Made available in DSpace on 2014-06-11T19:23:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-13Bitstream added on 2014-06-13T19:50:24Z : No. of bitstreams: 1 botaro_ja_me_arafcf.pdf: 1079828 bytes, checksum: e3d6aa320e7262e913df25c024a8740c (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Recentemente, a indústria de alimentos está preocupada em desenvolver novos produtos, usando ingredientes inovadores, que promovam benefícios à saúde. O uso de proteínas de leguminosas como uma alternativa para a proteína animal tem sido estudada. O tremoço branco (Lupinus albus L.) apresenta elevada concentração de proteína e óleo, principalmente ácidos graxos insaturados e poliinsaturados, fibra alimentar, açúcares e vitaminas B1 e B2. Por outro lado, o tremoço não pode ser consumido diretamente porque possui alcaloides, que além de serem tóxicos, atribuem gosto amargo ao grão. Como a maioria dos alcaloides é solúvel em água, a quantidade de alcaloide do grão pode ser diminuída através de água corrente, fervente ou salmoura. Sucos de frutas são bastante consumidos no Brasil, especialmente no verão, não somente pelo sabor, mas como fonte de hidratação e por serem ricos em nutrientes. Os sucos de frutas tropicais conquistam cada vez mais o mercado consumidor, sendo o Brasil, um dos principais produtores. A pitanga, fruto da pitangueira (Eugenia uniflora L.) é uma planta frutífera nativa do Brasil (das regiões Sul e Sudeste e que tem se adaptado favoravelmente às condições climáticas e edáficas da região Nordeste), da Argentina e do Uruguai. A pitanga é uma fruta cuja polpa apresenta excelentes condições para industrialização, devido ao seu alto rendimento, aroma agradável e sabor exótico. O objetivo do presente estudo foi o de verificar, através da metodologia de superfície de resposta, as melhores condições para a utilização simultânea de extrato aquoso de tremoço branco e de suco tropical de pitanga, no desenvolvimento de uma bebida. Para tanto, as variáveis independentes foram representadas pelo volume de extrato aquoso de tremoço (mL) e pelo volume de suco de pitanga... / Recently, the food industry is concerned about developing new products, using innovative ingredients that promote health benefits. The use of vegetable protein as an alternative to animal protein is being studied. The white lupine (Lupinus albus L.) has high concentrations of protein and oil, especially non saturated fatty acids and polynonsaturated fats, dietary fiber, sugars and vitamins B1 and B2. On the other hand, the lupine bean can’t be consumed straight because it has alkaloids which besides being toxic, add a bitter taste to the bean. As most alkaloids are water soluble, the amount of alkaloid in the beans can be reduced by running water, boiling or brine. Fruit juices are widely consumed in Brazil, especially in the summer, not only for their flavor, but as a source of hydration and because they are rich in nutrients. The tropical fruit juices are conquering the market more and more, with Brazil being one of the main producers in the world. The Pitanga (Eugenia uniflora L.) is the fruit of the Pitangueira, a native plant of southern Brazil (which has adapted positively to weather and soil conditions in the country’s Northeast), Argentina and Uruguay. Pitanga is a fruit which pulp presents excellent conditions for industrialization, due to its high yield, pleasant aroma and exotic flavor. The aim of the present study was to verify, through the response surface methodology, the optimum conditions for the combined use of aqueous extract of white lupine and tropical pitanga juice in the development of a drink. Therefore, the independent variables were represented by the volume of aqueous extract of lupine (mL) and by the volume of pitanga juice (mL). The dependent variables (responses) were obtained through sensory test of acceptance (appearance, aroma, taste and overall acceptability). The combined... (Complete abstract click electronic access below)

La incorporación de leguminosas en los agroecosistemas ganaderos, presenta una serie de ventajas productivas y ambientales, respecto a los sistemas basados únicamente en gramíneas. El objetivo del trabajo fue analizar el efecto de la incorporación de leguminosas en pasturas puras de agropiro alargado (Thinopyrum ponticum) en la región semiárida. Para ello, se condujeron una serie de experimentos con el objetivo de evaluar específicamente (1) el efecto de la intersiembra con vicia (Vicia villosa) sobre la biomasa forrajera, valor nutricional de la pastura y dinámica de las fracciones orgánicas del suelo en tres ambientes del sudoeste bonaerense (Bahía Blanca, Tornquist y Cabildo), (2) el efecto de la densidad de intersiembra con vicia o trébol de olor blanco (Melilotus albus), sobre los cambios de valor nutricional en función de la composición botánica, y por último, (3) determinar el momento de corte óptimo para la confección de reservas (heno o ensilaje) y estudiar el efecto de la incorporación de grano de cereal en ensilaje de pasturas consociadas. Las mezclas forrajeras se realizaron intersembrando vicia o trébol en otoño de cada