隨全球經濟發展、環保意識抬頭，以及食品安全衛生議題的日益重視，運用資訊科技維護人們食的安全已是一股不可擋的趨勢。本論文以資訊科技導入農產品生產控管以及維護生產品質、建立所謂農產履歷為研究目的；並以豬隻生產為例，深入探討在豬隻的生產過程中如何導入這新的科技技術，以及所搭配的生產履歷資訊平台。 文中首先針對所謂「農產履歷」作一詳細介紹，其中包括農產履歷的來由、推動農產履歷原因，以及目前各國所推展的農產履歷的現況如何。另外，在導入農產履歷之前也必須先了解所謂的「追溯系統」，在農產品整個生產過程中有哪些東西或流程是必須追蹤記錄。 本研究案在眾多的農產品中選擇以豬隻的生產過程作為導入「農產履歷」的研究標的其原因是因為： 1、 豬隻為我國農產品中產值最高。 2、 豬隻的生產過程短，從出生到送入屠宰僅六個半月。 3、 豬隻的生產過程中所需控管的項目較多，例如：飼料、添加劑、藥品、種源等。 本研究選擇以RFID作為農產履歷的記錄介質，在豬隻的身上打上所謂的RFID晶片，借以控管並記錄豬隻的生產過程，然，RFID晶片的外在形式有兩種，一種是外在耳掛式的晶片，稱之為「RFID耳標」，另ㄧ種是皮下植入式的，我們稱之為「植入式晶片」，本研究亦會針對這兩種不同的晶片同時導入加以評估，選擇一個最適合於豬隻生產所應搭配的形式。 除此之外，搭配RFID晶片所需的生產資訊系統，意即「畜牧場管理系統」也是不可或缺的，本研究案同時也針對了該管理系統有一深入的研究並提出該系統的設計理念，以及相關的系統設計方式，以期在本研究結束後能夠提出一套完整的豬隻生產過程的「農產履歷系統」。 / As the global economic development, environmental awareness and the attention to food safety, applying technology to safeguard people's food security is an irreversible trend. The purposes of this paper are to addresses the methods of introducing technology into agricultural production and control, and the maintenance of production quality. Take the production of pigs for example to investigate the introduction of technology to agricultural production as well as the operation and usage of information platform. The thesis is aimed at so-called “agro-biographical”, containing the origins, the motivations and the current status of introduction of agro-biographical all around the world. On the other hand, the so-called "tracing system" is acknowledged before the introduction of agro-biography, which indicates what and how the detailed data should be included or recorded. The case studies were selected in many agricultural production processes as a force to pigs "agro-biographical" because of the research subjects: 1. Pigs generate the highest value among Taiwan’s agricultural products and they are the necessity. 2. The production process of pigs is short, only six and a half months from birth to slaughter. 3. The more attentions are required to pigs production process and control, such as feed, additives, drugs, plant sources. The study selected RFID as agro-biographical recording media, which control and record pig production process. These RFID chips are in two forms, one is the external ear TAGs, the so called “RFID ear TAG”, and the other is in subcutaneous implantation style in the heads, known as “Subcutaneous Implantation TAG”. The study made comparison between these two kinds of RFIDs and the evaluated them to choose the better one. In addition, the mix of RFID chips for the production of information systems, namely, “The livestock management system” is also indispensable. The research also investigated in the management system , concepts of design, and related systems topics. Finally, the results are hoped to make contribution to the introduction of technology to agro-biography.

The Company Siemens Industrial Turbo Machinery AB manufactures and launches in operation among other things gas turbines, steam turbines, compressors, turn-key power plants and carries out service for components for heat and power production. Siemens also performs research and development, marketing, sales and installations of turbines and completes power plants, service and refurbish. Our thesis for the engineering degree is to develop an automatic status log which will be used as a tool to control how the status of the machine is before and after technical service at gas turbines. Operational disturbances will be registered in a structured way in order to get a good possibility to follow up the reliability of the application. An automatic log function has been developed and will be activated at start, stop and shutdown of the turbine system. Log files are created automatically and get a name with the event type, the date and the time. The files contain data as timestamp, name, measured values and units of the signals which are going to be analyzed by the support engineers. They can evaluate the cause of the problem using the log files.

