The objective of this study was to determine time budgets, blood urea content, total protein and body condition score as adaptive responses to seasonal dynamics by breeding Nguni cows and heifers reared on a sweetveld. Twenty four clinically healthy Nguni cows and heifers were selected for the study. The animals were grouped according to their parities namely: Parity 1 (n=5), Parity 2 (n=5), Parity 3-6 (old cows) (n=9) and in-calf heifers (n=5). Two separate experiments were conducted and running concurrently. In the first experiment time budgets (time spent walking, grazing, lying down, drinking water and browsing) of the cows and heifers were monitored for two consecutive days once a month, while in the second experiment blood samples were collected from the coccygeal vein once a month during weighing. Body condition scores were also determined during weighing times. Heifers maintained significantly (p0.05) of both blood urea nitrogen (BUN) (7.5±0.39 mmol/l) and TP (80.7±1.19 g/l) in November. All the animals had the least BUN levels in January (1.93±0.18 mmol/l) while the highest total protein (TP) (82.1±1.08 g/l) was recorded in February. All the animals showed distinct (p0.05) in most behavioural attributes in the cool-wet season. Heifers spent significantly (p0.05) body condition scores, amount of time spent browsing and drinking water. On the other hand, heifers (14.7±1.50%) and first parity cows (13.9±1.50%) traded-off most (p<0,05) of their time walking during the cool-dry season. Similarly heifers spent more time browsing (6.4±0.62%) forage plants (p<0.05) than all the cows during the cool-wet season. Grazing (r = 0.17) and standing (r = -0.18) were correlated (p<0.05) with all the behavioural attributes measured in this environment. It was concluded that seasonal dynamics in trade-off behaviour through time budgeting, maintenance of BUN and TP were necessary for the Nguni cows and heifers to maintain their body condition scores within a narrow range throughout the four seasons.

Department of Animal Science / BSCAGR

No description available.

Thesis ( (M.Sc. Agriculture (Animal Production)) -- University of Limpopo, 2019 / A lack of energy and water in livestock production limit the performance of the animal. Therefore, the objective of this study was to determine the effect of spineless cactus as a source of energy and water on feed intake, feed digestibility and milk production of Holstein cows. Cactus serves as fresh forage or stored as silage for later feeding in livestock. However, there is limited information about cactus nutrients, their proportions in different species and the variability of these proportions with season. A total of four multiparous lactating Holstein cows with an average of 650 ± 80kg live weight were used and assigned to four dietary treatments with four replicates. Each animal was used four times. A cross-over design was used. The four dietary treatments were 0%, 4%, 8% and 12% of spineless cactus meal inclusion levels. The study period was 21 days of adaptation period and 6 days of collection period. Higher digestibility and energy supply (P<0.05) were observed with spineless forage diets compared to the control diet. Inclusion of spineless in the diets improved (P<0.05) diet intake and milk production in Holstein cows. However, an improved performance in milk production was observed with spineless cactus meal inclusion levels of 4%, 8% and 12%. Amongst the diets, 0% inclusion level had lower dry matter intake of 22.7kg (P<0.05) compared to those which had spineless cactus meal. High dry matter intake resulted in higher (P<0.05) milk production of 22.7, 24.7, 23.4 and 23.8 litres for 0, 4, 8 and 12% spineless cactus meal inclusion levels, respectively. However, cows on 4% inclusion level had higher milk yield than the other diets. Milk quality (butter fat, protein, lactose, somatic cell count and milk nitrogen urea) of Holstein cows was not (P>0.05) affected by the level of spineless cactus meal inclusion in the diet. Dry matter intake, neutral detergent fibre intake, acid detergent fibre intake and metabolisble energy intake of the cows were optimized at different spineless cactus meal inclusion levels of 19.427% (r2 = 0.992), 16.375% (r2 = 0.974), 14.0% (r2 = 0.984) and 22.909% (r2 = 0.994), respectively. Spineless cactus meal inclusion had no effect (P>0.05) on ADF intake and crude protein intake. Apparent digestibility values (0.73, 0.76 and 0.76) were not significantly different at 4%, 8% and 12% of spineless