Sweet Tooth is an exploration of childhood culture as it exists in an adult world. I am interested in the power dynamics resulting from the cohabitation of youth and adults, and the manner in which adults impose their knowledge, faith, and morals upon children. Through the watercolour paintings and textile sculptures in Sweet Tooth, I investigate nostalgia, childhood playthings and childrens literature, especially cautionary tales and religious texts for children. These morality tales are a product of Victorian-era theories of youth education and child rearing. While much has changed in the past 100 years, the impact of this era can still be felt, especially in conservative religious cultures such as that which informed my own youth. The childrens stories produced during this particular time use a mixture of scare-tactics and theological themes to convey their message. In Victorian times, there was an apprehension about failing the intellectual, physical, and spiritual needs of children. This was compensated for with well-intentioned, but peculiar, attempts to frighten youngsters into strict obedience. The painting style in my exhibition, and my use of the watercolour medium, recalls the colourful imagery found in Victorian-era books for children. The textiles in my sculptural works relate to treasured childhood toys. My paintings and sculptures reference the human bodies of both children and adults and employ dramatic shifts in scale. Conceptually, the artwork challenges didactic lessons, but, nevertheless, the children in the paintings appear to crave approval from authority figures. The illustrations of bodies in Sweet Tooth defiantly reveal their imperfections and limitations, but also display a playful humour and desire for worldly delights. The conceptual themes of my art stem from sentimental and romantic views of childhood and my desire is to dissect and expose the actual struggles children endured in past generations, and continue to experience today. These themes recall the Christian teachings during my formative years and relate to adult recollections of youthful guilt and punishment.

This thesis explores the oppression of women within the gender institution of patriarchy in Margaret Atwood¡¦s dystopian novel The Handmaid¡¦s Tale, and their resistance to this male-dominated society. As a feminist writer, Atwood is very much concerned about the issue of gender, which she foregrounds in The Handmaid¡¦s Tale. In my analysis, I apply some theories of radical feminists and the French feminist who devote themselves to the study of gender--Kate Millett, Adrienne Rich, Catherine MacKinnon, and Hélène Cixous. Millett focuses on women¡¦s subordinated position that leads to women¡¦s oppression in patriarchy. Rich and MacKinnon focus on how women are controlled and oppressed in maternity and sexuality within the patriarchal society of gender inequality. Cixous challenges the validity of gender by pointing out its characteristic fluidity through creating woman¡¦s own writing in order to redefine female selfhood for women¡¦s resistance. The thesis is composed of five chapters. The Introduction presents the background materials about Atwood and The Handmaid¡¦s Tale, the motivation of the thesis, and the resonance between The Handmaid¡¦s Tale and certain feminists¡¦ theories. The first chapter analyzes the formation of the unbalanced power relations between the sexes in which women are subordinated to men through the socialization. Moreover, because of women¡¦s subordination, women are modulated as mothers through socially institutionalized motherhood such as the Wives and the Handmaids in Gilead. The second chapter further analyzes how women are formulated as sexual objects through the experience of sexual objectification within the institution of heterosexuality, such as the mistresses and the prostitutes of Gilead. The third chapter discusses how female orality empowers women to resist their patriarchal society in The Handmaid¡¦s Tale. The protagonist Offred, by ¡§writing her voice¡¨ through storytelling, resists patriarchal oppression, restores her body and self, and transforms herself from a victim in a claustrophobic world of male domination to a heroine of femininity. Moreover, her act of writing by her voice also reflects women¡¦s histories of repression, which should be reconstructed in a culture in which only males are literate. Offred¡¦s oral act of storytelling, to the reader, may also signify her resistance to reconstruct women¡¦s repressed histories. The concluding chapter reiterates the research of The Handmaid¡¦s Tale with a synthesis of Atwood¡¦s and some of the prominent feminists¡¦ points of view, namely Millett¡¦s, Rich¡¦s, MacKinnon¡¦s and Cixous¡¦s, toward the oppression and resistance of women within the institution of gender. This study hopes to explore and thus illuminate the nature, the functioning, the operation of socially constructed male domination, and then proceed to search the possible solution, or the ¡§voice;¡¨ however feeble it is, the author, or the protagonist conceives to defy the oppression imposed on women.

