Surface water pollution is prevalent in numerous areas of central Roodepoort mainly due to gold mining activities. The surface water quality for the Bosmontspruit, Russell’s Stream and the New Canada Dam was assessed from October 2010 to March 2011. Physical, chemical and biological characteristics of the water were determined for 8 monitoring points and the results obtained were compared with the In-stream water quality guidelines for the Klip River catchment and the South African Water Quality Guidelines. A trend noticed throughout the sampling period was the non-compliance in the levels of total dissolved solids (TDS) and dissolved oxygen. The results indicated that concentrations of iron, aluminium, nickel, manganese and potassium were above the limit across the Bosmontspruit and Russell’s stream. There was also significant evidence of excessive faecal coliform and ammonium pollution in the Bosmontspruit. During the monitoring period it was noted that water from these streams were utilised for crop irrigation, bathing, livestock and human consumption and may pose a health hazard due to poor water quality. / Environmental Sciences / M.Sc. (Environmental Science)

M.Sc. (Aquatic Health) / This study took place in the Groot Marico River catchment situated in the North West Province of South Africa. The catchment holds many rivers and associated tributaries that are home to a variety fish species with some being endemic to the catchment. A small minnow species, the Marico barb Barbus motebensis was chosen as the study species due to it being endemic to the catchment with limited information available on them with regards to their reproductive status in terms of their reproductive timing and number of potential offspring. Two tributaries from the upper Groot Marico River were chosen as sampling sites for the collection of B. motebensis across the four seasons. These two tributaries presented two genetically different populations, which allowed for a comparison of the findings to ascertain whether any differences in reproductive aspects occurred between the two tributary populations during the different seasons. The Marico barb is under threat from predation pressure posed by the largemouth bass Micropterus salmoides introduced into the system. Micropterus salmoides is a known alien invasive fish that eats any animal food item it encounters, especially fish. Due to B. motebensis and M. salmoides sharing the same habitat preferences, B. motebensis stands little chance of survival in the same water inhabited by the predatory M. salmoides. Many studies have shown bass to drive other fish species to the point of extirpation from a system through predation pressure. Currently B. motebensis is classified as vulnerable on the Red List of Threatened Species, although this status may be further compromised with the presence of M. salmoides...

In South Africa, precipitation is extremely variable and water is scarce. South Africa is also a country with great welfare needs. Challenging economic development targets and plans therefore need to be implemented successfully within the constraints of limited water supply and unreliable water availability. These economic development plans are underpinned by the development and growth of economic activities such as agriculture, mining, energy production and many types of small, medium and micro enterprises, which are some of the largest water using sectors in the economy. Within these activities, increased competition places pressure on water users to keep supplying their markets with competitively priced goods, while rising costs of new water supplies puts pressure on water users to allocate sufficient water to their production processes. These market forces and the relative scarcity of water as an economic production factor, impact on financial viability and imply that the economic efficiency of water use becomes increasingly important. The National Water Act of 1998 (NWA) is a legislative response to this situation, and promotes a radical shift towards efficiency and equity goals in water allocation. Water users who require water as an input to economic activities are consequently seriously revising their water use patterns in response to one of the major implications of the NWA and its related principal strategy: water demand management. Water demand management strives to adhere to the principles of equity, social justice, economic efficiency and environmental sustainability, which are central to the NWA. This study evaluates the costs and benefits of water use in order to simulate the effects of water demand management activities on a catchment economy. The results of a number of studies were combined to generate an economy-wide model: a Social Accounting Matrix (SAM), for the case study area and to simulate the direct and indirect effects of water demand management on the people, the economy and the natural environment in the area. Water demand management (WDM) is defined as consisting of two phases. In the first phase, goals of full cost recovery, improving water use efficiency and allocating water optimally are targeted. The second phase of WDM arrives when a situation of absolute water scarcity is reached within a catchment. In this phase water demand outweighs water supply and water has to be allocated according to its scarcity value. Water markets play a large role here. The SAM was used to simulate the direct and indirect impacts on the economy and the environment of a number of WDM related scenarios. Water e-allocation decisions and the effects of various WDM policy instruments, such as reduction of water use subsidies and increases in water tariffs were simulated. Unintended consequences of other environmental policies on water use, in this case, carbon tax, were explored. Water scarcity predictions were done, and some of the transaction costs involved in water trading was quantified. The study concludes with a discussion on the indirect effects on the economy, the environment and people of changes affecting the agricultural (including forestry) activities. The direct and indirect impacts of WDM policies on the economy and the environment, and the importance of environmental-economic models in water cost benefit modeling are also discussed. Implications for policy and management are highlighted. This study shows specifically how, through modelling various scenarios, policy decisions aimed at managing specific variables (e.g. water use, carbon emissions) have an economic and environmental impact much wider than the sector in which the policy was targeted for. Each scenario shows how a water transaction, or a change in subsidy in the agricultural (including forestry) sector, could impact on the output of other economic sectors, and therefore the economy as a whole. It is therefore evident that policy decisions, which are implemented at a macro level, and could have a major direct impact on a wider range of economic sectors, should be carefully considered as they could have large, undesirable, unintended consequences. / Dissertation (MSc (Agricultural Economics))--University of Pretoria, 2004. / Agricultural Economics, Extension and Rural Development / MSc (Agric) / unrestricted

