Wir berichten über eine experimentelle Studie zu lokalen Suchverfahren für das Pairing-Problem bei Fluggesellschaften unter Benutzung realer Testdatensätze mit bis zu 4600 Fl"ugen kurzer Reichweite. Unter einer Run-Cutting Formulierung vergleichen wir Verfahren wie Simulated Annealing, Modifikationen der Wasserspiegel-Algorithmen nach Dueck, eine elementare Implementation von Tabu Suche sowie einen GRASP Ansatz. Es zeigt sich, da"s die Wasserspiegel-Algorithmen vergleichbare Ergebnisse wie Simulated Annealing, und um etwa 40% bessere Ergebnisse als blo"se lokale Verbesserungsheuristiken liefern, w"ahrend Tabu Suche und GRASP hier ungeeignet erscheinen. Die Ergebnisse von Simulated Annealing und der Wasserspiegel-Algorithmen k"onnen sich auch mit den LP-Schranken f"ur die SET PARTITIONING Formulierung mit Packungsungleichungen und bis zu 3.9 Millionen Spalten messen. Eine Clustering-Parallelisierung des Simulated Annealings auf einem Parallelrechner GigaCluster PowerPlus weist eine sehr gute Beschleunigung auf. / For the airline pairing problem using a run-cutting formulation. Computational results are reported for some real-world short-haul testproblems with up to 4600 flights per month. In particular, we evaluate the relative performance of simulated annealing, modified versions of the waterlevel algorithms proposed by Dueck, an elementary tabu search, and a GRASP approach. We find that the waterlevel algorithms compare well with simulated annealing and both improve on mere improvement heuristics by about 40%. Tabu search and GRASP do not yield competitive results here. Simulated annealing and the waterlevel algorithms also compare favourably with LP-bounds for a SET PARTITIONING formulation with knapsack constraints containing up to 3.9 million columns. A clustering parallelization of simulated annealing on a GigaCluster PowerPlus parallel computer exhibits an excellent speed-up.

In dieser Arbeit werden zwei parametrische Aufgaben untersucht, Optimierungsprobleme und Variationsungleichungen. Beide Probleme werden unter der urspruenglich fuer einparametrische Optimierungsprobleme definierten Regularitaet im Sinne von Jongen, Jonker und Twilt betrachtet. Unter der oben genannten Regularitaet bezueglich Optimierungsproblemen werden zwei spezielle Einbettungen studiert. Ausgehend von der Kenntnis eines inneren Punktes des zulaessigen Bereiches eines Optimierungsproblems P wird eine Einbettung definiert, deren parametrische zulaessige Menge fuer t<1 in Inneren von P ist. Fuer t=0 ist die Loesung trivial und fuer t=1 wird das Problem P erzeugt. Die Erreichbarkeit des Parameterwertes t=1 bei der Benutzung von Kurvenverfolgungsverfahren mit Spruengen wird eroertert. Durch die Unterscheidung der verschiedenen Richtungen in t, in denen ein Rueckkehrpunkt auftreten kann, wird bewiesen, dass die genannte Methode erfolgreich ist (bei erfolgreichen Spruengen). Fuer diese Einbettung wird die Voraussetzung der Regularitaet untersucht und ein Rechtfertigungssatz bewiesen. Durchgefuehrt wird ein kurzer Vergleich mit einer aehnlichen Homotopie in der Literatur. Fuer die zweite untersuchte Einbettung (eine Penalty-Einbettung aus der Literatur) wird ein Satz zur Rechtfertigung der Regularitaetsvoraussetzung bewiesen. Die Regularitaet im Sinne von Jongen, Jonker und Twilt wird fuer den Fall von Variationsungleichungen definiert und die 5 Typen neu beschrieben. Die Unterschiede zu den Typen bei Optimierungsproblemen ergeben sich aus der Tatsache, dass die Symmetrie der Hessematrix der Lagrangefunktion bezueglich x verloren geht. Das erfordert die sorfaeltige Umformulierung einiger Bedingungen. Auss erdem musste bei den Beweisen der lokalen Eigenschaften (geometrische Struktur, Aenderung der charakteristischen Groessen) diese neue Situation beruecksichtigt werden. Ebenfalls wird der generische Charakter dieser Regularitaet nachgewiesen. Aussagen ueber die Existenz verbindender Kurven zwischen t=0 und t=1 unter der Voraussetzung der Regularitaet werden angefuehrt. Die gesamte Betrachtung der genannten Regularitaet fuer einparametrische Variationsungleichungen und die daraus resultierenden Schluss folgerungen liefern einen nuetzlichen Beitrag zur Untersuchung von Variationsungleichungen. / We consider two types of one-parametric problems: optimization problems and variational inequalities. Both are studied in relationship with the regularity approach due to Jongen, Jonker and Twilt (JJT-regularity), which was defined initially only for one-parametric optimization problems. The properties of two special embeddings are analysed under the assumption of JJT-regularity. Given an optimization problem P, the first embedding analysed is defined with use of an interior point of the feasible set. It holds for this embedding, that the parametric feasible set for tglobal approach. Statements concerning the existence of solution curves connecting problems (for instance t=0 and t=1) under the defined regularity are studied in the last section. The consideration of JJT-regularity for the case of variational inequalities is a new and usefull aspect for the study of this problem.