Le but de cette thèse est de développer des capteurs sur la base des tags RFID, des technologies et matériaux disponibles au Vietnam afin de contribuer à résoudre la problématique du contrôle de la qualité des produits alimentaires. En effet la technologie RFID s’est affirmée en importance pour ses applications dans de nombreux domaines. Dans ce contexte, l’identification des produits alimentaires expirés, sans les endommager, est une orientation de recherche très prometteuse. Un tag RFID UHF passif peut aussi être composé de plusieurs puces et plusieurs antennes, chaque couple puce/antenne conçu pour travailler sur un intervalle déterminé de valeur de permittivité. Donc, à partir de l’ensemble des permittivités définies pour chaque couple puce/antenne et les signaux réfléchis vers le lecteur, nous pourrons «mesurer» la permittivité de l’objet tracé. Ainsi la connaissance de la permittivité des aliments et la conception spécifique de l’antenne, nous développerons un tag capteur de type "multi puce/antenne" qui sera utilisé comme un capteur sans fil pour la détection de la qualité des aliments / In recent years, RFID technology has established itself in importance, particularly for applications in the civil sector. In this context, identification of expired products without damage is a very promising direction of research. However, the price of these sensors is still too high especially compared to living in Vietnam. A passive UHF RFID tag chip can include many antennae and many chips on a same substrate in which each pair of chip/antenna is designed to be activated on a determined interval value of permittivity. So from designed permittivities for each pair of chip/antenna and the reflected signals to the reader, we can define the value of permittivity of the object that is labelled with RFID tag. From the characterization of food permittivity and the background of antenna design, we developped a sensor tag "multi chip/antenna" to be used as a wireless sensor for the detection of food quality. The aim of this thesis intends to develop a new family of wireless sensors based on RFID technology and available technology of fabrication in Vietnam to solve this problem.

Made available in DSpace on 2015-03-26T13:42:23Z (GMT). No. of bitstreams: 1 texto completo.pdf: 636309 bytes, checksum: ca1e7c510537c62820f228b759c5a23d (MD5) Previous issue date: 2011-07-25 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O suíno (Sus scrofa) é considerado um animal de grande importância para produção de carne, sendo seu potencial de crescimento muscular objeto de grande interesse e geralmente associado com características determinadas na fase pré-natal durante a miogênese. Para o estudo de genes responsáveis por estas características, as etiquetas de sequências expressas (Expressed Sequence Tags - EST) fornecem informações diretas sobre o transcriptoma e indiretas sobre a relação entre o genoma e diferentes fenótipos, proporcionando o conhecimento sobre genes diferencialmente expressos (GDE) bem como sequências genômicas transcritas para o controle da expressão gênica como, por exemplo, alguns RNAs não codificantes. Características de tecidos musculares em suínos podem ser influenciadas diretamente por genes, e estes sendo regulados como, por exemplo, através de miRNAs, em diferentes fases de desenvolvimento. O presente trabalho teve como objetivo a identificação e a anotação in sílico de GDE e sequências não codificantes, com enfoque aos miRNAs, de bibliotecas de cDNA construídas a partir do músculo esquelético semi-membranoso de três diferentes raças de suínos (Duroc, Large White e naturalizada brasileira Piau) bem como a análise dos níveis de expressão dos genes identificados e miRNAs em sete fases de desenvolvimento do Longissimus Dorsi (21, 40, 70 e 90 dias pré-natal e 107, 121 e 171 dias pós-natal) de animais de linha comercial. Foram identificados 34 GDE sendo 21 pertencentes a uma rede gênica musculo-específica. Destes, 13 genes tiveram seus perfis de expressão analisados com o uso do qRT-PCR durante os sete períodos citados, formando quatro grupos de expressão semelhantes, um com maior expressão na fase pós-natal e três na fase pré-natal. Nas análises das sequências não codificantes um resultado