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Efeito do feijão caupi (Vigna unguiculata L. Walp) e da proteína isolada no metabolismo lipídico em hamsters hipercolesterolemizados / Effect of the whole seed and protein isolate of cowpea (Vigna unguiculata L. Walp) on the lipid metabolism of hypercholesterolemic hamstersFrota, Karoline de Macedo Gonçalves 05 June 2007 (has links)
Introdução - A soja e outras leguminosas são consideradas como alimentos funcionais por apresentarem propriedades hipocolesterlemizantes. Esta propriedade, porém, ainda não foi relatada para o feijão caupi. Um possível componente deste grão responsável pelo feito redutor de colesterol é sua proteína. Objetivo - Produzir isolado protéico de feijão caupi e verificar a influência do grão integral e de sua proteína isolada no metabolismo lipídico de hamsters hipercolesterolemizados pela dieta. Métodos - O isolado protéico de feijão caupi foi produzido por precipitação isoelétrica, utilizando-se pH 8,5 para solubilização da proteína e 4,5 para a sua precipitação, obtendo-se um isolado com 92 % de proteína. O isolado protéico (IP) e o feijão caupi integral (FCI) foram utilizados como fonte protéica em dietas experimentais para hamsters que tiveram hipercolesterolemia induzida por dieta contendo 20 % de caseína, 13,5 % de gordura saturada e 0,1 % de colesterol, por 3 semanas. Os animais foram distribuídos em três grupos, recebendo cada grupo dieta com 20 % de caseína (controle), dieta com 20 % de proteína de isolado de feijão caupi e dieta com 20% de proteína de feijão caupi integral, por 4 semanas. Resultados - Comparando-se à dieta controle, a dieta com FCI e IP provocaram reduções significativas no colesterol total (49 % e 20 %, respectivamente) e colesterol não-HDL (54 % e 22 %, respectivamente). Análises histológicas do fígado foram realizadas e observou-se que IP e o FCI proporcionaram efeito hepatoprotetor comparado à caseína, pois o grupo que recebeu caseína apresentou em média esteatose difusa e intensa, enquanto grupo com feijão e isolado apresentaram esteatose focal e, em alguns casos, ausentes. Alguns possíveis mecanismos envolvidos para o efeito benéfico no metabolismo lipídico foram investigados. A digestibilidade verdadeira do IP foi igual à da caseína, enquanto a do feijão integral foi menor. A excreção de ácidos biliares e colesterol foi inversamente proporcional aos níveis plasmáticos do colesterol dos animais submetidos às diferentes dietas. Os animais com a dieta de feijão integral apresentaram a maior excreção de ácidos biliares e colesterol nas fezes; valores estatisticamente diferentes aos dos animais da dieta com caseína. Conclusões - O feijão caupi e sua proteína isolada reduzem o colesterol plasmático e proporcionam efeito hepatoprotetor. A digestibilidade, excreção de ácidos biliares e colesterol não estão relacionados com a redução do colesterol provocada pelo IP. O mecanismo envolvido na redução do colesterol nestes experimentos ainda não está totalmente elucidado, sugerindo que a proteína isolada do feijão caupi atue na síntese de colesterol, pois não foi observado aumento na sua excreção. / Introduction - Soya and other legume seeds are considered functional food because of their hypocholesterolemic properties. This property, however, is not reported yet for cowpea. A possible component present in this grain that could respond for the hypocholesterolemic effect is the protein fraction. Objective - To produce protein isolate of cowpea and to verify the influence of the whole seed and its isolated protein on the lipid metabolism of diet hypercholesterolemized hamsters. Methods - The cowpea protein isolate was prepared by isoelectric precipitation, using pH 8.5 for solubilization and pH 4.5 for protein precipitation. The isolate obtained presented protein content of about 92%. The protein isolate (PI) and cowpea whole seed (CWS) were used as protein source in experimental diets fed to hamsters that previously had their blood cholesterol increased by a diet containing 20 % of casein, 13,5 % of saturated fat and 0,1 % of cholesterol during 3 weeks. Animals were divided into 3 groups and fed on diets containing: 20 % casein (control), 20 % isolate protein of cowpea and 20 % of cowpea