De par leurs bonnes propriétés mécaniques, leur faible densité, leur faible coût, et leur bel aspect de surface, les composites SMC (Sheet Molding Compound) sont des matériaux de choix pour réaliser des pièces automobiles semi-structurelles. Ces matériaux sont principalement constitués d’une résine polyester (UPR), d’un additif thermoplastique comme agent anti-retrait, de carbonate de calcium comme charge et de fibres de verre coupées comme renfort. Le contexte environnemental et socio-économique actuel encourage les constructeurs automobiles à utiliser des matériaux issus de ressources renouvelables afin de réduire l’utilisation des réserves pétrolières, et de trouver une alternative au styrène (COV, polluant atmosphérique dangereux et potentiellement cancérigène) qui est utilisé comme diluant réactif (DR) dans les UPR. L’objectif de ce travail est de développer une matrice organique biosourcée (UPR et agent anti-retrait) pour composite SMC moulable par le même procédé de mise en forme et présentant le même niveau de performance comparé à l’existant pétrosourcé. La première partie de ce travail est consacrée à l’évaluation de nouveaux diluants réactifs biosourcés (MMA, BDDMA, BDDVE, EDI, IBOMA et LMA) comme substituants du styrène sur la base des contraintes liées au procédé SMC. Les résines sélectionnées ont ensuite été polymérisées et les réseaux obtenus caractérisés. Le mécanisme de copolymérisation a été étudié et a permis de mettre en évidence l’influence de la nature chimique des insaturations et de la fonctionnalité du DR sur les propriétés finales du réseau. De par sa faible viscosité, sa faible volatilité et son point éclair élevé, le BDDMA est un bon candidat pour remplacer le styrène dans les UPR. La partie suivante est consacrée à la caractérisation d’un prépolymère polyester insaturé et de différents agents anti-retrait biosourcés. Des matrices organiques ont été formulées avec différents agents anti-retrait, puis polymérisées et caractérisées. L’influence de la nature et du taux d’agent anti-retrait sur le retrait de polymérisation et les propriétés mécaniques a été évaluée. Des essais sur composites SMC à l’échelle pilote ont été réalisés afin d’étudier les propriétés finales des composites biosourcés. La meilleure compensation du retrait est obtenue pour l’additif de plus faible Tg, c'est-à-dire le polyester saturé biosourcé. Le réseau à base de BDDMA étant très fragile, la suite de ce travail a consisté à réduire la densité de réticulation du réseau en introduisant un monométhacrylate biosourcé dans la formulation (MMA, IBOMA et LMA) afin d’améliorer les propriétés au choc du matériau. Enfin, un nouveau DR biosourcé, le lévulinate de vinyle, a été évalué comme substituant du styrène dans les UPR. Le mécanisme de copolymérisation a été mis en évidence et relié à la structure et aux propriétés finales du réseau. / Due to their good mechanical properties, low density, low cost and good surface properties, SMC composites (Sheet Molding Compound) are suitable for manufacturing half-structural automotive parts. These materials are mainly based on a unsaturated polyester resin (UPR), thermoplastics as low profile additives, calcium carbonate as filler and chopped glass fibers. Current environmental and socio-economic concerns motivate automotive manufacturers to use bio-based materials in order to reduce the use of crude oil reserves and to find an alternative to styrene (VOC, hazardous air pollutant, potential carcinogen) which is used as reactive diluents (RD) in UPR. The aim of this work was to develop a bio-based organic matrix (UPR and low profile additive) for SMC composites with a similar processability and same level of performances compared to petroleum-based analogs. The first part of this work describes the evaluation of new bio-based reactive diluents (MMA, BDDMA, BDDVE, EDI, IBOMA and LMA) as styrene substituents for UPR, taking into account SMC process requirements. Selected resins were polymerized and the obtained networks characterized. Copolymerization mechanism was studied and allowed to highlight the influence of the chemical nature of unsaturations and the functionality of reactive diluents on network properties. Due to its low viscosity, low volatility and high flashpoint, BDDMA is a good candidate to replace styrene in UPR. Next part was dedicated to the characterization of bio-based unsaturated polyester and low profile additives. Several organic matrices were formulated with different low profile additives, then polymerized and characterized. The influence of the low profile additive nature and content on the polymerization shrinkage and mechanical properties of the material was evaluated. SMC composites were manufactured at the pilot scale and characterized in order to study the final properties of bio-based composites. The best shrinkage control is obtained with low-Tg additive (bio-based saturated polyester). Nevertheless BDDMA-based network is very brittle, so a next step of our work was to introduce a monofunctional methacrylate (MMA, IBOMA and LMA) into the matrix in order to decrease the crosslink density of the network and improve its impact resistance. The influence of the methacrylate nature and content on the structure and mechanical properties of the polyester networks was highlighted. Finally, a new bio-based RD, vinyl levulinate, was evaluated to replace styrene in UPR. Its copolymerization mechanism with UP was studied and related to the structure and mechanical properties of the network.