Anthropogenic activities generate waste products that pollute the environment with bacteria and heavy metals. This research assessed pollution of the Kahwa River, Bukavu Town, DRC with cadmium and lead (HMs) and bacterial enteropathogens. A survey of businesses, households and healthcare facilities showed general use of the river to remove effluent and waste. Indicator organisms were cultured at over 200 cfu/100 ml showing faecal contamination of the river water. Antibiotic resistance was shown by enteropathogenic Vibrio cholerae and Salmonella typhi to ampicillin and cotrimoxazole with some sensitivity shown to ciprofloxacin. River water contained HMs at around 40 times the World Health Organisation limit for drinking water. The bacteria, particularly from river sediment, tolerated HMs up to a concentration of 1.5 mg/ml. The presence in the Kahwa River of antibiotic-resistant pathogens showing tolerance to HMs has serious public health implications / Environmental Management / M.Sc. (Environmental management)

M. Tech. (Biotechnology) Vaal University of Technology / One of the major problems encountered in qualitative and quantitative determination of residual pesticides by gas chromatography is the matrix effects. Matrix components have a considerable effect on the way analysis is conducted and the quality of results obtained, introducing problems such as inaccurate quantification, low analyte delectability and reporting of false positive or even false negative results. It was aimed to develop and validate a suitable method for counteracting the matrix effects so as to improve the detection and quantification of selected organochlorine pesticide residues from real water samples. The real water samples used were sampled from three points along the Jukskei River catchment area in Gauteng, South Africa for a period of 7 months from January to July 201 0 so as to create a representative sample. An automated solid phase extraction (SPE) method coupled to Gas ChromatographyMass Spectrometry (GC-MS) method for the analysis of 20 selected organochlorine pesticides was developed and validated for the purposes of studying the matrix effects. The analytical method showed a significant degree of validity when tested against parameters such as linearity, repeatability and sensitivity. Endosulphan beta, 4,4' Dichlorodiphenyldichloroethane, and Heptachlor-epoxide had the broadest linear calibration ranges of 1 ppm- 0.0156 ppm. Benzene hexachloride (BHC) delta and Lindane had the lowest statistical limits of detection of 0.018 ppm. Statistical hypothesis testing indicated that there was significant linearity in all selected organochlorine calibration curves. Four different reversed sorbent phases, including LC18, SC18- E and Strata-X (styrene divinyl benzene) were tested for organochlorine retention efficiency. The LC-18 200 mg cartridge proved to be the most robust and effective sorbent phase as it produced better recoveries varying from 90-130% for most analytes. A breakthrough volume of 100 ml for the LC-18 200 mg cartridge was determined using an optimum matrix load curve. It was then concluded that the method developed was suitable for further research towards the influence of the matrix on selective determination of the selected organochlorine pesticides. Four different calibration methods, namely matrix-free external standard, matrixmatched external standard, matrix-free internal standard and matrix-matched internal standard were applied to test the efficiency of computing recoveries. All calibration curves for the 20 organochlorine pesticides showed significant linearity > 0.99 when plotted on both Chemstation and Excel. The calibration methods were tested on three different matrices composed of a high sample matrix (synthetic matrix), a low sample matrix (real sample matrix) and a no sample matrix (ultrapure water). Statistical hypothesis testing led to the decision that there are significant differences between the mean recoveries of the three water sample matrices and also that the differences in the mean recoveries of the three sample matrices are independent of the both the two calibration techniques (internal standard and external standard) and calibration types (matrix-matched and matrix-free) applied. This led to the overall conclusion that the matrix effects have an overwhelming influence on the selective determination of the selected organochlorine pesticides.