whole seed for 4 wks. Results - The results showed that for hamster that fed on diets containing PI and CWS occurred a significant decrease in total cholesterol (49 % and 20 %, respectively) and non-HDL cholesterol (54 % and 22 %, respectively), as compared to casein group. Histological analysis of hepatic tissue was performed and showed that PI and CWS presented reduction in hepatic lipotoxicity as compared to the casein group. Therefore, the group that received casein presented steatosis widely distributed throughout the hepatic lobule, while whole seed cowpea and protein isolate meal groups produced only focal steatosis and, in some cases, it was absent. Some mechanisms involved in lowering plasma cholesterol were investigated. Digestibility for PI was equal for casein group, while it was significantly lower for CWS group. The bile acids and cholesterol excretion in the feces were inversely proportional to plasma cholesterol levels. Animals on CWS presented higher levels of feces bile acids and cholesterol; these values were statistically different from animals on casein diet. Conclusions - Whole seed cowpea and its protein isolate reduce plasma cholesterol and hepatic lipotoxicity. Digestibility, the bile acids and cholesterol excretion are not related to hypocholesterolemic effect of protein isolate of cowpea. The mechanisms involved cholesterol reduction in these experiments is not yet fully elucidated. It is suggested that protein isolate of cowpea is related to the cholesterol syntheses, as it was not verified any increase in cholesterol excretion in the animals on protein isolate diet.
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Efeito do feijão caupi (Vigna unguiculata L. Walp) e da proteína isolada no metabolismo lipídico em hamsters hipercolesterolemizados / Effect of the whole seed and protein isolate of cowpea (Vigna unguiculata L. Walp) on the lipid metabolism of hypercholesterolemic hamstersKaroline de Macedo Gonçalves Frota 05 June 2007 (has links)
Introdução - A soja e outras leguminosas são consideradas como alimentos funcionais por apresentarem propriedades hipocolesterlemizantes. Esta propriedade, porém, ainda não foi relatada para o feijão caupi. Um possível componente deste grão responsável pelo feito redutor de colesterol é sua proteína. Objetivo - Produzir isolado protéico de feijão caupi e verificar a influência do grão integral e de sua proteína isolada no metabolismo lipídico de hamsters hipercolesterolemizados pela dieta. Métodos - O isolado protéico de feijão caupi foi produzido por precipitação isoelétrica, utilizando-se pH 8,5 para solubilização da proteína e 4,5 para a sua precipitação, obtendo-se um isolado com 92 % de proteína. O isolado protéico (IP) e o feijão caupi integral (FCI) foram utilizados como fonte protéica em dietas experimentais para hamsters que tiveram hipercolesterolemia induzida por dieta contendo 20 % de caseína, 13,5 % de gordura saturada e 0,1 % de colesterol, por 3 semanas. Os animais foram distribuídos em três grupos, recebendo cada grupo dieta com 20 % de caseína (controle), dieta com 20 % de proteína de isolado de feijão caupi e dieta com 20% de proteína de feijão caupi integral, por 4 semanas. Resultados - Comparando-se à dieta controle, a dieta com FCI e IP provocaram reduções significativas no colesterol total (49 % e 20 %, respectivamente) e colesterol não-HDL (54 % e 22 %, respectivamente). Análises histológicas do fígado foram realizadas e observou-se que IP e o FCI proporcionaram efeito hepatoprotetor comparado à caseína, pois o grupo que recebeu caseína apresentou em média esteatose difusa e intensa, enquanto grupo com feijão e isolado apresentaram esteatose focal e, em alguns casos, ausentes. Alguns possíveis mecanismos envolvidos para o efeito benéfico no metabolismo lipídico foram investigados. A digestibilidade verdadeira do IP foi igual à da caseína, enquanto a do feijão integral foi menor. A excreção de ácidos biliares e colesterol foi inversamente proporcional aos níveis plasmáticos do colesterol dos animais submetidos às diferentes dietas. Os animais com a dieta de feijão integral apresentaram a maior excreção de ácidos biliares e colesterol nas fezes; valores estatisticamente diferentes aos dos animais