<p>Methane (CH₄) is the second most powerful greenhouse gas (GHG) behind carbon dioxide (CO₂), and is able to trap a large amount of long-wave radiation, leading to surface warming. Carbon monoxide (CO) plays an important role in controlling the oxidizing capacity of the atmosphere by reacting with OH radicals that affect atmospheric CH₄ dynamics. Terrestrial ecosystems play an important role in determining the amount of these gases into the atmosphere. However, global quantifications of CH₄ emissions from wetlands and its sinks from uplands, and CO exchanges between land and the atmosphere are still fraught with large uncertainties, presenting a big challenge to interpret complex atmospheric CH₄ dynamics in recent decades. In this dissertation, I apply modeling approaches to estimate the global CH₄ and CO exchanges between land ecosystems and the atmosphere and analyze how they respond to contemporary and future climate change.</p> <p>Firstly, I develop a process-based biogeochemistry model embedded in Terrestrial Ecosystem Model (TEM) to quantify the CO exchange between soils and the atmosphere at the global scale (Chapter 2). Parameterizations were conducted by using the CO <i>in situ</i> data for eleven representative ecosystem types. The model is then extrapolated to global terrestrial ecosystems. Globally soils act as a sink of atmospheric CO. Areas near the equator, Eastern US, Europe and eastern Asia will be the largest sink regions due to their optimum soil moisture and high temperature. The annual global soil net flux of atmospheric CO is primarily controlled by air temperature, soil temperature, SOC and atmospheric CO concentrations, while its monthly variation is mainly determined by air temperature, precipitation, soil temperature and soil moisture. </p> <p>Secondly, to better quantify the global CH₄ emissions from wetlands and their uncertainties, I revise, parameterize and verify a process-based biogeochemical model for methane for various wetland ecosystems (Chapter 3). The model is then extrapolated to the global scale to quantify the uncertainty induced from four different types of uncertainty sources including parameterization, wetland type distribution, wetland area distribution and meteorological input. Spatially, the northeast US and Amazon are two hotspots of CH₄ emissions, while consumption hotspots are in the eastern US and eastern China. The relationships between both wetland emissions and upland consumption and El Niño and La Niña events are analyzed. This study highlights the need for more in situ methane flux data, more accurate wetland type and area distribution information to better constrain the model uncertainty.</p> <p>Thirdly, to further constrain the global wetland CH₄ emissions, I develop a predictive model of CH₄ emissions using an artificial neural network (ANN) approach and available field observations of CH₄ fluxes (Chapter 4). Eleven explanatory variables including three transient climate variables (precipitation, air temperature and solar radiation) and eight static soil property variables are considered in developing the ANN models. The models are then extrapolated to the global scale to estimate monthly CH₄ emissions from 1979 to 2099. Significant interannual and seasonal variations of wetland CH₄ emissions exist in the past four decades, and the emissions in this period are most sensitive to variations in solar radiation and air temperature. This study reduced the uncertainty in global CH₄ emissions from wetlands and called for better characterizing variations of wetland areas and water table position and more long-term observations of CH₄ fluxes in tropical regions.</p> <p>Finally, in order to study a new pathway of CH₄ emissions from palm tree stem, I develop a two-dimensional diffusion model. The model is optimized using field data of methane emissions from palm tree stems (Chapter 5). The model is then extrapolated to Pastaza-Marañón foreland basin (PMFB) in Peru by using a process-based biogeochemical model. To our knowledge, this is among the first efforts to quantify regional CH₄ emissions through this pathway. The estimates can be improved by considering the effects of changes in temperature, precipitation and radiation and using long-period continuous flux observations. Regional and global estimates of CH₄ emissions through this pathway can be further constrained using more accurate palm swamp classification and spatial distribution data of palm trees at the global scale.</p>

