The increasing number of Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli (E. coli) associated with sepsis in China is the reason for designing the current study. During 2014-2016, thirty hospitals representing 10 different provinces in China was involved in collecting E. coli isolates causing blood stream infections. Early treatment with suitable antibiotics have been found to be of lifesaving importance in the case of care for septic patients. Thorough understanding of the pathogens involved is therefore crucial. Using antimicrobial susceptibility testing, PCR and Multi Locus Sequence Typing (MLST), the molecular characteristics of ESBL producing E. coli isolates could be determined. This study can report that the most common ESBL producing genes found were CTX-M-14 (51 isolates, 45,5%), CTX-M-55 (23 isolates, 20,5%) CTX-M-15 (22 isolates, 19,6%). In addition, 2 isolates (1,8%) were found to be SHV-11 positive which is another ESBL producing gene. As a side finding, 5 isolates harbored Metallo-beta-lactamase (MBL) encoding genes such as NDM-5 and NDM-1 which were found to coexist with CTX-M-55 and CTX-M-14 respectively. An MLST analysis resulted in the finding of 25 different and 17 previously unknown (16,2 %) sequence types. The most common sequence types were ST131 (18 isolates, 17,1 %) as reported previously.  No significant differences in antimicrobial susceptibility were identified whether ESBL producing genes such as SHV and CTX-M was present or not. This study indicates that there could be novel resistance mechanisms present among those isolates not encoding the genes of interest. However, this finding requires further research before it can be confirmed.

Nearly seven hundreds of students each year apply for informatics' studies at University of economics, Prague (UEP). At the beginning, each one must complete two courses related to programming - Introduction to Programming and Fundamentals of Software Engineering. Both courses produce a lot of outputs -- simpler home works or more complex semestral projects -- which must be assessed by the teachers. Especially assessment of early home works is laboring and routine which makes it perfect candidate for automation. The goal of this work is to suggest the best suitable way how to implement this automation at UEP. Since mass courses make troubles universities all around the world there is already a lot of existing solutions how to automate evaluation of programming courses. First two chapters summarize the effort to find the most interesting ones and present them to the Czech audience. Because existing solutions are really sophisticated but does not provide white box tests support needed at UEP the rest of this thesis is not about creating own new automatic assessment system but about creating a middleware which can handle the problem mentioned. The middleware called Duckapter makes usage of Java reflection API easier and is downloadable from project website http://code.google.com/p/duckapter. Using them, the teachers can provide white box tests inside the black box (unit) tests which are supported by the most of the current automatic assessment systems.

O Brasil é o maior exportador de carne de frango e o terceiro maior produtor desta carne. O consumo desta fonte de proteína tem aumentado bastante nos últimos anos, tendo passado de 23,2 Kg/habitante em 1995 para 35,5 Kg/habitante em 2005. O mercado internacional tem se tornado cada vez mais exigente com relação aos padrões microbiológicos destes produtos. Pela importância das aves para a economia brasileira e por Listeria monocytogenes apresentar alta taxa de mortalidade, além de ser facilmente encontrada em carne de aves, decidiu-se verificar a ocorrência deste patógeno em dois matadouros, um com evisceração automática (Planta A) e outro com evisceração manual (Planta M), e traçar as possíveis rotas da disseminação do microrganismo na linha de processamento. Do total de 851 amostras coletadas de produtos, das superfícies de contato e de não contato com o produto, das mãos dos manipuladores e da água utilizada durante o processo de abate, 423 amostras foram da Planta A e 428 da Planta M. O teste VIP® Listeria foi utilizado para a triagem das amostras, sendo que aquelas positivas foram submetidas à caracterização fenotípica (provas bioquímicas e ágar cromogênico). A identificação e a tipagem das cepas foi realizada por técnicas moleculares (BAX® System, multiplex-PCR 16S rRNA, multiplex-PCR, ribotipagem e PFGE). L. monocytogenes foi isolada de 20,1% das amostras da Planta A, sendo 61,6% pertencentes ao sorogrupo 4b, 4d ou 4e; 19,2% ao sorogrupo 1/2a ou 3a; 15,2% ao sorogrupo 1/2c ou 3c; e 4,0% ao sorogrupo 1/2b, 3b ou 7. Na Planta M, 16,4% das amostras foram positivas para L. monocytogenes, havendo predomínio do sorogrupo 1/2a ou 3a (72,9%), seguido do sorogrupo 4b, 4d ou 4e (27,1%). Baseado nos resultados dos testes para caracterização fenotípica e genotípica, verificou-se que L. monocytogenes presente no produto final apresentou características semelhantes àquelas presentes na planta, e não no animal. Apenas uma cepa foi isolada na zona suja da Planta A, piso da seção de depenagem, e todas as demais foram isoladas da zona limpa de ambas as plantas. / Brazil is the first exporter of chicken meat and the third producer of this kind of meat in the world. The consumption of this protein source in Brazil has been increasing, having passed from 23.2 Kg/inhabitant in 1995 to 35.5 Kg/inhabitant in 2005. The international market has become more demanding for safety of these products. Because of the importance of this food commodity to Brazilian economy and because of Listeria monocytogenes importance as a foodborne pathogen this study was conducted. The presence of the pathogen in two facilities, one with automatic evisceration (Plant A) and another with manual evisceration (Plant M), was evaluated to identify possible routes of microorganism dissemination in the processing line. From a total of 851 collected samples of products, food contact and non-food contact surfaces, workers\' hands and water used in the process, 423 samples were from Plant A and 428 from Plant M. VIP® Listeria was used for the samples screening, positive ones were plated and suspected characteristic colonies submitted to biochemical characterization. Selected strains were submitted to identification and typing by molecular techniques (BAX® System, multiplex-PCR 16S rRNA, multiplex-PCR, ribotyping and PFGE). L. monocytogenes was isolated in 20.1% of the samples from Plant A with 61.6% belonging to serogroup 4b, 4d or 4e; 19.2% to serogroup 1/2a or 3a; 15.2% to serogroup 1/2c or 3c; and 4.0% to serogroup 1/2b, 3b or 7. From Plant M 16.4% of the samples were positive for L. monocytogenes, with predominance of serogroup 1/2a or 3a (72.9%) followed by serogroup 4b, 4d or 4e (27.1%). Based on the results of phenotypic and genotypic characterization, it was verified that L. monocytogenes present in the final product had similar characteristics to those isolated in the plant, and not in the animals. Only one strain was isolated in the dirty zone of Plant A, on the floor of defeathering section, and all others were isolated in the clean zone of both plants.