da dieta com caseína. Conclusões - O feijão caupi e sua proteína isolada reduzem o colesterol plasmático e proporcionam efeito hepatoprotetor. A digestibilidade, excreção de ácidos biliares e colesterol não estão relacionados com a redução do colesterol provocada pelo IP. O mecanismo envolvido na redução do colesterol nestes experimentos ainda não está totalmente elucidado, sugerindo que a proteína isolada do feijão caupi atue na síntese de colesterol, pois não foi observado aumento na sua excreção. / Introduction - Soya and other legume seeds are considered functional food because of their hypocholesterolemic properties. This property, however, is not reported yet for cowpea. A possible component present in this grain that could respond for the hypocholesterolemic effect is the protein fraction. Objective - To produce protein isolate of cowpea and to verify the influence of the whole seed and its isolated protein on the lipid metabolism of diet hypercholesterolemized hamsters. Methods - The cowpea protein isolate was prepared by isoelectric precipitation, using pH 8.5 for solubilization and pH 4.5 for protein precipitation. The isolate obtained presented protein content of about 92%. The protein isolate (PI) and cowpea whole seed (CWS) were used as protein source in experimental diets fed to hamsters that previously had their blood cholesterol increased by a diet containing 20 % of casein, 13,5 % of saturated fat and 0,1 % of cholesterol during 3 weeks. Animals were divided into 3 groups and fed on diets containing: 20 % casein (control), 20 % isolate protein of cowpea and 20 % of cowpea whole seed for 4 wks. Results - The results showed that for hamster that fed on diets containing PI and CWS occurred a significant decrease in total cholesterol (49 % and 20 %, respectively) and non-HDL cholesterol (54 % and 22 %, respectively), as compared to casein group. Histological analysis of hepatic tissue was performed and showed that PI and CWS presented reduction in hepatic lipotoxicity as compared to the casein group. Therefore, the group that received casein presented steatosis widely distributed throughout the hepatic lobule, while whole seed cowpea and protein isolate meal groups produced only focal steatosis and, in some cases, it was absent. Some mechanisms involved in lowering plasma cholesterol were investigated. Digestibility for PI was equal for casein group, while it was significantly lower for CWS group. The bile acids and cholesterol excretion in the feces were inversely proportional to plasma cholesterol levels. Animals on CWS presented higher levels of feces bile acids and cholesterol; these values were statistically different from animals on casein diet. Conclusions - Whole seed cowpea and its protein isolate reduce plasma cholesterol and hepatic lipotoxicity. Digestibility, the bile acids and cholesterol excretion are not related to hypocholesterolemic effect of protein isolate of cowpea. The mechanisms involved cholesterol reduction in these experiments is not yet fully elucidated. It is suggested that protein isolate of cowpea is related to the cholesterol syntheses, as it was not verified any increase in cholesterol excretion in the animals on protein isolate diet.
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Influence of SO2 fumigation on growth, photosynthesis, lipoxygenase and peroxidase activities of soybean (Glycine max), in open-top chambers / Susan LindequeLindeque, Susan January 2012 (has links)
Air pollutant exposure poses a health risk to humans and impacts negatively on agriculture. High levels of air pollution resulted in extensive crop damage and yield reduction in Europe and USA. The Highveld region in South Africa, a very important area for maize and soya production, has already been declared an air pollution hot spot, with SO2 being the most concerning air pollutant. Most of the SO2 over the Highveld originates from the burning of coal for power generation. Developing countries, such as South Africa, are highly dependent on agriculture for food security and high levels of air pollution pose serious risks to the agricultural industry. Currently very little information is available on the effects of air pollution on crop production in South Africa.
