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Background: Understanding lactate levels can provide important information aboutour body’s condition. For athletes, this can improve their training and prevent earlyfatigue. In healthcare, monitoring lactate can provide valuable information and potentially prevent life-threatening episodes. Lactate can be measured non-invasively byanalyzing sweat. This is advantageous over the typical blood sampling since it is saferand pain-free. Sweat can be stimulated by using a method called iontophoresis. Itapplies a small current between two electrodes placed on the skin’s surface, deliveringsubstances to the inner layer of the skin. Objectives: The aim of this study was to design a device that implements iontophoresis to activate sweat production and uses a microfluidic system to collect thesweat and deliver it to a lactate sensor and provide a signal. Methodology: A device was designed in AutoCAD and 3D printed. It was improvedby trial and error. A sweat collecting test was performed to validate the iontophoresissystem. The efficiency of the microfluidic system was tested by recording the time ittakes to collect enough sweat to get a lactate signal. Finally, calibration tests wereperformed to validate the lactate signal in the form of batch-mode and flow-mode. Results: The sweat collection test produced 27 µL of sweat in 15 minutes and 49 µLin 30 minutes. The microfluidic system delivered sweat to the sensor and activated itin less than 3 minutes. The linearity of the batch-mode calibration, R2-value, was0.9994, and for the flow-mode it was 0.8908.Conclusions: The iontophoresis system stimulated sweat production, which themicrofluidic system delivered to the lactate sensor successfully. The lactate sensorwas implemented into the device, and a signal was detected. However, it could not becalibrated efficiently enough to display the electric signal as a lactate concentration.

The purpose of this thesis is to showcase the importance of God-inspired Theatre and to manifest the transformative effects of living in accordance to the Word of God. In order to share my vision for theatre such as this, I will examine the biblical elements in Zora Neale Hurston's short story Sweat (1926). I will write a stage adaptation of the story, while placing emphasis on the biblical lessons that can be used for God-inspired Theatre. When viewing the stage adaptation based on Sweat, the audience members will understand how God-inspired Theatre aims to help members of society utilize their gifts and abilities to assist others in achieving spiritual stability. The members of the audience will also be informed of my vision to use this piece to inspire others to embrace cultural awareness and sensitivity. This is my vision--helping others to walk in their God-ordained destiny. With this in mind, I am using Sweat as a proposed play because it is closely related to the creation account as recorded in the Old Testament Book of Genesis. In this play, Adam and Eve are replaced with the characters Sykes and Delia Jones. The creation account is a very influential testimony because it is known throughout humanity. Its popularity is due to the fact that the Old Testament is the commencement of the Christian Bible. For those of the Jewish faith, the collection encompasses the Torah, the first five books of the bible--the law for everyday living-- as well as the history of God's promise to them. For Christians, the Old Testament is just as sacred, but they view its religious meaning as incomplete without the life, teachings, death, and resurrection of Jesus Christ detailed in the New Testament. Also, Muslims trace their religious roots to some of the figures in the Old Testament although they deny the religious significance of the work as a whole. In essence, the Old Testament is crucial to Western Civilization. This is why Sweat is so powerful. It takes an extremely familiar testimony and shares pertinent messages that help people to become productive members of society. In order to show how effective Sweat is in helping others to live spirit-filled lives, I will use creative staging that will place the characters in the personal space of the audience members. I will achieve this by: having the actors enter and exit from the audience; allowing certain scenes to take place within the audience; and having the actors deliver some lines to various audience members. I feel that by making the audience a part of the production, it will cause them to see that they are not any different from the characters in the play. At some point in their lives, theatergoers have encountered--or been intimate with--an Adam, an Eve, a Sykes or a Delia. This will cause them to not see Sweat as just a play, but as a valuable life lesson, triggering self-examination and initiating renovated thinking that helps people to become culturally aware and spiritually sound. It is imperative that the biblical messages in Sweat are conspicuous. Whereas the narrator normally describes Delia's facial expression or feelings, I plan to write in scenes where her thoughts are audible. Some of her thoughts will include moments when she is praising and worshipping God. This is apparent because she starts to emerge as a woman of strength as the story progresses. Her relationship with God is cultivated on a daily basis. This is why she is able to tolerate her husband's foolishness. Her husband, Sykes, does not commune with God. To demonstrate his lack of communion with God, I will stage him being resistant to her times of worship--as he normally is according to the narrator. Clearly, the marriage is unbalanced. One partner is trying to please God, and the other is trying to please self. This is not how God intended marriage to be. In the New Testament