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Recruitment of mural cells, i.e. pericytes and smooth muscle cells (SMC), is essential to improve the maturation of newly formed vessels. One of the major factors involved in this process is the endothelial cell-secreted Platelet-Derived Growth Factor BB (PDGF BB). Sonic hedgehog (Shh) has also been suggested to promote the formation of larger and more muscularized vessels, but the underlying mechanisms involved have not yet been elucidated. We first identified Shh as a target of PDGF BB and found that SMC respond to Shh not only by upregulating the Gli1-dependent canonical pathway, but also by activating ERK1/2 and PI3K-dependent non-canonical pathways. Moreover, we found that PDGF BB-induced SMC migration, involves Shh-dependent PI3K, ERK1/2 and Gli1 activation. In the mouse model of corneal angiogenesis, PDGF BB and Shh were expressed by endothelial cells and mural cells of VEGF-induced newly formed blood vessels, respectively. PDGF BB inhibition reduced Shh expression, confirming that Shh is a target of PDGF BB, as demonstrated by in vitro experiments. Finally, we found that inhibition of either PDGF BB or Shh signaling reduced NG2+ mural cell recruitment into neovessels and subsequently reduced the neo-vessel lifespan. In this work, we demonstrate, for the first time, that Shh is a key mediator of PDGF BB-induced mural cell migration and recruitment into neo-vessels and elucidates the molecular signaling pathway involved in this process. / Recruitment of mural cells, i.e. pericytes and smooth muscle cells (SMC), is essential to improve the maturation of newly formed vessels. One of the major factors involved in this process is the endothelial cell-secreted Platelet-Derived Growth Factor BB (PDGF BB). Sonic hedgehog (Shh) has also been suggested to promote the formation of larger and more muscularized vessels, but the underlying mechanisms involved have not yet been elucidated. We first identified Shh as a target of PDGF BB and found that SMC respond to Shh not only by upregulating the Gli1-dependent canonical pathway, but also by activating ERK1/2 and PI3K-dependent non-canonical pathways. Moreover, we found that PDGF BB-induced SMC migration, involves Shh-dependent PI3K, ERK1/2 and Gli1 activation. In the mouse model of corneal angiogenesis, PDGF BB and Shh were expressed by endothelial cells and mural cells of VEGF-induced newly formed blood vessels, respectively. PDGF BB inhibition reduced Shh expression, confirming that Shh is a target of PDGF BB, as demonstrated by in vitro experiments. Finally, we found that inhibition of either PDGF BB or Shh signaling reduced NG2+ mural cell recruitment into neovessels and subsequently reduced the neo-vessel lifespan. In this work, we demonstrate, for the first time, that Shh is a key mediator of PDGF BB-induced mural cell migration and recruitment into neo-vessels and elucidates the molecular signaling pathway involved in this process.