Scotts Creek, in Santa Cruz County, Calif., supports the southernmost extant population of Coho Salmon (Oncorhynchus kisutch) in North America. In 1997, the California Department of Fish and Game (now Fish and Wildlife) conducted an extensive habitat typing survey of mainstem Scotts Creek, describing all habitat units from the top of the estuary to the limit of anadromy approximately 12 km upstream. I repeated this survey in 2013 to (1) assess changes in the quantity and quality of instream habitat, (2) compare the current condition to goals and standards established in the federal Central California Coast (CCC) Coho Salmon Recovery Plan, and (3) identify opportunities for possible future restoration. A comparison of the two surveys revealed an overall increase in mean canopy cover, mean bank vegetation, mean percentage instream cover, pool depth diversity, and percentage riffles since 1997, and decreases in mean residual pool depth, percentage flatwater, and number of primary pools. Overall, the percentage of the total mainstem classified as pool habitat did not change between the two survey periods. Results for individual habitat metrics were more variable when the stream was broken into discrete reaches delineated by major tributary junctions. Although a large woody debris (LWD) survey was not conducted as part of the 1997 survey, contrasting our results with data collected during intervening years indicated that instream LWD has become more abundant, primarily due to increases in hard-wood species (i.e., red alder [Alnus rubra] and California bay [Umbellularia californica]). When compared to habitat goals established in the federal CCC Coho Salmon Recovery Plan, Scotts Creek has adequate canopy cover and percentage pools, but is lacking in percentage riffles, instream cover, key pieces of LWD per100 m, and percentage primary pools.

Nike is a prominent company that engages in brand activism, a marketing approach wherein they brand themselves as socially progressive by adopting public stances on controversial social issues. Because Nike resides at the intersection of media and sports, both of which are institutions that gender plays an important role in, an exploration of how Nike constructs gender in its commercials may illuminate the authenticity of its brand activism and progressive politics. Through a content analysis of 131 commercials released by Nike in the past decade, I explore whether their reputation as a socially progressive company extends to genuinely diverse and inclusive portrayals of gender in their commercials, or if they instead continue to rely on gender stereotypes. My results indicate that Nike commercials continue to treat sports as a predominantly masculine realm in which male athletes compete, therefore marginalizing athletes who are female or who do not display traditional masculine qualities. I also find that the bulk of athletes portrayed by Nike are those that adhere to gender stereotypes, indicating that Nike chooses to reinforce hegemonic masculine ideals rather than being genuinely inclusive and progressive in regard to gender.

Magister Scientiae Dentium - MSc(Dent) / Bite mark evidence has been admitted into US courts since the 1870s. It quickly gained popularity after the conviction of W.E. Marx in 1974 for manslaughter using primarily bite mark evidence. However, since the development of DNA typing and testing in forensic science, the emergence of wrongful convictions has placed the validity of bite mark evidence admissibility into severe dispute. This mini-thesis is a condensation of the past ten years’ worth of literature on the latest researches regarding bite mark evidence. The theory of the uniqueness of the human dentition is analysed. The accurate reproducibility of bite mark on skin with regard to distortion is discussed. Some bite mark court cases, including wrongful convictions are explored. Inconsistent expert opinions and the lack of standards amongst practitioners are also examined. The aim of this study is to summarize the validity of bite mark evidence in the courts of law.