año, sobre agropiro implantado. El forraje se cortó manualmente, sobre una superficie de 0,4 m2 y la frecuencia de defoliación estuvo basada en el crecimiento de agropiro. Durante un período de dos años sobre una pastura monofítica de agropiro y una consociación con vicia (20 kg ha-1), se evaluó la biomasa forrajera y su valor nutricional (FDN, FDA, LDA, DIVMS y PB). Durante este ensayo se realizó un fraccionamiento físico del suelo, para separar las diferentes fracciones que componen la materia orgánica hasta la profundidad de 0-20 cm. En otro ensayo, se decidió estudiar el efecto de las densidades de intersiembra, se incluyeron en este caso dos especies de leguminosas (vicia o trébol). Ambas, se intersembraron separadamente a tres densidades diferentes, en función de plantas agropiro:leguminosa (80:20, 70:30 y 60:40). Por su parte, para el estudio de reservas forrajeras, en otro ensayo, se cortó una pastura pura de agropiro en cinco estados fenológicos, desde vegetativo (7/11/2012) hasta grano pastoso (14/02/2013) para la elaboración de heno y ensilaje en cada fecha. Con esta información, en un último ensayo se estudió el efecto de la inclusión de una leguminosa (trébol) y grano de avena en ensilajes de agropiro. Las mezclas de forraje y grano se realizaron en laboratorio combinando tres proporciones de trébol (T0: sin trébol, agropiro puro; T20: 20% trébol y T40: 40% trébol) y dos niveles de grano (G0: sin grano y G1: 30% de grano) en base a la MS. Cuando se intersiembra vicia o trébol sobre agropiro, se registró en todos los sitios un aumento en la producción forrajera anual que fue del 28 a 190%, salvo para la localidad de Cabildo que la producción no cambió (p=0,08). Analizando la producción de agropiro separadamente no se observa una disminución con la inclusión de vicia. Por lo tanto, se entiende que la leguminosa acompañante, explora recursos que el agropiro no hace. En cambio, se encontró que trébol ejerció una competencia con agropiro avanzada la primavera, porque a medida que aumentó la biomasa de trébol disminuyó linealmente la biomasa de agropiro. Comparando la producción forrajera de vicia y trébol, ambas especies mostraron una buena capacidad de compensación en el crecimiento, ya que no mostraron diferencias en el rendimiento a diferentes densidades. En general, la intersiembra con leguminosas aumenta el contenido de PB (entre 1,0 y 1,4% cada 10% de leguminosa en la composición), DIVMS y LDA de la pastura, mientras que disminuyó los contenidos de FDN (1,5% cada 10% de leguminosa) y FDA. Además, se identificó trasferencia de nitrógeno de vicia y trébol hacia agropiro por mayor contenido de PB del agropiro consociado, tanto en primavera como en otoño. La inclusión de vicia incrementó el contenido de Carbono orgánico particulado (COP) grueso (hasta 42%) y fino (hasta 13%) del suelo, sin modificar el contenido de carbono orgánico total. Para Cabildo y Bahía Blanca la inclusión de vicia también generó un aumento (12%) del nitrógeno de la materia orgánica particulada. Respecto a la elaboración de reservas de agropiro, se encontró que el heno mantuvo mejor el valor nutricional que el ensilaje. Aunque, la mayor fuente de variación se relacionó al estado fenológico de la pastura en el momento de corte y no al tipo de reserva elaborada. Para la elaboración de un adecuado ensilaje de agropiro, el período óptimo de corte va desde espiga embuchada a antesis, mientras que para henificación el período óptimo es mayor, desde estado vegetativo hasta antesis. La incorporación de una leguminosa como el trébol y el grano de avena al ensilaje de agropiro generó aumentos en el contenido de PB y disminuciones en FDN, FDA. La adición de grano, mejora la DIVMS en los tratamientos con menor cantidad de trébol (T0 y T20). A medida que aumentó la participación de trébol, los valores de pH se incrementaron progresivamente de 3,78 (T0) a 4 (T40). Sin embargo, la adición de grano generó una disminución de pH. El ensilaje con trébol de olor blanco hasta el 40%, no presentó un problema para la realización de un adecuado ensilaje, resultando la mejor alternativa junto con la incorporación de grano. / The inclusion of legumes in livestock agroecosystems have production and environmental advantages, compared to systems based only on grasses. The objective of this work was to analyze the effect of the inclusion of legumes in pure pastures of tall wheatgrass (Thinopyrum ponticum). A series of experiments were conducted with the objective of evaluating, (1) the effect of interseeding hairy vetch (Vicia villosa) over the forage biomass, nutritional value of pasture and dynamics of soil organic matter fractions in three representative semiarid environments of the Buenos Aires province, Argentina (Bahía Blanca, Tornquist and Cabildo), (2) the effect of the density of interseeding with hairy vetch or white sweetclover (Melilotus albus) over nutritional value depending on the botanical composition, and finally, (3) determining the optimal cutting moment for the preparation of reserves and study the effect of the incorporation of grain in silage of mixture pastures. The forage