The master thesis is focused on mapping of all the processes that customer goes through before achieving the skiing activity in ski resort Špindlerův Mlýn, currently operated by MELIDA, a.s. In the theoretical part services specifications, Six Sigma methodology and Key Performance Indicators basics are briefly described. The practical part consists of introduction of the ski area, measuring and mapping processes in combination with weak points identification. In order to eliminate weak points previously identified, the process of getting feedbacks from customers was introduced. As a result of this process, actions to eliminate the weak spots, were proposed and will be implemented. In order to maintain the appropriate structure of the thesis, Six Sigma methodology procedures and instruments, were used.

The proposed VUT Art Campus is situated around the area of the streets Úvoz, Údolní, Tvrdého, Všetičkova and Jiříkovského. It lies in between two of the Brno’s most attractive recreational spots, Špilberk and Kraví hora. The project offers a creative solution of connecting two VUT’s faculties, the Faculty of Architecture and the Faculty of Fine Arts. An important part of the proposal is the gallery of the students’ works which also serves as a connecting element between the faculties. One of the main objectives is the creation of the Green Line originating at Špilberk and finishing at Kraví hora. As such, the campus works as a cultural point of interest on the way around the city. The whole concept aims to highlight the uniqueness of the faculties while stretching the importance of their interconnection and the need of a co-operation.

Background: The whole of society is facing major challenges regarding the ongoing climate change and the possibility of creating sustainable development. This also largely applies to today's trafficsystem, which needs to change to more sustainable trffic system. Increased active travel is a nescessery for reaching the set goals that exist when it comes to sustainable travel. Increased active travel will lead to more unprotected road users in the trafficsystem, one group of unprotected road users are pedestrians. An increased number of pedestrians in the trafficsystem requires additional efforts to ensure traffic safety for that group. Method: An scoping review is carried out in which police and legislative measures aimed at increasing road safety for pedestrians are evaluated. The purpose of the study is to gain knowledge about what measures the current research proposes to increase traffic safety and to come up with proposals for additional areas where more research is needed. The study builds on two previous literature studies that superficially touched on the subject. Seven scientific articles are taken from these literature studies, a further 13 scientific articles are selected after a literature search. A total of 20 scientific articles that meet the inclusion criteria are included and analyzed. In order to assess whether Swedish authorities implement effective police and legislative measures with the aim of increasing traffic safety for pedestrians, a comparison is made between the measures proposed by the research and the measures that two Swedish authorities, the Swedish Transport Agency and the Swedish Police Agency, present in their documents Aktionsplan för säker vägtrafik 2022–2025 - 250 åtgärder som 33 myndigheter och aktörer avser att vidta för ökad trafiksäkerhet samt Polismyndighetens strategi inom trafiksäkerhetsarbetet. Results: The study shows that targeted police measures aimed at fining individuals who break traffic rules in a certain place during a certain time have a positive effect on pedestrian traffic safety. The focus should be on increasing rule compliance and reducing the number of speed limit violations by motorists, as well as encouraging pedestrians not to walk against red lights. The legislative measures that the scoping review shows have the best effect are lowering the speed limit and car-free zones on stretches of road where many pedestrians are moving. Conclusions: The conclusions that thos scoping review draws are that further research is required in the connection between reduced average speed and the number of traffic accidents which leads to personal injuries to pedestrians, as well as the part of police and legislative measures in different action programs that include several different measures that are implemented together. The police authority is working towards a reduced average speed of motorists through targeted police measures, which is in line with the measures proposed by the existing research in the area. However, there is nothing in the document about targeted police measures against pedestrians in order to increase rule compliance by that group, which is something that the Police Authority is recommended to develop. In the Swedish Transport Agency's action plan for the years 2022-2025, no measures are mentioned to increase pedestrian traffic safety linked to traffic accidents. Only measures to reduce the number of fall injuries are presented. The Swedish Transport Agency is therefore recommended to further develop its action plan and also have it include legislative measures such as reduced speed limits or car-free zones on road sections where many pedestrians move.

