In dieser Arbeit wurden verschiedene Ansätze verfolgt, um das Verständnis des Saccharose-zu-Stärke Stoffwechselweges in sich entwickelnden Kartoffelknollen zu untersuchen. Zunächst wurde ein induzierbares Genexpressions-System aus dem Schimmelpilz Aspergillus nidulans für die Untersuchung des Metabolismus von Kartoffelknollen optimiert. Es wurde herausgefunden, dass dieses sogenannte alc system schneller auf Acetaldehyd reagiert als auf Ethanol, und dass Acetaldehyd weniger Seiteneffekte auf den Metabolismus hat. Die optimalen Induktionsbedingungen wurden dann benutzt um die Effekte einer zeitlich kontrollierten zytosolischen Expression einer Hefe-Invertase auf den Metabolismus der Kartoffelknolle zu untersuchen. Die beobachteten Unterschiede zwischen induzierter und konstitutiver Expression der Invertase führten zu der Feststellung, dass die Glycolyse erst induziert wird nachdem ein ATP-Mangel durch erhöhtes Saccharose-Cycling kreiert wurde. Weiterhin lassen die Ergebnisse darauf schließen, dass Maltose in der Kartoffelknolle eher ein Produkt der Kondensation zweier Glucose-Einheiten ist statt ein Produkt des Stärke-Abbaus zu sein. Im zweiten Teil dieser Arbeit wurde gezeigt, dass die Expression einer Hefe-Invertase in der Vakuole von Kartoffelknollen ähnliche Effekte auf deren Metabolismus hat wie die Expression des gleichen Enzymes im Apoplasten. Diese Beobachtung ist ein weiterer Beleg für die Präsenz eines Mechanismus, bei dem Saccharose mittels Endozytose in die Vakuole aufgenommen wird anstatt über Transporter direkt ins Zytosol aufgenommen zu werden. Zum Schluß wird ein kinetisches Modell des Saccharose-Abbaus vorgestellt, das in der Lage ist diesen Teil des Stoffwechsels der Kartoffelknolle quantitativ zu simulieren. Weiterhin kann dieses Modell die metabolischen Effekte der Einführung einer Hefe-Invertase in das Zytosol von Kartoffelknollen mit erstaunlicher Präzision vorhersagen. Zusammengefasst zeigen die Ergebnisse dieser Arbeit, dass induzierbare Genexpression sowie Computermodelle von Stoffwechselwegen nützliche Hilfsmittel für eine Verbesserung des Verständnisses des Pflanzenmetabolismus sind. / In this work different approaches are undertaken to improve the understanding of the sucrose-to-starch pathway in developing potato tubers. At first an inducible gene expression system from fungal origin is optimised for the use of studying metabolism in the potato tuber. It is found that the alc system from Aspergillus nidulans responds more rapidly to acetaldehyde than ethanol, and that acetaldehyde has less side-effects on metabolism. The optimal induction conditions then are used to study the effects of temporally controlled cytosolic expression of a yeast invertase on metabolism of potato tubers. The observed differences between induced and constitutive expression of the invertase lead to the conclusion that glycolysis is induced after an ATP demand has been created by an increase in sucrose cycling. Furthermore, the data suggest that in the potato tuber maltose is a product of glucose condensation rather than starch degradation. In the second part of the work it is shown that the expression of a yeast invertase in the vacuole of potato tubers has similar effects on metabolism than the expression of the same enzyme in the apoplast. These observations give further evidence to the presence of a mechanism by which sucrose is taken up via endocytosis to the vacuole rather than via transporters directly to the cytosol. Finally, a kinetic in silico model of sucrose breakdown is presented that is able to simulate this part of potato tuber metabolism on a quantitative level. Furthermore, it can predict the metabolic effects of the introduction of a yeast invertase in the cytosol of potato tubers with an astonishing precision. In summary, these data prove that inducible gene expression and kinetic computer models of metabolic pathways are useful tools to greatly improve the understanding of plant metabolism.

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By quoting Deuteronomy 30:12–14 as the content of the message of the righteousness of faith over against Leviticus 18:5 and the righteousness of the law in Romans 10:5–8, Paul proclaims a promise fulfilled in accord with the original meaning of the text written by Moses in Deuteronomy. More precisely, Paul reads Deuteronomy 30:11–14 as an extension of the reality foretold in Deuteronomy 30:1–10, which points forward to the new covenant experience of faith-empowered obedience, or heart circumcision, which includes the internalization of the word of God—the eschatological torah—by the Spirit of God. What Paul has found in Deuteronomy 30:11–14 is a prophetic promise of righteousness which he declares fulfilled in the gospel of the Lord Jesus, the message of the righteousness of faith.

