Omega-3 fatty acids, particularly docosahexaenoic (DHA) and eicospentaenoic acid (EPA), are important mediators for cardiovascular disease, fetal/infant development, neurological disorders and inflammatory diseases. Supplementation and washout studies are important for future research on the physiological effects of omega-3 fatty acids and for determination of the proper washout period for future cross-over studies. In this study, omega-3 fatty acid blood biomarker comparisons are made for the n-3 HUFA score (% of n-3 HUFAs in total HUFAs) and omega-3 index (sum of EPA + DHA) in plasma, erythrocytes, whole blood and a novel finger-tip prick blood method (FTPB) of analysis. This FTPB method of fatty acid analysis is further tested to determine the potential for its use in fatty acid analysis. In addition, gender differences in response to omega-3 fish oil supplementation are analyzed in all four blood fractions. Nine males and seven females were supplemented with 8 fish-oil capsules per day (providing 3.2 g/day EPA and 1.6 g/day DHA) for four weeks, followed by an eight-week omega-3 washout period. Venous plasma, erythrocyte and whole blood samples were collected during weeks 0, 4, 8 and 12 and FTPB samples were collected weekly during supplementation and washout fatty acid analysis was performed. EPA and DHA incorporation is lowest in magnitude in erythrocytes relative to all other blood fractions. Omega-3 blood biomarker comparisons demonstrate that the n-3 HUFA score is a more reliable measure across all blood fractions compared to the omega-3 index. In addition, the n-3 HUFA score demonstrates no differences (p > 0.05) between FTPB and whole blood analysis, providing evidence to support its usefulness as a tool for fatty acid analysis. However, differences (p < 0.05) do exist between these methods for saturated fatty acid, monounsaturated fatty acids, omega-6 polyunsaturated fatty acids (PUFAs) and omega-3 PUFAs. Baseline fatty acid levels for DHA, and the DHA:EPA and DHA:DPA ratios tend to be higher (p < 0.05) in females, and docosapentaenoic acid n-3 (DPAn-3) is higher (p > 0.05) in males across all blood fractions. Furthermore, a gender effect (p < 0.05) is seen for the DHA:EPA ratio across all blood fractions. At baseline, female DHA:EPA is higher (p < 0.05) than males with supplementation lowering both male and female values and removing any differences (p > 0.05) between genders. Washout results in a return of levels towards baseline, however, baseline levels are not fully reached. Furthermore, while gender differences do begin to reform during washout, these differences are not significant (p > 0.05). In conclusion, omega-3 fatty acid responses, particularly DHA:EPA ratio, demonstrate significant gender differences that may be related to differences in long-chain PUFA synthesis pathways between males and females. In addition, the n-3 HUFA score may be a more valuable omega-3 blood biomarker than the omega-3 index, as the n-3 HUFA score displays more consistent levels across all blood fractions. Finally, the FTPB method of analysis may be a useful tool in the measurement of fatty acid composition, however, some microwave methylation problems do exist, specifically in the phospholipid class of lipids.

Aeroacoustic investigations regarding different types of helicopter noise have indicated that the most annoying noise is caused by impulsive blade surface pressure changes in descent or forward flight conditions. Blade Vortex Interaction (BVI) is one of the main phenomena producing significant impulsive noise by the unsteady fluctuation in blade loading due to the rapid change of induced velocity field during interaction with vortices shed from previous blades. The tip vortex core structure and the blade vortex miss distance were identified as having a primary influence on BVI. In this thesis, piezoelectrically modulated and/or vectored blowing at the rotor blade tip is theoretically investigated as an active technique for modifying the structure of the tip vortex core as well as for increasing blade vortex miss distance. The mechanisms of formation and convection of rotor blade tip vortices up to and beyond 360 degrees wake age are described based on the CFD results for the baseline cases of a hovering rotor with rounded and square tips. A methodology combining electromechanical and CFD modeling is developed and applied to the study of a piezoelectrically modulated and vectored blowing two-dimensional wing section. The thesis is focused on the CFD analysis of rotor flow with modulated tangential blowing over a rounded blade tip, and with steady mid-plane blade tip blowing, respectively. Computational results characterizing the far-wake flow indicate that for steady tangential blowing the miss distance can be doubled compared to the baseline case, which may lead to a significant reduction in BVI noise level if this trend shown in hover can be replicated in low speed forward flight. Moreover, near-wake flow analysis show that through modulated blowing a higher dissipation of vorticity can be obtained.