mixtures were made by interseeding hairy vetch or clover in the autumn of each year, on implanted tall wheatgrass. The forage was cut manually, on a surface of 0,4 m2 and the frequency of defoliation was based on the growth of tall wheatgrass. During a two-year period, on tall wheatgrass monoculture and mixture with hairy vetch (20 kg ha-1), biomass yield and its nutritional value (NDF, ADF, ADL, IVDMD and CP) were evaluated. During this period, soil organic matter fractions were separated by size using a wet-sieving method up to the depth of 0-20 cm. Besides, to study the effect of interseeded densities, another test was realized that included two species of legumes (hairy vetch or white sweetclover). Separately, both legumes were interseeded at three different densities based on the tall wheatgrass:legume plant relationships of 80:20, 70:30 and 60:40. Finally, for the study of reserves, a pure pasture of tall wheatgrass was cut in five phenological stages from vegetative (11/7/2012) to dough-grain (2/14/2013) for the preparation of hay and silage on each date. With this information, in another trial the effect of the inclusion of a legume (clover) and grain of oats in silages of tall wheatgrass was studied. The mixtures of forage and grain were made in the laboratory combining three proportions of clover (T0: without clover, pure tall wheatgrass, T20: 20% clover and T40: 40% clover) and two grain levels (G0: no grain and G1: 30 % grain) based on the MS. When hairy vetch or clover was interseeded over tall wheatgrass, an increase in annual yield biomass was recorded from 28 to 190%, except in Cabildo, where production did not change. Analyzing tall wheatgrass biomass separately, there is no decrease in production with hairy vetch inclusion. Therefore, the accompanying legume, seems to use the resources that tall wheatgrass leaves available. Instead, the clover competed with tall wheatgrass in late spring, thus, as the biomass of clover increased, the biomass of tall wheatgrass decreased linearly. Hairy vetch and clover plants showed growth compensation because there was no difference in forage yield at different density. In general, the hairy vetch or clover interseeding increased total CP (between 1,0 - 1,4% every 10% of legume in the composition), IVDMD and ADL, while the NDF (1,5 % every 10% of legume in the composition) and ADF decreased. In addition, the nitrogen transfer from the legume appear to be responsible for the increase in the CP content of tall wheatgrass when growing together. This was evident in both seasons spring and fall. Hairy vetch increased the content of particulate organic carbon (POC) in fraction >105μm (up to 42%) and fraction between 53 and 105 μm (up to 13%) of the soil. The total organic carbon in soil (SOC) content did not change, therefore the relationship (POC/SOC) was increased. For Cabildo and Bahía Blanca hairy vetch inclusion enhanced the N content (12%) of the particulate organic matter. Regarding the preparation of wheatgrass reserves, it was found that hay maintained its nutritional value better than silage. Although, the greatest source of variation can be attributed to the phenological stage of the pasture and to a lesser extent to the type of reserve. For the best silage of tall wheatgrass, the optimal cutting period was from booting to anthesis, whereas, for haymaking the optimal period extends from vegetative to anthesis. Inclusion of clover and grain increased silage CP content and decreased NDF and ADF. In addition, grain incorporation improved IVDMD in treatments with less clover (T0 and T20). The highest proportion of clover in the mixture reduced FDN and FDA. As clover participation was greater, the silage pH values increased progressively from 3.78 (T0) to 4 (T40). However, the addition of grain decreased pH. According to these results, a silage including white sweetclover up to 40%, was not a restriction to obtain a fairly good quality silage, being the best alternative when also combined with grain.

In Karpfen sächsischer Karpfenteichwirtschaften spielen u.a. die Zestoden Neogryporhynchus cheilancristrotus und Paradilepis scolecina und der Trematode Diplostomum pseudospathaceum eine Rolle (KAPPE 2004, PIETROCK et al. 2006, KAPPE & SEIFERT unveröff.). Weitere Fischarten (Schleie, Plötze, Regenbogenforelle) sind mit den Trematoden Diplostomum sp., Hysteromorpha triloba, Posthodiplostomum cuticola infiziert (PIETROCK et al. 2006). Mit dieser Arbeit sollte der Versuch unternommen werden, die Bedeutung Fisch fressender Vögel als Vektor Fisch pathogener Helminthen in Sachsen aufzuklären. Dafür wurden im Zeitraum von Juni 2003 bis Dezember 2004 97 Kormorane (Phalacrocorax carbo) und 46 Graureiher (Ardea cinerea) aus dem Einzugsgebiet sächsischer Teichwirtschaften auf ihren Befall mit Endohelminthen untersucht. Im Winter 2004 konnte zusätzlich ein Silberreiher (Casmerodius albus) und eine Silbermöwe (Larus argentatus) in die Untersuchungen einbezogen werden. Während des Untersuchungszeitraumes konnten so insgesamt 29 Parasitengattungen bzw. -arten nachgewiesen werden, denen z.T. kommerziell genutzte Süßwasserfische