cactus meal inclusion levels, respectively. v It is concluded that spineless cactus meal inclusion level in the diet affected (P<0.05) diet intake and milk production of the Holstein cows. However, these variables were optimized at different spineless cactus meal inclusion levels. It could be recommended that spineless cactus can fit perfectly in livestock rations with attention given to their low crude protein content. These opuntia species can be fed to livestock mainly cattle and sheep during any season but are needed the most during drought as supplements when forages are scarce or expensive to feed. It is, therefore, concluded that 8% of spineless cactus inclusion level can be used to maintain productivity of the animals. / National Research Foundation (NRF)

Two metabolism trials were conducted with 15 wethers surgically equipped with duodenal and ileal cannulas to study the absorption of P and certain other minerals from swine waste and broiler litter. The effect of source and level of P on rumen cellulytic bacterial populations was also determined. Animals were fed a low P basal diet until serum inorganic P averaged 5.5 mg/dl, then randomly assigned to the following diets: low P basal alone, or supplemented with swine waste, broiler litter, dicalcium phosphate, or soybean meal. Trials consisted of a 7-d preliminary period, a 7-d collection of urine and feces and a 6-d sampling of duodenal and ileal digesta, and feces. Animals fed the waste diets tended to absorb more P than those fed the conventional supplements. Calculated by difference, sheep absorbed more P from swine waste and broiler litter than from dicalcium phosphate and soybean meal (P< .1). Less Ca was absorbed from the waste diets than from the conventionally supplemented diets (P< .05). Expressed as g/d, there was no difference in Mg absorption between waste and conventional diets. Sheep fed waste tended to digest more dry matter and a higher percentage of ADF in the large intestine. Lignin was primarily digested in the large intestine of all sheep. More cellulytic bacteria were isolated from the rumen of sheep fed the supplemented diets (P< .05). Both P intake and P recycling appear to be important influences on cellulytic bacteria in the rumen. Both broiler litter and swine waste appear to be good sources of P and Mg for ruminants. / Ph. D.

The main hypothesis tested in this thesis was that poor animal production from saltbush pastures is due to the negative effects of high sodium chloride (NaCl) and potassium chloride (KCl) on the ruminal environment, and subsequent effects on microbial populations and products of rumen fermentation. This main hypothesis was tested in two experiments. In the first experiment (Chapter Four) the effects of saltbush and a formulated high-salt diet on the ruminal environment and microbial populations were measured over 24-hours following feeding. Feeding both the saltbush and high-salt diet increased the salinity of the rumen fluid, but the formulated high-salt diet caused a decrease in ruminal pH while the saltbush caused an increase. This resulted in differences in the composition of the ruminal microbial populations between the sheep fed different diets. In the second experiment (Chapter Five) the effects of saltbush and a formulated highsalt diet on rumen fermentation were measured. Sheep fed saltbush had inefficient rumen fermentation and this was only partially explained by the high salt content of the diet. Diets containing high levels of NaCl and KCl provided low levels of net energy to sheep, but sheep fed saltbush lost more energy as methane and faecal energy compared to sheep fed the formulated high-salt diet. Inefficient rumen fermentation could help to explain poor animal production from saltbush pastures. Energy supplements such as barley grain can improve the value of saltbush pastures as feed for sheep, but there is no information on how much supplement is required. A third experiment (Chapter Six) was designed to test the hypothesis that there would be an optimal amount of barley required to improve the efficiency of rumen fermentation in sheep fed saltbush. Barley and straw were combined in a pellet and substituted for saltbush at 0, 20, 40, 60, 80 and 100% of the maintenance ration. Feeding barley and straw improved the efficiency of rumen fermentation in sheep fed saltbush, with an optimal level of supplementation at 60% of the maintenance diet. This is likely to be lower (approximately 20% of maintenance) if barley is fed without straw.

