Nach einer Betrachtung der bestehenden Ansätze zur automatischen Graphgenerierung wurde eine Möglichkeit entwickelt, auf der Grundlage von OpenStreetMap-Innenraumkarten mit Hilfe einer bereits existierenden Implementierung des Straight Skeleton in Python automatisch Wege zu berechnen und diese wieder ins OpenStreetMap-Format zu überführen.:1. Einleitung 2. Überblick zur aktuellen Forschung 3. Anwendung des Straight Skeleton auf OpenStreetMap-Daten 3.1. Polyskel 3.2. Einlesen der Daten und Extrahieren der Türen und Räume 3.3. Kombination von Türen und Räumen 3.4. Generierung von Wegen mit Hilfe von polyskel 3.5. Kurze Wege zu längeren zusammenfassen 3.6. Wege vereinfachen 3.7. Wege im OpenStreetMap-Format speichern 4. Fazit und Ausblick Literaturverzeichnis Anhang

Call number: LD2668 .T4 1985 G73 / Master of Science

Thesis (MSc)--Stellenbosch University, 2005. / ENGLISH ABSTRACT: Introduction: Exercise increases skeletal muscle glucose uptake via AMP-activated protein kinase (AMPK) activation and GLUT4 translocation from cytosol to cell membrane. It also promotes glucose utilisation in type 2 diabetic patients via increased insulin sensitivity. Insulin stimulates GLUT4 translocation by activating P13- kinase and protein kinase B (PKB/Akt). We therefore postulated that a connection exists between these two pathways upstream of GLUT4 translocation. Understanding this connection is important in the development of treatment strategies for type 2 diabetes. This exercise-induced increase in AMP-activated protein kinase (AMPK) activation can be mimicked by a pharmacological agent, 5'-aminoimidazole-4- carboxamide ribonucleoside (AlGAR), which is converted intracellularly into 5'- aminoimidazole-4-carboxamide-ribonucleosidephosphate (ZMP), an AMP analogue. Aim: To investigate the effect of two pharmacological AMPK-activating compounds, ZMP and AlGAR, on the phosphorylation of AMPK, the phosphorylation of PKB/Akt as well as possible feedback on insulin-stimulated glucose uptake and GLUT4 translocation. Materials and Methods: Adult ventricular cardiomyocytes were isolated from male Wistar rats by collagenase perfusion and treated with 1 mM AlGAR or 1 mM ZMP in the presence or absence of 100 nM insulin or 100 nM wortmannin, an inhibitor of P13- kinase. Glucose uptake was measured via eH]-2-deoxyglucose (2DG) accumulation. PKB/Akt and AMPK phosphorylation and GLUT4 translocation was detected by Western blotting. Purinergic receptors were blocked with 8-cyclopentyl-1,3- dipropylxanthine (8CPT) and the effect on AMPK phosphorylation noted. Certain results were confinned or refuted by repeating experiments using the isolated rat heart model. Results: AICAR and ZMP promoted AMPK phosphorylation. Neither drug increased glucose uptake but in fact inhibited basal glucose uptake, although GLUT4 translocation from cytosol to membrane occurred. Both compounds also attenuated insulin stimulated glucose uptake. Wortmann in abolished glucose uptake and PKB/Akt phosphorylation elicited by insulin while, in the presence of wortmannin, AICAR and ZMP increased levels of PKB/Akt phosphorylation. Although AICAR and ZMP increased glucose uptake in skeletal muscle, this was not seen in cardiomyocytes. However both compounds increased GLUT4 translocation, clearly demonstrating that translocation and activation of GLUT4 are separate processes. 