importante foi a identificação de dois novos miRNAs em suínos, os quais tiveram suas sequências maduras similares aos miRNAs hsa-miR-1207-5p e hsa-miR-665 foram classificadas como verdadeiras pelo programa MiPred e formaram estruturas secundárias. Destes, encontrou-se 289 e 214 genes regulados por eles respectivamente, dos quais quatro são músculo-específicos. Os novos miRNAs tiveram seus perfis de expressão analisados com o uso do PCR em tempo real durante os sete períodos citados juntamente com outros três já identificados em suínos. Seus níveis de expressão mostraram diferenças entre os estágios pré- e pós-natal. Estes estudos podem fornecer valiosas informações possibilitando um maior entendimento dos mecanismos moleculares envolvidos no desenvolvimento muscular. As análises de GDE em fases pré e pós-natal sugerem a presença de genes atuando especificamente em determinados estágios de desenvolvimento do músculo, contribuindo para melhor explicar suas funções. A identificação de dois novos miRNAs, somados a outros já identificados e postados nos bancos de dados em suínos, podem contribuir para um maior entendimento dos modos de regulação gênica, sendo de importância para os estudos de genética e melhoramento animal, permitindo o entendimento da fisiologia da deposição de músculo para produção de carne em suínos. / The pig (Sus scrofa) is considered an important animal for meat production. This interest revolves around the potential for muscle growth, which usually is associated with certain characteristics during prenatal myogenesis. To study the genes responsible for these characteristics, expressed sequence tags (EST) provide direct information about the transcriptome and indirectly on the relationship between the genome and different phenotypes, supplying knowledge about differentially expressed genes (DEG) as well as other transcribed genomic sequences for the control of gene expression, e.g., some non-coding RNAs. Characteristics of muscle tissue in pigs may have been directly influenced by genes, and those being regulated, for example, by miRNAs, in different stages of development. This study aimed to identify by in silico annotation, DEG and non-coding sequences, focusing on miRNAs, using cDNA libraries constructed from semi-membranous skeletal muscle of three different pig breeds (Duroc, Large White and naturalized Brazilian Piau ) as well as analysis of gene expression profiles of identified genes and miRNAs during seven stages of development (21, 40, 70 and 90 days prenatal and 107, 121 and 171 days postnatal) from commercial line animals Longissimus Dorsi muscle. Twenty-one identified genes out of 34 DEGs belongs to the muscle-specific path. From these, 13 genes had their expression profiles analyzed by qRT-PCR during the seven periods, forming four clusters of similar expression, with one having greater expression in the postnatal period and three in the prenatal. In the analysis of non-coding sequences, an important result was the identification of two new miRNAs in pigs, which had their sequences similar to mature miRNAs hsa-miR-1207- 5p and hsa-miR-665 which had their precursor sequences forming secondary structures and classified as real precursor sequence by MiPred program. From these, we found 289 genes and 214 respectively regulated by them, of which four are muscle-specific. The new miRNAs and other three which have been identified in previous studies in pigs had their expression levels analyzed by quantitative real time PCR during the mentioned seven periods. Their levels of expression differed between pre-and postnatal stages. These studies may provide valuable information allowing a better understanding of the molecular mechanisms involved in muscle development. Analyses of DEG in the pre-and postnatal periods suggest the presence of genes acting specifically on certain stages of muscle development, contributing to better explain their functions. The identification of two new miRNAs, together with other previously identified and posted on the databases in pigs, may contribute to a better understanding of gene regulation and is important for studies of genetics and animal breeding, allowing the understanding of the muscle deposition physiology to meat production in pigs.