This study aimed to establish exposure-response relationship for SO2 on soybean and the quantification thereof on the morphological, physiological and biochemical characteristics. Two soybean cultivars were used, namely: LS 6164 and PAN 1666. The plants were fumigated for 7 hours, 7 days a week with 0 (carbon filtered control; CF), 25, 75 and 150 ppb SO2. The effect of SO2 was investigated on the growth, photosynthetic capabilities, photosynthetic gas exchange, peroxidase activity and lipoxygenase activity of the cultivars.
Foliar injuries and interveinal chlorosis were visible with increasing levels of SO2 as well as a decrease in biomass accumulation, especially in root biomass; a more prominent feature of LS 6164. The number of nodules of both cultivars decreased insignificantly as the levels of SO2 increased. The number of pods per plant and the average weight of 30 seeds indicated a downward trend with an increase in SO2 concentration. The chlorophyll content of PAN 1666 was lower compared to LS 6164. PAN 1666 had the largest reduction in stomatal conductance at 150 ppb SO2 fumigation.
The photosynthetic vitality index indicated that LS 6164 was more sensitive to SO2 inhibition from 25 ppb SO2 and higher, whereas PAN 1666 mostly became sensitive to SO2 from 75 ppb SO2. A decrease in the ability to absorb light energy, the trapping of excitation energy to transfer electrons beyond QA-, and the reduction of end electron acceptors all contributed to the decline in the vitality index.
Sulphur content increased significantly in the 75 ppb and 150 ppb treatments of both cultivars. Induced peroxidase and lipoxygenase activity was seen in both cultivars, especially at higher concentrations of SO2 treatments. PAN 1666 had a higher rate of peroxidase and lipoxygenase activity compared to LS 6164.
The implication for SO2 on crop production in the highly industrial Highveld area was demonstrated to be potentially of great concern. The dose-response relationships plotted for OJIP parameters emphasized that SO2 is an inhibitor of photosynthesis and phytotoxic of nature. Both cultivars experienced limitations from 75 ppb, especially at the 150 ppb SO2 concentration. From these results it appears that PAN 1666 is more adapted to SO2 compared to LS 6164 and levels of 75 ppb SO2 and higher become toxic to these plants. / Thesis (Master of Environmental Sciences)--North-West University, Potchefstroom Campus, 2013
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Influence of SO2 fumigation on growth, photosynthesis, lipoxygenase and peroxidase activities of soybean (Glycine max), in open-top chambers / Susan LindequeLindeque, Susan January 2012 (has links)
Air pollutant exposure poses a health risk to humans and impacts negatively on agriculture. High levels of air pollution resulted in extensive crop damage and yield reduction in Europe and USA. The Highveld region in South Africa, a very important area for maize and soya production, has already been declared an air pollution hot spot, with SO2 being the most concerning air pollutant. Most of the SO2 over the Highveld originates from the burning of coal for power generation. Developing countries, such as South Africa, are highly dependent on agriculture for food security and high levels of air pollution pose serious risks to the agricultural industry. Currently very little information is available on the effects of air pollution on crop production in South Africa.
This study aimed to establish exposure-response relationship for SO2 on soybean and the quantification thereof on the morphological, physiological and biochemical characteristics. Two soybean cultivars were used, namely: LS 6164 and PAN 1666. The plants were fumigated for 7 hours, 7 days a week with 0 (carbon filtered control; CF), 25, 75 and 150 ppb SO2. The effect of SO2 was investigated on the growth, photosynthetic capabilities, photosynthetic gas exchange, peroxidase activity and lipoxygenase activity of the cultivars.