als Zwischenwirte oder paratenische Wirte dienen. Die Parasiten des Kormorans umfassten 15 verschiedene Parasitenarten. Dabei handelte es sich um die beiden Zestoden Ligula intestinalis und Paradilepis scolecina, die sechs Nematoden Baruscapillaria spp., Contracaecum rudolphii, Cyathostoma microspiculum, Desmidocercella incognita, Eucoleus contortus und Syncuaria squamata und die sieben Trematoden Clinostomum complanatum, Holostephanus dubinini, Hysteromorpha triloba, Metorchis xanthosomus, Paryphostomum radiatum, Petasiger exaeretus und P. phalacrocoracis. Außerdem wurden die zwei Kratzer Corynosoma strumosum und Polymorphus sp. nachgewiesen. Im Graureiher konnten dagegen nur neun Helminthenarten nachgewiesen werden. So der Zestode Neogryporhynchus cheilancristrotus, die sechs Trematoden Apharyngostrigea cornu, Bilharziella polonica, Diplostomum sp., Echinoasmus belocephalus, Posthodiplostomum cuticola und Tylodelphis excavata und die drei Nematoden Avioserpens galliardi, Desmidocercella incognita und Desmidocercella numidica. In der Silbermöwe konnten der Zestode Echinocotyle verschureni, die vier Trematoden Apophallus mühlingi, Diplostomum pseudospathaceum, Renicola lari und Metorchis xanthosomus sowie die drei Nematoden Desmidocercella incognita, Cosmocephalus obvelatus und Paracuaria tridentata nachgewiesen werden. Die parasitologischen Befunde des Silberreihers beschränkten sich auf Apharyngostrigea sp., Desmidocercella incognita und Desmidocercella numidica. Eine Beeinflussung der Helminthenfauna von Karpfen durch Kormoran und Reiher als auch umgekehrt konnte anhand der eigenen Untersuchungen in sächsischen Teichwirtschaften nicht sicher nachgewiesen werden. / In Saxonian carp ponds the cestodes Neogryporhynchus cheilancristrotus and Paradilepis scolecina and the trematode Diplostomum pseudospathaceum occur amongst others in carps (KAPPE 2004, PIETROCK et al. 2006, KAPPE & SEIFERT unpublished). Further fish species (tench, roach, rainbow trout) are infected with the trematodes Diplostomum sp., Hysteromorpha triloba and Posthodiplostomum cuticola (PIETROCK et al. 2006). This work was performed to analyse the importance of fish eating birds as vectors of fish pathogenic helminths. For this purpose 97 common cormorants (Phalacrocorax carbo) and 46 grey herons (Ardea cinerea) were examined on their infestation with endohelminths during the term of June 2003 to December 2004. All these birds came from the vicinity of Saxonian fish ponds. In winter 2004 additionally an egret (Casmerodius albus) and an hering gull (Larus argentatus) were included into the investigations. Altogether 29 parasite species and genus respectively could be detected. Some of these endohelminths use fresh water fishes as intermediate or paratenic hosts. The cormorants harboured 15 different parasite species and genus respectively: the two cestodes Ligula intestinalis and Paradilepis scolecina, the six nematodes Baruscapillaria carbonis, Contracaecum rudolphii, Cyathostoma microspiculum, Desmidocercella incognita, Eucoleus contortus and Syncuaria squamata and seven trematodes the Clinostomum complanatum, Holostephanus dubinini, Hysteromorpha triloba, Metorchis xanthosomus, Paryphostomum radiatum, Petasiger exaeretus and P. phalacrocoracis. In addition the two acanthocephales Corynosoma strumosum and Polymorphus sp. were detected. In the grey heron nine helminth species/genus could be detected: the cestode Neogryporhynchus cheilancristrotus, the six trematodes Apharyngostrigea cornu, Bilharziella polonica, Diplostomum sp., Echinoasmus belocephalus, Posthodiplostomum cuticola and Tylodelphis excavata and the three nematodes Avioserpens galliardi, Desmidocercella incognita and Desmidocercella numidica. In the hering gull the cestode Echinocotyle verschureni, the four trematodes Apophallus muehlingi, Diplostomum pseudospathaceum, Renicola lari and Metorchis xanthosomus as well as the three nematodes Desmidocercella incognita, Cosmocephalus obvelatus and Paracuaria tridentata were detected. Merely three endohelminths could be demonstrated in the investigated egret: Apharyngostrigea sp., Desmidocercella incognita and D. numidica. Six of the 29 endohelminth species and genus respectively were found in this examination to infest both fish and birds in Saxonian fish ponds. Nevertheless the helminth fauna of the inestigated birds, in particular of cormorants and herons did not make a definite impact on the carps´ infestation with endohelminths. As well as the fish helminth fauna were not unequivocally linked to those of the birds.