This case study looks at upper-secondary school students’ opinions about the use of digital and non-digital tools in the classroom. One of schools’ most important aims is to prepare students for a well-adapted citizenship. In a world of digitalisation, schools are also implementing more and more digital tools in teaching situations and many students in Sweden have access to a computer each at school. This paper investigates how students feel about note-taking using paper and pen or computer, what they feel is important for their futures and what preferences they have when it comes to the use of digital media in their lessons. A focus group interview (n=7) was carried out. From the themes found through the interview, a questionnaire was constructed and answered by 66 students. The results show that students are divided in their preferences for taking notes by hand or by computer. Students see the benefits and limitations of both methods. They generally find it easier and faster to type on the computer, but are concerned about the distractions that the computer offers. They feel that hand-writing is more time-consuming but that it is better for remembering the material. Overall, they find that hand-writing is better for learning (better now) but that being dexterous using the computer is important for their future (better in the future). It is important that teachers foster both skills so that students get a balanced education.

Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen that has spread into the community since the 90s. In general, the community-associated (CA- MRSA) strains are more virulent, but less resistant to antimicrobials than the hospital- acquired strains (HA-MRSA). Some lineages of MRSA such as sequence type 8 have been transmitted more successfully around the world than others and there are situations where a dominant lineage has been replaced by a new one. The factors that are crucial for the selection of dominant lineage are often not clear. As part of this thesis, a longitudinal study of MRSA epidemiology in the Motol University Hospital during the period of 2018-2020 was performed and the occurrence of MRSA clonal complexes was characterized. A multicenter study of the epidemiological situation of MRSA in Slovakia during the period of January - April 2020 was also performed concomitantly. Moreover, several isolates from dominant lineages were further characterized by their phenotype. In the Motol University Hospital study, it was found that the HA-MRSA of the clonal complex 5 dominates and is represented mainly by spa-type t586; a finding that concurs with recent studies from the Czech Republic. In Slovakia, similar to the Czech Republic, MRSA lineages from clonal...

Methicillin-resistant Staphylococcus aureus (MRSA) is a variant of the more common Methicillin-Susceptible Staphylococcus aureus (MSSA), an opportunistic pathogen a portion of the human population carries as normal bacterial flora. When an outbreak of MRSA occurs, it is often important to determine if and how these strains are related to each other. In this report two different types of epidemiological methods were combined (namely Multi-Locus Sequence Typing and staphylococcal protein A-typing), in order to reduce workload, costs and time. A panel of resistance and virulence markers was also added to gather as much information about the culture as possible in a single analysis. To test the viability of the method extracted DNA and heat-treated bacterial cultures of both MRSA and MSSA were amplified with a curated panel of primers. These products were later sequenced with Nanopore’s MinION using the Flongle flow-cell. The method showed promise and worked as intended regarding the staphylococcal protein A-typing and the panel of resistance and virulence markers. However, the Multi-Locus Sequence Typing did still require optimization in order to be used clinically. In summary the project can be viewed as a success since it succeeded in being more time, cost and work efficient than many of its predecessors, when the problems with the Multi-Locus Sequence Typing are solved.

Genetic typing of Klebsiella pneumoniae is used for epidemiological referencing. In the clinical setting it can be useful in outbreak investigations, understanding transmission and managing hospital infections. Multi-drug resistant bacteria exist and proliferate either due to natural selection of clonal lineages or the transfer of mobile genetic elements, sometimes in response to antibiotic-use selective pressure. Pulsed-field gel electrophoresis (PFGE) is highly discriminatory and the gold standard typing method for the characterisation of K. pneumoniae isolates. The aim of the study was to genetically characterise K. pneumoniae isolates by PFGE and multilocus sequence typing (MLST). One hundred unrepeated ESBL-producing K. pneumoniae isolates were collected from the National Health Laboratory Service (NHLS). The PFGE was performed on a Rotaphor VI system (Biometra, Germany). Clonal representatives were further characterised by MLST. All the strains were typeable by PFGE using XbaI, which discerned multiple pulsotypes and MLST identified 10 different STs including a novel sequence type, ST1632. The diverse pulsotypes of K. pneumoniae isolates are not suggestive of clonal spread of particular strains. The MLST results further confirmed the variability among isolates tested and elucidated several STs, some of which have been identified internationally and often associated with carbapenem-resistance. Data on K. pneumoniae STs is still limited in the South African clinical setting, although the close monitoring of resistance profiles and characterisation of isolates is imperative for outbreak analysis, identification of prominent STs in clinical settings as compared to international counterparts and surveillance of expanding resistance. / Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2015. / Medical Microbiology / MSc (Medical Microbiology) / Unrestricted