Book First Peter, it states in the third chapter and seventh verse "husbands are to dwell with them in understanding, giving honor to the wife... being heirs together of the grace of life." Showing the burdensome consequences of destructing God's original design will pull on the hearts of audience members because they have encountered or known someone who is presently dealing with the consequences of this disobedient act. I will further reiterate the need for living a spirit-filled life by using costumes, scenic devices, and lighting to convey the godly and ungodly character traits that are embodied within the story. Through the use of colors and patterns, I will project the internal state of the character as in relation to God's instructions. I will work with a lighting designer in order to help convey the moods of the various scenes. The lighting techniques we choose will help to establish the thoughts and personalities of the characters. These feelings will transcend the minds of the audience and cause them to take the biblical messages into very deep consideration. The actors are the final ingredients in making Sweat an awe-inspiring, informative piece. Words are what they are, what one perceives them to be, while on paper. It is the job of the actor to give life to these words, cause them to live in the atmosphere, and to make the character come alive. Until the actor embodies the very heart of a character, the message in God-inspired Theatre will not be able to come forth and propel audience members to have a spiritual awakening. This is why people cannot just read Sweat. They must see the trials and journeys in order to receive life-changing revelations from the testimonies within the play.

No description available.

This study was conducted to explore the feasibility of using sleeves, along with both physiological and perceptual measurements, to assess the thermal comfort and heat strain in chemical protective clothing wear tests. The effect of body sites, i.e. the arms and the chest, and the effect of a Tyvek® coverall on skin temperature and sweat amount were investigated as well. Ten male subjects were selected from college students. Each subject was assigned to wear a garment of either T-shirt and pants or a changeable left-sleeved Tyvek® coverall with or without an experimental sleeve on the left arm. Three experimental sleeves were of the same style, but made of three different fabrics: Tyvek®, Pro/Shield I®, and Pro/Shield II®. Each subject wore an assigned garment and pedaled on a cycle ergometer in an environmentally-controlled room. Skin temperature, sweat amount, and subjective comfort evaluations were recorded and later analyzed by repeated-measures ANOVA. Results showed there is a feasibility in using a sleeve with a T-shirt/pants standard garment for assessing thermal comfort in chemical protective garment wear tests. The T-shirt/pants standard garment yielded more consistent and reliable results than did the Tyvek®-coverall standard garment. Under the same conditions, there were no significant differences in skin temperature and sweat amount among the left and right upper arms and the chest. The Tyvek® sleeve in this study impeded the transfer of heat by the greatest amount, followed by the Pro/Shield II® sleeve and the Pro/Shield I® sleeve, respectively. / Master of Science

Neste trabalho desenvolvemos um procedimento simples para a separação de nanoesferas ocas de carbono (do inglês Carbon Spherical Shells ou CSS) em diâmetros entre 400 e 500 nm utilizando centrifugação corroborado pelas análises realizadas na microscopia eletrônica de varredura e de transmissão. A análise de sua composição química, realizada através da técnica de fotoelétrons excitados por raios X, indicou que as CSS são constituídas de 79% de carbono e 21% de oxigênio em sua superfície, apresentando grupos funcionais carbonila e hidroxila. Plataformas sensoriais distintas foram obtidas formando filmes homogêneos das CSS sobre o eletrodo de carbonno vítreo GCE (do inglês glassy carbon electrode ou GCE). Como resultado dos experimentos eletroanalíticos, observou-se o aumento da sensitividade do eletrodo GCE/CSS de acordo com a diminuição do diâmetro (500 até 400 nm) das CSS. As plataformas sensoriais GCE/CSS com 400 nm de diâmetro apresentaram maior sensitividade (0.02 μA µmol L-1) com um limite detecção de 0.2 μmol L-1. Os eletrodos GCE/CSS foram estáveis, apresentando pequena interferência de espécies concomitantes presentes na amostra e seu desempenho na quantificação de paracetamol em suor mostrou-se estatisticamente equivalente ao método padrão baseado em cromatografia líquida. / We applied a simple strategy, based upon centrifugation, to separate carbon spherical shells (CSS), in sizes varying from 400 to 500 nm, which is shown by the micrographs obtained in the Scanning and Transmission Electron microscopy analysis. In their surface, carbonyl and hydroxyl groups were present, constituting a composition of 21% of oxygen and 79% of carbon. The CSS were casted on a glassy carbon electrode\'s (GCE) surface, forming a thin film, and the resulting platform was used as a sensor. A trend was observed in the results obtained by the electroanalytical experiments: as the size of the CSS were reduced, the sensibility of the GCE/CSS platform towards paracetamol detection increased. The best attained result, namely the platform with the GCE and the 400 nm diameter CSS, have shown promising results, achieving sensitivity\'s value of 0.02 μA μmol-1 L. The proposed sensors were stable, displaying little interference from another species coexisting in the samples, and its performance towards paracetamol detection were statistically identical to the standard method for paracetamol detection based upon liquid chromatography.