Biogas im Rahmen einer nachhaltigen Landwirtschaft zu erzeugen bedeutet u. a., die anfallenden Gärprodukte als Dünger zu verwenden, um die Verluste im Nährstoffkreislauf zu minimieren. Die vorliegende Arbeit geht der Frage nach, welche Wirkungen Gärprodukte aus der Biogasproduktion bei der Verwendung als Dünger auf Boden und Pflanzen haben. Die Ergebnisse von Parzellenfeld- und Praxisversuchen, in denen Gärprodukte aus der Mono- und Kofermentation von Energiepflanzen im Vergleich zu N-Mineraldünger untersucht wurden, werden vorgestellt und diskutiert. Zur Beurteilung der Wirkungen auf den Boden wurden bodenchemische und bodenbiologische Parameter herangezogen sowie die Abbaustabilität der organischen Substanz der Gärprodukte gemessen. Zur Untersuchung der Wirkungen auf die Pflanzen wurden verschiedene Wachstums-, Entwicklungs-, Ertrags- und Qualitätsparameter erfasst und ausgewertet. Die Gärprodukte zeichnen sich durch relativ hohe Ammoniumgehalte sowie durch hohe pH-Werte aus. Das Pflanzenwachstum und die Erträge werden durch die Düngung mit Gärprodukten gesteigert, wobei die Wirkung trotz der hohen Ammoniumgehalte deutlich hinter denen des N-Mineraldüngers zurückbleibt. Die berechneten Nährstoffbilanzen weisen auf deutliche Unterschiede zwischen den untersuchten Gärprodukten und Kulturarten, aber auch zwischen den beiden Versuchsjahren hin. Die Ergebnisse zeigen jedoch, dass durch die Verwendung von Gärprodukten als Dünger wichtige Pflanzennährstoffe rezykliert werden können, wodurch der Einsatz von Mineraldüngern reduziert werden kann. Hinsichtlich der Wirkungen von Gärprodukten auf den Boden zeichnen die umfangreichen Analysen ein differenziertes Bild. Die mikrobiologischen Umsetzungsprozesse im Boden werden insbesondere in den ersten Tagen nach der Ausbringung gefördert. Weiterer Forschungsbedarf wird insbesondere hinsichtlich der Wirkungen auf die Bodenmakrofauna aufgezeigt. / Producing biogas in a sustainable agricultural system means using digestates as fertilizers, in order to minimize leaks in nutrient cycles. The aim of this work is to investigate effects on soil and plants after field application of digestates. In this respect, results from plot- and practical fieldexperiments are analyzed to compare digestates from mono- and from co-fermentation of energy crops in comparison to mineral N-fertilizer. Soil chemical and biological effects were assessed on the basis of selected parameters, one of which was the stability of the organic matter applied. Effects on crops have been evaluated by measuring growth, development, yields, and quality of the crops. The digestates have been analyzed and showed especially high amounts of ammonium and a high pH-value. Plant growth and yields increased as a result of fertilization. However, despite high amounts of ammonia present in digestates, fertilizing effects have been much lower compared to mineral N-fertilization. The calculated nutrient balances showed obvious differences between the digestates analyzed, crops, and also between the two experimental years. Generally, the results demonstrate that nutrients can be recycled by using digestates as fertilizers and thus the use of mineral fertilizers can be reduced. The extensive soil analyses presented in this work show diverse results. Microbial metabolic processes in the soil are increased especially during the first few days after digestate application. More research is needed with respect to effects on macro fauna.

Land application of wastes has become increasingly popular, to promote nutrient recycling and environmental protection, with soil functioning as a partial barrier between wastes and groundwater. Dairy shed effluent (DSE), may contain a wide variety of pathogenic micro-organisms, including bacteria (e.g. Salmonella paratyphyi, Escherichia coli. and Campylobacter), protozoa and viruses. Groundwater pathogen contamination resulting from land-applied DSE is drawing more attention with the intensified development of the dairy farm industry in New Zealand. The purpose of this research was to investigate the fate and transport of bacterial indicator-faecal coliform (FC) from land-applied DSE under different irrigation practices via field lysimeter studies, using two water irrigation methods (flood and sprinkler) with contrasting application rates, through the 2005-2006 irrigation season. It was aimed at better understanding, quantifying and modelling of the processes that govern the removal of microbes in intact soil columns, bridging the gap between previous theoretical research and general farm practices, specifically for Templeton soil. This study involved different approaches (leaching experiments, infiltrometer measurements and a dye infiltration study) to understand the processes of transient water flow and bacterial transport; and to extrapolate the relationships between bacterial transport and soil properties (like soil structure, texture), and soil physical status (soil water potential ψ and volumetric water content θ). Factors controlling FC transport are discussed. A contaminant transport model, HYDRUS-1D, was applied to simulate microbial transport through soil on the basis of measured datasets. This study was carried out at Lincoln University’s Centre for Soil and Environmental Quality (CSEQ) lysimeter site. Six lysimeters were employed in two trials. Each trial involved application of DSE, followed by a water irrigation sequence applied in a flux-controlled method. The soil columns were taken from the site of the new Lincoln University Dairy Farm, Lincoln, Canterbury. The soil type is Templeton fine sandy loam (Udic-Ustochrept, coarse loamy, mixed, mesic). Vertical profiles (at four depths) of θ and ψ were measured during leaching experiments. The leaching experiments directly measured concentrations of chemical tracer (Br⁻ or Cl⁻) and FC in drainage. Results showed that bacteria could readily penetrate through 700 mm deep soil columns, when facilitated by water flow. In the first (summer) trial, FC in leachate as high as 