Foliar injuries and interveinal chlorosis were visible with increasing levels of SO2 as well as a decrease in biomass accumulation, especially in root biomass; a more prominent feature of LS 6164. The number of nodules of both cultivars decreased insignificantly as the levels of SO2 increased. The number of pods per plant and the average weight of 30 seeds indicated a downward trend with an increase in SO2 concentration. The chlorophyll content of PAN 1666 was lower compared to LS 6164. PAN 1666 had the largest reduction in stomatal conductance at 150 ppb SO2 fumigation.
The photosynthetic vitality index indicated that LS 6164 was more sensitive to SO2 inhibition from 25 ppb SO2 and higher, whereas PAN 1666 mostly became sensitive to SO2 from 75 ppb SO2. A decrease in the ability to absorb light energy, the trapping of excitation energy to transfer electrons beyond QA-, and the reduction of end electron acceptors all contributed to the decline in the vitality index.
Sulphur content increased significantly in the 75 ppb and 150 ppb treatments of both cultivars. Induced peroxidase and lipoxygenase activity was seen in both cultivars, especially at higher concentrations of SO2 treatments. PAN 1666 had a higher rate of peroxidase and lipoxygenase activity compared to LS 6164.
The implication for SO2 on crop production in the highly industrial Highveld area was demonstrated to be potentially of great concern. The dose-response relationships plotted for OJIP parameters emphasized that SO2 is an inhibitor of photosynthesis and phytotoxic of nature. Both cultivars experienced limitations from 75 ppb, especially at the 150 ppb SO2 concentration. From these results it appears that PAN 1666 is more adapted to SO2 compared to LS 6164 and levels of 75 ppb SO2 and higher become toxic to these plants. / Thesis (Master of Environmental Sciences)--North-West University, Potchefstroom Campus, 2013
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The development of Amadumbe (Colocasia esculenta (l.) schott)-soya composite biscuits with improved nutritional and sensory propertiesMokhele, Tabea Mokgalakane 06 1900 (has links)
The Amadumbe crop [Colocasia esculenta (L.) Schott] is a traditional Southern African tuber
crop which is rich in starch, mucilage and micronutrients. Amadumbe tubers have limited
amount of proteins and as a result, amadumbe-processed foods lack adequate protein. The
purpose of this study was therefore to develop protein-rich amadumbe-soya composite biscuits,
which would be acceptable to consumers. Biscuits were prepared by combining amadumbe and
soya flours at ratios: 90:10, 70:30 and 50:50. Functional properties of composite flours and the
physical properties of composite biscuits were determined. The proximate composition, amino
acid composition and protein digestibility of composite biscuits were determined. Consumer
acceptability test of biscuits was performed using nine-point hedonic scale. The results
indicated that the 90% amadumbe and 10% soya composite biscuits had high significant values
of moisture, ash, carbohydrates contents and energy values. The 50% amadumbe and 50% soya
composite biscuits had significantly high values of fat, crude protein contents and acid
detergent fibre (ADF). The protein digestibility, amino acid contents, especially the lysine
contents of composite biscuits increased significantly (p ≤ 0.05) with an increase in the
percentage of soya. The mineral contents of composite biscuits; Ca, Mg, P, Zn, Cu, Mn and Fe
increased significantly (p ≤ 0.05) with the increase of soya in the composite biscuits. There was
a significant difference in the mean taste acceptability and mean overall acceptability when the
soya concentration was increased to 50%. Soya was successfully used to produce amadumbe
composite biscuits with better nutritional quality with respect to protein content, amino acid
profile and selected mineral contents and which were acceptable to consumers. / National Research Foundation (South Africa) / Life and Consumer Science / M.Sc. (Agriculture)
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Inserção competitiva do Complexo de Soja Brasileiro no mercado mundial: os efeitos da taxa de câmbio real sobre os preços desses produtos. / Competitive insertion of the Brazilian Soybean Complex in the world market: the effects of the real exchange rate on the prices of these products.MACEDO, Luziene Dantas de. 12 September 2018 (has links)