Neste trabalho desenvolvemos um procedimento simples para a separação de nanoesferas ocas de carbono (do inglês Carbon Spherical Shells ou CSS) em diâmetros entre 400 e 500 nm utilizando centrifugação corroborado pelas análises realizadas na microscopia eletrônica de varredura e de transmissão. A análise de sua composição química, realizada através da técnica de fotoelétrons excitados por raios X, indicou que as CSS são constituídas de 79% de carbono e 21% de oxigênio em sua superfície, apresentando grupos funcionais carbonila e hidroxila. Plataformas sensoriais distintas foram obtidas formando filmes homogêneos das CSS sobre o eletrodo de carbonno vítreo GCE (do inglês glassy carbon electrode ou GCE). Como resultado dos experimentos eletroanalíticos, observou-se o aumento da sensitividade do eletrodo GCE/CSS de acordo com a diminuição do diâmetro (500 até 400 nm) das CSS. As plataformas sensoriais GCE/CSS com 400 nm de diâmetro apresentaram maior sensitividade (0.02 μA µmol L-1) com um limite detecção de 0.2 μmol L-1. Os eletrodos GCE/CSS foram estáveis, apresentando pequena interferência de espécies concomitantes presentes na amostra e seu desempenho na quantificação de paracetamol em suor mostrou-se estatisticamente equivalente ao método padrão baseado em cromatografia líquida. / We applied a simple strategy, based upon centrifugation, to separate carbon spherical shells (CSS), in sizes varying from 400 to 500 nm, which is shown by the micrographs obtained in the Scanning and Transmission Electron microscopy analysis. In their surface, carbonyl and hydroxyl groups were present, constituting a composition of 21% of oxygen and 79% of carbon. The CSS were casted on a glassy carbon electrode\'s (GCE) surface, forming a thin film, and the resulting platform was used as a sensor. A trend was observed in the results obtained by the electroanalytical experiments: as the size of the CSS were reduced, the sensibility of the GCE/CSS platform towards paracetamol detection increased. The best attained result, namely the platform with the GCE and the 400 nm diameter CSS, have shown promising results, achieving sensitivity\'s value of 0.02 μA μmol-1 L. The proposed sensors were stable, displaying little interference from another species coexisting in the samples, and its performance towards paracetamol detection were statistically identical to the standard method for paracetamol detection based upon liquid chromatography.