In Karpfen sächsischer Karpfenteichwirtschaften spielen u.a. die Zestoden Neogryporhynchus cheilancristrotus und Paradilepis scolecina und der Trematode Diplostomum pseudospathaceum eine Rolle (KAPPE 2004, PIETROCK et al. 2006, KAPPE & SEIFERT unveröff.). Weitere Fischarten (Schleie, Plötze, Regenbogenforelle) sind mit den Trematoden Diplostomum sp., Hysteromorpha triloba, Posthodiplostomum cuticola infiziert (PIETROCK et al. 2006). Mit dieser Arbeit sollte der Versuch unternommen werden, die Bedeutung Fisch fressender Vögel als Vektor Fisch pathogener Helminthen in Sachsen aufzuklären. Dafür wurden im Zeitraum von Juni 2003 bis Dezember 2004 97 Kormorane (Phalacrocorax carbo) und 46 Graureiher (Ardea cinerea) aus dem Einzugsgebiet sächsischer Teichwirtschaften auf ihren Befall mit Endohelminthen untersucht. Im Winter 2004 konnte zusätzlich ein Silberreiher (Casmerodius albus) und eine Silbermöwe (Larus argentatus) in die Untersuchungen einbezogen werden. Während des Untersuchungszeitraumes konnten so insgesamt 29 Parasitengattungen bzw. -arten nachgewiesen werden, denen z.T. kommerziell genutzte Süßwasserfische als Zwischenwirte oder paratenische Wirte dienen. Die Parasiten des Kormorans umfassten 15 verschiedene Parasitenarten. Dabei handelte es sich um die beiden Zestoden Ligula intestinalis und Paradilepis scolecina, die sechs Nematoden Baruscapillaria spp., Contracaecum rudolphii, Cyathostoma microspiculum, Desmidocercella incognita, Eucoleus contortus und Syncuaria squamata und die sieben Trematoden Clinostomum complanatum, Holostephanus dubinini, Hysteromorpha triloba, Metorchis xanthosomus, Paryphostomum radiatum, Petasiger exaeretus und P. phalacrocoracis. Außerdem wurden die zwei Kratzer Corynosoma strumosum und Polymorphus sp. nachgewiesen. Im Graureiher konnten dagegen nur neun Helminthenarten nachgewiesen werden. So der Zestode Neogryporhynchus cheilancristrotus, die sechs Trematoden Apharyngostrigea cornu, Bilharziella polonica, Diplostomum sp., Echinoasmus belocephalus, Posthodiplostomum cuticola und Tylodelphis excavata und die drei Nematoden Avioserpens galliardi, Desmidocercella incognita und Desmidocercella numidica. In der Silbermöwe konnten der Zestode Echinocotyle verschureni, die vier Trematoden Apophallus mühlingi, Diplostomum pseudospathaceum, Renicola lari und Metorchis xanthosomus sowie die drei Nematoden Desmidocercella incognita, Cosmocephalus obvelatus und Paracuaria tridentata nachgewiesen werden. Die parasitologischen Befunde des Silberreihers beschränkten sich auf Apharyngostrigea sp., Desmidocercella incognita und Desmidocercella numidica. Eine Beeinflussung der Helminthenfauna von Karpfen durch Kormoran und Reiher als auch umgekehrt konnte anhand der eigenen Untersuchungen in sächsischen Teichwirtschaften nicht sicher nachgewiesen werden. / In Saxonian carp ponds the cestodes Neogryporhynchus cheilancristrotus and Paradilepis scolecina and the trematode Diplostomum pseudospathaceum occur amongst others in carps (KAPPE 2004, PIETROCK et al. 2006, KAPPE & SEIFERT unpublished). Further fish species (tench, roach, rainbow trout) are infected with the trematodes Diplostomum sp., Hysteromorpha triloba and Posthodiplostomum cuticola (PIETROCK et al. 2006). This work was performed to analyse the importance of fish eating birds as vectors of fish pathogenic helminths. For this purpose 97 common cormorants (Phalacrocorax carbo) and 46 grey herons (Ardea cinerea) were examined on their infestation with endohelminths during the term of June 2003 to December 2004. All these birds came from the vicinity of Saxonian fish ponds. In winter 2004 additionally an egret (Casmerodius albus) and an hering gull (Larus argentatus) were included into the investigations. Altogether 29 parasite species and genus respectively could be detected. Some of these endohelminths use fresh water fishes as intermediate or paratenic hosts. The cormorants harboured 15 different parasite species and genus respectively: the two cestodes Ligula intestinalis and Paradilepis scolecina, the six nematodes Baruscapillaria carbonis, Contracaecum rudolphii, Cyathostoma microspiculum, Desmidocercella incognita, Eucoleus contortus and Syncuaria squamata and seven trematodes the Clinostomum complanatum, Holostephanus dubinini, Hysteromorpha triloba, Metorchis xanthosomus, Paryphostomum radiatum, Petasiger exaeretus and P. phalacrocoracis. In addition the two acanthocephales Corynosoma strumosum and Polymorphus sp. were detected. In the grey heron nine helminth species/genus could be detected: the cestode Neogryporhynchus cheilancristrotus, the six trematodes Apharyngostrigea cornu, Bilharziella polonica, Diplostomum sp., Echinoasmus belocephalus, Posthodiplostomum cuticola and Tylodelphis excavata and the three nematodes Avioserpens galliardi, Desmidocercella incognita and Desmidocercella numidica. In the hering gull the cestode Echinocotyle verschureni, the four trematodes Apophallus muehlingi, Diplostomum pseudospathaceum, Renicola lari and Metorchis xanthosomus as well as the three nematodes Desmidocercella incognita, Cosmocephalus obvelatus and Paracuaria tridentata were detected. Merely three endohelminths could be demonstrated in the investigated egret: Apharyngostrigea sp., Desmidocercella incognita and D. numidica. Six of the 29 endohelminth species and genus respectively were found in this examination to infest both fish and birds in Saxonian fish ponds. Nevertheless the helminth fauna of the inestigated birds, in particular of cormorants and herons did not make a definite impact on the carps´ infestation with endohelminths. As well as the fish helminth fauna were not unequivocally linked to those of the birds.