A series of experiments were conducted to determine nutritional strategies to improve the efficiency of N utilization in dairy cows when feeding co-products including wheat-based (W-DDGS) and corn-wheat blend distillers grains with solubles (B-DDGS), and dried whey permeate (DWP). In Experiment 1, the objective was to determine the effects of replacing canola meal (CM) as the major protein source with W-DDGS on ruminal fermentation, microbial protein production, omasal nutrient flow, and animal performance. Cows were fed either a standard barley silage-based total mixed ration containing CM as the major protein supplement (0% W-DDGS, control) or diets formulated to contain 10, 15 and 20% W-DDGS (dry matter [DM] basis), with W-DDGS replacing primarily CM. Diets were isonitrogenous (18.9% crude protein [CP]). Inclusion of W-DDGS to the diet did not negatively affect ruminal fermentation, microbial protein production, and omasal nutrient flow. However, there was a 0.7- to 2.4-kg increase in DM intake, and a 1.2- to 1.8-kg increase in milk yield after the addition of W-DDGS in place of CM. In Experiment 2, the objective was to delineate the effects of including either W-DDGS or B-DDGS dried distillers grains with solubles as the major protein source in low or high CP diets fed to dairy cows on ruminal function, microbial protein synthesis, omasal nutrient flows, urea-N recycling, and milk production. The treatment factors were type of distillers co-product (W-DDGS vs. B-DDGS) and dietary CP content (15.2 vs. 17.3%; DM basis). The B-DDGS was produced from a mixture of 15% wheat and 85% corn grain. All diets were formulated to contain 10% W-DDGS or B-DDGS on a DM basis. Feeding up to 10% of dietary DM as B-DDGS or W-DDGS as the major source of protein did not have negative effects on metabolizable protein (MP) supply and milk production in dairy cows. However, reducing dietary CP content from 17.3 to 15.2% decreased milk production. This response was attributed to an insufficient supply of ruminally degradable protein (RDP) that suppressed microbial nonammonia N (NAN) synthesis in the rumen, thus decreasing intestinal MP supply. In Experiment 3, the objective was to determine the effects of replacing barley or corn starch with lactose (as DWP) in diets containing 10% W-DDGS on ruminal function, omasal nutrient flow, and lactation performance. The treatment factors were source of starch (barley vs. corn) and dietary inclusion level of DWP (0 vs. 6%; DM basis) as a partial replacement for starch. Diets were isonitrogenous (18% CP) and contained 3 or 8% total sugar. The starch content of the low sugar diet was 24% compared to 20% for the high sugar diet. Dry matter intake, and milk and milk component yields did not differ with diet. However, partially replacing dietary corn or barley starch with sugar up-regulated ruminal acetate and propionate absorption, and reduced ruminal NH3-N concentration, but had no effect on ruminal pH, microbial protein synthesis, omasal nutrient flow and production in dairy cows. In summary, data presented in this thesis indicate that W-DDGS and B-DDGS can be included as the major source of protein in dairy cow diets without compromising ruminal function, nutrient supply and milk production in dairy cows. Feeding medium to low CP diets, and partial replacement of starch with sugar in diets containing W-DDGS and B-DDGS can improve N utilization efficiency in dairy cows. Additionally, an upregulation of facilitated transport of acetate and propionate across epithelial cells possibly prevents the occurrence of ruminal acidosis when lactose partially replaces starch in cow diets.