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Previous issue date: 2003 / O presente trabalho tem como objetivo analisar a inserção competitiva do complexo soja
brasileiro no mercado mundial e os efeitos da taxa de câmbio real brasileira sobre os preços desses produtos. Através de um método estatístico de decomposição dos preços dos produtos em moeda nacional constatou-se que o Brasil, ao longo do período em estudo, tem buscado uma verdadeira fonte empreendedora para a busca de receitas de exportação, através do desenvolvimento da cadeia agro-industrial da soja. Outrossim, o ambiente internacional competitivo no qual essa cadeia se insere, torna o país um tomador de preços desses produtos no mercado mundial e apresenta desafios que envolvem desde uma adequação aos padrões concorrenciais vigentes na economia mundial, até a busca de uma maior valorização do produto em termos de aumento de valor agregado, no sentido de aumentar a sua competitividade no cenário internacional. / The present work has as aim to analyse the competitive insertion of the brazilian soybean
complex in world market and the effects of brazilian real exchange rate on the prices of
these products. Employing a statistical method of decomposition of these prices in national
currency, one perceived that Brazil, during ali the period under study, is searching for a
trully enterprising source to look for export receipts, through the development of the
soybean agro-industrial chain. Concomitantly, the competitive intemational environment in
which this chain is inserted makes the country a price taker in these products in world
market and presents challenges that involves since an arrangement to the existing
competitive patterns in world economy, till the search for a greater valorization of the
product in aggregate value, to increase the competitiveness in intemational scene.
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Evolución de la producción y comercialización de semillas de soja en Paraguay (1997/2013) / Changes in the production and marketing of soya seeds in Paraguay (1997/2013)Gamarra, Maria Estela Ojeda 06 June 2014 (has links)
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Previous issue date: 2014-06-06 / Sem bolsa / La producción de soja en Paraguay junto con los demás productos del sector agrícola, como la ganadería y la producción forestal han llevado a generar el mayor crecimiento de la economía en los últimos tiempos, siendo las previsiones de crecimiento para el 2013 de 13%. Si bien la producción de soja se inicia en la década de 1970 como complemento del programa de trigo, no es hasta finales de 1.990 que se produce una gran expansión del área de producción con la adopción de la siembra directa y la soja RR. Este trabajo tiene por objetivo hacer una revisión de la información existente sobre la producción de soja y su relación con la comercialización de semilla en el periodo de 1997 a 2013. En el 2004 se aprueba el uso comercial por primera vez de variedades de soja transgénica, soja RR, pero anteriormente ya se reporta datos de introducción de soja RR sin autorización legal, razón por la cual no se cuenta con datos de las primeras introducciones al sistema productivo, estos se tienen a partir recién del 2005. La línea de tendencia de la comercialización de semillas nos muestra una tendencia alcista, pero aún no satisface las necesidades de semillas. La ley 385/94 de Semillas y Protección de Cultivares y la aprobación comercial del evento de soja RR, junto con el acuerdo de toda la cadena de producción de reconocer los derechos de propiedad intelectual de la tecnología de soja RR, fueron incentivos para que empresas dueñas de germoplasmas se establecieran en el país y fueran las responsables de la introducción de variedades cada vez mejor adaptadas a las condiciones climáticas locales y a la tecnología de producción de los agricultores. En cuanto a la industria semillera nacional, la misma lentamente va aumentado su participación en el comercio de semillas e invirtiendo en tecnologías que permiten mejorar la calidad de las semillas ofertadas al mercado. / Soya production in Paraguay, together with other products coming from the agricultural sector such....