Les bassins versants du pourtour méditerranéen français sont touchés par des pluies parfois intenses et à fort cumuls, qui peuvent engendrer des crues à cinétique rapide. Les derniers exemples majeurs en date sont ceux de l’Aude en 1999, du Gard en 2002 et du Var en 2010, dont les conséquences furent dramatiques. Ces travaux de thèse visent à évaluer une approche de modélisation complémentaire aux outils dont disposent déjà les Services de Prévision des Crues pour la prévision des crues à cinétique rapide : le couplage de modèles hydrologique et hydraulique, qui est a priori adapté pour la modélisation à l’échelle des grands bassins méditerranéens, de superficies supérieures à 1 000 km² (Ardèche, Cèze, Gardon, Vidourle…). Des éléments de réponses aux 4 questions suivantes sont recherchés : 1) le couplage est-il adapté pour la modélisation des débits atteints lors d’évènements passés, d’importance intermédiaire ? 2) le couplage est-il performant pour la modélisation des débits, cotes atteintes, et extension d’inondation, observés lors d’un épisode majeur? 3) comment envisager d’améliorer la modélisation des apports latéraux non-jaugés au modèle hydraulique, tout en adoptant une démarche adaptée à la prévision ? 4) le couplage est-il performant en prévision ? Le couplage employé combine le modèle hydrologique SCS-LR de la plateforme ATHYS (Bouvier et al., 2004), et le code de modélisation hydraulique 1D MASCARET (EDF-CETMEF, 2011). Il est appliqué au bassin versant du Gardon (2 040 km²), dans le sud de la France. / The French catchments around the Mediterranean Sea are affected by intense rains, which can cause fast and flash floods. The last major events are the one of the Aude river in 1999, of the Gard area in 2002, and of the Var area in 2010, whose consequences were tragic. This PhD intends to assess a modeling strategy complementary to the tools that are already used by the regional flood warning services: the coupling of hydrologic and hydraulic models, which is a priori well-adapted for the modelling of catchments of large-scale areas (larger than 1 000 km²) around the Mediterranean Sea (such as the ones of the Ardèche river, the Cèze river, the Vidourle river, the Gardon river…). The works aim at bringing elements of responses to the following questions: 1) is the coupling adapted to the modelling of floods hydrographs of past events of moderate importance? 2) in case of an extreme event (like in September 2002), is the coupling effective for the modelling of discharges, of water levels, and of flood extension? 3) how can we improve the modelling of ungauged lateral inflows to the hydraulic model, while applying a method adapted to forecasting? 4) Is the coupling efficient at forecasting? The coupling used combines the SCS-LR hydrologic model of the ATHYS platform (Bouvier et al., 2004), and the MASCARET 1D hydraulic model (EDF-CETMEF, 2011). It is applied to the Gardon river basin (2 040 km²), in the South of France.

