Master of Science / Department of Diagnostic Medicine/Pathobiology / Roman Reddy R. Ganta / Ehrlichia and Anaplasma species are rickettsial organisms which infect a variety of mammalian species. The organisms are transmitted from ticks and are maintained in reservoir hosts. Several pathogens have been identified in recent years as the causative agents for emerging infections in people. One of the primary reservoir hosts for the pathogens is the white tailed deer. In this study, 147 deer blood samples and 37 ticks were evaluated for the prevalence of Ehrlichia/Anaplasma species by TaqMan-based real time amplification assay and DNA sequence analysis. One hundred and thirteen (74%) samples tested positive with the Ehrlichia/Anaplasma genera-specific probe. Further analysis of the samples with the probes specific for human ehrlichiosis agents, E. chaffeensis and E. ewingii identified 4 (2.7%) and 7 (4.7%) positives, respectively. Test positives from 24 randomly selected samples were further evaluated by sequence analysis targeting to a 450 bp segment of 16S rRNA gene. All 24 samples were confirmed as positive for the Ehrlichia GA isolate # 4 (GenBank #U27104.1). DNAs from 37 pools of ticks collected from the white tailed deer were also evaluated. The TaqMan-based real time PCR assay with Anaplasma/Ehrlichia common probe identified 29 (78%) tick pools as positives whereas E. chaffeensis- and E. ewingii-specific probes identified three (8%) and one (3%) positives, respectively. The PCR and sequence analysis of tick samples identified Gram-negative bacteria species which included one endosymbiont of Rickettsia species (one tick pool), one Alcaligenes faecalis strain (three tick pools), five different Pseudomonas species (9 tick pools) and five different uncultured bacteria organisms (7 tick pools). Although the pathogenic potential of the white-tailed deer isolates of Anaplasma and Ehrlichia agents remains to be established, their high prevalence and the presence of human ehrlichiosis pathogens in white-tailed deer is similar to earlier findings. The high prevalence of the deer isolates of Anaplasma and Ehrlichia species demonstrates the need for further assessment of the pathogenic potential of these organisms to people and domestic animals.

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The main objective of this study was to establish a universally functional evaluation process for environmental education (EE) materials that can increase appropriate educational program application and resultant efficacy among users of all skill levels and disciplines, specifically those wildlife-related. Additionally, this research investigated capability of an EE program to alter preconceived high school student attitudes and knowledge toward urban white-tailed deer (Odocoileus virginianus) across varying demographics. I evaluated systematically a pre-produced urban wildlife classroom program, Living with White-tailed Deer (LWWTD), and measured student understanding of associated deer issues pre- and post-program. Using a detailed framework based upon the Guidelines for Excellence outlined by North American Association for Environmental Education (NAAEE), I created an instrument to identify EE programs indicative of high merit. During the 2007-2009 school years, trained classroom educators (n = 72) from 13 states were recruited to participate with their students (n = 1,274) in the 3-5 day LWWTD program and asked to critically assess it using my evaluation instrument. Teachers also administered attitude and knowledge assessments to their students. Teacher opinion toward the program was found to be highly favorable ( = 3.4/4) and was confirmed by significant increases in student knowledge before and after the program (P < .001). Regional differences in teacher response were found, but did not affect student performance. Teachers indicated that the Guidelines for Excellence are a meaningful tool in developing evaluative measures. Weak program components such as applicability to differing cultures were isolated using the evaluation instrument while strong components such as instructional soundness were highlighted. Pre- and postprogram student responses were correlated to demographic variables and differed significantly among races, gender, and urban or rural residency. Student experiences revealed also differences in attitude and knowledge of varying constructs relating to urban deer issues. An increase in knowledge following the LWWTD program was found across all demographic and experience variables suggesting high effectiveness regarding learning. Student attitudes following the LWWTD program showed an increased acceptability of lethal deer management techniques regardless of demographics, experience, or pre-program beliefs. These results suggest that effective EE can transcend predetermined beliefs.

I analyzed precision and accuracy of lactation data to reflect actual fawn recruitment for various levels of fecundity, neonatal mortality, harvest intensity, and hunter selectivity. I evaluated and developed a correction equation to adjust for harvest date effects on lactation detectability. I compared metrics of site-specific fawn recruitment (e.g., lactation rates, hunter observation fawn-to-doe ratios, and post-season fawn-to-doe ratios) among themselves, and to site-specific indices of carnivore abundance, for 18 properties across Mississippi and Alabama. Accuracy of lactation rates are compromised due to variation at minimal harvest intensity and insensitivity to change in fetal rate or neonatal mortality. Lactation data can be corrected for harvest date effects on lactation detectability. Hunter observation data are variable and poorly represent fawn recruitment. Lactation data are related to fawn recruitment and can be used to roughly estimate fawn recruitment. Carnivore abundance had neither a significant nor consistent effect on fawn recruitment.