Diese Arbeit ist motiviert durch das allgemeine Interesse an den Modulräumen von basierten SU(r)-Instantonen auf der vierdimensionalen Sphäre und auf der zusammenhängenden Summe von komplexen projektiven Ebenen, welche als Modulräume von gerahmten holomorphen Vektorbündeln auf Aufblasungen von Regelflächen interpretiert werden können. Genauer gesagt, betrachten wir Modulräume von basierten SU(r)-Instantonen auf all denjenigen selbstdualen vierdimensionalen Mannigfaltigkeiten, welche eine Fläche vom Grad 1 enthalten, die selbst wiederum eine Twistorfaser enthält. Dies trifft zum Beispiel für die wohluntersuchte Klasse von LeBrun-Twistorräumen zu. Es erfolgt eine Darstellung des notwendigen Hintergrundes dieses Zusammenhanges. Inspiriert durch Hurtubise's Artikel "Instantons and Jumping Lines" untersuchen wir das lokale Sprungverhalten dieser gerahmten Vektorbündel und führen den Begriff des gerahmten exzeptionellen lokalen Sprunges ein. Wir beschreiben die gerahmten gewöhnlichen und exzeptionellen Sprünge durch Monaden und untersuchen die geometrischen Eigenschaften ihrer feinen Modulräume. Aufbauend auf dieser Untersuchung und den Resultaten von Boyer, Hurtubise, Mann, Milgram und Tian, worin die Atiyah-Jones-Vermutung für basierte SU(r)-Instantonen auf der vierdimensionalen Sphäre bewiesen wird, zeigen wir homologische und homotopische Charge-Stabilität für alle betrachteten Modulräume basierter SU(r)-Instantonen. Darüberhinaus stellen wir eine glatte Kompaktifizierung dieser Modulräume vor. / This work is motivated by the general interest in moduli spaces of based SU(r)-instantons on the four dimensional sphere and on the connected sum of complex projective planes, which can be interpreted as moduli spaces of framed holomorphic vector bundles on blown up ruled surfaces. To be precise, we consider moduli of based SU(r)-instantons over all self-dual four dimensional manifolds, where the twistor fibration contains a surface of degree 1 which itself contains a twistor fibre. This applies for instance to the well-examined class of LeBrun-twistor spaces. We display the necessary background of this relationship. Inspired by Hurtubise's paper "Instantons and Jumping Lines", we study the local jumping behaviour of such framed vector bundles and introduce the concept of framed exceptional local jumps. We describe framed ordinary and exceptional jumps by monads and examine the geometric properties of their fine moduli spaces. Based on this examination and on the results of Boyer, Hurtubise, Mann, Milgram and Tian, where the Atiyah-Jones conjecture for based SU(r)-instantons on the four dimensional sphere is proved, we show homological and homotopical charge stability for all considered moduli of based SU(r)-instantons. Moreover, we present a smooth compactification of these moduli spaces.

The objective was to study apoptotic effects and underlying molecular mechanisms of goniothalamin (GTN) in hepatocellular carcinoma (HCC)-derived cells. The GTN that isolated from Goniothalamus amuyon, was found to possess profound cytotoxic activities against human SK-Hep1 and Hep-3B cells in vitro. The cytotoxicity of GTN/cell viability was measured by MTT assay at 570-nm absorbance and the IC50 at 72 h were determined as 7.5, 17 mM in SK-Hep1 and Hep-3B respectively. The GTN induced cell death and accumulation of reactive oxygen species in HCC-derived cells. One reactive oxygen species inhibitor, N-actylcysteine, further restored cell viabilities post-GTN treatments. Formation of £^-H2AX foci suggested that GTN-induced DNA damages were double-strand breaks. The GTN arrested cell cycle at G0/G1 by regulation of CCND, CCNE1, RB1 and E2F1 proteins in SK-Hep1, and at G2/M by regulation of CCNB1 in Hep-3B cells. The GTN-induced apoptosis in HCC-derived cells were evidenced by phosphatidylserine externalization and involved both extrinsic and intrinsic pathways in SK-Hep1 cells, but only extrinsic pathway in Hep-3B cells. In SK-Hep1 cells, GTN induced apoptosis and cell cycle arrest through TP53-mediated pathway in contrast to that of TP53/FAS mutated Hep-3B cells. Importantly, GTN was able to induce apoptosis in both TP53 wild type and TP53/FAS mutated HCC-derived cells. On the other hand, GTN was able to induce TP53 and p21WAF1/Cip1 up-regulation in SK-Hep1 and Hep-3B cells and p27Kip1 up-regulation in Hep-3B cells. These results demonstrated that GTN induced apoptosis in HCC cells through distinct signaling pathways.