Digitalization has been reshaping the media landscape in recent years, often conveying an implicit promise of becoming less dependent on physical resources. At the same time, the current understanding of digital reading goes beyond dedicated e-readers or definable digital media products such as magazines or newspapers. In fact, it must be perceived as a function or service obtained from existing and ever-expanding “digital ecosystems”. There is furthermore a clear and unambiguous trend that relatively small and mobile devices are on the rise for consuming all kinds of media. Next to potentially enabling environmental gains compared to traditional paper-based media consumption, there are agreeing indications of a shift from overall electricity consumption dominated by end-user devices towards an increasing importance of less tangible data transmission networks and data centers. Therefore, a bottom-up analysis is deemed to compliment more general top-down observations and assessments. To this end, an elaborated reference scenario is proposed as to bridge the mere analytical method of Life Cycle Assessment (LCA) with behavioral aspects based on German market observations and surveys. The prevailing aim of this study is to detect environmental hot-spots and absolute impacts linked to the service of accessing text-based content via connected electronic devices. In doing so, this study takes the position that both types of media consumption – digital and paper-based - are incommensurable due to the very evident differences in provided functions, markets, and industries. Therefore, an attributional and stand-alone LCA is considered appropriate. The perceived current situation (reference scenario) evolves around substantiated estimates and assumptions concerning production of devices, use of devices as well as operation of essential data transmission network components. Looking at potential hot-spots, electricity consumption linked to data transmission could be a decisive factor for the environmental performance of digital reading. However, the actual importance of data transmission infrastructures depends on both methodological choices and a range of parameters or trends. For instance, the relative importance is shifted when more recent estimates of electricity intensities are incorporated. Depending on actual and localized electricity intensity of data transmission, the amount of data required to provide an expected function may inhibit environmental potentials of digital media consumption. Postulating average annual consumption of digital contents and assuming actual substitution of equivalent printed media products, about 50 kg CO2-equivalents. could potentially be avoided. This theoretical potential is based on the calculated global warming potential (GWP) associated with digital reading according to the reference scenario which amounts to about 29 kg CO2-equivalents. Therefore, this study supports findings from previous studies that indicated environmental benefits of digital reading. Compared to other functions or services (e.g. video/music streaming, podcasts, audio books) embedded in the same “digital ecosystems”, reading requires little amount of data. If allocation of upstream effects is based on time, the relative importance of data transmission networks could be gauged and compared by adopting a “data-to-service time” ratio. Taking the reference scenario as a starting point, a perceivable ratio for digital reading is 0.015 GB/h, including systemic inefficiencies. In contrast, streaming of high-definition video contents can easily consume 3 GB/h, a 200-fold increase. The audience of this study comprises providers of digital reading services and/or other media services as well as end-users as integral element in “digital ecosystems”. Besides, the report proposes a conceptual assessment framework which can be applied to other contemporary digital services or functions. / De senaste åren har digitalisering omformat medielandskapet, med ett implicit löfte om att minska beroendet av fysiska resurser. Dessutom finns det tydliga trender som pekar mot en ökad användning av små, mobila enheter för att konsumera alla sorters media. En uppdaterad bottom-up analys bedöms komplettera mer generella observationer och bedömningar. Om man antar årliga genomsnittliga konsumtionsmönster i Tyskland, så är tillverkningen av elektroniska slutanvändarenheter – oavsett om de är till för enskilda ändamål (e-läsare) eller om de är multifunktionella (smartphone, surfplatta) – onekligen en miljömässigt kritisk punkt för digitalt läsande. Elförbrukningen, som sker i samband med dataöverföringen, kan också vara en avgörande faktor för den övergripande miljöpåverkan av digital läsning. Dock beror den faktiska påverkan av dataöverförningsinfrastrukturer dels på metodologiska val men även på ett antal andra parametrar och trender. Genom att undersöka indikatorn för global uppvärmning kan denna studie konstatera att resultaten stödjer tidigare forskning, som redan pekar på de miljömässiga vinsterna av digitalt läsande. Målgruppen för denna studie innefattar både distributörer av digitala läs-tjänster och/eller andra media tjänster såväl som slutanvändare som ett integrerat element i ”digitala ekosystem”.