Orientador: José Paschoal Batistuti / Banca: Rubens Monti / Banca: Alice Yoshiko Tanaka / Resumo: Recentemente, a indústria de alimentos está preocupada em desenvolver novos produtos, usando ingredientes inovadores, que promovam benefícios à saúde. O uso de proteínas de leguminosas como uma alternativa para a proteína animal tem sido estudada. O tremoço branco (Lupinus albus L.) apresenta elevada concentração de proteína e óleo, principalmente ácidos graxos insaturados e poliinsaturados, fibra alimentar, açúcares e vitaminas B1 e B2. Por outro lado, o tremoço não pode ser consumido diretamente porque possui alcaloides, que além de serem tóxicos, atribuem gosto amargo ao grão. Como a maioria dos alcaloides é solúvel em água, a quantidade de alcaloide do grão pode ser diminuída através de água corrente, fervente ou salmoura. Sucos de frutas são bastante consumidos no Brasil, especialmente no verão, não somente pelo sabor, mas como fonte de hidratação e por serem ricos em nutrientes. Os sucos de frutas tropicais conquistam cada vez mais o mercado consumidor, sendo o Brasil, um dos principais produtores. A pitanga, fruto da pitangueira (Eugenia uniflora L.) é uma planta frutífera nativa do Brasil (das regiões Sul e Sudeste e que tem se adaptado favoravelmente às condições climáticas e edáficas da região Nordeste), da Argentina e do Uruguai. A pitanga é uma fruta cuja polpa apresenta excelentes condições para industrialização, devido ao seu alto rendimento, aroma agradável e sabor exótico. O objetivo do presente estudo foi o de verificar, através da metodologia de superfície de resposta, as melhores condições para a utilização simultânea de extrato aquoso de tremoço branco e de suco tropical de pitanga, no desenvolvimento de uma bebida. Para tanto, as variáveis independentes foram representadas pelo volume de extrato aquoso de tremoço (mL) e pelo volume de suco de pitanga... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Recently, the food industry is concerned about developing new products, using innovative ingredients that promote health benefits. The use of vegetable protein as an alternative to animal protein is being studied. The white lupine (Lupinus albus L.) has high concentrations of protein and oil, especially non saturated fatty acids and polynonsaturated fats, dietary fiber, sugars and vitamins B1 and B2. On the other hand, the lupine bean can't be consumed straight because it has alkaloids which besides being toxic, add a bitter taste to the bean. As most alkaloids are water soluble, the amount of alkaloid in the beans can be reduced by running water, boiling or brine. Fruit juices are widely consumed in Brazil, especially in the summer, not only for their flavor, but as a source of hydration and because they are rich in nutrients. The tropical fruit juices are conquering the market more and more, with Brazil being one of the main producers in the world. The Pitanga (Eugenia uniflora L.) is the fruit of the Pitangueira, a native plant of southern Brazil (which has adapted positively to weather and soil conditions in the country's Northeast), Argentina and Uruguay. Pitanga is a fruit which pulp presents excellent conditions for industrialization, due to its high yield, pleasant aroma and exotic flavor. The aim of the present study was to verify, through the response surface methodology, the optimum conditions for the combined use of aqueous extract of white lupine and tropical pitanga juice in the development of a drink. Therefore, the independent variables were represented by the volume of aqueous extract of lupine (mL) and by the volume of pitanga juice (mL). The dependent variables (responses) were obtained through sensory test of acceptance ("appearance", "aroma", "taste" and "overall acceptability"). The combined... (Complete abstract click electronic access below) / Mestre

[Truncated abstract] The phloem long distance translocation system is not only involved in the transport of nutrients and photo-assimilates to different organs of the plant, but it also appears to be important for the transport of information molecules including growth-regulators, proteins and RNA. Translocation of signals appears to be involved in the coordination of developmental processes and also in the response of the plant to environmental cues. Much of the information about macromolecules in phloem comes from analyses of exudates collected from the stylets of sap sucking insects or from incisions made to the vasculature. Among the legumes, members of the genus Lupinus exude phloem 'freely' from incisions made to the vasculature at most organs of the plant. This feature was exploited in this study to document some of the macromolecules present in exudate of L. albus and which might represent potential mobile signals. Phloem exudate was collected mainly from the sutures of developing pods and from inflorescence racemes. Two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry were used to identify 83 proteins in exudate. Analysis of a cDNA library constructed from exudate identified 609 unique transcripts. Both proteins and mRNA were classified into functional groups. The largest group was related to general and energy metabolism, suggesting some metabolic activity probably to support the sieve element (SE). Other significant functional groups were represented by proteins and transcripts involved in protein synthesis, turnover and sorting, and in redox homeostasis. Proteins in these categories could play a role in maintaining the functions and stability of proteins in SE. Macromolecules involved in signalling such as transcripts encoding proteins mediating calcium levels and the Flowering locus T (FT) protein were also identified in phloem exudate of L. albus. FT protein has been recently identified as a mobile signal that induces flowering. ... The hen1 mutant accumulates low, sometimes even undetectable levels of miRNA due to the lack of methylation. No translocation of the five miRNA assayed under nutrient replete (non stress) conditions was observed. Translocation of miR395 in response to sulphur (S) deficiency was also investigated, and while conclusive evidence of translocation was not obtained, the data suggested some movement from roots to shoots (possibly in xylem) of a signal in response to S-deficiency. Future work is required to provide greater insight into the translocation path and identity of this S-deficiency signal. This study suggests that not all miRNA identified in phloem exudates are mobile, which raises the question about their biological relevance in SE and how they reached this location (e.g. through the action of a non-selective transport mechanism). However, there is also the possibility that miRNA are translocated only in response to specific internal or external cues not tested in this study. This is the first study that provides information on macromolecules present in the phloem exudate of a member of the Fabaceae. The information obtained from this work, provides a basis for future studies in the identification of potential mobile signals that may play a role in a communication network that traffics information around the plant, regulating its various developmental processes and responding to environmental cues.