The SK 60 Aircraft has been a workhorse for the Air Force’s training of future pilots since the late 1960s. The aging SK60 aircraft is going out of style, and the system has merely a few years of total running time left. A replacement for the SK 60 needs to be implemented in the coming years, and the Armes Forces Flying School has been commissioned to conduct flight tests on suitable replacement systems. This study will conduct a system analysis of two aircrafts mentioned in the Flying Schools report. The systems are mainly valued on their suitability as a replacement for the SK 60 from a training perspective. The systems are also valued based on the possibility of expanding the military utility of each system such as their ability to carry out attack operations. Based on the sample the Pilatus Aircraft PC -21 and BAE Systems Hawk MK 120 aircrafts were chosen for evaluation. The two planes are different yet comparable which makes them suitable for comparison in this study. Hawk MK 120 was deemed as the most suitable replacement for the SK 60. The MK 120 appeared as slightly more favorable than the PC-21 after valuation and appraisal, both from an educational point of view but also with regard to its ability for extended military purposes.

The aim of this thesis is to identify and analyse factors affecting the attendance of domestic football matches of SK Dynamo club České Budějovice. The main emphasis is placed on working with fans. The theoretical part relates to the topic and focuses on the determining factors of demand for sport events, the sport product theory and phenomenon of a fan. The practical part introduces the club SK Dynamo České Budějovice more closely and analyses individual factors affecting demand for the club's matches and defines the significance of these factors. A chapter on working with the fans follows. At first the elements of sport product are characterised and secondly the club marketing elements. The practical section chapters are underpinned by the results of a survey which was carried out among the fans of SK Dynamo České Budějovice. The discourse and conclusion summarize the analysed results, evaluates working with fans and last but not least outlines possible steps for improvements of attendance and working with club's fans.

Master´s thesis is analyzing egual alternatives of women in term of business activities also macroeconomic indicators, which can zoom in situation on labour market. It represents acquisition for the future at the hand of specific arrangements.

No description available.

Thesis name Strategic Development Plan of the SK Joudrs Prague Thesis objective The aim of this diploma thesis is to draw up a strategic plan for the development of Joudrs Prague softball team for the period form 1st October 2015 to 30th September 2018. Methods of work A qualitative interview with the club President, a qualitative interview with the sports manager of the club, an analysis of texts and documents, the SWOT analysis, monitoring, descriptive case study Results An analytic part of the diploma thesis was built on the descriptive case study as well as on monitoring which provided an insight into the internal environment of the club and revealed the opinions of SK Joudrs Prague club members. The included SWOT analysis was developed on the basis of interviews and the dependence among the most important points was summarised in the table. The choice of the strategy resulted from the SWOT analysis. The financial analysis of the club was set up with regard to the interview with the club President and relevant documents. Key words Strategic planning, strategic plan, sports club, SK Joudrs Prague, softball