Submitted by Rebeka Godeiro (rebeka_carvalho@hotmail.com) on 2018-05-21T11:54:00Z No. of bitstreams: 1 NÁGELA MARIA HENRIQUE MASCARENHAS - DISSERTAÇÃO ZOOTECNIA 2018.pdf: 1661338 bytes, checksum: bfa1f2a8ffad9f48af7f40dedf2de4e9 (MD5) / Made available in DSpace on 2018-05-21T11:54:00Z (GMT). No. of bitstreams: 1 NÁGELA MARIA HENRIQUE MASCARENHAS - DISSERTAÇÃO ZOOTECNIA 2018.pdf: 1661338 bytes, checksum: bfa1f2a8ffad9f48af7f40dedf2de4e9 (MD5) Previous issue date: 2018-02-23 / Objetivou-se avaliar o efeito de duas épocas do ano, sobre a adaptabilidade de caprinos e ovinos criados em sistema extensivo no semiárido paraibano, através de parâmetros fisiológicos, testes de adaptabilidade, características estruturais do pelame, taxa de sudação e estruturas do tegumento. Foram realizados dois experimentos consecutivos. No primeiro experimento utilizou-se 24 animais, sendo 12 ovinos Santa Inês e 12 caprinos Moxotó. Avaliados oito vezes durante os meses de julho a outubro de 2016. Foram mensurados: temperatura retal (TR), frequência respiratória (FR) e temperatura superficial (TS), o índice de tolerância ao calor (ITC) e o coeficiente de tolerância ao calor (CTC). O ITC revelou que os animais são adaptados as condições climáticas. O CTC das fêmeas mostrou-se mais elevados (P<0,05) que dos machos. De maneira geral, não houve diferença significativa entre as espécies de caprinos e ovinos para a temperatura retal e a frequência respiratória. A análise de variância revelou interação significativa (P<0,05) da espécie e dentro das épocas, sobre a TS. No segundo experimento foi utilizado um delineamento inteiramente casualizado, num esquema fatorial de 2x2 (2 espécies, 2 sexos) 2x2 (2 espécies e 2 sexos) com 5 repetições. Utilizou-se 20 animais, 10 ovinos Santa Inês e 10 caprinos Moxotó. Os animais foram mantidos em sistema extensivo, avaliados apenas uma vez durante o mês de novembro, da época quente do ano de 2016. As características avaliadas foram: epessura da capa de pelo (ECP), número (NP), comprimento (CP) e peso (PS) dos pelos, a densidade númerica (DN) e volumétrica (DV). A capacidade da taxa de sudação (TS) dos animais, além de estruturas do tegumento como: folículo piloso, glândulas sudoríparas (GSu) e espessura da epiderme (EEp). O sexo dos animais não influenciou (P>0,05) em nenhuma das variavéis avaliadas. com relação as caracteristicas do pelame, o CP dos caprinos foi mais elevado (P<0,05) que os ovinos, porém o PS e a DV dos ovinos foram mais elevadas (P<0,05). Os ovinos apresentaram número de GSu e uma EEp mais elevados (P<0,05) que os caprinos. A TS não apresntou diferenças estatisticas (P>0,05). Ambas espécies estudadas demonstram estar fisiologicamente bem adaptadas e com características estrurais do pelame e esturutras do tegumento favoráveis às condições climáticas do Semiárido. / The objective of this study was to evaluate the effect of two seasons of the year on the adaptability of goats and sheep raised in an extensive system in the Paraíba semi-arid region, through physiological parameters, adaptability tests, structural characteristics of the pelage, sweating rate and integument structures. Two consecutive experiments were performed. In the first experiment 24 animals were used, 12 Santa Inês sheep and 12 Moxotó goats. Evaluated eight times during the months of July to October 2016. Rectal temperature (TR), respiratory rate (FR) and surface temperature (TS), the heat tolerance index (ITC) and the heat tolerance coefficient (CTC). The ITC has revealed that the animals are adapted to the climatic conditions. Females CTC showed higher (P <0.05) than males. In general, there was no significant difference between goat and sheep species for rectal temperature and respiratory rate. The analysis of variance revealed significant interaction (P <0.05) of the species and within the seasons, on TS. In the second experiment, a completely randomized design was used in a factorial scheme of 2x2 (2 species, 2 sexes) 2x2 (2 species and 2 sexes) with 5 replicates. Twenty animals, 10 Santa Inês sheep and 10 Moxotó goats were used. The animals were kept in an extensive system, evaluated only once during the month of November, during the hot season of 2016. The characteristics evaluated were: cape layer thickness (ECP), number (NP), length (CP) and weight (PS) of the hairs, the numerical (DN) and volumetric (DV) density. The capacity of the sweating rate (TS) of the animals, as well as structures of the integument such as: hair follicle, sweat glands (GSu) and epidermis thickness (EEp). The sex of the animals did not influence (P> 0.05) in any of the evaluated variables. (P <0.05) than sheep, but PS and BV of sheep were higher (P <0.05). The sheep had a higher GSu number and a higher EPS (P <0.05) than the goats. TS had no statistical differences (P> 0.05). Both species have been shown to be physiologically well adapted and with the characteristics of the fur and sturgeons of the tegument favorable to the climatic conditions of the Semi - arid.