Os sistemas de produção agrícola atuais são em sua maioria ecologicamente impactantes e utilizam altas quantidades de recursos naturais. Nesse contexto, a produção de hortaliças é uma atividade que se caracteriza pelo uso intensivo da terra com alta demanda por insumos e requer a adoção de estratégias de manejo com enfoque agroecológico de modo a sustentar sua produção. Dessa maneira, tendo em vista a crise ambiental na qual o planeta se insere, há a necessidade de se buscar formas sustentáveis de produção. Com o intuito de se avaliar a viabilidade de se aliar duas práticas utilizadas em sistemas de produção sustentáveis, o presente trabalho teve como objetivo estudar o efeito da consorciação de adubos verdes com alface quanto ao seu desempenho agronômico. Foi utilizado delineamento experimental de blocos ao acaso, com parcelas subdivididas, e seis repetições. Os tratamentos consistiram de alface consorciada em linha e em sistema de adição com os adubos verdes aveia-preta (Avena strigosa Schreb.), caupi [Vigna unguiculata (L.) Walp.] e tremoço-branco (Lupinus albus L.). Os adubos verdes foram semeados em 4 épocas distintas anteriormente ao transplante da hortaliça, sendo que estas épocas foram 60, 40, 20 e 0 dias. Os parâmetros avaliados para o desempenho de alface foram peso fresco de cabeça, peso seco de cabeça, diâmetro de cabeça, altura de cabeça, número de folhas por cabeça, peso fresco de raiz, peso seco de raiz, comprimento de raiz e altura do pendão floral. Foram também avaliados peso fresco e peso seco dos adubos verdes. Concluiu-se que a alface não-consorciada exibiu desempenho superior para a maioria dos parâmetros avaliados. As espécies adubos verdes, bem como sua época de semeadura em relação ao estabelecimento do consórcio influenciaram de maneiras diferentes o desempenho da hortaliça. Dentre os adubos verdes, o tremoço-branco resultou em melhor desempenho da alface consorciada em comparação com o caupi. O plantio simultâneo da alface e dos adubos verdes resultou nos melhores desempenhos da hortaliça, e a semeadura dos adubos verdes com 40 dias de antecedência do transplante da alface resultou em desempenhos inferiores para a maioria dos parâmetros. / The agricultural production systems currently used are in most cases ecologically impacting and use high amounts of natural resources. Within this context, vegetable production is an activity characterized by intensive land use and high demand for farm inputs, requiring the adoption of an agroecological management approach for sustaining its production. Thus, with the environmental crisis which the planet is in, it is necessary to search for healthy agriculture methods. With the purpose of evaluating the viability of joining two sustainable agriculture practices, the present work aimed at studying the effect of intercropping green manures with lettuce in its performance. A randomized complete blocks design, with six replicates was used. Treatments consisted of lettuce intercropped in rows in additive design with the green manures black oat (Avena strigosa Schreb.), cowpea [Vigna unguiculata (L.) Walp.] and white lupin (Lupinus albus L.). Intercropping was established in four different green manure sowing dates (60, 40, 20 and 0) prior to lettuce transplanting. Measurements in lettuce included fresh weight, dry weight, number of leaves, diameter of head, length of head, root fresh weight, root dry weight, root length and length of bolting plants. Measurements in green manures included fresh and dry weight. The monocropped lettuces had a better performance than the intercropped ones in most evaluated parameters. Green manure species and sowing date prior to lettuce transplant had different effects on the vegetable performance. Among the green manures, white lupin resulted in a better intercropped lettuce performance compared to cowpea. The simultaneous lettuce and green manures planting resulted in a better vegetable performance. When the green manures were sowed 40 days before lettuce transplanting the vegetable had its worst performance for most evaluated parameters.

Comme chez de nombreux protéagineux, l'architecture déterminée a semblé une voie prometteuse pour réduire le développement végétatif et améliorer le rendement et la stabilité du rendement du lupin blanc d'hiver (Lupinus albus L.). Une étude à la fois génétique et physiologique de ce type architecturale et de ses conséquences sur le développement et la mise en place du rendement a donc été entreprise. L'hérédité du caractère d'architecture déterminée est monogénique récessive, ce qui permet une utilisation simple en sélection. Le développement végétatif est réduit car tous les bourgeons passent à l'état floral précocément dans le cycle. Les ramifications portent chacune moins de feuilles que chez les indéterminés, et le nombre de niveaux végétatifs est réduit. La distribution des feuilles sur les ramifications suit un profil caractéristique en forme de cloche. La structure des ramifications a pu être modélisée. Il existe une grande variabilité génétique pour l'architecture, bien que la relation positive entre tardiveté de floraison et développement végétatif soit forte. L'interception de la lumière par le couvert en fonction du temps est similaire chez les déterminés et les indéterminés. Cependant, les déterminés atteignent une interception maximale moins importante en raison de leur développement végétatif restreint. La proportion de lumière qui parvient jusqu'aux feuilles de la tige principale est accrue. Le rendement des génotypes déterminés semble compétitif avec celui des génotypes indéterminés. La production de matière sèche est plus faible mais l'indice de récolte est supérieur. La date de maturité est sensiblement avancée, surtout sous des climats frais et humides, et la stabilité du rendement est plus grande. Ces caractéristiques sont liées à la réduction du développement végétatif, et à une compétition entre développement végétatif et développement reproducteur plus faible que celle observée chez les indéterminés. Le rendement est produit essentiellement sur la tige principale et le premier niveau de ramifications, et ces sites sont moins sujets à des aléas climatiques que les niveaux supérieurs. La variabilité génétique pour les composantes du rendement est large. On met en évidence des relations entre certains caractères d'architecture et les potentialités de rendement. Un développement végétatif trop restreint aussi bien qu'un développement excessif nuisent au rendement. Les caractères de développement des ramifications (nombre de feuilles et nombre de niveaux végétatifs) sont des critères de sélection pertinents chez les lupins déterminés.

