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The roles of RNA helicases and other ribosome biogenesis factors during small subunit maturationDavila Gallesio, Jimena 27 August 2019 (has links)
No description available.
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Liu Shifu (1884-1915) a Chinese anarchist and the radicalization of Chinese thought /Chan, Pik-chong Agnes Wong. January 1979 (has links)
Thesis--University of California at Berkeley. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 256-282).
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Liu Shifu (1884-1915) a Chinese anarchist and the radicalization of Chinese thought /Chan, Pik-chong Agnes Wong. January 1979 (has links)
Thesis--University of California at Berkeley. / Includes bibliographical references (leaves 256-282). Also issued in print.
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Desenvolvimento de novas abordagens moleculares baseadas em PCR (Reação em Cadeia da Polimerase) para detecção gênero-específica de plasmodiumMaria Lapa Montenegro, Lílian January 2002 (has links)
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Previous issue date: 2002 / Oligonucleotídeos foram construídos com base na sequência primária do
gene codificando o rRNA de Plasmodium para amplificar DNA de P. falciparum,
P. vivax, P. malariae e P. ovale, de maneira gênero-específica. Três sistemas
de PCR foram utilizados: PCR simples, hemi-nested PCR convencional e
hemi-nested PCR em um único tubo, desenvolvidos em nosso laboratório. Na
PCR simples, composta de 30 ciclos, foram utilizados os oligonucleotídeos GJ1
e HR842 (20 pmol/50μl), já testados por nosso grupo. Na hemi-nested PCR
convencional utilizou-se três oligonucleotídeos (GJ1, PGFO3 e HR842), em
duas reações sequenciais, sendo o PGFO3 construído durante o
desenvolvimento do presente trabalho, visando a detecção do gênero
Plasmodium. O par GJ1 e HR842 foram utilizados como oligonucleotídeos
externos na primeira reação, e o PGFO3 como interno, ancorado ao HR842 na
segunda reação. A hemi-nested PCR em um único tubo consistiu em 60
ciclos (92ºC, 30s; 58ºC, 30s e 72ºC, 45s), e concentrações limitantes de
oligonucleotídeos externos (4 pmols/50μl) participavam da PCR sem
competição com os oligonucleotídeos internos durante os primeiros 15 ciclos
da reação e 40 pmol/50μl de primers internos (imobilizados na face interna da
tampa do microtubo) foram introduzidos na PCR no 16º ciclo. As concentrações dos outros componentes da reação foram as mesmas utilizadas nas reações
convencionais de PCR. Observou-se que a quantidade mínima de DNA
genômico detectada pela PCR simples, hemi-nested PCR e hemi-nested
PCR em um único tubo foi de 10 pg; 0,01 pg e 0,1 pg, respectivamente. Apesar
da hemi-nested PCR em um único tubo ter sido menos sensível que a heminested
PCR convencional, é muito mais simples e conveniente, já que o risco
de contaminação cruzada é bem menor. Esses sistemas moleculares de
diagnóstico podem ser usados em situações quando se requer uma alta
sensibilidade e especificidade, tais como na avaliação da eficiência de
quimioterapia, detecção precoce de infecção e prevenção de transmissão a
partir de pacientes hipoparasitêmicos
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Evoluce skupiny Retortamonadida (Eukaryota: Excavata: Fornicata) / Evolution of Retortamonadida (Eukaryota: Excavata: Fornicata)Smejkalová, Pavla January 2010 (has links)
Retortamonads (Retortamonadida; genera Chilomastix and Retortamonas) are a small group of protists comprising intestinal commensals of both vertebrates and invertebrates and one free-living species of the genus Chilomastix. Molecular phylogenetic studies showed that retortamonads are closely related to diplomonads, Carpediemonas, Dysnectes, Hicanonectes, Kipferlia and two undescribed lineages of free-living Carpediemonas-like organisms, together forming the monophyletic excavate group Fornicata. For a long time Retortamonadida have been assumed to be a monophyletic group. However, first molecular phylogenetic study including sequence data from both Retortamonas and Chilomastix suggested that Retortamonadida are paraphyletic and that diplomonads branch within Retortamonadida. Retortamonads still remain poorly studied protist group and their phylogeny is unclear. We sequenced and analysed SSU rDNA of ten Retortamonas and four Chilomastix SSU rDNA sequences. In addition, we sequenced SSU rDNA of an undescribed enteromonad lineage. The phylogenetic tree of Fornicata was largely unresolved and the phylogenetic position of the genus Chilomastix remained unclear. On the other hand, the genus Retortamonas and diplomonads formed a robust clade. Retortamonas sequences split into three host-specific lineages. The...
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Liang Sicheng, 1901-1972 the master of modern Chinese architecture = Liang Sicheng (yi jiu ling yi zhi yi jiu qi er) : Zhongguo xian dai jian zhu de zhe jiang /Ng, Wing-fai, January 1991 (has links)
Thesis (M.A.)--University of Hong Kong, 1991. / Added title in Chinese. Also available in print.
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Phylogenetic relationships and population structure of coccidia in rodent families Muridae and ArvicolidaeMÁCOVÁ, Anna January 2013 (has links)
Population structure and phylogenetic relationships were studied in coccidia parasitizing the rodent families Muridae and Arvicolidae, in 40 localities in 14 European countries. Sequences of mitochondrial gene for cytochrome c oxidase subunit I (COI) and nuclear 18S rRNA gene (SSU) were used for phylogenetic analyses and for reconstruction of evolutionary relationships among coccidian species.
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Prevalence and molecular characterization of \kur{Cryptosporidium} spp. in dairy cattle in South Bohemia, the Czech Republic / Prevalence and molecular characterization of \kur{Cryptosporidium} spp. in dairy cattle in South Bohemia, the Czech RepublicONDRÁČKOVÁ, Zuzana January 2009 (has links)
The prevalence and molecular characterization of Cryptosporidium spp. in slaughtered cattle 6 months and older was performed. Three species of Cryptosporidium were identified. A subtype of C. parvum was obtained.
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Fylogeneze archaméb / Phylogeny of ArchamoebaePtáčková, Eliška January 2010 (has links)
Archamoebae is a small group of anaerobic protists belonging to the eukaryotic supergroup Amoebozoa. Historically, they were regarded as primitively amitochondriate. However, a mitochondrial remnant has been found in some archamoebae. Phylogenetic analyses showed that Archamoebae are closely related to the aerobic slime moulds (Mycetozoa). Trophozoites of archamoebae are amoeboflagellates or aflagellated amoebae. The group includes both parasitic (Entamoeba, Endolimax and, possibly, Endamoeba and Iodamoeba) and free-living (Mastigamoeba, Mastigella, Pelomyxa) genera. The genus Mastigina comprises both endozoic and free-living representatives. Flagellated genera Mastigina, Mastigamoeba, Mastigella and Pelomyxa possess a single basal body associated with a microtubular cone which may or may not be associated with nucleus. The cone is a common feature for Archamoebae and mycetozoan slime moulds. The phylogeny of Archamoebae has not been fully elucidated yet and the taxonomy of free-living representatives is confusing. In the present study, we obtained 42 stable isolates of free-living Archamoebae. We sequenced and analyzed SSU rDNA of 15 of them. The Archamoebae split into five lineages. Based on TEM, we were able to recognize genera Mastigamoeba and Mastigella. The isolate IND8 probably represents a new...
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Health status of children left behind in Sichuan rural areas :a cross-sectional studyChoi, Weng I January 2018 (has links)
University of Macau / Institute of Chinese Medical Sciences
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