The diploma thesis "Athletes as heroes - construction and evolution of Czech olympic medalist image" deals with media construction of olympic Winter image and its following trasformation into national hero myth. It approaches the topic from the perspective of semiotic analysis. It informs about the key concepts of semiotics and media studies like visual communication, sign, code, myth, social construction of reality, representation and stereotype. These concepts are consequently used as tools for the analysis of photos (thein legends) and headlines in daily press. The text shows on the case of six olympic winners (between years 1998 - 2008) how Czech daily press dealt and built individual stories a basic archetypes. In its first part it offers basic theoretical frame serving to understand the hero image as the social construct. Athlete with his win attracts new - medial constructed - identity. In the second part text tries to analyse media contents that deal with athetes images immediate after their olympic victory. It arrives to following conclusions: media have a tendency to produce the stories od victory, that are gender unequal and stereotyped. And finally, text show us individual images, their basic signs and confrontation, that signifies very similar narrative pracices and zero progress in time.

Die vorliegende Arbeit beschäftigt sich mit der Synthese und den Eigenschaften von linearen und verzweigten amphiphilen Polypeptid-Blockcopolymeren. Die Frage nach dem Einfluss der Topologie und Konformation der Blockcopolymere auf die supramolekularen und kolloidalen Eigenschaften bildete einen wichtigen Aspekt bei den Untersuchungen. Die Blockcopolymere wurden nach einem mehrstufigen Reaktionsschema durch Kombination von anionischer und ringöffnender Polymerisation von Aminosäuren-N-Carboxyanhydriden (NCA) synthetisiert. Die Untersuchung der Polypeptid-Blockcopolymere hinsichtlich ihres Aggregationsverhaltens in fester Phase sowie in verdünnter wässriger Lösung erfolgte mittels Streumethoden (SAXS, WAXS, DLS) sowie abbildender Methoden (TEM). Durch Einsatz der Blockcopolymere als polymere Stabilisatoren in der Emulsionspolymerisation wurden Oberflächen funktionalisierte Latizes erhalten. Als Beispiel für eine pharmazeutische Anwendung wurden bioverträgliche Polypeptid-Blockcopolymere als Wirkstoff-Trägersysteme in der Krebstherapie eingesetzt. / This work describes the synthesis and characterization of linear and branched polypeptide block copolymers having amphiphilic character. The studies focused on the impact of the block copolymers' conformation and architecture on the supramolecular and colloidal properties. The polypeptide block copolymers were prepared in a multi-step process involving the anionic synthesis of (poly)amino-functional polymers (polystyrene and polybutadiene) which where used as macroinitiators for the ring-opening polymerization of N-carboxyanhydrides (NCA) of protected a-aminoacids. Supramolecular structures of the block copolymers in the solid state as well as in diluted aqueous solution were investigated using scattering (SAXS, WAXS, DLS) and microscopic (TEM) methods. Both linear and branched polypeptide block copolymers were used as polymeric stabilizers in the emulsion polymerization of styrene to yield polypeptide-decorated latexes. Biocompatible block copolymers were used as drug-delivery systems in lymphatic cancer therapy.

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Estate planning is the arrangement and management of an estate owner’s estate to the effect that the estate owner and his beneficiaries can enjoy maximum benefit from his worldly possessions during his lifetime and after his death. Unfortunately, for estate owners and their beneficiaries, a deceased estate has to pay an executor’s fee, estate duty and capital gains tax on the demise of the estate owner, which means the amount the estate owner intended his heirs to receive, might be substantially decreased. For decades trusts have been used for estate planning purposes. The decision of the estate owner to utilise a trust for estate planning purposes involves the disposal of growth assets from the estate owner’s estate to the trust. This ensures that the value of a growth asset is pegged in the estate owner’s estate and the asset continues to grow in the trust. The asset is disposed of by way of a loan account in favour of the estate owner and the parties agree that the outstanding amount is payable on demand. In his will, the estate owner then bequeaths the outstanding amount back to the trust. However, Paragraph 12(5) of the Eighth Schedule to the Income Tax Act 58 of 1962 stated that capital gains tax will be levied in cases where a debt is reduced or discharged by a creditor for no consideration or for an amount which is less than the outstanding amount. In ABC Family Trust ITC 1793 the estate owner transfer assets to the trust on a loan account and in her will, bequeathed the exact outstanding amount back to the trust. It was argued on behalf of the trust that the bequest constituted a set-off and not a discharge of the debt. However, the court stated that the set-off took place because of the “operation of law” which is specifically included in the definition of a “disposal” for capital gains tax purposes. The court applied Paragraph 12(5) and found that the trust is liable to pay capital gains tax on the full outstanding amount. In XXX Trust ITC 1835 the estate owner also transferred asset to a trust, but in her will she bequeathed the residue of her estate, and not the exact outstanding amount, to the trust. In this case the court placed emphasise on the intention of the estate owner and not on the possible application of Paragraph 12(5). The court found that it was not the intention of the estate owner to discharge or reduce the debt for no consideration. Subsequently, it was found that the trust is not liable for capital gains tax. Since these two cases Paragraph 12(5) has been deleted and Paragraph 12A inserted in the Eight Schedule to the Income Tax Act 58 of 1962. The focus of this mini-dissertation is to determine which estate planning tools were available to estate owners to prevent a capital gains liability under Paragraph 12(5). The capital gains tax effect that Paragraph 12A might have on estate planning is also discussed. / LLM (Estate Law), North-West University, Potchefstroom Campus, 2014

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