8CPT had no effect on the phosphorylation of AMPK by either AICAR or ZMP indicating that there was no involvement of the purinergic receptors. Conclusion: Although AICAR and ZMP increase glucose uptake in skeletal muscle, this was not seen in cardiomyocytes. Conversely, both compounds inhibited both basal and insulin stimulated glucose uptake despite increasing GLUT4 translocation. Inhibition of PI3-kinase in presence or absence of insulin unmasked hitherto unknown effects of AICAR and ZMP on PKB phosphorylation. / AFRIKAANSE OPSOMMING: Agtergrond: Oefening verhoog skeletspier glukose opname via AMP-geaktiveerde protein kinase (AMPK) aktivering en GLUT4 translokering vanaf die sitosol na die selmembraan. Dit verbeter ook glukose verbruik in tipe 2 diabetes pasiënte via verhoogde insulien sensitiwiteit. Insulien stimuleer GLUT4 translokering deur P13- kinase en protein kinase B (PKB/Akt) te aktiveer. Dit word dus gepostuleer dat daar 'n verbinding tussen hierdie twee paaie, wat beide betrokke is by GLUT4 translokering, bestaan. Dit is belangrik om hierdie verbinding te verstaan aangesien dit in behandelingstrategieë van tipe 2 diabetes geteiken kan word. Die oefening geïnduseerde verhoging in AMPK aktivering, kan deur 'n farmakologiese middel 5'- aminoimidasool-4-karboksamied ribonukleosied (AICAR), wat intrasellulêr omgesit word na 5'-aminoimidasool-4-karboksamied-ribonukleosiedfosfaat (ZMP), 'n AMP analoog, nageboots word. Doel: Om die effek van twee farmakologiese AMPK-aktiveringsmiddels, AICAR en ZMP, op die fosforilering van AMPK en PKB/Akt, sowel as moontlike effekte daarvan op insulien-gestimuleerde glukose opname en GLUT4 translokering, te ondersoek. Materiale en Metodes: Volwasse ventrikulêre kardiomiosiete is uit manlike Wistar rotharte geïsoleer d.m.v kollagenase perfusies en behandel met 1 mM AICAR of 1 mM ZMP in die teenwoordigheid of afwesigheid van 100 nM insulien of 100 nM wortmannin. Glukose opname is gemeet via intrasellulêre [3H]-2-deoksiglukose akkumulasie; PKB/Akt en AMPK fosforilering sowel as GLUT4 translokering is bepaal deur Western blot analises. Purinergiese reseptore is geblokkeer met 8-siklopentiel- 1,3-dipropielxanthien (8CPT) en die effek daarvan op AMPK fosforilering genoteer. Ten einde resultate wat in die geïsoleerde kardiomiosiet-model verkry is, te bevestig, is sekere eksperimente in die geïsoleerde rothart herhaal. Resultate: Beide AIGAR en ZMP stimuleer AMPK fosforilering. Die middels kan nie glukose opname verhoog nie, inteendeel, basale glukose opname is onderdruk alhoewel GLUT4 translokering vanaf die sitosol na die selmembraan wel plaasgevind het. Wortmannin kon insulien gemedieerde glukose opname en PKB/Akt fosforilering onderdruk. In die teenwoordigheid van wortmannin het beide AIGAR en ZMP PKB/Akt fosforilering verhoog. Alhoewel beide AIGAR en ZMP glukose opname in skeletspier verhoog, was dit nie die geval in kardiomiosiete nie. Beide middels het wel GLUT 4 translokering verhoog, wat duidelik demonstreer dat die translokering en aktivering van GLUT4, verskillende prosesse is. 8GPT het geen effek gehad op die fosforilering van AMPK deur AIGAR of ZMP nie, wat bewys dat daar geen betrokkenheid van die purinergiese reseptore was nie. Gevolgtrekking: Alhoewel AIGAR en ZMP glukose opname in skeletspier verhoog is dit nie die geval in kardiomiosiete nie. Beide middels inhibeer basale en insuliengestimuleerde glukose opname maar stimuleer GLUT4 translokeering. Inhibisie van PI3-kinase in die teenwoordigheid of afwesigheid van insulien, ontmasker voorheen onbekende effekte van AIGAR en ZMP op PKB/Akt fosforilering.

No description available.