1.4×10⁶ cfu 100 mL⁻¹ (similar to the DSE concentration), was detected in one lysimeter that had a higher clay content in the topsoil, immediately after DSE application, and before any water irrigation. This indicates that DSE flowed through preferential flow paths without significant treatment or reduction in concentrations. The highest post-irrigation concentration was 3.4×10³ cfu 100 mL⁻¹ under flood irrigation. Flood irrigation resulted in more bacteria and Br⁻ leaching than spray irrigation. In both trials (summer and autumn) results showed significant differences between irrigation treatments in lysimeters sharing similar drainage class (moderate or moderately rapid). Leaching bacterial concentration was positively correlated with both θ and ψ, and sometimes drainage rate. Greater bacterial leaching was found in the one lysimeter with rapid whole-column effective hydraulic conductivity, Keff, for both flood and spray treatments. Occasionally, the effect of Keff on water movement and bacterial transport overrode the effect of irrigation. The ‘seasonal condition’ of the soil (including variation in initial water content) also influenced bacterial leaching, with less risk of leaching in autumn than in summer. A tension infiltrometer experiment measured hydraulic conductivity of the lysimeters at zero and 40 mm suction. The results showed in most cases a significant correlation between the proportion of bacteria leached and the flow contribution of the macropores. The higher the Ksat, the greater the amount of drainage and bacterial leaching obtained. This research also found that this technique may exclude the activity of some continuous macropores (e.g., cracks) due to the difference of initial wetness which could substantially change the conductivity and result in more serious bacterial leaching in this Templeton soil. A dye infiltration study showed there was great variability in water flow patterns, and most of the flow reaching deeper than 50 cm resulted from macropores, mainly visible cracks. The transient water flow and transport of tracer (Br⁻) and FC were modelled using the HYDRUS-1D software package. The uniform flow van Genuchten model, and the dual-porosity model were used for water flow and the mobile-immobile (MIM) model was used for tracer and FC transport. The hydraulic and solute parameters were optimized during simulation, on the basis of measured datasets from the leaching experiments. There was evidence supporting the presence of macropores, based on the water flow in the post-DSE application stage. The optimised saturated water content (θs) decreased during the post-application process, which could be explained in terms of macropore flow enhanced by irrigation. Moreover, bacterial simulation showed discrepancies in all cases of uniform flow simulations at the very initial stage, indicating that non-equilibrium processes were dominant during those short periods, and suggesting that there were strong dynamic processes involving structure change and subsequently flow paths. It is recommended that management strategies to reduce FC contamination following application of DSE in these soils must aim to decrease preferential flow by adjusting irrigation schemes. Attention needs to be given to a) decreasing irrigation rates at the beginning of each irrigation; b) increasing the number of irrigations, by reducing at the same time the amount of water applied and the irrigation rate at each irrigation; c) applying spray irrigation rather than flood irrigation.

The overall aim of this research was to improve the understanding of the ecology and diversity of Trichoderma species within the soil and rhizosphere of onion (Allium cepa L.) and potato (Solanum tuberosum L.) under intensive management in New Zealand. The indigenous Trichoderma population was measured in a field trial at Pukekohe over a three year period under six different crop rotation treatments. The treatments included two continuous onion and potato rotations (intensive), two onion/potato mixed rotation (conventional), and two green manure rotations (sustainable). Results showed that Trichoderma populations were stable in both the rhizosphere and bulk soil (1.5 x 10² to 8.5 x 10³ CFU g⁻¹ ODS). The planting and incorporation of an oat (Avena sativa L.) green manure in the sustainable rotations positively increased Trichoderma colony forming unit (CFU) numbers in the rhizosphere soil from 3.4 x 10² to 2.5 x 10³ g⁻¹ ODS. A Trichoderma species identification method was developed based on colony morphology. Representative isolates were verified using restriction fragment length polymorphism (RFLP) and DNA sequencing. The method allowed for rapid and reliable identification of isolated Trichoderma species. Five species were identified in the Pukekohe soil: T. asperellum, T. atroviride, T. hamatum, T. harzianum and T. koningii. Results showed identical species diversity between the rhizosphere, rhizoplane and bulk soil. The species did not strongly compete between each other for the rhizosphere ecological niche and differences in species proportions seemed to be caused by environmental factors rather than the rotation treatments. The incorporation of oat green manure in pots did not significantly promote the indigenous