Kohelová E: Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. II Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017, 77p. The aim of the diploma thesis was a preparation of alkaloidal extract from fresh bulbs of Narcissus cv. PROFESSOR EINSTEIN to izolate two Amaryllidaceae alkaloids by column and thin layer chromatography. Subsequently these alkaloids were subjected to structural MS and NMR analysis and tested for biological activity against human cholinesterases (HuAChE and HuBuChE) and for cytotoxic activity. In cooperation with the Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, antifungal and antimicrobial activities of alkaloids were tested, and in cooperation with Faculdade de Medicina da Universidade de Lisboa Portugal antimalarial activity of alkaloids was studied. Isolated substances were identified as galanthamine, caranine and pluviine. Pluviine was isolated in an amount allowing only structural analysis. In the test for determining cholinesterase inhibitory activity of caranine the following values were obtained: IC50, HuAChE = 320 ± 42 μM a IC50, HuBuChE = 486 ± 56 μM. Galanthamine (IC50,...

Marečková, L .: Biological activity of secondary plant metabolites VII. Alkaloids of Vinca minor L. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Kralove, Hradec Králové, Department of pharmaceutical botany and ecology, Hradec Králové 2018 There was prepared an extract from aerial parts of the plant Vinca minor L. This prepared extract was separated by the column chromatography into individual fractions. One chosen fraction was separated by TLC on silicagel. Two substances were isolated and they we identified by GC/MS and NMR as (-)-minovin and (+)-minovincin. These two substances were tested for their inhibition activity against human acetylcholinesterase (hemolysate of human erythrocytes) and butyrylcholinesterase (human plasma). Their inhibition activity was compared to standards which were Galanthamin, Huperzin A and Eserin. (+)-Minovincin did not show significant inhibitory activity against either enzyme (IC50 = AchE > 1000 µM, BuChE 655,05 ± 35,37 µM) and it is not suitable for further investigation. (-)-Minovin showed no significant activity against AChE (IC50 = 234,27 ± 63,40 µM), activity against BuChE (IC50 = 26,32 ± 2,52 µM) was higher than inhibitory akctivity of Galanthamin, but still relatively low for further investigation. The aim of this work was mainly the...

Schickerová Julie: Alkaloids of Narcissus pseudonarcissus cv. Dutch Master: isolation, structural identification, preparation of analogues, biological activity. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2018, 73 p. The purpose of this diploma thesis was to isolate the substances from the fraction ND 15- 9, which was obtained by column chromatography of the alkaloid extract of Narcissus pseudonarcissus cv. Dutch Master. The method of preparative TLC was used to separate this fraction, and the three purified compounds were isolated in the pure state NDS1-NDS3. NMR, GC/MS and optical rotation were used to determine their structure. The obtained data were compared with data in the literature and further studies on their biological activity were performed. Isolated substances were identified as epimaritidine, crinine and tetrahydromasonine. Their inhibitory activities (IC50, AChE > 1000 μM, IC50, BuChE > 1000 μM) versus human erythrocyte AChE and plasma BuChE were inactive compared to galanthamine standards (IC50, AChE = 1.71 ± 0.07 μM, IC50, BuChE = 42.30 ± 0.10 μM), huperzin A standards (IC50, AChE = 0.033 ± 0.001 μM, IC50, BuChE > 1000 μM) and berberin standards (IC50, AChE = 0.71 ± 0.01 μM, IC50, BuChE = 30.7 ± 3.5...

Klíčová A.: Alkaloids from wood of the species Liriodendron tulipifera L. and their biological activity. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department: Pharmaceutical Botany, Hradec Králové, 2018. Supervisor: PharmDr. Anna Hošťálková, Ph.D. Key words: Liriodendron tulipifera, secondary metabolites, alkaloids, biological activity Alzheimer's disease (AD) is the most common type of dementia. It is neurodegenerative disorder. Patients suffer from cognitive disorder in combination with aphasia, apraxia or disorders of executive functions, which leads to exacerbation of working or social skills. Nowadays, there exist no casual treatment for AD, and that is why the intense research is still waging. Currently there are available remedies, which can slow down progress of AD. These drugs includes also natural substances. An alkaloid extract of Liriodendron tulipifera L. wood showed promising inhibition of human cholinesterases in a preliminary testing. These results were the reason why this extract has been chosen for further isolation of single alkaloids and identification of their biological activity. Isolation was made by column chromatography with step gradient elution. Then was used preparative TLC to isolate single alkaloids. Identification of alkaloids was...