The plant leaf is one of the most challenging natural objects to be realistically depicted by computer graphics due to its complex morphological and optical characteristics. Although many studies have been done on plant modeling, previous research on leaf modeling required for close-up realistic plant images is very rare. In this thesis, a novel method for modeling of the leaf shape based on the leaf venation is presented. As the first step of the method, the leaf domain is defined by the enclosure of the leaf boundary. Second, the leaf venation is interactively modeled as a hierarchical skeleton based on the actual leaf image. Third, the leaf domain is triangulated with the skeleton as constraints. The skeleton is articulated with nodes on the skeleton. Fourth, the skeleton is interactively transformed to a specific shape. A user can manipulate the skeleton using two methods which are complementary to each other: one controls individual joints on the skeleton while the other controls the skeleton through an intermediate spline curve. Finally, the leaf blade shape is deformed to conform to the skeleton by interpolation. An interactive modeler was developed to help a user to model a leaf shape interactively and several leaves were modeled by the interactive modeler. The ray-traced rendering images demonstrate that the proposed method is effective in the leaf shape modeling.

Master of Science / Department of Computing and Information Sciences / William H. Hsu / Automatic rigging is a targeting approach that takes a 3-D character mesh and an adapted skeleton and automatically embeds it into the mesh. Automating the embedding step provides a savings over traditional character rigging approaches, which require manual guidance, at the cost of occasional errors in recognizing parts of the mesh and aligning bones of the skeleton with it. In this thesis, I examine the problem of reducing such errors in an auto-rigging system and apply a density-based clustering algorithm to correct errors in a particular system, Pinocchio (Baran & Popovic, 2007). I show how the density-based clustering algorithm DBSCAN (Ester et al., 1996) is able to filter out some impossible vertices to correct errors at character extremities (hair, hands, and feet) and those resulting from clothing that hides extremities such as legs.

Sidladdningar på webbplatser har visat sig ha stor påverkan på hur användare beter sig på webbplatser. Redan efter 1 sekunds fördröjning börjar användarens beteende ändras. Laddningsindikatorer kan användas för att minska den upplevda hastigheten av sidladdningar. Det finns olika varianter av laddningsindikatorer som fungerar på olika sätt. En traditionell laddningsindikator är spinner och en nyare, mindre undersökt, heter skeleton screen.  Syftet med denna kandidatuppsats har varit att undersöka effektiviteten hos skeleton screen att påverka den upplevda hastigheten av sidladdningar. Dessutom var syftet att utforma och demonstrera ett test för att förse webbutvecklare med vägledning om hur liknande test kan genomföras i syfte att avgöra vilken laddningsindikator de bör välja att använda vid utveckling. En prototypisk webbsida skapades i två olika versioner, en med 2 sekunders fördröjning och en med 4 sekunders fördröjning vid sidladdning. För att ladda sidorna användes spinner eller skeleton screen. För att samla in användares upplevda hastighet av webbplatsen utfördes användartester där deltagarna för varje sida fick uppskatta hur lång tid nedladdningen tog. Totalt deltog 14 testpersoner i åldrarna 13–47 år. Resultatet visade på att skeleton screen påverkade testpersonernas upplevda hastighet som kortast i både versionerna. Medelvärdet av alla resultatet från testpersonerna visade på att vid 2 sekunders fördröjning upplevdes sidladdningar som 0.89 sekunder med skeleton screen och 1,95 sekunder med spinner. Vid 4 sekunders fördröjning upplevdes skeleton screen som 1,38 sekunder och spinner 3,81 sekunder. Trots att skeleton screen var den laddningsindikator som minskade upplevd hastighet mest, fanns det delade åsikter om vilken laddningsindikator testpersonerna föredrog baserat på design och tidigare erfarenheter. Skeleton screen verkar inte vara meningsfulla på sidor med enstaka produkter, utan effektiviteten av skeleton screen visades främst vid laddning av flera produkter.

A relação entre músicos e dores musculoesqueléticas é cada vez mais comum em centro de estudos musicais, tais como, universidades e orquestras. O objetivo desta dissertação foi analisar a razão de chance de ocorrência de dor, variáveis posturais e disfunção em violinistas na cidade de São Paulo. Foram avaliados 38 violinistas com idade de 21.5 ± 4.7 anos. A análise postural foi realizada por meio de fotogrametria com auxílio do software SAPO v.0.63 e de marcadores previamente colocados em referências ósseas, observadas nos planos frontais (anterior e posterior) e sagitais. A avaliação da dor foi realizada por meio da Escala Visual Analógica de Dor (EVA), questionário McGill e a disfunção musculoesquelética pelo questionário DASH. Para o cálculo da probabilidade de ter dor durante o estudo com o violino foi realizado um modelo de regressão logística onde todas as variáveis (posturais, idade, horas de prática e tempo de prática-anos) foram consideradas. Os resultados obtidos para o índice EVA e DASH foram analisados utilizando-se a técnica multivariada k-médias, sendo que as variáveis importantes para discriminação dos grupos em relação à EVA foram a postura da cabeça, a postura do ombro e a cifose torácica e para o índice DASH o desvio lateral da coluna e a inclinação da cabeça. Este estudo encontrou uma relação entre dor, disfunção e variáveis posturais nos violinistas na cidade de São Paulo. O índice DASH apresentou sinais de disfunção que se relacionaram com as variáveis posturais assim como o índice EVA. O Questionário McGill apresentou significância estatística na categoria mista e os violinistas com menos tempo de prática possuem uma média maior de dor / The relation between musicians and musculoskeletal pain is more and more common in musical research centers, like universities and orchestras. The aim of this thesis was to analyze the odds ratio of occurrence of pain, postural changes, and disabilities of violinists in São Paulo. It has been assessed thirty- eight violinists with a mean age of 21.5 ± 4.7 years. Postural analysis was carried out by means of Photogrammetry with the aid of the software SAPO v.0.63 and previously placed in bone markers, observed in the references front (anterior and posterior) and sagittal plane. The assessment of pain was accomplished using Visual Analog Pain Scale (VAS), McGill questionnaire and also Dash questionnaire for musculoskeletal dysfunction. For the calculation of the probability of occurrence of pain, a logistic regression model was employed and all variables related to posture, age, gender, hours of practice, and time of practice were considered. The results regarding the VAS and DASH were analyzed using multivariate techniques, k-media. The most important variables for the discrimination between the groups regarding the VAS scores were head and shoulder posture and thoracic kyphosis and for the DASH index, were the lateral spinal deviation and the head tilt. This study found a relationship between pain, dysfunction and postural variables in violinists in the city of São Paulo. The index DASH presented signs of dysfunction that related with postural variables as well as the VAS index. The McGill Questionnaire showed statistical significance in the mixed category and the violinists with less practice time have a higher average pain

A conquista do ambiente terrestre por caranguejos Brachyura é dependente da obtenção e reserva de água na câmara branquial durante o período de emersão. Uma maneira de se obter água do meio é através de tufos de cerdas hidrofílicas, capazes de captar água do solo por capilaridade. Sabe-se que essas cerdas hidrofílicas estão associadas à abertura de Müller e que a água captada é admitida na câmara branquial graças à diferença de pressão entre a câmara branquial e o meio externo, produzida pelos batimentos do escafognatito. Contudo, as modificações morfológicas esqueletais e apendiculares para a formação da abertura de Müller e a composição das cerdas do tufo não são conhecidos. Com o intuito de elucidar tais lacunas, foram estudados o esqueleto axial, apendicular e a quetotaxia de 22 espécies de caranguejos semiterrestres pertencentes às superfamílias Grapsoidea (Grapsidae, Sesarmidae, Varunidae e Gecarcinidae) e Ocypoidea (Ocypodidae e Ucididae). O estudo do esqueleto axial e apendicular revelou que a abertura de Müller é, na verdade, um canal complexo que pode ser composto por três componentes: o canal pleural, a calha apendicular e o tufo de cerdas. A água presente no substrato é captada pelo tufo de cerdas, é conduzida pela calha apendicular, passa pelo canal pleural para, enfim, ser admitida na câmara branquial. O tufo de cerdas é formado entre a face posterior do coxopodito do pereópode 3 e a face anterior do coxopodito do pereópode 4. Em Sesarmidae, o tufo também ocorre entre os coxopoditos dos pereópodes 2 e 3. Os representantes das superfamílias Grapsoidea e Ocypodoidea diferem quanto à formação do canal de Müller. Os tipos de cerdas presentes nos tufos de cerda associados ao canal de Müller são os mesmos encontrados em outros pereópodes e no bordo da região branquiostegal (cerdas simples, paposas e papo-serradas). A única exceção