<p>The past several years have witnessed a rapid development in the wireless network area. So far wireless networking has been focused on high-speed and long range applications. However, there are many wireless monitoring and control applications for industrial and home environments which require longer battery life, lower data rates and less complexity than those from existing standards. What the market need is a globally defined standard that meets the requirement for reliability, security, low power and low cost. For such wireless applications a new standard called ZigBee has been developed by the ZigBee Alliance based upon the IEEE 802.15.4 standard. </p><p>The aim of this diploma work is to design fully functional ZigBee and IEEE 802.15.4 modules, and to evaluate an application in a sensor network. </p><p>This diploma work has resulted in two fully functional ZigBee and IEEE 802.15.4 modules, respectively. It is also shown that ZigBee sensors can be networked wirelessly. Eventually it is the authors hope that the modules will be used within ITN, and also be developed further for new applications.</p>

The recommended procedures for implementing COTMAN; a cotton management expert system; suggest frequent crop scouting at numerous locations for each field. Machine based height sensors coupled with the ability to spatially record height values make it possible to locate regions of a field that are height representative of the entire field. A machine based height measurement system called HMAP was used to assess plant height in various fields in the 2003 growing season while the same fields were monitored with COTMAN. The plant height data was used to determine an optimal COTMAN sampling scheme for each field consisting of significantly fewer sampling locations than recommended by COTMAN. It was possible to ascertain equivalent information from COTMAN using two sites selected from height data in place of six sites selected per COTMAN recommendations. The HMAP system was extended to monitor rate of growth in real time in addition to plant height by comparing historical plant height data recorded on previous field passes to current height values. The rate of growth capable HMAP system will make it possible to track cotton growth and development with an automated system.

[N,N??-bis-(mercaptoethyl)-1,5-diazacyclooctanato]NiII, Ni-1, is known to undergo metallation reactions with numerous metals. [N,N??-bis-(mercaptoethyl)-1,5-diazacycloheptanato]NiII, (bme-dach)Ni or Ni-1??, differs from Ni-1 by one less carbon in its diazacycle backbone ring producing subtle differences in N2S2Ni geometry. Metallation of Ni-1?? with PdCl2, Pd(NO3)2, and NiBr2 produced three structural forms: Ni2Pd basket, Ni4Pd2 C4-paddlewheel, and Ni3 slant chair. In attempts to provide a rationale for the heterogeneity in the active site of Acetyl coA Synthase, metal ion capture studies of Ni-1 in methanol found a qualitative ranking of metal ion preference: Zn2+ < Ni2+ < Cu+. Formation constants for metal ion capture of Ni-1?? in water were determined for Pb2+, Ni2+, Zn2+, Cu+, and Ag+. A quantitative estimate places copper some 15 orders of magnitude above nickel or zinc in binding affinity. Sulfur dioxide uptake by Ni-1?? is characterized by significant color change, improved adduct solubility, and reversible binding of two equivalents of SO2. These combined properties establish Ni-1?? as a suitable model for gas uptake at nickel thiolate sites and as a possibly useful chemical sensor for this poisonous gas. Comparisons of molecular structures, ν(SO) stretching frequencies, and thermal gravimetric analyses are made to reported adducts including the diazacyclooctane derivative, Ni-1·2SO2. Visual SO2 detection limits of Ni-1 and Ni-1?? are established at 25 ppm and 100 ppm, respectively. Structural studies of products resulting from reaction at the nucleophilic S-sites of (bme-dach)Ni and [(bme-dach)Zn]2 included acetyl chloride and sodium iodoacetate as electrophiles are shown. The acetyl group is a natural electrophile important to the citric acid cycle. Acetylation of (bme-dach)Ni produces a five coordinate, paramagnetic species. Iodoacetate is a cysteine modification agent known to inhibit enzymatic activity. The reaction of (bme-dach)Ni and sodium iodoacetate yields a blue, six coordinate nickel complex in a N2S2O2 donor environment. The bismercaptodiazacycloheptane ligand binds lead(II) forming an unprecedented structural form of N2S2M dimers, in which Pb2+ is largely bound to sulfur in a highly distorted trigonal geometry. Its unusual structure is described in comparison to other derivatives of the bme-daco ligand. The synthesis and structural characterization of square pyramidal (bme-dach)GaCl are also given and compared to the analogous (bme-daco)GaCl.