Intermittent contact mode atomic force microscopy has been widely employed for simultaneous topography imaging and material characterization. The work in the literature includes both qualitative and quantitative methods. Regular AFM cantilevers are generally used in these methods, yet these cantilevers come with certain limitations. These limitations result from the very nature of cantilever probes. They are passive force sensors with insufficient damping. This prevents having active and complete control on tip-sample forces, causing sample damage and inaccurate topography measurement. Ideally, an AFM probe should offer high bandwidth to resolve interaction forces, active control capability for small interaction force and stable operation, and sufficient damping to avoid transient ringing which causes undesired forces on the sample. Force sensing integrated readout and active tip (FIRAT) probe offers these properties. A special imaging mode, time-resolved interaction force (TRIF) mode imaging can be performed using FIRAT probe for simultaneous topography and material property imaging. The accuracy of topography measurement of samples with variations in elastic and adhesive properties is investigated via numerical simulations and experiments. Results indicate that employing FIRAT probe's active tip control (ATC) capability during TRIF mode imaging provides significant level of control over the tip-sample forces. This improves the accuracy of topography measurement during simultaneous material property imaging, compared to conventional methods. Moreover, Active tip control (ATC) preserves constant contact time during force control for stable contact while preventing loss of material property information such as elasticity and adhesive forces.

<p>The objective of this thesis is to develop a model that represents the dynamics of a hydraulically operated forestry crane. The model was derived with the traditional Euler-Lagrange formalism and considers the crane mechanics, three double-acting hydraulic cylinders and the valve control unit. On the basis of the derived model we reproduced the entire crane model in MATLAB in order to run simulations herewith. This gave us the possibility to do parameter changes for further studies of the crane in motion.</p><p>Another major goal within the thesis work was to estimate cylinder friction of the hydraulic actuators. We built up a test rig and used double-acting cylinders for determing their frictional behaviour. For this, we ran open-loop experiments in order to create velocity-friction maps that represented the static friction force of the cylinders. In this concern, we varied system pressure and cylinder load to study their influence on the friction force. By means of the derived static friction maps we approached the cylinder’s dynamic friction behaviour and applied both step and ramp control inputs to examine the spring-damping characteristics of the microspoic bristles in the contacting area. The dynamic friction experiments have been exerted in the fashion of the LuGre model. As a result we acquired different nominal friction parameters that we necessarily used to develope adequate friction models.</p><p>A third objective of this thesis was to establish a crane-tip control. Instead of a traditional control, providing a direct relationship between joystick input and cylinder extension, the focus was to build up a control for the end-effector’s trajectory in a two-dimensional frame. This could be achieved by using inverse kinematics in order to determine the required joint angles that corresponded to the desired position of the crane-tip.</p><p>The work also contains a CD including all developed MATLAB models that have been written within this project.</p>

<p>Det här rapporten är en del av mitt examensarbete för högskoleingenjörsexamen i elektroteknik vid Växjö Universitet, MSI. Arbetet är en del av ett större projekt kallat Kranstyrningsprojektet som bedrivs av Växjö Universitet, Rottne Industri med flera, vars syfte är att underlätta för förare att kontrollera den kran som finns på Rottnes skotare. För projektet har det använts en laborationskran och nu har önskemålet varit att det till kranen ska installeras ett nytt styrsystem från dSPACE. Dessutom vill man att nya givare ska sättas på kranen och kopplas till dSPACE. Man vill även ha en manual för att nya studenter ska kunna använda systemet. Det nya dSPACE-systemet består av en datorlåda med processor- och I/O-kort. Man kommunicerar med dSPACE från en PC. Arbetet med installationen har stött på flera problem och arbetet har dragit ut på tiden. När systemet till slut gick att använda utfördes ett par tester och sen implementerades det på kranen. Arbete med att ta fram underlag för installation av accelerometrar på kranen pågick samtidigt och en jämförelse mellan olika alternativ gjordes. Arbetet resulterade i att laborationskranen nu går att styra med det nyinköpta systemet från dSPACE och en manual skapades för att underlätta användandet av systemet.</p> / <p>This document is a part of my bachelor degree thesis in electronic engineering was done at Växjö University, MSI. This work is a part of a bigger project called Crane Tip Control which is pursued by Växjö University, Rottne Industri and others, whose purpose is to facilitate for drivers to control the crane used on forwarders from Rottne. For this project, a laboratory crane has been used and they requested that a new control system from dSPACE was installed to the crane. Also they want new sensors to be placed on the crane and connected to dSPACE. A manual should also be created for new students who want to use the system. The new dSPACE-system consists of a computer box with processor- and I/O-boards. Communication with dSPACE is done via a PC. The work with the installation has encountered many problems and the work has been overrun. When the system finally was working, some tests were performed and then the system was installed at the crane. A task to assemble data for installation of accelerometers, was done during the same time and a comparison between different alternatives was made. The work resulted in the laboratory crane is now able to be controled by the new dSPACE-system and a manual was created to facilitate the usage of the system.</p>

Spinal cord injury induces anatomical plasticity throughout the nervous system, including distant locations in the brain. Several types of injury-induced plasticity have been identified, such as neurite sprouting, axon regeneration and synaptic remodeling. However, the molecular mechanisms involved in anatomical plasticity after injury are unclear, as is the extent to which injury-induced plasticity in the brain is conserved across vertebrate lineages. Here, I used lampreys to identify the molecular mechanisms in mediating anatomical plasticity, because lampreys undergo anatomical plasticity and functional recovery after a complete spinal cord transection. Due to their robust roles in neurite outgrowth during neuronal development, I examined synapsin and synaptotagmin for their potential involvement in anatomical plasticity after injury. I found increased synapsin I mRNA throughout the lamprey brain as well as increased protein levels of synapsin I, phospho-synapsin (Ser 9) and synaptotagmin in the lamprey hindbrain after injury, suggestive of anatomical plasticity. Anatomical plasticity was confirmed at the ultrastructural level, where I found increased neurite density in the lamprey hindbrain after injury. Other molecular mechanisms that promote anatomical plasticity have been previously identified, such as cyclic AMP (cAMP). However, the cellular mechanisms and the molecular targets of cAMP in mediating anatomical plasticity are unclear. My investigation of cAMP revealed that cAMP enhanced the number of regenerated axons beyond the lesion site in lampreys after injury. For the first time in a spinal cord injury model, I found cAMP prevented the death of axotomized neurons that normally have a high tendency to die after injury. In addition, cAMP promoted more regenerating axons to re-grow in straighter paths rather than turning rostrally towards the brain stem. At the molecular level, I found cAMP increased synaptotagmin protein level at the regenerating axon tips, suggestive of enhanced axon elongation. Taken together, my results show that neurite sprouting in the brain and the cAMP-enhanced axon regeneration are conserved responses in vertebrates after spinal cord injury. In addition, my results suggest that at least some developmental pathways are activated during injury-induced and cAMP-enhanced anatomical plasticity. Further understanding of these pathways will provide insights for improving recovery after spinal cord injury. / text

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Invasive growth through a substrate requires a massive amount of penetrative force, and this is generated in the space of a few microns in a growing tip. This process is known to be critical in the root hair, pollen tube, rhizoids, and the topic of this thesis, hyphal growth. However defining the mechanisms underlying the tip growth remains a contentious issue. Shortcomings in control of direction and regulation of growth began to undermine early turgor-based theories, and the cytoskeletal protein actin, ubiquitous in nature and with crucial roles in structure and motility became a target for investigation. A major breakthrough came with the discovery that a characteristic actin depleted zone (ADZ) occurs at the growing tip of hyphae during invasive but not non-invasive hyphal growth. The ADZ is likely to have an important role in generating the greater protrusive force required for invasive growth. However, since its discovery, little has been determined about the characteristics of the ADZ. Uncertainty in the description of the physical environment the hyphae face adds a layer of complexity to interpretation of results. This thesis aims to address this issue, studying the impact of increasing agarose substrate concentration on the presence and dimensions of the ADZ in the oomycete A. bisexualis. Furthermore, agarose is examined by compression and imaging to compare the physical characteristics of the agar samples over the range of concentrations, and determine whether increasing agarose concentration influences agarose gel structure. Results suggest a difference in the number of ADZ observed in non-invasive compared with invasive samples, however no significant differences in the number or dimensions of ADZ were found amongst the 1-4% w/v agarose concentrations. The 0% sample showed 20.7 percent of hyphae exhibited depleted zones, while 1, 2, 3 and 4% samples showed 56.9%, 48.8%, 40.9% and 54.2% respectively. ADZ dimensions did not correlate with agarose concentration. The average ADZ area:hyphal diameter ratio was 0.634, 0.526, 0.430, 1.09, and 0.65 for 0-4% agarose concentrations respectively. Additionally, investigation of gel compression forces revealed gel strength increases with agarose concentration. The force required to compress the agarose increased from 1.85 Psi in 1% agarose to 4.85, 7.09 and 12.22 Psi in 2, 3 and 4% agarose concentrations respectively. SEM imaging, however, suggests heterogeneity of the fibrous interconnected network of agarose gels at a microscopic scale with variable porous structure at all agarose concentrations. This scale is relevant to hyphal tip growth. In combination, these results suggest F-actin depletion may be a response mechanism to provide greater force for invasive growth. Additionally, this response is not dependent on the concentration of the agarose media, possibly due to the variability encountered within the media. These results contribute another important step forward in unraveling the elusive mechanism of tip growth.

The interaction of an evanescent wave and plasmonic nanostructures are simulated in Finite Element Method. Specifically, the optical absorption cross section (Cabs) of a silver nanoparticle (AgNP) and a gold nanoparticle (AuNP) in the presence of metallic (gold) and dielectric (silicon) atomic force microscope (AFM) probes are numerically calculated in COMSOL. The system was illuminated by a transverse magnetic polarized, total internally reflected (TIR) waves or propagating surface plasmon (SP) wave. Both material nanoscale probes localize and enhance the field between the apex of the tip and the particle. Based on the absorption cross section equation the author was able to demonstrate the increment of absorption cross section when the Si tip was brought closer to the AuNP, or when the Si tip apex was made larger. However, the equation was not enough to predict the absorption modification under metallic tips, especially for a AgNP's Cabs; neither it was possible to estimate the optical absorption based on the localized enhanced field caused by a gold tip. With the help of the driven damped harmonic oscillator equation, the Cabs of nanoparticles was explained. In addition, this model was applicable for both TIR and Surface Plasmon Polaritons illuminations. Fitting the numerical absorption data to a driven damped harmonic oscillator (HO) model revealed that the AFM tip modifies both the driving force (F0), consisting of the free carrier charge and the driving field, and the overall damping of the oscillator beta. An increased F0 or a decreased beta will result in an increased Cabs and vice versa. Moreover, these effects of F0 and beta can be complementary or competing, and they combine to either enhance or suppress absorption. Hence, a significantly higher beta with a small increment in F0 will result in an absorption suppression. Therefore, under a Si tip, Cabs of a AuNP is enhanced while Cabs of a AgNP is suppressed. In contrast, a Au tip suppresses the Cabs for both Au and Ag NPs. As an extension of this absorption model, further investigation of the guided mode and a close by nanostructure is proposed, where the scattered wave off the structure attenuates the guided mode with destructive interference.

Mobile boom cranes are used throughout the world to perform important and dangerous manipulation tasks. Given their mobility, these types of cranes can quickly be moved into position. Generally, their base is then fixed and stabilized before they start lifting heavy materials. The usefulness of these cranes can be greatly improved if they can utilize their mobile base during the lifting and transferring phases of operation. This ability greatly expands the workspace by combining base motion with the rotation, lifting, and luffing motions. Of course, the cranes lose some stability margin when a payload is attached. The stability is further degraded when the payload swings. This Master's Thesis presents a stability study of such cranes. As a first step, a static stability analysis of a boom crane is conducted in order to provide basic insights into the effects of the payload weight and crane configuration. Then, a semi-dynamic method is used to take the payload swing into account. As a final step, the results of a dynamic stability analysis obtained by using a multi-body simulation of the boom crane are compared to the outcomes of the previous approaches. This provides conclusions for the practical application of stability analysis. A control method that limits payload swing, and thereby improves stability, is also presented.