Trichoderma population size and diversity in the rhizosphere of onion plants up to 4 months old. The identified species were the same as in the field trial. The incorporation of onion scale residues was shown to result in low Trichoderma and high Penicillium CFU numbers and a reduction in plant size. Additionally, the presence of high levels (6.0 x 10⁵ CFU g⁻¹ ODS) of Penicillium CFU was negatively correlated with the presence of Trichoderma CFU. The effect of oat incorporation on Trichoderma saprophytic growth was also investigated in a soil sandwich assay and revealed no significant differences. A series of experiments indicated that onion extract obtained from dry onion scale residues had no antifungal activity against either Trichoderma or Penicillium and instead tended to promote their hyphal growth and sporulation. It also showed that competition between Penicillium and Trichoderma isolates was limited despite the ability of Penicillium to produce a wide range of inhibitory substances. Four indigenous Trichoderma species (T. atroviride, T. hamatum, T. harzianum and T. koningii) were shown to be rhizosphere competent in a split tube experiment over a 6 week period. The results of this experiment revealed that, the Trichoderma species clearly displayed differences in their ability to colonise the rhizosphere of young onion seedlings. Species such as T. koningii had the greatest rhizosphere colonising ability regardless of soil depth while T. harzianum displayed the weakest ability. Results also indicated that when inoculated as a mixture the four species competed with one another to colonise the rhizosphere. Overall, this research indicated that the studied crop rotation treatments and the use of oat as a green manure did not strongly promote indigenous Trichoderma populations. Species diversity was constant throughout the research with T. hamatum and T. koningii being the most frequently isolated species.

New Zealand’s 5.3 million strong dairy herd returns approximately 106 million litres of urine to pasture soils daily. The urea in that urine is rapidly hydrolysed to ammonium (NH₄⁺), which is then nitrified, with denitrification of nitrate (NO₃⁻) ensuing. Nitrous oxide (N₂O), a potent greenhouse gas (GHG), is produced via nitrification and denitrification, which are enzyme-catalysed processes mediated by soil microbes. Thus microbes are linked intrinsically to urine patch chemistry. However, few previous studies have investigated microbial dynamics in urine patches. Therefore the objective of these four experiments was to investigate the effects on soil microbial communities of cow urine deposition. Methods used included phospholipid fatty acid (PLFA) analyses of microbial community structure and microbial stress, dehydrogenase activity (DHA) assays measuring microbial activity, and headspace gas sampling of N₂O, ammonia (NH₃) and carbon dioxide (CO₂) fluxes. Experiment 1, a laboratory study, examined the influence of soil moisture and urinary salt content on the microbial community. Both urine application and high soil moisture increased microbial stress, as evidenced by significant changes in PLFA trans/cis and iso/anteiso ratios. Total PLFAs and DHA showed a short-term (< 1 week) stimulatory effect on microbes after urine application. Mean cumulative N₂O-N fluxes were 2.75% and 0.05% of the nitrogen (N) applied, from the wet (70% WFPS) and dry (35% WFPS) soils, respectively. Experiment 2, a field trial, investigated nutrient dynamics and microbial stress with plants present. Concentrations of the micronutrients, copper, iron and molybdenum, increased up to 20-fold after urine application, while soil phosphorus (P) concentrations decreased from 0.87 mg kg ⁻¹ to 0.48 mg kg⁻¹. Plant P was also lower in urine patches, but total PLFAs were higher, suggesting that microbes had utilised the available nutrients. Microbial stress again resulted from urine application but, in contrast to experiment 1, the fungal biomass recovered after its initial inhibition. Studies published during the course of this thesis reported that hippuric acid (HA) and its hydrolysis product benzoic acid (BA) significantly reduced N₂O-N emissions from synthetic cow urine, thus experiment 3 investigated this effect using real cow urine. Cumulative N₂O-N fluxes were 16.8, 5.9 and 4.7% of N applied for urine (U) alone, U+HA and U+BA, respectively. Since NH₃-N volatilisation remained unchanged, net gaseous N emissions were reduced. Trends in total PLFAs and microbial stress were comparable to experiment 1 results. Experiment 4 studied HA effects at different temperatures and found no inhibition of N₂O-N fluxes from HA-amended urine. However, mean cumulative N₂O-N fluxes were reduced from 7.6% of N applied at 15–20°C to 0.2% at 5–10°C. Total cumulative N emissions (N₂O-N + NH₃-N) were highest at 20°C (17.5% of N applied) and lowest at 10°C (9.8% of N applied). Microbial activity, measured as potential DHA, increased with increasing temperature. This work has clearly shown that the stimulation and inhibition of the soil microbial community by urine application are closely linked to soil chemistry and have significant impacts not only on soil nutrient dynamics but also on N₂O-N emissions and their possible mitigation.