Research developing artificial propagation, husbandry, and nutrition of sturgeon species has developed greatly since the collapse of the Caspian Sea fisheries in the 1980s. Sturgeon species are commercially prized for their unfertilized roe which is marketed as the delicacy, caviar. Sturgeon production units commonly utilize commercial salmonid feeds, which contain large proportions of fish meal (FM) and fish oil (FO). Concerns regarding economics and sustainability have put pressure on aquafeed manufactures to efficiently utilize FM and FO, and to incorporate alternative protein sources, such as soybean meal (SBM). Therefore, the present studies estimated the optimal protein:energy ratio of juvenile Pallid Sturgeon Scaphirhynchus albus and evaluated the effects of increasing SBM composition on growth, feed efficiency, body and liver composition, and intestinal morphology of juvenile Shovelnose Sturgeon Scaphirhynchus platorhynchus, two species of Scaphirhynchus sturgeon of regional commercial importance. Protein:energy ratios were investigated using casein and dextrose based, semi-purified diets. Results indicated that Scaphirhynchus sturgeon are able to perform similarly across a wide range of protein:energy ratios (79 - 147 mg protein kcal-1), so long as adequate dietary energy (3,800 kcal kg-1 gross energy) is provided and essential amino acids are not limiting. Soybean meal was evaluated utilizing practical, isocaloric, isolipidic, isoenergetic test diets, designed to replace FM with increasing SBM. Juvenile Scaphirhynchus sturgeon were found to perform similarly on diets with up to 50% of FM replaced with SBM (336 g kg-1 diet). Evaluation of liver composition revealed that juvenile Shovelnose Sturgeon store less glycogen and crude lipid than other fish species, although no dietary differences were observed. Histological evaluation of the distal intestine revealed indications of SBM induced enteritis, though no statistical differences in measures were detected between treatments. This research is the first to describe optimal dietary formulation for the culture of Scaphirhynchus sturgeon.

Os sistemas de produção agrícola atuais são em sua maioria ecologicamente impactantes e utilizam altas quantidades de recursos naturais. Nesse contexto, a produção de hortaliças é uma atividade que se caracteriza pelo uso intensivo da terra com alta demanda por insumos e requer a adoção de estratégias de manejo com enfoque agroecológico de modo a sustentar sua produção. Dessa maneira, tendo em vista a crise ambiental na qual o planeta se insere, há a necessidade de se buscar formas sustentáveis de produção. Com o intuito de se avaliar a viabilidade de se aliar duas práticas utilizadas em sistemas de produção sustentáveis, o presente trabalho teve como objetivo estudar o efeito da consorciação de adubos verdes com alface quanto ao seu desempenho agronômico. Foi utilizado delineamento experimental de blocos ao acaso, com parcelas subdivididas, e seis repetições. Os tratamentos consistiram de alface consorciada em linha e em sistema de adição com os adubos verdes aveia-preta (Avena strigosa Schreb.), caupi [Vigna unguiculata (L.) Walp.] e tremoço-branco (Lupinus albus L.). Os adubos verdes foram semeados em 4 épocas distintas anteriormente ao transplante da hortaliça, sendo que estas épocas foram 60, 40, 20 e 0 dias. Os parâmetros avaliados para o desempenho de alface foram peso fresco de cabeça, peso seco de cabeça, diâmetro de cabeça, altura de cabeça, número de folhas por cabeça, peso fresco de raiz, peso seco de raiz, comprimento de raiz e altura do pendão floral. Foram também avaliados peso fresco e peso seco dos adubos verdes. Concluiu-se que a alface não-consorciada exibiu desempenho superior para a maioria dos parâmetros avaliados. As espécies adubos verdes, bem como sua época de semeadura em relação ao estabelecimento do consórcio influenciaram de maneiras diferentes o desempenho da hortaliça. Dentre os adubos verdes, o tremoço-branco resultou em melhor desempenho da alface consorciada em comparação com o caupi. O plantio simultâneo da alface e dos adubos verdes resultou nos melhores desempenhos da hortaliça, e a semeadura dos adubos verdes com 40 dias de antecedência do transplante da alface resultou em desempenhos inferiores para a maioria dos parâmetros. / The agricultural production systems currently used are in most cases ecologically impacting and use high amounts of natural resources. Within this context, vegetable production is an activity characterized by intensive land use and high demand for farm inputs, requiring the adoption of an agroecological management approach for sustaining its production. Thus, with the environmental crisis which the planet is in, it is necessary to search for healthy agriculture methods. With the purpose of evaluating the viability of joining two sustainable agriculture practices, the present work aimed at studying the effect of intercropping green manures with lettuce in its performance. A randomized complete blocks design, with six replicates was used. Treatments consisted of lettuce intercropped in rows in additive design with the green manures black oat (Avena strigosa Schreb.), cowpea [Vigna unguiculata (L.) Walp.] and white lupin (Lupinus albus L.). Intercropping was established in four different green manure sowing dates (60, 40, 20 and 0) prior to lettuce transplanting. Measurements in lettuce included fresh weight, dry weight, number of leaves, diameter of head, length of head, root fresh weight, root dry weight, root length and length of bolting plants. Measurements in green manures included fresh and dry weight. The monocropped lettuces had a better performance than the intercropped ones in most evaluated parameters. Green manure species and sowing date prior to lettuce transplant had different effects on the vegetable performance. Among the green manures, white lupin resulted in a better intercropped lettuce performance compared to cowpea. The simultaneous lettuce and green manures planting resulted in a better vegetable performance. When the green manures were sowed 40 days before lettuce transplanting the vegetable had its worst performance for most evaluated parameters.