In Anbetracht der enormen Verbesserung der HCV-Therapie durch die Entwicklung hochwirksamer und direkt wirkender Virostatika (DAA) dient diese Studie der Analyse von HCV-Koinfektionen unter den gemeldeten HIV-Neudiagnosen in Deutschland. Zunächst wurde die Eignung zweier kommerzieller HCV Ag/Ab ELISAs, dem Murex (Abbott) und dem Monolisa (Bio-Rad), zum Nachweis von HCV in getrockneten Serumspots (DSS) verglichen. Der Murex-ELISA zeigte sich sensitiver bei Antigen-positiven Plasma-HCV-Serokonversionsproben während der Monolisa eine höhere Sensitivität bei HCV-Antikörper-positiven DSS-Eluaten. Des Weiteren wurde ein Aviditätstest zur Unterscheidung von neuen und bereits länger bestehenden Infektionen bei einem Aviditätsindex Cut-off von 40% und einem Zeitraum von 364 Tagen etabliert. Von den insgesamt 6.097 untersuchten Proben der Jahre 2015-2017 waren 396 HCV-ELISA-reaktiv (6,5%). Von diesen wurden 256 (64,6%) als aktive und 140 (35,4%) als ausgeheilte Infektionen identifiziert. Ein hoher Anteil der HCV-Koinfektionen wurde bei intra-venösen Drogenkonsumenten (77,8%, n=168/216), in der Altersgruppe der 30-39 Jährigen (9%, n=179/1.978) und bei Menschen osteuropäischer Herkunft (38,3%, n=124/324) beobachtet. Im Vergleich zum Jahr 2016 hat sich der Anteil der ausgeheilten Infektionen im Jahr 2017 bei der Gesamtzahl der Patienten (p<0,01) sowie insbesondere bei Personen ausländischer (p<0,01) und osteuropäischer (p<0,05) Herkunft erhöht. Die HCV Subtypen (St)-1a, St-3a und St-1b waren mit 33,9% (n=79/233), 33,5% (n=78/233) und 23,3% (n=52/233) vorherrschend. Der Anteil der St-1a- und St-1b-Proben, deren HCV-Sequenz gegen DAAs resistent ist, war in allen untersuchten Jahren hoch (25%-42,9%). Der beobachtete Anstieg des Anteils der ausgeheilten HCV-Koinfektionen kann auf die DAA-Therapie zurückgeführt werden. Es sind jedoch weitere Anstrengungen erforderlich, damit Gruppen mit weiterhin hoher HCV-Prävalenz von dieser Behandlung profitieren. / In light of the major shift in HCV therapy resulting from the availability of highly potent direct acting antivirals (DAA), this study performed a surveillance of HCV coinfections among reported HIV new diagnoses in Germany. First the suitability of two HCV Ag/Ab ELISAs, Murex (Abbott) and Monolisa (Bio-Rad) for dried serum spots (DSS) was compared. The Murex was more sensitive in antigen positive plasma HCV seroconversion samples while the Monolisa showed a higher sensitivity in HCV antibody positive DSS eluates. Furthermore, an avidity test was established to distinguish between new and already longer existing infections at an avidity index cut-off of 40% and a period of 364 days. Of the total of 6,097 samples examined for the years 2015-2017, 396 were HCV ELISA reactive (6.5%). Of these, 256 (64.6%) were identified as active and 140 (35.4%) as resolved infections. A high proportion of HCV coinfections were observed in intravenous drug users (77.8%, n=168/216), in the age group 30-39 years (9%, n=179/1,978) and in people of Eastern European origin (38.3%, n=124/324). Compared to 2016, the proportion of resolved infections in 2017 has increased in the total number of patients (p<0.01) and especially in people of foreign (p<0.01) and Eastern European (p<0.05) origin. HCV subtypes (St)-1a, St-3a, and St-1b were predominant with 33.9% (n=79/233), 33.5% (n=78/233) and 23.3% (n=52/233), respectively. The proportion of St-1a and St-1b samples whose HCV sequence has resistance to DAAs was high in all diagnoses years (25%-42.9%). The observed increase in the proportion of resolved HCV coinfections can be attributed to DAA therapy. However, further efforts are needed to ensure that groups with continued high HCV prevalence benefit from this treatment.