It is widely accepted that bioavailability, rather than total soil concentration, is preferred when assessing the risk associated with metal contamination. Despite this, debate continues on what constitutes a bioavailable pool and how to best predict bioavailability, especially in relation to crop plants. The overall aim of this thesis was to assess and validate measures of cadmium (Cd), copper (Cu), nickel (Ni) and zinc (Zn) bioavailability in a range of soils amended with metal salts and biosolids. Six potential measures of bioavailability were investigated and compared: total metal; 0.04 M EDTA extraction; 0.05 M Ca(NO₃)₂ extraction; soil solution extracted using rhizon probes; effective solution concentration (CE) determined using diffusive gradients in thin films (DGT); and modelled free ion activities (WHAM 6.0). These were compared to shoot metal concentrations obtained from plants grown in three soils with contrasting properties treated with biosolids and metal salts. The first study involved a wheat seedling (Triticum aestivum) assay carried out under controlled environmental conditions on incubated soils treated with metal salts and biosolids. Results showed that the presence of biosolids resulted in increases of DOC, salinity, Ca and Mg in soil solution as well as total concentrations of Cu and Zn, dry matter was also adversely affected by increased levels of salinity. The addition of biosolids did not significantly alter the extractability or solubility of Cd, Cu, Ni and Zn although concentrations of Cd in shoots were significantly lower in plants grown in biosolids amended soils compared with unamended soils. The second study involved a field experiment that used 20 cm diameter by 30 cm deep soil monoliths of the same three soils treated with metals and biosolids, and perennial ryegrass (Lolium perenne) was grown for 24 months. Results revealed the addition of biosolids significantly increased the amount of DOC, salinity, Ca and Mg in solution. The presence of biosolids also significantly altered the bioavailability of Cd, Cu, Ni and Zn, as measured by soil solution, CE and free ion activity. However, this change had little effect on plant metal uptake. The length of time following treatment application had the greatest effect on soil chemistry and metal availability, resulting in pH decreases and increases in DOC, soil solution salinity, Ca and Mg. The free ion activities of each metal increased with time, as did soil solution Cd and Zn and CE-Cu, with results for Zn indicative of migration through the soil profile with time. Plant uptake of Ni and Zn also changed with time. Nickel concentrations in shoots decreased, while concentrations of Zn in shoots increased. The findings from the two studies demonstrated that biosolids increased the amount of DOC, salinity, Ca and Mg present in soil solution. In the lysimeter study measures of metal availability were affected in soils amended with biosolids, but this did not effect shoot concentrations. The overall predictive strengths of the six potential measures of bioavailability was investigated using results from the previously described experiments and related studies carried out by ESR and Lincoln University using nine different soils amended with combinations of biosolids and metal salts. Of the four metals Ni provided the strongest correlations between metal bioavailability and shoot concentrations, with 0.05 M Ca(NO₃)₂ extraction giving the strongest relationship for Ni concentrations in shoots (r² = 0.73). This suggests that the solubility of Ni is highly indicative of shoot concentrations and that Ca(NO₃)₂ is a robust measure of Ni bioavailability. In addition Ca(NO₃)₂ provided the best estimate of Zn bioavailability (r² = 0.65), and CE-Cd provided the best measure of Cd bioavailability, although it could only describe 47 % of shoot Cd concentration. Results for Cu were typical of previously described studies as assays of Cu availability are almost always poorly correlated with shoot concentrations, with total Cu having the strongest relationship (r² = 0.34). Methods based on the extractability and solubility of Cu in soils were poor indicators of Cu concentration in shoots. Overall, the addition of biosolids did not alter the outcome of these bioavailability assays, and results indicated that total metal concentrations present in the soils and biosolids matrix, plus length of time since soil treatment, had a greater affect on metal bioavailability.