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No. of bitstreams: 4 Tese - Débora Cristina Silva dos Passos - 2013 - Parte 01.pdf: 10357491 bytes, checksum: 1678b4aad93bca319ee5ddb93d3808bc (MD5) Tese - Débora Cristina Silva dos Passos - 2013 - Parte 02.pdf: 9533486 bytes, checksum: ad5e21870e09f4f46a3045a69996aed3 (MD5) Tese - Débora Cristina Silva dos Passos - 2013 - Parte 03.pdf: 8695341 bytes, checksum: 5f8d696c0a73f923cb129f3c0aa1e4b7 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-05-16 / Melanoma is a type of cancer that arises from melanocytes and is notoriously resistant to radiation and chemotherapy. The thiosemicarbazones are synthetic compounds with marked biological properties such as antibacterial, antiviral, antiprotozoal and antitumor and previous studies have demonstrated cytotoxic activity against the human melanoma cells, so in this study, we evaluated the antiproliferative activity, the enzymatic activity of Caspases 2, 3, 6, 8, 9, the effect on the cell cycle gene expression levels of caspases 2, 3, 6, 8, 9, Apaf-1 and microscopic morphological changes in human melanoma cells (SK -MEL-37) twenty one monoterpene derived from natural thiosemicarbazone (-) - camphene: camphene, benzaldehyde, benzophenone, menthone, ethyl pyruvate, p-nitroacetophenone, pchloroacetophenone, p-methoxyacetophenone, p-methylacetophenone, p fluoracetofenona-phidroxiacetofena, furan, 3-methoxy-4-hydroxybenzaldehyde, p-fluorbenzaldehyde, 2- hydroxybenzaldehyde, cinnamic aldehyde, thiophene-2-carboxaldehyde, 1-H-imidazole-4- carboxaldehyde, tiossemicaroazida and six montoterpeno natural R-(+)-limonene: benzaldehyde, thiosemicarbazide, o-nitro, m-nitro, p-nitro, p-hydroxy and p-dimethylamino. The values found for the inhibitory concentration for 50% of cells (IC50) were between 12 μM and 55 μM. The percentage of cells in phase and in phase G0/G1 decreased SG2 / M increased after forty-eight hours of incubation with benzaldehyde thio-camphene, limonene thio-benzaldehyde, m-nitro, p-hydroxy and thiosemicarbazide increased indicating that the growth inhibitory effect might be also due to arrest of cells at S-G2/M phase. We observed increased activity of caspase 3 (m-nitro thio-limonene), 6 (camphene thio-benzaldehyde and p-hydroxy thio-limonene) and 8 (thio-benzaldehyde limonene). Late apoptotic features were detected in 62% of cells treated with benzaldehyde thio-camphene and morphological changes typical of apoptosis were visualized by fluorescence microscopy and scanning electron microscopy (SEM) after treatment with benzaldehyde thio-camphene chosen due to their low IC50 value (12 mM). It was observed gene expression of caspases 2, 3, 6, 8 and Apaf-1 in cells treated with benzaldehyde thio-camphene indicating the participation of these enzymes in the anti-proliferative effect observed. Our results indicate that the thiosemicarbazones derivatives can inhibit proliferation, regulate cell cycle, induce apoptosis of human melanoma cells (SK-MEL-37) and could be an candidate for future preclinical in vivo studies. / O melanoma é um tipo de câncer que surge nos melanócitos e é notoriamente resistente à radioterapia e quimioterapia. As tiossemicarbazonas são compostos sintéticos com marcantes propriedades biológicas tais como antibacteriana, antiviral, antiprotozoária e antitumoral e em estudos anteriores demonstraram ação citotóxica frente à celulas de melanoma humano, por isso, neste estudo, foi avaliada a atividade anti-proliferativa, a atividade enzimática das caspases 2, 3, 6, 8, 9, o efeito no ciclo celular, os níveis de expressão gênica das caspases 2, 3, 6, 8, 9, Apaf-1 e as alterações morfológicas por microscopia em células de melanoma humano (SK-MEL-37) de vinte e uma tiossemicarbazonas derivadas do monoterpeno natural (-)- canfeno: canfeno, benzaldeído, benzofenona, mentona, etil piruvato, acetofenona, pnitroacetofenona, p-cloroacetofenona, p-metoxiacetofenona, p-metilacetofenona, pfluoracetofenona, p-hidroxiacetofena, furano, 3-metóxi-4-hidroxibenzaldeído, pfluorbenzaldeído, 2-hidroxibenzaldeído, aldeído cinâmico, tiofeno-2-carboxialdeído, 1-Himidazol- 4-carboxialdeído, tiossemicaroazida, bem como seis do montoterpeno natural R-(+)- limoneno: benzaldeído, tiossemicarbazida, o-nitro, m-nitro, p-nitro, p-dimetilamino e phidróxi . Os valores encontrados para a concentração inibitória para 50% das células (IC50) situaram-se entre 12 μM e 55 μM. A porcentagem de células na fase G0/G1diminuiu e na fase SG2/M aumentou após quarenta e oito horas de incubação com o benzaldeído tio-canfeno, benzaldeído tio-limoneno, m-nitro, p-hidróxi e tiossemicarbazida, indicando que o efeito antiproliferativo observado pode ser devido a uma interrupção das células na fase SG2/M. Observou-se uma maior atividade de caspase 3 (m-nitro tio-limoneno), 6 (benzaldeído tiocanfeno e p-hidróxi tio-limoneno) e 8 (benzaldeído tio-limoneno). Características apoptóticas tardias foram detectados em 62% das células tratadas com benzaldeído tio-canfeno e as alterações morfológicas típicas de processo de apoptose foram visualizadas através da microscopia de fluorescência e de microscopia eletrônica de varredura (MEV) após tratamento com o benzaldeído tio-canfeno escolhido devido ao seu baixo valor de IC50 (12 μM). Observou-se a expressão gênica das caspases 2, 3, 6, 8 e o apaf-1 nas células tratadas com benzaldeído tio-canfeno indicando a participação dessas enzimas no efeito antiproliferativo observado. Os resultados indicam que as tiossemicarbazonas derivadas de canfeno e limoneno podem inibir a proliferação celular, regular o ciclo celular e induzir apoptose nas células de melanoma humano (SK-MEL-37), portanto, podem ser considerados candidatos para futuros ensaios pré-clínico in vivo.