This PhD Thesis has been immersed in investigating the holistic experience of way-finding in buildings by people who have a range of visual ability. Previous research studies, spanning across a broad spectrum of disciplines, have focused on various characteristics of human way-finding (Arthur and Passini, 1992;Lynch, 1960;Downs and Stea, 1973). It is specifically recognised that the built environment is failing people with visual loss (Barker et al., 1995) and the strategic task and skill of way-finding within a building is a particular problem (Arthur and Passini, 1992). Under the social model of disability (Oliver, 1990) this is recognised as a form of architectural disablement (Goldsmith, 1997). There are few evidence-based studies of way-finding in a building. Furthermore, there are no studies of real-life experiences of way-finding undertaken by real-life participants who have a range of visual ability within the context of a real-life building. This leads to a research question: What are the design issues revealed by participants who have a range of visual ability as they way-find in a large public building? This doctoral research, based within the discipline of architecture, focuses on the holistic experiential components of a Journey (Myerson, 2001;Harper and Green, 2000). It coins and defines the term Way-finding Hot-spot as it explores the events [positive and negative] which are experienced and therefore impact on a Way-finding Journey around a building. To fill an important gap in the current knowledge a research enquiry, based on a user-centred design approach, was implemented. Exploratory in nature, the methodology was inductive and it evolved throughout the study. A series of Research Principles, borrowed from the established methodologies of Grounded Theory (Glaser, 1968) and Case Study (Yin, 2003a;Yin, 2003b), guided this study. Ten participants [with varying degrees of visual ability, different ages and other forms of disability] undertook a Way-finding Scenario designed to evaluate both existing memories of way-finding and present way-finding experience. This was composed of a Purposeful Conversation (Burgess, 1982) and a context specific Way-finding Task. The study has produced a large amount of data based on user experience in a real-world way-finding context – this has not been done before. Participant data contributed to a new Theory of Way-finding – The Experiential Charting of a Way-finding Journey – which derived from experiential data, was found to be composed of three elements: Journey Stages, Tasks Components and Communication Requirements. This thesis presents detailed findings which generate dialogue in the design of way-finding systems suitable for a diverse range of way-finders. It provides a research-based foundation to open the problem area and provide an insight into the issues people with different visual abilities encounter as they undertake a Way-finding Journey around a building. It generates a greater understanding of the problems and joys of way-finding in a building which will be of use in professional practice across disciplines of architecture and design as well as in areas of rehabilitation, policy-making and academia. This research is a start, but it is not the end. Future research questions have been revealed and these, combined with further reviews of literature and creative use of method, will further explore the phenomenon of way-finding within the context of buildings.