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<p>White-tailed deer (Odocoileus virginianus; hereafter deer) are a keystone herbivore within forest ecosystems. While deer rely on plant species for growth, reproduction, and survival, multiple external factors can dictate browsing behavior. These factors ultimately drive browsing selection, browsing intensity, and diet composition, which in turn can shape the influence deer have on forest ecosystems. To better understand the complex relationship between deer populations, their habitat, and public perception of deer, the Indiana Department of Natural Resources partnered with Purdue University to initiate the Integrated Deer Management Project (IDMP). As part of the IDMP, this dissertation evaluated the ecological condition of deer habitat to assess the influence deer have on woody and herbaceous plant species within Indiana forests. Our study aimed to i) rank woody species according to their selection by deer and evaluate how the ranking of individual species varies across the state (Chapter 2); ii) evaluate variables and spatial extents associated with differences in browsing intensity, and evaluate different indices used to assess differences in browsing intensity (Chapter 3); iii) quantify winter deer diet composition using DNA barcoding to evaluate how diets vary across a gradient of deer densities, browsing intensities, non-native plant densities, and landscape characteristics (Chapter 4); and iv) evaluate the interactive effects of deer, non-native plant species, and landscape characteristics on the herbaceous layer of forests, while further evaluating the spatial extent at which landscape characteristics are most strongly related to herbaceous-layer composition and diversity (Chapter 5). To do this, I sampled 152 woodlots over three years across three regions of Indiana, collecting data on the browsing selection of individual woody species, the browsing intensities on all woody species, and the composition of vegetation communities (Chapters 2, 3, and 5, respectively). To address diet composition (Chapter 4), we collected deer pellet groups to analyzed diet components. We ranked a total of 63 woody species regarding their browsing selection by deer. While most of these remained consistent from region to region, 16 varied greatly in selection, as deer often showed increased selection for a given species when it resided in an area that provided greater browsing opportunities. Browsing intensity was most associated with food availability, however, it was also influenced by deer density in the region with the lowest forest cover. The twig age index of browsing intensity showed promise as the most efficient and effective index for use in Indiana woodlots. Although diet composition did not differ across regions of Indiana, we found 16 that deer consumed several uncommon taxa when the greater landscape exhibited homogenous patch composition. Similarly, deer consumed different native taxa in forested landscapes with greater deer densities in comparison to agricultural landscapes with lower deer densities. Lastly, though browsing varied within and across regions landscape characteristic, and not deer, were the most influential suite of variables. Additionally, the spatial extent at which these variables exhibited their best fit varied depending on the dependent variable being evaluated and the region of analysis. Together, our results highlight that variables ranging from the woodlot to the landscape-scale influence browsing behavior. This showcases that deer respond to variables at varying scales when browsing and in general, browse more in areas that offer the greatest benefit, whether these areas offer greater food availability or quality, or offer lower risks associated with anthropogenic development. This suggests that when managing forests for deer both woodlot and landscape context should play a role in the decision process. Although differences in browsing were observed, deer had less impact on the herbaceous layer compared to other variables we examined. This suggests that, in contemporary forests, landscape characteristics may be the drivers of changes, and species composition may reflect a long-term history of deer herbivory with less variability resulting from differences in contemporary deer abundance within and across regions.  </p>

Knowledge of the impacts of white-tailed deer (Odocoileus virginianus; hereafter deer) as dominant herbivores throughout the Southeastern United States of America is lacking. To address this, three paired experimental units of exclosures and controls were constructed in 2000 on three Wildlife Management Areas across Mississippi within the ecoregions of the Upper Coastal Plain, Lower Coastal Plain, and the Mississippi Alluvial Valley. Vegetation was sampled in the summers of 2000, 2005, and 2021 including vegetation structure, canopy coverage, basal area, and species composition. Additionally, in 2005 and 2021, biomass was sampled to determine potential impacts on nutritional carrying capacity. Among the three study sites, vegetation metrics followed successional trends and were not influenced by herbivory or lack thereof. Additionally, regional differences in nutritional carrying capacity seemed to be of greater importance than herbivory. This research illustrates the difference in effects of deer by region and forest type.

Prescribed fire is commonly used to manage white-tailed deer habitat. However, nutrition is still limited during the summer for deer in the Southeast and prescribed fire is commonly restricted to the dormant season. Knowledge of fire phenology effects on summer nutrient availability is relatively unknown. Stump sprouts may also affect available nutrition, which could be important when managing for summer nutrition. To address this summer nutritional stress period, I examined impacts prescribed fire phenology and mechanical stump sprouting had on summer nutritional carrying capacity for deer. Results indicated implementing prescribed fire in both dormant and growing-seasons led to increased summer nutrient availability on the landscape level by increasing forage quality and quantity. Mechanically creating stump sprouts from woody plants led to increased available nutrition on a localized level. Combining these management actions to target summer nutritional stress periods can better help deer meet nutritional demands and reach their full potential.

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