improve monitoring methods and reduce the attack by wood-boring and bark beetle species. A field trapping trial of visual and olfactory cues near Nelson caught 7842 H. ater, 274,594 H. ligniperda and 16,301 A. ferus adults. There were significant effects of both visual (colour and sihoutte) and olfactory (host and non-host volatiles) cues for all three species. The highest catches were in black (host mimicking), panel flight intercept traps baited with attractant (α-pinene and ethanol) and the lowest in clear or white (non-host mimicking) control traps. The repellent, green leaf volatiles (GLV) ((E)-2-hexen-1-ol & (Z)-3-hexen-1-ol) when present on traps with attractant reduced catches significantly but modestly in H. ater and H. ligniperda, but had no significant effect on A. ferus. A field trial near Christchurch found that GLV applied as a topical repellent halved the number of beetles attacking Pinus radiata logs. This reduction was significant in H. ligniperda, but not quite (P = 0.07) in H. ater. Placing logs among broadleaved plants (natural sources of non-host volatiles) significantly reduced attack of H. ligniperda by about 75% compared to logs in the open, but had no effect on H. ater. Attack by H. ater was found on 4% of 500 P. radiata seedlings in a field trial near Dunedin. Treatment of seedlings with GLV significantly affected the severity and proportion of seedlings attacked by H. ater, compared with insecticide-treated and control seedlings, but the treatment effect was apparently driven by an unexpected direct damaging effect of GLV on the health of seedlings. It is recommended that future research explores the use of non-host volatiles from natural sources that influence host finding in wood–boring and bark beetle species for the protection of plantation forests in New Zealand.

This study investigated the potential of companion planting as a strategy to aid in the management of Helicoverpa armigera in tomato and capsicum cropping systems. The investigaitons showed that a companion planting type system is unlikely to confer any benefits to the management of H. armigera in these crops. The project has contributed to the understanding of H. armigera host selection and how the pest responds to a diversified environment. Companion planting and vegetational diversity are strategies for reducing pest incidence on crops. Many theories have been devised to explain how introducing more than one plant species to a cropping space may reduce the incidence of a pest. Five ecological theories formed the basis of experiments in this study: The resource concentration hypothesis - Herbivores are more likely to find and remain on hosts that are growing in dense or nearly pure stands (Root, 1973). Associational resistance/Plant apparency - Crop plants grown as monocultures are more apparent to herbivorous insects than plants in diverse natural systems (Tahvanainen and Root, 1972). Trap cropping – the use of plants within a cropping area to attract oviposition away from the main crop (Banks and Ekbom, 1999). The ability of H. armigera to learn in regard to host seleciton (Cunningham et al., 1998a). The natural enemies hypothesis - generalist and specialist natural enemies are expected to be more abundant in diverse rather than simple systems (Root, 1973) A tall variety of sorghum (the forage variety Chopper) was tested for its ability to disrupt host location. Sorghum was planted around plots of tomatoes to act as a screen to disrupt visual cues for host location by Helicoverpa spp. No difference was found in egg numbers on tomatoes with or without sorghum companions. However, significantly higher numbers of Helicoverpa spp. larvae were found in the sorghum at flowering than in the tomatoes. This suggested the sorghum was acting as a trap crop, diverting oviposition away from the tomato crop. However, due to a short period of flowering and therefore peak attractiveness to H. armigera, sorghum was not considered to be a suitable companion plant for inclusion in commercial tomato production systems. A host preference study was conducted to determine the preferences of H. armigera for crop plants and possible companions in the hope of finding a suitable trap crop species. Tomatoes were shown to be a highly preferred plant making it difficult to find a compatible trap crop species that would be more attractive than the main crop. However, capsicums were less prefered and therefore more suitable for this type of experimentation. Marigolds were also found to be a highly preferred plant and formed the basis for further investigations into trap cropping systems. Field experiments were conducted in successive years in two geographical locations to assess the suitability of marigolds as a trap crop for capsicums in a field situation. However, in both years H. armigera incidence was extremely and unusually low leading to inconclusive results. A glasshouse experiment was performed to assess if the ratio of capsicum plants to marigolds plants had an effect on which species H. armigera would choose for oviposition. It was found that as more capsicum plants were introduced, moths became less likely to oviposit on the supposedly more preferred marigold plant. The diminishing attractiveness of a more preferred host in the presence of other hosts is a new observation of H. armigera behaviour. This result suggests that marigolds would be unlikely to be a successful trap crop in a field situation. The ability of H. armigera to learn in regard to host selection and the influence of this on host selection in the field was investigated. No evidence of learning was found. This was the first study investigating the effect of learning in a field situation; previously the behaviour had only been investigated in a laboratory situation (Cunningham, et al. 1998a). The ability of the Australian assassin bug, Pristhesancus plagipennis (Walker) (Hemiptera: Reduviidae) to predate H. armigera larvae on capsicum plants was investigated. This predator has been sucessfully been used for H. armigera management in cotton (Grundy, 2000b), but had not been investigated in horticultural crops. Significant reductions in larvae were achieved in treatments where assassin bugs were introduced. This predator warrants further investigation for inclusion in commercial integrated pest managment programs for capsicums. Pest repellent plants were also considered. Previous to this study, very little research work had been conducted on repellent plants for H. armigera. The herbs investigated were catnip (Nepta cataria), tansy (Tanacetum vulgare), basil (Ocium basilicum) and coriander (Coriander sativum). An olfactometer system was designed and built to test H. armigera moths’ responses to odours from the herbs. Glasshouse experiments were also conducted. No repellent activity was recorded with any of the herbs tested. The results of this study support the theory that Helicoverpa spp. employ a strategy of passive host selection as suggested by Firempong (1986). This means that all available plants in an area may be oviposited on with successful ovipostion and larval development leading to the future utilisation of a species as a host plant. The implication of this finding is that in-field trap cropping is unlikely to be a successful strategy for reducing H. armigera oviposition in tomato or capsicum production systems in Australia. In cotton crops in Australia, trap cropping is used as part of an area wide management strategy and aims to reduce the total Helicoverpa spp. population of a region. Such a strategy may also be successful in horticultural crops but only with the full participation of the growers of all crops that are attractive to H. armigera in a horticultural growing region. Due to the diverse nature of horticultural production this may be a difficult task.