Made available in DSpace on 2014-07-29T16:23:34Z (GMT). No. of bitstreams: 1 Tese Ricardo Dobrovolski.pdf: 1981880 bytes, checksum: 8c60352c3d999171ab957f065b32a9db (MD5) Previous issue date: 2012-04-10 / Agriculture is the human activity with the greatest impact on the environment. Specifically, it represents the greatest threat to biodiversity. In the future, this activity should expand due to population growth, increased consumption and production of biofuels from food. To understand the possible impacts of this expansion on biodiversity, we used scenarios of land use change between 1970 and 2100 from IMAGE (Integrated Model to Access Global Environment) to test the following hypotheses: (i) areas considered as global priorities for conservation by international NGOs will be preferentially impacted by agricultural expansion in the XXI century, (ii) there is a conflict between the priority areas for carnivores conservation and agricultural expansion, and this conflict can be reduced by incorporating information on agricultural expansion in the prioritization process, (iii) the integration among countries for conservation planning may benefit both biodiversity and agricultural productivity, (iv) Brazilian protected areas will be impacted by agricultural expansion in the future and this impact will differ between protected areas of integral protection and those of sustainable use. We found that: (i) the impact on priority areas for conservation depends on the criteria by which they were set, so that areas defined by its high vulnerability are currently most affected than those of low vulnerability. Throughout the XXI century this impact is expected to increase, although the difference between the two types of priorities remains, except for High Biodiversity Wilderness Areas, defined by their low vulnerability in current time, but for which most pessimistic scenarios forecast an impact similar to priority areas of high vulnerability, (ii) there is a high spatial congruence between areas with high agricultural use in the future and priority areas for conservation of carnivores. This conflict can be reduced if the prioritization process include information on agricultural expansion; this incorporation, however, causes a profound change in the distribution of priority areas and reduces the number of protected carnivore populations, (iii) the integration of countries to create a set of priority areas for conservation that represents 17% of the land surface can protect 19% more mammal populations without reducing food production, compared to a strategy in which each country seeks to protect its territory independently, and (iv) the impact of agriculture in Brazil is expected to increase until the end of the century, threatening even the protected areas and their surroundings. This impact, however, should not be different between areas of sustainable use and those of integral protection. We conclude that agricultural expansion should remain a major threat to biodiversity in the future, even in areas of special interest for conservation. Conservation actions should be planned taking into account this threat in order to reduce their potential impacts. For this, countries like Brazil should strengthen its surveillance on agricultural expansion and on how this activity is developed. Furthermore, the integration of international conservation efforts should be pursued, given its benefits for biodiversity and food production. Finally, humanity must choose methods of agricultural production that reduce its impacts, including avoiding its future expansion, so as to meet the increasing needs of a human population globally. / A agricultura é a atividade humana com maior impacto sobre o ambiente. Particularmente, ela representa a maior ameaça à biodiversidade. No futuro, essa atividade deve expandir-se com o aumento populacional humano, o aumento do consumo e a produção de biocombustíveis a partir dos alimentos. Para entender os possíveis impactos dessa expansão sobre a biodiversidade, nós utilizamos cenários de mudança de uso do solo entre 2000 e 2100 do IMAGE (Integrated Model to Access Global Environment) para testar as seguintes hipóteses: (i) as áreas consideradas como prioridades globais de conservação pelas ONGs internacionais serão preferencialmente impactadas pela expansão agrícola no século XXI; (ii) há um conflito entre áreas prioritárias para a conservação de carnívoros e a expansão agrícola e esse conflito pode ser reduzido com a incorporação da informação sobre expansão agrícola no processo de priorização; (iii) a integração entre os países para o planejamento da conservação pode ser favorável à proteção da biodiversidade e à produção agrícola; (iv) no Brasil, as áreas protegidas serão impactadas pela expansão agrícola no futuro e esse impacto será diferente entre áreas de proteção integral e áreas de uso sustentável. Nós encontramos os seguintes resultados: (i) o impacto sobre as áreas prioritárias para a conservação depende dos critérios pelos quais elas foram definidas, assim, as áreas definidas por sua alta vulnerabilidade estão atualmente