Made available in DSpace on 2016-12-08T16:19:08Z (GMT). No. of bitstreams: 1 PEDRO.pdf: 1575259 bytes, checksum: dd85d138802259660db83681f67418cf (MD5) Previous issue date: 2011-02-24 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Rolê, in urban slang means to walk around, to go for a walk. Following this definition, this research walks through files posted by urban writers, in the great computer‟s world wide net. The technological advance allowed great changes in the way we communicate to each other. The spaces for human relationship have spread, and are also cyberspace and the rolê now can be digital. To comprehend this dive in the virtual, present in every generation of the XXI century, it is necessary to get to know the origins of the internet e also concepts which refer to the virtual space and the changes in communication towards the digital technological means. The approaches presented here depart from historicities, one about the tags/pixos/graffiti and, in parallel, about the great virtual net. in the arts field, before the internet, some artist developed a world wide connection of files exchange, through the post; highlighted here the Fluxus group, as one of the pioneers. Among innumerous files, pieces of art, photos and cards circulated, which provoked ruptures and a series of transformations in the art institutions and allowed a historical record. In this flow of postal, conceptual, art, the work of Cildo Meireles and Paulo Bruscky consolidated, in Brazil, an artistic production from autonomic spaces, and promoted alternatives circuits of information. Previous to the internet, this artists published their pieces with political and symbolic information and, even towards a military regime, it was possible the circulation of the information among the social environment, using the mass media channels and the traditional exchange systems. In the same way, the urban writers, autonomous, made the polyphonies circulate the digital using the great net as an information exchange channel, as a great advertising, activism and political protagonism tool / Rolê na gíria urbana quer dizer caminhar, dar uma volta. Seguindo esta definição, esta pesquisa caminha por arquivos postados pelos escritores urbanos, nas vias da grande rede mundial de computadores. O avanço tecnológico permitiu grandes mudanças na maneira de nos comunicarmos. Os espaços de relacionamento humano se estenderam, e também são ciberespaço e o rolê agora pode ser também digital. Para compreender este mergulho no virtual, presente em toda a geração do século XXI, é necessário conhecer a origem da internet e também conceitos referentes ao espaço virtual e às mudanças na comunicação diante dos meios tecnológicos digitais. As abordagens aqui apresentadas partem de historicidades, uma sobre os tags/pixos/graffiti e, paralelamente, sobre a grande rede virtual. No campo das artes, anteriores a internet, alguns artistas desenvolveram uma conexão mundial de troca de arquivos, por meio do correio; destaca-se aqui o grupo Fluxus como um dos pioneiros. Dentre os inúmeros arquivos, circulavam obras de artes, fotografias e cartões, que provocaram rupturas e devires nas instituições das artes e possibilitaram um registro histórico. Nesse fluxo da arte postal, conceitual, o trabalho de Cildo Meireles e Paulo Bruscky consolidou, no Brasil, uma produção artística a partir de espaços autônomos, e promoveu circuitos alternativos de informação. Precursores à internet, esses artistas publicaram suas obras com informações de caráter político e simbólico e, mesmo diante de um regime militar, foi possível a circulação da informação pelo meio social, usando os próprios canais de mass media e os sistemas tradicionais de troca. Da mesma forma os escritores urbanos, autônomos, fizeram circular suas polifonias pelo digital usando a grande rede como um canal de troca de informação, como uma grande ferramenta publicitária, ativismo e protagonização política

Nous présentons dans cette thèse un système de correction automatique d'annotations (tags) fournies par des utilisateurs qui téléversent des vidéos sur des sites de partage de documents multimédia sur Internet. La plupart des systèmes d'annotation automatique existants se servent principalement de l'information textuelle fournie en plus de la vidéo par les utilisateurs et apprennent un grand nombre de "classifieurs" pour étiqueter une nouvelle vidéo. Cependant, les annotations fournies par les utilisateurs sont souvent incomplètes et incorrectes. En effet, un utilisateur peut vouloir augmenter artificiellement le nombre de "vues" d'une vidéo en rajoutant des tags non pertinents. Dans cette thèse, nous limitons l'utilisation de cette information textuelle contestable et nous n'apprenons pas de modèle pour propager des annotations entre vidéos. Nous proposons de comparer directement le contenu visuel des vidéos par différents ensembles d'attributs comme les sacs de mots visuels basés sur des descripteurs SIFT ou des motifs fréquents construits à partir de ces sacs. Nous proposons ensuite une stratégie originale de correction des annotations basées sur la fréquence des annotations des vidéos visuellement proches de la vidéo que nous cherchons à corriger. Nous avons également proposé des stratégies