Le but principal de cette thèse était d'étudier les caractéristiques de la matière organique dissoute (MOD) dans le bassin versant de la Seine. Ce travail a été réalisé dans le cadre du programme de recherche PIREN-Seine. Les travaux présentés ici visaient plus particulièrement à identifier les sources de MOD et à suivre son évolution dans les zones d’étude. L’analyse des propriétés optiques (UV-Visible, fluorescence) de la MOD, couplée aux traitements PARAFAC et ACP, a permis de discriminer différentes sources de MOD et de mettre en évidence des variations spatio-temporelles de ses propriétés. L’axe Seine, en aval de Paris, a notamment été caractérisé par l'activité biologique la plus forte. La MOD du bassin de l’Oise a montré des caractéristiques plus "humiques", tandis que le bassin de la Marne a été caractérisé par un troisième type spécifique de MOD. Il a d’autre part été mis en évidence la présence de MODs spécifiques dans chaque zone pour les échantillons prélevés en périodes d’étiage, alors qu’une distribution homogène des composants a été obtenue pour l’ensemble des échantillons prélevés en période de crue.Le rôle environnemental des colloïdes naturels étant étroitement lié à leur taille, il a d’autre part été développé une technique analytique/séparative originale pour l’étude de ce matériel complexe, un fractionnement par couplage flux/force avec flux asymétrique (AF4). Le fractionnement par AF4 des échantillons a confirmé la variabilité spatio-temporelle en composition et en taille de la MOD d'un site de prélèvement à un autre et a permis de distinguer différentes sources de MOD colloïdale confirmant les résultats de l’étude de ses propriétés optiques. / The main goal of this thesis was to investigate the characteristics of dissolvedorganic matter (DOM) within the Seine River catchment in the Northern part of France. ThisPhD thesis was performed within the framework of the PIREN-Seine research program. Theapplication of UV/visible absorbance and EEM fluorescence spectroscopy combined toPARAFAC and PCA analyses allowed us to identify different sources of DOM andhighlighted spatial and temporal variations of DOM properties. The Seine River wascharacterized by the strongest biological activity. DOM from the Oise basin seemed to havemore "humic" characteristics, while the Marne basin was characterized by a third specifictype of DOM. For samples collected during low-water periods, the distributions of the 7components determined by PARAFAC treatment varied between the studied sub-basins,highlighting different organic materials in each zone. A homogeneous distribution of thecomponents was obtained for the samples collected in period of flood.Then, a semi-quantitative asymmetrical flow field-flow fractionation (AF4) methodology wasdeveloped to fractionate DOM. The following optimized parameters were determined: across-flow rate of 2 ml min-1 during the focus step with a focusing time of 2 min and anexponential gradient of cross-flow from 3.5 to 0.2 ml min-1 during the elution step. Thefluorescence properties of various size-based fractions of DOM were evaluated by applyingthe optimized AF4 methodology to fractionate 13 samples, selected from the three sub-basins.The fluorescence properties of these fractions were analysed, allowing us to discriminatebetween the terrestrial or autochthonous origin of DOM.

Anthropogenic activities generate waste products that pollute the environment with bacteria and heavy metals. This research assessed pollution of the Kahwa River, Bukavu Town, DRC with cadmium and lead (HMs) and bacterial enteropathogens. A survey of businesses, households and healthcare facilities showed general use of the river to remove effluent and waste. Indicator organisms were cultured at over 200 cfu/100 ml showing faecal contamination of the river water. Antibiotic resistance was shown by enteropathogenic Vibrio cholerae and Salmonella typhi to ampicillin and cotrimoxazole with some sensitivity shown to ciprofloxacin. River water contained HMs at around 40 times the World Health Organisation limit for drinking water. The bacteria, particularly from river sediment, tolerated HMs up to a concentration of 1.5 mg/ml. The presence in the Kahwa River of antibiotic-resistant pathogens showing tolerance to HMs has serious public health implications / Environmental Management / M.Sc. (Environmental management)

>Magister Scientiae - MSc / The Kuils River Catchment is an urban river catchment that forms part of the larger Kuils-Eerste River system draining the eastern half of the Cape Metropolitan Authority area and Stellenbosch Municipality. Rapid urbanisation has resulted in the encroachment of residential and industrial areas into the river system through channelization and sewage disposal. This research project intends to assess the quality of surface runoff in the Kuils River catchment and determining non-point source pollutant loading rates in the catchment using GIS-based modelling. The study results show how modelled potential sources of surface runoff and NPS pollutants using desktop GIS analysis tools in a sequential process that involved different levels of software applications could explain the characteristics of the catchment. With the help of the Expected Mean Concentration (EMC) values associated with surface runoff from land use/covers, NPS pollutant loads were assessed downstream towards the Kuils River Catchment outlet using the Nonpoint Source Pollution and Erosion Comparison Tool (N-SPECT) based in ArcGIS. The outputs from this model consist of predicted annual pollutant loading (mg/mvyear) for each Kuils-Eerste River that occurs in the catchment. The results have shown clearly the spatial distribution of sources of particular pollutants in the catchment. Further or advanced processing knowhow with this model might provide far reaching insights into the problem and it is however recommended that these results produced using N-SPECT be compared to those of other hydrologic models using the same inputs.

Department of Hydrology and Water Resources / PhDH