Early in 1962 the Forest Research Institute of the New Zealand Forest Service became aware that stands of second crop Pinus radiata (D. Don) on some areas of the Moutere Gravel formation were showing slow growth and had a chlorotic appearance (Fig. 1.1). The second crop followed clear felling of mature P. radiata trees and were aged from 0 to 15 years (stone and Will, 1965). It was felt that the apparent reduced growth of the second generation had much in common with similar productivity decline reported especially in European forestry literature. Stone and Will (1965) postulated that the immediate cause o£ the decline was a deficiency of nitrogen highlighted by the low levels of nitrogen in the leaves of the second crop trees, especially those growing on ridge sites. Numerous field trials have been laid out but many of the trials were poorly designed and consequently could not provide statistically sound results. However, some indication of nutrient deficiencies which occur on the Moutere Gravels were obtained. Even before these trials were laid down nutrient deficiencies had been highlighted by early attempts at farming. It was the partial failure of these crops that initially led to the planting of exotic pines, in the belief that these trees thrived on a limited supply of nutrients. The first crop of pines generally fulfilled expectations but nutrient deficiencies started to appear in extensive areas of the second crop. Accordingly, the Forest Research Institute made available three scholarships to study different aspects of the problem. Work was started on a study of the soil sequence across the Moutere Gravels to determine if there was a general decline in fertility of tho soil with the age of the soil and the environmental factors, such as climate which differs in the high inland areas and the low seaside areas of the Moutere Gravels, rather than a particular decline in fertility induced by the first crop of P. radiata. In another study the major weed species Ulex europaeus and Cytisus scoparius was studied to see if its value as a nitrogen fixer would outweigh its disadvantages as a silvicultural weed. Thirdly, a study of the microbial ecology of the soils was undertaken. Whyte (1966) reported that the second rotation trees started to increase their growth rate after approximately five years to a level paralleling the estimated growth rate of the first crop. It was postulated that the residues (needles, roots and branches) remaining after clear felling could cause an increase in microbial numbers and activity with a consequent immobilization of mineral nutrients which were not initially very plentiful. For this reason an area in Tasman Forest was selected in which mature trees and regeneration up to nine years old were found together to study microbial activity and numbers, energy dissipation and nitrogen dynamics to determine if immobilization of nutrients was causing the apparent declines.

It is widely accepted that bioavailability, rather than total soil concentration, is preferred when assessing the risk associated with metal contamination. Despite this, debate continues on what constitutes a bioavailable pool and how to best predict bioavailability, especially in relation to crop plants. The overall aim of this thesis was to assess and validate measures of cadmium (Cd), copper (Cu), nickel (Ni) and zinc (Zn) bioavailability in a range of soils amended with metal salts and biosolids. Six potential measures of bioavailability were investigated and compared: total metal; 0.04 M EDTA extraction; 0.05 M Ca(NO₃)₂ extraction; soil solution extracted using rhizon probes; effective solution concentration (CE) determined using diffusive gradients in thin films (DGT); and modelled free ion activities (WHAM 6.0). These were compared to shoot metal concentrations obtained from plants grown in three soils with contrasting properties treated with biosolids and metal salts. The first study involved a wheat seedling (Triticum aestivum) assay carried out under controlled environmental conditions on incubated soils treated with metal salts and biosolids. Results showed that the presence of biosolids resulted in increases of DOC, salinity, Ca and Mg in soil solution as well as total concentrations of Cu and Zn, dry matter was also adversely affected by increased levels of salinity. The addition of biosolids did not significantly alter the extractability or solubility of Cd, Cu, Ni and Zn although concentrations of Cd in shoots were significantly lower in plants grown in biosolids amended soils compared with unamended soils. The second study involved a field experiment that used 20 cm diameter by 30 cm deep soil monoliths of the same three soils treated with metals and biosolids, and perennial ryegrass (Lolium perenne) was grown for 24 months. Results revealed the addition of biosolids significantly increased the amount of DOC, salinity, Ca and Mg in solution. The presence of biosolids also significantly altered the bioavailability of Cd, Cu, Ni and Zn, as measured by soil solution, CE and free ion activity. However, this change had little effect on plant metal uptake. The length of time following treatment application had the greatest effect on soil chemistry and metal availability, resulting in pH decreases and increases in DOC, soil solution salinity, Ca and Mg. The free ion activities of each metal increased with time, as did soil solution Cd and Zn and CE-Cu, with results for Zn indicative of migration through the soil profile with time. Plant uptake of Ni and Zn also changed with time. Nickel concentrations in shoots decreased, while concentrations of Zn in shoots increased. The findings from the two studies demonstrated that biosolids increased the amount of DOC, salinity, Ca and Mg present in soil solution. In the lysimeter study measures of metal availability were affected in soils amended with biosolids, but this did not effect shoot concentrations. The overall predictive strengths of the six potential measures of bioavailability was investigated using results from the previously described experiments and related studies carried out by ESR and Lincoln University using nine different soils amended with combinations of biosolids and metal salts. Of the four metals Ni provided the strongest correlations between metal bioavailability and shoot concentrations, with 0.05 M Ca(NO₃)₂ extraction giving the strongest relationship for Ni concentrations in shoots (r² = 0.73). This suggests that the solubility of Ni is highly indicative of shoot concentrations and that Ca(NO₃)₂ is a robust measure of Ni bioavailability. In addition Ca(NO₃)₂ provided the best estimate of Zn bioavailability (r² = 0.65), and CE-Cd provided the best measure of Cd bioavailability, although it could only describe 47 % of shoot Cd concentration. Results for Cu were typical of previously described studies as assays of Cu availability are almost always poorly correlated with shoot concentrations, with total Cu having the strongest relationship (r² = 0.34). Methods based on the extractability and solubility of Cu in soils were poor indicators of Cu concentration in shoots. Overall, the addition of biosolids did not alter the outcome of these bioavailability assays, and results indicated that total metal concentrations present in the soils and biosolids matrix, plus length of time since soil treatment, had a greater affect on metal bioavailability.