d'évaluation et des jeux de données pour évaluer notre approche. Nos expériences montrent que notre système peut effectivement améliorer la qualité des annotations fournies et que les motifs fréquents construits à partir des sacs de motifs fréquents sont des attributs visuels pertinents / This thesis presents a new system for video auto tagging which aims at correcting the tags provided by users for videos uploaded on the Internet. Most existing auto-tagging systems rely mainly on the textual information and learn a great number of classifiers (on per possible tag) to tag new videos. However, the existing user-provided video annotations are often incorrect and incomplete. Indeed, users uploading videos might often want to rapidly increase their video’s number-of-view by tagging them with popular tags which are irrelevant to the video. They can also forget an obvious tag which might greatly help an indexing process. In this thesis, we limit the use this questionable textual information and do not build a supervised model to perform the tag propagation. We propose to compare directly the visual content of the videos described by different sets of features such as SIFT-based Bag-Of-visual-Words or frequent patterns built from them. We then propose an original tag correction strategy based on the frequency of the tags in the visual neighborhood of the videos. We have also introduced a number of strategies and datasets to evaluate our system. The experiments show that our method can effectively improve the existing tags and that frequent patterns build from Bag-Of-visual-Words are useful to construct accurate visual features

Les symbioses rhizobienne et mycorhizienne à arbuscules sont deux endosymbioses racinaires jouant des rôles importants dans le développement des plantes en améliorant leur nutrition minérale. Les lipo-chitooligosaccharides (LCOs), produits par les bacteries Rhizobia et les champignons mycorhiziens, sont essentiels pour l'établissement de la symbiose rhizobienne et stimulent la mycorhization. Chez la légumineuse Medicago truncatula, trois récepteurs-like kinase à motifs lysin (LysM), LYR3, NFP et LYK3 sont impliqués dans la perception des LCOs. Le travail présenté a eu pour objectif la caractérisation biochimique de ces récepteurs et leurs applications potentielles. Les orthologues de LYR3 de M. truncatula ont été clonés et se sont tous révélés, à l'exception de celui du lupin, capables d'établir une interaction d'affinité élevée avec les LCOs mais pas avec les chitooligosaccharides de structure apparentée. Afin de mieux comprendre les bases moléculaires de la reconnaissance des LCOs, des échanges de domaine entre les protéines LYR3 de lupin et de Medicago ont été effectués et ont révélé l'importance du troisième domaine LysM dans l'interaction. L'exploitation des capacités de reconnaissance des LCOs par LYR3 à des fins biotechnologiques a été évaluée à l'aide de récepteurs chimériques constitués du domaine extracellulaire de LYR3 et du domaine kinase des récepteurs immunitaires AtCERK1 et EFR. Il est apparu que LYR3 peut être utilisé pour élaborer des récepteurs chimériques mais leur mode d'activation reste à optimiser. Enfin l'étude des deux récepteurs symbiotiques NFP et LYK3 suggère qu'ils sont régulés par phosphorylation suite au traitement par les signaux symbiotiques. L'ensemble de ce travail apporte un éclairage nouveau sur les mécanismes de perception des LCOs et sur les modifications associées à leurs récepteurs qui en résultent. / Arbuscular mycorrhization and rhizobial nodulation are two major root endosymbioses which play important roles in plant development by improving their mineral nutrition. Produced by Rhizobia bacteria and mycorrhizal fungi, lipo-chitooligosaccharides (LCOs) were shown to be essential for the formation of the rhizobial symbiosis and to have stimulatory effects on mycorrhization. In the legume Medicago truncatula three lysin motif (LysM) receptor-like kinases LYR3, NFP and LYK3 have been shown to be involved in LCO perception. Here work is presented aimed at the biochemical characterization and application of these important receptor proteins. Cloned from several legume species orthologs of M. truncatula LYR3, except from lupin, were shown to bind LCOs with high affinity, but not structurally-related chitooligosaccharides (COs). Domain swaps between the lupin and Medicago proteins were used as a tool to decipher the molecular basis of LCO recognition and revealed the importance of the third LysM domain for LCO binding. The possibility of exploiting the LCO-binding capacity of LYR3 in biotechnology, through the composition of chimeric receptors, was investigated by combining together the extracellular domain of LYR3 protein with the kinases of Arabidopsis thaliana immune receptors, AtCERK1 and EFR. The results suggest that LYR3 could be used for constructing biologically active chimeric proteins whose mode of activation needs to be improved. Finally studies on the two LysM symbiotic receptors NFP and LYK3 suggest that they are regulated by changes in their phosphorylation after symbiotic treatments. Together this work brings light on the mechanisms underlying LCO perception and the modifications that receptors undergo after their treatment with LCO.