são as cerdas constritas presentes, exclusivamente, nos tufos de cerdas das espécies da família Ocypodidae. Nos tufos de Grapsoidea, a inserção de cerdas no coxopodito do pereópode 4 ocorre somente na sua porção ventral enquanto, em Ocypodoidea, as cerdas do coxopodito do pereópode 4 se inserem tanto na porção ventral como na porção distal. A calha apendicular pode ser formada entre as coxas dos pereópodes 5/6 ou 6/7, de formato cilíndrico ou semicilíndrico. A função da calha apendicular está associada com a condução da água entre o tufo de cerdas e o canal pleural, embora existam casos de formação do canal de Müller sem a presença da calha apendicular. O canal pleural pode ser aberto ou semitubular, ocorrendo, sempre, entre os pleuritos torácicos 6 e 7 e, em algumas espécies, também entre os pleuritos torácicos 5 e 6. O canal pleural aberto é formado pelo afastamento de dois pleuritos torácicos adjacentes; o canal semitubular é formado pela projeção do pleurito 7 sobre o pleurito torácico 6. Foram encontrados, pelo menos, sete padrões de organização do canal de Müller que variam entre a ausência completa da estrutura até a formação de um canal completo (que apresente tufo de cerdas, calha apendicular e canal pleural, concomitantemente). Ucides cordatus (Ucididae) apresentou modificações esqueletais singulares, bastante diferentes dos demais Ocypodoidea e também de Grapsoidea. As espécies que apresentam maior nível de terrestrialidade (família Gecarcinidae) foram as únicas que não apresentaram canal de Müller ou qualquer adaptação para captação de água do substrato. / The conquest of terrestrial environment by brachyuran crabs depends on the attainment and maintainability of water in the branchial chamber during the emersion period. One way to get environmental water is through tufts of hydrophilic setae, capable of picking up water from the soil by capillarity. It is known that these hydrophilic setae are attached to the Müllers opening and the collected water is admitted into the branchial chamber due to the pressure difference between the branchial chamber and the external environment, produced by the scaphognathite beating. However, the morphological modifications of the axial and appendicular skeleton to form the Müllers opening and the composition of the seta tuft are not known. In order to elucidate such gaps, the axial skeleton, the appendicular skeleton and the chaetotaxy of 22 species of semiterrestrial crabs belonging to superfamilies Grapsoidea (Grapsidae, Sesarmidae, Varunidae and Gecarcinidae) and Ocypodoidea (Ocypodidae and Ucididae) were studied. The study of the axial and appendicular skeleton revealed that the Müllers opening is actually a complex channel that may be comprised of three components: the pleural channel, the appendicular gutter and the setal tuft. The water is collected from the substrate by the setal tuft and carried through the appendicular gutter. Then it passes through the pleural channel to be finally admitted into the branchial chamber. The setal tuft is formed between the posterior face of the coxopodite of pereopod 3 and the anterior face of the coxopodite of pereopod 4. In Sesarmidae species, a second tuft also occurs between the coxopodites of pereopods 2 and 3. Representatives of the superfamilies Grapsoidea and Ocypoidea differ in the formation of Müllers channel. In Grapsoidea tuft, the setal insertion occurs only in the ventral portion of coxopodite of pereopod 4 while in Ocypodoidea, the insertion of these seta occurs both in the ventral and distal portions of coxopodite of pereopod 4. The types of seta which are present in setal tufts associated with the Müllers channel are the same found in other pereopods and in the edge of branchiostegal region (simple, paposes and paposerrate seta). The only exception is the constricted seta which is present exclusively in the setal tufts of Ocypodidae species. The appendicular gutter may be formed between the coxopodites of the 5/6 or 6/7 pereopods, in cylindrical or semi-cylindrical shape. The function of the appendicular gutter is associated with the water conduction between the setal tuft and the pleural channel, although there are cases in which the formation of Müllers channel happens without the presence of the appendicular gutter. Pleural channel can be opened or semitubular. They always occur between thoracic pleurites 6/7 and, in some species, between the thoracic pleurites 5 and 6 as well. The open pleural channel is formed by the spacing of two adjacent thoracic pleurites; the semitubular pleural channel is formed by the projection of pleurite 7 above pleurite 6. At least seven patterns of organization of the Müllers channel were found, ranging from the complete absence of the structure to the formation of a full channel (presenting setal tuft, appendicular gutter and pleural channel concurrently). Ucides cordatus (Ucididae) showed quite unique skeletal modifications, different from the others Ocypodoidea and also from the Grapsoidea. The species with the highest level of terrestriality (Gecarcinidae family) were the only ones who did not have Müllers channel or any adaptation to water abstraction from the substrate.