Les cellules musculaires lisses (CML) vasculaires les composants cellulaires principaux de la paroi artérielle, sont exposées constamment aux contraintes mécaniques. Les contraintes mécaniques cycliques régulent de nombreuses fonctions des CML vasculaires via les intégrines. Parmi les intégrines, l'[alpha]v[gamma]3 est non seulement un mécano-transducteur mais aussi le récepteur de la prothrombine à la surface des CML. L?activation de l'intégrine [alpha]v[gamma]3 par les contraintes mécaniques pourrait favoriser l'adhésion des CML à la prothrombine et aussi accélérer la génération de thrombine à la surface des CML. Pour vérifier cette hypothèse, nous avons étudié l'effet des contraintes mécaniques sur la génération de thrombine par les CML et identifié les voies de la signalisation impliquées. Nous avons utilisé un modèle de Flexcell utilisant les CML aortiques de rat, soumises à un étirement cyclique (10%, 1Hz). L'exposition à l'étirement cyclique pendant 1h et 6h induit un phénotype de différenciation et non-apoptotique des CML et une augmentation de l'expression de l'intégrine [alpha]v[gamma]3. Il y a aussi une augmentation de la phosphorylation de Src, FAK, AKT de façon temps dépendant et une augmentation de la phosphorylation de l'ILK à 15 min et du clivage de taline de 5 à 60 min. L'étirement cyclique augmente l'adhésion des CML à la prothrombine et la génération de thrombine avec un effet maximum à 6h de 67% et 30% respectivement. Le peptide mimétique de l'intégrine [alpha]v[gamma]3 (cRGDPV) et le siARN [alpha]v bloquent tous les effets de l'étirement cyclique sur les CML. Le siARN taline inhibe l'expression de la sous-unité [alpha]v et également la phosphorylation de Src, AKT et ILK. Le siARN ILK n'a pas d'effet sur l'expression de l'[alpha]v mais inhibe la phosphorylation d'AKT et le clivage de taline à 6h de l'étirement cyclique. Ainsi, l'étirement cyclique induit une plus forte génération de thrombine par les CML vasculaires via l'activation des voies de signalisation dépendante de l'[alpha]v[gamma]3. Cette étude suggère que la génération de thrombine intravasculaire peut être régulée par des antagonistes de l'intégrine [alpha]v[gamma]3 et peut devenir une nouvelle cible thérapeutique chez les patients avec une pression pulsée élevée / Vascular smooth muscle cells (SMC), the main cellular components of the arterial wall, are constantly exposed to mechanical stretch. Cyclic mechanical stress regulates many functions of vascular SMC via integrins. Among the integrins, [alpha]v[gamma]3 is not only a mechanotransducer but also the receptor of prothrombin in the vascular SMC. Activation of integrin [alpha]v[gamma]3 by mechanical stretch may promote SMC adhesion to prothrombin and also accelerate thrombin generation on the surface of SMC. To test this hypothesis, we have studied the effect of mechanical stretch on the generation of thrombin by SMC and identified possible signaling pathway involved. We used a Flexcell model using rat aortic SMC subjected to cyclic stretch (10%, 1Hz). Exposure to cyclic stretch for 1h and 6h induced a phenotype of differentiation and non-apoptosis of SMC and an increased expression of integrin [alpha]v[gamma]3. There was also an increase in phosphorylation of Src, FAK, and AKT in a time dependent manner, increased phosphorylation of ILK at 15min and the cleavage of talin from 5 to 60min. Cyclic stretch increased the adhesion of prothrombin to the SMC, and thrombin generation with a maximum effect of 67% and 30% respectively. A peptide mimetic of integrin [alpha]v[gamma]3 (cRGDPV) and [alpha]v siRNA both blocked all the effects of cyclic stretch on SMC. A talin siRNA inhibited the expression of [alpha]v and the phosphorylation of Src, AKT and ILK. An ILK siRNA has no effect on the expression of [alpha]v but inhibited the phosphorylation of AKT and the cleavage of talin at 6h of stretch. Thus, cyclic stretch induced a higher thrombin generation by vascular SMC via activation of signaling pathways dependant on [alpha]v[gamma]3. This study suggests that intravascular thrombin generation can be regulated by antagonists of integrin [alpha]v[gamma]3 and can become a new therapeutic target for the patients with a high pulse pressure