The performance of commercial Radio Frequency Identification (RFID) tags is primarily limited by present techniques used for tag antenna design. Currently, industry techniques rely on identifying the RFID tag application (books, clothing, etc.) and then building antenna prototypes of different configurations in order to satisfy minimum read range requirements. However, these techniques inherently lack an electromagnetic basis and are unable to provide a low cost solution to the tag antenna design process. RFID tag performance characteristics (read-range, chip-antenna impedance matching, surrounding environment) can be very complex, and a thorough understanding of the RFID tag antenna design may be gained through an electromagnetic approach in order to reduce the tag antenna size and the overall cost of the RFID system. The research presented in this thesis addresses RFID tag antenna design process for passive RFID tags. With the growing number of applications (inventory, supply-chain, pharmaceuticals, etc), the proposed RFID antenna design process demonstrates procedures to design tag antennas for such applications. Electrical/geometrical properties of the antennas designed were investigated with the help of computer electromagnetic simulations in order to achieve optimal tag performance criteria such as read range, chip-impedance matching, antenna efficiency, etc. Experimental results were performed on the proposed antenna designs to compliment computer simulations and analytical modelling.

This paper describes the way to realization such an application, where a user chooses a photo database to working with and enters a photo into the system. The system using a visual vocabulary finds the most similar photos from the database and offers tags of the searched photo with a suitable form based on the tag statistical analysis of this photo.

The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. In an attempt to overcome this hurdle, the Escherichia coli (E. coli) cell division protein FtsZ is in this project fused to a nanotag (NT) that is subsequently labelled with a nanobody (NB). The ALFA-tag, a short amino acid peptide, is chromosomally fused to the target protein, creating a MG1655/FtsZ-ALFA strain where all FtsZ proteins have an ALFA-tag attached. Recognising the ALFA-tag is the NB αALFA (anti-ALFA) which is fused to a FP and expressed from a plasmid. The MG1655/FtsZ-ALFA strain is labelled using standard plasmid transformation which allows for live cell imaging of the division ring in E. coli. Both FPs sfGFP and mEos3.2 are used for labelling which means that the cells can be imaged in epifluorescence microscopy and single-molecule Photo-Activated Localisation Microscopy (PALM), and even single-molecule time lapses of the constricting FtsZ-ring is possible. This system is also applicable to other bacterial proteins. / Superupplösningsmikroskopi (SUM) är ett viktigt redskap för att förstå de inre processerna i en bakteriecell. Att på ett framgångsrikt sätt tagga målproteinerna har dock visat sig vara en utmaning för SUM. Att direkttagga målproteinerna med fluorescerande protein är oftast inte möjligt på grund av de fluorescerande proteinernas storlek och traditionell märkning med antikroppar skapar ett för stort avstånd mellan fluorofor och målprotein. För att överkomma detta problem taggas här celldelningsproteinet FtsZ iEscherichia coli (E. coli) med hjälp av nanotaggar (NT) och nanokroppar (NK). ALFA-taggen, en kort aminosyrapeptid, är kromosomt bunden till FtsZ i cellinjen MG1655/FtsZ-ALFA, så att varje FtsZ protein som produceras har en ALFA-tag bunden till sig. NK αALFA (anti-ALFA) känner igen och binder till ALFA-taggen när de kommer i kontakt. NK är bunden till ett fluorescerande protein och uttryckt från en plasmid vilket gör att MG1655/FtsZ-ALFA kan bli taggad med hjälp av vanlig plasmidtransformation. Denna metod möjliggör mikroskopi av divisionsringen i levande E. coli-celler. Två olika fluorescerande protein används, sfGFP och mEos3.2, vilket innebär att både epifluorensmikroskopi och fotoaktiverad lokaliseringsmikroskopi (PALM) kan användas. Dessutom är även intervallfotografering i enmolekylmikroskopi av divisionsringens konstriktion möjligt. Denna märkningsteknik är vidare applicerbar på andra bakteriella protein.