Made available in DSpace on 2014-07-29T15:13:38Z (GMT). No. of bitstreams: 1 Tese_Luciano Muniz.pdf: 1589101 bytes, checksum: 4ed47c785cea4fb76d874ca98dbd4467 (MD5) Previous issue date: 2010-07-30 / When approaching the crop-livestock integration (CLI) theme, the present thesis had as one of its main objectives to evaluate the alterations in the soil-plantanimal- environment components exercised by the crop and pasture rotation dynamics in crop and livestock integration system. Two adjacent areas, one representing the natural environment (original cerrado vegetation) and other the degraded pastures served as reference ( control ) to the inferences performed. The experiment was performed at a 92 ha area, divided into six paddocks of approximately 15 ha, where the annual crops and pastures rotation was made. The soil samplings were collected at the 0-10, 10-20, 20-30, 30-40 and 40-50 cm layers. There was an increase in the carbon contents of the microbial biomass, in the microbial quotient and in the organic matter on the soil and reduction in the metabolic quotient at the pastures chronological sequence environments, at the crop-livestock integration system, in comparison with the degraded pasture. The different pastures stages within the crop-livestock integration system stocked the average of 17,67 Mg ha-1 carbon more than the degraded pasture up to a 50 cm depth. The Cerrado contained the most carbon stock and the degraded pasture the least among all the environments studied. There was a significant decrease in the phosphor and potassium contents in relation to the pasture age increase and an increase in the calcium and magnesium contents. Micronutrients contents among the treatments evaluated presented values above the demand for capim- Marandu. The capim-Marandu samplings were performed in five different seasons of the year (winter, beginning and end of spring, summer and autumn). Quantitative and qualitative alterations at the forage were observed during the experimental period. Changes on the structural components green leaf, green stem and dead material, as well as on DM (Dry Matter), GP (Gross Protein), TDN (Total Digestive Nutrients) and FND (Fiber in Neutral Detergent) contents, among others, characterized the forage on the different periods. The qualitative variations among the forages are of high magnitude, being outlined the significant drop on GP (Gross Protein) and TDN (Total Digestive Nutrients) contents according to the pasture maturity development. All pastures chronological sequence treatments, after 2,5 years of crop, resulted in favorable evaluations, having P1 treatment as the best investment option with NPV (Net Present Value) equals to R$144,34 followed by P2 treatment with R$112,61 and P3 with R$58,54, indicating to be a favorable Project. There is indication on the present work that the biggest loss in relation to the obtained economic advantages happens already from the second year after the pastures establishment. Such results may constitute important implications at CLI systems planning. Carbon bonus at pasture based animal production system may be a financial incentive for the producer to join the pastures rotation with annual crops. Nevertheless it is necessary that studies are performed to quantify emissions of gases responsible for greenhouse in order to verify whether the result represents a bonus or an environmental onus to the producer. / A presente tese, ao abordar o tema integração lavoura-pecuária (ILP), teve como um de seus principais objetivos avaliar as alterações nos componente solo-planta-animal-ambiente exercidas pela dinâmica de rotação de culturas e pastagens em sistema de integração lavoura e pecuária. Duas áreas adjacentes, uma representativa do meio ambiente natural (vegetação de cerrado original ) e outra de pastagens degradadas, serviram de referência ( controle ) às inferências realizadas. O experimento foi realizado numa área de 92 ha, dividida em seis piquetes de aproximadamente 15 ha, onde foi feita a rotação de culturas anuais e pastagens. As amostras de solo foram coletadas nas camadas 0-10, 10-20, 20-30, 30-40 e 40-50 cm. Houve aumento nos teores de carbono da biomassa microbiana, no quociente microbiano e na matéria orgânica no solo e redução no quociente metabólico nos ambientes da cronossequência das pastagens, no sistema integração lavoura-pecuária, em comparação com a pastagem degradada. As