Nous avons utilisé une technique récente de mesure d'élasticité de chromosomes mitotiques assemblés in vitro de Xénope et mis au point une technique de fonctionnalisation par anticorps de micropipettes. Nous avons confirmé que le chromosome mitotique n'était pas un objet homogène. Il est constitué de deux parties bien distinctes : une gaine molle de chromatine et une structure rigide. Nous avons identifié les protéines SMC comme actrices de ces structures rigides. Nous avons aussi montré que l'ADN et les protéines sont nécessaires au maintien de l'organisation des chromosomes alors que l'ARN ne l'est pas. Nous avons aussi étudié le paysage énergétique du chromosome, ce qui n'avait jamais été réalisé sur un objet aussi complexe. Nous proposons un modèle dynamique de formation des chromosomes dans lequel les protéines SMC agissent comme médiatrices de la forme en bâtonnet des chromosomes mitotiques et dans lequel les ions Mg++ et Ca++ jouent le rôle d'agents de

The Magellanic Clouds (MCs) consist of a pair of galaxies, the Large Magellanic Cloud (LMC) and the Small Magellanic Cloud (SMC), which are located at a distance of 50 kpc and 60 kpc, with stellar masses of 1010 M and 109 M , respectively. Morphologically they are categorized as irregular type galaxies. The MCs are gas rich and metal poor (Z=0.008 for LMC, and 0.004 for SMC) as compared to the Milky Way (MW), and have active star-forming regions. Their proximity and location at high galactic latitude enable us to resolve their individual populations as well as detect faint stellar populations. It is well known that the MCs are interacting with each other, as well as with the MW. The interaction is supported by the presence of the Magellanic Bridge and the Magellanic Stream. The evolved stellar populations in the MCs help us to understand their evolution and interaction process. The MCs host both Population I as well as Population II stars. This extended range of star formation is a valuable source of information to understand the formation and evolution of galaxies in general, and the MCs in particular. Evolved stellar popu-lation means the stars that have evolved o the main sequence and the giants, such as red giants (RGs), red clump stars, and asymptotic giant branch stars. There is a dominant population of evolved stars present in the MCs, in star clusters as well as in the eld. The aim of the thesis is to study the evolved stellar populations for one of the component of the MCs, the LMC. The study is primarily divided into two parts. (1) Study of sparse star clusters in the LMC: To increase our understanding of sparse star clusters in the LMC, with well estimated parameters, using deep Washington photometric data for 45 LMC clusters. (2) To estimate a metallicity map of LMC: In order to understand the metallicity variation across the galaxy. This is done by creating a high spatial resolution metallicity map of the LMC, using red giant branch (RGB) stars, with the help of photometric data and calibrated using spectroscopic studies of RGs in eld and star clusters. The introduction to the thesis study along with the aim are described in Chapter 1 of the thesis. The three sets of photometric data used for this study are described in Chapter 2. The data sets are: CT1 Washington photometric data for 45 star clusters within the LMC, the VI photometric data from the Optical Gravitational Lensing Experiment Phase-III survey (OGLE III), and the Magellanic Cloud Photometric Survey (MCPS). Study of sparse star clusters in the LMC: A systematic study is per-formed to analyse the 45 cluster candidates, to estimate their parameters (radius, reddening, and age) using the main-sequence turn-o (MSTO), as well as the evolved portion of the colour{magnitude diagram (CMD). The basic parameters were estimated for 33 genuine clusters, whereas the other 12 cluster candidates have been classi ed as possible clusters/asterisms. The study of 33 star clusters are presented in Chapter 3. These clus-ters are categorized as genuine star clusters based on their strong density enhancement and cluster features with respect to their surrounding eld regions. Out of the 33 clusters, 23 are identi ed as single clusters and 10 are found to be members of double clusters. Detailed discussions of all the individual clusters are presented. The estimated parameters for the single and double clusters are listed in two di erent tables. About 50% of the clusters are in the age range 100{300 Myr, the rest of them being older or younger. Comparison with previous age estimates shows some agreement as well as some deviation. The remaining 12 clusters which could not be categorized as genuine star clusters are studied in Chapter 4. These clusters have poor (/suspi-cious) density enhancement and cluster features when compared to their surrounding elds. It is important to study such cluster candidates, as these objects probe the lower limit of the cluster mass function. Detailed discussion on these individual objects are presented and their estimated parameters are tabulated in this chapter. A detailed discussion based on the study of all the 45 inconspicuous clusters is presented