No abstract available. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

This research reported here was carried out to examine alternatives to antibiotic growth promoters as a result of their being banned in the animal feed industry. Four experiments were conducted to evaluate the efficacy of non-medicated feed additives as replacements for antibiotic growth promoters in broiler feeds. The additives used were enzymes (a new thermo-tolerant powder enzyme called TXAP, phytase, lipase and a new phytase enzyme derived from E. coli called phyzyme XP), organic acid (Acid Pak), prebiotic (Bio-Mos®) and probiotic (All-Lac XCL). Mashed maize-soya based feeds were used in all the experiments, which were conducted in litter-floor pens. The first experiment was a dose-response trial. Broilers in eight replicate pens of 50 males and 50 females were fed unsupplemented feeds and five additional feeds containing increasing levels of TXAP, from 0.5 to 2.5 g/kg to 42 d. The second experiment used enzyme TXAP with two different enzymes (phytase and lipase), individually or in combination. Six replicate pens of 50 males and 50 females were fed either unsupplemented feeds or one of six additional feeds treated with TXAP, lipase, phytase , a combination of TXAP and lipase, a combination of TXAP and phytase or a combination of all the three enzymes . This trial continued for 42 d. In the third experiment three types of TXAP (Lot 1, 2 and 3) were used, with fixed levels of xylanase and amylase but varying levels of protease activities (4000, 2000 and 1000 U/kg for Lot 1, 2 and 3, respectively) in combination with phyzyme XP for 35 d. The fourth experiment used mannan-oligosaccharide (Bio-Mos®), organic acid (Acid pak 2x), probiotic (All Lac XCL 5x), individually or in combination and an antibiotic growth promoter (Zinc bacitracin) for 42 d. The chickens in this experiment were challenged with Clostridium perfringens (CP) at 21, 22 and 23 d to determine the efficacy of these additives for replacing antibiotics in hindering the effects of CP on the villus surface area. The dose-response trial did not show any significant improvement in broiler performance with any level of inclusion of enzyme TXAP. The results from this study showed some beneficial effects with the use of enzyme TXAP when fed alone and at a young age. Its use when combined with other enzymes and at later stages of growth needs further investigation. Feed additives in experiment 4 prevented the negative effects of CP as the treated chickens did not have lesions on their villus surfaces. The conditions under which these trials were conducted appeared to be such that little benefit was derived from the use of any of the feed additives used. It is possible that under less-hygienic conditions such as those in commercial operations greater benefits from these additives may be realised. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Two experiments were conducted for this thesis, to determine whether an animal should be fed to its genetic potential in spite of this not being achievable due to an on-farm constraint. The first experiment was designed to compare the response of pigs housed either individually or in groups to a range of feeds limiting in lysine between 40 and 85 kg live weight. Two hundred and eighty-eight entire male Large White x Landrace pigs were used. The experiment was divided into two growth periods, i.e. from 40 to 60 kg and from 60 to 85 kg. In each period, pigs were subjected to feed containing one of four dietary lysine concentrations. In Period 1, the lysine concentrations were 11.03 (L1); 9.54 (L2); 8.00 (L3) and 6.51 (L4) g/kg, while in Period 2 these were 7.82 (T1); 6.71 (T2); 5.55 (T3) and 4.40 (T4) g/kg. Pigs fed an L1, L2, L3 or L4 diet in Period 1 were fed a T1, T2, T3 and T4 diet in Period 2, respectively. Three buildings provided the following group sizes and floor-space allowances: House 1 contained eight pigs per pen at 1.94 m2/pig; House 2 contained four or eight pigs per pen at 1.72 or 0.86 m2/pig; and House 3 contained one pig per pen at 1.72 m2/pig. The individually-housed pigs were divided into three feeding levels, i.e. ad libitum, or pair-fed so that feed intakes would match those of ad libitum-fed pigs housed in groups of either 4 (restricted-4) or 8 (restricted-8) pigs per pen in House 2. For all group sizes, feed intake increased linearly as the dietary lysine content increased. However, this increase was significantly lower for 8, when compared with 1 and 4 pigs per pen. The linear increase in feed conversion efficiency with dietary lysine content was similar for all group sizes. However, at any dietary lysine concentration, pigs housed in groups of 8 had significantly higher efficiencies than the pigs housed individually or in groups of 4. Average daily gain increased linearly as lysine intake increased, this increase being the same for all group sizes. However, pigs in smaller groups grew significantly faster than those in larger group sizes for any lysine intake. Protein and lysine retention were unaffected by group size, increasing linearly as lysine intake increased. The efficiency of lysine utilisation (0.45) was not impaired by group size. The pair-fed pigs housed individually (restricted-4 and -8) consumed significantly less feed than the individually-housed pigs fed ad libitum, and