The aim of this thesis is to examine the concept of time in Dickens¡¦s novels including A Christmas Carol, Hard Times and A Tale of Two Cities with the newly discovered concepts of time in physics, geology and social economy. I intend to use them to build possible parallels, which will help to clarify the working of time and its influences imposed upon the characters in Dickens¡¦s novels. The thesis, in a sense, is an experiment of cross-boundary study and, through a close examination of the three books, tends to provide an alternative way to rethink the relationship between time, characters, the world and the author in Dickens¡¦s novels.

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This dissertation aims to study three very important works in English children&rsquo / s fiction: C. S. Lewis&rsquo / s The Lion, the Witch and the Wardrobe, Philip Pullman&rsquo / s The Subtle Knife, the second book of his trilogy His Dark Materials, and J. K. Rowling&rsquo / s Harry Potter and the Philosopher&rsquo / s Stone. The novels will be analyzed in terms of their approaches toward the conventions of fairy tale, fantasy and romance / to this end, the novels are to be evaluated in relation to their concept of chronotope, and the quest of good versus evil. While the secondary world or multiple worlds presented are going to be analyzed in terms of their perception of time and space along with the presentation of the supernatural elements, the characters will be evaluated in terms of the common classification good versus evil. The main argument of this study concentrates on the gradual estrangement from the crystal clear distinctions of the fairy tale genre to a more shadowy, pessimistic, and ambivalent vision of the fantastic in the children&rsquo / s literature.

The field of synthetic biology seeks to revolutionize the scope and scale of what is currently feasible by genetic engineering. By focusing on engineering general signal processing platforms using modular genetic parts and devices rather than `one-off' systems, synthetic biologists aim to enable plug-and-play genetic circuits readily adaptable to different contexts. For mammalian systems, the goal of synthetic biology is to create sophisticated research tools and gene therapies. While several isolated parts and devices exist for mammalian systems there are few signal processing platforms available. We addressed this need by creating a transcriptional regulatory framework using programmable zinc finger (ZF) and TALE transcription factors and a conceptual framework for logical T-cell receptor signaling. We first engineered a large set of ZF activator and repressor transcription factors and response promoters. ZFs are scalable elements as they can be engineered to bind to given DNA sequences. We demonstrated that we could ‘tune’ the activity of the ZF transcription factors by fusing them to protein homo-dimerization domains and by modifying their response promoters. We also created OR and NOR logic gates using hybrid promoters and AND and NAND logic gates by reconstituting split zinc finger activators and repressors with split inteins. Next, using a computational algorithm we designed a series of TALE transcriptional activators and repressors predicted to be orthogonal to all 2kb human promoter regions and thus minimally interfere with endogenous gene expression. TALEs can be designed to bind to even longer DNA sequences than ZFs, however off-target binding is predicted to occur. We tested our computationally designed TALEs in human cells demonstrating that they activated their intended target genes, but not their likely endogenous off-target genes, nor synthetic promoters with binding site mismatches. Finally, we created a conceptual framework for logical T-cell-mediated killing of target cells expressing combinations of surface antigens. The systems consist of conventional and novel chimeric antigen receptors (CARs) containing inhibitory or co-stimulatory cytoplasmic signaling domains. In co-incubation assays of engineered T-cells with target cells we demonstrated a functioning OR-Gate system and progress toward development of a functional NOT-Gate system using the CD300a and CD45 inhibitory receptor domains.

The human genome encodes information that instructs human development, physiology, medicine, and evolution. Massive amount of genomic data has generated an ever-growing pool of hypothesis. Genome editing, broadly defined as targeted changes to the genome, posits to deliver the promise of genomic revolution to transform basic science and personalized medicine. This thesis aims to contribute to this scientific endeavor with a particular focus on the development of effective human genome engineering tools.

The dynamic nature of gene expression enables cellular programming, homeostasis and environmental adaptation in living systems. Dissection of causal gene functions in cellular and organismal processes therefore necessitates approaches that enable spatially and temporally precise modulation of gene expression. Recently, a variety of microbial and plant-derived light-sensitive proteins have been engineered as optogenetic actuators, enabling high-precision spatiotemporal control of many cellular functions. However, versatile and robust technologies that enable optical modulation of transcription in the mammalian endogenous genome remain elusive. Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana. LITEs do not require additional exogenous chemical cofactors, are easily customized to target many endogenous genomic loci, and can be activated within minutes with reversibility. LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations. We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications. We explore the modularity of the LITE approach through the development of CRISPR/Cas9 transcriptional effectors in either constitutively active or light-inducible contexts. The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes and enables direct testing of the causal roles of genetic and epigenetic regulation in normal biological processes and disease states. / Engineering and Applied Sciences

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