in this chapter, including the estimated sizes (radii 2{10 pc), reddening with respect to eld, and location in the LMC. The mass limit estimated for genuine clusters is found to be 1000 M , whereas for possible clusters/asterisms it is few 100 M , using synthetic CMDs. The study of sparse clusters enlarged the number of objects con rmed as genuine star clusters (33) and estimated their fundamental parameters. The study emphasizes that the sizes and masses of the studied sample are found to be similar to that of open clusters in the MW. Thus, this study adds to the lower end of cluster mass distribution in the LMC, suggesting that the LMC, apart from hosting rich clusters, also has formed small, less massive open clusters in the 100{300 Myr age range. The 12 cases of possible clusters/asterisms are worthy of attention, in the sense that they can throw light on the survival time of such objects in the LMC. Photometric metallicity map of the LMC using RGB stars: A metallic-ity map of the LMC is estimated using OGLE III and MCPS photometric data. This is a rst of its kind map of metallicity up to a radius of 4{5 de-grees, derived using photometric data and calibrated using spectroscopic data of RGB stars. The RGB is identi ed in the V, (V I) CMDs of small areal subregions of varying sizes in both data sets. The slope of the RGB is used as an indicator of the average metallicity of a subregion, and this RGB slope is calibrated to metallicity using spectroscopic data for eld and cluster RGs in selected subregions. The metallicity map estimated using OGLE III photometric data is presented in Chapter 5. A method to identify the RGB of small subre-gions within the LMC and estimate its slope by using a consistent and automated method was developed. The technique is robust and indepen-dent of reddening and extinction. The details of calibrating the RGB slopes to metallicities, using previous spectroscopic results of RGs in eld and star clusters are presented. The OGLE III metallicity maps are pre sented, based on four cut-o criteria to separate regions with good ts. The OGLE III map has substantial coverage of the bar, the eastern and western LMC, but does not cover the northern and southern regions. The OGLE III metallicity map shows the bar region to be metal rich whereas the eastern and western regions to be relatively metal poor. The mean metallicity is estimated for three di erent regions within the LMC. For the complete LMC the mean [Fe/H] is = 0.39 dex ( [Fe/H] = 0.10); for the bar region it is = 0.35 dex ( [Fe/H] = 0.9); and for the outer LMC it is = 0.46 dex ( [Fe/H] = 0.11). The metallicity histogram for these di erent regions are also estimated. A radial metallicity gradient is estimated in the de-projected plane of the LMC. The metallicity gradient is seen to remain almost constant in the bar region (till a radius of 2.5 kpc) and has a shallow gradient of 0.066 0.006 dex kpc 1 beyond that till 4 kpc. In Chapter 6 the metallicity map based on MCPS photometric data is estimated. The MCPS data covers more of the northern and south-ern LMC (less of eastern and western regions) and is important to be analysed in order to reveal the metallicity trend of the overall disk. The systematic di erences between the lter systems of MCPS and OGLE III are corrected, and the MCPS slopes are then calibrated using the OGLE III slope{metallicity relation. The MCPS metallicity maps are presented, based on four cut-o criteria to separate regions with good ts. The bar region is found to be metal rich as was found using OGLE III data, whereas the northern and southern regions are marginally metal poor. The mean metallicity estimated for the complete LMC is = 0.37 dex ( [Fe/H] = 0.12); and for the outer LMC it is = 0.41 dex ( [Fe/H] = 0.11). The metallicity histogram for these di erent regions are estimated and compared with the OGLE III distribution. The metallicity range of the complete LMC is found to be almost similar for both data sets. The metallicity distribution within the bar has a narrow range as found using both data sets. The slight di erence between mean metallicity of outer LMC for the two data sets is attributed to their coverage. We suggest that the northern and southern regions of the LMC could be marginally more metal rich than the eastern and western regions. The metallicity gradient of the LMC disk, estimated from MCPS data is found to be shallow 0.049 0.002 dex kpc 1 till about 4 kpc. We also constructed a metallicity map of outliers using both OGLE III and MCPS data, and identi ed subregions where the mean metallic-ity di ers from the surrounding areas. We suggest further spectroscopic studies in order to assess their