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Aproveitamento de sementes de soja após o beneficiamento / Efficiency in soybean seed production process, considering the quality control at post-harvestingVedan, Amanda Cabral Calusci 10 September 2015 (has links)
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Previous issue date: 2015-09-10 / Sem bolsa / A soja, Glycine max, é a menina dos olhos da exportação no Brasil, sendo no processo produtivo, a semente tem papel de destaque, pois além de ser o promotor do estabelecimento da nova lavoura, leva consigo um pacote tecnológico de alta eficiência e baixo custo que, em função da sua compreensão em si e, pela inserção de genes específicos, abrem campo à diferenciação de praticas agronômica. Neste contesto, sementeiras necessitam reavaliar o processo produtivo tornando-o mais eficiente, com maior aproveitamento. Por isso o presente trabalho é um estudo de caso de três safras, comparando a eficiência do controle de qualidade pós-colheira, no processo de produção de semente. Tendo como objetivo determinar a eficiência nos processo de produção, buscando determinar o aproveitamento da semente referente a qualidade pós-colheita. O estudo de caso fói desenvolvido no Laboratório de Analise Sementes da Nidera Sementes, Uberlândia – MG. Foram avaliados três safras, 2011/2012, 2012/2013, 2013/2014, todas as cultivares produzidas em cada uma destas, sendo que a Cultivar que se repetiu em todas foi a NA 5909 RG, sendo que sobre esta pode-se comprar seu aproveitamento por qualidade em pós-colheita em cada safra.Considerou-se a média por cultivar do total beneficiado e a média do total aprovado. Também observou em sacos de 40 kg a média do descarte e fez-se a porcentagem de aproveitamento por cultivar. Conclui-se que a eficiência na produção de semente de soja difere acentuadamente entre cultivares e entre safras e que o processo tem que ser reavaliado, para que esses números de descartes sejam baixados, pois os custos agregados são elevados, necessitando de um melhor planejamento. / Soybean, Glycine max, is the apple of the eyes of exports in Brazil, where seeds have an important role to promote the establishment of a new crop. Seed must have quality and be produced in an efficient way in order to have an adequate price and availability to the farmer.This paper is a case study of three harvesting season comparing the efficiency in quality control at post harvesting. The case study was conducted at the seed analysis laboratory of a seed company of central part of Brazil. The crop years were, 2011/2012, 2012/2013, 2013/2014 and in all years cultivars were compared as the range of the germination test. It was concluded that the efficiency in soybean seed production differs markedly among cultivars and crop years where the process has to be reevaluated, considering the high discharge rate due to low germination after seed processing.
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Développement d'outils de sélection génomique assistée par marqueurs pour la lutte au nématode à kyste du sojaBoucher-St-Amour, Vincent-Thomas 06 April 2024 (has links)
La culture du soja (Glycine max (L.) Merr.) a connu une forte expansion au Canada surtout grâce au développement de lignées à maturité hâtive facilitant sa culture plus au nord. En parallèle le nématode à kyste du soja (NKS ; Heterodera glycines Ichinohe), parasite du soja, est devenu le premier ravageur du soja mondialement et il s’installe progressivement au Canada. L’utilisation de cultivars résistants demeure le moyen le plus efficace pour réduire les pertes associées au NKS. Cependant une seule source de résistance a été largement exploitée en Amérique, soit l’accession PI 88788. Son utilisation intensive a exercé une forte pression de sélection sur le NKS pouvant maintenant surmonter cette résistance presque partout aux États-Unis. De plus PI 88788 présente une maturité très tardive la rendant mal adaptée aux régions canadiennes. Cette étude visait à déterminer les régions génomiques conférant la résistance au NKS chez la variété de soja Suzuhime (PI 494182) laquelle affiche une grande résistance au NKS et la maturité la plus hâtive parmi les accessions résistantes. Pour ce faire 149 lignées issues d’un croisement entre la variété Costaud (haut rendement, maturité hâtive, mais sensible au NKS) et Suzuhime ont été génotypées et évaluées pour leur résistance au NKS permettant de réaliser une analyse QTL (« quantitive trait loci ») et ainsi identifier les locus de résistance. Ces lignées ont aussi été évaluées pour certains caractères agronomiques dans le but de déterminer l’impact de la sélection des allèles de résistance sur les performances agronomiques. Finalement, grâce au reséquençage de PI 494182 et Costaud, nous avons identifié les allèles en présence pour certains gènes de résistance connus. Ce travail permettra d’identifier des marqueurs génétiques de résistance au NKS et ainsi faciliter le développement de cultivars combinant maturité hâtive, rendement élevé et résistance au NKS.