this was reflected in their average daily gains, which increased linearly as lysine intake increased, but with the restricted-8 growing significantly slower than the ad libitum or restricted-4 pigs. In all three treatments feed conversion efficiency increased linearly with dietary lysine content, although the restricted-4 and -8 had significantly higher efficiencies than the ad libitum-fed pigs at any dietary lysine content. Protein and lysine retentions were unaffected by feeding level and increased significantly with lysine intake. However, at any lysine intake the restricted-8 pigs had a significantly lower efficiency of lysine utilisation than the ad libitum or restricted-4 pigs. The pigs with floor-space allowances of 0.86 and 1.94 m2/pig consumed significantly less and grew slower than the pigs with floor-space allowances of 1.72 m2/pig at any dietary lysine content. Feed conversion efficiency was unaffected by floor-space allowance and increased significantly with dietary lysine content. Similarly, protein and lysine retentions were unaffected by floor-space allowance and increased linearly as lysine intake increased. The efficiency of lysine utilisation (0.45) remained unaffected by floorspace allowance. It was concluded that when animals are socially stressed, feeding according to the requirement for maximum protein growth produces the best biological performance and carcass composition, with the corollary that, if profitability and biological efficiency is to be maximised, pigs housed in stressful conditions, or those whose future performance is predicted to be below potential because of external stressors, should not be given feed of an inferior quality. The second experiment was designed to determine the extent to which grouping or floorspace allowance would alter the nutrient content of feed chosen by pigs given a choice of two feeds differing in protein: energy ratio between 40 to 85 kg live weight. Three hundred and eighteen entire male Large White x Landrace pigs were used. Two buildings provided the following group sizes and floor-space allowances: House 1 contained nine and eighteen pigs per pen at 1.72 or 0.86 m2/pig; House 2 contained four, nine and fourteen pigs per pen at 1.72; 0.86 or 0.49 m2/pig. Animals were given simultaneous ad libitum access to a high (236 g protein/kg as fed) and a low crude protein feed (115 g protein/kg as fed) in two hardened plastic self-feeder bins placed side-by-side. A training period of six days was used prior to the start of the trial, during which the two feeds were alternated daily. The reduction in the proportion of high protein feed chosen over time was significantly higher for the groups of four and eight, in comparison to the groups of nine and eighteen, contrasting with the steady increase for the groups of fourteen pigs. Similarly, the significant increase for pigs with floor-space allowances of 0.49 m2/pig differed from the significant decrease for pigs with floor-space allowances of 0.86 and 1.72m2/pig. Pigs housed in larger group sizes and smaller floor-space allowances consumed significantly less and grew slower than pigs housed in smaller group sizes and larger floorspace allowances. However, the feed conversion efficiency remained unaffected by group size and floor-space allowance. The non-significant effect on protein retention with increasing group size contrasted with the significant increase associated with increasing floor-space allowance. The results of the two studies were compared to determine whether pigs chose differently depending on the degree of stress and the implication of this choice. Average daily gain was significantly reduced as the group size increased for pigs fed a fixed lysine content and choice-fed. However, this reduction was less severe with choice-feeding than when feeding a fixed lysine content. Increasing the group size significantly reduced the feed intake in pigs fed a fixed lysine content only. The efficiency of protein utilisation remained unaffected as the group size increased for the pigs fed a fixed lysine content. However, at any group size pigs fed lower lysine contents had higher efficiencies than pigs fed higher lysine contents. On the contrary, increasing the group size significantly increased the efficiency of protein utilisation in choice-fed pigs. The average daily gain and feed intake was significantly improved as the floor-space allowance increased but was similar for pigs fed a fixed lysine content and choice-fed. Although the efficiency of protein utilisation remained unaffected by increasing the floor-space allowance for the pigs fed a fixed lysine content and pair-fed, at any floor-space allowance pigs fed higher lysine contents had higher efficiencies than pigs fed lower lysine contents. The results indicate that providing socially stressed pigs a choice between an appropriate pair of feeds differing in protein: energy ratio, does not overcome the reduction in potential growth, but does result in performance similar to that of pigs fed a fixed lysine content. It was concluded that the social stress of grouping or floor-space allowance has no influence on the ability of the animal to select an appropriate dietary combination allowing the expression of potential growth within the constraint(s) of the production system. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.