physical significance. The detailed conclusion of the thesis and future work are presented in Chapter 7. From the study of sparse star clusters in the LMC, it is concluded that LMC has open cluster like star cluster systems. It is important to include them to understand the cluster formation history (CFH) and their survival time scale. Presently, our understanding of the CFH is dominated by rich clusters. The bar of the LMC is found to be the most metal rich region, and the LMC metallicity gradient though shallow, resembles the gradient seen in spiral galaxies. The gradient is also similar to that found in our Galaxy. The higher metallicity in the bar region might indicate an active bar in the past.

Structural Maintenance of Chromosomes (SMC) proteins are a highly conserved class of proteins required for the maintenance of genome stability and regulate nearly all aspects of chromosome biology. Eukaryotes, such as the budding yeast Saccharomyces cerevisiae, have six Smc proteins that form three SMC complexes in association with non-SMC proteins, i.e., the cohesin complex, the condensin complex and the Smc5/6 complex. The yeast Smc5/6 complex consists of Smc5, Smc6 and six non-Smc elements (Nse1-6) that are all essential for the survival of cells. Nse1 is the first non-smcelement that was identified associated with the Smc5/6 complex. Nse1 has a C-terminal RING-domain, which is a characteristic feature of some E3 ubiquitin ligases. A RING domain consists of eight conserved Zn-coordinating residues arranged in a cross-brace conformation. To understand the importance of this domain, we created site directed mutations in conserved residues identified by sequence alignment of the budding yeast Nse1 RING domain with that of other species. We found a new RING domain mutant nse1-103that was temperature sensitive at 37°C and showed an increased sensitivity towards genotoxic agents such as hydroxyurea (HU), methyl methane sulfonate (MMS) and ultraviolet (UV) radiation. Thense1-103 mutant cells are slow growing and show delayed chromosomal replication at the restrictive temperature. Genetic interactions with replication factors such as RRM3, TOF1 etc. revealed thatnse1-103shows a synthetic sick growth defect in combination with rrm3∆ that is partially suppressed by deletion of TOF1. We found an enhancement in chromosome loss in nse1-103 compared to wild type cells. This was accompanied by a slight reduction in cohesion between the sister chromatids in nse1-103,suggesting a plausible mechanism for the chromosome destabilization observed in the mutant. Since Nse1 forms part of a trimeric sub-complex with Nse3 and Nse4 in the Smc5/6 complex, we performed a yeast two hybrid assay to test the interaction of nse1-103 with Nse3 or Nse4, and found a defect in interaction of nse1-103 with Nse3 and Nse4. In addition, a defect in association of nse1-103 with Smc5 or Smc6 could be observed by performing co-immunoprecipitation from yeast cell lysates, suggesting that the integrity of the RING-domain is critical for the interaction of Nse1 with other subunits of the Smc5/6 complex. However, there was no defect in the interaction between Nse3 and Smc5 in nse1-103, indicating that the interaction of these components within the complex isindependent of Nse1. We also identified a novel sequence motif near the RING domain of Nse1, deletion of which leads to an increased sensitivity towards genotoxic stressors and higher temperature. Biochemical characterization of this mutant also suggests a defect ininteraction with Nse3 or Nse4, and also with Smc5. The nse1 mutants also showed defects in post translational modification of Smc5 and other proteins. Since the Smc5/6 complex also has a SUMO E3 ligase, Mms21/Nse2, we also investigated genetic interactions between the RING domain mutant,nse1-103 and the SUMO ligase RING domain defective mutant,mms21∆sl, and found an exacerbation of the drug sensitive phenotypes in thense1-103 mms21∆sl double mutant relative to either of the single mutants nse1-103 or mms21∆sl, indicating that the two proteins contribute independently to the function of Smc5/6 complex in resisting genotoxic stress. In conclusion, the present study emphasizes the role of the RING domain of budding yeast Nse1 in resisting genotoxic stress and maintaining chromosome stability and reveals that the integrity of the RING-domain is critical for interactions of Nse1 with Nse3 and other Smc5/6 complex components. In addition, we report identification of another novel sequence motif in Nse1 that is also crucial for its interaction with other subunits of the Smc5/6 complex and for maintenance of post-translational modifications of some cellular proteins.

