京都大学 / 0048 / 新制・課程博士 / 博士(人間・環境学) / 甲第19797号 / 人博第768号 / 新制||人||185(附属図書館) / 27||人博||768(吉田南総合図書館) / 32833 / 京都大学大学院人間・環境学研究科共生人間学専攻 / (主査)教授 齋藤 治之, 教授 壇辻 正剛, 准教授 谷口 一美 / 学位規則第4条第1項該当 / Doctor of Human and Environmental Studies / Kyoto University / DGAM

京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第21338号 / 医博第4396号 / 新制||医||1031(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 坂井 義治, 教授 平井 豊博, 教授 溝脇 尚志 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

INTRODUCTION: Previous studies have shown that mammalian expressed CTRP3 functions to lower blood glucose levels in transgenic mice. However, the production of mammalian expressed CTRP3 is expensive and can only yield up to 1mg/L of recombinant protein. On the contrary, bacterial expressed CTRP3 is relatively inexpensive and can theoretically produce 70mg of recombinant protein per L of bacteria. The purpose of this experiment is to see if functional CTRP3 protein can be produced effectively and economically using bacterial expression system. METHODS: The ctrp3 gene was inserted into a bacterial expression plasmid vector and transfected into Escherichia Coli (E. coli) bacterium to produce recombinant Polyhistidine-tagged-CTRP3 in the presence of L-Arabinose. Optimal expression parameters were then tested in the transfected bacteria. Optimal expression was confirmed by measurement of CTRP3 in through immunoblotting. RESULTS: Once optimal expression conditions were established, large amounts of CTRP3 were generated and purified by commercial HIS purification systems. Due to post-translation modification of CTRP3, it is unclear if the purified CTRP3 will be functional. The next step in the process is to optimize the purification protocol and test the functionality of the bacterial expressed CTRP3. Support or Funding Information This research was supported in part by National Institute on Alcohol Abuse and Alcoholism of the National Institutes of Health under Award Number R03AA023612 and East Tennessee State University Research Development Committee (E82262).

Web analytics are used to track and examine user behavior on websites and web applications. In order to take data driven decisions companies and organizations working with the web look to web analytics to understand their users. A piece of Javascript code that collect user behavior and information is often referred to as a tag, which can be managed through a tag management system. Tag management systems can provide structure to how a website’s users’ behaviors are being measured. This study examines how a tag management system can enable web analytics of user data to be incorporated in an agile web development process at the Swedish company Dailybitsof. With a literature study, case study and interviews with professionals on the subject, a set of recommendations to enable web analytics is presented. This study suggests that a tag management system can enable web analytics to be incorporated into an agile web development process if it is implemented in combination with changes to the agile process. / Webbanalys används för att spåra och undersöka användarbeteende på hemsidor och webbapplikationer. För att ta datadrivna beslut, använder sig företag och organisationer av webbanalys för att förstå sina användare. Några rader Javascript-kod kan användas för att samla upp användarbeteende och kallas ibland för ett tag. Dessa tags kan behandlas i ett så kallad tag management-system. Tag management-system kan ge struktur över hur användarbeteendet på en hemsida spåras och analyseras. Den har uppsatsen undersöker hur ett tag management-system kan möjliggöra att data från webbanalys blir använt i en agil webbutvecklingsprocess hos det svenska företaget Dailybitsof. Med en litteraturstudie, fallstudie och intervjuer med professionella inom ämnet presenteras rekommendationer för att att möjliggöra webbanalys. Uppsatsen föreslår att ett tag management-system kan möjliggöra användandet av webbanalys i en agil webbutvecklingsprocess om det implementeras i kombination med ändringar av den agila processen.

В различных САПР были спроектированы различные варианты бесчиповых меток, а так же отдельных компонентов метки. Данные идентификаторы в будущем могут служить заменой других подобных устройств, таких как RFID метки с чипом, штрих - коды и т.д. После оптимизации в программах моделирования были изготовлены различные варианты меток с дальнейшим измерением их характеристик. В работе приведены характеристики, как моделей, так и изготовленных меток. Были предложены варианты использования меток. Актуальность работы определяется, прежде всего, тем, что разрабатываемые устройства очень слабо распространены, но при этом, за счёт своих преимуществ имеют очень большой потенциал к применению. / In different CAD systems were designed various options without chip label and a separate label components. These IDs in the future can serve as a replacement for other similar devices such as RFID tags with chip, bar codes, etc. After the optimization in the simulation programs have been produced with different versions of tags, with further measurement of their characteristics. The paper presents characteristics of both models and made labels. Options were proposed for labeling. The urgency of work is determined, primarily, by the fact that the developed device is very poorly distributed, but its benefits have great potential for use.

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