Methods to improve performance of sensors with regard to sensor nonlinearity, sensor noise and sensor bandwidths are investigated and new algorithms are developed. The necessity of the proposed research has evolved from the ever-increasing need for greater precision and improved reliability in sensor measurements. After describing the current state of the art of sensor related issues like nonlinearity and bandwidth, research goals are set to create a new trend on the usage of sensors. We begin the investigation with a detailed distortion analysis of nonlinear sensors. A need for efficient distortion compensation procedures is further justified by showing how a slight deviation from the linearity assumption leads to a very severe distortion in time and in frequency domains. It is argued that with a suitable distortion compensation technique the danger of having an infinite bandwidth nonlinear sensory operation, which is dictated by nonlinear distortion, can be avoided. Several distortion compensation techniques are developed and their performance is validated by simulation and experimental results. Like any other model-based technique, modeling errors or model uncertainty affects performance of the proposed scheme, this leads to the innovation of robust signal reconstruction. A treatment for this problem is given and a novel technique, which uses a nominal model instead of an accurate model and produces the results that are robust to model uncertainty, is developed. The means to attain a high operating bandwidth are developed by utilizing several low bandwidth pass-band sensors. It is pointed out that instead of using a single sensor to measure a high bandwidth signal, there are many advantages of using an array of several pass-band sensors. Having shown that employment of sensor arrays is an economic incentive and practical, several multi-sensor fusion schemes are developed to facilitate their implementation. Another aspect of this dissertation is to develop means to deal with outliers in sensor measurements. As fault sensor data detection is an essential element of multi-sensor network implementation, which is used to improve system reliability and robustness, several sensor scheduling configurations are derived to identify and to remove outliers.

Le domaine des senseurs chimiques et biochimiques est en pleine expansion, tant au niveau de la recherche fondamentale que du design et de la fabrication de nouveaux « nez artificiels ». Les recherches actuelles visent à la mise au point de dispositifs de taille réduite permettant l’identification et la quantification d’espèces multiples, avec une réponse rapide et réversible, une détection sensible et sélective, le tout intégré dans une technologie simple et peu coûteuse. Les composantes actives des nez artificiels dont il est question ici reposent sur l’utilisation en tant que composante active de polymères et molécules conjugués. Les polymères conjugués sont attractifs pour leur grande sensibilité (tributaire du processus de diffusion efficace des excitations électroniques le long des chaînes) tandis que les molécules conjuguées répondent généralement favorablement au cahier des charges imposé à un senseur, dont les interactions fortes et spécifiques entre le senseur et la molécule à détecter ou encore une grande stabilité chimique et photochimique. Dans ce contexte, le but de cette thèse est d’associer polymères et molécules conjugués pour mettre au point un dispositif hybride combinant les avantages des deux composantes. Afin de caractériser ces dispositifs et de les optimiser, nous nous sommes focalisés dans un premier temps sur l’étude des propriétés géométriques, électroniques et optiques des entités séparées, que nous avons étudiées par le biais de méthodes issues de la chimie quantique. D’une part, nous avons considéré les améliorations pouvant être apportées aux détecteurs moléculaires tant au niveau des limites de détection que de ses propriétés spectroscopiques. D’autre part, nous avons mené une étude mécanistique des processus de transfert d’énergie le long de chaînes de polymères conjugués rigides, en apportant une attention particulière sur les différentes approches envisageables pour en améliorer l’efficacité (effets conformationnels, introduction d’unités à longues durées de vie, écart énergétique donneur-accepteur). Enfin, sur base des résultats obtenus pour les constituants séparés, nous avons proposé des structures chimiques de systèmes ‘hybrides’, constitués de polymères conjugués substitués en bouts de chaîne par des groupements ionophores. Ces études ont été menées en étroite collaboration avec le groupe du Prof. Noël Boens à la KULeuven, où les molécules et polymères conjuguées étudiés théoriquement dans le cadre de ce travail ont été synthétisés et caractérisés d’un point de vue spectroscopique.