The hypothesis that experimental manipulations increasing life span correlate to reduced physical activity and reduced molecular oxidative damage was tested. We used three longevity phenotypes of males and females of Pyrrhocoris apterus, diapause insects, reproductive insects and insect with ablation of the corpus allatum, an endocrine gland producing juvenile hormone. Protein carbonyl content in thoracic muscles was used as an index of molecular oxidative modification.

At present we are witnessing a revolutionary period marked by changes in population demographics. Recently there has been a significant increase in life expectancy of people in developed countries and the percentage of seniors in society has been rising. The issue of a proactive approach to aging is therefore very relevant today. It is difficult to determine when youth ends and old age starts, but the World Health Organization has determined this age limit as 60 years. This is the time when people retire and when they perceive their own aging most intensely. The aim of the thesis is to explore the lifestyle of seniors. The theoretical part includes a description of this particular period of human life, the practical research studies the lives of concrete seniors with the aim of creating a clear conception of how today's seniors live, what their normal days look like, what they are occupied with and what they are worried about. The main research groups are couples, but in the research are included also individuals, mostly widows who live alone. Interviews conducted in their home environment enable looking into their privacy, and finding out what the lifestyle of people over 60 years old is like. For all respondents, retirement was a major turning point in their lives. The way they coped with this situation is different in each case, but anyway their lives and lifestyles changed in some way, either positively or negatively. Some people slowed down the pace of life and began to pursue hobbies and family life after leaving their jobs, others used the newly gained time to start to fully focus on their hobbies. There are, however, people who gave up their hobbies and got indolent. This applies particularly to lonely people. The research has shown that people living in pair are more active and if they are not limited by health problems, they show greater diligence and activity. Partners support each other in their hobbies and encourage each other to action. The research could not cover the views of all seniors, but it has turned out that a positive approach to life and an active lifestyle significantly helps improve old age that should not be seen as a sad and final chapter of life.

The thesis contains two parts. The teoretici part deals with the topic of free time and a healthy way of spending it. It also describes the udukational activities in youth hostels, the profession of an educator and it shows some possibilities how educators can influence spending the free time of the teenagers living in youth hostels. The main task of the empirici part is the description of educative effect on a healthy way of spending the free time of teenagers who live in youth hostels is focused on a helthy way of spending the free time.

The Diploma Thesis is focused on movement activity of students of Physical Training of Pedagogical Faculty of the University of South Bohemia. The research was conducted in the spring 2009 using the students of Physical Training aged 19 ? 26. Subsequently, the research was compared with the research conducted in the spring 2007 using the students of Physical Training. Data and information concerning the movement activity were obtained from two individual sources, the pedometer SW ? 700 and the latest version of NQLS questionnaire.

This dissertation is focused on three species of bdelloid rotifers Habrotrocha thienemanni, Macrotrachela insolita and Philodina roseola. We compared filtration activity, clearance rate and food selectivity within species, populations of the same species and clones.

Instead of using telomerase, Drosophila melanogaster extends its telomeres by transposition of special telomeric retroelements (HeT-A, TART and TAHRE) targeted specifically to chromosome ends. One key step of the transposition mechanism is a transcription of the elements. Using the expression of a reporter Tomato transgene under HeT-A promoter control we obtained a spatial and temporal visualization of HeT-A promoter activity during the whole Drosophila development. This analysis confirmed that the activity of the HeT-A promoter is up-regulated by cell proliferation, however HeT-A promoter activity is not limited only to proliferating diploid cells. One important outcome of this study is the observation of variation in HeT-A promoter activity in both location and intensity.