This thesis evaluates different controllers for traction control on an electric forklift truck and has been done in cooperation with Toyota Material Handling Manufacturing Sweden. The need for a traction control system has increased with the introduction of lithium-ion batteries replacing the older lead-acid batteries, reducing the battery weight and therefore the downward force on the driving wheel increasing the risk for slip. The forklift truck was modelled using Simulink and validated by experiment. Different possible control strategies were investigated and three were chosen for implementation in simulation. These were controllers based on Model Following Control, Maximum Transmissible Torque Estimation and Sliding Mode Control. Model Following Control makes use of a nominal model to compare actual wheel speed values with nominal wheel speed values to determine if slip is occurring, Maximum Transmissible Torque Estimation makes use of a closed-loop disturbance observer to compute the maximum transmissible torque possible without inducing slip and using it as a limitation on the input signal, and Sliding Mode Control uses different functions to \say{slide} along a sliding surface to stay around a specific slip value. All three controller types were developed both as speed controlled and torque controlled. All of the controllers could reduce slip heavily in simulation. The Maximum Transmissible Torque Estimation controller reduced slip the most and kept oscillations at a minimum, but was not as responsive as the others to driver commands. The conclusion was that the controller of choice would depend on the working environment of the forklift truck. In a low friction environment where slip is expected to occur often, the Maximum Transmissible Torque Estimation controller is advisable, while the other two would be a better choice for environment with low slip occurrence. The use of torque control, while often better with regards to decreasing slip, could not be advised due to a perceived increase in implementation cost.

Yes / The proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76

Orientador: Aneirson Francisco da Silva / Resumo: A Estampagem a Frio é um processo de conformação plástica de chapas metálicas, que possibilita, por meio de ferramentas específicas, obter componentes com boas propriedades mecânicas, geometrias e espessuras variadas, diferentes especificações de materiais e com boa vantagem econômica. A multiplicidade destas variáveis gera a necessidade de utilização de técnicas estatísticas e de simulação numérica, que suportem a sua análise e adequada tomada de decisão na elaboração do projeto das ferramentas de conformação. Este trabalho foi desenvolvido em uma empresa brasileira multinacional de grande porte que atua no setor de autopeças, em seu departamento de engenharia de projetos de ferramentas, com o propósito de reduzir o estiramento e a ocorrência de trincas em uma travessa de 6,8 [mm] de aço LNE 380. A metodologia proposta obtém os valores dos fatores de entrada e sua influência na variável resposta com o uso de técnicas de Delineamento de Experimentos (DOE) e simulação pelo método de Elementos Finitos (FE). Uma Função Empírica é desenvolvida a partir desses dados, com o uso da técnica de regressão, obtendo-se a variável resposta y (espessura na região crítica), em função dos fatores influentes xi do processo. Com a Otimização via Simulação Monte Carlo (OvSMC) insere-se a incerteza nos coeficientes desta Função Empírica, sendo esta a principal contribuição deste trabalho, pois é o que ocorre, por via de regra, na prática com problemas experimentais. Simulando-se por FE as ferram... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre