INTRODUÇÃO: As Aeromonas são bactérias distribuídas predominantemente em meio aquático. São consideradas patógeno emergente, podendo causar doenças em peixes como também no homem. Os problemas mais comuns são a gastrenterite no homem e morte em peixes. OBJETIVO: Este estudo foi desenvolvido para comparar a identificação fenotípica com a genotípica, e também para conhecer o perfil de resistência aos antibióticos em Aeromonas caviae, A. aquariorum, e A. sanarellii isoladas do ambiente aquático e a presença de genes de virulência e resistência. MATERIAL E MÉTODOS: O DNA das 24 cepas em estudo foi extraído por choque térmico e purificado utilizando CTAB. Foram realizadas as PCRs para a detecção dos genes de virulência e dos genes de resistência, após a realização do antibiograma. RESULTADOS: Foram identificadas 4 A. caviae das quais 3(75,0 por cento) apresentaram pelo menos um dos genes act, ast ou alt. Das 3 A. aquariorum, 1(33,3 por cento) apresentaram positividade para os genes act e ast. Entre os 5 isolados de A. sanarellii 1(50,0 por cento) possuíam os genes alt e ast. Seis isolados não foram posicionados taxonomicamente entre as espécies descritas de Aeromonas, e dentre essas um exemplar apresentou o gene alt. Em relação às enzimas MBL e AmpC foram obtidos respectivamente: 3(100 por cento) e 3(100 por cento) em A. aquariorum; 2(50,0 por cento) e 4(100 por cento) em A. caviae; 3(75,0 por cento) e 5(100 por cento) em Aeromonas spp.; 1(20 por cento) e 5(100 por cento) A. sanarellii; Nenhum isolado apresentou resultado positivo, no teste fenotípico, para a produção de ESBL. Com a realização da PCR foi detectada a presença de 5 amostras com gene tipo blaMOX, 21blaCPHA , 17 tipo blaTEM e 2 cepH. CONCLUSÃO: Os resultados sugerem que os isolados podem servir potencialmente como reservatórios de resistência aos antimicrobianos e ainda, que os isolados podem ser considerados patógeno emergentes e significativos para a saúde pública / INTRODUCTION: Aeromonas spp. is predominantly distributed in the aquatic environment. They are regarded as emerging pathogen, causing disease in fish but also in man. The most common problems are gastroenteritis in humans and death in fish. OBJECTIVE: This study was designed to compare phenotypic with genotypic identification, and also to know the profile of antibiotic resistance in Aeromonas caviae, A. aquariorum, and A. sanarellii isolated from aquatic environment and the presence of virulence genes and resistance. MATERIAL AND METHODS: DNA from 24 strains under study was extracted by thermal shock and purified using CTAB. PCR reactions were performed for the detection of virulence and resistance genes, after the completion of the antibiotic resistance test. RESULTS: We identified four A. caviae from which 3(75.0per cent) had at least one of the genes act, ast or alt. From 3 A. aquariorum, 1(33.3per cent) was positive for the genes act and ast. Among the five isolated A. sanarellii, 1(50.0per cent) had the alt and ast genes Six isolates were not positioned within taxonomically described species of Aeromonas, and among these only one strain presented the alt gene. Regarding the MBL and AmpC it was obtained respectively: 3(100per cent) and 3(100per cent) isolates from A. aquariorum; 2(50.0per cent) and 4(100per cent) isolates from A. caviae; 4(66.7per cent) and 3(50.0per cent) isolates from Aeromonas spp.; and 1(20per cent) and 5 (100per cent) isolates from A. sanarellii. None of the isolates showed positive results in the phenotypic test for ESBL production. The PCR reaction detected the presence of 5 strains with blaMOX-like gene; 21 with blaCPHA gene; 17 with blaTEM-like gene and 2 with cepH gene. CONCLUSION: These findings suggest that the isolates may serve as potential reservoirs of antimicrobial resistance and also that the isolates could be considered emerging pathogens of public health significance

INTRODUÇÃO: As Aeromonas são bactérias distribuídas predominantemente em meio aquático. São consideradas patógeno emergente, podendo causar doenças em peixes como também no homem. Os problemas mais comuns são a gastrenterite no homem e morte em peixes. OBJETIVO: Este estudo foi desenvolvido para comparar a identificação fenotípica com a genotípica, e também para conhecer o perfil de resistência aos antibióticos em Aeromonas caviae, A. aquariorum, e A. sanarellii isoladas do ambiente aquático e a presença de genes de virulência e resistência. MATERIAL E MÉTODOS: O DNA das 24 cepas em estudo foi extraído por choque térmico e purificado utilizando CTAB. Foram realizadas as PCRs para a detecção dos genes de virulência e dos genes de resistência, após a realização do antibiograma. RESULTADOS: Foram identificadas 4 A. caviae das quais 3(75,0 por cento) apresentaram pelo menos um dos genes act, ast ou alt. Das 3 A. aquariorum, 1(33,3 por cento) apresentaram positividade para os genes act e ast. Entre os 5 isolados de A. sanarellii 1(50,0 por cento) possuíam os genes alt e ast. Seis isolados não foram posicionados taxonomicamente entre as espécies descritas de Aeromonas, e dentre essas um exemplar apresentou o gene alt. Em relação às enzimas MBL e AmpC foram obtidos respectivamente: 3(100 por cento) e 3(100 por cento) em A. aquariorum; 2(50,0 por cento) e 4(100 por cento) em A. caviae; 3(75,0 por cento) e 5(100 por cento) em Aeromonas spp.; 1(20 por cento) e 5(100 por cento) A. sanarellii; Nenhum isolado apresentou resultado positivo, no teste fenotípico, para a produção de ESBL. Com a realização da PCR foi detectada a presença de 5 amostras com gene tipo blaMOX, 21blaCPHA , 17 tipo blaTEM e 2 cepH. CONCLUSÃO: Os resultados sugerem que os isolados podem servir potencialmente como reservatórios de resistência aos antimicrobianos e ainda, que os isolados podem ser considerados patógeno emergentes e significativos para a saúde pública / INTRODUCTION: Aeromonas spp. is predominantly distributed in the aquatic environment. They are regarded as emerging pathogen, causing disease in fish but also in man. The most common problems are gastroenteritis in humans and death in fish. OBJECTIVE: This study was designed to compare phenotypic with genotypic identification, and also to know the profile of antibiotic resistance in Aeromonas caviae, A. aquariorum, and A. sanarellii isolated from aquatic environment and the presence of virulence genes and resistance. MATERIAL AND METHODS: DNA from 24 strains under study was extracted by thermal shock and purified using CTAB. PCR reactions were performed for the detection of virulence and resistance genes, after the completion of the antibiotic resistance test. RESULTS: We identified four A. caviae from which 3(75.0per cent) had at least one of the genes act, ast or alt. From 3 A. aquariorum, 1(33.3per cent) was positive for the genes act and ast. Among the five isolated A. sanarellii, 1(50.0per cent) had the alt and ast genes Six isolates were not positioned within taxonomically described species of Aeromonas, and among these only one strain presented the alt gene. Regarding the MBL and AmpC it was obtained respectively: 3(100per cent) and 3(100per cent) isolates from A. aquariorum; 2(50.0per cent) and 4(100per cent) isolates from A. caviae; 4(66.7per cent) and 3(50.0per cent) isolates from Aeromonas spp.; and 1(20per cent) and 5 (100per cent) isolates from A. sanarellii. None of the isolates showed positive results in the phenotypic test for ESBL production. The PCR reaction detected the presence of 5 strains with blaMOX-like gene; 21 with blaCPHA gene; 17 with blaTEM-like gene and 2 with cepH gene. CONCLUSION: These findings suggest that the isolates may serve as potential reservoirs of antimicrobial resistance and also that the isolates could be considered emerging pathogens of public health significance

Orientador: Antonia Dalla Pria Bankoff / Dissertação (mestrado) - Universidade Estadual de campinas, Faculdade de Educação Fisica / Made available in DSpace on 2018-08-06T18:13:13Z (GMT). No. of bitstreams: 1 GallardoGreen_OrlandoSantiago_M.pdf: 294982 bytes, checksum: 5ea6fc1a4ecca7e9d25a0c8e4205568d (MD5) Previous issue date: 2004 / Resumo: O objetivo do presente estudo foi medir a Resistência Aeróbica através do Teste de Mazorra Zamora em adultas maiores, da cidade de Concepción¿Coronel - Chile, onde a mostra foi de pessoas voluntárias, selecionadas de um grupo de Ginástica para o Adulto Maior Ativas de: Caminho Olavarría integrada por 5 senhoras; um grupo de Ginástica do Departamento de Educação Física da Universidade de Concepción, integrada por 15 senhoras; outro grupo pertecente ao Consultório Víctor Manuel Fernández integrado por 24 senhoras e um grupo de 18 adultas maiores sedentárias do Club ¿San Francisco de Asís¿ de Coronel e uma adulta maior sedentária voluntária de Concepción. O Teste aplicado se caracteriza por dar resultados em distância recorrida num tempo predeterminado. Por otro lado, se utilizou estatística descritiva de média, desviação estándar e nível para caracterizar aos grupos e a aplicação da fórmula ¿t de student¿ que permitiu ratificar que há diferença significativa entre adultos maiores que praticam atividade física e aqueles adultos maiores que não praticam atividade física / Abstract: The target of the present study was to measure the Aerobic Resistance by means of the Mazorra-Zamora Test in senior citizens of the city of Concepción-Coronel ¿ Chile. The sample was made up of volunteers. The people were chosen from a Gymnastics-for-the-Active-Elderly group in the Camilo Olavarría neighbourhood (6 women); a Gymnastics Group from the Department of Physical Education of the University of Concepción (15 women); a group from the Víctor Manuel Fernández Health Care Center (24 women); a group of sedentary elderly women (18) from the San Francisco de Asís Club in the city of Coronel; and one volunteer, elderly, sedentary woman from the city of Concepción. The Test applied is characterized by its results given in reference to a certain distance covered in a predetermined period of time. On the other hand, average descriptive statistics, standard deviation and rank were used to characterize the groups and the application of the ¿t of student¿ Test, which allowed the substantiation that there is significant difference between seniors citizens engaged in physical activity and those who are not engaged in physical activity / Mestrado / Ciencia do Desporto / Mestre em Educação Física

The ASTM E119 is a large-scale test used to qualify assemblies for fire resistance, including heat transmission and structural integrity. The test requires specialized furnaces and full-scale assemblies that are 3.0 m (10 ft) or more on each side, making it very expensive to perform. In this study, we investigated the feasibility of the scaling methodology for a reduced-scale fire resistance test on different types of wood-based structures, specifically commercially available intumescent coating applied onto wood and bolted lap joints in wood. We build upon a previously developed scaling methodology for wood and gypsum boards, which integrated geometric scaling, Fourier number time scaling, and furnace boundary condition matching. Intumescent coating presents a particular challenge in scaling in that it expands when exposed to fire conditions. To account for this expansion, we identified a relationship between initial dry film thickness and final expanded thickness through cone calorimeter tests and integrated it into a modified scaling methodology. This approach was then validated through fire exposure tests in furnace on wood samples painted with intumescent coating at full, half, and quarter scales. Finally, we demonstrated the scaling laws for joints under combined thermo-structural loading, by subjecting wood-based half-lap joint samples to combined bending and thermal loading at half and quarter scale. The samples were subjected to static three-point bending with the load scaled to achieve structural similitude, while simultaneously being exposed to a scaled fire exposure on the bottom surface. Our study provides insights into the practical application of scaling methodology for testing the fire resistance of joints and fire-resistant coated wood, paving the way for more cost-effective and quicker fire testing for the wood-based composite industry. / Master of Science / The ASTM E119 is a critical test standard that evaluates the fire resistance of various building materials, including wood-based structures. However, the standard tests are quite expensive due to the need for specialized equipment and large-scale samples. In this study, we explored the potential of using a scaled-down fire resistance test on different types of wood-based materials, including commercially available fire-resistant coated wood and joints. We built on existing scaling methods for wood and gypsum boards and adapted it to account for the unique properties of intumescent coating - a fire-resistant material that expands when exposed to high temperatures. By conducting a series of tests, we developed a modified scaling approach to accommodate the expansion of the coating. We then validated this new method by performing fire exposure tests at various scales on wood samples coated with intumescent coating. Finally, we adapted the scaling methods to account for wood based bolted joints. We tested the fire resistance of wood-based half-lap joints under combined heat and structural stress at smaller scales. Our study offers valuable insights into a more cost-effective and efficient method for testing fire resistance in wood-based structures. By providing a reliable scaling approach for fire-resistant coated wood and joints, our work has the potential to make fire testing more accessible for the wood composite industry, ultimately leading to safer and better-performing buildings.

Tire testing has been one of the important aspects of the tire industry because it helps identify the tire behavior which further helps in improving the design of tires. It also helps automotive manufacturers choose the best tire for their automobiles. Indoor tire testing helps in relating the data better because of greater repeatability of the testing setup as compared to outdoor testing. This study focusses on modifying a rolling resistance machine to make it capable of force and moment and cleat testing along with the standard rolling resistance test. Additionally, the design of a mechanical loading mechanism (used to apply normal force on the tire) in place of the previous one using dead weights is also discussed. This study also talks about the structural and vibrational finite element analysis of a tire testing machine. Since the machine was designed to conduct different tire tests, different structural requirements of the tire positioning mechanism pertaining to each test were taken into consideration, and the structure was analyzed for maximum forces and moments acting on the assembly. Cleat testing subjects the tire as well as the structure to an impulse force which calls for the vibrational analysis of the assembly to avoid the structure from resonating. The design was modified to get it easily manufactured and assembled. These design changes and the aspects taken into consideration have also been discussed. / Master of Science

A partir de um alerta internacional emitido pelas autoridades sanitárias francesas no ano de 2010 com respeito a altas taxas de ruptura de implantes mamários da marca Poly Implant Prothèse (PIP), a Agência de Vigilância Sanitária brasileira (ANVISA) suspendeu a importação, distribuição, comércio e uso destes implantes no país. Visando manter a qualidade destes produtos no mercado brasileiro, a partir de março de 2012 – conforme publicado pelo Instituto Nacional de Metrologia (INMETRO) – foi instituída a certificação compulsória dos implantes mamários e em seguida definidos os ensaios através dos quais os implantes mamários devem ter sua conformidade avaliada, sendo esses ensaios mecânicos e químicos. Este trabalho apresenta o desenvolvimento de equipamentos e metodologia para avaliação da conformidade de dois ensaios mecânicos em implantes mamários: ensaio de fadiga e ensaio de resistência ao impacto. Duas normas foram utilizadas para o processo de acreditação: ISO 14607 – Non - active surgical implants – Mammary implants – Particular Requirements, e ABNT NBR ISO 14607 – Implantes cirúrgicos não ativos – Implantes mamários – Requisitos particulares. Os equipamentos utilizados foram desenvolvidos e construídos ao longo do desenvolvimento deste estudo e os testes foram realizados em implantes mamários da marca PIP obtidos junto a ANVISA. A primeira etapa consistiu em organizar os documentos necessários para acreditação junto ao INMETRO e, após, ensaios os ensaios foram realizados. Ao todo trinta e cinco implantes foram ensaiados – 31 na primeira parte do trabalho e 04 na segunda – e analisados em lupa para verificação de defeitos causados pelos testes como: fraturas, cortes, alteração de forma, entre outros. Os equipamentos e metodologia desenvolvida atendem os requisitos de ambas as normas e permitem avaliação de conformidade de próteses mamárias comercializadas no país, no que diz respeito ao ensaio de fadiga e resistência ao impacto. / Due an international alert issued by French authorities in 2010 regarding high rupture rate of breast implants related to Poly Implant Prothèse (PIP), the Brazilian Health Surveillance Agency has suspended the importation, distribution, commercialization and use of these implants into Brazil. In order to maintain the quality of these products in Brazilian market, on March 2012 – according published by National Institute of Metrology – it has been decided for compulsory certification of breast implants and then defined the tests through which these products have to be submitted for its conformity assessment, being these mechanical and chemical tests. This dissertation evaluates the development and methodology for conformity assessment of two mechanical tests on breast implants: fatigue and impact resistance test. The process was based on two standards: ISO 14607 and ABNT NBR ISO – Non - active surgical implants – Mammary implants – Particular Requirements. The equipment used was developed in the laboratory during the realization of this study and the tests were performed on implants produced by PIP provided by National Sanitary Surveillance Agency. It started with the organization of the documents to be sent to National Institute of Metrology, in order to ask the accreditation and then tests were performed. Thirty five implants were tested – 31 on first part of the work and 04 on the second one – and then analyzed to detect any anomaly that could be considered a defect such as: rupture, cuts, shape alteration, etc. The equipment and methodology has met the requirements from both standards and enable conformity assessment of breast implants commercialized in Brazil, regarding to fatigue and impact resistance tests.

A partir de um alerta internacional emitido pelas autoridades sanitárias francesas no ano de 2010 com respeito a altas taxas de ruptura de implantes mamários da marca Poly Implant Prothèse (PIP), a Agência de Vigilância Sanitária brasileira (ANVISA) suspendeu a importação, distribuição, comércio e uso destes implantes no país. Visando manter a qualidade destes produtos no mercado brasileiro, a partir de março de 2012 – conforme publicado pelo Instituto Nacional de Metrologia (INMETRO) – foi instituída a certificação compulsória dos implantes mamários e em seguida definidos os ensaios através dos quais os implantes mamários devem ter sua conformidade avaliada, sendo esses ensaios mecânicos e químicos. Este trabalho apresenta o desenvolvimento de equipamentos e metodologia para avaliação da conformidade de dois ensaios mecânicos em implantes mamários: ensaio de fadiga e ensaio de resistência ao impacto. Duas normas foram utilizadas para o processo de acreditação: ISO 14607 – Non - active surgical implants – Mammary implants – Particular Requirements, e ABNT NBR ISO 14607 – Implantes cirúrgicos não ativos – Implantes mamários – Requisitos particulares. Os equipamentos utilizados foram desenvolvidos e construídos ao longo do desenvolvimento deste estudo e os testes foram realizados em implantes mamários da marca PIP obtidos junto a ANVISA. A primeira etapa consistiu em organizar os documentos necessários para acreditação junto ao INMETRO e, após, ensaios os ensaios foram realizados. Ao todo trinta e cinco implantes foram ensaiados – 31 na primeira parte do trabalho e 04 na segunda – e analisados em lupa para verificação de defeitos causados pelos testes como: fraturas, cortes, alteração de forma, entre outros. Os equipamentos e metodologia desenvolvida atendem os requisitos de ambas as normas e permitem avaliação de conformidade de próteses mamárias comercializadas no país, no que diz respeito ao ensaio de fadiga e resistência ao impacto. / Due an international alert issued by French authorities in 2010 regarding high rupture rate of breast implants related to Poly Implant Prothèse (PIP), the Brazilian Health Surveillance Agency has suspended the importation, distribution, commercialization and use of these implants into Brazil. In order to maintain the quality of these products in Brazilian market, on March 2012 – according published by National Institute of Metrology – it has been decided for compulsory certification of breast implants and then defined the tests through which these products have to be submitted for its conformity assessment, being these mechanical and chemical tests. This dissertation evaluates the development and methodology for conformity assessment of two mechanical tests on breast implants: fatigue and impact resistance test. The process was based on two standards: ISO 14607 and ABNT NBR ISO – Non - active surgical implants – Mammary implants – Particular Requirements. The equipment used was developed in the laboratory during the realization of this study and the tests were performed on implants produced by PIP provided by National Sanitary Surveillance Agency. It started with the organization of the documents to be sent to National Institute of Metrology, in order to ask the accreditation and then tests were performed. Thirty five implants were tested – 31 on first part of the work and 04 on the second one – and then analyzed to detect any anomaly that could be considered a defect such as: rupture, cuts, shape alteration, etc. The equipment and methodology has met the requirements from both standards and enable conformity assessment of breast implants commercialized in Brazil, regarding to fatigue and impact resistance tests.

A partir de um alerta internacional emitido pelas autoridades sanitárias francesas no ano de 2010 com respeito a altas taxas de ruptura de implantes mamários da marca Poly Implant Prothèse (PIP), a Agência de Vigilância Sanitária brasileira (ANVISA) suspendeu a importação, distribuição, comércio e uso destes implantes no país. Visando manter a qualidade destes produtos no mercado brasileiro, a partir de março de 2012 – conforme publicado pelo Instituto Nacional de Metrologia (INMETRO) – foi instituída a certificação compulsória dos implantes mamários e em seguida definidos os ensaios através dos quais os implantes mamários devem ter sua conformidade avaliada, sendo esses ensaios mecânicos e químicos. Este trabalho apresenta o desenvolvimento de equipamentos e metodologia para avaliação da conformidade de dois ensaios mecânicos em implantes mamários: ensaio de fadiga e ensaio de resistência ao impacto. Duas normas foram utilizadas para o processo de acreditação: ISO 14607 – Non - active surgical implants – Mammary implants – Particular Requirements, e ABNT NBR ISO 14607 – Implantes cirúrgicos não ativos – Implantes mamários – Requisitos particulares. Os equipamentos utilizados foram desenvolvidos e construídos ao longo do desenvolvimento deste estudo e os testes foram realizados em implantes mamários da marca PIP obtidos junto a ANVISA. A primeira etapa consistiu em organizar os documentos necessários para acreditação junto ao INMETRO e, após, ensaios os ensaios foram realizados. Ao todo trinta e cinco implantes foram ensaiados – 31 na primeira parte do trabalho e 04 na segunda – e analisados em lupa para verificação de defeitos causados pelos testes como: fraturas, cortes, alteração de forma, entre outros. Os equipamentos e metodologia desenvolvida atendem os requisitos de ambas as normas e permitem avaliação de conformidade de próteses mamárias comercializadas no país, no que diz respeito ao ensaio de fadiga e resistência ao impacto. / Due an international alert issued by French authorities in 2010 regarding high rupture rate of breast implants related to Poly Implant Prothèse (PIP), the Brazilian Health Surveillance Agency has suspended the importation, distribution, commercialization and use of these implants into Brazil. In order to maintain the quality of these products in Brazilian market, on March 2012 – according published by National Institute of Metrology – it has been decided for compulsory certification of breast implants and then defined the tests through which these products have to be submitted for its conformity assessment, being these mechanical and chemical tests. This dissertation evaluates the development and methodology for conformity assessment of two mechanical tests on breast implants: fatigue and impact resistance test. The process was based on two standards: ISO 14607 and ABNT NBR ISO – Non - active surgical implants – Mammary implants – Particular Requirements. The equipment used was developed in the laboratory during the realization of this study and the tests were performed on implants produced by PIP provided by National Sanitary Surveillance Agency. It started with the organization of the documents to be sent to National Institute of Metrology, in order to ask the accreditation and then tests were performed. Thirty five implants were tested – 31 on first part of the work and 04 on the second one – and then analyzed to detect any anomaly that could be considered a defect such as: rupture, cuts, shape alteration, etc. The equipment and methodology has met the requirements from both standards and enable conformity assessment of breast implants commercialized in Brazil, regarding to fatigue and impact resistance tests.

Submitted by Marilene Donadel (marilene.donadel@unioeste.br) on 2017-11-08T23:52:44Z No. of bitstreams: 1 Stefane_S_Corrêa_2017.pdf: 1344962 bytes, checksum: 2369f5181c7145ed68be3f6f7181b3f8 (MD5) / Made available in DSpace on 2017-11-08T23:52:44Z (GMT). No. of bitstreams: 1 Stefane_S_Corrêa_2017.pdf: 1344962 bytes, checksum: 2369f5181c7145ed68be3f6f7181b3f8 (MD5) Previous issue date: 2017-03-24 / The leather tanning of fish is a way of alleviating the damage caused by chromium salts to the environment. this work had as objective evaluate the process of tanning leather in the skins in corvina and pescada amarela from fishing made in the northern region of Brasil and tilápia origin of fish farming. The species caught in the fishing of the region present the potential for use in the tanning because they are large, which allows you to add value to the final product. The skins were to the same tanning process not differing in any of its stages. to determine the efficiency of the process, the skins of corvina, pescada amarela and tilapia were submitted to the resistence test the traction, stretching and progressive tear in the longitudinal and transverse directions of the body of the fish and the physical-chemical analysis. the resistance tests the traction and stretching were satisfactory for manufacturing of products and clothing. However, suggests improve the grease of the tanning process. / O curtimento vegetal de couro de peixes é uma forma de amenizar os danos causados pelos sais de cromo ao ambiente. Este trabalho teve como objetivo avaliar o processo de curtimento nas peles de corvina e pescada amarela oriundas da pesca realizadas na região norte e tilápia oriunda de piscicultura do estado do Paraná. As peles foram submetidas ao mesmo processo de curtimento vegetal não diferindo em nenhuma das suas etapas. Para determinar a eficiência do processo, as peles de corvina, pescada amarela e tilápia foram submetidas à análise físico-química, ao teste de resistência à tração, alongamento e rasgamento progressivo no sentido longitudinal e transversal do corpo do peixe. Foi realizada a analise histológica dos couros, para se analisar a estrutura das fibras. Os testes de resistência à tração e alongamento foram satisfatórios, comprovando que as peles das espécies capturadas na pesca da região norte do Brasil apresentam potencial para utilização no curtimento, apresentando qualidade apropriada para confecção de produto, vestuário e calçado. Entretanto, sugere-se aprimorar a etapa de engraxe do processo de curtimento.

This semestral paper deals with the issues of testing and requirements examinations for cables and cable harnesses. These terms are defined in the work and the difference in their meaning is described. The work also describes the characteristic features of cables and cable harnesses, explains their importance and focuses in more detail on the variable properties that may change over time or that are dependent on manufacturing errors. The principles and procedures of tests of these properties are covered in the work and are the essence of the subsequent practical part. In this part, the work deals with the design of the measuring circuit prototype for testing of the insulation resistance of cables and their desired continuity in bundles. This design is then physically implemented, the processes of designing and implementing are described in detail and finally its functionality is tested and presented to the reader.