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Interaction entre le nématode à kyste (Heterodera glycines) et Phytophthora sojae chez le soyaAudette, Carolane 27 March 2024 (has links)
Le nématode à kyste du soya (NKS), Heterodera glycines, est la principale cause de pertes économiques chez le soya aux États-Unis. Au Canada, on le retrouve en Ontario depuis 1988 et il continue à gagner du terrain chaque année. Il a été répertorié pour la première fois au Québec en 2013, et les producteurs de soya québécois ont déjà commencé à déployer des outils pour sa répression. La méthode de lutte la plus utilisée contre cet organisme est l’utilisation de gènes de résistance quantitative combiné à la rotation des cultures. Par contre, l’organisme causant le plus de dommages chez le soya à l’heure actuelle au Québec est la pourriture phytophthoréenne, causée par l’oomycète, Phytophthora sojae. Cette maladie racinaire peut, elle aussi, être réprimée grâce à des cultivars génétiquement résistants (résistance qualitative à gènes Rps). Sachant que le NKS se retrouve déjà dans certaines régions du Québec où sévit également P. sojae, il devient urgent de déterminer si les cultivars de soya présentement utilisés exprimeront une résistance satisfaisante contre ces agents pathogènes majeurs. Dans ce contexte, l’objectif principal de ce projet était d’évaluer l’interaction entre ces deux agents pathogènes du soya en fonction de la résistance (qualitative à gènes Rps / résistance quantitative au NKS) des cultivars utilisés. L’étude, réalisée en système hydroponique, a permis de démontrer qu’en présence d’un cultivar sensible aux deux organismes, P. sojae a un impact négatif sur la capacité du NKS à produire des kystes. Cela suggère que P. sojae pourrait influencer le développement du NKS. La présence du nématode, quant à elle, n’a pas influencé l’efficacité de la résistance qualitative à gènes Rps contre P. sojae. En conséquence, les cultivars résistants aux deux agents pathogènes représentent une bonne option pour prévenir et lutter contre ces affections. / The soybean cyst nematode (SCN), Heterodera glycines, is the leading cause of economic losses in soybean in the United States. In Canada, it has been found in Ontario in 1988 and continues to spread every year. It was identified for the first time in Quebec in 2013 and soybean growers have already begun to deploy management tools. The most commonly used control method against SCN is the use of quantitative resistance genes and crop rotation. However, the most damaging organism in soybean in Quebec currently is Phytophthora root rot, caused by Phytophthora sojae. This root disease can also be controlled by genetically resistant cultivars (qualitative resistance with Rps genes). Because SCN is now present in regions struggling with P. sojae, it is important to determine if the cultivars will express sufficient resistance against these two major pathogens. In this context, the main objective of this project was to evaluate the interaction between these two organisms on soybean, according to the resistance (qualitative Rps genes/ or quantitative resistance to SCN) of the cultivars used. This study, in a hydroponic system, demonstrated that P. sojae has a negative impact on SCN capacity to produce cysts produced. This suggests that P. sojae might influence SCN development. On the other hand, resistant cultivars were not affected by the presence of both pathogens and the presence of the nematode did not influence the efficacy of qualitative Rps resistance gene against P. sojae. As a result, cultivars resistant to both pathogens are a good option for preventing and controlling these diseases.
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