Trata do desenvolvimento de uma metodologia para parametrização de equipamentos mecânicos pressurizados. Objetiva criar sistemas para geração de famílias de equipamentos petroquímicos e reduzir o tempo total de elaboração do projeto, desenhos e listas de materiais em até 80%, diminuir em até 70% o tempo consumido na geração de fichas de corte e mapas de solda na execução de roteiros de fabricação e ainda aprimorar a aquisição de materiais e componentes devido à padronização das entradas de projeto. Relata que os sistemas CAD (Computer-Aided Design) tridimensionais paramétricos têm papel cada vez mais destacado no desenvolvimento de equipamentos, porém alguns setores tradicionais da indústria brasileira apresentam ainda certa resistência ao emprego desta tecnologia. Demonstra que diante dos desafios impostos pela exploração de petróleo na camada do Pré-Sal e o grande volume de investimentos da PETROBRAS na expansão do setor produtivo ligado à extração e refino de petróleo, as empresas fornecedoras de equipamentos para o setor petroquímico são obrigadas a se modernizar e buscar novos paradigmas de projeto e fabricação para atender à demanda. Explica que o emprego produtivo de sistemas CAD 3D paramétricos se torna essencial neste cenário. Aplica a metodologia que foi desenvolvida baseada na abordagem top-down para modelagem de montagens, e afirma esta metodologia pode ser adaptada a qualquer sistema CAD 3D paramétrico comercial, com baixo consumo de recursos computacionais. Apresenta para validação um exemplo de aplicação passo a passo da metodologia para um equipamento. Mostra que a metodologia foi aplicada também para a construção de uma família de vasos de pressão verticais suportados por saia, com o intuito de avaliar o desempenho, com a obtenção de resultados significativos. Discute os problemas e dificuldades enfrentados na implantação do sistema paramétrico, juntamente com as alterações que este sistema trás na estrutura organizacional tradicional de empresas de bens de capital. Conclui que a metodologia desenvolvida apresentou resultados dentro dos objetivos estabelecidos e é uma alternativa viável para projetos mecânicos, que possibilita ganho de produtividade significativo. / This work addresses a methodology for the parameterization of pressurized mechanical equipments. It focuses on creating systems to generate an assembly of petrochemical equipments, hence reducing the total time of project designs, layouts and bill of material until 80%, decreasing until 70% the time consumed for executing manufacturing instructions and also improving materials and components acquisition due to the standardization of project entries. The study reports that three-dimensional parametric CAD systems (Computer-Aided Design) have an increasingly prominent role in the development of equipments, however some traditional sectors in the Brazilian industry are still somewhat resistant to using this technology. This work shows that with the challenges posed by oil exploration in the pre-salt layer and PETROBRAS large investments in the productive expansion sector for oil extraction and refining, the equipment supply companies servicing the petrochemical industry are forced to modernize and seek new design and manufacturing paradigms to meet the demands. It explains that the productive use of 3D parametric CAD systems are essential in this scenario. The methodology that was developed based on the top-down approach for assembly modeling is applied, thereby showing that this methodology can be adapted to any commercial 3D parametric CAD system, with low computational consumption of resources. For validation, it shows a step by step application example of the methodology in one equipment. It shows that the methodology was also applied to the construction of a family of skirt supported vertical pressure vessels, in order to evaluate the performance, with significant results. This work discusses the problems and difficulties faced in implementing the parametric system, along with the changes this system brings in the traditional organizational structure of capital goods companies. It concludes that this methodology presented results within the stated objectives and that it is a viable alternative for mechanical designs, hence enabling significant productivity gains.