diferentes fases das pastagens dentro do sistema de integração lavoura-pecuária estocaram, em média, 17,67 Mg ha-1 mais carbono que a pastagem degradada até a profundidade de 50 cm. O Cerrado conteve o maior, e a pastagem degradada o menor estoque de carbono entre todos os ambientes estudados. Houve um decréscimo significativo nos teores de fósforo e potássio com o aumento da idade da pastagem e aumento nos teores de cálcio e magnésio. Os teores dos micronutrientes entre os tratamentos avaliados apresentaram valores acima da exigência para o capim-Marandu. As amostras de capim-Marandu foram realizadas em cinco épocas do ano (inverno, início e final da primavera, verão e outono). Alterações quantitativas e qualitativas na forragem foram observadas durante o período experimental. Mudanças nos componentes estruturais folha verde, colmo verde e material morto, bem como nos teores de matéria seca (MS), proteína bruta (PB), nutrientes disponíveis totais (NDT) e fibra em detergente neutro (FDN), entre outros, caracterizaram a forragem nos diferentes períodos. As variações qualitativas entre as forrageiras são de alta magnitude, destacando-se a queda significativa nos teores de PB e NDT, com o avanço da maturidade da pastagem. Todos os tratamentos da cronossequência das pastagens, após 2,5 anos de lavoura, resultaram em avaliações favoráveis, sendo o tratamento P1 a melhor opção de investimento, com valor presente líquido (VPL) igual a R$ 144,34, seguido pelo tratamento P2 com R$ 112,61 e P3 com R$ 58,54, indicando tratar-se de projeto favorável. Há indicação no presente trabalho que a maior perda em relação às vantagens econômicas obtidas ocorre já a partir do segundo ano após o estabelecimento das pastagens, podendo constituir implicações importantes no planejamento de sistemas de ILP. O bônus de carbono no sistema de produção animal a pasto pode ser um incentivo financeiro para o produtor aderir à rotação de pastagens com culturas anuais. Porém é preciso que sejam realizados estudos que quantifiquem as emissões de gases responsáveis pelo efeito estufa, para verificar se o balanço representa um bônus ou um ônus ambiental para o produtor.

A two-year lysimeter study was undertaken to compare the environmental effects (e.g. nitrate leaching and nitrous oxide emissions) of soil applied lactose-depleted dairy factory effluent (LD-DFE) with lactose-rich DFE. The aim of this experiment was to determine the fate of nitrogen from LD-DFE and dairy cow urine applied to a Templeton fine sandy loam soil (Udic Ustrochrept), supporting a herbage cover of ryegrass (Lolium perenne) and white clover (Trifolium repens). Measurements were carried out on the amount of nitrogen lost from the soil via leaching, lost by denitrification, removed by the pasture plants, and immobilized within the soil organic fraction. Further, a comparison between the fate of nitrogen in LD-DFE irrigated onto land under a "cut and carry" system, as opposed to a "grazed" pasture system was undertaken. Lactose-depleted dairy factory effluent was applied at three-weekly intervals during the summer months at rates of 25 and 50 mm, until nitrogen loading targets of 300 and 600 kg N ha⁻¹ yr⁻¹ had been achieved. Measured leaching losses of nitrogen averaged 2 and 7 kg N ha⁻¹ yr⁻¹ for Control 25 and Control 50 treatments; 21, 20 and 58 kg N ha⁻¹ yr⁻¹ for 25 and 50 mm "cut and carry" treatments respectively; and 96 kg N ha⁻¹ yr⁻¹ for the 25 mm "grazed" treatment. The range of nitrate-N leaching loss from LD-DFE plus urine is no different from the lactose-rich DFE nitrate leaching loss. Uptake of nitrogen by the growing pasture averaged 153, 184,340,352,483, and 415 kg N ha⁻¹ yr⁻¹ for Control 25, Control 50, LD-DFE 25 and LD-DFE 50 mm "cut and carry" treatments, and the LD-DFE 25 mm "grazed" treatment, respectively. Denitrification losses were 0.06, 4.4, 1.69, 19.70, and 7.4 kg N ha⁻¹ yr⁻¹ for Control 25, the LD-DFE 25 "cut and carry" treatments, the LD-DFE 25 mm "grazed" treatment, and calculated "paddock losses", respectively. Isotopic nitrogen studies found that 29.4 and 25.8% of applied LD-DFE nitrogen was immobilised in the LD-DFE 25 and LD-DFE 50 "cut and carry" treatments. The results of this experiment confirm the findings of the previous lactose-rich DFE study, in that the effects of grazing stock are of greater environmental concern than the removal of lactose from the effluent waste stream.