mais impactadas do que áreas de baixa vulnerabilidade. Ao longo do século XXI, o impacto geral da agricultura deve aumentar, mas a diferença entre os dois tipos de prioridades se mantém, exceto para as High Biodiversity Wilderness Areas, definidas por sua baixa vulnerabilidade, mas que nos cenários mais pessimistas podem ter um impacto agrícola semelhante ao das áreas de alta vulnerabilidade; (ii) há uma alta congruência espacial entre áreas com elevado uso agrícola no futuro e áreas prioritárias para a conservação de carnívoros; esse conflito pode ser reduzido se o processo de priorização incluir as informações sobre a expansão agrícola; a incorporação dessa informação, entretanto, provoca uma profunda alteração na distribuição das áreas prioritárias e reduz o número de populações de carnívoros protegidas; (iii) a integração entre os países para a criação de um conjunto de áreas prioritárias para conservação que represente 17% da superfície terrestre pode proteger 19% mais populações de mamíferos sem reduzir a produção de alimentos, se comparada a uma estratégia em que cada país busque proteger seu território independentemente; (iv) o impacto da agricultura no Brasil deve aumentar até o fim do século XXI, ameaçando inclusive as áreas protegidas e o seu entorno. Esse impacto, porém, não deve ser diferente entre as áreas de uso sustentável e aquelas de proteção integral. Assim, a expansão agrícola deve continuar a ser uma importante ameaça à biodiversidade no futuro, atingindo inclusive áreas de especial interesse para a conservação. As ações de conservação devem ser planejadas levando em consideração essa ameaça, a fim de reduzir seus impactos potenciais. Para isso, países como o Brasil devem reforçar sua vigilância sobre a expansão agrícola e a maneira como essa atividade é desenvolvida. Além disso, a integração internacional dos esforços de conservação deve ser buscada, dados seus benefícios para a biodiversidade e para a produção de alimentos. E por fim, a humanidade deve optar por formas de produção agrícola que reduzam seus impactos, inclusive evitando sua expansão futura, mas que possam satisfazer as necessidades da população humana globalmente.

This thesis focuses on low-cost microextraction techniques and their application for purification and preconcentration of biological samples, specifically on the experimental study of supported liquid membrane (SLM) extraction. The described microextraction technique uses commercially available filtration plates as the extraction units and allows the extraction of basic drugs from biological samples of urine and blood (in the form of dried blood spots). The experimental part includes the optimization of microextraction conditions of basic drugs from real samples through a SLM coupled in-line to lab-made capillary electrophoresis. The basic optimization of microextraction conditions involved selecting the appropriate organic phase for membrane impregnation (1:1 mixture of ENB and DHE), appropriate agitation speed for sample convection during extraction (1000 rpm), and optimal ratio of donor to acceptor volumes for high preconcentration of the analytes (400:15 µL). After basic optimization, the effect of donor alkalization with NaOH on extraction recovery (ER) was investigated. For all matrices used (saline solution, undiluted human urine samples, human capillary blood eluted from dry blood spots with deionized water), the highest ER values were achieved using a neutral donor and an acidic acceptor. The extraction time (60 minutes) was optimized based on the time profile of the microextraction for 120 minutes. This optimized microextraction method is suitable for the determination of basic drugs in real matrices with sufficient sample clean-up, preconcentration and ER values.

Only in English.

Purines are organic compounds with miscellaneous functions that are found in all living organisms in complex molecules such as nucleotides, nucleosides or as purine bases. The natural balance of purine levels is maintained by their synthesis, recycling and degradation. Excess purines are excreted in the urine as uric acid. Purine nucleotides may be recycled by salvage pathways catalysing the reaction of purine base with phosphoribosyl pyrophosphate. A completely new central molecule of purine metabolism, inosine monophosphate, can be synthesized from precursors during the de novo purine synthesis (DNPS). DNPS involves ten steps catalysed by six enzymes that form a multienzymatic complex, the purinosome, enabling substrate channelling through the pathway. DNPS is activated under conditions involving a high purine demand such as organism development. Currently, three DNPS-disrupting disorders have been described: ADSL deficiency, AICA-ribosiduria and PAICS deficiency. All three disorders are caused by genetic mutations leading to the impaired function of particular enzyme causing insufficient activity of respective DNPS step, manifested biochemically by accumulation of substrate of deficient enzyme, biologically by disruption of purinosome formation and clinically by unspecific neurological features,...