Wilting during shelf life is a major cause of postharvest shrink for bedding plants shipped long distances from production greenhouses to retail outlets. The objective of this research was to determine if irrigation at lower, constant substrate moisture content (SMC) during greenhouse production would be a feasible way to acclimate plants for reduced shrinkage during shelf life, while potentially conserving irrigation water. Rooted plugs of Angelonia angustifolia 'Angelface Blue' were grown in greenhouse production until a marketable stage in substrates irrigated at SMC levels of 10, 20, 30, and 40% using a controlled irrigation system. At the end of the greenhouse production stage, plants were irrigated to container capacity and subjected to a simulated shipping environment, in shipping boxes in the dark for two days. After shipping, plants were placed back in the greenhouse and watered minimally to simulate a retail environment. Data was taken at the end of each stage i.e. greenhouse production, simulated shipping, and simulated retail. Parameters measured at the end of the production stage were fresh and senesced flower number, stem number, pre-dawn and mid-day water potential, SPAD meter readings (Experiment 2), and plant height and node number segmented into vegetative, flowering, and bud area. Plant quality was observed and rated. At the end of the simulated retail stage, the same data was taken, along with fresh and dry shoot and root weight. Results indicated that as SMC decreased from 40 to 10%, plants were shorter in height, but had proportional flowering sections (Experiment 1) or more compact flowering sections (Experiment 2). The volume of water received by the 40% SMC plants was three times greater (Experiment 1) and 12 times greater (Experiment 2) than the 20% SMC plants during greenhouse production, and two times greater (Experiment 1) and nine time greater (Experiment 2) during simulated retail. Additionally, the 40% SMC plants used 15 liters (Experiment 1) and 38 liters (Experiment 2) of water during greenhouse production compared to the 20% SMC plants using only three liters in both experiments. During simulated retail the 40% SMC plants used six liters (Experiment 1) and nine liters (Experiment 2) of water while the 20% SMC plants used five liters (Experiment 1) and three liters (Experiment 2) of water. During production, mid-day water potentials decreased as the SMC levels decreased, but at the end of the simulated retail (Experiment 1), the mid-day water potentials were all the same, suggesting plants that were drought stressed during production area were acclimated to lower water levels experienced in retail settings. Overall, the 20% SMC treatment produced the best postharvest quality plant due to reduced plant height without detrimental effects on flowering. The results demonstrate that while conserving water, controlled irrigation at a medium-low SMC can produce high quality plants that have equal shelf life to those that are irrigated at high levels.

Diabetes mellitus affects 25.8 million Americans (8.3%) and over 300 million people worldwide. Clinical trials indicate that proper management of blood glucose levels is critical in preventing or delaying complications associated with diabetes. Thus, there is a common need to monitor and manage blood glucose properly for people with diabetes. However, the patients’ compliance for recommended monitoring frequency is low due to the pain and inconvenience of current standard finger-pricking tests. To promote patient adherence to the recommended self-monitoring frequency, non-invasive/ minimally invasive glucose testing approaches are needed. Luminescent microparticle sensor is an attractive solution. For these sensors to be deployed in vivo, a matched optical system is needed to interrogate dermally-implanted sensors. This research project investigated the light propagation in skin and the interaction with implants using Monte Carlo modeling. The results of the modeling were used to design an optical system with high interrogation and collection efficiency (40~300 times improvement). The optical system was then constructed and evaluated experimentally. A stable skin phantom mimicking the optical properties of human skin was developed as a permanent evaluation medium to minimize the use of animals. The optical properties of the skin phantom matched the maximum published values of human skin in scattering and absorption over the spectral range of 540~700nm in order to avoid overestimation of the capability of the system. The significant photon loss observed at the connection between the designed system and a commercial spectrometer was overcome using two optimized designs: a two-detector system and a customized low-resolution spectrometer system. Both optimization approaches effectively address the photon loss problem and each showed good SNR (>100) while maintaining a sufficient system resolution for use with fluorescent materials. Both systems are suitable for luminescence measurement, because broad bands of the luminescent spectrum are of interest. In the future, either system can be easily modified into a more compact system (e.g. handheld), and it can be directly coupled to an analog-to-digital converter and integrated circuits offering potential for a single compact and portable device for field use with luminescent diagnostic systems as well as implanted sensors.

<p>Fluorescent sensors provide a powerful analytical tool for the intracellular detection of metal cations. In some cases, these fluorescent metal-chelating sensors have helped elucidate the function of metal cations within complicated cellular systems. However, most measure or sense changes in the bulk concentration of a metal species and do not respond to those involved in a specific cellular event. For instance, misregulated copper and iron are implicated in neurodegenerative disease and cancer because of their ability to catalytically propagate the formation of the hydroxyl radical through reaction with hydrogen peroxide. A fluorescent sensor that is unresponsive to metal binding until activation by intracellular hydrogen peroxide could potentially pinpoint the location of this oxidative reaction and provide an understanding of the relationship between copper/iron and hydrogen peroxide. </p><p> Described here is the development of two fluorescent prochelators that show a selective fluorescence response to iron or copper only in the presence of hydrogen peroxide. A boronic ester masked spirolactam-based prochelator displays a copper-selective turn-on response after oxidation with hydrogen peroxide in organic solvents as determined by absorbance and fluorescence spectroscopy. However, a competing mechanism occurs in aqueous solution due to hydrolytic instability of the masked prochelator and results in a separate copper-dependent turn-on response as verified by liquid chromatography-mass spectroscopy. A second fluorescent prochelator design relies on metal-dependent fluorescence quenching after oxidation of a self-immolative boronic ester in both organic and aqueous solvents. Cellular microscopy studies show that the sensor's fluorescence intensity is unchanged until incubation with exogenous hydrogen peroxide, which resulted in a decreased fluorescent signal that is restored upon competitive chelation. Both of these prochelators provide a template for future applications and designs with improved properties.</p><p> Two additional chapters describe the development of a UV-activated iron prochelator and a new fluorescently tagged metal chelator. The UV-activated prochelator is protected with two nitrophenyl groups that are photolyzed with 350 nm light within 10 minutes to reveal a high affinity iron triazole-base chelator. A chelator of this nature may provide protection from UV-induced iron liberation and oxidative stress. A second triazole-based chelator with an embedded coumarin fluorophore was prepared as a potential metal sensor. However, this design showed off-target fluorescence responses, thus it cannot be utilized in its current form for metal detection.</p> / Dissertation

Current municipal solid waste (MSW) practices have encouraged rapid waste degradation (stabilization) as an alternative to past methods of isolating the waste from the surrounding environment. There are challenges to rapid-stabilization technology, in particular, the management of the in-situ MSW moisture content.<p> The primary objective of this study was to evaluate the use of capacitance moisture probes for the purpose of measuring the moisture content within MSW. Capacitance moisture probes have not previously been used in MSW, however their use in agriculture is extensive and knowledge of their potential for monitoring MSW is limited.<p> The specific objectives of this research were to: i) establish a laboratory based correlation between sensor data and volumetric moisture content in MSW, ii) establish a correlation between field-installed capacitance sensors and moisture content derived from continuous-depth in-situ sampling of MSW, and iii) demonstrate the ability of capturing advancing/receding moisture fronts with the field-installed capacitance sensors.<p> Laboratory trials were conducted using hand-compacted MSW at volumetric moisture contents ranging from 15%-55% and a manual type of capacitance sensor. This series of laboratory trials successfully produced a correlation between sensor output and volumetric moisture content.<p> To evaluate the sensors in a real-world application, two configurations of capacitance moisture probes were installed in the field: i) an in-place, continuous-time capacitance probe, and ii) a portable, continuous-depth at discrete time, capacitance probe.<p> Field results indicated that capacitance moisture probes were able to capture the passing of both an artificially and naturally induced moisture front, though quantitative correlation between the in-situ moisture content of the sampled MSW and the readings of the sensors could not be achieved.<p> The reasons for this were a combination of three factors:<p> 1. The introduction of void-space during sensor installation significantly reduced sensor output;<p> 2. Poor MSW sampling technique resulted in 57% recovery (causing the exact origin of samples to be unknown); and<p> 3. The sampling technique disturbed the MSW samples, resulting in incorrect volumetric moisture contents in the samples.

Near-fall detection is important for medical research since it can help doctors diagnose fall-related diseases and also help alert both doctors and patients of possible falls. However, in people’s daily life, there are lots of similarities between near-falls and other Activities of Daily Living (ADLs), which makes near-falls particularly difficult to detect. In order to find the subtle difference between ADLs and near-fall and accurately identify the latter, the movement of whole human body needs to be captured and displayed by a computer generated avatar. In this thesis, a wireless inertial motion capture system consisting of a central control host and ten sensor nodes is used to capture human body movements. Each of the ten sensor nodes in the system has a tri-axis accelerometer and a tri-axis gyroscope. They are attached to separate locations of a human body to record both angular and acceleration data with which body movements can be captured by applying Euler angle based algorithms, specifically, single rotation order algorithm and the optimal rotation order algorithm. According to the experiment results of capturing ten ADLs, both the single rotation order algorithm and the optimal rotation order algorithm can track normal human body movements without significantly distortion and the latter shows higher accuracy and lower data shifting. Compared to previous inertial systems with magnetometers, this system reduces hardware complexity and software computation while ensures a reasonable accuracy in capturing human body movements.